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Nitrogen Cycling In The Lower Chesapeake Bay And Mid- And South Atlantic Bight, Yifan Zhu

OES Theses and Dissertations

Nitrogen (N) is central to marine primary production; its availability often limits the capacity and rates of primary productivity in most of the world’s oceans. Contrastingly, estuaries frequently receive anthropogenic N loading, oftentimes resulting in eutrophication, harmful algal blooms (HABs), and substantially degraded water quality. Nutrient variability in both estuaries and oceanic regimes results from meteorological forcing and physical processes, including wind-induced, tidal, and mesoscale mixing and upwelling. In this dissertation, a comprehensive investigation of N variability and cycling and its links to physical-biogeochemical processes was conducted using time-series monitoring approaches, flux estimations, satellite imaging, biogeochemical measurements, and molecular analyses. …

Application Of Business Analytics Approaches To Address Climate-Change-Related Challenges, Donald J. Jenkins

Graduate Doctoral Dissertations

Climate change is an existential threat facing humanity, civilization, and the natural world. It poses many multi-layered challenges that call for enhanced data-driven decision support methods to help inform society of ways to address the deep uncertainty and incomplete knowledge on climate change issues. This research primarily aims to apply management, decision, information, and data science theories and techniques to propose, build, and evaluate novel data-driven methodologies to improve understanding of climate-change-related challenges. Given that we pursue this work in the College of Management, each essay applies one or more of the three distinct business analytics approaches (i.e., descriptive, prescriptive, …

Western Pearlshell (Margaritifera Falcata) Extirpation In The Smith River, Montana, With A Possible Link To Warming Water Temperatures, David M. Stagliano

Western North American Naturalist

Western pearlshell (Margaritifera falcata) populations are in decline across the species’ range in western North America. In 2008, Montana categorized the western pearlshell as an imperiled species of concern (S2) based on reduced detections at historically occupied sites and few viable populations. Between 2004 and 2014, we surveyed 24 Smith River reaches (61 total sites in the watershed) for the presence of western pearlshell populations. Despite historical and anecdotal descriptions of abundant western pearlshell in the Smith River, intensive structured surveys between 2004 and 2007 documented only 4 sections with low-density, nonviable western pearlshell populations (<10 individuals, large size classes [average total length = 70 mm], with no signs of recruitment). In 2014, we revisited these occupied sites to repeat surveys (expending significantly more search time) and observed no live individuals. This was surprising given that, although those populations would certainly die out without recruitment, older individuals can oftentimes persist for decades. Therefore, we consider the western pearlshell mussel extirpated from the Smith River watershed. We reviewed long-term USGS gage discharge and temperature data near the western pearlshell populations to evaluate the potential causal factors for, and timing of, the extirpation. From 3 July to 3 August 2007, water temperatures were at or above the thermal stress levels of the western pearlshell (>25 °C, …

Gamma Secretase Activating Protein Promotes End-Organ Dysfunction After Bacterial Pneumonia, Meredith S. Gwin, Mikhail F. Alexeyev, Aron M. Geurts, Ji Young Lee, Chun Zhou, Xi-Ming Yang, Michael V. Cohen, James M. Downey, Robert A. Barrington, Domenico Spadafora, Jonathon P. Audia, Dara W. Frank, Sarah Voth, Viktoriya V. Pastukh, Jessica Bell, Linn Ayers, Dhananjay T. Tambe, Amy R. Nelson, Ron Balczon, Mike T. Lin, Troy Stevens

University Faculty and Staff Publications

Pneumonia elicits the production of cytotoxic beta amyloid (Ab) that contributes to end-organ dysfunction, yet the mechanism(s) link- ing infection to activation of the amyloidogenic pathway that produces cytotoxic Ab is unknown. Here, we tested the hypothesis that gamma-secretase activating protein (GSAP), which contributes to the amyloidogenic pathway in the brain, promotes end-organ dys- function following bacterial pneumonia. First-in-kind Gsap knockout rats were generated. Wild-type and knockout rats possessed simi- lar body weights, organ weights, circulating blood cell counts, arterial blood gases, and cardiac indices at baseline. Intratracheal Pseudomonas aeruginosa infection caused acute lung injury and a hyperdynamic circulatory state. …

