Digital Commons Network^™

Elucidating Mechanisms Of Rad51 Regulation During The Replication Stress Response, Alexandra Hawks

All Dissertations

The genome must be accurately and efficiently replicated each cell cycle to ensure there is not accumulation of mutations or structural rearrangements. When an active replication fork stalls, there are multiple DNA damage tolerance (DTT) pathways that can act to both protect integrity of DNA at the fork and restart replication. RAD51, a vital recombinase, works with HLTF, ZRANB3, and SMARCAL1 or FBH1 to regress stalled replication forks. RAD51 is loaded and stabilized onto the replication fork to protect the newly synthesized DNA from degradation. RAD51 is also active during the template switching DDT pathway. This pathway can be utilized …

Identification Of Acidic Residues In Proteins By Selective Chemical Labeling And Tandem Mass Spectrometry, Fnu Ashima

Chemistry & Biochemistry Theses - Archive

Proteins play important role in carrying out a wide range of cellular activities. They are subjected to various post-translational modifications (PTMs) by the addition of functional groups such as acetyl, phosphoryl, and methyl which leads to changes in their biological functions, localization, activity, and structure. The growth of MS technology has immensely facilitated the identification of PTMs comprehensively. This dissertation focuses on mapping these changes using covalent labeling techniques by tagging carboxylic acid residues, which undergo various kinds of PTMs. The second chapter focuses on the methods designed for Affinity Purification Mass Spectrometry (AP-MS) chemical cross-linking (CXL) of protein complexes. …

Biodiversity In The Anthropocene, Understanding The Impacts Of Land-Use Change, And Species Interactions., Alexander H. Murray

Biology Dissertations - Archive

The extent of human influences on the environment and biodiversity has led to naming our current time period the Anthropocene. A primary way in which humans impact biodiversity is through habitat modification. Despite the knowledge that habitat modification is negative for many species, we still don’t understand why some species are able to persist despite habitat modification while others do not. In my first chapter I used a trait-based approach to understand what determines species’ sensitivity to habitat modification. Trait-based approaches from different regions often disagree on the importance of the same traits, and I thought climate and land use …

Determining How Anthropogenic Change Filters Biological Communities And How Species Modify Their Traits In Response To Urbanization, Kristopher Warren Row

Biology Dissertations - Archive

Humans are significantly altering the environment at an alarming pace, leading to a reduction in species diversity. This transformation has created modified habitats where some species struggle to survive, while a few others not only manage to survive but thrive. One of the most drastic forms of habitat modification is urbanization, which is spreading worldwide and contributing to the decline of biodiversity. Urbanization has given rise to urban-tolerant species that differ ecologically from species that avoid urban areas across various niche dimensions. These urban-tolerant species must adapt to changes in food sources, microhabitat conditions, and alterations in physical habitat structures. …

“My Mother Hates Her Body, She Swears She Loves Mine”: The Effects Of Socialization Through Mothering On Daughters’ Body Talk And Intuitive Eating., Andrea N. Jenkins

Sociology & Anthropology Theses - Archive

This study examines the effects of early socialization about the body within mother/daughter relationships and its impact on the practice of Intuitive Eating (a practice of listening to one’s own body regarding food, rather than following dieting rules). Though researchers have widely explored mother/daughter relationships and dieting, this study seeks to build on this previous research, while also examining it through a new lens of adult women’s experiences with Intuitive Eating. In-depth interviews were conducted with 15 adult women who identify as Intuitive Eaters. Interviews focused on answering three research questions; (1) how does early socialization about food and the …

Modulation Of Voltage-Gating And Hysteresis Of Lysenin Channels By Cu2+ Ions, Andrew Bogard, Pangaea W. Finn, Aviana R. Smith, Ilinca M. Flacau, Rose Whiting, Daniel Fologea

