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2023

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Ultrasound-Assisted Extraction Of Cannabis Sativa And Biological Activities Assessments For Dermatological Application, Pannita Kongtananeti Jan 2023

Ultrasound-Assisted Extraction Of Cannabis Sativa And Biological Activities Assessments For Dermatological Application, Pannita Kongtananeti

Chulalongkorn University Theses and Dissertations (Chula ETD)

Cannabis sativa L. is a medicinal plant that has long been used in Thai traditional medicine and is still widely used today, with many uses in pharmaceuticals, including the treatment of skin infections. It contains a complex mixture of secondary metabolites, including cannabinoids and non-cannabinoid-type constituents, especially detla-9-tetrahydrocannabinol (THC), cannabidiol (CBD), and terpenes. However, fewer studies of cannabis have been reported regarding its anti-aging potential by investigating tyrosinase inhibition activity. In this study, ultrasound-assisted extraction (UAE) of 5 different parts of Cannabis sativa L., the determination of bioactive compounds using GC–MS/MS and the assessment of their biological properties related to …

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Contamination Of Antimicrobial-Resistant Escherichia Coli, Salmonella Spp., Enterococcus Faecalis, And Enterococcus Faecium In Commercial Raw Meat-Based Diets For Cats And Dogs, Rusmin Indra Jan 2023

Contamination Of Antimicrobial-Resistant Escherichia Coli, Salmonella Spp., Enterococcus Faecalis, And Enterococcus Faecium In Commercial Raw Meat-Based Diets For Cats And Dogs, Rusmin Indra

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study aimed to investigate the contamination of third-generation cephalosporin (3GC)-resistant Escherichia coli, Salmonella spp., Enterococcus faecalis, and E. faecium in commercial raw meat-based diets (RMBDs) for cats and dogs in Thailand. Fifty RMBD products from ten brands were included for total plate count and bacterial isolation. Antimicrobial susceptibility testing was conducted for the targeted bacteria. Extended-spectrum β-lactamase (ESBL) production and the presence of blaCTX-M and mcr genes were detected. Additionally, E. coli phylogroups and Salmonella serovars were identified. The RMBD samples were contaminated by 3GC-resistant E. coli (80.0%), Salmonella spp. (70.0%), E. faecalis (100%), and E. faecium (84.0%). All …

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National Survey Of Human Epigenetic Study In Healthy Aged Population In Four Different Regions Of Thailand, Siwaporn Nilyai Jan 2023

National Survey Of Human Epigenetic Study In Healthy Aged Population In Four Different Regions Of Thailand, Siwaporn Nilyai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Healthy ageing has become a goal to help older people maintain good-quality longevity. Researchers have found that epigenetic, environmental, and behavioural lifestyle factors influence the variation of lifespan and healthspan. This study aims to evaluate the age-associated epigenetic biomarkers among older people living in four major regions of Thailand, along with their association with lifestyle and sociodemographic characteristics. 214 participants passed the criteria and enrolled from the southern, northeastern, central, and northern areas of Thailand. Sociodemographic characteristics were recorded through interviews, and blood samples were collected to evaluate blood chemistry and epigenetic markers. SIRT1 and HMGB1 proteins in plasma were …

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Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit Jan 2023

Volatile Organic Compound Profiles Identifiled In Vaginal Discharge Of Bitches During Estrous Cycle, Amornthep Preechatangkit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Volatile organic compounds (VOCs) are the metabolites of microorganisms. VOCs are low molecular weight and easily evaporate at room temperature, all odorants are VOC. VOCs are different depending on health status and reproductive status. The objective of this study was to profile the VOCs in bitches during estrous cycle, and to compare VOCs between stages of estrous cycle and between pre-ovulatory, ovulation and post-ovulatory periods. A total of 23 normal, healthy bitches requested for ovulation timing as part of the routine breeding management were used in this study. All animals had a previous history of normal estrous cycle without any …

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Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem Jan 2023

Reproductive Performance Of Gilts And Sows In Gestation Group Housing And Free Farrowing System In Tropical Climate, Natchanon Dumniem

Chulalongkorn University Theses and Dissertations (Chula ETD)

