Digital Commons Network^™

Assessment Of Antibodies In The Upper And Lower Human Respiratory Tract At Steady State And After Respiratory Viral Infection, Marios Koutsakos, Jackson S Turner, M Cristina Vazquez Guillamet, Daniel Reynolds, Tingting Lei, Derek E Byers, Ali H Ellebedy, Philip A Mudd

2020-Current year OA Pubs

OBJECTIVES: There is an increasing appreciation for the need to study mucosal antibody responses in humans. Our aim was to determine the utility of different types of samples from the human respiratory tract, specifically nasopharyngeal (NP) swabs obtained for diagnostic purposes and bronchoalveolar lavage (BAL) obtained in outpatient and inpatient settings.

METHODS: We analysed antibody levels in plasma and NP swabs from 67 individuals with acute influenza as well as plasma and BAL from individuals undergoing bronchoscopy, including five control subjects as well as seven moderately and seven severely ill subjects with a respiratory viral infection. Levels of α2-macroglobulin were …

Tourette Syndrome Research Highlights From 2022, Andreas Hartmann, Per Andrén, Cyril Atkinson-Clément, Virginie Czernecki, Cécile Delorme, Nanette Marinette Monique Debes, Kirsten Müller-Vahl, Peristera Paschou, Natalia Szejko, Apostolia Topaloudi, Keisuke Ueda, Kevin J. Black

2020-Current year OA Pubs

This is the ninth yearly article in the Tourette Syndrome Research Highlights series, summarizing selected research reports from 2022 relevant to Tourette syndrome. The authors briefly summarize reports they consider most important or interesting.

Severe Central Nervous System Demyelination In Sanfilippo Disease, Mahsa Taherzadeh, Erjun Zhang, Irene Londono, Benjamin De Leener, Sophie Wang, Jonathan D Cooper, Timothy E Kennedy, Carlos R Morales, Zesheng Chen, Gregory A Lodygensky, Alexey V Pshezhetsky

2020-Current year OA Pubs

INTRODUCTION: Chronic progressive neuroinflammation is a hallmark of neurological lysosomal storage diseases, including mucopolysaccharidosis III (MPS III or Sanfilippo disease). Since neuroinflammation is linked to white matter tract pathology, we analyzed axonal myelination and white matter density in the mouse model of MPS IIIC

METHODS: Brain and spinal cord tissues of human MPS III patients, 6-month-old

RESULTS: Analyses of CC and spinal cord tissues by immunohistochemistry revealed substantially reduced levels of myelin-associated proteins including Myelin Basic Protein, Myelin Associated Glycoprotein, and Myelin Oligodendrocyte Glycoprotein. Furthermore, ultrastructural analyses revealed disruption of myelin sheath organization and reduced myelin thickness in the brains …

Evaluation Of Gliovascular Functions Of Aqp4 Readthrough Isoforms, Shayna M Mueller, Kelli Mcfarland White, Stuart B Fass, Siyu Chen, Zhan Shi, Xia Ge, John A Engelbach, Seana H Gaines, Annie R Bice, Michael J Vasek, Joel R Garbow, Joseph P Culver, Zila Martinez-Lozada, Martine Cohen-Salmon, Joseph D Dougherty, Darshan Sapkota

2020-Current year OA Pubs

Aquaporin-4 (AQP4) is a water channel protein that links the astrocytic endfeet to the blood-brain barrier (BBB) and regulates water and potassium homeostasis in the brain, as well as the glymphatic clearance of waste products that would otherwise potentiate neurological diseases. Recently, translational readthrough was shown to generate a C-terminally extended variant of AQP4, known as AQP4x, which preferentially localizes around the BBB through interaction with the scaffolding protein α-syntrophin, and loss of AQP4x disrupts waste clearance from the brain. To investigate the function of AQP4x, we generated a novel AQP4 mouse line (AllX) to increase relative levels of the …

