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The Concentration Of Heavy Metals In Lincoln Nebraska's Tap Water, Evan Neuhaus

Honors Program: Senior Projects (Public)

Heavy metals are commonplace in nature, and are typically found in scarce quantities in all drinking sources. However, in high enough concentrations, they can have a myriad of negative health effects. This study seeks to find the concentrations of such metals in the Lincoln water supply utilizing the resources of the Water Center at the University of Nebraska at Lincoln. To test for discrete concentrations of trace elements, a method of using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was implemented. ICP-MS uses plasma to ionize and atomize a sample which is then filtered into a series of cones into a …

Children In Economically Disadvantaged Households Have Lower Early Literacy Skills Than Their Higher-Income Peers, Michah W. Rothbart, Colleen Heflin, Gabriella Alphonso

Population Health Research Brief Series

Literacy is critical for numerous developmental outcomes and wellbeing among children. Low literacy skills in childhood can also negatively affect individuals in adulthood. Using data from nearly 300,000 kindergarten students in Virginia (2014-2017), this study finds that children in households that participate in more than one social assistance program (such as Temporary Assistance for Needy Families, the Supplemental Nutrition Assistance Programs, and Free or Reduced-Price Lunch) have lower literacy skills when they enter kindergarten than children whose households participate in fewer or no social programs.

Glut1 Is Redundant In Hypoxic And Glycolytic Nucleus Pulposus Cells Of The Intervertebral Disc, Shira N. Johnston, Elizabeth S. Silagi, Vedavathi Madhu, Duc H. Nguyen, Irving M. Shapiro, Makarand V. Risbud

Department of Orthopaedic Surgery Faculty Papers

Glycolysis is central to homeostasis of nucleus pulposus (NP) cells in the avascular intervertebral disc. Since the glucose transporter, GLUT1, is a highly enriched phenotypic marker of NP cells, we hypothesized that it is vital for the development and postnatal maintenance of the disc. Surprisingly, primary NP cells treated with 2 well-characterized GLUT1 inhibitors maintained normal rates of glycolysis and ATP production, indicating intrinsic compensatory mechanisms. We showed in vitro that NP cells mitigated GLUT1 loss by rewiring glucose import through GLUT3. Of note, we demonstrated that substrates, such as glutamine and palmitate, did not compensate for glucose restriction resulting …

Large And Stable Genome Edits At The Sorghum Alpha Kafirin Locus Result In Changes In Chromatin Accessibility And Globally Increased Expression Of Genes Encoding Lysine Enrichment, J. Preston Hurst, Abou Yobi, Aixia Li, Shirley Sato, Thomas E. Clemente, Ruthie Angelovici

Department of Agronomy and Horticulture: Faculty Publications

Introduction: Sorghum is a resilient and widely cultivated grain crop used for feed and food. However, it’s grain is deficient in lysine, an essential amino acid. This is due to the primary seed storage proteins, the alpha-kafirins, lacking lysine. It has been observed that reductions in alpha-kafirin protein results in rebalancing of the seed proteome and a corresponding increase in non-kafirin proteins which leads to an increased lysine content. However, the mechanisms underlying proteome rebalancing are unclear. This study characterizes a previously developed gene edited sorghum line, with deletions at the alpha kafirin locus.

Methods: A single consensus …

Editorial: Inter-Organ Crosstalk During Exercise In Health And Disease: Extracellular Vesicles As New Kids On The Block, Kenneth Verboven, Ivan Jose Vechetti Jr

Department of Nutrition and Health Sciences: Faculty Publications

Within exercise physiology, the study of factors potentially mediating interorgan crosstalk during and after exercise is a fascinating field of research. As exercise activates a plethora of metabolic pathways in several tissues, organs and systems, examining the underlying biological mechanisms contributing to exercise related metabolic benefits is imperative. Since two decades, the skeletal muscle is known to secrete humoral factors into the circulation in response to exercise, originally described as “myokines” by Pedersen et al. (2003). These myokines are now well known and extensively studied in the field of exercise science (Pedersen and Febbraio, 2012). Interestingly, exercise also triggers other …