Animal welfare is a rising topic in the swine industry, resulting in the adoption of alternative housing systems. However, the knowledge of management practices to mitigate the deleterious effects of these systems on reproductive performance in tropical environments is limited. This thesis aims to determine the impacts of alternative housing systems on the reproductive performance of sows and gilts, comprising 4 parts. i) To compare reproductive performance among conventional crates (CC), group housing gestation pen with temporary farrowing crate (PC), and group housing gestation pen and free-farrowing pen (PP). On average, the farrowing rate of sows in the CC group …

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Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit Jan 2023

Analytical Performance Of Progesterone Testing Using Surface Plasmon Field-Enhanced Fluorescence Spectroscopy Technology In Dogs, Pimuk Sungprasit

Chulalongkorn University Theses and Dissertations (Chula ETD)

A new clinical chemistry analyzer using surface plasmon field-enhanced fluorescence spectroscopy (SPFS) has been introduced into veterinary diagnostic laboratories as a point-of-care unit, enabling rapid turnaround times for both diagnosis and follow-up. Consequently, the test results must be accurate, precise, and reliable. This research aims to evaluate the method performance of the analyzer. The evaluation was conducted according to Clinical Laboratory Improvement Amendments (CLIA) guidelines using method validation. The performance evaluation included studies on imprecision (within-day and between-day), method comparison, recovery tests, and linearity for determining progesterone concentration. The method validation results showed good and acceptable precision. Serum spiking concentrations …

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Metagenomic Analysis Using Oxford Nanopore For Fungal Identification In Hemoculture Sample, Nattapong Langsiri Jan 2023

Metagenomic Analysis Using Oxford Nanopore For Fungal Identification In Hemoculture Sample, Nattapong Langsiri

Chulalongkorn University Theses and Dissertations (Chula ETD)

Currently, with advancements in medical technology, the mortality rate from fungal infections has also increased, particularly since the late 20th century. The incidence of invasive fungal infections, especially bloodstream fungal infections, is rising. However, laboratory diagnosis of fungi from clinical specimens still faces significant limitations. Diagnosing fungi based on morphological characteristics to identify species level can take weeks, which is not aligned with the severity of the disease. Current molecular biology techniques also have limitations, such as PCR success rates and mixed infections. With significant advancements in sequencing technology leading to third-generation sequencing, there are notable benefits. Third-generation sequencing, or …

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Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa Jan 2023

Genetic Diversity Of Influenza A Viruses In Live Bird Markets And Free-Grazing Ducks In Thailand, Supanat Boonyapisitsopa

Chulalongkorn University Theses and Dissertations (Chula ETD)

Influenza A viruses (IAVs) can infect birds and a wide variety of mammals. Live bird markets (LBMs) and free-grazing ducks (FGDs) are the important reservoirs of influenza A viruses in Thailand. This study aims to gain the overall information of IAVs circulated in LBMs in Bangkok and in FGD flocks in Thailand. IAVs surveillance in LBM in Bangkok was conducted to identified the IAVs in LBM during 2019-2020. The IAV-H9N2 (n=8) and IAV-H9Nx (n=2) were isolated from 1,178 swab samples. The IAVs of the previous CU-EIDAs’ LBM surveillance programs during 2014-2018 were included for overall genetic analysis. Total 6 subtypes …

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Molecular Investigation And Genetic Characterization Of Canine Astrovirus And Canine Kobuvirus With Associated Pathology In Domestic Dogs In Vietnam And Thailand, Tin Van Nguyen Jan 2023

Molecular Investigation And Genetic Characterization Of Canine Astrovirus And Canine Kobuvirus With Associated Pathology In Domestic Dogs In Vietnam And Thailand, Tin Van Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Canine astrovirus (CaAstV) and canine kobuvirus (CaKoV) are recently recognized pathogens associated with canine gastrointestinal disease (GID), though their exact role remains poorly understood. In both Vietnam and Thailand, these viruses were detected in dogs with and without diarrhea, particularly high frequency in puppies under 6 months old. Co-infection between CaAstV and CaKoV, as well as with other common canine enteric viruses (canine parvovirus, canine enteric coronavirus, canine distemper virus), were also confirmed. Complete coding sequences (CDS) of 7 CaAstV strains and 9 CaKoV strains from Vietnam, and 2 CaAstV strains and 4 CaKoV strains from Thailand were successfully identified. …

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Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit Jan 2023

