Digital Commons Network^™

Sally Baskin Hooker: Reminiscences, Barbara Wingo

The Marjorie Kinnan Rawlings Journal of Florida Literature

No abstract provided.

Speleogenomics Of Cave Life In North America, Joseph B. Benito

Dissertations

Cave organisms with their unusual morphologies including loss of eyes, lack of body pigmentation, and the compensatory improvement of nonvisual sensory systems have long intrigued and fascinated biologists. Due to their restricted distributions and life history traits, many troglomorphic (subterranean-adapted) species are considered imperiled and are high priority targets for protective management. With increasing anthropogenic threats to cave ecosystems, it is important to study cave-dwelling organisms, so that informed management decisions can be made on a regular basis. Detailed information on the genetics, taxonomy, distribution, and colonization history of cavernicoles are necessary to make rigorous ecological inference and develop respective …

Assessment Of Opioid-Induced Immunomodulation In Experimental And Clinical Sepsis, Brittany Dawn Bissell, Jamie L. Sturgill, Maria E. C. Bruno, Erick D. Lewis, Marlene E. Starr

Markey Cancer Center Faculty Publications

CONTEXT: Opioids remain a standard supportive therapy in patients admitted to the ICU with sepsis. However, as preclinical models indicate an association between opioid exposure and immunosuppression, the use of this class of drugs warrants investigation. The objective of this study was to investigate whether opioid exposure causes immunosuppression in patients with sepsis, and to use a murine sepsis model to determine the effects of opioid exposure on secondary infection.

HYPOTHESIS: We hypothesized opioid exposure would be associated with immunosuppression in patients with sepsis and secondary infection in a murine sepsis model.

METHODS AND MODELS: This was a two-phase preclinical …

Bilirubin Levels Are Negatively Correlated With Adiposity In Obese Men And Women, And Its Catabolized Product, Urobilin, Is Positively Associated With Insulin Resistance, Zachary A. Kipp, Mei Xu, Evelyn A. Bates, Wang-Hsin Lee, Philip A. Kern, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Bilirubin levels in obese humans and rodents have been shown to be lower than in their lean counterparts. Some studies have proposed that the glucuronyl UGT1A1 enzyme that clears bilirubin from the blood increases in the liver with obesity. UGT1A1 clearance of bilirubin allows more conjugated bilirubin to enter the intestine, where it is catabolized into urobilin, which can be then absorbed via the hepatic portal vein. We hypothesized that when bilirubin levels are decreased, the urobilin increases in the plasma of obese humans, as compared to lean humans. To test this, we measured plasma levels of bilirubin and urobilin, …

Polycomb Deficiency Drives A Foxp2-High Aggressive State Targetable By Epigenetic Inhibitors, Fan Chen, Aria Byrd, Jinpeng Liu, Robert M. Flight, Tanner Ducote, Kassandra J. Naughton, Xiulong Song, Abigail R Edgin, Alexsandr Lukyanchuk, Danielle T. Dixon, Christian M. Gosser, Dave-Preston Esoe, Rani Jayswal, Stuart H. Orkin, Hunter N. B. Moseley, Chi Wang, Christine Fillmore Brainson

Markey Cancer Center Faculty Publications

Inhibitors of the Polycomb Repressive Complex 2 (PRC2) histone methyltransferase EZH2 are approved for certain cancers, but realizing their wider utility relies upon understanding PRC2 biology in each cancer system. Using a genetic model to delete Ezh2 in KRAS-driven lung adenocarcinomas, we observed that Ezh2 haplo-insufficient tumors were less lethal and lower grade than Ezh2 fully-insufficient tumors, which were poorly differentiated and metastatic. Using three-dimensional cultures and in vivo experiments, we determined that EZH2-deficient tumors were vulnerable to H3K27 demethylase or BET inhibitors. PRC2 loss/inhibition led to de-repression of FOXP2, a transcription factor that promotes migration and stemness, and FOXP2 …

Suppressing Hepatic Ugt1a1 Increases Plasma Bilirubin, Lowers Plasma Urobilin, Reorganizes Kinase Signaling Pathways And Lipid Species And Improves Fatty Liver Disease, Evelyn A. Bates, Zachary A. Kipp, Genesee J. Martinez, Olufunto O. Badmus, Mangala M. Soundarapandian, Donald Foster, Mei Xu, Justin F. Creeden, Jennifer R. Greer, Andrew J. Morris, David E. Stec, Terry D. Hinds Jr.

