Digital Commons Network^™

Somatic Cell Structural Variant Mutagenesis And Neurologic Disease, James R Lupski

Faculty, Staff and Students Publications

Detection of organismal mosaic states for variant alleles faces technical and analytical challenges, as does the association of such variant alleles with susceptibility to neurologic disease. In this issue of Cell Genomics, Maury et al.¹ reanalyze genotyping arrays of a schizophrenia cohort providing evidence for the contribution of somatic structural variant mutagenesis and rare variant alleles.

Comparison Of Near-Infrared Spectroscopy-Based Cerebral Autoregulatory Indices In Extremely Low Birth Weight Infants, Howard Chao, Sebastian Acosta, Craig Rusin, Christopher Rhee

Faculty, Staff and Students Publications

BACKGROUND: Premature infants are born with immature cerebral autoregulation function and are vulnerable to pressure passive cerebral circulation and subsequent brain injury. Measurements derived from near-infrared spectroscopy (NIRS) have enabled continuous assessment of cerebral vasoreactivity. Although NIRS has enabled a growing field of research, the lack of clear standardization in the field remains problematic. A major limitation of current literature is the absence of a comparative analysis of the different methodologies.

OBJECTIVES: To determine the relationship between NIRS-derived continuous indices of cerebral autoregulation in a cohort of extremely low birth weight (ELBW) infants.

METHODS: Premature infants of birth weight 401-1000 …

Dimethyl Sulfoxide Inhibits Bile Acid Synthesis In Healthy Mice But Does Not Protect Mice From Bile-Acid-Induced Liver Damage, Xi Chen, Huiqiao Li, Yu'e Liu, Jing Qi, Bingning Dong, Shixia Huang, Shangang Zhao, Yi Zhu

Faculty, Staff and Students Publications

Bile acids serve a vital function in lipid digestion and absorption; however, their accumulation can precipitate liver damage. In our study, we probed the effects of dimethyl sulfoxide (DMSO) on bile acid synthesis and the ensuing liver damage in mice induced by bile acids. Our findings indicate that DMSO efficaciously curbs bile acid synthesis by inhibiting key enzymes involved in the biosynthetic pathway, both in cultured primary hepatocytes and in vivo. Contrarily, we observed that DMSO treatment did not confer protection against bile-acid-induced liver damage in two distinct mouse models: one induced by a 0.1% DDC diet, leading to bile …

Butte Priority Soils Operable Unit (Bpsou) Final Insufficiently Reclaimed Sites – Field Sampling Plan (Fsp) Bres No. 84 – Mandan Park Play Area, Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Effect Of Expansion And Tumor Challenge On Chemokine Receptor Expression In Cord Blood-Derived Car-Nk Cells, Sam Rosemore, Pinghua Liu, Pinaki Banerjee, Rafet Basar, Sunil Acharya, Hind Rafei, Katy Rezvani

Summer Experience 2023

No abstract provided.

Investigating The Function Of Arginine Methylation At Two Mutated Sites Of Cbp By Carm1, Nicole Dawson, Jee Won Hwang, Margarida Albuquerque Almeida Santos

Summer Experience 2023

No abstract provided.

Imaging Spectrometer Implementation On A Small Satellite Platform For Aquatic Ecosystems Science, Corrie Smeaton, Ronald Lockwood, Michael Chrisp, Charles Bachmann, Laura Kennedy, Steve Gillmer, Linda Fuhrman, Derrick Brouhard, Jade Wang

Small Satellite Conference

The implementation of Imaging spectrometers with state-of-the-art performance on small satellites is challenging due to the size, weight, and power (SWaP) limitations. We have recently developed a compact form, the Chrisp Compact VNIR/SWIR Imaging Spectrometer (CCVIS), that facilitates their usage without sacrificing performance. The CCVIS enables a modular implementation that, combined with a freeform telescope, produces a wide field of view with high signal to noise ratio (SNR) performance. The targeted scientific application is the study of aquatic ecosystems. The imaging spectrometer is designed to address carbon sequestration in coastal margins and wetlands, kelp and seagrass studies, coral reefs, harmful …

Minutes 8-8-2023 | Safety Committee, Megan Meade

Safety Committee Minutes

No abstract provided.

