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Analyses Of Morphological Effects Of Cryopreservation On Plasmodium Yoelii Sporozoites, Lauren J. Topley

USF Tampa Graduate Theses and Dissertations

Malaria is a vector-borne disease and is a major global public health threat caused by the genus Plasmodium. Of the five species that infected humans, Plasmodium vivax and Plasmodium falciparum contribute the highest rates of morbidity and mortality. Research efforts to develop antimalarial drugs and effective vaccines is imperative to the prevention of future cases. Plasmodium has three reproductive stages: sporogonic, liver, and blood. Sporozoites are injected into the human host from an infected female Anopheles mosquito, which travel to the liver to initiate liver stage malaria. Since few sporozoites are successful, the liver stage is a bottleneck of the …

The Current And Future Burden Of Long Covid In The United States, Sarah M Bartsch, Kevin L Chin, Ulrich Strych, Danielle C John, Tej D Shah, Maria Elena Bottazzi, Kelly J O'Shea, Mckaylee Robertson, Colleen Weatherwax, Jessie Heneghan, Marie F Martinez, Allan Ciciriello, Sarah Kulkarni, Kavya Velmurugan, Alexis Dibbs, Sheryl A Scannell, Yanhan Shen, Denis Nash, Peter J Hotez, Bruce Y Lee

Faculty, Staff and Students Publications

Background: Long coronavirus disease (COVID), which affects an estimated 44.69-48.04 million people in the United States, is an ongoing public health concern that will persist as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to spread.

Methods: We developed a computational simulation model representing the clinical course, health effects, and associated costs of a person with long COVID.

Results: Simulations show that the average total cost of a long COVID case can range from $5084-$11 646 (assuming symptoms only last 1 year) with 92.5%-95.2% of these costs being productivity losses. Therefore, the current number of long COVID cases could cost …

Establishment Of A Reverse Genetics System For Studying Human Immune Functions In Mice, Priya Pal, Shuai Gao, Hongbo Gao, Xiwen Qin, Marina Cella, Qiankun Wang, Liang Shan

2020-Current year OA Pubs

Reverse genetics approaches in mice are widely used to understand gene functions and their aberrations in diseases. However, limitations exist in translating findings from animal models to human physiology. Humanized mice provide a powerful bridge to understanding human physiology and mechanisms of disease pathogenesis while maintaining the feasibility of working with small animals. Methods for generating humanized mouse models that allow scientists to probe contributions of particular genes have been rudimentary. Here, we established an efficient method for generating genetically modified human cord blood-derived CD34

Prognostic Value Of Lipoprotein(A) For Cardiovascular Events After Lower Limb Revascularization In Diabetic Patients With Chronic Limb-Threatening Ischemia, Federico Biscetti, Maria Margherita Rando, Maria Anna Nicolazzi, Flavia Angelini, Roberto Iezzi, Luis H. Eraso, Paul J. Dimuzio, Dario Pitocco, Massimo Massetti, Antonio Gasbarrini, Andrea Flex

Department of Surgery Faculty Papers

BACKGROUND: Chronic limb-threatening ischemia (CLTI) presents a major clinical challenge in patients with Type 2 Diabetes Mellitus (T2DM), requiring lower extremity revascularization (LER) to mitigate adverse cardiovascular and limb outcomes. Lipoprotein(a) (Lp(a)) has been implicated in cardiovascular risk, but its role in patients with T2DM and CLTI undergoing revascularization remains unclear. Thus, this study aimed to investigate the prognostic value of Lp(a) levels in diabetic CLTI patients for major adverse cardiovascular events (MACE), major adverse limb events (MALE), or both after LER.