Innovation Of Alpha-Mangostin Encapsulated With Nanostructured-Lipid Carrier As Non-Surgical Castration In Cats And Survey Attitude Towards Surgical Sterilization In Thailand, Shanaporn Leelakajornkit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Overpopulation of companion animals, particularly dogs and cats, is a global concern, necessitating effective population control policies. This research investigates the attitudes of veterinarians and pet owners in Thailand regarding dog and cat surgical sterilization and the use of progestins as non-surgical contraception. The study also assesses the effects of intratesticular administration of alpha-mangostin encapsulated in nanostructured lipid carriers (AM-NLC) in male cats, and adverse effects. Furthermore, a novel formulation of AM-NLC for intratesticular administration is also developed. Surveys reveal that decisions on sterilization are influenced by species, sex, COVID-19 impacts, and demographic factors. And anesthesia risks remain a primary …

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Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat Jan 2023

Developmental Model Of Endometrial Cell Culture For The Study Of Equine Endometrosis, Sawita Santiviparat

Chulalongkorn University Theses and Dissertations (Chula ETD)

The importance and rationale of this thesis were to address the critical knowledge gap in equine endometrosis by developing a comprehensive 3-dimensional (3D) in vitro model of equine endometrial tissue using advanced technology like 3D bioprinting. Study 1 aimed to develop an in vitro model for studying equine endometrial function by isolating and characterizing equine endometrial epithelial cells (eECs) and endometrial mesenchymal stem cells (eMSCs) using a modified tissue explantation outgrowth technique. Both cell types were successfully cultured in specific medium conditions, maintaining their characteristic features. Notably, eECs retained their function in fetal bovine serum (FBS) and KnockOut™ serum replacement …

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Value-Added Natural Fiber As A Biocompatible Substrate For Chemical Sensor Applications, Wisarttra Phamonpon Jan 2023

Value-Added Natural Fiber As A Biocompatible Substrate For Chemical Sensor Applications, Wisarttra Phamonpon

Chulalongkorn University Theses and Dissertations (Chula ETD)

This dissertation focuses on the improvement and value added of natural fiber extracted from the UHT milk carton waste, and raw silk cocoon to fabricate the biocompatible substrate for chemical sensors, principally in colorimetric and electrochemical sensors, which is allocated in 3 main parts. Initially, the background studying of the surface modification and the function of natural fiber based chemical sensor for food quality control and clinical diagnosis will be explained in a review article. Subsequently, the development of recycled cellulose from UHT milk carton waste as a novel substrate of dual lactate sensor for milk spoilage indication will be …

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A Comprehensive Study Of African Horse Sickness Outbreak In The Northeastern Region Of Thailand: Outbreak Investigation, Genetic Characterization, And Longitudinal Serological Monitoring, Napawan Bunpapong Jan 2023

A Comprehensive Study Of African Horse Sickness Outbreak In The Northeastern Region Of Thailand: Outbreak Investigation, Genetic Characterization, And Longitudinal Serological Monitoring, Napawan Bunpapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

African horse sickness (AHS) results from infection with the African horse sickness virus (AHSV). The AHSV is a non-contagious and belongs to genus Orbivirus, family Reoviridae and primarily by Culicoides midges. AHS caused the devastating infection in equine population, including horses, ponies, European donkeys, and mules. AHS maintains its occurrence in southern African countries, it may possess threatening impact to the naïve regions such as Asia or Southeast Asia. The objectives of this study are 1) To investigate the emerging AHS outbreak in the Northeastern region of Thailand, 2) To characterize emerged AHSV in Thailand and 3) To monitor serological …

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Efficacy Of Nanogel From Grafted Hyaluronic Acid Saliva Substitutes In The Treatment Of Dry Mouth, Nongnapat Eakpunyakul Jan 2023

Efficacy Of Nanogel From Grafted Hyaluronic Acid Saliva Substitutes In The Treatment Of Dry Mouth, Nongnapat Eakpunyakul

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this study was to evaluate the efficacy of HA-pNIPAM spray or Hy-N spray in treating dry mouth and to evaluate patient satisfaction with Hy-N spray. This study was a randomized double-blind crossover study conducted on patients with dry mouth. They were given three saliva substitutes: Hy-N, placebo, and Biotène spray, which were randomly allocated in a different order. Each spray was used for 1 week, followed by a 3-day wash-out period. The instruction was to use the sprays four times a day. A total of twenty-nine patients completed the study. A significant increase in unstimulated whole saliva …