Machine Learning Techniques To Predict The Air Quality Using Meteorological Data In Two Urban Areas In Sri Lanka, Lakindu Mampitiya, Namal Rathnayake, Lee P. Leon, Vishwanadham Mandala, Hazi Md. Azamathulla, Sherly Shelton, Yukinobu Hoshino, Upaka Rathnayake

Department of Earth and Atmospheric Sciences: Faculty Publications

The effect of bad air quality on human health is a well-known risk. Annual health costs have significantly been increased in many countries due to adverse air quality. Therefore, forecasting air quality-measuring parameters in highly impacted areas is essential to enhance the quality of life. Though this forecasting is usual in many countries, Sri Lanka is far behind the state-of-the-art. The country has increasingly reported adverse air quality levels with ongoing industrialization in urban areas. Therefore, this research study, for the first time, mainly focuses on forecasting the PM₁₀ values of the air quality for the two urbanized areas …

Using T He Carbon Atom Method T O Determine Mean Oxidation Number O F Organic Carbons, Pong Kau Yuen, Cheng Man Diana Lau

Faculty Publications

The notion of oxidation number acting as an electron-counting concept is crucial for balancing redox reactions, and for understanding organic and biological redox conversions. Chemical formula methods are widely used for counting oxidation numbers. There are three types of chemical formula methods. They are molecular formula method, structural formula method, and Lewis formula method. Each type has its own rules and procedures, and they are difficult for students to fully understand and remember. In addition, the capability of the molecular formula method to assign mean oxidation number of organic carbons for organic molecules or molecular ions is limited. To overcome …

Effect Of Cutting Management On The Forage Production And Quality Of Tepary Bean (Phaseolus Acutifolius A. Gray), Travis W. Witt, Brian K. Northup, Timothy G. Porch, Santos Barrera, Carlos A. Urrea

Department of Agronomy and Horticulture: Faculty Publications

Tepary bean (Phaseolus acutifolius A. Gray) is an underutilized drought tolerant annual legume, originating from the Sonoran Desert, that may be a beneficial forage/hay for beef cattle in the Southern Great Plains of the US (SGP). The SGP has erratic rainfall and periods of intermittent drought exacerbated by high summer temperatures. In 2020 and 2021, a split-plot design was used to evaluate 13 genotypes of tepary bean and a forage soybean (control) at El Reno, OK, USA to compare production of plant biomass and forage nutritive value parameters under seven harvest regimes. Genotypes were used as the main plot …

Transforming Perspectives: Identifying And Addressing Disparities In Sexual Health Education In Occupational Therapy Education Programs, Devon C. Wilson

MOT Student Capstones

This capstone project aims to address the lack of sexual health education in Occupational Therapy (OT) curricula and improve OT practitioners' ability to address sex and sexuality in 5 their practice. The research sought to understand the practices and attitudes of OT educators and educational programs in the United States concerning sexual health and sexuality within their curriculum. The study employed a survey-based approach, gathering data from a diverse group of OT educators and program directors through an online platform before and after participating in an educational presentation on the subject matter. The results revealed positive views of sex and …

Awareness, Acceptance, And Equanimity: Relationships Between Trait Mindfulness, Stress, And Blood Pressure, Gabrielle Renee Chin

Theses and Dissertations

Hypertension (HTN) is associated with stress and unhealthy emotion regulation. Mindfulness-based interventions (MBIs) are said to help address stress-related diseases like cardiovascular disease by impacting stress and emotion regulation, yet studies of MBIs on cardiovascular health show inconsistent findings. Limited research has examined the basic links between trait mindfulness and cardiovascular health, leaving the active components of MBIs in this context unclear. Therefore, the current study examined the relationship between trait mindfulness and blood pressure (BP) in individuals with pre-hypertension (pre-HTN). Latent variables representing two conceptualizations of trait mindfulness -Monitor and Accept Theory (MAT) and Equanimity- were calculated using facets …

Characterizing Host-Pathogen Interactions Between Zostera Marina And Labyrinthula Zosterae, Yaamini R. Venkataraman, Amanda Shore, Sukanya Dayal, James Sanghyun Lee, Mahsa Alidoost Salimi, Grace Crandall, Malina M. Loeher, Mark Stoops, Megan Swanger, Morgan E. Eisenlord, Kathryn L. Van Alstyne, Mark D. Fast, Colleen A. Burge, Maya L. Groner

Biology

Introduction: Seagrass meadows serve as an integral component of coastal ecosystems but are declining rapidly due to numerous anthropogenic stressors including climate change. Eelgrass wasting disease, caused by opportunistic Labyrinthula spp., is an increasing concern with rising seawater temperature. To better understand the host-pathogen interaction, we paired whole organism physiological assays with dual transcriptomic analysis of the infected host and parasite.

