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A Novel Therapeutic Approach To Regulate Carex8 Protein Expression Through E6-Conjugated Cell Penetrating Peptides, Jared D. Compaleo

Browse all Theses and Dissertations

Adenovirus is a human pathogen that causes cold like symptoms in healthy individuals, which could be lethal in pediatrics and the immunocompromised. In polarized epithelia, adenovirus uses the eight-exon isoform of the Coxsackievirus and Adenovirus Receptor (CAREx8) on the apical surface, to facilitate infection. CAREx8 protein expression level is regulated by the scaffolding protein MAGI-1, using two of its PSD95/Dlg-1/ZO-1 (PDZ) domains: PDZ2 and PDZ4. We previously showed that CAREx8 is degraded after interaction with PDZ4 and rescued following interaction with PDZ2. We then developed a PDZ2 binding decoy molecule (E6) that blocks the interaction between CAREx8 and PDZ2, which …

The Role Of Schwann Cells In Nerve Injury: Forskolin-Mediated Camp Activation Upregulates Tnfα Expression Despite Nf-Κb Downregulation In Lps-Treated Schwann Cells, Caitlyn E. Henry, Angela L. Asirvatham Ph.D.

Student Research Poster Presentations 2023

Although Schwann cells are known to play a role in axonal regeneration following nerve injury and inflammation, the exact mechanism is unknown. This study explores two potential mechanisms: the NF-κB and cAMP pathways. The NF-κB pathway produces cytokines, such as TNFα, to regulate inflammation, whereas the cAMP pathway is anti-inflammatory and regulates Schwann cell proliferation via AKAP95 and cyclin D3. Although it is well-known that NF-κB and cAMP are involved in inflammation, not much is known regarding the effects of forskolin-mediated cAMP activation on LPS-mediated NF-κB activation in Schwann cells. In this study, RT4-D6P2T immortalized rat Schwann cells were treated …

Disregarding Uncertainty, Marginalizing Patients, Elissa Philip Gentry

Scholarly Publications

No abstract provided.

Evidence For Redefining Dietary Fiber, Joshua Daw

Undergraduate Research (Journal)

There are currently three macronutrients recognized within the field of dietetics due to their gram quantity intake and requirement for human health; this includes proteins, fats, and carbohydrates. Dietary Fiber, however, is not considered to be an independent macronutrient despite its gram quantity intake and requirement for human health. Additionally, Dietary Fiber is a net-zero energy yielding nutrient unlike other carbohydrates. Although Dietary Fiber should remain chemically categorized as a carbohydrate, for the purposes of dietetic prescription and education, Dietary Fiber should be redefined as the fourth macronutrient. This definition shift would illustrate both the importance and complexity of this …

Animal Shelter Management Of Feline Leukemia Virus And Feline Immunodeficiency Virus Infections In Cats, Julie Levy, Cynda Crawford, Keegan Spera, E. Susan Amirian, Paola Dezubiria

Diseases and Health

Approximately 5% of cats in animal shelters in the United States test positive for either feline leukemia virus (FeLV) or feline immunodeficiency virus (FIV), which translates to more than 100,000 positive cats managed by shelters each year. Little is known about the current status of retroviral management in animal shelters, particularly in regions burdened by chronic pet overpopulation and high shelter admissions, such as the southern United States. The purpose of this study was to describe feline retroviral management in Florida shelters. Shelters were surveyed on practices including selection of cats for testing, diagnostic techniques, and outcome options for cats …

Tuberculosis Myositis In Leg Musculature, Tho Nguyen, Md, Justin Jin, Md

Poster Presentations

No abstract provided.

Does An Educational Intervention Program On Infection Precautions Decrease Absentee Rates In Schools?, Shelby Florann Keltner, Madelyn Salonga

Nursing | Senior Theses

During our community health clinical rotation in a public school, we observed abundant absences in the kindergarten class, which led us to inquire: What is the possible cause for the multiple absences? Further, we noticed that the students had a hand hygiene knowledge deficit. These observations led us to hypothesize that due to direct contact transmission many absences were related to infection-related illnesses, and without proper technique and understanding of hand washing, infectious diseases will continue to have a high probability of transmission in schools. The objective of our investigation is to identify the prevalence of this issue in communities …

Local-Scale Impacts Of Water Hyacinth On Water Quality In A Hypereutrophic Lake, J. R. Corman, A. Z. Roegner, Z. Ogari, T. R. Miller, C. M. Aura

School of Natural Resources: Documents and Reviews

No abstract provided.