Physics Faculty Publications and Presentations

The intricate voltage regulation presented by lysenin channels reconstituted in artificial lipid membranes leads to a strong hysteresis in conductance, bistability, and memory. Prior investigations on lysenin channels indicate that the hysteresis is modulated by multivalent cations which are also capable of eliciting single-step conformational changes and transitions to stable closed or sub-conducting states. However, the influence on voltage regulation of Cu²⁺ ions, capable of completely closing the lysenin channels in a two-step process, was not sufficiently addressed. In this respect, we employed electrophysiology approaches to investigate the response of lysenin channels to variable voltage stimuli in the presence …

Social Networks And Instructional Reform In Stem: The Teaching-Research Nexus, Katherine Kappelman, John P. Ziker, Karl Mertens, Brittnee Earl, Susan E. Shadle

Anthropology Faculty Publications and Presentations

Instructional reform in STEM aims for the widespread adoption of evidence based instructional practices (EBIPS), practices that implement active learning. Research recognizes that faculty social networks regarding discussion or advice about teaching may matter to such efforts. But teaching is not the only priority for university faculty – meeting research expectations is at least as important and, often, more consequential for tenure and promotion decisions. We see value in understanding how research networks, based on discussion and advice about research matters, relate to teaching networks to see if and how such networks could advance instructional reform efforts. Our research examines …

The Antiviral Effects Of A Mek1/2 Inhibitor Promote Tumor Regression In A Preclinical Model Of Human Papillomavirus Infection-Induced Tumorigenesis, Adrian J. Luna, Jesse M. Young, Rosa T. Sterk, Virginie Bondu, Fred A. Schultz, Donna F. Kusewitt, Huining Kang, Michelle A. Ozbun

Pathology Research and Scholarship

Human papillomaviruses (HPVs) are a significant public health concern due to their widespread transmission, morbidity, and oncogenic potential. Despite efficacious vaccines, millions of unvaccinated individuals and those with existing infections will develop HPV-related diseases for the next two decades and beyond. The continuing burden of HPV-related diseases is exacerbated by the lack of effective therapies or cures for infections, highlighting the need to identify and develop antivirals. The experimental murine papillomavirus type 1 (MmuPV1) model provides opportunities to study papillomavirus pathogenesis in cutaneous epithelium, the oral cavity, and the anogenital tract. However, to date the MmuPV1 infection model has not …

Association Of Amnioinfusion Volume At The Time Of Surgery For Twin-Twin Transfusion Syndrome And Latency To Delivery., Braxton Forde, Foong-Yen Lim, David N Mckinney, Mounira Habli, Kara B Markham, Mallory Hoffman, Sammy Tabbah, Marc Oria, Jose L Peiro

Women’s Health Articles

OBJECTIVE: To evaluate the impact of amnioinfusion and other peri-operative factors on pregnancy outcomes in the setting of Twin-twin transfusion syndrome (TTTS) treated via fetoscopic laser photocoagulation (FLP).

METHODS: Retrospective study of TTTS treated via FLP from 2010 to 2019. Pregnancies were grouped by amnioinfusion volume during FLP (< 1 L vs. ≥1 L). The primary outcome was latency from surgery to delivery. An amnioinfusion statistic (AIstat) was created for each surgery based on the volume of fluid infused and removed and the preoperative deepest vertical pocket. Regression analysis was planned to assess the association of AIstat with latency.

RESULTS: Patients with amnioinfusion of ≥1 L at the time of FLP had decreased latency from surgery to delivery (61 ± 29.4 vs. 73 ± 28.8 days with amnioinfusion < 1 L, p < 0.001) and increased preterm prelabor rupture of membranes (PPROM) < 34 weeks (44.7% vs. 33.5%, p = 0.042). Amnioinfusion ≥1 L was associated with an increased risk of delivery < 32 weeks (aRR 2.6, 95% CI 1.5-4.5), 30 weeks (aRR 2.4, 95% CI 1.5-3.8), and 28 weeks (aRR 1.9, 95% CI 1.1-2.3). Cox-proportional regression revealed that AIstat was inversely associated with latency (HR 1.1, 95% CI 1.1-1.2).

