Digital Commons Network^™

Links Between Diversity And Composition Of The Avian Gut Microbiome And Resistance To Experimental Malaria Infection In House Sparrows (Passer Domesticus), Riley Noble

Honors Capstones

No abstract provided.

Evaluation Of Alternative Colony Hybridization Methods For Pathogenic Vibrios, Andrew M. Schwartz, Haley A. Marcotte, Crystal N. Johnson

Faculty Publications

Vibrios, such as Vibrio parahaemolyticus, are naturally occurring halophilic bacteria that are a major cause of foodborne illness. Because of their autochthonous nature, managing vibrio levels in marine and estuarine environments is impossible. Instead, it is crucial to reliably enumerate their abundance to minimize human exposure. One method of achieving this is the direct plating/colony hybridization (DP/CH) method, which has been used to efficiently quantify pathogenic vibrios in oysters and other seafood products. Although successful, the method relies on proprietary resources. We examined alternative approaches, assessed the influence of the reagent suppliers’ source on enumeration accuracy, and made experimental adjustments …

Amino Acid Derivatives Of Chlorin-E6—A Review, Maria Da Graça H. Vicente, Kevin M. Smith

Faculty Publications

Details of the structural elucidation of the clinically useful photodynamic therapy sensitizer NPe6 (15) are presented. NPe6, also designated as Laserphyrin, Talaporfin, and LS-11, is a second-generation photosensitizer derived from chlorophyll-a, currently used in Japan for the treatment of human lung, esophageal, and brain cancers. After the initial misidentification of the structure of this chlorin-e6 aspartic acid conjugate as (13), NMR and other synthetic procedures described herein arrived at the correct structure (15), confirmed using single crystal X-ray crystallography. Interesting new features of chlorin-e6 chemistry (including the intramolecular formation of an anhydride (24)) are reported, allowing chemists to regioselectively conjugate …

Implementation Of A Daily Central Line Maintenance Audit Form In An Outpatient Infusion Center: A Quality Improvement Project, Sarah E. Christiana

DNP Projects

Significance and Background: Best evidence supports central venous access device (CVAD) dressing changes occur every 7 days or sooner, if indicated. At a community hospital in New England, patients receive outpatient antimicrobial therapy via CVADs. It is vital that nurses adequately maintain CVAD dressings according to evidence-based protocols to reduce the risk of central line-associated bloodstream infection (CLABSI). CLABSIs, associated with increased morbidity, mortality, and health care costs, are largely preventable through proper CVAD maintenance. At the project site, a review identified that dressing changes were frequently changed beyond the 7-day parameter. Documentation and surveillance processes were identified as opportunities …

Yale Medicine Magazine Spring 2023, Issue 170, Yale University. School Of Medicine

Yale Medicine Magazine - alumni bulletin and magazine

This is the Spring 2023 issue of Yale Medicine Magazine, issue 170. Prepared in cooperation with the alumni and development offices at the School of Medicine. Earlier volumes are called Yale School of Medicine alumni bulletins, dating from v.1 (1953) through v.13 (1965) and Yale Medicine: alumni bulletin of the School of Medicine, v.1 (1965) through v. 52, no.1. (Autumn 2017).

Pediatric Malaria With Respiratory Distress: Prognostic Significance Of Point-Of-Care Lactate, Catherine Mitran, Robert Opoka, Andrea Conroy, Sophie Namasopo, Kevin Kain, Michael Hawkes

Paediatrics and Child Health, East Africa

Respiratory distress (RD) in pediatric malaria portends a grave prognosis. Lactic acidosis is a biomarker of severe disease. We investigated whether lactate, measured at admission using a handheld device among children hospitalized with malaria and RD, was predictive of subsequent mortality. We performed a pooled analysis of Ugandan children under five years of age hospitalized with malaria and RD from three past studies. In total, 1324 children with malaria and RD (median age 1.4 years, 46% female) from 21 health facilities were included. Median lactate level at admission was 4.6 mmol/L (IQR 2.6–8.5) and 586 patients (44%) had hyperlactatemia (lactate …

Natural Disasters And The Government’S Destructive Response: A Holistic View On The Impacts Of Noncitizen Exclusion From Federal Public Benefit Programs, Faith Zellman

University of Baltimore Law Review

No abstract provided.