Gut Microbiome Composition Associated With Plasmodium Infection In The Eurasian Tree Sparrow, Sage D. Rohrer, Briana Q. Robertson, Lon M. Chubiz, Patricia G. Parker

Biology Department Faculty Works

Recent expansion of microbiome research has uncovered connections between resident microbial communities and blood parasite risk, establishing the potential for microbial disease treatments such as probiotics in the future. However, this field has largely focused on humans and model organisms, leaving much unknown about how microbial communities might directly or indirectly impact parasite infection in wild populations and non-mammals. To contribute to this knowledge base in wild birds, we collected fecal and blood samples from wild Eurasian tree sparrows (Passer montanus) in the United States to test for associations between blood parasite infection and the gut microbiome. We used a …

What Does This Word Say Mommy? Home Literacy Environments And Its Role On Parental Response To Miscues During Home Share Reading, Chiquta Inez Jenkins

Theses and Dissertations

The aim of this qualitative study was to examine home literacy environment (HLE) and how this environment affects the way parental responded to their children’s oral miscues during shared reading. In addition, parental behavior-specifically corrective feedback as it relates to children’s perceptions of reading in terms of their reading attitudes were examined as a part of this study. The participants in this study were five second grade families from Queens, NY attending a private elementary school during the Spring of 2021. The data of this study were collected through semi structured child and parent interviews, demographic information forms, the Home …

Lipid Alternations In The Plasma Of Covid-19 Patients With Various Clinical Presentations., Xiao Liang, Xin Qi, Jin Yang, Xiaorui Wang, Hongyu Qin, Fang Hu, Han Bai, Yixin Li, Chengsheng Zhang, Bingyin Shi

Faculty Research 2023

BACKGROUND: COVID-19 is a highly infectious respiratory disease that can manifest in various clinical presentations. Although many studies have reported the lipidomic signature of COVID-19, the molecular changes in asymptomatic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected individuals remain elusive.

METHODS: This study combined a comprehensive lipidomic analysis of 220 plasma samples from 166 subjects: 62 healthy controls, 16 asymptomatic infections, and 88 COVID-19 patients. We quantified 732 lipids separately in this cohort. We performed a difference analysis, validated with machine learning models, and also performed GO and KEGG pathway enrichment analysis using differential lipids from different control groups.

RESULTS: …

Prenatal Buprenorphine Exposure Reduces Expression Of Myelin Proteins In Neonatal Longs-Evans Rat, Christopher James Grahe

Theses, Dissertations and Capstones

In the USA and especially in WV, there has been a surge in the rise of opioid use disorder (OUD), and with it a rise in neonatal abstinence syndrome (NAS). What makes this rise in NAS so unfortunate is that the Medications for OUD (MOUD) do not prevent the development of NAS. Although the relationship between MOUD and NAS is still unclear, it is thought that buprenorphine, one of the main substances used to treat OUD in pregnant women, may feed into the development of NAS, which may affect mental and physiological development and cause other health problems. Via understanding …

A Case Of Embolic Stroke Due To Native Mitral Valve Pasteurella Multocida Infective Endocarditis, Alexia Foy-Crowder, Omar Kandah, Mohamed Faris

South Atlantic Division GME Research Day 2023

No abstract provided.

Cancer Immunotherapy: A Breakthrough In Patient Care, Abdou-Samad Kone, Nour El Imane Issam Salah, Amina Ghouzlani, Abdallah Badou

Health Sciences

Cancer is one of the world's leading causes of death. Conventional therapies, such as surgical resection, radiotherapy, and chemotherapy, have improved patient management and have shown overwhelming progress. However, the incidence and mortality rates among cancer patients are still extremely high, at approximately 20,5 % and 10,6 % for both sexes, respectively. This may be due to the heterogeneity of tumors, their special microenvironment, and the development of resistance to certain conventional therapies. The concept of immunotherapy provided a new perspective on patient treatment. The interaction of tumor cells with their microenvironment depends on different factors and conditions that promote …

Veterinary Medicine And The Covid-19 Pandemic: An International, Interdisciplinary Study Of A Globalwicked Problem, Daniella Fedak-Lengel