The Actin-Bundling Protein, Pls3, Is Part Of The Mechanoresponsive Machinery That Regulates Osteoblast Mineralization, Samantha M Chin, Carmela Unnold-Cofre, Teri Naismith, Silvia Jansen

2020-Current year OA Pubs

Plastin-3 (PLS3) is a calcium-sensitive actin-bundling protein that has recently been linked to the development of childhood-onset osteoporosis. Clinical data suggest that PLS3 mutations lead to a defect in osteoblast function, however the underlying mechanism remains elusive. To investigate the role of PLS3 in bone mineralization, we generated MC3T3-E1 preosteoblast cells that are stably depleted of PLS3. Analysis of osteogenic differentiation of control and PLS3 knockdown (PLS3 KD) cells showed that depletion of PLS3 does not alter the first stage of osteoblast mineralization in which a collagen matrix is deposited, but severely affects the subsequent mineralization of that matrix. During …

Evaluating The Efficacy Of Purchased Antisense Oligonucleotides To Reduce Mouse And Human Tau In Vivo, Pranav Vemula, Kathleen M Schoch, Timothy M Miller

2020-Current year OA Pubs

Many preclinical and clinical studies support the use of antisense oligonucleotides (ASOs) as effective therapeutic strategies. However, acquiring ASOs for research purposes may be limited by partnerships with the pharmaceutical companies. Our lab previously developed an effective ASO strategy to lower human tau and reverse pathology in aged tauopathy model mice. Testing the efficacy of purchased tau lowering ASOs would provide support for these reagents as broad research tools. Purchased mouse and human tau lowering ASOs were infused or injected intracerebroventricularly into wildtype and tau transgenic mice. Following treatment, brain tissue evaluated for ASO distribution and levels of tau mRNA, …

The Role Of Vaccination Route With An Adenovirus-Vectored Vaccine In Protection, Viral Control, And Transmission In The Sars-Cov-2/K18-Hace2 Mouse Infection Model, Alexandria Dickson, Elizabeth Geerling, E Taylor Stone, Mariah Hassert, Tara L Steffen, Taneesh Makkena, Madeleine Smither, Katherine E Schwetye, Jianfeng Zhang, Bertrand Georges, M Scot Roberts, John J Suschak, Amelia K Pinto, James D Brien

2020-Current year OA Pubs

INTRODUCTION: Vaccination is the most effective mechanism to prevent severe COVID-19. However, breakthrough infections and subsequent transmission of SARS-CoV-2 remain a significant problem. Intranasal vaccination has the potential to be more effective in preventing disease and limiting transmission between individuals as it induces potent responses at mucosal sites.

METHODS: Utilizing a replication-deficient adenovirus serotype 5-vectored vaccine expressing the SARS-CoV-2 RBD (AdCOVID) in homozygous and heterozygous transgenic K18-hACE2, we investigated the impact of the route of administration on vaccine immunogenicity, SARS-CoV-2 transmission, and survival.

RESULTS: Mice vaccinated with AdCOVID via the intramuscular or intranasal route and subsequently challenged with SARS-CoV-2 showed …

Recruitment Methods And Yield Rates For A Multisite Clinical Trial Exploring Risk Reduction For Alzheimer's Disease (Rrad), Amanda N Szabo-Reed, Monica Sewell, Ellen F Binder, Et Al.

2020-Current year OA Pubs

INTRODUCTION: The risk reduction for Alzheimer's disease (rrAD) trial was a multisite clinical trial to assess exercise and intensive vascular pharmacological treatment on cognitive function in community-dwelling older adults at increased risk for Alzheimer's disease.

METHODS: Eligibility, consent, and randomization rates across different referral sources were compared. Informal interviews conducted with each site's project team were conducted upon study completion.

