Defining The Roles Of Pyruvate Oxidation, Tca Cycle, And Mannitol Metabolism In Methicillin-Resistant Staphylococcus Aureus Catheter-Associated Urinary Tract Infection, Santosh Paudel, Sarah Guedry, Chloe L P Obernuefemann, Scott J. Hultgren, Jennifer N. Walker, Ritwij Kulkarni

2020-Current year OA Pubs

Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of complicated urinary tract infection (UTI) associated with the use of indwelling urinary catheters. Previous reports have revealed host and pathogen effectors critical for MRSA uropathogenesis. Here, we sought to determine the significance of specific metabolic pathways during MRSA UTI. First, we identified four mutants from the Nebraska transposon mutant library in the MRSA JE2 background that grew normally in rich medium but displayed significantly reduced growth in pooled human urine (HU). This prompted us to transduce the uropathogenic MRSA 1369 strain with the transposon mutants in

Osteocalcin Levels Evaluation Of Freeze-Dried Homologus Platelet-Rich Plasma In Periodontal Treatment, Niken Olivia, Kwartarini Murdiastuti

Journal of Dentomaxillofacial Science

Objective: The aim of this study was to evaluate enhanced osteocalcin levels by using freeze-dried homologous platelet-rich plasma (FD HPRP) in periodontal treatment to support the osteogenesis process.

Material and Methods: FD HPRP was made by HPRP from the blood bank, then freeze-drying and for sterilization, the γ-radiation process was carried out with doses of 20 and 25 kGy. The osteoblast (Cell line MG63) was cultured to confluent and then treated with FD HPRP 20 kGy, FD HPRP 25 kGy. Osteocalcin levels were tested using enzyme-linked immunosorbent assay (ELISA) on the 7th and 14th days. The data were analyzed using …

The Impact Of Chatgpt On Student Learning/Performing, M. Samir Abou El-Seoud, Shehab Eldeen Ayman, Khaled A. Nagaty, Omar H. Karam

Computer Science

This paper explores the integration of ChatGPT, an AI-based language model, in undergraduate education. The study examines the potential benefits, challenges, and ethical considerations associated with incorporating ChatGPT into teaching and learning practices. It investigates the impact of ChatGPT on student engagement, critical thinking, and problem-solving skills, as well as its potential to enhance writing skills and provide personalized learning experiences. The survey conducted on faculty staff at a university reveals their perspectives on using ChatGPT, including its effects on learning outcomes and performance. Additionally, the paper addresses concerns related to biases in AI-generated content, privacy issues, copyright considerations, and …

Acute Pseudomembranous Candidiasis Accompanied With Oral Malignant Lesions In Hiv-Infected Patient: Case Report, Dian Angriany, Hendri Susanto, Adiastuti Endah, Bagus Soebadi

Journal of Dentomaxillofacial Science

Objective: Oral candidiasis is the most opportunistic infection in HIV/AIDS patient. Neoplasms may also frequently be found in the oral cavity of HIV/AIDS patient and may indicate the progression of HIV infection. Case Report: A 56-year-old complaint of yellowish-white spots in oral cavity and swelling in the palate accompanied with shortness of breath and difficulty in eating and swallowing. Various medication was used to relieve the complaints but there were no improvements. He claimed a weight loss of 10 kg in last three months. Physically showed pale and weakness. Intraorally, diffuse pseudomembranous was found throughout the oral cavity to pharynx, …

Effectiveness Of Moringa Oleifera Leaf Extract Nanoparticles In Cleaning Smear Layer On The Apical Third Of Root Canal Walls, Juni J. Nugroho, Musthika Jathiasih, Nurhayaty Natsir

Journal of Dentomaxillofacial Science

Objective: To evaluate the cleanliness smear layer on the apical third of the root canal wall in teeth irrigated with 5% and 10% Moringa leaf extract nanoparticles. Method: Vitro experimental laboratory with posttest control group design. A total of 30 mandibular permanent premolars were decorated to an average tooth length of 13 mm. The teeth were divided into 5 groups, with 6 samples per group and each sample was irrigated with 5 ml for 5 minutes. Group I (negative control) irrigated with aquadest steril, group II (positive control) irrigated with NaOCl 5,25%, group III (positive control) irrigated with a …

Dynamics Of Anti-Influenza Mucosal Iga Over A Season In A Cohort Of Individuals Living Or Working In A Long-Term Care Facility, Matt D T Hitchings, Brooke A Borgert, Adam Shir, Bingyi Yang, Kyra H Grantz, Jacob Ball, Carlos A Moreno, Kenneth Rand, Parker A Small, Keith R Fowke, Derek A T Cummings

Faculty, Staff and Student Publications

BACKGROUND: Serological surveys are used to ascertain influenza infection and immunity, but evidence for the utility of mucosal immunoglobulin A (IgA) as a correlate of infection or protection is limited.