Methods:: Eelgrass (Zostera marina) shoots were placed in one of two temperature treatments, 11° C or 18° C, acclimated for 10 days, and exposed to a waterborne inoculation containing infectious Labyrinthula zosterae (Lz) or sterile seawater. At two- and …

Optimization Of Light Spectrum During Coral Grow-Out, Daisy N. Ponce

All HCAS Student Capstones, Theses, and Dissertations

Direct and indirect anthropogenic stressors have led to a global decline of coral populations. Coral restoration aims to mitigate this loss and facilitate reef recovery. In particular, the sexual propagation of corals ex situ allows for the production and outplant of genetically diverse coral recruits on the reef. However, optimization at ex situ coral nurseries is required to scale-up production. This project aims to investigate and develop methods that reduce the duration of grow-out ex situ by determining the optimal light spectrum under which to rear for sexual recruits of the tropical scleractinian coral species Pseudodiploria strigosa and P. …

Regulation Of Stress Responses And Membrane Morphology By Dspa In Synechocystis Sp. Pcc 6803, Rebekah Kathryn White

Theses and Dissertations

In the world of microorganisms, cyanobacteria are a uniquely skilled powerhouse. They participate in global nutrient and carbon cycling. They have the ability to photosynthesize, which allows them to use sunlight and carbon dioxide to produce cellular energy. However, photosynthesis has a lot of points were environmental stresses can interfere, increasing the chances of oxidative stress and nutrient imbalances that can amplify deleterious effects. One regulator in particular will be the target of this study. DspA, also known as Hik33, is a histidine kinase that perceives and regulates multiple overlapping stress responses. In this project, abiotic stress effects will be …

Supporting The “Virtuous Cycle” In Urban Ecosystems: How Research Can Inform Plans, Policies, And Projects That Impact Urban Resilience, Michele Romilini, Sophie S. Parker, Gregory B. Pauly, Eric M. Wood

Center for Urban Resilience Scholarship

No abstract provided.

The Role Of Phenylacetic Acid (Paa) Catabolism In Acinetobacter Physiology, Anna Josephine Hooppaw

Arts & Sciences Graduate Student Theses and Dissertations

Microorganisms are constantly challenged by stressful environments and have evolved mechanisms to adapt to or counteract these threats. The Gram-negative pathogen Acinetobacter baumannii is thought to rely on a “persist and resist” strategy that facilitates its remarkable ability to survive in a variety of harsh conditions, giving it the capacity to cause life-threatening infections. Currently, over 60% of the global A. baumannii clinical isolates are multidrug resistant (MDR), leading the World Health Organization and the Centers for Disease Control and Prevention to categorize this pathogen as a top priority for the research and development of new antimicrobial therapies. Understanding the …

Bis-Cyclic Guanidines As Antibacterial Agents, Jianfeng Cai, Peng Teng, Alekhya Nimmagadda

USF Patents

Provided are novel bis-cyclic guanidine compounds, and the use thereof for treating bacterial infection.

Ten Questions Concerning Smart And Healthy Built Environments For Older Adults, Sanjay Kumar, Samuel H. Underwood, Julie L. Masters, Natalie A. Manley, Iason Konstantzos, Josephine Lau, Ryan Haller, Lily M. Wang

Durham School of Architectural Engineering and Construction: Faculty Publications

In the past decade, the term ‘global aging’ has gained much attention among researchers, policymakers, civil societies, and governments worldwide. The continuous decline in fertility rates and increased life expectancy have resulted in an ongoing demographic transition where the share of adults aged 65 or older is increasing and outnumbering children younger than five years. In the United States and other developed countries, this rapid demographic transition is expected to continue to strain the existing health infrastructure as it becomes increasingly challenging to ensure healthy living environments for older adults. The ten questions and answers in this paper have been …