Deep Learning Prediction And Its Application In Antimicrobial Resistance, Yiqing Wang

Statistical Science Theses and Dissertations

Antimicrobial Resistance (AMR) is a growing global health threat. It happens when bacteria or other pathogens evolve to resist antibiotics. The relevant infections would cause at least 50,000 deaths yearly in Europe and the United States alone1. To combat the crisis, I developed a data-driven theme for quick and accurate detection of bacterial resistance to a wide range of antibiotics. We utilized statistical approaches and developed a series of novel and rigorous models to tackle the problem of antibiotic resistance.

We proposed a deep learning-based Pan-Antibiotic Resistance Prediction model (PARP). This model enabled AMR prediction for a wide range of …

Impact Of An Evidence-Based Order Set On Pneumonia Length Of Stay, Troy Wayne Spires

Doctor of Nursing Practice Scholarly Projects

Problem Statement: Among adult patients admitted with pneumonia, what is the effect of Computerized Provider Order Entry (CPOE) sets for pneumonia, as compared to usual practice, on the length of stay over three months.

Purpose: The project aimed to determine if inpatient LOS related to pneumonia could be impacted by utilizing appropriately completed computerized physician order entry (CPOE). The current pneumonia order set was evaluated and updated to reflect the latest evidence-based standards of care. Pneumonia order sets with evidence-based practice treatment algorithms was the intervention focus as they have been shown to impact the length of stays …

Spring 2023

La Louisiane

The magazine of the University of Louisiana at Lafayette.

Horizontal Transmission Of Salmonella Typhimurium Among German Cockroaches And Possible Mechanisms, Madison Anne Mond

Honors Thesis

Cockroaches, including the German cockroach (Blattella germanica), are common urban pests that have been linked epidemiologically and experimentally to the transmission of bacteria that cause enteric disease in humans and animals, such as the widespread pathogen Salmonella enterica serovar Typhimurium. While recent strides have been made in understanding the mechanisms and dynamics of Salmonella infection of the cockroach gut, much about this vector-pathogen relationship remains unknown. A particular knowledge gap is understanding the ecology of how S. Typhimurium is acquired, maintained, and spread by cockroaches. The behavioral ecology of German cockroaches is unique among insect vectors. They closely share shelter …

An Exploration Of The Social And Economic Factors That Influence The Mental Health Of Lgbtq College Students, Alexandria Mh Fossum

Senior Theses

LGBTQ individuals face a much higher prevalence of psychiatric disorders and mental health issues than their heterosexual, cisgender counterparts. The added academic pressures of college introduce a unique set of challenges that LGBTQ students face. This thesis includes a review of academic literature and data examining the intersections between social isolation, institutional discrimination, and financial insecurity and the effects they have on the mental health outcomes of LGBTQ college students. The paper is accompanied by a documentary consisting of a set of interviews of LGBTQ college students, giving a more personal insight into the multitude of factors that affect their …

Berberine Disrupts Staphylococcal Proton Motive Force To Cause Potent Anti-Staphylococcal Effects, Neil Zhao, Selin Isguven, Rachel Evans, Thomas P Schaer, Noreen J. Hickok

Department of Orthopaedic Surgery Faculty Papers

The presence of antibiotic resistance has increased the urgency for more effective treatments of bacterial infections. Biofilm formation has complicated this issue as biofilm bacteria become tolerant to antibiotics due to environmental factors such as nutrient deprivation and adhesion. In septic arthritis, a disease with an 11% mortality rate, bacteria in synovial fluid organize into floating, protein-rich, bacterial aggregates (mm-cm) that display depressed metabolism and antibiotic tolerance. In this study, Staphylococcus aureus (S. aureus), which is the most common pathogen in septic arthritis, was tested against different inhibitors that modulate bacterial surface protein availability and that should decrease bacterial aggregation. …

The Economic, Mental Health, And Social Stressors During The Covid-19 Pandemic Among Native Americans In South Dakota, Savannah E. Lukkes