METHODS: We performed influenza-like illness (ILI) surveillance on 220 individuals living or working in a retirement community in Gainesville, Florida from January to May 2018, and took pre- and postseason nasal samples of 11 individuals with polymerase chain reaction (PCR)-confirmed influenza infection and 60 randomly selected controls. Mucosal IgA against 10 strains of influenza was measured from nasal samples.

RESULTS: Overall, 28.2% and 11.3% of individuals experienced a 2-fold and …

Automated Processing Of Metabolomics Data For Population-Scale Studies, Ethan Stancliffe

Arts & Sciences Graduate Student Theses and Dissertations

The growth of untargeted metabolomic profiling technology has prompted the upstart of many large cohort studies. Metabolomics serves as an informative complement to other more established ‘omics (e.g., genomics, transcriptomics, proteomics). As a younger discipline, however, experimental and computational workflows are non-standardized and at times inadequate for large-scale studies. Two primary limitations in analysis of untargeted metabolomics data are 1) the poor performance and scalability of the algorithms applied to detect metabolite signals from the raw LC/MS data and 2) inefficient and arduous metabolite identification, which is the process of determining the biochemical structures that correspond to the detected LC/MS …

Class-Driven Synergy And Antagonism Between A Pseudomonas Phage And Antibiotics, Paul Nicholls, Justin R Clark, Carmen Gu Liu, Austen Terwilliger, Anthony W Maresso

Faculty, Staff and Students Publications

The ubiquitous bacterial pathogen Pseudomonas aeruginosa is responsible for severe infections in patients with burns, cystic fibrosis, and neutropenia. Biofilm formation gives physical refuge and a protected microenvironment for sessile cells, rendering cure by antibiotics a challenge. Bacteriophages have evolved to prey on these biofilms over millions of years, using hydrolases and depolymerases to penetrate biofilms and reach cellular targets. Here, we assessed how a newly discovered KMV-like phage (ΦJB10) interacts with antibiotics to treat P. aeruginosa more effectively in both planktonic and biofilm forms. By testing representatives of four classes of antibiotics (cephalosporins, aminoglycosides, fluoroquinolones, and carbapenems), we demonstrated …

Improving Basic Cadet Training For Changing Environment: Case Study At The Korea Air Force Academy, Yoojin Kwon

Electronic Theses and Dissertations, 2020-2023

Increasing uncertainty, such as Covid-19 and weather changes, threatens BCT (Basic Cadet Training) at ROKAFA (Republic of Korea Air Force Academy). In preparation for the changing and adverse environment, this study carried out the following steps to propose a system improvement utilizing LVC (Live, Virtual, Constructive) in BCT system at ROKAFA; 1) Researching the examples of BCT and LVC by advanced countries, 2) Analyzing BCT (ROKAFA) through systems approach (document analysis, survey of 140 cadets and officers, written interviews with military experts), 3) Applying LVC to BCT. BCT is a vital step in the transition from civilian to military status …

Biofilm Detachment As A Passive Method To Survive Bacterial Predation, Alice Wren Goldstein-Plesser

Dartmouth College Master’s Theses

Bacterial biofilms are held together by various, often species specific, secreted proteins, polysaccharides, and DNA – collectively termed the biofilm matrix. Although biofilms have many fitness advantages conferred to them, matrix mutants are abundant within natural isolates, implying context-dependent disadvantages inherent to the production of some matrix components. Here we explore how biofilm architecture controls a tradeoff in the context of different predation pressures. The model organism Escherichia coli is used as a prey species, due to its highly characterized protection against bacteriophages, and the motile bacterial predator Bdellovibrio bacteriovorus as the predatory exposure. We use microfluidics paired with confocal …

Compositions And Methods For Modulating The Endothelial Barrier, Jerome William Breslin, Zeinab Yehia Khalil Motawe

USF Patents

Disclosed are compositions and methods for modulating endothelial barrier function.