CONCLUSION: Amnioinfusion ≥1 L during FLP was associated with decreased latency after surgery and increased PPROM < 34 weeks.

The Applicability Of Lessons From American Society For The European Union: Tolerance, Demographic Change, And Social Structure, Joseph Drew

Comparative Civilizations Review

While Americans have long tended to see our society and civilization as exceptional, in fact the civilization is primarily based on European precedent. We see ourselves, in the words of one of our most significant poets, Emma Lazarus, as the new colossus, “Keep, ancient lands, your storied pomp cries she... I lift my lamp beside the golden door.” We claim to be an exception; that means that we are unique, different, what many, alluding to the New Testament as viewed by the Pilgrim founding fathers, have called “a shining city upon a hill.”2 And as “a beacon of hope.”

Prevention And Reversal Of Elastin-Associated And Phosphate-Dependent Arterial Calcification Using Targeted Nanotherapeutics In Pre-Clinical Models, Fatema Tuj Zohora

All Dissertations

Vascular calcification can be life-threatening, involving calcium phosphate crystal deposits in the blood vessels. The calcification in the intimal layer of the arterial wall is usually associated with atherosclerotic plaque build-up that, in the advanced stage, harbors ‘dispersed’ and ‘spotty’ deposits of calcium phosphate crystals in the necrotic core of the plaque. In contrast, medial arterial calcification (MAC) can form independently of atherosclerosis and appears as ‘Railroad-like’ calcific deposits along degraded elastin lamellae in the medial layer of arteries. The mechanisms of such elastin-associated medial arterial calcification and potential therapies are the key concerns of this dissertation. MAC is common …

Song Structure Of Male Northern House Wrens And Patterns Of Song Production And Delivery Across The Nesting Cycle, Rachael A. Disciullo, Scott K. Sakaluk, Charles Thompson

Faculty Publications – Biological Sciences

Studies of the role of bird song in avian communication have revealed numerous functions across many species, including its critical importance in female mate choice (intersexual selection) and male-male competition (intrasexual selection). A first step in understanding the role of song in different contexts is to describe the note, syllable, and song-types that occur, as well as their patterns of production within a population. Here, we do so for a north-central Illinois, USA, population of Northern House Wrens (Troglodytes aedon). As has been described in other populations, most songs (≈ 1–3 s in duration) contain an introductory section of soft, …

Essays On Human Capital, Labor, And Gender, Kritika Sen Chakraborty

Economics ETDs

This dissertation is a collection of chapters that demonstrate the factors affecting human capital and labor market outcomes of adolescents and the gender differences in the quality of employment of adults in South Africa.

The second chapter evaluates the impact of South Africa’s Child Support Grant, an unconditional means-tested grant provided to age-eligible children, on adolescent nutrition outcomes. The findings suggest that welfare programs can alleviate extreme undernutrition and overnutrition among females during the critical growth period of adolescence.

In the third chapter, I use longitudinal data from rural South Africa linked with geospatial climate indicators to examine the effect …

Mediumship And Mental Health: Investigating Spirit Communication And The Importance Of Integration, Daniel A. Seda Ph.D.

Journal of Conscious Evolution

Sensitivity to paranormal phenomena can be a source of emotional, mental and psychospiritual stress for individuals demonstrating verified mediumistic abilities, yet integrating these anomalous experiences in a healthy, affirming manner can reduce distress and potentially deflect egodystonic diagnoses of pathology. Because mediums can relay information which appear to be otherworldly from meditation, during trance, or by automatic writing and painting, health professionals have diagnosed mediums with mental disorders such as dissociative identity disorder and schizophrenia. This overreliance on pathology to explain anomalous experiences is troublesome and can cause severe distress to individuals demonstrating extrasensory capabilities. This paper was written to …