Gamma Secretase Activating Protein Promotes End-Organ Dysfunction After Bacterial Pneumonia, Meredith S. Gwin, Mikhail F. Alexeyev, Aron M. Geurts, Ji Young Lee, Chun Zhou, Xi-Ming Yang, Michael V. Cohen, James M. Downey, Robert A. Barrington, Domenico Spadafora, Jonathon P. Audia, Dara W. Frank, Sarah Voth, Viktoriya V. Pastukh, Jessica Bell, Linn Ayers, Dhananjay T. Tambe, Amy R. Nelson, Ron Balczon, Mike T. Lin, Troy Stevens

University Faculty and Staff Publications

Pneumonia elicits the production of cytotoxic beta amyloid (Aβ) that contributes to end-organ dysfunction, yet the mechanism(s) linking infection to activation of the amyloidogenic pathway that produces cytotoxic Aβ is unknown. Here, we tested the hypothesis that gamma-secretase activating protein (GSAP), which contributes to the amyloidogenic pathway in the brain, promotes end-organ dysfunction following bacterial pneumonia. First-in-kind Gsap knockout rats were generated. Wild-type and knockout rats possessed similar body weights, organ weights, circulating blood cell counts, arterial blood gases, and cardiac indices at baseline. Intratracheal Pseudomonas aeruginosa infection caused acute lung injury and a hyperdynamic circulatory state. Whereas infection led …

Experiences Of Middle School Students With Visual Impairments Accessing Technologies In Inclusive Classrooms, Anitha Muthukumaran

Dissertations

As the educational environment is moving more towards a technology-rich system, students with visual impairments (VI) educated in general education classrooms must be guaranteed equitable access to content curricula. The purpose of this study was to understand the experiences of middle school students with VI when accessing and using technologies in general education classrooms. In this multiple case study, three middle school students with VI were observed in general education settings for two school days. In addition to the students, general education teachers and teachers of students with VI (TVI) also participated in the study to understand how best they …

A Bayesian Approach To Unit Lindley Mixed Model, Nirajan Bam

Dissertations

In the applied filed of many areas such as Business and Economics, Physical, Biological, Medical, Environmental, and Social Sciences, Psychology, and Educations outcome measurements like rates, proportions, and fractions are common. Researchers have proven that using normal linear regression to analyze the relationship between outcome measurements such as rates and proportions and a set of independent variables can violates key assumptions of the method. Transformation techniques are usually applied to correct the assumptions, but this practice can violate the probability property of bounded nature of outcome variables. Even though beta regression was one of the popular methods to analyze bounded …

Asparagine Restriction Enhances Cd8+ T Cell Metabolic Fitness And Antitumoral Functionality Through An Nrf2-Dependent Stress Response, J. N. Rashida Gnanaprakasam, Bhavana Kushwaha, Lingling Liu, Xuyong Chen, Siwen Kang, Tingting Wang, Teresa A. Cassel, Christopher M. Adams, Richard M. Higashi, David A. Scott, Gang Xin, Zihai Li, Jun Yang, Andrew N. Lane, Teresa Whei-Mei Fan, Ji Zhang, Ruoning Wang

Markey Cancer Center Faculty Publications

Robust and effective T cell immune surveillance and cancer immunotherapy require proper allocation of metabolic resources to sustain energetically costly processes, including growth and cytokine production. Here, we show that asparagine (Asn) restriction on CD8+ T cells exerted opposing effects during activation (early phase) and differentiation (late phase) following T cell activation. Asn restriction suppressed activation and cell cycle entry in the early phase while rapidly engaging the nuclear factor erythroid 2-related factor 2 (NRF2)-dependent stress response, conferring robust proliferation and effector function on CD8+ T cells during differentiation. Mechanistically, NRF2 activation in CD8+ T cells conferred by Asn restriction …

