Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

2023

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 5491 - 5520 of 25067

Full-Text Articles in Entire DC Network

Synlight: A Bicistronic Strategy For Simultaneous Active Zone And Cell Labeling In The Drosophila Nervous System, Michael A. Aimino, Jesse Humenik, Michael J. Parisi, Juan Carlos Duhart, Timothy J. Mosca Sep 2023

Synlight: A Bicistronic Strategy For Simultaneous Active Zone And Cell Labeling In The Drosophila Nervous System, Michael A. Aimino, Jesse Humenik, Michael J. Parisi, Juan Carlos Duhart, Timothy J. Mosca

Farber Institute for Neuroscience Faculty Papers

At synapses, chemical neurotransmission mediates the exchange of information between neurons, leading to complex movement, behaviors, and stimulus processing. The immense number and variety of neurons within the nervous system make discerning individual neuron populations difficult, necessitating the development of advanced neuronal labeling techniques. In Drosophila, Bruchpilot-Short and mCD8-GFP, which label presynaptic active zones and neuronal membranes, respectively, have been widely used to study synapse development and organization. This labeling is often achieved via the expression of 2 independent constructs by a single binary expression system, but expression can weaken when multiple transgenes are expressed by a single driver. Recent …

Go to article

Managing Solar Energy Development To Balance Private Property Rights And Consumer Protection With The Protection Of Land And Communities, Tennessee. Advisory Commission On Intergovernmental Relations. Sep 2023

Managing Solar Energy Development To Balance Private Property Rights And Consumer Protection With The Protection Of Land And Communities, Tennessee. Advisory Commission On Intergovernmental Relations.

Water and Utilities Reports

No abstract provided.

Go to article

The Lumberjack, September 27, 2023 Sep 2023

The Lumberjack, September 27, 2023

Cal Poly Humboldt's Student Newspaper

The student newspaper of Humboldt State University.

Go to article

Methamphetamine-Induced Region-Specific Transcriptomic And Epigenetic Changes In The Brain Of Male Rats, Benpeng Miao, Xiaoyun Xing, Viktoriia Bazylianska, Pamela Madden, Anna Moszczynska, Bo Zhang Sep 2023

Methamphetamine-Induced Region-Specific Transcriptomic And Epigenetic Changes In The Brain Of Male Rats, Benpeng Miao, Xiaoyun Xing, Viktoriia Bazylianska, Pamela Madden, Anna Moszczynska, Bo Zhang

2020-Current year OA Pubs

Psychostimulant methamphetamine (METH) is neurotoxic to the brain and, therefore, its misuse leads to neurological and psychiatric disorders. The gene regulatory network (GRN) response to neurotoxic METH binge remains unclear in most brain regions. Here we examined the effects of binge METH on the GRN in the nucleus accumbens, dentate gyrus, Ammon's horn, and subventricular zone in male rats. At 24 h after METH, ~16% of genes displayed altered expression and over a quarter of previously open chromatin regions - parts of the genome where genes are typically active - showed shifts in their accessibility. Intriguingly, most changes were unique …

Go to article

September 27, 2023, The Dakota Student Sep 2023

September 27, 2023, The Dakota Student

The Dakota Student

No abstract provided.

Go to article

Wind Symphony Sep 2023

Wind Symphony

Bailey School of Music Concert Programs

Program for the Wind Symphony performance on September 27, 2023

Go to article

Writing And Resisting Colonial Genocide, Heidi Matthews, Luann Good Gingrich, Joel Ong Sep 2023

Writing And Resisting Colonial Genocide, Heidi Matthews, Luann Good Gingrich, Joel Ong

Articles & Book Chapters

Canada has pursued policies of Indigenous assimilation and annihilation, many of which continue today. Among others, these include ‘Indian residential schools’, the Indian Act, welfare-state child removals, the Sixties Scoop, the prohibition of cultural practices, forced sterilization and environmental destruction. We are scholars co-leading a large interdisciplinary programme of research studying ‘colonial genocide’. Our research seeks to understand how historic colonialism and its contemporary manifestations rely on genocidal logic for power and profit. While we begin in Turtle Island, our work has global application. The act of naming is a powerful analytical and political tool, and ‘genocide’ is one of …

Go to article

Independent- Sep. 27, 2023, Leslie Lozada Sep 2023

Independent- Sep. 27, 2023, Leslie Lozada

The Independent (1988-present)

No abstract provided.