RESULTS: Initially, 3290 individuals were screened, of whom 28% were eligible to consent, 805 consented to participate (87.2% of those eligible), and 513 (36.3% of those consented) were randomized. Emails sent from study site listservs/databases yielded the highest amount (20.9%) …

Leishmania Major- Derived Lipophosphoglycan Influences The Host's Early Immune Response By Inducing Platelet Activation And Dkk1 Production Via Tlr1/2, Olivia C Ihedioha, Anutr Sivakoses, Stephen M Beverley, Diane Mcmahon-Pratt, Alfred L M Bothwell

2020-Current year OA Pubs

BACKGROUND: Platelets are rapidly deployed to infection sites and respond to pathogenic molecules via pattern recognition receptors (TLR, NLRP). Dickkopf1 (DKK1) is a quintessential Wnt antagonist produced by a variety of cell types including platelets, endothelial cells, and is known to modulate pro-inflammatory responses in infectious diseases and cancer. Moreover, DKK1 is critical for forming leukocyte-platelet aggregates and induction of type 2 cell-mediated immune responses. Our previous publication showed activated platelets release DKK1 following

RESULTS: Here we probed the role of the key surface virulence glycoconjugate lipophosphoglycan (LPG), on DKK1 production using null mutants deficient in LPG synthesis (

CONCLUSION: …

Vancomycin-Induced Gut Microbial Dysbiosis Alters Enteric Neuron-Macrophage Interactions During A Critical Period Of Postnatal Development, Ellen Merrick Schill, Elisabeth L Joyce, Alexandria N Floyd, Sreeram Udayan, Brigida Rusconi, Shreya Gaddipati, Bibiana E Barrios, Vini John, Mitchell E Kaye, Devesha H Kulkarni, Jocelyn T Pauta, Keely G Mcdonald, Rodney D Newberry

2020-Current year OA Pubs

Vancomycin is a broad-spectrum antibiotic widely used in cases of suspected sepsis in premature neonates. While appropriate and potentially lifesaving in this setting, early-life antibiotic exposure alters the developing microbiome and is associated with an increased risk of deadly complications, including late-onset sepsis (LOS) and necrotizing enterocolitis (NEC). Recent studies show that neonatal vancomycin treatment disrupts postnatal enteric nervous system (ENS) development in mouse pups, which is in part dependent upon neuroimmune interactions. This suggests that early-life antibiotic exposure could disrupt these interactions in the neonatal gut. Notably, a subset of tissue-resident intestinal macrophages, muscularis macrophages, has been identified as …

The Purpose Of Cybersecurity Governance In The Digital Transformation Of Public Services And Protecting The Digital Environment, Maad Mijwil, Youssef Filali, Mohammad Aljanabi, Mariem Bounabi, Mariem Bounabi

Mesopotamian Journal of CyberSecurity

As it tries to incorporate computer-based technology into the public services provided by businesses or organisations, the digital transformation process is currently regarded one of the most prominent topics in circulation. Establishing fundamentals and points is necessary for digital transformation, as is relying on a specific set of employee talents and actively incorporating customers in the process's evolution. Cybersecurity has become an increasingly important issue for governments and businesses worldwide, and this shift towards digital services is a top priority for all of them. Accelerating the digital transformation process and the usage of its services are the vulnerabilities to cyberspace, …

Theoretical Background Of Cryptography, Rusul Mansoor Al-Amri, Dalal N. Hamood, Alaa Kadhim Farhan

Mesopotamian Journal of CyberSecurity

Cryptography is the practice of secure communication and the study of secure communication techniques. The theoretical foundations of cryptography include concepts such as cryptography, secure key exchange, digital signatures, and authentication. These technologies are used to protect the confidentiality, integrity, and reliability of information while it is being transmitted over a network or stored in digital form. The core of encryption is the concept of an encryption algorithm, which is a set of mathematical rules used to encrypt and decrypt data. The security of a cryptographic system depends on the strength of the underlying algorithm and the confidentiality of the …

Novel Convolutional Neural Networks Based Jaya Algorithm Approach For Accurate Deepfake Video Detection, Zahraa Faiz Hussain, Hind Raad Ibraheem