METHODS: In this prospective cohort study of 158 individuals with T2DM and CLTI undergoing LER, baseline clinical data …

Comparative Transcriptomic Analysis Of Mouse Macrophages Infected With Live Attenuated Vaccine Strains Of Mycobacterium Tuberculosis, Raja Veerapandian, Barbara Yang, Areanna Carmona, Melina J. Sedano, Victoria Reid, Rodrigo Jimenez, Jessica Chacon, Chinnaswamy Jagannath, Enrique I. Ramos, Shrikanth Gadad, Subramanian Dhandayuthapani

School of Medicine Publications

The BCG vaccine has been used against tuberculosis (TB) for over a hundred years; however, it does not protect adults from pulmonary TB. To develop alternative vaccines against TB, we generated Mycobacterium tuberculosis H37Rv (Mtb)-derived vaccine strains by rationally deleting key virulent genes, resulting in single (SKO; ΔfbpA), double (DKO; ΔfbpA-ΔsapM), triple (TKO-D; ΔfbpA-ΔsapM-ΔdosR and TKO-Z; ΔfbpA-ΔsapM-Δzmp1), and quadruple (QKO; ΔfbpA-ΔsapM-Δzmp1-dosR) strains. To understand how macrophages, the host cells that defend against infection and process antigens for presentation to immune cells, respond to these vaccine strains, we performed transcriptomic analyses of mouse bone marrow-derived macrophages …

Global Diversity Of Soil-Transmitted Helminths Reveals Population-Biased Genetic Variation That Impacts Diagnostic Targets, Marina Papaiakovou, Andrea Waeschenbach, Olumide Ajibola, Sitara Sr Ajjampur, Roy M Anderson, Robin Bailey, Jade Benjamin-Chung, Maria Cambra-Pellejà, Nicolas R Caro, David Chaima, Rubén O Cimino, Piet Cools, Anélsio Cossa, Julia Dunn, Sean Galagan, Javier Gandasegui, Berta Grau-Pujol, Emma L Houlder, Moudachirou Ibikounlé, Timothy P Jenkins, Khumbo Kalua, Eyrun F Kjetland, Alejandro J Krolewiecki, Bruno Levecke, Adrian Jf Luty, Andrew S Macdonald, Inácio Mandomando, Malathi Manuel, Maria Martínez-Valladares, Rojelio Mejia, Zeleke Mekonnen, Augusto Messa, Harriet Mpairwe, Osvaldo Muchisse, Jose Muñoz, Pauline Mwinzi, Valdemiro Novela, Maurice R Odiere, Charfudin Sacoor, Judd L Walson, Steven A Williams, Stefan Witek-Mcmanus, D Timothy J Littlewood, Cinzia Cantacessi, Stephen R Doyle

Faculty, Staff and Students Publications

Soil-transmitted helminths (STHs) are intestinal parasites that affect over a billion people worldwide. STH control relies on microscopy-based diagnostics to monitor parasite prevalence and enable post-treatment surveillance; however, molecular diagnostics are rapidly being developed due to increased sensitivity, particularly in low-STH-prevalence settings. The genetic diversity of helminths and its potential impact on molecular diagnostics remain unclear. Using low-coverage genome sequencing, we assess the genetics of STHs within worm, faecal, and purified egg samples from 27 countries, identifying differences in the genetic connectivity and diversity of STH-positive samples across regions and cryptic diversity between closely related human- and pig-infective species. We …

One Health Policy Communities’ Perspectives On Zoonoses Risk Factors And Management: A Multiple Qualitative Methods Approach, Lisset M. Dumet, Erin Kenzie, Julia M. Goodman, Veronika Merino Osorio, Vanessa Cruz Chavez, Ruth Atto, Percy Vilchez Barreto, Seth O’Neal

OHSU-PSU School of Public Health Faculty Publications and Presentations

Zoonoses emergence and endemicity are serious global public health problems that disproportionately affect low-resource settings. One Health (OH) serves as a framework to understand the interconnection of zoonoses risk factors and incentivizes collaboration, coordination, and communication among sectors. However, OH encounters barriers related to power imbalances, gaps in coordination, and conflicts of interest among diverse communities of practice, which are groups that share a problem and domain of interest and engage in collective learning. This research aims to elucidate the mental models of policy communities, which are representations of the environment, and how humans explain and predict their surroundings to …

مُستوى مَعرِفة معلِّمي الدراساتِ الاجتماعيّة للتعليم ما بعد الأساسي في سَلطنة عُمان بالأوبئة. The Level Of Knowledge Of Social Studies Teachers In Post-Basic Education Schools In The Sultanate Of Oman About Epidemics, رحمة علي البدوي, سيف ناصر المعمري