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Comparing Medium To Long-Term Esthetic, Clinical And Patient-Reported Outcomes Between Freehand And Computer-Assisted Dental Implant Placement, Sun Heng Jan 2023

Comparing Medium To Long-Term Esthetic, Clinical And Patient-Reported Outcomes Between Freehand And Computer-Assisted Dental Implant Placement, Sun Heng

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: The aim of the study was to compare medium to long-term clinical and patient reported outcomes between implants placed with Computer-assisted Implant Surgery (CAIS) and freehand protocols. Materials and Methods: Thirty dental implants in the anterior maxillary region with an average of 4 years after loading were assessed by means of Pink Esthetic Scores (PES), Marginal Bone Level (MBL), clinical and patient reported outcomes. Results: CAIS significantly outperformed freehand placement with regards to PES scores (p=0.011). Likewise, implants placed with CAIS showed significantly higher marginal bone levels (p<0.001). Bleeding on Probing, Probing depth and prevalence of mucositis did not differ between the groups, while no peri-implantitis was diagnosed. Conclusions: The use of CAIS lead to superior outcomes in terms of esthetics and marginal bone levels for implants placed in the aesthetic zone as observed in medium to long-term follow-up. No difference was however observed with regards to peri-implant mucosa inflammation.

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Analysis Of Gene Expression And Histology Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 In Rat Femur, Duangporn Yongyosrungrueng Jan 2023

Analysis Of Gene Expression And Histology Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 In Rat Femur, Duangporn Yongyosrungrueng

Chulalongkorn University Theses and Dissertations (Chula ETD)

The quality of bone healing is an important factor in dental implant treatment. Currently, there is only limited research on using mRNA encoding BMP-2 to improve the quality and quantity of bone. This study aimed to evaluate the effectiveness of bone healing in rat femur defect after delivery of dPBS, rhBMP-2 and mRNA-encoding BMP-2 (BMP-2 mRNA-LNP). Histology approach was used to measure the quantity of newly formed bone. RT-qPCR was used to study gene expression levels of BMP-2, OCN, BSP and TNF-α. Subsequently, RNA-Seq analysis was performed at six weeks post-operation. Histological study revealed that BMP-2 mRNA-LNP group had significantly …

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Micro-Computed Tomography And Mechanical Test Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 With Implant Placement In Rat Femur, Kanoksiri Jriyasetapong Jan 2023

Micro-Computed Tomography And Mechanical Test Of Bone Regeneration Using Mrna Encoding Bone Morphogenetic Protein-2 With Implant Placement In Rat Femur, Kanoksiri Jriyasetapong

Chulalongkorn University Theses and Dissertations (Chula ETD)

This research evaluates the effectiveness of a modified mRNA encoding bone morphogenetic protein-2 (m1Ψ-BMP-2 mRNA) with N1-methylpseudouridine modifications on bone regeneration and integration after implantation into rat femur defects. In the study, twelve femurs from six Sprague-Dawley rats underwent titanium wire insertation. These femur defects were then filled with either 5 μg of m1Ψ-BMP-2 mRNA, 15 μg of m1Ψ-BMP-2 mRNA, or Dulbecco's phosphate-buffered saline (dPBS) in a randomized manner (N=4 each group) Six weeks post-implantation, the rats were euthanized for evaluation using micro-computed tomography and a mechanical push-out test. The Micro-CT analysis focused on several parameters including total bone volume, …

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Gene Expression Of Soft Tissue Covering Bone Grafted With Xenograft In Human, Atithan Termworasin Jan 2023

Gene Expression Of Soft Tissue Covering Bone Grafted With Xenograft In Human, Atithan Termworasin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Soft tissue is one of many factors that influence the esthetic success of dental implants. This study explores soft tissue reaction between soft tissue covering bone grafted with xenograft and soft tissue in normal healing by analyzing the levels of inflammatory cytokine including TNF-α, IL-6, IL-8 and NF-kB1. Soft tissues are collected after 4 to 6 months of healing and analyzed by reverse transcription quantitative polymerase chain reaction (RT-qPCR). The result shows that the relative gene expression of TNF-α, IL-6, IL-8, and NF-kB1 are not significantly different in soft tissue covering xenograft compared to the ones in soft tissue covering …