Feasibility And Value Of Genomic Profiling In Cancer Of Unknown Primary: Real-World Evidence From Prospective Profiling Study, Ryan W Huey, Aakash Tushar Shah, Honey V Reddi, Priyadarsini Dasari, James T Topham, Hyunsoo Hwang, Nishat Dhillon, Anneleis Willett, Brandon G Smaglo, Jeannelyn S Estrella, Asif Rashid, Aurelio Matamoros, Michael J Overman, Linda Choquette, Greg Omerza, Kevin Kelly, Xuemei Wang, Jonathan M Loree, Jens Rueter, Gauri R Varadhachary, Kanwal Raghav

Faculty, Staff and Student Publications

Real-world evidence regarding the value of integrating genomic profiling (GP) in managing cancer of unknown primary (CUP) is limited. We assessed this clinical utility using a prospective trial of 158 patients with CUP (October 2016-September 2019) who underwent GP using next-generation sequencing designed to identify genomic alterations (GAs). Only 61 (38.6%) patients had sufficient tissue for successful profiling. GAs were seen in 55 (90.2%) patients of which GAs with US Food and Drug Administration-approved genomically matched therapy were seen in 25 (40.9%) patients. A change in therapy was recommended and implemented (primary endpoint of the study) in 16 (10.1%) and …

Maximum Size-Density Relationships In Mixed-Species And Monospecific Stands Of The Southeastern United States, Maxwell Robert Schrimpf

Theses and Dissertations

Maximum size-density relationships (MSDR) are used to quantify differences across sites in the number of trees of a given size and species that can be supported per hectare. These relationships are important to managers who are trying to maximize basal area and wood volume. In my study, I examined MSDR across Alabama, Georgia, Louisiana, and Mississippi using US Forest Service, Forest Inventory and Analysis (FIA) data. I determined the impact of species-specific, specific gravity, functional traits, and environmental factors on MSDR using a quantile regression approach. Overall, I found that climatic factors had the greatest influence on MSDR, and that …

A Comprehensive Report Of Intrinsically Disordered Regions In Inherited Retinal Diseases, Karen E Lee, Jose S Pulido, Mariana M Da Palma, Rebecca Procopio, Robert B Hufnagel, Margaret Reynolds

2020-Current year OA Pubs

BACKGROUND/PURPOSE: A comprehensive review of the degree of disorder in all genes in the Retinal Information Network (RetNet) Database is implicated in inherited retinal diseases (IRDs). Their association with a missense variation was evaluated.

METHODS: IRD genes from RetNet were included in this study. Publicly available data on the genome aggregation database (gnomAD) were used to analyze the number of total and pathogenic missense variants. Metapredict, an accurate and high-performance predictor that reproduces consensus disorder scores, was used to calculate disorder.

MAIN OUTCOME MEASURES: The main outcome measures were percent disorder, percent pathogenicity, number of total missense variants, and percent …

Experiences Of First-Generation Students In A Large Public University In Ghana : A Phenomenological Study, Jacob Oppong Nkansah

Lingnan Theses (Doctoral degree)

In this study, first-generation students (FGS) experiences in the higher education system of Ghana were explored. Only a few studies in Africa, notably Ghana, have focused on the concept of FGS. FGS are conceptualized as learners whose parents didn’t complete or attend university and are the first in their nuclear families to seek for higher education. This research looked into FGS experiences at the University of Education Winneba, which is a large public university viewed as a haven for FGS in Ghana because of its mandate to train teachers for immediate employment. The study explored FGS motivations for pursuing higher …

Exploring The School Nurse's Knowledge, Experiences, And Role In Trauma-Informed Care: Implications For School Nurse Practice, Lesha H. Rouse

Dissertations

School nurses as the only health care professional in schools with the potential to be in daily contact with students are in a critical position to create awareness, influence action, and provide leadership in trauma-informed care (TIC). However, there is a lack of research regarding school nurse TIC knowledge, experiences, and role in school health practice. Research has asserted the pervasiveness of Adverse Childhood Experiences (ACEs) in society as well as, it's potential to cause deleterious life-long health, educational, social, and economic outcomes (Bethell et al., 2017; Felitti et al., 1998; Stevens, 2019). Exploring the school nurse's role in TIC …