Chemokine Receptor Cxcr7 Activates Aurora Kinase A And Promotes Neuroendocrine Prostate Cancer Growth, Galina Gritsina, Ka-Wing Fong, Xiaodong Lu, Zhuoyuan Lin, Wanqing Xie, Shivani Agarwal, Dong Lin, Gary E. Schiltz, Himisha Beltran, Eva Corey, Colm Morrissey, Yuzhuo Wang, Jonathan C. Zhao, Maha Hussain, Jindan Yu

Markey Cancer Center Faculty Publications

CXCR7 is an atypical chemokine receptor that recruits β-arrestin (ARRB2) and internalizes into clathrin-coated intracellular vesicles where the complex acts as a scaffold for cytoplasmic kinase assembly and signal transduction. Here, we report that CXCR7 was elevated in the majority of prostate cancer (PCa) cases with neuroendocrine features (NEPC). CXCR7 markedly induced mitotic spindle and cell cycle gene expression. Mechanistically, we identified Aurora Kinase A (AURKA), a key regulator of mitosis, as a novel target that was bound and activated by the CXCR7-ARRB2 complex. CXCR7 interacted with proteins associated with microtubules and golgi, and, as such, the CXCR7-ARRB2-containing vesicles trafficked …

Co-Targeting Nucleus Accumbens Associate 1 And Nf-Κb Signaling Synergistically Inhibits Melanoma Growth, Lixiang Gu, Xingcong Ren, Chrispus Ngule, Xiaofang Xiong, Jianxun Song, Zhiguo Li, Jin-Ming Yang

Markey Cancer Center Faculty Publications

Nucleus-accumbens-associated protein-1 (NAC1) is a cancer-related transcriptional factor encoded by the NACC1 gene, which is amplified and overexpressed in various human cancers and has been appreciated as one of the top potential cancer driver genes. NAC1 has therefore been explored as a potential therapeutic target for managing malignant tumors. Here, we show that NAC1 is a negative regulator of NF-κB signaling, and NAC1 depletion enhances the level of the nuclear NF-κB in human melanoma. Furthermore, the inhibition of NF-κB signaling significantly potentiates the antineoplastic activity of the NAC1 inhibition in both the cultured melanoma cells and xenograft tumors. This study …

Identification Of Immunodominant T Cell Epitopes Induced By Natural Zika Virus Infection, Christopher S. Eickhoff, Krystal A. Meza, Frances E. Terry, Chase G. Colbert, Azra Blazevic, Andres H. Gutiérrez, E. Taylor Stone, James D. Brien, Amelia K. Pinto, Hana M. El Sahly, Mark J. Mulligan, Nadine Rouphael, Maria L. Alcaide, Kay M. Tomashek, Chris Focht, William D. Martin, Leonard Moise, Anne S. De Groot, Daniel F. Hoft

Markey Cancer Center Faculty Publications

Zika virus (ZIKV) is a flavivirus primarily transmitted by Aedes species mosquitoes, first discovered in Africa in 1947, that disseminated through Southeast Asia and the Pacific Islands in the 2000s. The first ZIKV infections in the Americas were identified in 2014, and infections exploded through populations in Brazil and other countries in 2015/16. ZIKV infection during pregnancy can cause severe brain and eye defects in offspring, and infection in adults has been associated with higher risks of Guillain-Barré syndrome. We initiated a study to describe the natural history of Zika (the disease) and the immune response to infection, for which …

Identification Of Monotonically Expressed Long Non-Coding Rna Signatures For Breast Cancer Using Variational Autoencoders, Dongjiao Wang, Ling Gao, Xinliang Gao, Chi Wang, Suyan Tian

