Digital Commons Network^™

Biochemical And Genetic Mechanisms Underpinning Escherichia Coli Cell Cycle And Growth Transitions, Josiah Morrison

Open Access Dissertations

Bacterial cell division and the decision to divide is a process that requires a wide variety of factors. In bacterial cells, such as the widely studied Escherichia coli, a large protein complex, termed the Z-ring, assembles at midcell to enable division. The Z-ring is subject to many regulators which govern the spatiotemporal positioning of the ring. The Z-ring is made up of the eukaryotic tubulin homolog FtsZ, which polymerizes to recruit division proteins to the midcell. FtsZ polymers are tethered to the cytoplasmic membrane by the actin homolog FtsA. Together, FtsZ and FtsA act as the first step in …

Covid-19 Return To Sport: Collegiate Baseball Injury Prevalence Analysis, Troy B. Puga, Joshua Schafer, Grace Thiel, Laura Ramaker, Tejas Patel, Kevin Treffer

Faculty and Staff Publications

In the spring of 2020, the COVID-19 pandemic rapidly brought college baseball to a halt. Most college baseball players saw disruption of their training and development due to interruptions of their seasons. With these interruptions, it is necessary to examine the effects of the COVID-19 pandemic on collegiate baseball injury epidemiology. Data from a small-school Midwest collegiate conference was tallied for the number of collegiate conference injuries per year, and for the injuries per anatomic region. An unpaired t-test was conducted upon the overall conference mean injuries per year and on each anatomic region for the mean injuries per region …

On Fires, Floods, And Federalism, Andrew Hammond

Articles by Maurer Faculty

In the United States, law condemns poor people to their fates in states. Where Americans live continues to dictate whether they can access cash, food, and medical assistance. What’s more, immigrants, territorial residents, and tribal members encounter deteriorated corners of the American welfare state. Nonetheless, despite repeated retrenchment efforts, this patchwork of programs has proven remarkably resilient. Yet, the ability of the United States to meet its people’s most basic needs now faces an unprecedented challenge: climate change. As extreme weather events like wildfires and hurricanes become more frequent and more intense, these climate-fueled disasters will displace and impoverish more …

Evaluation Of The Dynamic Indicators Of Basic Early Literacy Skills (Dibels 8th Edition) Measures For Use With The Energy Express Summer Literacy Program, Maria Boki

Graduate Theses, Dissertations, and Problem Reports (ETD)

The present study examines the suitability of the DIBELS 8^th edition assessment for use within the scope and context of the Energy Express summer literacy program. The 6-week summer program helps children in grades K-5 from rural and low-income West Virginia communities improve or maintain their reading levels over the summer months. Standard, grade-appropriate DIBELS 8^th subtests were administered to 1,065 participants at the beginning and end of Summer 2022. The resulting pre- and post-scores were cross-referenced with demographic data and attendance rates from 20 counties and 35 sites in West Virginia. They were then analyzed for correlations …

Survival Of Listeria Monocytogenes On Organic Honeycrisp And Fuji Apples During Storage At 5, 12 And 22.5◦C, Connor S. Freed

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study aimed to evaluate and model the survival of Listeria monocytogenes on organic HoneyCrisp (HC) and Fuji (FJ) apples during storage at various temperatures. Fresh organic HC and FJ apples (without wax coating) obtained from a local wholesale market were inoculated with a 2-strain mix of L. monocytogenes followed by storage at 5.0 (22.9% RH), 12.0 (37.0% RH), and 22.5oC (50.4% RH) for 60, 35, 7 days, respectively, and periodically (day-0 to 60) analyzing microbial populations. Surviving L. monocytogenes were spread-plated on Modified-Oxford agar after 10 or 100-fold serial dilution. Data were analyzed using the mixed-model procedure of SAS …

Clinical And Economic Burden Of Inflammatory Chronic Conditions Among Adults In The United States: Evidence From Three Nationally Representative Surveys, Mohammad Ikram

Graduate Theses, Dissertations, and Problem Reports (ETD)

A significant proportion of adults in the United States (US) suffer from various inflammatory chronic conditions (ICCs). They include a wide range of diseases such as diabetes, rheumatoid arthritis, asthma, and chronic obstructive pulmonary diseases. ICCs are not curable, and their management typically involves medications such as NSAIDs and corticosteroids. Recently, a whole health approach that places patients’ well-being at the center is increasingly being used for better management of ICCs. The whole health approach uses an integrated approach that includes multimodal management with complementary and alternative medicines (CAM) and prescription drugs. However, the use of CAM and prescription drugs …