Mesopotamian Journal of CyberSecurity

Deepfake videos are becoming an increasing concern due to their potential to spread misinformation and cause harm. In this paper, we propose a novel approach for accurately detecting deepfake videos using the combination of Convolutional Neural Networks (CNNs) with the Jaya algorithm optimization. The approach is evaluated on two publicly available datasets, the DeepFake Detection Challenge (DFDC) dataset and the Celeb-DF dataset, and achieves state-of-the-art performance on both datasets. Our approach achieves an accuracy of 99.3% on the DFDC dataset and 97.6% on the Celeb-DF dataset, with high F1 scores indicating a high precision and recall for detecting deepfake videos. …

The Impact Of Blockchain Technique On Trustworthy Healthcare Sector, Nour Sadeq, Zeid Hamzeh, Ghalia Nassreddine, Tamima Elhassan, Tamima Elhassan

Mesopotamian Journal of CyberSecurity

Following the COVID epidemic, the healthcare sector faced numerous issues as telehealth became more prevalent and the necessity for a safe and efficient healthcare record system became critical. Many issues plague the healthcare industry today, including security, trust, data availability, and drug traceability. Blockchain technology is a relatively new technology that has demonstrated its effectiveness in a variety of industries, including finance, banking, bitcoin, and healthcare. This research investigates the impact of blockchain on the trustworthiness of healthcare. The authors adopt a descriptive-analytical methodology. The trustworthiness of the healthcare sector is considered a dependent variable. However, blockchain technology is considered …

Dobbs In A Technologized World: Implications For Us Data Privacy, Jheel Gosain, Jason D. Keune, Michael S. Sinha

All Faculty Scholarship

In June of 2022, the U.S. Supreme Court issued its opinion in Dobbs v. Jackson Women’s Health Organization, overturning 50 years of precedent by eliminating the federal constitutional right to abortion care established by the Court’s 1973 decision in Roe v. Wade. The Dobbs decision leaves the decision about abortion services in the hands of the states, which created an immediately variegated checkerboard of access to women’s healthcare across the country. This in turn laid bare a profusion of privacy issues that emanate from our technologized world. We review these privacy issues, including healthcare data, financial data, website tracking and …

The Psychology Of Science Denialism And Lessons For Public Health Authorities, Brenna Moreno, Molly J. Walker Wilson

All Faculty Scholarship

As it wreaked tragedy on the world, the outbreak of COVID-19 helped expose a pandemic of a different kind, one steeped in distrust and contrarianism. This movement, termed science denialism, has been lurking and undermining public health efforts for decades. Specifically, it is “the employment of rhetorical arguments to give the appearance of legitimate debate where there is none, an approach that has the ultimate goal of rejecting a proposition on which a scientific consensus exists.” Unlike skepticism, which is “doubt as to the truth of something” and works to progress both science and society, denialism is characterized by individuals’ …

Re-Regulating Dietary Supplements, Jessie L. Bekker, Alex Flores, Michael S. Sinha

All Faculty Scholarship

In 1994, Congress introduced the Dietary Supplement Health and Education Act (DSHEA) to create a regulatory framework for the dietary supplement industry. Since the passage of DSHEA nearly thirty years ago, U.S. adults have steadily increased their annual consumption of dietary supplements. The once $4 billion industry comprising approximately 4,000 products has swelled to a $40 billion trade with anywhere from 50,000 to 80,000 dietary supplements available over-the-counter.

Despite the increased market size of dietary supplements, the Food and Drug Administration’s (FDA) pre-market authority to regulate the introduction of dietary supplements into the stream of commerce has remained subdued. Under …

Protecting A Real Or Imagined Past: Justice Samuel Alito And The First Amendment, Derigan Silver, Dan V. Kozlowski

All Faculty Scholarship

This article examines the First Amendment jurisprudence of Justice Samuel Alito. In this article, we argue that the principles behind his decision-making are not always necessarily traditional methods of constitutional analysis, and litigants should understand the frames and lenses Alito uses to make decisions when making their arguments to him. The article concludes with a discussion of Alito’s overall approach to the law and some thoughts on how he is attempting to reshape the First Amendment. We write that, above all, it is clear he is seeking to protect a real or imagined past that, in his mind, is under …