Markey Cancer Center Faculty Publications

As breast cancer is a multistage progression disease resulting from a genetic sequence of mutations, understanding the genes whose expression values increase or decrease monotonically across pathologic stages can provide insightful clues about how breast cancer initiates and advances. Utilizing variational autoencoder (VAE) networks in conjunction with traditional statistical testing, we successfully ascertain long non-coding RNAs (lncRNAs) that exhibit monotonically differential expression values in breast cancer. Subsequently, we validate that the identified lncRNAs really present monotonically changed patterns. The proposed procedure identified 248 monotonically decreasing expressed and 115 increasing expressed lncRNAs. They correspond to a total of 65 and 33 …

Metabolic Disruption Induced By Mtor Signaling Pathway Inhibition In Regulatory T-Cell Expansion For Clinical Application, Roberto Gedaly, Gabriel Orozco, Alexandre P. Ancheta, Mackenzie Donoho, Siddharth Desai, Fanny Chapelin, Aman Khurana, Lillie J. Lewis, Cuiping Zhang, Francesc Marti

Markey Cancer Center Faculty Publications

Background: Regulatory T cell (Treg) therapy is considered an alternative approach to induce tolerance in transplantation. If successful, this therapy may have implications on immunosuppression minimization/withdrawal to reduce drug-induced toxicity in patients. The aim of this study was to assess the efficacy of the mTORC1/C2 inhibitor, AZD8055, in the manufacturing of clinically competent Treg cells and compare the effects with those induced by rapamycin (RAPA), another mTOR inhibitor commonly used in Treg expansion protocols.

Methods: Primary human Treg cells were isolated from leukapheresis product. Cell viability, expansion rates, suppressive function, autophagy, mitochondrial unfolded protein response (mitoUPR), and cell metabolic profile …

Phosphatase And Pseudo-Phosphatase Functions Of Phosphatase Of Regenerating Liver 3 (Prl-3) Are Insensitive To Divalent Metals In Vitro, Jeffery T. Jolly, Ty C. Cheatham, Jessica S. Blackburn

Markey Cancer Center Faculty Publications

Phosphatase of regenerating liver 3 (PRL-3) is associated with cancer metastasis and has been shown to interact with the cyclin and CBS domain divalent metal cation transport mediator (CNNM) family of proteins to regulate the intracellular concentration of magnesium and other divalent metals. Despite PRL-3’s importance in cancer, factors that regulate PRL-3’s phosphatase activity and its interactions with CNNM proteins remain unknown. Here, we show that divalent metal ions, including magnesium, calcium, and manganese, have no impact on PRL-3’s structure, stability, phosphatase activity, or CNNM binding capacity, indicating that PRL-3 does not act as a metal sensor, despite its interaction …

Portable Optical Spectroscopic Assay For Non-Destructive Measurement Of Key Metabolic Parameters On In Vitro Cancer Cells And Organotypic Fresh Tumor Slices, Jing Yan, Carlos Frederico Lima Goncalves, Madison O. Korfhage, Zahid Hasan, Teresa Whei-Mei Fan, Xiaoqin Wang, Caigang Zhu

Markey Cancer Center Faculty Publications

To enable non-destructive metabolic characterizations on in vitro cancer cells and organotypic tumor models for therapeutic studies in an easy-to-access way, we report a highly portable optical spectroscopic assay for simultaneous measurement of glucose uptake and mitochondrial function on various cancer models with high sensitivity. Well-established breast cancer cell lines (MCF-7 and MDA-MB-231) were used to validate the optical spectroscopic assay for metabolic characterizations, while fresh tumor samples harvested from both animals and human cancer patients were used to test the feasibility of our optical metabolic assay for non-destructive measurement of key metabolic parameters on organotypic tumor slices. Our optical …

Structures Of The Insecticidal Toxin Complex Subunit Xpta2 Highlight Roles For Flexible Domains, Cole L. Martin, David W. Chester, Christopher D. Radka, Lurong Pan, Zhengrong Yang, Rachel C. Hart, Elad M. Binshtein, Zhao Wang, Lisa Nagy, Lawrence J. Delucas, Stephen G. Aller