Markey Cancer Center Faculty Publications

Several population studies have observed lower serum bilirubin levels in patients with non-alcoholic fatty liver disease (NAFLD). Yet, treatments to target this metabolic phenotype have not been explored. Therefore, we designed an N-Acetylgalactosamine (GalNAc) labeled RNAi to target the enzyme that clears bilirubin from the blood, the UGT1A1 glucuronyl enzyme (GNUR). In this study, male C57BL/6J mice were fed a high-fat diet (HFD, 60%) for 30 weeks to induce NAFLD and were treated subcutaneously with GNUR or sham (CTRL) once weekly for six weeks while continuing the HFD. The results show that GNUR treatments significantly raised plasma bilirubin levels and …

Xylazine Suppresses Fentanyl Consumption During Self-Administration And Induces A Unique Sex-Specific Withdrawal Syndrome That Is Not Altered By Naloxone In Rats, Shailesh N. Khatri, Safiyah M. Sadek, Percell T. Kendrick, Emma O. Bondy, Mei Hong, Sally Pauss, Dan Luo, Thomas E. Prisinzano, Kelly E. Dunn, Julie A. Marusich, Joshua S. Beckmann, Terry D. Hinds Jr., Cassandra D. Gipson

Markey Cancer Center Faculty Publications

Prescription and illicit opioid use are a public health crisis, with the landscape shifting to fentanyl use. Since fentanyl is 100-fold more potent than morphine, its use is associated with a higher risk of fatal overdose that can be remediated through naloxone (Narcan) administration. However, recent reports indicate that xylazine, an anesthetic, is increasingly detected in accidental fentanyl overdose deaths. Anecdotal reports suggest that xylazine may prolong the fentanyl “high,” alter the onset of fentanyl withdrawal, and increase resistance to naloxone-induced reversal of overdose. To date, no preclinical studies have evaluated the impacts of xylazine on fentanyl self-administration (SA; 2.5 …

Driving Simulator : Driving Performance Under Distraction, Kaushik Pilligundla

Master's Projects

This pilot study used a driving simulator experiment to look into how podcast consumption affects driving performance as a continuous distraction. Three volunteers conducted three trials in the study, each with a different driving scenario. Data analysis was done to compare two conditions. The first condition is the Audio, where volunteers listen to podcasts while driving. The second condition is no-audio condition.. The no-audi condition had nothing to play in the background. We used eye-tracking technology to gather gaze data. The study's findings using the post survey and eye fixation data indicate that listening to podcasts leads to continuous distraction …

Nitrogen Behavior In A No-Tillage Agroecosystem Located In The Inner Bluegrass Of Kentucky, Alec William Besinger Mr.

Theses and Dissertations--Plant and Soil Sciences

Nitrogen (N) is a crucial element, often limiting productivity in ecosystems. This limitation is relieved N fertilizer additions. No-tillage (NT) agroecosystems are prone to lower N availability translating to lower crop yields. The default explanation for lower N availability in NT is microbial immobilization of added N fertilizer. Historically, N availability is often assessed using chemical extractions such as KCl to evaluate levels of inorganic N in the soil. The possibility of this abiotic clay mineral fixation as mechanism in well-drained NT soils has been overlooked. The objectives of this study are to quantify N availability using KCl, NaCl, and …

Comparison Of Botanical Composition Methods And Change Over Time In Kentucky Pastures, Echo Elizabeth Gotsick

Theses and Dissertations--Plant and Soil Sciences

Botanical composition of pastures has been measured with numerous methods over the last century, but there have been limited direct comparisons between methods. The objective of this study was to compare botanical composition methods, to determine the most accurate and efficient method, and to access pasture composition change over time. Six farms with two pastures each were monitored across the state of Kentucky. Sampling occurred fall 2020 through fall 2022, three times a year using the following methods: step point, visual estimation, occupancy grid, and point quadrat (used as a reference method). The occupancy grid showed the highest similarity to …