Association of Arab Universities Journal for Education and Psychology

هدفتِ الدراسةُ الحالية تعرف مستوى معرفة معلمي الدراسات الاجتماعية للتعليم ما بعد الأساسي في سلطنة عُمان بالأوبئة، علاوة على كشفِ أثرِ مُتغيِّر النوعِ، والتخصص، وسنوات الخبرة، والمحافظة، ومعدل متابعة أخبار كوفيد19، ومصادر الحصول على المعلومات الصحية خلال الجائحة، ومدى الالتزام بالإجراءات الوقائية، وظَّفت الدراسة المَنهجَ الوصفي من خلال إعدادِ اختبار معارف تكوَّن من ستة محاور، هي: مفهوم الأوبئة، تاريخ الأوبئة، أسباب انتشار الأوبئة، الآثار المترتبة عن انتشار الأوبئة، طرق علاج الأوبئة، دور سلطنة عُمان في مكافحة الأوبئة. طُبقت على عينة مكونة من (200) من مُعلِّمي الدراسات الاجتماعيَّة في سلطنة عُمان. وكشفت النتائج أن مستوى معرفة المُعلِّمين بالأوبئة منخفضًا، كما بيّنت …

Measles Assessment And Treatment, Bob Underwood, Edward Lyon

Pediatrics in Practice: A CME Podcast

Eddie Lyon, MD, discusses concerns related to an uptick in measles cases, and how community providers can assess, limit exposure and guide patients to remain healthy.

Click here to stream the podcast episode.

Qualitative Insights Into Drug Use Safety Strategies And Social Support Among Sexual Minority Women Who Inject Drugs In Baltimore, Maryland, Jennifer L. Glick, Leanne Zhang, Aimee Huang, Megan Nguyen, Danielle German

School of Public Health Faculty Publications

Background: Sexual minority women (SMW) experience higher rates of substance use relative to heterosexual women in the U.S. but remain an under-studied population. SMW who inject drugs (SMWWID) navigate an unpredictable drug market and may experience complex relationships within their social spheres. We explore cisgender SMWWID’s strategies to maintain safety while injecting drugs, and the influence of their social support systems on those strategies. Methods: We conducted N = 16 phone-based, semi-structured, in-depth interviews with SMWWID in Baltimore, Maryland between June-October 2021. The interviews explored participants’ sexual orientation and gender identities, social networks and support systems, drug use behaviors and …

A Single High-Zinc Activation Enhancer Can Control Two Genes Oriented Head-To-Head In Caenorhabditis Elegans, Hanwenheng Liu, Brian Earley, Adelita Mendoza, Patrick Hunt, Sean Teng, Daniel Luke Schneider, Kerry Kornfeld

2020-Current year OA Pubs

Enhancers play critical roles in gene expression, but a full understanding of their complex functions has yet to be defined. The cellular response to excess zinc levels in Caenorhabditis elegans requires the HIZR-1 transcription factor, which binds the high-zinc activation (HZA) enhancer in the promoters of multiple target genes. Cadmium hijacks the excess zinc response by binding and activating HIZR-1. By analyzing the genome-wide transcriptional response to excess zinc and cadmium, we identified two positions in the genome where head-to-head oriented genes are both induced by metals. In both examples, a single predicted HZA enhancer is positioned between the two …

Beliefs About The Causes And Treatment Of Common Mental Illnesses And Suicidality In Rural Uganda, Yang Jae Lee, Rita Mbabazi, Ondine Jevremov, Shakira Nakaweesi, Ella Brodey, Jason Wykoff, Roshan Sivakumar, Rauben Kazungu, Ibrahim Ssekalo, Robert Rosenheck, Alexander C Tsai

2020-Current year OA Pubs

BACKGROUND: Mental illnesses constitute a large and escalating portion of the global burden of disease, particularly in low- and middle-income countries like Uganda. Understanding community perceptions towards mental illness is crucial for developing effective interventions.

