Digital Commons Network^™

Investigating The Relationship Between Smoking, Genomics, And Brain Imaging, Yoonhoo Chang

Arts & Sciences Graduate Student Theses and Dissertations

Cigarette smoking has been linked to adverse health outcomes, including several forms of cancer, respiratory and cardiovascular disease, and dementia. Despite the public health campaigns aimed at reducing tobacco use, the behavior remains prevalent, and its association with various organs is an active area of research. Recent studies showed that smoking behaviors have strong genetic contributions. Through genome-wide association studies, the genetic risk score of individuals can be created and used as a basis for more effective and precise healthcare solutions. Chapter 2 explores the creation of a polygenic risk score (PRS) for smoking cessation and its utility in two …

Interleukin-17-Mediated Protective Cytokine Signaling Against Degeneration Of The Retinal Pigment Epithelium, Yan Chen, Sarah E Bounds, Xiang Ma, James Regun Karmoker, Yin Liu, Jian-Xing Ma, Jiyang Cai

Faculty, Staff and Student Publications

Injuries to the retinal pigment epithelium (RPE) and outer retina often result in the accumulation of retinal microglia within the subretinal space. These subretinal microglia play crucial roles in inflammation and resolution, but the mechanisms governing their functions are still largely unknown. Our previous research highlighted the protective functions of choroidal γδ T cells in response to RPE injury. In the current study, we employed single-cell RNA sequencing approach to characterize the profiles of immune cells in mouse choroid. We found that γδ T cells were the primary producer of interleukin-17 (IL-17) in the choroid. IL-17 signaled through its receptor …

A New Mother’S Cry: Analyzing Traditional Social Support Versus Online Social Support In Relation To The Postpartum Crisis, Emily Hughes

Doctoral Dissertations and Projects

The present study examines the gap that remains involving the transition from traditional to online social support that many mothers have participated in and how this change in communication methods during postpartum recovery may contribute to mental health disorders. This qualitative study aims to examine the differences in lived experiences among mothers regarding in-person and online forms of social support and how these experiences may impact a new mother’s mental well-being. Based on previous research, the present study hypothesizes that a more significant amount of online social support compared to in-person support will result in a detriment to a new …

Healing Artists Through Art, Corey Aaron Cundiff

Masters Theses

Men die by suicide nearly four times more than women, with higher rates among artists. There has been an increase in the rate of suicide over the years, primarily linked to depression and mounting societal expectations imposed upon men. The prevalence of social media and the awareness that comes with indicates that the issue of suicide may never disappear unless action is taken. This paper examines the need for art therapy to counter depression among male artists. No longer do we need to rely strictly on medication and talk therapy to treat depression. Many art therapy techniques have proven quite …

Overview Of Urinary Tract Infection Caused By Bacteria, Ahmed Talib Abdulkarem, Shaimaa M.S. Zainulabdeen, Saif Mazeel Abed

Muthanna Medical Journal

Urinary tract infections [UTIs], a significant public health concern, can be brought on by a wide variety of bacteria, including Escherichia coli, Proteus mirabilis, Enterococcus faecalis, Klebsiella pneumoniae, and Staphylococcus saprophyticus. The high recurrence rates and evolving antibiotic resistance of uropathogens constitute a serious threat to the financial burden of these disorders. Pathogens in urine can be detected using a variety of diagnostic approaches, which are broadly divided into laboratory-based and point-of-care [POC] detection methods. Many research institutions and companies working in this subject have strived to establish a rapid and accurate pathogen identification because traditional approaches may be time-consuming. …

Following The Paper Trail: An Ethnoecology Of Hand Papermaking Traditions, James Ojascastro

Arts & Sciences Graduate Student Theses and Dissertations

Plants and their fibers have served as simple yet incredibly versatile raw materials for innovation for as long as humans have been on the planet. As the principal structural building blocks of plant cell walls, these hollow, cellulosic fibers have been co-opted by cultures around the world to meet diverse structural, absorptive, motile, and thermoregulatory needs—especially as textiles and paper. Despite (or because of!) this versatility, fibers vary considerably in physiology, and ethnographic records show that very few plant species have been chosen by tradition for use in fiber products. Surprisingly, no fiber ethnobotany study to date has attempted to …