Distinguishing Servant Leadership From Transactional And Transformational Leadership, Seth-Aaron Martinez, Nahari Leija

Organizational Performance and Workplace Learning Faculty Publications and Presentations

Problem

The scholarship of leadership abounds with the affordances, limitations, antecedents, and outcomes associated with the different theories in Human Resource Development (HRD) literature. However, a clear delineation between the host of individual leadership theories does not exist. Absent is a nuanced view of the similarities, differences, and any overlap between the various leadership theories. Without a clear understanding of the relationships between leadership theories, knowing when to apply which theories and when becomes difficult.

Solution

A systematic review of the literature surrounding servant leadership (SL) through 2022 was conducted to position SL among the more extensively researched transactional and …

Racial Disparities In Access To Dbs: Results Of A Real-World U.S. Claims Data Analysis, Michael Frassica, Drew S Kern, Mitra Afshari, Allison T Connolly, Chengyuan Wu, Nathan Rowland, Juan Ramirez-Castaneda, Mwiza Ushe, Claudia Salazar, Xenos Mason

Farber Institute for Neuroscience Faculty Papers

INTRODUCTION: Deep brain stimulation (DBS) is an effective and standard-of-care therapy for Parkinson's Disease and other movement disorders when symptoms are inadequately controlled with conventional medications. It requires expert care for patient selection, surgical targeting, and therapy titration. Despite the known benefits, racial/ethnic disparities in access have been reported. Technological advancements with smartphone-enabled devices may influence racial disparities. Real-world evidence investigations can shed further light on barriers to access and demographic disparities for DBS patients.

METHODS: A retrospective cross-sectional study was performed using Medicare claims linked with manufacturer patient data tracking to analyze 3,869 patients who received DBS. Patients were …

Pulmonary & Critical Care Insider: Issue 1, Bharat Bajantri Md, Sarah Ellsworth Mls

Critical Care & Pulmonary

Pulmonary & Critical Care Insider Issue 1

Compiled by Bharat Bajantri, MD, and librarian Sarah Ellsworth, MLS for the clinicians of the Pulmonary and Critical Care team of Parkview.

The goal of this newsletter is to give a brief summary of the most pertinent articles to everyday practice and an attempt to keep up with literature as best as possible. Newsletters go out every 1-2 months.

Summaries/Topics in this issue:

• Comparison of two commonly prescribed regimens of empiric antibiotics.
• Vancomycin and piperacillin-tazobactam (VPT)
• The efficacy and safety of Dupilumab in patients with COPD
• The BOREAS trial
• Simple aspiration versus chest …

Addressing Challenges & Reactions To Covid-19 In Academic Libraries, Teisha Robertson, Edna Foxhall, Monica Hodge

Alabama Libraries

The unprecedented COVID-19 crisis has impacted many universities and academic libraries resulting in normal operations ceasing temporarily. Overall, academic libraries at the start of the COVID-19 pandemic immediately adjusted to the so-called new normal and accommodated disrupted work operations to operate successfully. This article discusses the procedures implemented, positive and negative reactions to these changes, and the challenges experienced at Alabama State University, Levi Watkins Learning Center, and other libraries. This article will also highlight implemented procedures carried out by other university libraries, including difficulties and successes that libraries experienced during peak periods of the COVID-19 pandemic. To conclude, academic …

Development Of Mass Transfer Coefficient Correlations Between Carbon Dioxide And Oxygen For Air-Lift Devices, John Walker

All Theses

An experimental method for measurement and determination of carbon dioxide volumetric mass transfer coefficients (k_La) in high pH water was developed. The goal of this research was to develop a relationship between CO₂ and O₂ volumetric mass transfer coefficients for accurate k_La values in order to meet the needs of carbon capture systems and other industries. Results included dissolved oxygen (DO) and total inorganic carbon (TIC) k_La comparisons, as well as polarographic DO (mg/L) and galvanic DO (mV) k_La correlation. CO₂ k_La estimation based on O_{2 …}