International ResearchScape Journal

Building on field research in Costa Rica and Belize, this study analyzes environmental and endangered animal protection policies, rights, and practices in Central America, and assesses impacts of veterinary science and conservation biology on animal welfare concerns. Informed by the recent surge in awareness regarding the spread of zoonotic diseases, given COVID-19, the study analyzes Manis javanica and the impact of illegal trafficking of this critically endangered animal. The project theorizes if awareness of zoonotic disease transmission, especially during a global pandemic, could be key to reducing sales, legal or illegal, of wild animals in order to mitigate zoonotic infection …

The Other Side Of The Acess Pyramid: A Healing, Sidney Shaw, M Rides At The Door

Walden Faculty and Staff Publications

No abstract provided.

Comparing The Performance Of A Targeted Pull-Down Assay To Shotgun Sequencing For Improving Respiratory Infectious Disease Surveillance, Monica R. Christian

Browse all Theses and Dissertations

Current surveillance focuses on well characterized pathogens such as influenza. Since 2000, there have been multiple outbreaks of respiratory disease. These outbreaks have demonstrated the need for robust and collaborative global efforts to identify, monitor, and contain novel respiratory viruses. This study aims to improve the ability of public health agencies to monitor and respond to respiratory disease outbreaks. Using five respiratory pathogens, this study compares a molecular capture technology from Twist Biosciences to the shotgun sequencing approach of whole transcriptome amplification (WTA, Qiagen) with the goal of determining which method is most effective using cost, usability, and sequencing quality …

A Walking Case Of Extensive Klebsiella Pneumoniae Mimicking Lung Cancer, Diem Phuc T. Nguyen, Rebecca L. Cooper, Maria Antonio-Miranda, Patricia Gilford

South Atlantic Division GME Research Day 2023

No abstract provided.

Cytomegalovirus And Nodular Gastric Lesions: A Case Report, Tyler Jones, Daniel Bishev, Vidya Kollu

North Florida Division GME Research Day 2023

No abstract provided.

Nontyphoidal Salmonella Causing Mycotic Aneurysms And Subsequent Vascular Graft Infection: A Case Report, Bernard Dankyi, Sandi Dunn, Zainab Saeed, Vincent Santi, Aneta Tarasuik Rusek

North Florida Division GME Research Day 2023

No abstract provided.

Institute Of Public Health Newsletter- Volume 7, Issue 3, Institute Of Public Health (Iph)

Institute of Public Health Newsletter (IPHNL) and Other Publications

No abstract provided.

Bacteria Commonly Associated With Central Nervous System Catheter Infections Elicit Distinct Csf Proteome Signatures, Matthew K. Beaver, Dragana Noe, Ishwor Thapa, Hesham Ali, Jessica Snowden, Tammy Kielian, Gwenn Skar

Journal Articles: Pathology and Microbiology

Background: Cerebrospinal fluid (CSF) shunt infection is a common and devastating complication of the treatment of hydrocephalus. Timely and accurate diagnosis is essential as these infections can lead to long-term neurologic consequences including seizures, decreased intelligence quotient (IQ) and impaired school performance in children. Currently the diagnosis of shunt infection relies on bacterial culture; however, culture is not always accurate since these infections are frequently caused by bacteria capable of forming biofilms, such as Staphylococcus epidermidis, Cutibacterium acnes, and Pseudomonas aeruginosa resulting in few planktonic bacteria detectable in the CSF. Therefore, there is a critical need to identify a …

Soil-Transmitted Helminthiases And Deworming Response In School-Aged Children In Retalhuleu, Guatemala, Rebeca Esther Vergara Greeno

Yale Medicine Thesis Digital Library

Background: Soil-transmitted helminthiases (STH) are amongst the most common diseases of poverty worldwide despite mass deworming campaigns. However, widespread use of benzimidazole anthelminthics in these campaigns has been associated with variable efficacy against three of the most common STHs, thus raising concerns regarding emerging resistance. Albendazole, in particular, has been shown to be least effective against Trichuris trichiura.