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ปัจจัยเสี่ยงของการเกิดโรคข้ออักเสบติดเชื้อซัลโมเนลลาเมื่อเปรียบเทียบกับเชื้อแบคทีเรียอื่น: การศึกษาแบบเก็บข้อมูลย้อนหลัง, กรัณย์ ประสพสันติ Jan 2023

ปัจจัยเสี่ยงของการเกิดโรคข้ออักเสบติดเชื้อซัลโมเนลลาเมื่อเปรียบเทียบกับเชื้อแบคทีเรียอื่น: การศึกษาแบบเก็บข้อมูลย้อนหลัง, กรัณย์ ประสพสันติ

Chulalongkorn University Theses and Dissertations (Chula ETD)

ที่มาและปัญหาของงานวิจัย โรคข้ออักเสบติดเชื้อซัลโมเนลลาพบได้บ่อยในประชากรที่มีความผิดปกติของระบบภูมิคุ้มกัน การศึกษานี้เป็นการศึกษาแรกที่เปรียบเทียบปัจจัยเสี่ยง อาการและอาการแสดง ผลตรวจทางห้องปฏิบัติการ และแนวทางการรักษาโรคข้ออักเสบติดเชื้อซัลโมเนลลาเมื่อเปรียบเทียบกับข้ออักเสบติดเชื้อแบคทีเรียอื่น ระเบียบวิธีการวิจัย ศึกษาแบบเก็บข้อมูลย้อนหลังในผู้ป่วยโรคข้ออักเสบติดเชื้อแบคทีเรียในโรงพยาบาลจุฬาลงกรณ์ตั้งแต่ 1 มกราคม พ.ศ.2556 ถึง 31 ธันวาคม พ.ศ.2565 แบ่งเป็นกลุ่มข้ออักเสบติดเชื้อซัลโมเนลลา 30 ราย และกลุ่มข้ออักเสบติดเชื้อแบคทีเรียอื่น 120 ราย เพื่อเปรียบเทียบปัจจัยเสี่ยงและข้อมูลอื่น ๆ ข้างต้นระหว่างทั้งสองกลุ่ม ผลการศึกษา ผู้ป่วยโรคข้ออักเสบติดเชื้อซัลโมเนลลามีอายุที่น้อยกว่า (ค่ามัธยฐานคือ 54 ปีและ 63 ปีตามลำดับ; p<0.001) การติดเชื้อซัลโมเนลลา พบมากที่สุดที่ข้อสะโพก ร้อยละ 36.67 แตกต่างจากเชื้อแบคทีเรียอื่นซึ่งพบเพียงร้อยละ 7.50 (p<0.001) สรุป โรคข้ออักเสบติดเชื้อซัลโมเนลลาเมื่อเทียบกับเชื้อแบคทีเรียอื่นมีปัจจัยเสี่ยงคืออายุน้อยและการใช้ยาคอติโคสเตียรอยด์มีระยะเวลาก่อนมาโรงพยาบาลที่นานกว่าและข้ออักเสบที่พบบ่อยคือข้อสะโพก

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Molecular Survey Of Enterocytozoon Bieneusi In Bat Feces Collected From Various Areas Of Thailand, Chatuthanai Savigamin Jan 2023

Molecular Survey Of Enterocytozoon Bieneusi In Bat Feces Collected From Various Areas Of Thailand, Chatuthanai Savigamin

Chulalongkorn University Theses and Dissertations (Chula ETD)

Enterocytozoon bieneusi is a microsporidian pathogen that can cause gastrointestinal diseases such as chronic diarrhea, abdominal pain, and nausea in humans, particularly in immunocompromised individuals. This pathogen has been found in the feces of various animals, from wildlife to domestic animals, but there have been no reports of its detection in bats in Thailand. Given that bat feces are widely used as fertilizers in agriculture and that bat-infested caves are tourist destinations where people may encounter bat feces, there is a need to study the presence of Enterocytozoon bieneusi in bat feces in these areas. In this study, 16 out …

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Hospital-Prepared: Autologous Platelet Rich Fibrin (Prf) Membrane For Corneal Epithelial Defect, Sarisa Tangkhunchaisaeng Jan 2023

Hospital-Prepared: Autologous Platelet Rich Fibrin (Prf) Membrane For Corneal Epithelial Defect, Sarisa Tangkhunchaisaeng