An Assessment Of Clemson Cooperative Extension Agents’ Perceptions Of Work-Related Factors Leading To Burnout, Erika Hwang

All Theses

Turnover and employee burnout are problems the Cooperative Extension System is facing nationwide, and the Clemson University Cooperative Extension System is no exception. There has been previous research done within the Extension system to identify the factors contributing to turnover and burnout, however, both remain an issue. Yet, there has been no research done to examine the Clemson University Cooperative Extension System individually. To further investigate factors contributing to burnout, this pilot study was conducted with Clemson University Clemson Extension agents using a burnout assessment that has not previously been used in Extension research. This study described Clemson University Cooperative …

The Impact Of Improper Solid Waste Management On Public Health Of African Cities. The Case Of Addis Ababa City, Ethiopia., Tesfaye Abebe Alemu

Civil Engineering Dissertations - Archive

Many African cities are generating an ever-increasing amount of waste and the effectiveness of their solid waste collection and disposal systems is currently declining. Therefore, research has been striving to develop a sustainable and integrated solid waste management system that fits the current challenges facing the local environment. The objective of this study is to analyze the impact of improper solid waste management that affects the air and water quality of the near Koshe open dump site and around the Addis Ababa City, and their adverse effect on the public health living around them. The rapid increase of population in …

Effect Of Shock Tunnel Geometry On Shockwave And Vortex Ring Formation, Propagation, And Head On Collision, Rachel L. Bauer, Emily M. Johnson, Alexander D. Douglas, Catherine E. Johnson

Mining Engineering Faculty Research & Creative Works

Vortex ring research primarily focuses on the formation from circular openings. Consequently, the role of tunnel geometry is less understood, despite there being numerous research studies using noncircular shock tunnels. This experimental study investigated shockwaves and vortex rings from different geometry shock tunnels from formation at the tunnel opening to head on collision with another similarly formed vortex ring using schlieren imaging and statistical analysis. The velocity of the incident shockwave was found to be consistent across all four shock tunnel geometries, which include circle, hexagon, square, and triangle of the same cross-sectional area. The velocity was 1.2 ± 0.007 …

Book Review: Disinformation: The Nature Of Facts And Lies In The Post-Truth Era, Kristin Peace

LOEX Quarterly

No abstract provided.

$752,000 Usda Grant Will Help Researchers Prevent Food Contamination, University Of Dayton

Blog: The Dayton Engineer

A pair of University of Dayton researchers are part of a $752,000 grant from the U.S. Department of Agriculture to help prevent Listeria and other contamination in food.

Examining The Effects Of Iron-Oxide Nanoparticles On Primary Gingival Keratinocytes For The Prevention Of Oral Disease, Jennifer C. Carranza

Dental Hygiene ETDs

Oral plaque biofilm has been identified as the primary contributor to dental conditions such as dental caries and periodontal disease that promotes a diverse and pervasive collection of microbial species to inhabit the oral cavity. Disrupting the plaque matrix early could help combat this global disease burden. This study evaluated the effect of IONPs on primary gingival keratinocytes.

Purpose: This study aimed to examine existing research regarding nanoparticles and determine if IONPs affect the PGK cell membranes with preliminary research to find the maximum lysis possible with PGK cells.