The Under-Enforcement Of Crimes Against Black Women, Lisa Avalos

Journal Articles

It is well known that over-policing has a severe adverse impact on communities of color. What is less well known is that over-policing is accompanied by a corollary—a pervasive and systemic under-policing of violence against women of color. The refusal to see women of color as victims of crime who are worthy recipients of justice, and to minimize the severity of violence committed against them, are habits that are deeply embedded in the American system of [in]justice. From an 1855 Supreme Court decision refusing to recognize a female slave’s right to sexual autonomy to a prosecutor’s 2021 decision to prosecute …

Carbon Capture And Storage: Models For Compensating Holdout Landowners, Keith B. Hall

Journal Articles

No abstract provided.

Paradigm Shift, Expansion, And Inclusion: Visual Literacy Research For The Field Of Information, Yan Ma

Graduate School of Library and Information Studies Faculty Publications

In addition to the continuing call for the inclusion of visual literacy into the curriculum for library and information science education, a paradigm expansion in research to develop and lead an interdisciplinary research advancement is a pressing task. The proposed paradigm shift in research results from the shift in the nature of information from text-based to visual information. Such a paradigm shift requires expanding research methodologies and theoretical frameworks. At the same time, paradigm inclusion is needed to invite and embrace interdisciplinary research and diverse research methodologies and theoretical frameworks to study visual information. This paradigm shift, expansion, and inclusion …

Interlink Between The Gut Microbiota And Inflammation In The Context Of Oxidative Stress In Alzheimer’S Disease Progression, Tushar K Das, Bhanu P Ganesh

Faculty, Staff and Student Publications

The microbiota-gut-brain axis is an important pathway of communication and may dynamically contribute to Alzheimer's disease (AD) pathogenesis. Pathological commensal gut microbiota alterations, termed as dysbiosis, can influence intestinal permeability and break the blood-brain barrier which may trigger AD pathogenesis via redox signaling, neuronal, immune, and metabolic pathways. Dysbiosis increases the oxidative stress. Oxidants affect the innate immune system through recognizing microbial-derived pathogens by Toll-like receptors and initiating the inflammatory process. Most of the gut microbiome research work highlights the relationship between the gut microbiota and AD, but the contributory connection between precise bacteria and brain dysfunction in AD pathology …

Is Patient Controlled Analgesia A Feasible Method Of Pain Relief In The Emergency Department: A Mixed Method Study, Raja Natasya Raja Azlan

Theses

Although pain is the most common reason for patients to present to emergency departments (EDs), conventional methods of pain management in EDs are suboptimal. Patient controlled analgesia (PCA), commonly used for post operative pain relief, was proposed as a modality of pain management in ED. PCA enables the patient to self-administer intravenous analgesia, has been rigorously evaluated in acute care settings and is widely used in many clinical areas. It is not routinely used in EDs. The literature review found critical gaps in the existing evidence of PCA use in EDs justifying this research. A three-phase sequential mixed methods study …

Novel Investigations For Reflux Disease And Their Complications In The Oesophagus, Jin-Soo Park

Theses

Gastro-oesophageal reflux disease (GORD) is a disease process in which gastric acid from the stomach refluxes upwards into the lower oesophagus and causes typical symptoms such as heartburn. In contrast, laryngopharyngeal reflux disease (LPR) is a reflux disease where stomach contents reflux upward to the upper oesophagus, pharynx, larynx, and lungs. LPR instead causes more nebulous and atypical symptoms such as voice change, chronic cough, and laryngitis. Furthermore, prolonged acid contact can cause cellular changes that increase risk of oesophageal malignancy.