Go to article

Self-Organization For Community Resilience In An Invisible Agricultural Community, Anna Erwin, Chelsea A. Silva, Zhao Ma Sep 2023

Self-Organization For Community Resilience In An Invisible Agricultural Community, Anna Erwin, Chelsea A. Silva, Zhao Ma

School of Earth, Environmental, & Marine Sciences Faculty Publications

This study investigates how self-organizing efforts by residents of informal settlements, primarily migrant and informal farmworkers, shape community resilience in Majes, a water-scarce irrigation district in the Atacama Desert of Peru. We collected 45 semi-structured interviews with residents and authorities in Majes and analyzed findings through a framework of self-organizing. Analyses revealed that self-organizing by residents of informal settlements incorporated the three components of White’s theory of Community Agency and Community Resilience, which contends that marginalized communities increase resilience by fostering a commons praxis, practicing a prefigurative politics, and developing opportunities for economic autonomy. We also found …

Go to article

Repurposing Normal Chromosomal Microarray Data To Harbor Genetic Insights Into Congenital Heart Disease, Nephi Walton, Hoang Nguyen, Sara Procknow, Darren Johnson, Alexander Anzelmi, Patrick Jay Sep 2023

Repurposing Normal Chromosomal Microarray Data To Harbor Genetic Insights Into Congenital Heart Disease, Nephi Walton, Hoang Nguyen, Sara Procknow, Darren Johnson, Alexander Anzelmi, Patrick Jay

Department of Medicine Faculty Papers

About 15% of congenital heart disease (CHD) patients have a known pathogenic copy number variant. The majority of their chromosomal microarray (CMA) tests are deemed normal. Diagnostic interpretation typically ignores microdeletions smaller than 100 kb. We hypothesized that unreported microdeletions are enriched for CHD genes. We analyzed "normal" CMAs of 1762 patients who were evaluated at a pediatric referral center, of which 319 (18%) had CHD. Using CMAs from monozygotic twins or replicates from the same individual, we established a size threshold based on probe count for the reproducible detection of small microdeletions. Genes in the microdeletions were sequentially filtered …

Go to article

Separation Anxiety: Filming The Nicosia Buffer Zone, K Parker, S Moore Sep 2023

Separation Anxiety: Filming The Nicosia Buffer Zone, K Parker, S Moore

School of Art, Design and Architecture

Published in the first study on empty places in photography and the Covid-19 pandemic, Parker’s chapter examines the cinematographic depiction of ‘emptiness’ in the author’s site-specific essay film, Father-land (2018), made collaboratively with the film-maker Stuart Moore through an artists’ research residency in Nicosia, the only divided capital city in Europe. It details the process-based research methods that led to Father-land’s affective depiction of the politically-charged, ‘empty landscape’ of the Nicosia Buffer Zone, the demilitarized strip of land monitored by the United Nations, which has separated the Greek Cypriot south of Cyprus from the Turkish-occupied north since the violent conflict …

Go to article

2023-09-27 Death Of Tokitae: The Impact Of Orca Captivity, Tyra Okuda Sep 2023

2023-09-27 Death Of Tokitae: The Impact Of Orca Captivity, Tyra Okuda

The Spectator Online

No abstract provided.

Go to article

Office Of Research, Economic Engagement And Outreach Update, September 27, 2023, Office Of Research, Economic Engagement And Outreach Sep 2023

Office Of Research, Economic Engagement And Outreach Update, September 27, 2023, Office Of Research, Economic Engagement And Outreach

Office of Research, Economic Engagement and Outreach

No abstract provided.

Go to article

Differential Effects Of Pancreatic Cancer-Derived Extracellular Vesicles Driving A Suppressive Environment, Anurag Purushothaman, Jacqueline Oliva-Ramírez, Warapen Treekitkarnmongkol, Deivendran Sankaran, Mark W Hurd, Anirban Maitra, Subrata Sen Sep 2023

Differential Effects Of Pancreatic Cancer-Derived Extracellular Vesicles Driving A Suppressive Environment, Anurag Purushothaman, Jacqueline Oliva-Ramírez, Warapen Treekitkarnmongkol, Deivendran Sankaran, Mark W Hurd, Anirban Maitra, Subrata Sen