Markey Cancer Center Faculty Publications

The Toxin Complex (Tc) superfamily consists of toxin translocases that contribute to the targeting, delivery, and cytotoxicity of certain pathogenic Gram-negative bacteria. Membrane receptor targeting is driven by the A-subunit (TcA), which comprises IgG-like receptor binding domains (RBDs) at the surface. To better understand XptA2, an insect specific TcA secreted by the symbiont X. nematophilus from the intestine of entomopathogenic nematodes, we determined structures by X-ray crystallography and cryo-EM. Contrary to a previous report, XptA2 is pentameric. RBD-B exhibits an indentation from crystal packing that indicates loose association with the shell and a hotspot for possible receptor binding or a …

The Role Of Vaccination Route With An Adenovirus-Vectored Vaccine In Protection, Viral Control, And Transmission In The Sars-Cov-2/K18-Hace2 Mouse Infection Model, Alexandria Dickson, Elizabeth Geerling, E. Taylor Stone, Mariah Hassert, Tara L. Steffen, Taneesh Makkena, Madeleine Smither, Katherine E. Schwetye, Jianfeng Zhang, Bertrand Georges, M. Scot Roberts, John J. Suschak, Amelia K. Pinto, James D. Brien

Markey Cancer Center Faculty Publications

Introduction: Vaccination is the most effective mechanism to prevent severe COVID-19. However, breakthrough infections and subsequent transmission of SARS-CoV-2 remain a significant problem. Intranasal vaccination has the potential to be more effective in preventing disease and limiting transmission between individuals as it induces potent responses at mucosal sites.

Methods: Utilizing a replication-deficient adenovirus serotype 5-vectored vaccine expressing the SARS-CoV-2 RBD (AdCOVID) in homozygous and heterozygous transgenic K18-hACE2, we investigated the impact of the route of administration on vaccine immunogenicity, SARS-CoV-2 transmission, and survival.

Results: Mice vaccinated with AdCOVID via the intramuscular or intranasal route and subsequently challenged with SARS-CoV-2 showed …

The S1p Receptor 1 Antagonist Ponesimod Reduces Tlr4-Induced Neuroinflammation And Increases Aβ Clearance In 5xfad Mice, Zhihui Zhu, Liping Zhang, Ahmed Elsherbini, Simone M. Crivelli, Priyanka Tripathi, Carmen Harper, Zainuddin Quadri, Stefka D. Spassieva, Erhard Bieberich

Markey Cancer Center Faculty Publications

Background Previously, we showed that the sphingosine-1-phosphate (S1P) transporter spinster 2 (Spns2) mediates activation of microglia in response to amyloid β peptide (Aβ). Here, we investigated if Ponesimod, a functional S1P receptor 1 (S1PR1) antagonist, prevents Aβ-induced activation of glial cells and Alzheimer’s disease (AD) pathology.

Methods We used primary cultures of glial cells and the 5XFAD mouse model to determine the effect of Aβ and Ponesimod on glial activation, Aβ phagocytosis, cytokine levels and pro-inflammatory signaling pathways, AD pathology, and cognitive performance.

Findings Aβ42 increased the levels of TLR4 and S1PR1, leading to their complex formation. Ponesimod prevented the …

Weighted Mean Difference Statistics For Paired Data In The Presence Of Missing Values, Yuntong Li, Brent J. Shelton, William St Clair, Heidi L. Weiss, John L. Villano, Arnold Stromberg, Chi Wang, Li Chen

Markey Cancer Center Faculty Publications

Missing data is a common issue in many biomedical studies. Under a paired design, some subjects may have missing values in either one or both of the conditions due to loss of follow-up, insufficient biological samples, etc. Such partially paired data complicate statistical comparison of the distribution of the variable of interest between the two conditions. In this article, we propose a general class of test statistics based on the difference in weighted sample means without imposing any distributional or model assumption. An optimal weight is derived from this class of tests. Simulation studies show that our proposed test with …