AIMS: To explore beliefs about the perceived causes and treatment of common mental illnesses (depression, anxiety, alcohol use disorder) and suicidality in rural eastern Uganda.

METHOD: Qualitative study using 31 in-depth interviews and 4 focus group discussions with healthcare workers, community health workers, community leaders and general community members in Buyende District, Uganda. Vignettes were used to depict mental illnesses to elicit perceptions, and data …

Cord Blood Proteomics Identifies Biomarkers Of Early-Onset Neonatal Sepsis, Leena B. Mithal, Mark E. Becker, Ted Ling-Hu, Young Ah Goo, Sebastian Otero, Aspen Kremer, Surya Pandey, Nicola Lancki, Yawei Li, Yuan Luo, William Grobman, Denise Scholtens, Karen K. Mestan, Patrick C. Seed, Judd F. Hultquist

2020-Current year OA Pubs

BACKGROUNDSymptoms of early-onset neonatal sepsis (EOS) in preterm infants are nonspecific and overlap with normal postnatal physiological adaptations and noninfectious pathologies. This clinical uncertainty and the lack of reliable EOS diagnostics results in liberal use of antibiotics in the first days to weeks of life, leading to increased risk of antibiotic-related morbidities in infants who do not have an invasive infection. METHODSTo identify potential biomarkers for EOS in newborn infants, we used unlabeled tandem mass spectrometry proteomics to identify differentially abundant proteins in the umbilical cord blood of infants with and without culture-confirmed EOS. Proteins were then confirmed using immunoassay, …

High-Affinity Cd16a Polymorphism Associated With Reduced Risk Ofsevere Covid-19, Anita E Qualls, Tasha Tsao, Irene Lui, Shion A Lim, Yapeng Su, Ernie Chen, Dylan Duchen, Holden T Maecker, Seunghee Kim-Schulze, Ruth R Montgomery, Florian Krammer, Charles R Langelier, Ofer Levy, Lindsey R Baden, Esther Melamed, Lauren Ir Ehrlich, Grace A Mccomsey, Rafick P Sekaly, Charles B Cairns, Elias K Haddad, Albert C Shaw, David A Hafler, David B Corry, Farrah Kheradmand, Mark A Atkinson, Scott C Brakenridge, Nelson I Agudelo Higuita, Jordan P Metcalf, Catherine L Hough, William B Messer, Bali Pulendran, Kari C Nadeau, Mark M Davis, Ana Fernandez-Sesma, Viviana Simon, Monica Kraft, Christian Bime, Carolyn S Calfee, David J Erle, Joanna Schaenmann, Al Ozonoff, Bjoern Peters, Steven H Kleinstein, Alison D Augustine, Joann Diray-Arce, Patrice M Becker, Nadine Rouphael, Impacc Network, Jason D Goldman, Daniel R Calabrese, James R Heath, James A Wells, Elaine F Reed, Lewis L Lanier, Harry Pickering, Oscar A Aguilar

Faculty, Staff and Students Publications

CD16A is an activating Fc receptor on NK cells that mediates antibody-dependent cellular cytotoxicity (ADCC), a key mechanism in antiviral immunity. However, the role of NK cell-mediated ADCC in SARS-CoV-2 infection remains unclear, particularly whether it limits viral spread and disease severity or contributes to the immunopathogenesis of COVID-19. We hypothesized that the high-affinity CD16AV176 polymorphism influences these outcomes. Using an in vitro reporter system, we demonstrated that CD16AV176 is a more potent and sensitive activator than the common CD16AF176 allele. To assess its clinical relevance, we analyzed 1,027 patients hospitalized with COVID-19 from the Immunophenotyping Assessment in a COVID-19 …