Lipid Binding Properties Of Huntingtin As A Novel Therapeutic Target, Chathuranga Siriwardhana

Graduate Theses, Dissertations, and Problem Reports (ETD)

As protein aggregation is the defining hallmark of all amyloid diseases, a common therapeutic strategy is to develop molecules that inhibit aggregation. However, this approach has yielded limited success. Many amyloid proteins directly interact with lipid membranes. These interactions promote distinct aggregation pathways and often result in membrane damage leading to toxicity. As a result, directly targeting the ability of amyloids to bind lipid membranes represents a novel therapeutic strategy. As a proof of principle, the interaction between lipid membranes and mutant huntingtin protein (htt) aggregates was used to test this strategy. Mutant htt containing an expanded polygulatmine (polyQ) domain …

Effects Of Development On Hpa Function Following Pubertal Stress, Brittany D. Elliott

Graduate Theses, Dissertations, and Problem Reports (ETD)

For women, two of the greatest risk factors for affective disorders are adversity experienced during puberty and later becoming pregnant. We have created a translationally relevant mouse model where we address these complex risk factors. Previously, we discovered that pregnant mice (dams) that experienced chronic variable stress (CVS) during puberty display a blunted hypothalamic-pituitary-adrenal (HPA) response when exposed to an acute stressor. Interestingly, this alteration only first becomes apparent during pregnancy, which is a sensitive period for these effects due to normative neuroendocrine changes. Further investigation of the mechanisms underlying this dysfunction revealed altered gene expression in the paraventricular nucleus …

The Expectations, Experiences And Satisfaction Of Students Within A Nutrition And Dietetics Program Regarding Faculty Academic Advising Using A Prescriptive Or Developmental Advising Lens, Nettie Puglisi Freshour

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research study examined student’s expectation, experiences and satisfaction with faculty academic advising using a prescriptive vs. developmental lens. The intent was to explore the student’s expectations of academic advising, determine if their experiences aligned with their expectation and if this led to satisfaction of their academic advising. This study utilized the Systems theory to determine how academic advising is performed by the program and if that experience for the student is how the systems (institution, college, department, and program) are promoting the form of advising. This study utilized a qualitative case study approach, guided by three research questions. Data …

Ambient Ammonia Synthesis Via Microwave-Catalytic Materials And Plasma Chemistry, Siobhan Brown

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ammonia is critical to supporting human life on earth because of its use as fertilizer. The Haber-Bosch process to produce ammonia has been practiced for over 100 years. This process operates at high pressure and temperature to overcome the thermodynamic and kinetic limitations of the ammonia synthesis reaction thus researchers have tried to overcome it for decades. At present this process represents 1% of global energy usage and 2.5% of global CO₂ emissions. The proposed chemical looping ammonia synthesis approach seeks to reduce the environmental impact of this critical process and to elucidate microwave-catalytic principles.

This research aims to …

Exploring The Biological Basis Of Residual Feed Intake In Beef Cattle Using Multi-Omics Analysis., Godstime Ayobami Taiwo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Efficient feed utilization is critical for both economic sustainability and environmental responsibility in modern livestock production. While there has been extensive research, the multifaceted nature of feed efficiency remains complex, with many biological factors still unexplored. This dissertation examines the physiological foundations of feed efficiency by elucidating some of the complex biological mechanisms associated with residual feed intake (RFI) phenotype in beef cattle, using a range of Omics approaches. We hypothesized that metabolites related to amino acids, carbohydrates, and fatty acids could act as potential biomarkers for RFI. Through a chemical group-based metabolomics method, we identified enriched pathways in feed-efficient …

Descriptions Of Breast Self-Care From Inner-City African American And Hispanic Women Seeking Care In A Comprehensive Breast Care Center, Salamata Yoda

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: A plethora of inquiries has irrefutably revealed a decline in the death rates of breast cancer in some ethnic populations due to timely diagnoses, and enhancement in treatment measures. However, not all women have gained equal access to these innovations in healthcare. Certainly, as a repercussion of the noted disparity, the overall breast cancer prevalence among African American and Hispanic women has steadily escalated. Although no single test can detect all breast cancers early, practicing breast self-care consisting of breast self-awareness (BSA) in combination with other screening methods such as clinical breast exam (CBE), mammogram screening, and a suitable …