Use Of Dual Genomic Sequencing To Screen Mitochondrial Diseases In Pediatrics: A Retrospective Analysis, Teng-Hui Wu, Jing Peng, Li Yang, Yan-Hui Chen, Xiu-Lan Lu, Jiao-Tian Huang, Jie-Yu You, Wen-Xian Ou-Yang, Yue-Yu Sun, Yi-Nan Xue, Xiao Mao, Hui-Ming Yan, Rong-Na Ren, Jing Xie, Zhi-Heng Chen, Victor-Wei Zhang, Gui-Zhen Lyu, Fang He

Children’s Nutrition Research Center Staff Publications

Mitochondrial diseases (MDs) were a large group multisystem disorders, attributable in part to the dual genomic control. The advent of massively sequencing has improved diagnostic rates and speed, and was increasingly being used as a first-line diagnostic test. Paediatric patients (aged <  18 years) who underwent dual genomic sequencing were enrolled in this retrospective multicentre study. We evaluated the mitochondrial disease criteria (MDC) and molecular diagnostic yield of dual genomic sequencing. Causative variants were identified in 177 out of 503 (35.2%) patients using dual genomic sequencing. Forty-six patients (9.1%) had mitochondria-related variants, including 25 patients with nuclear DNA (nDNA) variants, 15 with mitochondrial DNA (mtDNA) variants, and six with dual genomic variants (MT-ND6 and POLG; MT-ND5 and RARS2; MT-TL1 and NARS2; MT-CO2 and NDUFS1; MT-CYB and SMARCA2; and CHRNA4 and MT-CO3). Based on the MDC, 15.2% of the patients with mitochondria-related variants were classified as "unlikely to have mitochondrial disorder". Moreover, 4.5% of the patients with non-mitochondria-related variants and 1.43% with negative genetic tests, were classified as "probably having mitochondrial disorder". Dual genomic sequencing in suspected MDs provided a more comprehensive and accurate diagnosis for pediatric patients, especially for patients with dual genomic variants.

Renal Cell Carcinoma Unclassified With Medullary Phenotype In A Patient With Neurofibromatosis Type 2, Sanila Sarkar, Whitney Throckmorton, Racheal Bingham, Pavlos Msaouel, Giannicola Genovese, John Slopis, Priya Rao, Zsila Sadighi, Cynthia E Herzog

Faculty, Staff and Student Publications

We present, to our knowledge, the first reported case of germline neurofibromatosis Type 2 (NF2) associated with renal cell carcinoma unclassified with medullary phenotype (RCCU-MP) with somatic loss by immunohistochemistry of the SMARCB1 tumor suppressor gene located centromeric to NF2 on chromosome 22q. Our patient is a 15-year-old with germline neurofibromatosis Type 2 (NF2) confirmed by pathogenic mutation of c.-854-??46+??deletion. Her NF2 history is positive for a right optic nerve sheath meningioma, CNIII schwannoma requiring radiation therapy and post gross total resection of right frontotemporal anaplastic meningioma followed by radiation. At age 15 she developed new onset weight loss and …

Machine Intelligence Framework For Predictive Modeling Of Co2 Concentration: A Path To Sustainable Environmental Management, Mohamed Abouhawwash, Mohammed Jameel, Sameh S. Askar

Sustainable Machine Intelligence Journal

Climate change poses critical challenges, necessitating accurate and timely monitoring of CO2 concentrations for sustainable environmental management. Traditional methods for CO2 prediction exhibit limitations in precision and scalability. This paper introduces a novel Machine Intelligence Framework (MIF) specifically designed for predictive modeling of CO2 concentration levels. Leveraging advanced machine learning algorithms and data processing techniques, MIF aims to address the existing research gap by offering enhanced accuracy and adaptability in CO2 forecasting. Motivated by the urgency to combat climate change, this research develops a comprehensive framework integrating predictive modeling with machine intelligence. The methodology involves algorithm design, data integration, and …

In Vivo Functional Characterization Of Egfr Variants Identifies Novel Drivers Of Glioblastoma, Kwanha Yu, Kathleen Kong, Brittney Lozzi, Estefania Luna-Figueroa, Alexis Cervantes, Rachel Curry, Carrie A Mohila, Ganesh Rao, Ali Jalali, Gordon B Mills, Kenneth L Scott, Benjamin Deneen

Faculty, Staff and Students Publications

BACKGROUND: Glioblastoma is the most common and aggressive primary brain tumor. Large-scale sequencing initiatives have cataloged its mutational landscape in hopes of elucidating mechanisms driving this deadly disease. However, a major bottleneck in harnessing this data for new therapies is deciphering "driver" and "passenger" events amongst the vast volume of information.