Examining The Impact Of Covid-19 On Elementary-Aged Minoritized Students, Ashlynn Ramirez

Electronic Theses and Dissertations, 2020-2023

The Coronavirus Disease [COVID-19] pandemic was a public health crisis. As of January 2022, there were over 314 million infections and over 5.5 million deaths (Assefa et al., 2022). Unfortunately, COVID-19 disproportionately impacted minoritized populations. This study will investigate the relationship between COVID-19 and the impact on minoritized students attending Title I elementary schools in one urban school district. According to Lopez-Ibor (2006), "disasters are diverse events as a consequence of a danger that affects social groups and produces material and human losses resulting in insufficient resources of the community and insufficient coping through social mechanisms" (p. 22). COVID-19 affected …

Assessing The Barriers To Reintegration Among Returnees In Nigeria, Itunu Ilesanmi

Electronic Theses and Dissertations, 2020-2023

Reintegration for internally displaced returnees in Nigeria needs improvement due to the barrier returnees encounter when returning to their communities of origin. As a path to recovery following the unsettling experiences of generalized violence caused by Boko Haram terrorist attacks in Northeastern Nigeria, there is a need for best practices to mitigate challenges during reintegration for internally displaced returnees. With an estimated 2.5 million internally displaced persons (IDPs) in Northeastern Nigeria at the end of 2022, 1.98 million have returned to their prior residence, where they must cope and survive while attempting to reintegrate without effective and efficient support to …

Evaluation Of Different Stocking Densities On Fattening Performance, Slaughter, Carcass, Meat Quality Characteristics, And Fatty Acid Composition In New Zealand Rabbits, Büşra Yaranoğlu, Mustafa Hi̇lmi̇ Yaranoğlu, Sali̇ha Uysal, Adnan Adi̇l Hi̇şmi̇oğullari

Turkish Journal of Veterinary & Animal Sciences

The aim of the research was to investigate the effects of different stocking densities on fattening performance, slaughter, carcass, meat quality characteristics, and fatty acid composition in New Zealand rabbits raised in cage conditions and also determine the ideal housing frequency so that yields were not adversely affected. In the study, 36 New Zealand rabbits were used. After weaning, the fattening was started and rabbits were put into cages at different stocking densities as 1 rabbit/cage, 2 rabbits/cage, and 3 rabbits/ cage. The live weight of rabbits and feed consumption data were taken every week. At the end of 8 …

Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron

Turkish Journal of Veterinary & Animal Sciences

Mastitis is a contagious disease that causes significant milk loss and serious economic damage in dairy animals. Streptococcus agalactiae causes clinical and subclinical mastitis in animals and infections in humans, especially adults, and newborns. This study aimed to confirm the identification of S. agalactiae isolates by PCR in buffalo milk; to determine the antibiotic resistance and virulence genes by multiplex PCR. The material of this study consisted of 30 (12%) S. agalactiae strains identified by phenotypic, biochemical, and PCR methods (rRNA-16S) from 250 isolates with bacterial growth from milk samples of buffaloes with mastitis. In addition, the Kirby-Bauer disc diffusion …

Comparison Of Bacterial Profile And Antibiotic Susceptibility Isolated From Surgical Site After Ventral Midline And Lateral Flank Approaches Of Ovariohysterectomy In Queens And Bitches, Ebru Karakaya Bi̇len, Serpi̇l Kahya Demi̇rbi̇lek, Öznur Yilmaz, Muhammet Ali̇ Karadağ, Havva Aner

Turkish Journal of Veterinary & Animal Sciences

The aim of the study was to identify the bacterial profile and antimicrobial susceptibility from surgical site cultures in queens and bitches undergoing ovariohysterectomy via ventral midline and lateral flank approaches. Healthy 22 queens and 21 bitches were assigned randomly either ventral midline or lateral flank approach for routine ovariohysterectomy. Surgical site samples were collected before the surgery and on the 1$^{st}$, 2$^{nd}$ , and 3 $^{rd}$days after the surgery for microbiological analysis. A total of 70 different strains were isolated from 50 (29%) of 172 samples. In both queens and bitches the total number of bacteria isolated from the …