Faculty, Staff and Student Publications

Pancreatic ductal adenocarcinoma (PDAC) cells display extensive crosstalk with their surrounding environment to regulate tumor growth, immune evasion, and metastasis. Recent advances have attributed many of these interactions to intercellular communication mediated by small extracellular vesicles (sEVs), involving cancer-associated fibroblasts (CAF). To explore the impact of sEVs on monocyte lineage transition as well as the expression of checkpoint receptors and activation markers, peripheral blood monocytes from healthy subjects were exposed to PDAC-derived sEVs. Additionally, to analyze the role of sEV-associated HA in immune regulation and tissue-resident fibroblasts, monocytes and pancreatic stellate cells were cultured in the presence of PDAC sEVs …

Go to article

Kernel Density Estimation Of Conditional Distributions To Detect Responses In Satellite Tag Data, Joshua Hewitt, Alan E. Gelfand, Nicola J. Quick, William R. Cioffi, Brandon L. Southall, Stacy L. Deruiter, Robert S. Schick Sep 2023

Kernel Density Estimation Of Conditional Distributions To Detect Responses In Satellite Tag Data, Joshua Hewitt, Alan E. Gelfand, Nicola J. Quick, William R. Cioffi, Brandon L. Southall, Stacy L. Deruiter, Robert S. Schick

School of Biological and Marine Sciences

Background As levels of anthropogenic noise in the marine environment rise, it is crucial to quantify potential associated effects on marine mammals. Yet measuring responses is challenging because most species spend the majority of their time submerged. Consequently, much of their sub-surface behavior is difficult or impossible to observe and it can be difficult to determine if—during or following an exposure to sound—an observed dive differs from previously recorded dives. We propose a method for initial assessment of potential behavioral responses observed during controlled exposure experiments (CEEs), in which animals are intentionally exposed to anthropogenic sound sources. To identify possible …

Go to article

Propofol Affords No Protection Against Delayed Cerebral Ischemia In A Mouse Model Of Subarachnoid Hemorrhage, Meizi Liu, Keshav Jayaraman, James W Nelson, Jogender Mehla, Deepti Diwan, Ananth K Vellimana, Gregory J Zipfel, Umeshkumar Athiraman Sep 2023

Propofol Affords No Protection Against Delayed Cerebral Ischemia In A Mouse Model Of Subarachnoid Hemorrhage, Meizi Liu, Keshav Jayaraman, James W Nelson, Jogender Mehla, Deepti Diwan, Ananth K Vellimana, Gregory J Zipfel, Umeshkumar Athiraman

2020-Current year OA Pubs

Delayed cerebral ischemia (DCI) is an important contributor to poor outcomes in aneurysmal subarachnoid hemorrhage (SAH) patients. We previously showed that volatile anesthetics such as isoflurane, sevoflurane and desflurane provided robust protection against SAH-induced DCI, but the impact of a more commonly used intravenous anesthetic agent, propofol, is not known. The goal of our current study is to examine the neurovascular protective effects of propofol on SAH-induced DCI. Twelve-week-old male wild-type mice were utilized for the study. Mice underwent endovascular perforation SAH or sham surgery followed one hour later by propofol infusion through the internal jugular vein (2 mg/kg/min continuous …

Go to article

Buncombe Street Administrative Board Minutes 1996-2000, Buncombe Street United Methodist Church Sep 2023

Buncombe Street Administrative Board Minutes 1996-2000, Buncombe Street United Methodist Church

Methodist Local Church Records

No abstract provided.

Go to article

Spartan Daily, September 26, 2023, San Jose State University, School Of Journalism And Mass Communications Sep 2023

Spartan Daily, September 26, 2023, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2023

Volume 161, Issue 15

Go to article

Comparative Skull Morphology Of California Sea Lions (Zalophus Californianus) And Steller Sea Lions (Eumetopias Jubatus) In The Pacific Northwest And Implications For Hybridization, Ada Iris Garcia Garcia Sep 2023

Comparative Skull Morphology Of California Sea Lions (Zalophus Californianus) And Steller Sea Lions (Eumetopias Jubatus) In The Pacific Northwest And Implications For Hybridization, Ada Iris Garcia Garcia

Dissertations and Theses

Eumetopias jubatus (Steller sea lions) that breed on the Oregon coast are smaller than those found both in Alaska and California. During the breeding season, male Zalophus californianus (California sea lions) move past E. jubatus rookeries in Oregon on their way to their breeding rookeries in Southern California. Introgression potentially could explain the difference in size of Oregon E. jubatus relative to remaining populations. At Portland State University (PSU), the marine mammal collection of the Museum of Vertebrate Biology has over 200 skulls of Z. californianus and E. jubatus from throughout northern Oregon and southern Washington. To assess whether morphologically …