Development And Validation Of A Novel Clinical Risk Score To Predict Hypoxaemia In Children With Pneumonia Using The Who Prepare Dataset, Rainer Tan, Arjun Chandna, Tim Colbourn, Shubhada Hooli, Carina King, Norman Lufesi, Eric D Mccollum, Charles Mwansambo, Joseph L Mathew, Clare L Cutland, Shabir A Madhi, Marta Nunes, Sudha Basnet, Tor A Strand, Kerry-Ann F O'Grady, Brad Gessner, Emmanuel Addo-Yobo, Noel Chisaka, Patricia Hibberd, Prakash M Jeena, Juan M Lozano, William B Maleod, Archana Patel, Donald M Thea, Ngoc Tuong Vy Nguyen, Marilla Lucero, Syed Mohammad Akram Uz Zaman, Shinjini Bhatnagar, Nitya Wadhwa, Rakesh Lodha, Satinder Aneja, Mathuram Santosham, Shally Awasthi, Ashish Bavdekar, Monidarin Chou, Pagbajabyn Nymadawa, Jean William Pape, Glaucia Paranhos-Baccala, Valentina S Picot, Mala Rakoto-Andrianarivelo, Vanessa Rouzier, Graciela Russomando, Mariam Sylla, Philippe Vanhems, Jianwei Wang, Romina Libster, Alexey W Clara, Fenella Beynon, Gillian Levine, Chris A Rees, Mark I Neuman, Shamim Qazi, Yasir Bin Nisar, World Health Organization Prepare Study Group

Faculty, Staff and Students Publications

Background: Hypoxaemia predicts mortality at all levels of care, and appropriate management can reduce preventable deaths. However, pulse oximetry and oxygen therapy remain inaccessible in many primary care health facilities. We aimed to develop and validate a simple risk score comprising commonly evaluated clinical features to predict hypoxaemia in 2-59-month-old children with pneumonia.

Methods: Data from seven studies conducted in five countries from the Pneumonia Research Partnership to Assess WHO Recommendations (PREPARE) dataset were included. Readily available clinical features and demographic variables were used to develop a multivariable logistic regression model to predict hypoxemia (oxygen saturation < 90%) at presentation to care. The adjusted log coefficients were transformed to derive the PREPARE hypoxemia risk score and its diagnostic value was assessed in a held-out, temporal validation dataset. The model and risk score were analysed by evaluating the area under the receiver operating characteristic curve (AUC), sensitivity and specificity.

Results: We included 14 …

Monitoring Of African Horse Sickness Virus-Specific Antibodies In Horses, Northeastern Thailand, Napawan Bunpapong, Kitikhun Udom, Ekkapat Chamsai, Patchara Phuektes, Suphattra Jittimanee, Suphannika Phutthachalee, Supassama Chaiyawong, Kamonpan Charoenkul, Alongkorn Amonsin Prof. Dr.

The Thai Journal of Veterinary Medicine

African horse sickness (AHS) is caused by infection with the African horse sickness virus (AHSV). In March 2020, AHS was first reported in Thailand due to the importation of subclinically infected host animals, such as zebras. In this study, we conducted a serological survey for AHSV-specific antibodies across 21 horse farms in the northeastern provinces (n=3) of Thailand. A total of 600 serum samples were collected for cross-sectional (n = 292) and longitudinal serological monitoring (n = 308). A cross-sectional study was conducted in 2020, during which serum samples (n = 292) were collected and tested for AHS antibodies using …

Louis Pasteur: The Man Who Said, "Science Brings Men Closer To God", Alan L. Gillen, Adam Russell

Faculty Publications and Presentations

July 6, 2025, marks the 140-year anniversary of the first successful treatment of the rabies virus, discovered by Louis Pasteur. Louis Pasteur’s contributions and reputation are ever prevalent today, with “pasteurized” products being the signature of his legacy in microbiology. Through the creation of the “pasteurization” process (the gentle heating of food and drink products to eliminate pathogens), Louis Pasteur eloquently stated his motive for scientific discovery: “Man’s first look at the universe discovers only variety, diversity, multiplicity of phenomena. Let this gaze be enlightened by science . . . and simplicity and unity shine everywhere.” Ultimately, Pasteur’s work sought …