Nature Based Interventions For Human Health And Wellbeing, Samantha I. Moyers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background. Nature exposure is beneficial to human health and wellbeing, but the evidence base for nature-based interventions (NBI) is still developing. Heterogeneous naming conventions, study designs, and intervention contexts create difficulty in accessing and assessing the evidence for such programming. While NBI have been utilized in a wide array of human populations, no interventions have been reported among clinical health professions students. Aims. The objectives of this dissertation project were to review the existing body of NBI literature exploring health and wellbeing outcomes, and to conduct a feasibility trial of forest bathing, a type of NBI, among clinical students. Specifically, …

Investigating The Role Of Nudt7-Mediated Peroxisomal Coa Degradation In The Regulation Of Hepatic Coa Levels And Lipid Metabolism, Schuyler Dan Adams Vickers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coenzyme A (CoA) is a crucial cofactor required to support many essential metabolic processes, including fatty acid oxidation and synthesis, the TCA cycle, and bile acid synthesis. In order to support the proper functioning of these pathways, CoA levels within the cell must be properly regulated. The regulation of CoA levels is achieved through a balance between CoA synthesis and CoA degradation. While the regulation of CoA synthesis has been extensively characterized, the degradation of CoA has received less attention. As such, the contribution of CoA degradation to the regulation of metabolism is poorly understood. NUDT7 is a CoA-specific Nudix …

Addition Of Apple Pomace To Feeding Substrate On Growth, Development, And Survival Rates Of Yellow Mealworms (Tenebrio Molitor) And Characterization Of Their Proteins Extracted By A Ph-Shift Process, Michelle Duvall

Graduate Theses, Dissertations, and Problem Reports (ETD)

The weight changes, survival/mortality rates, and proximate compositions of Tenebrio molitor (yellow mealworm) larvae whose diets were supplemented with apple pomace were analyzed after a 4-week feeding study to examine the nutritional quality of the insects for use as a future potential food source. Going forward, the terms “mealworm(s)”, "yellow mealworm(s)”, “T. molitor” will be used interchangeably and will always refer to the same species. The mealworm larvae were randomly divided into two groups: in addition to standard worm chow, one group was given 15 g/week of non-nutritive water-storing polymer crystals and the other was given apple pomace …

The Creation Of An Adolescent Prediabetes Screening Protocol To Help With Early Prediabetes Identification, Brittany Parker

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

The Creation of an Adolescent Prediabetes Screening Protocol to Help with Early Prediabetes Identification

Brittany Parker, MSN, APRN, FNP-C

Problem: The American Diabetes Association (ADA) and the International Society for Pediatric and Adolescent Diabetes (ISPAD) recommend screening for prediabetes and type 2 diabetes mellitus (T2DM) in high-risk asymptomatic children and adolescents after puberty or at ten years of age, whichever occurs first. However, this recommendation has not been formally recognized by the state of West Virginia for integration into wellness child exam requirements, nor has it been made common practice in many primary care clinics to be flagged as …

Dietary Supplementation And Functional Foods: From Development To Clinical And Public Health Implications, Rachel Grace Newman

Graduate Theses, Dissertations, and Problem Reports (ETD)

A rigorous research process is the foundation for the development and ultimate recommendation for use of dietary supplements and functional foods. This dissertation uses three seemingly disparate studies to explore the diverse research methodologies used in dietary supplement and functional food development and use. The first study focuses on primary research on the development of microgreen lemon balm herbal tea, employing experimental methodologies to assess its nutritional and bioactive properties compared to adult lemon balm herbal tea. Results showed that adult lemon balm tea contained higher total phenolics, total flavonoids, rosmarinic acid, and antioxidant capacity than microgreen teas, with hot …

Forage Grass Growth Habit And Allelopathy In Birdsfoot Trefoil Germination And Establishment, Nathan El Blake

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birdsfoot trefoil (Lotus corniculatus L.) (BFT) is a high quality, tanniniferous forage legume that has been shown to mitigate gastrointestinal nematode (GIN) infection in sheep. Reliable establishment of birdsfoot trefoil in existing pastures is difficult due to BFT’s poor seedling vigor, susceptibility to crown and root rot, and, perhaps chiefly, competition from other pasture plants. Most existing pastures in the Northeastern United States are primarily composed of cool-season grasses, many of which can be highly competitive. A common form of interplant competition is allelopathy, which occurs when secondary metabolites produced by a plant inhibit a necessary growth process of …

Evaluating The Effects Of Modifying Mixing And Pelleting Parameters On Feed Quality, Pellet Production Rate, And Broiler Performance, Lucas E. Knarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, an experiment was conducted to evaluate the effect of different mixer types (MTY) and mix times (MTI) on mix uniformity (MU). A 2 (mix time) x 3 (mixer type) factorial was utilized in this experiment to create six treatments, each mixed in three randomized complete blocks. Mix times were designated adequate or inadequate, were based on the manufacturers’ recommendation, and varied based on the mixer utilized. The mixer types utilized included a vertical mixer, horizontal mixer, and transfer mixing system. The transfer mixing system can be described as a batch-to-mix system …