Safety And Tolerability Results Of Atogepant For The Preventive Treatment Of Episodic Migraine From A 40-Week, Open-Label Multicenter Extension Of The Phase 3 Advance Trial, Brad C Klein, Rosa Miceli, Lawrence Severt, Peter Mcallister, Laszlo Mechtler, Jennifer Mcvige, Merle Diamond, Michael J. Marmura, Hua Guo, Michelle Finnegan, Joel M Trugman

Department of Neurology Faculty Papers

Background: Atogepant is a United States Food and Drug Administration-approved oral calcitonin gene-related peptide receptor antagonist for the preventive treatment of episodic migraine. The study objective was to evaluate the long-term safety and tolerability of atogepant in participants who completed the phase 3 ADVANCE trial (NCT03777059).

Methods: This 40-week, open-label extension trial (NCT03939312) monitored safety in participants receiving oral atogepant 60 mg once daily, followed by a four-week safety follow-up period.

Results: Of the 685 participants taking at least one dose of atogepant, the treatment period was completed by 74.6% of participants with a mean (standard …

What Factors Keep Late-Career Agriculture Teachers In Georgia Motivated To Continue In The Profession?, Catherine I. Davis

Murray State Theses and Dissertations

Most agriculture teachers leave the profession by the time they reach their mid-career phase. While there is significant research indicating factors why agriculture teachers choose to leave the career there is a void in the knowledge as to why late career agriculture teachers have persevered and are still choosing to stay in the classroom. This qualitative study identifies factors as to why agriculture teachers have chosen to stay in the profession. The researcher interviewed late career agriculture teachers in Georgia with 20 or more years in the classroom and are currently still teaching today. These interviews lead to common themes …

Effects Of Plasma Sample Handling On Equine Insulin Assays, Lauren Willett

Murray State Theses and Dissertations

Often, the assumption is made that the results of the various laboratory tests being run are accurate, and that abnormal results reflect a physiologic change occurring in the patient. Unfortunately, this assumption is not always true. Inaccurate results, for various reasons, are an inherent part of diagnostic testing. Having inaccurate results is often worse than having no results at all, because they can lead to an incorrect diagnosis or result in unnecessary testing, wasting the client’s financial resources, or potentially expose the patient to unnecessary risks in pursuit of the cause of an abnormality that does not exist. With appropriate …

The Effect Of An Afterschool Makerspace Program On Academic Achievement Of Sixth-Eighth Grade Students, Teresa Lampe

Murray State Theses and Dissertations

This quasi-experimental study investigated the effect of participation in an afterschool makerspace program on middle school students' academic achievement, grades, and school attendance. An experimental group of 237 sixth-eighth grade students participated in an afterschool makerspace program during the 2019-2020, 2020-2021, and 2021-2022 school years. A control group was selected using stratified random sampling of students from the same schools, grades, and special education designation who did not participate. The data included science and math NWEA scores, science and math grades, program attendance, school attendance, and gender. The data were analyzed using an independent t test with a 95% confidence …

An Exploratory Study Of Ergonomics In College Students, Wesley Ryan Tinker

Murray State Theses and Dissertations

Abstract

This study aimed to examine the relationship between poor ergonomic conditions and student physiological discrepancies. This examination provided great insight into students’ daily experiences and concerns for their futures. The methodology used for this study was quantitative as two source data sets were collected and analyzed. The tool REBA was used to measure the specific body angles while students sat working. A survey yielded great results, giving the students an opportunity to subjectively choose options.