Exploring Sex-Specific Clinical Features In Chinese Patients With Takotsubo Syndrome, Hong-Yi Duan, Ling-Lin Wang, Ling-Chun Lyu, Zhen-Hua Shi, Xiao-Ping Lin, Zheng-Yang Yu, Guo-Hua Fan, Jing Chen, Xin-Hong Yang, Wei Deng, Chang-Wu Xu, Yan Huang, Qing Zhou, Jin-Ling Chen, Hong-Ning Song, Dong Hu, Jin-Qiu Liu, Hector Barajas-Martinez, Charles Antzelevitch, Kan Liu, Hong Jiang, Dan Hu

Department of Medicine Faculty Papers

BACKGROUND: Takotsubo syndrome (TTS) is considered as a transient acute heart failure syndrome predominant in females. However, data on Chinese TTS patients are limited. Therefore, this study aimed to investigate the clinical characteristics of Chinese TTS patients, identify prognostic markers.

METHODS: A total of 258 TTS patients were enrolled. Data on clinical characteristics and in-hospital major adverse cardiovascular events (MACE) were collected and analyzed. The latter included severe heart failure, shock, malignant arrhythmia, and death.

RESULTS: The average age of TTS patients was 59.3 ± 19.2 years. Female patients accounted for 68.9%. MACE occurred in 41.4% of patients during hospitalization. …

Efficacy And Rationale Of Differentiated Racecadotril Doses Combined With Bifidobacterium Triple Viable In Managing Pediatric Persistent Diarrhea: A Comprehensive Analysis, Yanrong Liu, Li Ma, Dian Jin, Jingli Peng, Han Wei, Xueyun Zhang, Yajing Wu

Journal of Pediatric Infectious Diseases

Objective: Pediatric persistent diarrhea poses a severe burden on children's health. This study investigates the efficacy and fundamental mechanisms of varying doses of racecadotril in combination with bifidobacterium triple viable for treating this condition in children. Methods: Employing a randomized controlled trial design, participants diagnosed with pediatric persistent diarrhea were randomly allocated to a high-dose group (observation) and a low-dose group (control), both treated with racecadotril combined with bifidobacterium triple viable. During treatment, clinical parameters were meticulously recorded and analyzed. ELISA techniques were utilized to quantify alterations in immune, inflammatory, and oxidative stress markers in serum. Quantitative culture methods for …

Lncrna Mzf1 Antisense Rna 1 Promotes Mycoplasma Pneumoniae Pneumonia In Children Via Mir-16-5p, Qin Wang, Bing Han, Yanan Ji, Cuicui Zhang, Lianying Ruan, Hengrui Hao

Journal of Pediatric Infectious Diseases

Objective: To determine the expression, as well as the underlying mechanism, of lncRNA MZF1 antisense RNA 1 (MZF1-AS1) in Mycoplasma pneumoniae pneumonia (MPP). Methods: GSE179051 was analyzed for differentially expressed lncRNAs in MP infection. The levels of MZF1-AS1 were determined by quantitative Real-time PCR in children with MPP and healthy subjects. The diagnostic value of MZF1-AS1 was assessed using ROC curves. The effects of MZF1-AS1 knockdown on A549, human primary alveolar macrophages (hpAM) and human airway smooth muscle (ASM) cell function were assessed. The downstream miRNA for MZF1-AS1 was predicted and validated. Results: MZF1-AS1 was a differentially expressed lncRNA in …

Management Of Febrile Infants Under 3 Months Of Age With Sars-Cov-2 Infection In The Emergency Department, Patricia Diego, Aina Seguí, Victoria Trenchs, Carles Luaces, Susanna Hernández-Bou

Journal of Pediatric Infectious Diseases

Objective: Febrile infants with a proven viral infection are at lower risk for a potentially serious bacterial infection (SBI). Our objective is to describe the management of young febrile infants with COVID-19 in the emergency department. Methods: Febrile infants (polymerase chain reaction) test for SARS-CoV-2 between March 2020 and October 2022 were included in a cross-sectional study. The indication for complementary tests, hospital admission and antibiotic therapy was analyzed. We performed a global analysis and by age subgroups (≥29 d). Results: A total of 172 patients were included in the study, out of which 32 (18.6%) were (93%), with urine …