Life, The Genome And Everything, Susan M Rosenberg

Faculty, Staff and Students Publications

In this issue of the Journal of Bacteriology, N. J. Bonde, E. A. Wood, K. S. Myers, M. Place, J. L. Keck, and M. M. Cox (J Bacteriol 205:e00184-23, 2023, https://doi.org/10.1128/jb.00184-23) used an unbiased transposon-sequencing (Tn-seq) screen to identify proteins required for life when cells lose the RecG branched-DNA helicase (synthetic lethality). The proteins’ identities indicate pathways that prevent endogenous DNA damage, pathways that prevent its homology-directed repair (HDR) “strand-exchange” intermediates between sister chromosomes, and pathways that resolve those intermediates. All avoid intermediate pile-up, which blocks chromosome segregation, causing “death-by-recombination.” DNA damage is managed to regulate crucial but …

Prmt Blockade Induces Defective Dna Replication Stress Response And Synergizes With Parp Inhibition, Yang Li, Lacey E Dobrolecki, Christina Sallas, Xudong Zhang, Travis D Kerr, Deepa Bisht, Yalong Wang, Sharad Awasthi, Babita Kaundal, Siqi Wu, Weiyi Peng, Marc L Mendillo, Yiling Lu, Collene R Jeter, Guang Peng, Jinsong Liu, Shannon N Westin, Anil K Sood, Michael T Lewis, Jishnu Das, S Stephen Yi, Mark T Bedford, Daniel J Mcgrail, Nidhi Sahni

Faculty, Staff and Student Publications

Multiple cancers exhibit aberrant protein arginine methylation by both type I arginine methyltransferases, predominately protein arginine methyltransferase 1 (PRMT1) and to a lesser extent PRMT4, and by type II PRMTs, predominately PRMT5. Here, we perform targeted proteomics following inhibition of PRMT1, PRMT4, and PRMT5 across 12 cancer cell lines. We find that inhibition of type I and II PRMTs suppresses phosphorylated and total ATR in cancer cells. Loss of ATR from PRMT inhibition results in defective DNA replication stress response activation, including from PARP inhibitors. Inhibition of type I and II PRMTs is synergistic with PARP inhibition regardless of homologous …

Rotavirus-Mediated Dgat1 Degradation: A Pathophysiological Mechanism Of Viral-Induced Malabsorptive Diarrhea, Zheng Liu, Hunter Smith, Jeanette M Criglar, Antonio J Valentin, Umesh Karandikar, Xi-Lei Zeng, Mary K Estes, Sue E Crawford

Faculty, Staff and Students Publications

Gastroenteritis is among the leading causes of mortality globally in infants and young children, with rotavirus (RV) causing ~258 million episodes of diarrhea and ~128,000 deaths annually in infants and children. RV-induced mechanisms that result in diarrhea are not completely understood, but malabsorption is a contributing factor. RV alters cellular lipid metabolism by inducing lipid droplet (LD) formation as a platform for replication factories named viroplasms. A link between LD formation and gastroenteritis has not been identified. We found that diacylglycerol O-acyltransferase 1 (DGAT1), the terminal step in triacylglycerol synthesis required for LD biogenesis, is degraded in RV-infected cells by …

Mirna-211 Maintains Metabolic Homeostasis In Medulloblastoma Through Its Target Gene Long-Chain Acyl-Coa Synthetase 4, Menglang Yuan, Iqbal Mahmud, Keisuke Katsushima, Kandarp Joshi, Olivier Saulnier, Rudramani Pokhrel, Bongyong Lee, Wathsala Liyanage, Haritha Kunhiraman, Stacie Stapleton, Ignacio Gonzalez-Gomez, Rangaramanujam M Kannan, Tanja Eisemann, Elayaraja Kolanthai, Sudipta Seal, Timothy J Garrett, Saed Abbasi, Kimberly Bockley, Justin Hanes, Prem Chapagain, George Jallo, Robert J Wechsler-Reya, Michael D Taylor, Charles G Eberhart, Animesh Ray, Ranjan J Perera

Faculty, Staff and Students Publications

The prognosis of childhood medulloblastoma (MB) is often poor, and it usually requires aggressive therapy that adversely affects quality of life. microRNA-211 (miR-211) was previously identified as an important regulator of cells that descend from neural cells. Since medulloblastomas primarily affect cells with similar ontogeny, we investigated the role and mechanism of miR-211 in MB. Here we showed that miR-211 expression was highly downregulated in cell lines, PDXs, and clinical samples of different MB subgroups (SHH, Group 3, and Group 4) compared to normal cerebellum. miR-211 gene was ectopically expressed in transgenic cells from MB subgroups, and they were subjected …

Advanced Structural Brain Aging In Preclinical Autosomal Dominant Alzheimer Disease, Peter R Millar, Brian A Gordon, Julie K Wisch, Tammie L.S. Benzinger, Carlos Cruchaga, Jason J Hassenstab, Laura Ibanez, Celeste Karch, Jorge J Llibre-Guerra, John C Morris, Richard J Perrin, Charlene Supnet-Bell, Chengjie Xiong, Randall J Bateman, Beau M Ances, Eric M Mcdade, Et Al.