Methods: Our aim was to determine baseline prevalence and intensity of STH infections in rural school children living in Retalhuleu, Guatemala, and to evaluate the efficacy of single dose albendazole in infected children. We conducted a cross-sectional study with 557 children ages …

Trust And Subjective Knowledge Influence Perceived Risk Of Lead Exposure, Madeline Goebel, Chloe B. Wardropper

National Drought Mitigation Center: Faculty Publications

Lead exposure is a persistent environmental hazard that poses risks to human health. But motivating protective action is challenging with this low visibility hazard whose health effects are often subtle and chronic. Higher risk perception is generally associated with taking protective measures, so public health efforts prioritize risk messaging. Yet, little is known about perceptions of lead exposure risk among the U.S. public. Using cross-sectional data from a national survey of 1035 U.S. residents, we measured the role of trust in government management of lead and subjective knowledge about lead as predictors of perceived risk of lead exposure, controlling for …

Impact Of Medicaid Expansion On Substance Abuse Treatment, Kyle Chapman

Theses, Dissertations and Capstones

Introduction: The Medicaid expansion has insured millions of Americans since the passage of the Affordable Care Act in 2010. Among this newly insured population, many of the enrollees have been diagnosed with a substance abuse disorder. Patients who have a substance use disorder diagnosis typically have multiple conditions that are treated simultaneously. When these patients have to seek healthcare services they are often uninsured. Unfortunately, since the patients are uninsured, the most frequent healthcare access points have been through admissions to more expensive areas of healthcare delivery such as the emergency department and subsequent inpatient services. The goals of the …

"We Don't Know What We Want": The Tug Between Rights And Public Health Online, Jonathan Zittrain

Duquesne Law Review

Twitter and Facebook boast billions of subscribers, many of whom are real people. The companies are also roundly hated, particularly by tech experts-at least those who follow them for something other than their stock performance.¹ Objections to platforms' behavior are commonly expressed as amazement that they could be so obviously and consistently wrong in failing to police awful content their users post. There is also amazement about unobjectionable posts and comments from users that they take down.² That, in turn, has led to pressure for regulatory initiatives to push the companies into doing what they so clearly ought …

Hiv And Addiction Services For People Who Inject Drugs: Healthcare Provider Perceptions On Integrated Care In The U.S. South, Davis Bradford, Mariel Parman, Sara Levy, Wesli H. Turner, Li Li, Leah Leisch, Ellen Eaton, Kaylee B. Crockett

APC Fund

This qualitative study evaluates physician training and experience with treatment and prevention services for people who inject drugs (PWID) including medications for opioid use disorder (MOUD) and HIV pre-exposure prophylaxis (PrEP). The Behavioral Model of Healthcare Utilization for Vulnerable Populations was applied as a framework for data analysis and interpretation. Two focus groups were conducted, one with early career physicians (n = 6) and one with mid- to late career physicians (n = 3). Focus group transcripts were coded and analyzed using thematic analysis to identify factors affecting implementation of treatment and prevention services for PWID. Respondents identified that increasing …

Exploring Inmate Health In Prison: Health Issues, Interventions, And Suggestions To Improve Care, Jenna Healy

Undergraduate Review

No abstract provided.

Autonomy And Paternalism In Medicine, Claire Consadine

Soaring: A Journal of Undergraduate Research

No abstract provided.

Surviving The Apprehensive Methicillin-Sensitive Staphylococcus Aureus (Mssa) Bacteremia: A Case Of Bacteremia With An Undetermined Source Of Infection Leading To Suspected Cardioembolic Ischemia And Biochemical Septic Shock, Greisy D. Curbelo Sesin, Eduardo Marino-Urbino, Christopher Fontela, Melanio J. Rodriguez, Jorge A. Diaz, Shivaan C. Oomrigar, Ramon Mendoza Bello, Yoandy Rodriguez Fuentes

East Florida Division GME Research Day 2023

Introduction: This research presents a complex case of a 70-year-old male with acute metabolic encephalopathy and biochemical septic shock, later attributed to cardioembolic ischemia, resulting from Methicillin-Sensitive Staphylococcus Aureus (MSSA) bacteremia with an elusive source of infection.

Case Description: The 70-year-old patient, with a medical history of hypertension and aortic valve stenosis status post-Coronary Artery Bypass Grafting and bioprosthetic valve replacement on dual anti-platelet therapy (clopidogrel and aspirin), was admitted following an unwitnessed fall and a day of vomiting and generalized weakness. Upon arrival, the patient exhibited altered mental status, hypotension, and acidosis. Initial diagnostic workup raised suspicion of biochemical …