METHODS: We utilized an autochthonous, in vivo screening approach to identify driver, EGFR variants. RNA-Seq identified unique molecular signatures of mouse gliomas across these variants, which only differ by a single amino acid change. In particular, we identified alterations to lipid metabolism, which we further validated through an unbiased …

Looking For New, Trying Not To Lose The Old. Strategy Of The Main Library Of The Warsaw University Of Life Sciences, Wojcieh Woźniak

IATUL 2023 Slides

The Strategy of the Main Library of the Warsaw University of Life Sciences is a guide for developing the library in the next five years. This document covers the main activity of the library and shows the changes that library will undergo in the perspective of the years 2021-2025. The paper will discuss tasks and goals of the strategy and show the perspective of success according to the experiences of the last two years.

Genome Assembly Of The Brassicaceae Diploid Orychophragmus Violaceus Reveals Complex Whole-Genome Duplication And Evolution Of Dihydroxy Fatty Acid Metabolism, Fan Huang, Peng Chen, Xinyu Tang, Ting Zhong, Taihua Yang, Chinedu Charles Nwafor, Chao Yang, Xianhong Ge, Hong An, Zaiyun Li, Edgar B. Cahoon, Chunyu Zhang

Center for Plant Science Innovation: Faculty Publications

Orychophragmus violaceus is a Brassicaceae species widely cultivated in China, particularly as a winter cover crop in northern China because of its low-temperature tolerance and low water demand. Recently, O. violaceus has also been cultivated as a potential industrial oilseed crop because of its abundant 24- carbon dihydroxy fatty acids (diOH-FAs), which contribute to superior high-temperature lubricant properties. In this study, we performed de novo assembly of the O. violaceus genome. Whole-genome synteny analysis of the genomes of its relatives demonstrated that O. violaceus is a diploid that has undergone an extra whole-genome duplication (WGD) after the Brassicaceae-specific α-WGD event, …

Plant Breeding Advancements With “Crispr-Cas” Genome Editing Technologies Will Assist Future Food Security, M. Ahmad

Center for Plant Science Innovation: Faculty Publications

Genome editing techniques are being used to modify plant breeding, which might increase food production sustainably by 2050. A product made feasible by genome editing is becoming better known, because of looser regulation and widespread acceptance. The world’s population and food supply would never have increased proportionally under current farming practices. The development of plants and food production has been greatly impacted by global warming and climate change. Therefore, minimizing these effects is crucial for agricultural production that is sustainable. Crops are becoming more resilient to abiotic stress because of sophisticated agricultural practices and a better understanding of the abiotic …

No. 07: The Sdgs, Migrant Remittances And Food Security In Jamaica, Elizabeth Thomas-Hope

Migration and Food Security (MiFOOD) Network

This paper raises questions about the implications of migration for inclusive social development in Kingston, Jamaica. This is based on an assessment of the relationship between remittances and food security at the household level. Emphasis is placed on whether remittances have made a difference in reducing the food insecurity associated with poverty and thus could contribute to inclusive social development. A city-wide survey of Kingston based on a sample of households in communities spanning different socioeconomic sectors was conducted to provide the data. Three indicators were used to measure food security/insecurity. Since there were the highest levels of food insecurity …

The Investigation Of First-Year Wellness Programming In College To Reduce Stress And Anxiety Levels, Charlotte Muriel

Graduate Student Dissertations, Theses, Capstones, and Portfolios

The COVID-19 pandemic brought mental health to light, although college students have long been experiencing mental health struggles. First-year experience courses have been shown to relieve some of the stresses associated with the college transition (Holliday, 2014); however, the existing programming does not meet the needs of college students affected by the COVID-19 pandemic. Due to the increased need for mental health services and counseling on college campuses, there is a need for additional support for students dealing with wellness concerns.