Honors Thesis

American Indian/Alaska Native (AI/AN) communities test positive for COVID-19 and experience higher mortality than other racial and ethnic groups. These high rates of vulnerability could be connected to the systemic inequalities that have been experienced for centuries. This study aimed to compare socioeconomic stressors, concerns, and mental health experiences during the early COVID-19 pandemic between AI/AN and non-AIAN populations in South Dakota. The study sample (n=1,586) was grouped by AI/AN and non-AI/AN status based on self-identification of being AI/AN alone or in combination with another race/ethnicity. Participant responses to socioeconomic stressors and concerns (e.g., top concerns, level of concern, self-isolation, …

Characterization Of Physical And Biochemical Traits In Wheat And Corn Plants Using High Throughput Image Analysis, Kantilata Thapa

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Plant phenotyping has been recognized as a rapidly growing field of research due to the labor-intensive, destructive, and time-consuming nature of traditional phenotyping methods. These phenotyping bottlenecks can be addressed by advancements in image-based phenotyping like RGB and hyperspectral imaging for the assessment of plant traits important for breeding purposes. This study aims (1) to characterize the physical and biochemical traits of wheat and corn plants using RGB and hyperspectral imaging in the greenhouse, and (2) to estimate leaf nitrogen (N), phosphorus (P), and potassium (K) content using hyperspectral imaging and an analytical spectral device (ASD spectrometer) and compare the …

Migrants Sans Papiers: The Impact Of Cantonal Program And Policy Discrepancies On Undocumented Migrant Healthcare Utilization In Switzerland, Denise Peng

Independent Study Project (ISP) Collection

While Switzerland houses what is generally considered one of the best healthcare systems globally, regional policy discrepancies leave undocumented migrants (UDM) in precarious conditions that threaten the nation’s claim to universal health coverage. Critical analyses of existing UDM-catering structures in different cantons, the Swiss Confederation member states, reveal that local discrepancies in healthcare utilization are functional gradients of cantonal support and public endorsement. Relationships with these authorities are instrumental in steering health outcomes not only with program funding and outreach but also management of studies to better understand UDM health determinants and inform future preventative measures.

UDM services and initiatives …

Mir125a As Molecular Target Of Vitamin D Mediated Inhibition Of Cd4+ T Cell Differentiation Into Th17 Cells In Mrl/Lpr Lupus-Prone Mice, Miranda Hilt

Undergraduate Distinction Papers

The dynamic function of vitamin D in an array of immunomodulatory and anti-inflammatory mechanisms and its implication in the pathogenesis of autoimmune diseases has been the topic of much recent scholarship. Here I attempt to elucidate the molecular mechanism of vitamin D-mediated inhibition of the differentiation of CD4+ T cells into pro-inflammatory Th17 cells in MRL/lpr lupus prone mice by investigating how miR-125a expression is affected by dietary modulation of cholecalciferol (vitamin D₃). MRL/MpJ and MRL/lpr mice were split into three experimental groups and fed specially formulated diets that varied in their concentrations of vitamin D. …

Resources For Preparing And Using Antibiograms In Hospital Settings, Fred Tenover, Dana Tawa, Stephanie Mitchell, Isabella A. Tickler, John Stelling, Janet Hindler, Debra A. Goff, Dan Diekema

Training Materials on Antimicrobial Resistance

This is a guide to resources that are available to assist laboratories with the preparation and dissemination of their antimicrobial susceptibility testing data in a format known as an "antibiogram" as an aid to antimicrobial stewardship activities in their hospital. There are three color-coded pathways in the slide deck: Pathway A for laboratories with no experience with antibiograms; Pathway B for laboratories with some experience that want to improve and expand the use of their antibiograms; and Pathway C for experienced laboratories that want to share their data with external surveillance systems.

The Genomics Of Pesticide-Remediating Microbes Relevant To Agricultural Specialty Crops Grown On California’S Central Coast, Kieran Szelong

AES Theses

The overuse of pesticides in agricultural soils has been a long-discussed issue with implications for human health and optimal ecosystem functioning. These compounds may negatively affect non-target species and trophic webs that are otherwise harmless to crops. Various bacteria have been shown to have the capacity to metabolize pesticides, contributing to their remediation from the environment. In order to assess the community context for bacterial remediation of pesticides, we are characterizing soil bacteria communities that exist in agricultural-adjacent soils impacted by pesticide usage across Monterey County, CA.