Methods: IONP core/shell syntheses were performed. Considering keratinocyte characteristics, examining data …

Pitfalls In Machine Learning-Based Assessment Of Tumor-Infiltrating Lymphocytes In Breast Cancer: A Report Of The International Immuno-Oncology Biomarker Working Group On Breast Cancer, Jeppe Thagaard, Glenn Broeckx, Chowdhury Arif Jahangir, Sara Verbandt, Rajarsi Gupta, Reena Khiroya, Khalid Abduljabbar, Gabriela Acosta Haab, Balazs Acs, Shahin Sayed

Pathology, East Africa

Abstract: The clinical significance of the tumor-immune interaction in breast cancer is now established, and tumor-infiltrating lymphocytes (TILs) have emerged as predictive and prognostic biomarkers for patients with triple-negative (estrogen receptor, progesterone receptor, and HER2-negative) breast cancer and HER2-positive breast cancer. How computational assessments of TILs might complement manual TIL assessment in trial and daily practices is currently debated. Recent efforts to use machine learning (ML) to automatically evaluate TILs have shown promising results. We review state-of-the-art approaches and identify pitfalls and challenges of automated TIL evaluation by studying the root cause of ML discordances in comparison to manual TIL …

Spatial Analyses Of Immune Cell Infiltration In Cancer: Current Methods And Future Directions: A Report Of The International Immuno-Oncology Biomarker Working Group On Breast Cancer, David Page, Chowdhury Jahangir, Sara Verbandt, Rajarsi Gupta, Jeppe Thagaard, Reena Khiroya, Zuzana Kos, Khalid Abduljabbar, Sunil Badve, Shahin Sayed

Pathology, East Africa

Abstract: Modern histologic imaging platforms coupled with machine learning methods have provided new opportunities to map the spatial distribution of immune cells in the tumor microenvironment. However, there exists no standardized method for describing or analyzing spatial immune cell data, and most reported spatial analyses are rudimentary. In this review, we provide an overview of two approaches for reporting and analyzing spatial data (raster versus vector-based). We then provide a compendium of spatial immune cell metrics that have been reported in the literature, summarizing prognostic associations in the context of a variety of cancers. We conclude by discussing two well-described …

The Mediating Role Of Security Anxiety In Internet Threat Avoidance Behavior, Xuan Wang, Yaojie Li, Hanieh Javadi Khasraghi, Cherie Trumbach

Management and Marketing Faculty Publications

In the prior technology threat avoidance literature, inconsistent results are found in examining the relationships between individuals’ perceived susceptibility, perceived severity, and perceived threat, as well as the intention to avoid security threats. One possible explanation for this paradox is lacking consideration of the role of individuals’ negative emotions while facing threats, which is rarely studied before. Therefore, the current study aims to extend the landscape of technology threat avoidance theory by exploring the role of security anxiety in individuals’ responses to security threats from the Internet. Our results demonstrate that Internet users’ risk aversion is strongly influenced by their …

Functional Nanocomposite Coatings For Use In Food Packaging, Camden N. Webb

Master's Theses

Plastics are a class of materials known for their cost and property advantages, increasing significantly in their usage worldwide. Unfortunately, these benefits come with an increasingly concerning environmental impact. A combination of inadequate disposal options and combinations of materials have led to environmental disasters that will impact generations. One of the worst areas for plastic waste is food packaging. Plastic as a material generally excels at durability and longevity, but as food packaging, it outlives its intended purpose by several orders of magnitude. This leads to plastic food packaging materials sitting in landfill or leading to the environment for hundreds …

The University Of Montana: A History Through The Lens Of Physical Culture, Pe, Health, Athletics, And Recreation 1897-2019: The Evolution Of A Department, Clarence E. Burns

Clarence E. Burns Manuscript: "The University of Montana: A History Through the Lens of Physical Culture, PE, Health, Athletics, and Recreation 1897-2019"

Dr. Burns’ manuscript chronicles how the University of Montana’s Department of Elocution and Physical Culture (1897), one of the university’s oldest programs, evolved to become the Department of Physical Education and later the Department of Health and Human Performance, a program that initially organized “gymnasium practice” and later trained physical education teachers and coaches. The program eventually became one of the most popular campus venues for candidates interested in pre-medicine, physical therapy, nursing, physician assistant certification, and other post-baccalaureate health-care training programs. The manuscript also covers associated history of the university, including changing administrative styles and philosophies, legislative and public …