Go to article

Putative Photosensitivity In Internal Light Organs (Organs Of Pesta) Of Deep-Sea Sergestid Shrimps, Tamara Frank, Jamie Sickles, Danielle M. Deleo, Patricia Blackwelder, Heather Bracken-Grissom Sep 2023

Putative Photosensitivity In Internal Light Organs (Organs Of Pesta) Of Deep-Sea Sergestid Shrimps, Tamara Frank, Jamie Sickles, Danielle M. Deleo, Patricia Blackwelder, Heather Bracken-Grissom

Marine & Environmental Sciences Faculty Articles

Many marine species can regulate the intensity of bioluminescence from their ventral photophores in order to counterilluminate, a camoufage technique whereby animals closely match the intensity of the downwelling illumination blocked by their bodies, thereby hiding their silhouettes. Recent studies on autogenic cuticular photophores in deep-sea shrimps indicate that the photophores themselves are light sensitive. Here, our results suggest photosensitivity in a second type of autogenic photophore, the internal organs of Pesta, found in deep-sea sergestid shrimps. Experiments were conducted onboard ship on live specimens, exposing the animals to bright light, which resulted in ultrastructural changes that matched those seen …

Go to article

Intrinsic Tumor Resistance To Car T Cells Is A Dynamic Transcriptional State That Is Exploitable With Low-Dose Radiation, Alexander B Kim, Ssu-Yu Chou, Solomon Kang, Eric Kwon, Matthew Inkman, Jeff Szymanski, Neal Andruska, Cian Colgan, Jin Zhang, Joanna C Yang, Nathan Singh, Carl J Deselm Sep 2023

Intrinsic Tumor Resistance To Car T Cells Is A Dynamic Transcriptional State That Is Exploitable With Low-Dose Radiation, Alexander B Kim, Ssu-Yu Chou, Solomon Kang, Eric Kwon, Matthew Inkman, Jeff Szymanski, Neal Andruska, Cian Colgan, Jin Zhang, Joanna C Yang, Nathan Singh, Carl J Deselm

2020-Current year OA Pubs

Chimeric antigen receptor (CAR) T-cell therapy represents a major advancement for hematologic malignancies, with some patients achieving long-term remission. However, the majority of treated patients still die of their disease. A consistent predictor of response is tumor quantity, wherein a higher disease burden before CAR T-cell therapy portends a worse prognosis. Focal radiation to bulky sites of the disease can decrease tumor quantity before CAR T-cell therapy, but whether this strategy improves survival is unknown. We find that substantially reducing systemic tumor quantity using high-dose radiation to areas of bulky disease, which is commonly done clinically, is less impactful on …

Go to article

Exogenous Ketone Supplements For Reducing Anxiety-Related Behavior, Csilla Ari D'Agostino, Dominic Paul D'Agostino Sep 2023

Exogenous Ketone Supplements For Reducing Anxiety-Related Behavior, Csilla Ari D'Agostino, Dominic Paul D'Agostino

USF Patents

Methods of treating anxiety disorders or reducing anxiety-related behaviors. The methods include administering a therapeutically effective amount of ketone supplementation, such as butanediol, ketone esters (e.g., 1,3-butanediol-acetoacetate diester) and/or ketone salts (e.g., beta-hydroxybutyrate-mineral salt), chronically, sub-chronically, or acutely, with or without admixture with a medium chain triglyceride or in combination. It was determined herein that ketone supplementation reduced anxiety in rats on elevated plus maze as measured by less entries to closed arms, more time spent in open arms, more distance travelled in open arms, and delayed latency to entrance to closed arms, when compared to control. Along with reducing …

Go to article

Galloylated Procyanidins For Treating Endovascular Dysfunction And Increasing Blood Flow, Michael Louis Sperduti, Matthew Cleary Nickerson, Jimmy Roy Huddleston, Leigh Anthony West Sep 2023

Galloylated Procyanidins For Treating Endovascular Dysfunction And Increasing Blood Flow, Michael Louis Sperduti, Matthew Cleary Nickerson, Jimmy Roy Huddleston, Leigh Anthony West

USF Patents

The present disclosure is directed to compositions, methods of use, and methods of making, wherein the compositions contain one or more galloylated procyanidins having between two and five epicatechin monomers, wherein the one or more galloylated procyanidins include epicatechin-(4-8)-epicatechin-(4-8)-epicatechin-gallate, catechin trimer gallate, catechin dimer digallate, or combinations thereof.