Analysis Of Experimental Antimicrobial Prevention Strategies Against Microbial Contamination Of Locally Harvested Poultry And Produce In West Virginia, Rebecca Breanne Stearns

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fresh produce and poultry have been associated with several foodborne illness outbreaks. Strategies to mitigate these risks are a much-needed research priority; therefore, five experiments were conducted to analyze pathogen cross-contamination capabilities on produce and poultry. Microbial contamination prevention and reduction strategies were also studied.

In Chapter Two, three antimicrobial delivery methods in combination with increasing concentrations of hydrogen peroxide-peroxyacetic acid (H₂O₂-PAA) (0.05-0.25 ml/dL) were compared to evaluate their ability to reduce and prevent cross-contamination of Listeria monocytogenes (L. monocytogenes) and Listeria innocua (L. innocua) on inoculated apples. Apples have been …

Evaluate A Mixer Of Hydrogen Peroxide And Peroxyacetic Acid To Mitigate Microbial Cross-Contamination Of Salmonella Typhimurium And The Surrogate Enterococcus Faecium During Triple-Washing Of Butternut Squash, Jesica M. Temple

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Recent foodborne illness outbreaks have been linked to fresh fruits and vegetables. For public safety, awareness of the risks of contamination from foodborne pathogens that can occur during post-harvest farming practices is essential to prevent foodborne outbreaks. Controlling the spread of foodborne pathogens on produce requires proper washing procedures, antimicrobials and doses. Given the speed and diversity of the produce being distributed, local incidents may become international emergencies. Methods: Studies were included if they assessed the efficacy of antimicrobials and various washing procedures and had to conclude the mitigation of cross-contamination of foodborne pathogens on produce grown in the …

Cluster Homolog Of Immunoglobulin-Like Receptor Genes In Chicken Immune Responses, Brandi A. Sparling

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the identity and role of immunoglobulin-like (Ig-like) receptors in chickens, with focus on their implications in disease and disease progression. These receptors, wisely expressed across immune cells, interact with the major histocompatibility complex (MHC) class I molecules to modulate immune responses in mammals. Due to the insufficient representation of chicken Ig-like receptors in online databases, this study systematically annotates the chicken Cluster Homolog of Immunoglobulin-like Receptors (CHIR) genes using advanced bioinformatic techniques, aligning with the release of the 7th edition of the chicken genome assembly that comprises builds for a broiler and layer chicken. The analysis identifies …

Exploring The Conceptualizations And Utilizations Of Learning Theories In Sport Settings, Kevin R. Lou

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to identify how integrating learning theories into the design of a formal university course helps facilitate students’ resources, goals, and orientations (Schoenfeld, 2011) of learning theories for their future career work in coaching, sport psychology consulting, or other sport-related professions. Sixteen students signed up for a fifteen-week fall semester course at a Mid-Atlantic university in America and were asked to annotate, create, and reflect upon examples of future work in their desired fields for their three major written assignments in the course. Students reflected on their learning experience through pre-and-post semi-structured interviews and most …

The Impact Of Single-Gendered Versus Coeducational Physical Education Settings On Female Adolescent Body Image And Self-Esteem: Resisting The Weight Of Body Image And Self-Esteem Issues In Adolescence, Jamison H. Stockslager

EWU Masters Thesis Collection

Resistance training is related to disease prevention, improved health, and positive psychosocial well-being in adults and adolescents. Further, resistance training is positively associated with body image and self-esteem. However, most adolescents do not meet activity guidelines, particularly for resistance training. Schools play an essential role for increasing physical activity. Little is known about the effects of different classroom formats for resistance training, i.e., single-gendered versus coeducational, on body image and self-esteem in female adolescents.