Toward Nonparametric Propensity Score Estimation With Guaranteed Covariate Balance, Yan Li

Dissertations and Theses (Open Access)

Establishing clear causality between exposures and outcomes is often complicated by confounders in observational studies, which leads to imbalance in covariate distributions between treatments and biased treatment effect inference. The propensity score (PS) has been widely used to adjust this covariate imbalance in observational data. However, the propensity score analysis methods rely on a correctly specified parametric PS model. When the model is misspecified, the covariate imbalance may occur, which leads to biased estimation of the treatment effect. Therefore, it is necessary to study how to improve the model misspecification in propensity score analysis.

My Ph.D. dissertation consists of three …

Helping Your Child Manage Anxiety: A Parent Education Workshop, Clarissa Aglaén Gallardo

Electronic Theses, Projects, and Dissertations

Anxiety has become one of the most common mental health disorders in young children, and it has been shown to have a significant impact on their development. Anxiety can make a child more susceptible to a number of negative effects, including a weakened immune system, developing “negative” habits such as nail biting, increased proneness to anger or crying, as well as difficulties in learning and retaining information. Conversely, when parents/caregivers can prevent or decrease anxiety in their children, children can better regulate their emotions, have more successful relationships, take more risks, and cope better with challenges. The purpose of this …

Utilizing Crispr Cas9 To Visualize Dopamine Receptors In Caenorhabditis Elegans, Lauren Michelle Velasquez

Electronic Theses, Projects, and Dissertations

Dopamine (DA) is a neurotransmitter with imperative implications in many functions including movement, reward, and cognition. Studying the pathways of dopaminergic neurons at multiple levels allows us to understand the ways in which these systems can go wrong. We study dopamine in a model system such as the worm Caenorhabditis elegans because of its relatively simple and well-characterized nervous system. DA is involved in regulating chemosensory behaviors in worms. The purpose of this research project is to definitively answer the following question: Are the dopamine receptors DOP-1 and DOP-4 expressed in chemosensory neurons? Previous reporter assays show that neither of …

The Impact Of Infant-Toddler Screen Time On Self-Regulation And Behavior Challenges: The Role Of Parenting Style And Socio-Economic Status, Tara O'Malley

Electronic Theses, Projects, and Dissertations

As young children are growing up in a digitally enmeshed world, there is growing concern about the developmental impacts of screen time. Given the importance of early rapid brain development, understanding the developmental outcomes of screen time in early childhood is critical. Current research suggests that excessive screen time may lead to delays in physical, cognitive, and language development. However, the impact of screen time on socio-emotional development among young children (0-5 years) is not well understood. Therefore, the present study examined the effects of screen time on social-emotional outcomes of 12-to-36-month-old children, specifically, the impact on self-regulation and behavior …

Mapping The Binding Site Within Integrin D2 For Carboxyethylpyrrole (Cep)-Modified Proteins, Afia Prema

Electronic Theses and Dissertations

Neutrophils and macrophages accumulate at sites of inflammation and cause chronic inflammation leading to various diseases. Therefore, to better understand chronic disease pathways it is important to investigate the properties of macrophage accumulation in inflamed tissues. The I-domain of the macrophage receptor integrin a_Db₂ plays a vital role in macrophage retention by binding to CEP (carboxyethyl pyrrole), a ligand available at inflammatory sites. This thesis mainly focuses on evaluating the binding site within integrin a_Db₂ that binds carboxyethyl pyrrole (CEP)-modified proteins. So, a recombinant plasmid construct containing the integrin I-domain was developed. Seven non-conserved …

Sound And Music Opportunities In Toddler Learning Environments, Sonia Akiko Yoshizawa

Electronic Theses and Dissertations

The aim of this qualitative research was to gain an in-depth understanding of how teachers of toddlers (aged 25-36 months) described sound and music learning opportunities and experiences provided to children in their learning environments. In this research, teachers were asked how their personal and professional experiences with sound and music influenced their planning of toddlers’ sound and music learning opportunities. The design of this study was in-depth interviews of six toddler lead teachers in three childcare sites. Data were gathered through photographs, planning documents, and in-depth interviews using a university IRB-approved video conferencing program. Findings showed that all participants …