GORD is a prevalent disease, and there are various investigative modalities to assess, diagnose, and quantify its impacts. On …

Enforcing Soft Law In International Investment Arbitration, Vera Korzun

Vanderbilt Journal of Transnational Law

Drawing examples from international environmental law, sustainable development, and corporate social responsibility, this Article examines the evolving role of international investment arbitration in the enforcement of non-binding soft law rules of international law. In doing so, the Article explains how investment tribunals can, and have been called upon to, interpret and, paradoxically, enforce soft law instruments. The Article calls for reevaluation of the nature of soft law and the role of investor-state dispute settlement in international rulemaking and enforcement. It also argues that for international environmental law and law on sustainable development, where the lack of an enforcement mechanism has …

Physiologically-Based Pharmacokinetic (Pbpk) Modeling For The Preclinical Development Of Spectinamide Antibiotics, Keyur R. Parmar

Theses and Dissertations (ETD)

Despite being an ancient disease caused by Mycobacterium tuberculosis (Mtb) and after decades of research, tuberculosis (TB) still affects millions of people every year worldwide. In 2018, the World Health Organization (WHO) reported that 10 million people developed tuberculosis and 1.5 million died of the disease. With the increase in the multidrug-resistant (MDR) and extensively drug-resistant (XDR) cases, the treatment for TB with the standard first and second-line therapy is becoming increasingly difficult. Therefore, there is an urgent need to find new anti-TB drugs as combination partners of existing and experimental classes of antimicrobial agents to shorten and simplify the …

Strafford County 2022 Annual Report Of The Commissioners, Treasurer, Other County Officers, And The Strafford County Delegation For The Year Ending December 31, 2022., Strafford County Representatives

Strafford County, NH Annual Reports

This is an annual report containing vital statistics for a county in the state of New Hampshire.

Keep Working Hands On Working Land, Elyse Caiazzo

Graduate Student Portfolios, Professional Papers, and Capstone Projects

Strategies for Effective Farmland Transitions, Succession Planning, and Landowner Education

Co-Op To Cafeteria: Building A Food Value Chain For Farm To School, Blake D. Lineweaver

Graduate Student Portfolios, Professional Papers, and Capstone Projects

The central theme of this M.S. Environmental Studies portfolio considers farm-to-school as a medium for food system transformation within an innovative regional partnership of producer-owned food hubs and a state education agency. The portfolio consists of three main elements.

The first element is a literature review that examines the holistic farm-to-school model, with a particular focus on local food procurement.

The second element is a strategic communications plan, which was developed as part of an internship project with the Northwest Food Hub Network and the Mission Mountain Food Enterprise Center. The aim of this plan is to promote value-added products …

Examining The Role Of Place Attachment In Climate Justice Engagement And Jewish Relationships To The Environment, Madeline Medina

Environmental Studies Honors Projects

It is critical that environmental justice and marginalized identities are the focus of climate-related discussions and research. Solutions must support the long-term wellbeing of people, especially and importantly those who are most vulnerable to the consequences of climate change. Psychological research suggests that place attachment–the meaningful bonds that occur between people and their environment (Scannell & Gifford, 2010)–is a key factor in motivating environmental behavior, but little research has examined its connection to environmental justice oriented behavior. This two-part exploration first evaluated the role of place attachment on engagement with both a typical climate change centered message and a climate …

Unmet: The Undergraduate Experience Of Basic Need Insecurity At An Uncommon University, Michelle C. Bair, Rachelle V. Blackwell-Stegall, Sara E. Rafuse, Ra’Meer J. Roberts, Sara E. Weinstein

Doctor of Education Capstones

Virginia Commonwealth University’s (VCU) Dean of Students office submitted a Request for Assistance with needs related to food and housing insecurity and lack of basic funds for students. To address this request, a doctoral Capstone team conducted a problem and context analysis, literature review, student focus groups, and an internal survey of departments. The goal was to explore the undergraduate experience of unmet needs and to identify gaps and overlaps in basic needs support and services already provided at VCU. Findings suggest that students do not know the resources available to them through the Dean of Students office, that students …

To Determine The Role Of The Platelet Activating Factor - Receptor In Folfirinox Therapy-Mediated Microvesicles Particle Generation, Krishna Awasthi