Go to article

Nicotinic Receptor Antagonists And Pioglitazone As Therapeutic Agents For Covid-19, Paul Ronald Sanberg, Christian Bernard Brechot, Shyam S. Mohapatra, Subhra Mohapatra Sep 2023

Nicotinic Receptor Antagonists And Pioglitazone As Therapeutic Agents For Covid-19, Paul Ronald Sanberg, Christian Bernard Brechot, Shyam S. Mohapatra, Subhra Mohapatra

USF Patents

A method of treating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), otherwise known as COVID-19, infection by administering nicotinic receptor antagonists and/or PPAR-γ agonists is presented. A combination of mecamylamine, or an isomer thereof, and pioglitazone synergistically reduces replication of SARS-CoV-2 in virus-infected cells and also synergistically reduces inflammatory cytokines such as IL-6, IL-β, and TNFα, which have been associated with SARS-CoV-2 infection.

Go to article

Using Multiple Lenses To See An Invisible Group, Kathleen J. Farkas, Jaroslaw Richard Romaniuk Sep 2023

Using Multiple Lenses To See An Invisible Group, Kathleen J. Farkas, Jaroslaw Richard Romaniuk

Faculty Scholarship

Social work in Poland and in the United States shares the values of human dignity and self-determination, but there are often value conflicts in terms of how various groups experience social roles and social expectations. This paper explores the use of multiple lenses to understand the past and current conditions for LGBT+ people in Poland. An international, university-level collaboration uses a framework of “invisible groups” to highlight the needs of those who are on the margins of society and whose human and individual rights are suppressed. The article reviews the results of a recently published on-line survey of LGBT+ populations …

Go to article

Absence Of Chordin-Like 1 Aids Motor Recovery In A Mouse Model Of Stroke, Eileen Collyer, Bridget R Boyle, Yolanda Gomez-Galvez, Lorraine Iacovitti, Elena Blanco-Suarez Sep 2023

Absence Of Chordin-Like 1 Aids Motor Recovery In A Mouse Model Of Stroke, Eileen Collyer, Bridget R Boyle, Yolanda Gomez-Galvez, Lorraine Iacovitti, Elena Blanco-Suarez

Farber Institute for Neuroscience Faculty Papers

Chordin-like 1 (Chrdl1) is an astrocyte-secreted protein that regulates synaptic maturation, and limits plasticity via GluA2-containing AMPA receptors (AMPARs). It was demonstrated that Chrdl1 expression is very heterogeneous throughout the brain, and it is enriched in astrocytes in cortical layers 2/3, with peak expression in the visual cortex at postnatal day 14. In response to ischemic stroke, Chrdl1 is upregulated during the acute and sub-acute phases in the peri-infarct region, potentially hindering recovery after stroke. Here, we used photothrombosis to model ischemic stroke in the motor cortex of adult male and female mice. In this study, we demonstrate that elimination …

Go to article

Evaluation Of Phytochemicals And The Role Of Oxidative Stress Pathways During Fruit Development In Strawberries (Fragaria×Ananassa), Fahimeh Mokhtari Shojaee, Elham Mohajel Kazemi, Houshang Nosrati, Maryam Kolahi, Mehdi Haghi Sep 2023

Evaluation Of Phytochemicals And The Role Of Oxidative Stress Pathways During Fruit Development In Strawberries (Fragaria×Ananassa), Fahimeh Mokhtari Shojaee, Elham Mohajel Kazemi, Houshang Nosrati, Maryam Kolahi, Mehdi Haghi

Turkish Journal of Botany

Strawberries (Fragaria × ananassa) are one of the Rosaceae family, considered economically and nutritionally important berry fruits. Phytochemicals are crucial ingredients that contribute to characteristics such as the fruit?s aroma and flavor. The current study investigated the phytochemical characteristics of strawberry fruit at three developmental stages (green, white, and red). HPLC measurements determined the glucose, fructose, and sucrose concentrations significantly increased in the red fruit, whereas glucose was identified as the major sugar in ripe fruit (2.8 g.g-1FW). Evaluation of the phenylpropanoid pathway implied that the amount of total phenol and flavonoid following phenylalanine ammonia-lyase enzyme activity decreased …

Go to article

Pet Imaging Of Microglia Using Pbr28suv Determines Therapeutic Efficacy Of Autologous Bone Marrow Mononuclear Cells Therapy In Traumatic Brain Injury, Supinder S Bedi, Michael C Scott, Max A Skibber, Akshita Kumar, Henry W Caplan, Hasen Xue, David Sequeira, Alison L Speer, Fanni Cardenas, Franciska Gudenkauf, Karen Uray, Amit K. Srivastava, Alan R Prossin, Charles S Cox Sep 2023