This action research study used a three-phase exploratory sequential mixed methods design. Phase One included two audio-recorded focus group …

Reverse Phase Protein Array Profiling Identifies Recurrent Protein Expression Patterns Of Dna Damage-Related Proteins Across Acute And Chronic Leukemia: Samples From Adults And The Children's Oncology Group, Fieke W Hoff, Ti'ara L Griffen, Brandon D Brown, Terzah M Horton, Jan Burger, William Wierda, Stefan E Hubner, Yihua Qiu, Steven M Kornblau

Faculty, Staff and Student Publications

DNA damage response (DNADR) recognition and repair (DDR) pathways affect carcinogenesis and therapy responsiveness in cancers, including leukemia. We measured protein expression levels of 16 DNADR and DDR proteins using the Reverse Phase Protein Array methodology in acute myeloid (AML) (n = 1310), T-cell acute lymphoblastic leukemia (T-ALL) (n = 361) and chronic lymphocytic leukemia (CLL) (n = 795) cases. Clustering analysis identified five protein expression clusters; three were unique compared to normal CD34+ cells. Individual protein expression differed by disease for 14/16 proteins, with five highest in CLL and nine in T-ALL, and by age in …

Money Matters: Time For Prevention And Early Intervention To Address Family Economic Circumstances, Nick Axford, Vashti Berry

Peninsula Medical School

Child poverty is associated with poorer physical and mental health, negative educational outcomes and adverse long-term social and psychological consequences, all of which impact on service demand and expenditure. Until now, however, prevention and early intervention practice has tended to focus on enhancing inter-parental relationships and parenting skills (e.g., via relationship skills education, home visiting, parenting programs, family therapy) or child language, social-emotional and life skills (e.g., early childhood education, school-based programs, youth mentoring). Programs often target low-income neighborhoods or families but rarely address poverty directly. While there is substantial evidence for the effectiveness of such interventions in improving child …

Equipment As Art, Art As Equipment: Notes On Film, Architecture, And Martin Heidegger’S Philosophy Of Culture, Max Ryynänen, Petteri Kummala

Contemporary Aesthetics (Journal Archive)

Equipment (das Zeug) and the work of art are key concepts in Martin Heidegger’s philosophy of art. In The Origin of the Work of Art, Heidegger contrasts equipment, sometimes translated as ‘tool,’ with art. The term could, however, be useful for discussing art and culture. The “readiness-at-hand” of a hammer (part of the network of equipment) makes it transparent. We notice it only when it is broken. Heidegger posits this function as opposite to the way works of art push (Stoss) us out of our comfort zone to deal with our existential abyss. However, since they have a …

A Pilot Study Of The Association Between Maternal Mid-Pregnancy Cholesterol And Oxysterol Concentrations And Labor Duration., Todd C. Rideout, Jaclyn Wallace, Xiaozhong Wen, Vanessa M. Barnabei, Kai Ling Kong, Richard W. Browne

Manuscripts, Articles, Book Chapters and Other Papers

BACKGROUND: Previous animal model studies have highlighted a role for cholesterol and its oxidized derivatives (oxysterols) in uterine contractile activity, however, a lipotoxic state associated with hypercholesterolemia may contribute to labor dystocia. Therefore, we investigated if maternal mid-pregnancy cholesterol and oxysterol concentrations were associated with labor duration in a human pregnancy cohort.

METHODS: We conducted a secondary analysis of serum samples and birth outcome data from healthy pregnant women (N = 25) with mid-pregnancy fasting serum samples collected at 22-28 weeks of gestation. Serum was analyzed for total-C, HDL-C, and LDL-C by direct automated enzymatic assay and oxysterol profile including …

Assessment Of The Eligibility Of Triglyceride;Glucose Index And Triglyceride;High-Density Lipoprotein Cholesterol Ratio As Applicable Insulin Resistance Indices Among Overweight/Obese Egyptians, Ghada A. Omar, Mona A.E A. Hussein, Wafaa S. Hegab