Comparison Of Ischemia-Modified Albumin Levels In Children With Pneumonia And Bronchiolitis, Elife Capan Gun, Feyza Husrevoğlu Esen, Yakup Cag, Yasemin Akın

Journal of Pediatric Infectious Diseases

Objectives: Bronchiolitis and bronchopneumonia are important causes of hospitalization in children. Many biomarkers are used in monitoring the clinical course of these diseases, grading severity, and making hospitalization decisions. This study is therefore intended to evaluate the role of ischemia-modified albumin (IMA) level in the differential diagnosis of pneumonia and bronchiolitis and to compare it with other biomarkers used in the diagnosis. Methods: Thirty patients with pneumonia and thirty patients with bronchiolitis were included in our study. Patients were diagnosed based on history, physical examination, posteroanterior chest radiography, as well as serum C-reactive protein (CRP), sedimentation, procalcitonin (PCT), hemogram, and …

Construction And Validation Of An Early Identification Model For Refractory Mycoplasma Pneumoniae-Positive Lobar Pneumonia, Bi Zhou, Xiaodong Tang, Dawei Mi, Ying Li, Haiyan Liu, Feng Zhu

Journal of Pediatric Infectious Diseases

Objective: This study analyzed the relationship between clinical parameters and prognosis in children with Mycoplasma pneumoniae pneumonia positive lobar pneumonia and developed an early identification model. Methods: Relevant clinical parameters were collected. Patients were then categorized into two groups based on their length of hospital stay: 116 cases in the refractory group (≥10 days) and 94 cases in the non-refractory group (<10 >days). A univariate analysis of variance and binary logistic regression were utilized to develop a predictive model, accompanied by the construction of a nomogram. The model's performance was assessed using ROC curves, diagnostic calibration curves, and DCA curves. …

Neutrophil-To-Lymphocyte Ratio In Neonates: A Predictor Of Early-Onset Neonatal Sepsis, Thi Thanh Binh Nguyen, Khanh Linh Tong, Hoang Mai Linh

Journal of Pediatric Infectious Diseases

Objective: Early-onset neonatal sepsis (EOS) is a common cause of mortality in the neonatal period. The purpose of the study was to determine the value of the neutrophil-to-lymphocyte ratio (NLR) in the early diagnosis of EOS. Methods: This was a prospective cross-sectional study. A total of 102 newborns with early-onset infection (EOI) were enrolled within the first 72 hours of life in the neonatal intensive care unit of a tertiary referral hospital, the largest pediatric center in central Vietnam. Results: Among 102 newborns, 32 were identified as having EOS, while the remaining 70 were classified as EOI. The median NLR …

The Covid-19 Pandemic’S Impact On General Pediatric Ward Admissions: A Single-Centre Study, Ashwaq Aleed, Abdullrahman Alsalamah, Abdulaziz O. Alharbi, Ahmad Alrashedi, Abdullah Al-Nafeesah, Osama Al-Wutayd, Manal A. Al-Batanony

Journal of Pediatric Infectious Diseases

Objective: A decline in healthcare utilisation and hospital admissions resulted from the COVID-19 pandemic, severely affecting many aspects of life. In this study, we sought to evaluate the pandemic impact on general paediatric admissions by comparing the one-year pre-pandemic period. Methods: From the hospital records, all paediatric admissions from the beginning of March 2020 to the end of February 2021 and the corresponding period in 2019–2020 were included in this descriptive study. Results: A total of 4,179 hospitalisations met the study’s inclusion criteria; 2,778 occurred before the pandemic, and 1,401 occurred during the pandemic. The total paediatric hospitalisations decreased by …

Treatment Method Of Hydatid Disease In Children, Which Is A Problem In Developing Countries, Fatma Özcan Sıkı, Mehmet Sarıkaya, Metin Gündüz, Tamer Sekmenli, Alaaddin Nayman, Numan Kılıçlı, İlhan Çiftci