In Vitro Antimicrobial And Antibiofilm-Forming Activities Of Origanum Munzurense Plant Extract On Some Foodborne Gram-Negative Bacteria, Hali̇l Yalçin, Esra Zeybek, Oktay Özkan, Asim Kart

Turkish Journal of Veterinary & Animal Sciences

lants are an important source for the discovery of antimicrobial formulations due to the many biologically active components they contain. In this study, in vitro antimicrobial and antibiofilm forming activities of ethanolic extract of Origanum munzurense were investigated against some strains of foodborne pathogenic bacteria. To investigate the antimicrobial activity, the minimal inhibitory concentration values against the selected bacteria and antibiofilm-forming activity were tested with the liquid microdilution method and microplate method, respectively. MICs of Origanum munzurense extract against the bacteria tested are 32 mg/mL for Escherichia coli O157: H7 ATCC 35150, 32 mg/mL for Escherichia coli O157: H7 ATCC …

Global Serum Profiling: An Opportunity For Earlier Cancer Detection., Alexandra Sala, James M. Cameron, Paul M. Brennan, Emma J. Crosbie, Tom Curran, Ewan Gray, Pierre Martin-Hirsch, David S. Palmer, Ihtesham U. Rehman, Nicholas J W Rattray, Matthew J. Baker

Manuscripts, Articles, Book Chapters and Other Papers

The advances in cancer research achieved in the last 50 years have been remarkable and have provided a deeper knowledge of this disease in many of its conceptual and biochemical aspects. From viewing a tumor as a 'simple' aggregate of mutant cells and focusing on detecting key cell changes leading to the tumorigenesis, the understanding of cancer has broadened to consider it as a complex organ interacting with its close and far surroundings through tumor and non-tumor cells, metabolic mechanisms, and immune processes. Metabolism and the immune system have been linked to tumorigenesis and malignancy progression along with cancer-specific genetic …

Severe Covid-19 Following Rituximab And Nirmatrelvir/Ritonavir Treatment In A Patient With Mctd, Case Report, Rohail A. Baig, Faieja Chowdhury, Omar Siddiqui, Adnaan Sheikh, Varun Mehta, Pranjali Dakwale

Advances in Clinical Medical Research and Healthcare Delivery

This is an article about how the use of medications such as nirmatrelvir/ritonavir can cause rebound COVID-19 and how the use of Rituximab, a biologic agent, can prolong the duration and increase severity of symptoms of COVID-19 in patients with pre-disposed autoimmune diseases that are on chronic pharmacotherapy.

Iy-1 Perspectives, V. 1 - Complete Issue, Iy-1 Perspectives

iY-1 Perspectives

No abstract provided.

Exploring The Use Of Artificial Intelligence To Achieve Sustainable Development Goal #3: Affordable Healthcare In Impoverished Countries, Qianyu Wang

iY-1 Perspectives

No abstract provided.

Mhealth And Sustainable Development: The Case Of Rural China, Zhenpeng (Joe) Liao

iY-1 Perspectives

No abstract provided.

The Role Of Librarians In Curbing Monkey Pox In Nigeria, Helen Famaren Tor-Akwer Cln, Dominic Aondohemba Ihongo Cln, Gabriel Iortyom Ajav Cln

Library Philosophy and Practice (e-journal)

The paper focuses on Influence of Librarians on curbing Monkey pox in Nigeria, it examines the concept of monkey pox and a brief history of it and how it got into Nigeria. Signs and symptom of monkey pox were also discussed as well as the mode of transmission which basically is through animals and from an infected person to the other. The paper further shows discussed the influence of librarians in curbing monkey pox in Nigeria. Four research objectives and four research questions were formulated for the study. Random sampling technique will be used to draw 250 health draws from …

High-Throughput Bioprinting Of The Nasal Epithelium Using Patient-Derived Nasal Epithelial Cells., I Deniz Derman, Miji Yeo, Diana Cadena, Megan Callender, Mian Horvath, Zengshuo Mo, Ruoyun Xiong, Elizabeth Fleming, Phylip Chen, Mark E Peeples, Karolina Palucka, Julia Oh, Ibrahim T Ozbolat

Faculty Research 2023

Progenitor human nasal epithelial cells (hNECs) are an essential cell source for the reconstruction of the respiratory pseudostratified columnar epithelium composed of multiple cell types in the context of infection studies and disease modeling. Hitherto, manual seeding has been the dominant method for creating nasal epithelial tissue models through biofabrication. However, this approach has limitations in terms of achieving the intricate three-dimensional (3D) structure of the natural nasal epithelium. 3D bioprinting has been utilized to reconstruct various epithelial tissue models, such as cutaneous, intestinal, alveolar, and bronchial epithelium, but there has been no attempt to use of 3D bioprinting technologies …

A Fate Determination Fork-In-The-Road For Germinal Center Tfh And T Memory Cells, Jeff Hansen

Browse All News

No abstract provided.