2020-Current year OA Pubs

BACKGROUND: "Brain-predicted age" estimates biological age from complex, nonlinear features in neuroimaging scans. The brain age gap (BAG) between predicted and chronological age is elevated in sporadic Alzheimer disease (AD), but is underexplored in autosomal dominant AD (ADAD), in which AD progression is highly predictable with minimal confounding age-related co-pathology.

METHODS: We modeled BAG in 257 deeply-phenotyped ADAD mutation-carriers and 179 non-carriers from the Dominantly Inherited Alzheimer Network using minimally-processed structural MRI scans. We then tested whether BAG differed as a function of mutation and cognitive status, or estimated years until symptom onset, and whether it was associated with established …

Review Of: In Plain View: The Daily Lives Of Amish Women—Judith Stavisky, Barbie Stoltzfus

Journal of Amish and Plain Anabaptist Studies

This book is an accurate snapshot of the Amish lifestyle. It is almost uncanny how Stavisky picked up on the inner mechanisms of the lifestyle of the largest and most progressive Amish settlement in the United States. Stavisky portrayed the lifestyle with respect and, for the most part, truth. The simple fact that the Amish allowed Stavisky into their lives and homes speaks much about the author’s tact and poise. [First paragraph.]

Review Of: Till Sunday Comes Again—D.E. Martin, Cory Anderson

Journal of Amish and Plain Anabaptist Studies

Bob Weaver owns one of those “Dutch Bucket” restaurants, that giant palace of Amish country, tourist-sustained eateries serving buffets of fried chicken, mashed potatoes mixed with heavy loads of sour cream, oily vegetables, and gooey gluten rolls. Bob has done an incredible business, and now he is opting to open on Sunday. He makes this move in the context of the ever-more-brittle convictions of his local conservative Anabaptist church. His piety juices out as he explains to his “buddy Dave” (p. 20), whom he hired to print out a new banner announcing Sunday brunch hours. [First paragraph.]

Partnering With The Old Order Mennonites In The Finger Lakes Region Of New York State To Identify The Mechanisms Of Protective Immunity Against Atopic Disease Development, Alexandra N. Kaplan, Camille A. Martina, Elizabeth R. Catlin, John Looney, Kirsi M. Järvinen

Journal of Amish and Plain Anabaptist Studies

Old Order Mennonites (OOM) follow a traditional agrarian lifestyle; key aspects include home births, large families, limited antibiotic usage, consumption of whole foods and unpasteurized milk, and early exposure to soil, stables, and farm animals. There is evidence that a farming lifestyle protects against asthma and allergies, yet the biological mechanisms behind this protective effect remain unknown. The “Zooming into Old Order Mennonites” (ZOOM) cohort study was established to further explore protective factors and mechanisms. This study compares immune development among OOM children from the Finger Lakes Region of New York to those residing 65 miles northwest in Rochester, NY. …

Assessing The Attitudes And Perceptions Of The Old Order Amish Toward Genetic Testing For Familial Defective Apolipoprotein B-100, Erin Sutcliffe, Laura J. Conway, Alan Shuldiner, Toni Pollin

Journal of Amish and Plain Anabaptist Studies

Through a founder effect, one in eight Lancaster County, PA, Amish harbors the pathogenic APOB variant R3527Q, causing Familial Defective Apolipoprotein B-100 (FDB) that is rare in the general population. Affected individuals are at increased risk for high low density lipoprotein (LDL) cholesterol levels and cardiovascular disease and may benefit from early screening and treatment. A qualitative interview approach was used to investigate the genetic risk perceptions of the Amish and their attitudes towards genetic testing for FDB. The goal was to identify elements necessary for a successful program that would identify high risk individuals in the Lancaster County Amish …

Direct Relationships Between The Five Internal Senses: The Extremes And In-Between Of The Inner Experience, Sydnie Hoyt, Camryn O'Neal, Miranda Brannum, Sara Bagley