Journal of Medicine in Scientific Research

Background Insulin resistance (IR) means the requirement of a higher insulin concentration to produce the expected biological effect. It was proposed that triglycerides–glucose index (TY G) and triglycerides–high-density lipoprotein cholesterol ratio (TG/HDL) were dependable, applicable, and less-expensive markers of IR. However, their results varied significantly among different ethnic groups. Aim To assess the eligibility of TY G and TG/HDL as IR indices among overweight and/or obese Egyptians. Patients and methods The participants in this cross-sectional study were 328 overweight and/or obese Egyptians. Their fasting blood glucose, TG, HDL, and fasting insulin blood concentrations were estimated. Homeostasis model assessment-insulin resistance (HOMA-IR), …

Syndemics And Social Factors: Infectious Disease Patterns Within The Population Of People Experiencing Homelessness In The United States, Kathleen Berzonsky

Honors Program: Senior Projects (Public)

Individuals experiencing homelessness are at increased risk of suffering from infectious diseases. This is due to a number of social factors and healthcare disparities, as well as the idea of syndemics, by which diseases cluster together to worsen disease burden. Current intervention strategies approach treatment from a post-infection perspective, but reducing transmission rates of infectious diseases within the population of people experiencing homelessness will require a shift in the healthcare framework. The issue of people experiencing homelessness must be viewed through a biosocial lens, focusing on preventative care and treatment. I provide an overview of the social factors governing infectious …

Could Vitamin D3 Be Effective In Prevention Of Risk Of Preeclampsia Among Pregnant Women?, Adel G. Allam, Ibrahim M. El-Maghraby

Journal of Medicine in Scientific Research

Background Preeclampsia, a pregnancy disease marked by high blood pressure and proteinuria, affects 2–8% of all pregnancies. Preeclampsia can be life-threatening for both the mother and the fetus and is still a significant cause of maternal and neonatal morbidity and mortality. The goal of this study was to assess if there was a link between maternal serum vitamin D3 levels and the severity of preeclampsia. Patients and methods A case–control study was conducted on 70 pregnant women at Shebin El Kom Teaching Hospital. All pregnant women were divided into two groups: group A included 30 apparently healthy pregnant women (control …

Social Media And Women Empowerment In Nigeria: A Study Of The #Breakthebias Campaign On Facebook, Deborah Osaro Omontese

USF Tampa Graduate Theses and Dissertations

This paper examines how the March 2022 #BreakTheBias campaign on Facebook was used as an empowerment platform in Nigeria, where women experience gender disparity. Research on the role of social media in women’s empowerment in Nigeria is an area that has not been fully studied. Previous studies have looked at women’s empowerment mainly through an educational or political lens, neglecting how social media have also been effective in empowering women. Other researchers have studied how women utilize social media platforms for leisure, entertainment, and media sharing. In the present study, non-probability sampling was used to identify 20 posts that convey …

The Nairobi Declaration-Reducing The Burden Of Dementia In Low- And Middle-Income Countries (Lmics): Declaration Of The 2022 Symposium On Dementia And Brain Aging In Lmics, Gladys E. Maestre, Maria Carrillo, Raj N. Kalaria, Daisy Acosta, Larry Adams, Thierry Adoukonou, Upal Roy, Noe Garza, Jennifer Cahn, Deepa Roy

School of Medicine Publications

Delegates of the 2022 Symposium on Dementia and Brain Aging in Low- and Middle-Income Countries, representing over 40 countries, met in Nairobi, Kenya, December 5–9 to highlight advances in dementia prevention, diagnosis, care, and research, as well as explore the future needs of the global community.

Muscular Strength Predicts Phase Angle In Breast Cancer Survivors, Trevor Short, Cheri Teranishi-Hashimoto, Paulette Yamada

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 4: Issue 1, Article 4, 2023. Phase angle (PhA) has emerged as a prognostic indicator of survival and quality of life (QOL) in cancer patients. Identifying measures of physical fitness that correlate with PhA can provide guidance toward optimizing cancer rehabilitation programs and future research. The purpose was to examine the relationship between PhA and physical fitness in breast cancer survivors. Sixty-three breast cancer survivors (60 ± 9 years, PhA 4.59±0.52, mean±SD) completed assessments for muscular strength, muscular endurance, cardiorespiratory endurance, flexibility, and body composition. PhA and body composition were measured using bioimpedance …