This exploratory study allowed the researcher to use quantitative data to answer this study’s research questions. The research questions asked from this study were …

Self-Diagnosis & Pathologizing Normality During The Information Age, Chelsey Eaton

Murray State Theses and Dissertations

Self-diagnosis has become a concern for medical and mental health diagnoses. More people are engaging in self-diagnosis based on information that was obtained from the internet, including websites, social media platforms, and online forums. There are several reasons one may choose to self-diagnose. Some common reasons outlined in research are the desire to fit in with others who share similar characteristics and having a label to explain one’s experiences. Past research has shown that people frequently search for mental health information online, but they may not understand or may misinterpret the information that is found. This study aimed to address …

Halo Meets Horn: Influence Of Dissonant Information In Impressions, Amber Sanders

Murray State Theses and Dissertations

Abstract

The halo effect is a cognitive bias in impression formation that happens when people assume that when a person has a positive trait, such as physical attractiveness, the rest of their characteristics are also positive, leading to an overall positive perception of that person (Thorndike, 1920; Radeke & Stahelski, 2020). Conversely, the horns effect links together a negative trait, such as unattractiveness, with other seemingly negative traits (e.g., selfishness), leading to an overall negative perception (Radeke & Stahelski, 2020). However, these impression formation heuristics can lead to inaccurate impressions and poor judgments (Baumeister & Vohs, 2007). The current study …

An Analysis Of P-20 Education At San Ildefonso Pueblo In Northern New Mexico, United States, Martin Edward Strones

Murray State Theses and Dissertations

This research study provides an inquiry by conducting a qualitative analysis of the conditions and issues associated with students at the San Ildefonso Pueblo in Northern New Mexico. For decades the education of San Ildefonso and Native Americans who live in the State of New Mexico has been ignored. This has been just the way it was, that is, until Wilhelmina Yazzie, a Native American, saw her son struggling in an unsatisfactory school system and decided to take action. This case was originally filed in McKinley Country, New Mexico, in 2014, home to largely Native American students for the Navajo …

Pov - You Have Adhd: Examining Predictors Of Self-Diagnosis Of Adhd On Tiktok, Olivia Bowden

Murray State Theses and Dissertations

Online self-diagnosis refers to an individual using the internet and applying their own knowledge to diagnose themself with a disorder without the input of a professional (Farnood, 2021). Social media platforms, such as TikTok, have created an environment full of mental health related information that may not always be correct. Individuals on this app may believe the videos they watch and which may ultimately lead to self-diagnosis. There has been a trend of popular mental health disorders on appearing TikTok, such as ADHD. The present study sought to examine potential predictors of self-diagnosis of ADHD through TikTok. This study examined …

Examining The Moderating Effect Of Online Social Support On The Relationship Between Time Spent Gaming And Well-Being In Mmorpg Players, Amber Clayton

Murray State Theses and Dissertations

The video game industry has had a long-standing effect on players for decades. With the introduction of the Massive Multiplayer Online Role-Play Game (MMORPG) genre, gamers’ social world had changed drastically with the ability to communicate with players around the globe. Many social scientists have begun studying the effects on the MMORPG genre with social functioning and overall well-being in players (Colwell et al., 1995). The purpose of this thesis was to explore players who partook in MMORPGs and see if these online games affected their well-being and social support. The obtained results (N = 113) found that MMORPG gameplay …

The Impact Of Classroom Economies On Teacher Job Satisfaction, Amanda Hutchison

Murray State Theses and Dissertations

The significance of this study lies in its focus on high school teachers' job satisfaction after implementing a classroom economy strategy, with no previous research available on this specific relationship. The study addresses the critical issue of teacher turnover and its financial and educational implications. It also offers valuable insights for teacher preparation programs, colleges and universities, state departments of education, and classroom economy nonprofit companies.

Conserved Gene Signatures Shared Among Mapt Mutations Reveal Defects In Calcium Signaling, Miguel A Minaya, Sidhartha Mahali, Abhirami K Iyer, Abdallah M Eteleeb, Rita Martinez, Guangming Huang, John Budde, Sally Temple, Alissa L Nana, William W Seeley, Salvatore Spina, Lea T Grinberg, Oscar Harari, Celeste M Karch

2020-Current year OA Pubs

No abstract provided.