The Confluence

Inner experience of all 5 modalities were investigated to determine if there were correlations amongst them and how visual mental imagery and internal hearing were used in an applied story. Our sample (N = 137) completed the Vividness of Visual Imagery Questionnaire (VVIQ; Marks, 1973) and the Plymouth Sensory Imagery Questionnaire (Psi-Q; Andrade et al., 2013) to obtain trait measures of the different modalities within the inner experience. Results showed statistically significant positive correlations between each of the trait measures of the 5 sensory modalities (visual mental imagery, inner hearing, taste, smell, and touch). Based on their VVIQ total scores, …

Lipid Membrane Self-Assembly And Synthesis In The Lab By Rapid, Low-Cost Microfluidics Approaches, Kathleen Hardwick, Shane O'Reilly

SURE Journal: Science Undergraduate Research Experience Journal

Lipid membranes, which self-assemble in nature and under laboratory conditions, can form vesicles called liposomes. The main uses for liposomes are in drug delivery systems and as animal cell models. From the initial research into lipid membrane science, to the systematic literature review using Web of Science, to refining the project scope, to the description of the method, this project follows the process of developing a method for lipid membrane self-assembly and synthesis in the lab. The result was a rapid prototyping method for fabricating microfluidic devices to test different designs for channels, for use with a liposome synthesis protocol. …

An Investigation Into Dietary Fibre Intake, Bowel Function And Mood Among A Sample Of Irish Adults, Sophie Mulligan, Ellen Lynch, Suzanne Doyle

SURE Journal: Science Undergraduate Research Experience Journal

Background

Previous studies have examined the relationship between dietary fibre intake and mood and bowel function separately, however, no cross-sectional study has yet explored the relationship between all three variables. This study examines the association between dietary fibre intake, bowel function and mood in a cohort of Irish adults at a university campus.

Methods

A cross-sectional study was conducted. An online questionnaire was distributed to staff and students of TU Dublin through email and publicised using posters. Three validated surveys were used in the design of the questionnaire, to assess dietary fibre intake, mood and bowel function. All staff and …

Regulation Of Mirnas In Obesity And Diabetes, Eva Maher, Ava O'Meara, Cathy Brougham

SURE Journal: Science Undergraduate Research Experience Journal

Introduction: The prevalence of obesity and obesity-related secondary disorders has increased dramatically in recent years. Dysregulated microRNA (miRNA) expression has been linked to the development of obesity and its associated disorders. Adipose derived stem cells (ADSCs) are promising cell models of obesity, obesity-related inflammation and diabetes. Furthermore, miRNA expression profiles have emerged as promising targets for the novel treatment of diseases such as obesity. In order to enhance miRNA-based therapies, the miRNA expression profile of obesity and its related disorders must be monitored to further understand the underlying mechanisms of obesity and to identify novel targets.

Aim: The aim of …

The Potential Of Stem Cell Therapy In Multiple Sclerosis Treatment: A Review, Sorina Amarculesei, Ava O'Meara Cushen, Cathy Brougham

SURE Journal: Science Undergraduate Research Experience Journal

Multiple Sclerosis is an autoimmune and neurodegenerative disease of the central nervous system. There are currently 2.8 million people living with Multiple Sclerosis worldwide, including 9000 people in Ireland, with a prevalence of 193 per 100,000 people. Symptoms of Multiple Sclerosis include sensory loss, fatigue, cognitive dysfunction, spasticity, depression, optic neuritis, gait ataxia, diplopia, and loss of bladder control. Currently, there is no standardised treatment or cure for Multiple Sclerosis with many strategies focusing on symptoms. Stem cells have emerged as promising approaches for Multiple Sclerosis therapeutics. Clinical trials primarily focus on mesenchymal stem cell-based therapies for Multiple Sclerosis. Furthermore, …

Tiny But Mighty, An Exploration Of Microbes And Plants As Sources Of Small Novel Bioactive Compounds Against Invasive Mycoses., Hannah Mai Peacock, James Anthony O'Connor

SURE Journal: Science Undergraduate Research Experience Journal

Despite the high mortality rate involved with invasive cerebral mycoses, there is a relative lack of research available around antifungals capable of crossing the blood brain barrier.

In this study the metabolites of four plants (Crocus vernus, Miniature Narcissus (daffodil), Osmanthus delavayi and Taraxacum officinale (common dandelion)) and two bacteria (Lacticaseibacillus paracasei and Staphylococcus epidermidis) were extracted and assayed for antifungal activity against Candida albicans and Saccharomyces cerevisiae. Thin layer chromatography and bioautography were then employed to assess the activity of the constituent components of sources showing antifungal activity.