Browse all Theses and Dissertations

Given that Pancreatic cancer continues to be one of the primary causes of cancer-related death and that existing treatment options have a poor success rate, it is important to investigate the role of potential factor(s) involved in pancreatic cancer growth or diminishing the effectiveness of chemotherapeutic agents. An alternative regimen for pancreatic cancer treatment called FOLFIRINOX has shown to increase the survival rate and improve Eastern Cooperative Oncology Group (ECOG) performance status score among the pancreatic cancer patients. However, FOLFIRINOX therapy is associated with several side effects. Through mechanisms involving the secretion of microvesicle particles (MVP), studies, including ours have …

Exploring The Relationship Between The Epigenome And Metabolism In Mycn-Amplified Neuroblastoma, Krista M. Dalton

Theses and Dissertations

High risk neuroblastoma (NB) is responsible for nearly 15% of all cancer related deaths in the pediatric population. The transcription factor MYCN is the most studied oncogene in NB, automatically conferring high-risk and has been found to be difficult to target. One way that MYCN amplification in NB functions to support tumorigenicity is through altered metabolism, satisfying the demand for rapid proliferation by increasing the nutrient flux through biosynthetic pathways. We performed an unbiased screen on select metabolic targeted therapy combinations and correlated sensitivity with over 20 subsets of cancer. We found that MYCN-amplified NB is hypersensitive to the …

Advancing Social Equity And Promoting Black Self-Determination: A Community Garden Opportunity Plan For Richmond, Va, Alessandro U. Ragazzi

Master of Urban and Regional Planning Capstone Projects

Urban agriculture provides a variety of economic, environmental, and social benefits. Recognizing these benefits, the City of Richmond administers a community garden program known as “Richmond Grows Gardens.” Through this program, underutilized city properties are permitted for use as community gardens. However, an emerging concern is the potential for community gardens to perpetuate underlying structures of social and racial inequality through displacement and social exclusion. Considering these concerns, this plan investigates the implementation of community gardens in Richmond and establishes a methodology for ranking existing and available community garden sites based on the social and racial demographics of their surrounding …

Developing Agrihoods: The Context For Petersburg, Brandon L. Archer

Master of Urban and Regional Planning Capstone Projects

Petersburg has an extensive history of navigating through challenging times. From its inception, African Americans have played an important role in the development of the city, and today that spirit is still present. As evidenced by an extensive network of community support and stakeholders, the Petersburg Youth Farm has matured into a resource for not just the residents of the neighborhood but of the city as a whole. By incorporating some innovative planning techniques and building a housing development truly dedicated to community needs, we can improve lived experiences in the city.

By adopting the recommendations set forth in this …

Trolley Line Trail Creative Placemaking Plan, Courtland Caldwell

Master of Urban and Regional Planning Capstone Projects

The Trolley Line Trail is a planned multi-use trail along the historic Richmond-to-Ashland Trolley corridor. The purpose of this plan is to identify potential creative placemaking locations and installations to highlight the trolley's unique past and present. This plan sets forth a vision for creative placemaking by gathering community feedback, analyzing properties along the Trolley Line Trail, and making recommendations for placemaking and public art. Ultimately, the recommendations laid out in this plan aim to preserve and acknowledge the community's unique identity while creating a safe, accessible experience for all users.

Windmill Ridge Park: A Sustainable Park Design, Sean Benson

Master of Urban and Regional Planning Capstone Projects

This plan proposes designing and developing a new park in Chesterfield County that will promote sustainability in its design and implementation, as well as provide a stepping stone to promote these practices throughout Chesterfield County parks. This plan achieves this by providing research, concepts, and recommendations centered on the three Es of sustainability: environmental quality, social equity, and economic efficiency.

Predicting Mountain Lion Resource Selection And Abundance In North America, William Connor O'Malley

Graduate Student Theses, Dissertations, & Professional Papers

The relationship between habitat quality and density is well documented in lower trophic levels but to what extent it can be extended to higher trophic levels is unknown. I tested the relationship between habitat quality, home-range size and density using a wide-ranging, well-studied, top carnivore, the mountain lion (Puma concolor). First, I created a second-order resource selection function (RSF) for mountain lions in their current North American range using GPS collar data from 476 individuals in 20 study sites and remotely-sensed landscape data. I used the RSF and home range estimates derived from collared animals to quantify mountain …