Pet Imaging Of Microglia Using Pbr28suv Determines Therapeutic Efficacy Of Autologous Bone Marrow Mononuclear Cells Therapy In Traumatic Brain Injury, Supinder S Bedi, Michael C Scott, Max A Skibber, Akshita Kumar, Henry W Caplan, Hasen Xue, David Sequeira, Alison L Speer, Fanni Cardenas, Franciska Gudenkauf, Karen Uray, Amit K. Srivastava, Alan R Prossin, Charles S Cox

Cardeza Foundation for Hematologic Research

Traumatic brain injury (TBI) results in activated microglia. Activated microglia can be measured in vivo by using positron emission topography (PET) ligand peripheral benzodiazepine receptor standardized uptake values (PBR28suv). Cell based therapies have utilized autologous bone marrow mononuclear cells (BMMNCs) to attenuate activated microglia after TBI. This study aims to utilize in vivo PBR28suv to assess the efficacy of BMMNCs therapy after TBI. Seventy-two hours after CCI injury, BMMNCs were harvested from the tibia and injected via tail-vein at 74 h after injury at a concentration of 2 million cells per kilogram of body weight. There were three groups of …

Go to article

In Vivo Crispr/Cas9 Screening Identifies Pbrm1 As A Regulator Of Myeloid Leukemia Development In Mice, Qian Zhu, Davide Seruggia, Yuko Fujiwara, Christopher R Vakoc, Stuart H Orkin Sep 2023

In Vivo Crispr/Cas9 Screening Identifies Pbrm1 As A Regulator Of Myeloid Leukemia Development In Mice, Qian Zhu, Davide Seruggia, Yuko Fujiwara, Christopher R Vakoc, Stuart H Orkin

Faculty, Staff and Students Publications

CRISPR/Cas9 screening approaches are powerful tool for identifying in vivo cancer dependencies. Hematopoietic malignancies are genetically complex disorders in which the sequential acquisition of somatic mutations generates clonal diversity. Over time, additional cooperating mutations may drive disease progression. Using an in vivo pooled gene editing screen of epigenetic factors in primary murine hematopoietic stem and progenitor cells (HSPCs), we sought to uncover unrecognized genes that contribute to leukemia progression. We, first, modeled myeloid leukemia in mice by functionally abrogating both Tet2 and Tet3 in HSPCs, followed by transplantation. We, then, performed pooled CRISPR/Cas9 editing of genes encoding epigenetic factors and …

Go to article

Tmem27 Suppresses Tumor Development By Promoting Ret Ubiquitination, Positioning, And Degradation, Gabriela Huelgas-Morales, Jonathan Lefkowitz, Wan Song, Bethany N Landry, Hector Lopez, Cynthia M Estrada-Zuniga, Shivi Goyal, Mohammad Aasif Khan, Timothy J Walker, Exing Wang, Faqian Li, Yanli Ding, Lois M Mulligan, Ricardo C T Aguiar, Patricia L M Dahia Sep 2023

Tmem27 Suppresses Tumor Development By Promoting Ret Ubiquitination, Positioning, And Degradation, Gabriela Huelgas-Morales, Jonathan Lefkowitz, Wan Song, Bethany N Landry, Hector Lopez, Cynthia M Estrada-Zuniga, Shivi Goyal, Mohammad Aasif Khan, Timothy J Walker, Exing Wang, Faqian Li, Yanli Ding, Lois M Mulligan, Ricardo C T Aguiar, Patricia L M Dahia

Faculty, Staff and Student Publications

The TMEM127 gene encodes a transmembrane protein of poorly known function that is mutated in pheochromocytomas, neural crest-derived tumors of adrenomedullary cells. Here, we report that, at single-nucleus resolution, TMEM127-mutant tumors share precursor cells and transcription regulatory elements with pheochromocytomas carrying mutations of the tyrosine kinase receptor RET. Additionally, TMEM127-mutant pheochromocytomas, human cells, and mouse knockout models of TMEM127 accumulate RET and increase its signaling. TMEM127 contributes to RET cellular positioning, trafficking, and lysosome-mediated degradation. Mechanistically, TMEM127 binds to RET and recruits the NEDD4 E3 ubiquitin ligase for RET ubiquitination and degradation via TMEM127 C-terminal PxxY motifs. Lastly, increased cell …

Go to article

First Prev Next Last