Hospice Interdisciplinary Team Processes And Effectiveness, Beth A. Hale

Dissertations

The purpose of this research was to test a causal model of interdisciplinary hospice processes and effectiveness. This research examined the impact of organization and team level structure constructs (organizational culture, team complexity, and team leadership) on hospice interdisciplinary team processes and subsequent influence on perceived team effectiveness. The relationships among perceived team effectiveness, team task satisfaction, and family satisfaction with hospice care were also examined.

Terror Management And The News: An Exploration Into The Effects Of Framing On Mortality Salience, Peter Montwill

Masters Theses, 2020-current

The present study explores the link between Terror Management Theory and the use of its principles within news media. Political news media in the digital age undergoes a variety of framing effects, more specifically episodic and thematic frames of stories. To induce mortality salience, college-aged participants were presented with stories framed from the perspective of an individual’s experience or a general theme of experiences regarding the controversial pro-life topic and a non-controversial hiking topic. These stories are presented in the style of Instagram posts to mirror how college-aged people consume news media. The stories also contain wording designed to induce …

Successful Treatment Of Non-Langerhans Cell Histiocytosis With The Mek Inhibitor Trametinib: A Multicenter Analysis, Ashley Aaroe, Razelle Kurzrock, Gaurav Goyal, Aaron M Goodman, Harsh Patel, Gordon Ruan, Gary Ulaner, Jason Young, Ziyi Li, Derek Dustin, Ronald S Go, Eli L Diamond, Filip Janku

Faculty, Staff and Student Publications

Erdheim-Chester disease (ECD) and Rosai-Dorfman disease (RDD) are rare non-Langerhans cell histiocytoses (non-LCHs), for which therapeutic options are limited. MAPK pathway activation through BRAFV600E mutation or other genomic alterations is a histiocytosis hallmark and correlates with a favorable response to BRAF inhibitors and the MEK inhibitor cobimetinib. However, there has been no systematic evaluation of alternative MEK inhibitors. To assess the efficacy and safety of the MEK inhibitor trametinib, we retrospectively analyzed the outcomes of 26 adult patients (17 with ECD, 5 with ECD/RDD, 3 with RDD, and 1 with ECD/LCH) treated with orally administered trametinib at 4 major US …

The Effectiveness Of Microcurrent Neurofeedback On Depression, Anxiety, Post-Traumatic Stress Disorder, And Quality Of Life, Gloria Duke, Courtney N. Yotter, Beverly Sharifian, Gary Duke, Sandra Petersen

Nursing Faculty Publications and Presentations

Background: The world faces a mental health crisis with elevated rates of depression, anxiety, and post-traumatic stress, leaving a profound impact on daily quality of life (QOL). Current treatments show varying degrees of efficacy and carry burdensome challenges. Evidence exists for use of an innovative neurotechnology to reduce symptoms of depression, anxiety, and post-traumatic stress disorder (PTSD), but the science is lacking for use in the general population. Purposes: The purpose of this pilot study was to explore the effects of microcurrent neurofeedback on depression, anxiety, PTSD symptoms, and QOL in adults. Methodology: This was a one-group, exploratory pilot study …

Phylogenetic Inference From Single-Cell Rna-Seq Data, Xuan Liu, Jason I Griffiths, Isaac Bishara, Jiayi Liu, Andrea H Bild, Jeffrey T Chang

Faculty, Staff and Student Publications

Tumors are comprised of subpopulations of cancer cells that harbor distinct genetic profiles and phenotypes that evolve over time and during treatment. By reconstructing the course of cancer evolution, we can understand the acquisition of the malignant properties that drive tumor progression. Unfortunately, recovering the evolutionary relationships of individual cancer cells linked to their phenotypes remains a difficult challenge. To address this need, we have developed PhylinSic, a method that reconstructs the phylogenetic relationships among cells linked to their gene expression profiles from single cell RNA-sequencing (scRNA-Seq) data. This method calls nucleotide bases using a probabilistic smoothing approach and then …