Immunological Monitoring In Hepatitis C Virus Controlled Human Infection Model, Naglaa H Shoukry, Andrea L Cox, Christopher M Walker

Faculty, Staff and Student Publications

Controlled human infection model trials for hepatitis C virus represent an important opportunity to identify correlates of protective immunity against a well-characterized inoculum of hepatitis C virus and how such responses are modified by vaccination. In this article, we discuss the approach to immunological monitoring during such trials, including a set of recommendations for optimal sampling schedule and preferred immunological assays to examine the different arms of the immune response. We recommend that this approach be adapted to different trial designs. Finally, we discuss how these studies can provide surrogate predictors of the success of candidate vaccines.

Predicting Pediatric Tuberculosis: The Need For Age-Specific Host Biosignatures, Sara Suliman, Devan Jaganath, Andrew Dinardo

Faculty, Staff and Students Publications

No abstract provided.

Socio-Economic Determinants Of Gender-Based Violence [Gbv]: Sdg Analytics On The Global Gbv Scenario With Special Reference To Technology Facilitated Gender-Based Violence And Adolescent Birth Rates, Chandra P. Daniel

NYMC Student Theses and Dissertations

Gender-based violence (GBV) is a multifaceted problem. The most rapidly increasing modern form of violence is the intertwined epidemic of ‘Technology Facilitated GBV’ [TF GBV] and sexual violence against women and girls. It was critical to investigate the global impact of this epidemic during COVID-19 since a disproportionate impact of violence was observed among women and girls in low and middle-income countries. A retrospective cross-sectional research design was adopted using linear regression analysis (univariate and multivariate) on SDG 5.2, a set of global indicators, to elicit the socioeconomic determinants of GBV. Phase-I results exposed the top-four socio-economic determinants …

Enhancing The Performance Of Microbial Fuel Cells Via Metabolic Engineering Of Escherichia Coli For Phenazine Production, Olja Simoska, Dale A. Cummings, Erin M. Gaffney, Claire Langue, Tommy G. Primo, Courtney J. Weber, Corbin E. Witt, Shelley D. Minteer

Chemistry Faculty Research & Creative Works

One of the central roles in the design, development, and application advances in mediator-Based microbial electrochemical systems, such as microbial fuel cells (MFCs), is the establishment of efficient and successful communication between conductive electrode surfaces and microorganisms via modes of extracellular electron transfer (EET). Most microbial-Based systems require the use of artificial electroactive mediators in order to facilitate and/or enhance electron transfer. Our previous work established an exogenous phenazine-Based library as a mediator system to enable EET from the model microorganism Escherichia coli as a promising biotechnological host. However, the addition of exogenous mediators to a microbial electrochemical system has …

Bimanual Movement Characteristics And Real-World Performance Following Hand-Arm Bimanual Intensive Therapy In Children With Unilateral Cerebral Palsy, Shailesh S. Gardas, Christine Lysaght, Amy Gross Mcmillan, Shailesh Kantak, John D. Willson, Charity G. Patterson, Swati M. Surkar

Department of Rehabilitation Medicine Faculty Papers

The purpose of this study was to quantify characteristics of bimanual movement intensity during 30 h of hand-arm bimanual intensive therapy (HABIT) and bimanual performance (activities and participation) in real-world settings using accelerometers in children with unilateral cerebral palsy (UCP). Twenty-five children with UCP participated in a 30 h HABIT program. Data were collected from bilateral wrist-worn accelerometers during 30 h of HABIT to quantify the movement intensity and three days pre- and post-HABIT to assess real-world performance gains. Movement intensity and performance gains were measured using six standard accelerometer-derived variables. Bimanual capacity (body function and activities) was assessed using …