Metabolites of S.epidermidis, and extracts …

Melittin: A Natural Component Of Honeybee Venom As A Potential Anti-Cancer Therapy, Niamh Donnellan, Anne M. Friel

SURE Journal: Science Undergraduate Research Experience Journal

Cancer is a major cause of death worldwide and while chemotherapy is the main approach there are many negative associations in current treatment procedures. These include lack of selectivity, side effects and drug resistance. The hallmarks of cancer are a fundamental concept which aids the development of new means to treat human cancers through the understanding of the acquisition of these hallmarks from cells.

Melittin is a major peptide component of bee venom which has shown to be efficacious as an anticancer agent in preclinical and animal models. Melittin has many biological functions including pore formation in the phospholipid bilayer …

Editorial - Sure Journal Vol 5, Anne M. Friel

SURE Journal: Science Undergraduate Research Experience Journal

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Ungrading: Reflections Through A Feminist Pedagogical Lens, Erin M. Eggleston, Shelby Kimmel

Feminist Pedagogy

Ungrading is a pedagogical approach in which no grades are given on any assignments. Instead, students are provided with many opportunities to submit work and gain feedback. The goal is to shift student focus from achieving a grade to growth as a learner and a person. As instructors, our ungrading approach utilized personalized learning plans, checkpoint reflections, and student-professor learning conferences to put agency in the hands of our students. We employed this method in upper-level biology and computer science courses and provide critical reflections here regarding our experiences and the connections between this approach and feminist STEM pedagogy tenets. …

Feminist Biology: Towards Gender Equity In The Biology Curriculum, Nicole Danos, Carla Y. Bonilla, Sofia Leung

Feminist Pedagogy

The current curriculum in STEM is a product of historically unequal representation of genders in the science community. As a result, most attention has been given to male biology, creating a knowledge gap that has affected our social and political perspectives, such as an underinvestment in women’s health research. Feminist biology seeks to provide equal time and weight to the impact of sex as a biological factor, using inclusive definitions of biological sex that go beyond the male/female binary. Feminist pedagogy is a method of teaching that involves engaged learning and reflection in order to create a community of learners …

Centering Equity In Stem Teaching: Stem Ideas That Change The World, Ileana Vasu

Feminist Pedagogy

No discussion on equity/inequity makes sense without bringing power into that discussion. As instructors we need to ask questions such as “who decides and controls what knowledge is”, “whose identities are empowered and whose are erased”, “who has access and opportunity and who doesn’t”. Traditional teaching in STEM, including mathematics, assumes knowledge is objective, transmittable, repeatable to everyone. When educators follow a traditional curriculum, just like their teachers before them, they do so thinking their methods ensure equality and objectivity. These practices not only deny the role that Western patriarchal cultures have played in creating these so-called equitable practices, but …

Creating Meaningful Connections With The Electron Transport Chain Beyond A Virtual Classroom, Lesley-Ann Giddings

Feminist Pedagogy

At the center of feminist pedagogy is community. Creating community within the classroom and between the class and local communities increases student engagement and access to knowledge. Communities can be a source of solidarity and further decenter authority in the classroom, empowering students, teachers, and the surrounding community to be co-learners. Amid the COVID-19 pandemic, remote teaching posed challenges to student engagement. Here, I describe an assignment used to build an inclusive community of co-learners within a virtual biochemistry class and our local communities. Oxidative phosphorylation is a cellular process used to create energy that depends on the electron transport …

Feminist Pedagogy In Stem: The Intersection Of Stem Pedagogy And Feminist Theory, Lesley-Ann Giddings, Candice R. Price

Feminist Pedagogy

No abstract provided.

The Effects Of Volcanic Deposits On Human Preservation, Charles M. Nuncio

Culture, Society, and Praxis

No abstract provided.

Nucleus Accumbens Core Single Cell Ensembles Bidirectionally Respond To Experienced Versus Observed Aversive Events, Oyku Dinckol, Noah Harris Wenger, Jennifer E Zachry, Munir Gunes Kutlu

Rowan-Virtua School of Osteopathic Medicine Departmental Research

Fear learning is a critical feature of survival skills among mammals. In rodents, fear learning manifests itself through direct experience of the aversive event or social transmission of aversive stimuli such as observing and acting on conspecifics' distress. The neuronal network underlying the social transmission of information largely overlaps with the brain regions that mediate behavioral responses to aversive and rewarding stimuli. In this study, we recorded single cell activity patterns of nucleus accumbens (NAc) core neurons using in vivo optical imaging of calcium transients via miniature scopes. This cutting-edge imaging methodology not only allows us to record activity patterns …