PURPOSE: To determine the effects of single-gendered versus coeducational resistance training on body image and self-esteem in adolescent females. METHODS: High school females (n = 30) …

Prevalence Of Colorectal Polyps And Associated Risk Factors In Egyptian Population: A Single Center Study, Hossam El Refaey, Hazem Hakim, Ahmed Saed

Mansoura Medical Journal

Background: Intestinal polyps are noticeable mucosal outgrowths that extend into lumen of gastrointestinal tract. In adults, the most frequently detected polyps are adenomatous polyps which are thought to be precursors of large bowel cancer. Colonoscopy is the most widely accepted method for colon cancer screening. Early detection and removal of adenomas can significantly reduce risk of colorectal cancer hence; it is of great importance to evaluate the prevalence of colorectal polyps in Egyptian population by diagnostic colonoscopy and associated risk factors. Objective: detect the prevalence of colorectal polyps in Egyptian population and associated risk factors. Patients and Methods: A cross …

Trxg And Pcg Regulation Of Cardiac Cell Division And Patterning In Embryonic Heart Development, Adam J. Farmer

All-Inclusive List of Electronic Theses and Dissertations

Congenital heart defects (CHD) are the most common developmental abnormalities seen at birth. Therefore, it is critical to understand the molecular mechanisms that ensure cardiogenesis proceeds correctly. These mechanisms include those facilitated by the essential evolutionarily conserved Trithorax Group (TrxG) and Polycomb Group (PcG) protein complexes that govern proper gene expression during development and cardiogenesis. The Drosophila melanogaster  dorsal vessel, a linear tube composed of both contractile cardial cells (CCs) from Seven-up (Svp) and Tinman (Tin) lineages and nephrocytic pericardial cells (PCs), has served as an excellent model system to uncover the roles of genes regulating proper cardiogenesis in mammals. …

The Effect Of A 5-Week Concurrent Training Program On Acft Scores, Ruck March Times, And Aerobic Capacity In Army Rotc Cadets, Cody Durbin

Dissertations and Theses @ UNI

Most Army ROTC programs conduct physical training (PT) only 3 days per week and no study has directly compared the effects of conducting PT 5 days per week on ACFT scores, ruck march performance, and aerobic capacity. Past research has indicated greater performance adaptations with a concurrent training program and some research has shown that current ROTC PT programs may not be optimal in physically preparing ROTC cadets. Purpose: To examine the effectiveness of a 5-day per week periodized tactical strength and conditioning program on ACFT scores, and aerobic capacity in Army ROTC cadets. Methods: Eight ROTC cadets from UNI …

Imaging Supermolecular Interactions Of The Pharmaceutical- Cocrystal Of Apigeninnicotinamide Binding With Serum Albumin, Yuting Sun, Ming Guo, Xiaoxue Zhao, Ronghui Wu

Turkish Journal of Chemistry

In this paper, a new pharmaceutical cocrystal was synthesized using apigenin (AP) and pharmaceutically acceptable conformer nicotinamide (Nico), and the drug delivery between AP-Nico pharmaceutical cocrystal and human serum albumin (HSA) in vivo was studied at atomic scale. The pharmaceutical cocrystal was characterized using Fourier-transform infrared (FTIR) spectroscopy, 1H NMR spectroscopy, differential scanning calorimetry (DSC), and powder X-ray diffraction (PXRD), and the self-assembling mechanism was explored. The dissolution and cumulative release in vitro were investigated. Molecular dynamic (MD) simulation combined with fluorescence spectroscopy was used to study the delivery mechanism of AP-Nico to HSA. The results showed that AP was …

Effect Of Okra Bast Fillers On Biodegradation Properties Of Poly(Vinyl Alcohol) Composites, Gazi Md Arifuzzaman Khan, Nazi̇re Deni̇z Yilmaz

Turkish Journal of Chemistry

This paper focuses on the effect of okra bast fillers on biodegradation of poly(vinyl alcohol) composites. Fibers were obtained from okra plant stems via biological degumming and subjected to different chemical treatments such as scouring, alkalizing, maleic anhydride treatment, and vinyl acetate grafting. The fibers were ground and PVA-okra bast filler composites were produced at 20 wt% fiber load via the solution casting method. The obtained composites were tested in terms of mechanical properties and exposed to biodegradation in soil. Effects of okra bast filler addition and chemical treatments on changes in mass, breaking strength, elasticity modulus, and breaking elongation …

Quantifying Farm-To-Fork Greenhouse Gas Emissions For Five Dietary Patterns Across Europe And North America: A Pooled Analysis From 2009 To 2020, Daniel Burke, Paul Hynds, Anushree Priyadarshini

Articles

Dietary patterns are inherently related to greenhouse (GHG) emissions via agricultural practices and food production systems. As the global population is predicted to increase from 8 billion (current) to 9.6 billion by 2050 added pressure will be placed on existing agricultural systems, resulting in increased GHG emissions thus exacerbating climate change. Therefore, there is an urgent need to understand present-day dietary patterns to shift to sustainable and healthy diets to mitigate GHG emissions and meet future climate targets. However, no review or pooled analyses of dietary pattern emissions from a farm-to-fork perspective has been undertaken to date. The current study …