Impacts Of Urbanization On Pollination Success For Common Milkweed (Asclepias Syriaca), David Rockow

Electronic Theses and Dissertations

Urbanization is among the greatest threats to biodiversity on the planet, and is projected to increase in magnitude in coming years. This growing threat makes it important to better understand how urbanization may impact vital ecosystem services, such as pollination. Studies on the impact of urbanization on pollination vary wildly in their findings. This study analyzed multiple aspects of pollination success in common milkweed populations varying in urbanization level, including insect community composition, pollen removal/deposition, and fruit/seed production. By analyzing multiple pollination aspects we can determine the overall mechanism in which urbanization instills on the entire pollination process. Overall, urbanization …

Behavioral Indicators Of Reflective Functioning In Mother-Child Dyadic Interactions, Rachel Clingensmith

Electronic Theses and Dissertations

Positive parenting practices and secure attachments are consistently linked to healthy child outcomes (Ainsworth & Bowlby, 1991; Waters et al., 2000). Research on cognitive processes that scaffold parental behaviors which contribute to secure attachment is an essential contribution to the literature, particularly given the potential for early intervention with at-risk families. Parental Reflective Functioning (PRF) is a construct of increasing interest which has been linked to secure attachments and positive child outcomes, with one commonly used self-report measure of PRF being the Parental Reflective Functioning Questionnaire (PRFQ; Camoirano, 2017; Clingensmith, 2021; Luyten et al., 2017). As such, the purpose of …

Comparison Of The 2022 Monkeypox (Mpox) Outbreak Using Mathematical Modeling And Time Series Clustering, Mark-Daniels Tamakloe

Electronic Theses and Dissertations

Monkeypox virus (MPXV) is the causative agent of monkeypox (mpox), a rare viral disease that affects humans [1]. It is primarily found in Africa and is transmitted to humans through contact with sick animals, particularly rodents and monkeys, or through human-to-human transmission [2]. From the beginning of May 2022, cases of mpox have been recorded from non-endemic nations, and the illness has continued to be reported in other endemic nations. Majority of confirmed cases have been recorded in Europe and North America. In this thesis, we compare the spread of the outbreak across the top ten countries using a combination …

An Early Pliocene Fish Assemblage From The Southern Appalachians: Ichthyofauna Of The Gray Fossil Site, Shay Maden

Electronic Theses and Dissertations

The Gray Fossil Site (GFS) preserves an early Pliocene sinkhole pond that accumulated a rich flora and vertebrate fauna. Fossils of fish are incredibly common at GFS, representing thousands of specimens recovered to date. Comparison with extant species reveals a depauperate freshwater fish fauna consisting of only two species: Lepomis sp. and Micropterus sp. The absence of fishes that are ubiquitous in modern streams suggests no perennial surface hydrologic connection between the Gray Fossil Site and the nearby Holston River. Limited opportunities for dispersal into the pond and deleterious conditions such as eutrophy and high competition may have resulted in …

Microsporidian Spores And The Integrin Binding Loop Of The Madam Protein Are Important For Integrin Signaling And Attachment To Host Cells, Cindy L. Barrett

Electronic Theses and Dissertations

Microsporidia are a distant fungal pathogen that have severe clinical consequences for the immunocompromised. Previous work identified a microsporidian pathogen protein termed Microsporidian ADAM or MADAM. This protein has close sequence homology to other ADAM proteins (A Disintegrin and Metalloproteinase) in two microsporidian species, Encephalitozoon intestinalis and E. cuniculi. ADAM proteins have a wide range of functions, including binding to integrins and host signaling. It is known that many pathogens manipulate integrins to invade host cells, and it is predicted that microsporidia are also exploiting this host target. Previous work with the MADAM protein demonstrated that this protein has a …