An Evaluation Of Grazing Value Of Maize And Companion Crops For Wintering Lactating Ewes, E. A. Van Zyl, C. S. Dannhauser

IGC Proceedings (1977-2023)

Northwestern KwaZulu-Natal (KZN), in South Africa, is well known for its sheep production from natural rangeland in summer (October to May). During winter, however, the nutritional value of the rangeland cannot maintain young growing sheep or pregnant and lactating ewes. With this in mind, Lyle (1991) suggested the use of planted pastures for the winter. Crichton et al. (1998) and Esterhuizen & Niemand (1989) suggested the use of maize crop residues for both cattle and sheep during winter, whereas Moore (1997) evaluated grazing (not harvested) maize for this purpose. He found that the crude protein content of the crop was …

Production Per Animal And Use Of Intake Estimatives To Predicted Animal Productivity In Pennisetum Purpureum Cv. Mott And Cynodon Spp Cv. Tifton 85 Pastures, Fernando L. F. De Quadros, A. R. Maixner, Gilberto V. Kozloski, D. P. Montardo, A. Noronha, D. G. Bandinelli, M. Da S. Brum, N. D. Aurélio

IGC Proceedings (1977-2023)

Dairy production is a very important activity in southern Brazil, being an essential source of income to small household farms. Milk production from pastures is an alternative to reduce costs in dairy systems. Some C4 grasses, such as dwarf elephant grass (DEG) and Tifton 85, have presented high animal production per animal and per area. Although studies evaluating milk production from these pastures are rare in south Brazil, in vitro studies have demonstrated that the nutritional value of these forages is higher than production registered in grazing. So, it is possible that, in spite of a high intrinsic nutritional value, …

Ingestive Behaviour Of Steers In Native Pastures In Southern Brazil, C. E. Pinto, Paulo C. De F. Carvalho, A. Frizzo, J. A.S.F. Júnior, T. M. S. Freitas, Carlos Nabinger

IGC Proceedings (1977-2023)

The Campos biome, particularly its native pastures, is the main resource for livestock production in southern Brazil (Boldrini, 1997). It has a huge floristic diversity in which more than 400 grass and 150 legume species are found. These pastures are very heterogeneous with horizontal as well as vertical structure. Hodgson (1985) stressed the importance of sward structure upon diet selection, emphasizing the difficulties animals could have to access all pasture layers in temperate pastures. This experiment investigates how variation in herbage allowance along the growing season influences the ingestive behaviour of steers.

Translational Genomics For Alfalfa Varietal Improvement, G. D. May

IGC Proceedings (1977-2023)

Key points

1. Medicago truncatula is a model legume with available mapping, genome, and RNA, protein and metabolite profiling databases and genetically diverse populations.
2. Genomics resources developed for M. truncatula have application in the study and improvement of alfalfa making it an excellent model for this forage legume.

Protection Of Agrobiodiversity: Model Calculations In Rhineland-Palatia: Costs And Implications For Farmers, H. Bergmann

IGC Proceedings (1977-2023)

Biological conservation and production use the same areas of land in less favoured areas. Grassland in these areas makes an important contribution to the protection of agro-biodiversity. However, under existing market conditions and production needs, the use of low yielding grasslands is not economically efficient. The objective of this study was to analyze the economic consequences of different mowing strategies in a small region in Rhineland-Palatia (Germany) that served the protection of two butterfly species.

Managing Resources By Grazing In Grasslands Dominated By Dominant Shrub Species, D. Magda, Cyril Agreil, Michel Meuret, E. Chambon-Dubreuil, P. -L. Osty

IGC Proceedings (1977-2023)

The European natural grasslands are attracting new attention because of their environmental value as habitats for threatened fauna and flora species and their contribution to the diversity of landscapes. Those responsible for the implementation of the European agri-environmental policy are hence encouraging livestock farmers to adopt grazing practices that contribute to the conservation of grassland biodiversity especially by limiting encroachment by dominant shrubs. However, current scientific knowledge and technical information are often insufficient to connect flock feeding and the impact of grazing on shrub dynamics and livestock farmers are not very enthusiastic about restoring or conserving “plant mosaics” including shrubs …