We collected soil and water samples from fifteen sites exhibiting a range of …

It’S About Time: Rejection Of The De Minimis Doctrine In State Wage And Hour Laws, Abigail Britton

Dickinson Law Review (2017-Present)

Since the passage of the Fair Labor Standards Act (“FLSA”) in 1938, courts have grappled with how to interpret which activities an employee performs for their employer should be considered “work.” The FLSA requires employers pay a minimum wage, pay overtime, and keep records of their employees’ time. However, to calculate these wages based on hours worked, the employer must know what constitutes “work.” Over the 80 years since its enactment, federal courts have adopted rules to determine what counts as work. One doctrine courts apply is the de minimis doctrine. Under the de minimis doctrine, employers do not need …

Smoking Prevalence Amongst People Living With Hiv In Western Jamaica, D Aguilera, B Rinola, S Tundealao, R Klaff, M Aung, M Johnson-Campbell, P Jolly, I Tamí-Maury

GW Research Showcase 2021-2026

No abstract provided.

Covid-19 Drive-Through Testing Facilities: What Have We Learned And What Can Be Improved?, Morgan Burke, Andrew Garret

GW Research Showcase 2021-2026

No abstract provided.

Valacyclovir-Induced Psychosis In An Elderly Esrd Patient With Herpes Zoster, Spencer Carbone, James Croffoot

GW Research Showcase 2021-2026

No abstract provided.

Accuracy Of Therapeutic Drug Monitoring In Vancomycin And The Pharmacist Role: A Retrospective Case-Series, Kateryna Parkhomenko

Senior Theses

Vancomycin is a mainstay of therapy for treating virulent and resistant infections, especially methicillin-resistant Staphylococcus aureus (MRSA). However, vancomycin requires therapeutic drug monitoring (TDM) for optimal dosing and treatment. This requires pharmacists to calculate a dosing regimen that correlates to appropriate goal vancomycin concentrations in the blood. Dosing vancomycin can be difficult, as it varies on a patient’s weight, renal function, age, etc. Doses may have to be adjusted in response to out-of-range concentrations, which requires further pharmacy calculations. Inaccurate dosing poses a risk to patient safety and places a resource and time burden on pharmacists. If vancomycin dosing methods …

Mirna-489 Induces Immunogenic Cell Death In Triple Negative Breast Cancer Cells, Ryan P. Titus

Senior Theses

It has been well established that microRNAs (miRNAs) play an important role in the regulation of gene expression and consequently promoting or downregulating molecular pathways. When dysregulated, miRNAs have been found to serve as important biomarkers for cancer diagnosis and influence tumor initiation and progression. It has been previously established that miRNA-489 is a tumor suppressor microRNA, and it directly targets cell proliferative pathways like the HER2-SHP2-MAPK pathway. In this study, we focus on the role of miRNA-489, in the induction of immunogenic cell death (ICD) in triple-negative breast cancer cell lines. We first examined the effects of miRNA-489 on …

Predicting Grocery Store Visits During The Early Outbreak Of Covid-19 With Machine Learning, Ruijie Bian, Pamela Murray-Tuite, Brian Wolshon

Faculty Publications

While non-essential travel was canceled during the coronavirus infectious disease (COVID-19) pandemic, grocery shopping was essential. The objectives of this study were to: 1) examine how grocery store visits changed during the early outbreak of COVID-19, and 2) estimate a model to predict the change of grocery store visits in the future, within the same phase of the pandemic. The study period (February 15–May 31, 2020) covered the outbreak and phase-one re-opening. Six counties/states in the United States were examined. Grocery store visits (in-store or curbside pickup) increased over 20% when the national emergency was declared on March 13 and …

Rise Of The Metaverse's Immersive Virtual Reality Malware And The Man-In-The-Room Attack & Defenses, Martin Vondráček, Ibrahim Baggili, Peter Casey, Mehdi Mekni

Faculty Publications

The allure of the metaverse along with Virtual Reality (VR) technologies and speed at which they are deployed may shift focus away from security and privacy fundamentals. In this work we employ classic exploitation techniques against cutting edge devices to obtain equally novel results. The unique features of the Virtual Reality landscape set the stage for our primary account of a new attack, the Man-in-the-Room (MitR). This attack, realized from a vulnerable social networking application led to both worming and botnet capabilities being adapted for VR with potential critical impacts affecting millions of users. Our work improves the state-of-the-art in …

Role Of Il-10 In Sars-Cov-2 Infection During Pregnancy, Natalie Lim

Honors Capstones

No abstract provided.