Journal of Pediatric Infectious Diseases

Objective: Hydatid disease (HD) is a parasitic disease caused by ingesting Echinococcus granulosus parasite eggs. HD is a common disease in developing countries. We aimed to evaluate the effectiveness and outcomes of medical, percutaneous, and surgical treatments in pediatric HD cases. Methods: A retrospective review was conducted on pediatric patients diagnosed with HD between January 2013 and January 2023. Data on patients were analyzed. The files of 93 patients who had treatment of 112 hydatid cysts were reviewed. Results: In the study, hydatid cysts were most frequently seen in the liver (65,6%), followed by multiple organ involvement (28.9%) and isolated …

The Impacts Of Fluconazole On Invasive Fungal Infection, Colonization, And Overall Mortality In Preterm Infants: An Updated Systematic Review And Meta-Analysis, Tiehui Huang, Xue Zhang, Xueqiao Lei

Journal of Pediatric Infectious Diseases

Objective: Invasive fungal infection is not uncommon in preterm infants, and fluconazole has been reported to prevent fungal infection. The impacts of fluconazole on invasive fungal infection in preterm infants can be evaluated using an updated systemic review and meta-analysis of randomized clinical trials. Methods: The log risk ratio was transformed using the risk ratio and 95% confidence interval to assess the impacts of fluconazole on fungal infection in preterm infants. After selection, eleven randomized clinical trials of 1097 preterm infants with fluconazole prophylaxis and 953 preterm infants in the control group were enrolled. The focused outcome was the risk …

Biosynthesis Of Metallic Nanoparticles Using Plant Extracts: Mechanisms, Advances, And Sustainable Applications, Alice Njolke Mafe, Great Iruoghene Edo, Patrick Othuke Akpoghelie, Endurance Fegor Isoje, Ufuoma Augustina Igbuku, Joseph Oghenewogaga Owheruo, Arthur Efeoghene Athan Essaghah

Al-Mustaqbal Journal of Sustainability in Engineering Sciences

Growing concerns over the environmental and health impacts of conventional nanoparticle synthesis have driven a shift toward green, plant-based methods. This review highlights the biosynthesis of nanoparticles using plant extracts as a sustainable, economical and non-toxic alternative. Plant-derived phytochemicals such as flavonoids, phenolics, and alkaloids serve dual roles as natural reducing and stabilizing agents, enabling the production of nanoparticles typically ranging between 10 and 100 nm. Several studies have demonstrated up to 80% yield improvement when using optimized extracts from plants like Azadirachta indica and Ocimum sanctum. The review outlines recent progress, including the use of novel botanical sources, …

Chaperone-Usher Pathway Pili Adhesin Conformations And Inhibitors In Urinary Tract Infections, Edward Lopatto

Arts & Sciences Graduate Student Theses and Dissertations

Antibiotic resistant bacteria are becoming increasingly difficult-to-treat resulting in increased morbidity and mortality, especially for common infections such as urinary tract infections (UTIs). Globally, UTIs afflict over 400 million people a year. Numerous different gram-negative and gram-positive bacterial species cause UTIs, including uropathogenic Escherichia coli (UPEC), Klebsiella pneumoniae, and Acinetobacter baumannii. Many of these bacterial species are listed as pathogens of urgent concern by the Center for Disease Control due to the dangerous rise and prevalence of antibiotic resistance necessitating development of new therapeutic measures. Bacteria utilize adhesin factors, such as chaperone-usher pathway (CUP) pili to gain a foothold in …

José Mariano Mociño (1757-1820): Heroic Botanist/Physician Of New And Old Spain, Thomas Milan Janota Wasik

Homage to Alexander von Humboldt: Travels to and from Spain Throughout the Centuries / Homenaje a Alexander von Humboldt: Viajes hacia y desde España de todos los siglos

José Mariano Mociño traveled extensively in New Spain and helped to collect and classify thousands of plants according to modern scientific protocols. In addition, Mociño made significant contributions to science in geology, ethnology, epidemiology, ornithology and horticulture. He was in the vanguard of the study of native Mexican medicinal plants and played a crucial role in the control of infectious disease in the colony and the mother country. Although a contemporary of Alexander von Humboldt, the two never met. Nonetheless, the Prussian traveler was well aware of the accomplishments of Mociño. In his Ensayo politico sobre el Reino de la …