Identifying The Effects Of Anaerobicity And Propionate On Listeria Monocytogenes Metabolism And Central Nervous System Infection, Rebecca Rudd

Honors Theses

Listeria monocytogenes is a facultative foodborne pathogen that can enter the bloodstream and invades the central nervous system to cause meningitis. As an intracellular pathogen, L. monocytogenes replicates inside the host cell cytosol and avoids extracellular immune defenses as it disseminates throughout the body. L. monocytogenes can also reach and cross the blood brain barrier, resulting in severe or fatal symptoms in immunocompromised and elderly patients. The overarching goal of my research project is to better understand how different environmental factors, anaerobicity and propionate, in the intestinal lumen alter the ability of L. monocytogenes to cause infections. In the first …

Genomic Analysis Of Two Phlebotomine Sand Fly Vectors Of Leishmania From The New And Old World, Frédéric Labbé, Maha Abdeladhim, Jenica Abrudan, Alejandra Saori Araki, Ricardo N Araujo, Peter Arensburger, Joshua B Benoit, Reginaldo Pecanha Brazil, Rafaela V Bruno, Gustavo Bueno Da Silva Rivas, Vinicius Carvalho De Abreu, Jason Charamis, Iliano V Coutinho-Abreu, Samara G Da Costa-Latgé, Alistair Darby, Viv M Dillon, Scott J Emrich, Daniela Fernandez-Medina, Nelder Figueiredo Gontijo, Catherine M Flanley, Derek Gatherer, Fernando A Genta, Sandra Gesing, Gloria I Giraldo-Calderón, Bruno Gomes, Eric Roberto Guimaraes Rocha Aguiar, James G C Hamilton, Omar Hamarsheh, Mallory Hawksworth, Jacob M Hendershot, Paul V Hickner, Jean-Luc Imler, Panagiotis Ioannidis, Emily C Jennings, Shaden Kamhawi, Charikleia Karageorgiou, Ryan C Kennedy, Andreas Krueger, José M Latorre-Estivalis, Petros Ligoxygakis, Antonio Carlos A Meireles-Filho, Patrick Minx, Jose Carlos Miranda, Michael J Montague, Ronald J Nowling, Fabiano Oliveira, João Ortigão-Farias, Marcio G Pavan, Marcos Horacio Pereira, Andre Nobrega Pitaluga, Roenick Proveti Olmo, Marcelo Ramalho-Ortigao, José M C Ribeiro, Andrew J Rosendale, Mauricio R V Sant'anna, Steven E Scherer, Nágila F C Secundino, Douglas A Shoue, Caroline Da Silva Moraes, João Silveira Moledo Gesto, Nataly Araujo Souza, Zainulabueddin Syed, Samuel Tadros, Rayane Teles-De-Freitas, Erich L Telleria, Chad Tomlinson, Yara M Traub-Csekö, João Trindade Marques, Zhijian Tu, Maria F Unger, Jesus Valenzuela, Flávia V Ferreira, Karla P V De Oliveira, Felipe M Vigoder, John Vontas, Lihui Wang, Gareth D Weedall, Elyes Zhioua, Stephen Richards, Wesley C Warren, Robert M Waterhouse, Rod J Dillon, Mary Ann Mcdowell

Faculty, Staff and Students Publications

Phlebotomine sand flies are of global significance as important vectors of human disease, transmitting bacterial, viral, and protozoan pathogens, including the kinetoplastid parasites of the genus Leishmania, the causative agents of devastating diseases collectively termed leishmaniasis. More than 40 pathogenic Leishmania species are transmitted to humans by approximately 35 sand fly species in 98 countries with hundreds of millions of people at risk around the world. No approved efficacious vaccine exists for leishmaniasis and available therapeutic drugs are either toxic and/or expensive, or the parasites are becoming resistant to the more recently developed drugs. Therefore, sand fly and/or reservoir control …