Chulalongkorn University Theses and Dissertations (Chula ETD)

An autologous platelet rich fibrin (PRF) membrane, a new generation of platelet concentrate known to have an effect on enhancing corneal epithelial wound healing by supersaturating the wound with epitheliotropic growth factors and being a scaffold for cellular proliferation and migration. However, there is no standard protocol for ophthalmologic use. This study aims to compare the characteristics of hospital prepared PRF membrane in four different protocols. We propose the super low centrifugation technic to yield the higher platelet cells and epitheliotropic growth factors. Blood samples were collected in 3 healthy volunteers with 4 different centrifugation protocols (100 g/5min, 200g force/5 …

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Predictive Factors For The Outcome Of Lumbar Epidural Steroid Injection In Low Back Pain: A Prospective Analytic Study, Pornpan Chalermkitpanit Jan 2023

Predictive Factors For The Outcome Of Lumbar Epidural Steroid Injection In Low Back Pain: A Prospective Analytic Study, Pornpan Chalermkitpanit

Chulalongkorn University Theses and Dissertations (Chula ETD)

Background: This prospective analytic study aimed to investigate predictive factors for favorable outcomes in patients with lumbosacral radicular pain following epidural steroid injection (ESI). Methods: 120 patients diagnosed with lumbosacral radiculopathy underwent blood collection at two-time points (before and after ESI). At the 2-week follow-up, a favorable response to ESI was defined as a reduction in pain of at least 50.0%. Predictive factors such as pain manifestation, magnetic resonance imaging (MRI), and inflammatory cytokines were investigated. Results: A favorable response was observed in 60.8% of patients. The mean differences in serum inflammatory cytokines post-TFESI showed no significant variation between the …

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ผลของโปรแกรมการจัดการภาวะฟอสเฟตในเลือดสูงตามแนวคิดทฤษฎีการเปลี่ยนแปลงพฤติกรรมและการสัมภาษณ์เพื่อเสริมสร้างแรงจูงใจผ่านทางเทเลเฮลท์ ในผู้ป่วยที่ได้รับการบำบัดทดแทนไตโดยการฟอกเลือดด้วยเครื่องไตเทียม ในสถานการณ์การระบาดของเชื้อไวรัสโคโรนา 2019, อารมย์ ทองสันติ Jan 2023

ผลของโปรแกรมการจัดการภาวะฟอสเฟตในเลือดสูงตามแนวคิดทฤษฎีการเปลี่ยนแปลงพฤติกรรมและการสัมภาษณ์เพื่อเสริมสร้างแรงจูงใจผ่านทางเทเลเฮลท์ ในผู้ป่วยที่ได้รับการบำบัดทดแทนไตโดยการฟอกเลือดด้วยเครื่องไตเทียม ในสถานการณ์การระบาดของเชื้อไวรัสโคโรนา 2019, อารมย์ ทองสันติ

Chulalongkorn University Theses and Dissertations (Chula ETD)

การวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาผลของโปรแกรมการจัดการภาวะฟอสเฟตในเลือดสูงตามแนวคิดทฤษฎีการเปลี่ยนแปลงพฤติกรรมและการสัมภาษณ์เพื่อเสริมสร้างแรงจูงใจผ่านทางเทเลเฮลท์ในกลุ่มผู้ป่วยโรคไตเรื้อรังระยะสุดท้ายที่ได้รับการบำบัดทดแทนไตโดยการฟอกเลือดด้วยเครื่องไตเทียม (โปรแกรม TMT) ในสถานการณ์การระบาดของไวรัสโคโรนา 2019 การศึกษานี้เป็นการศึกษาเชิงทดลองแบบสุ่มและมีกลุ่มควบคุม มีผู้เข้าร่วมวิจัยที่ตรงตามเกณฑ์ทั้งหมด 80 คน ได้รับการสุ่มเข้ากลุ่มทดลองและกลุ่มควบคุม กลุ่มละ 40 คน ด้วยวิธีสุ่มแบบชั้นภูมิร่วมกับการใช้บล็อก โดยกลุ่มทดลองได้รับโปรแกรม TMT เปรียบเทียบกับกลุ่มควบคุม ซึ่งได้รับการดูแลแบบปกติตามมาตรฐานการรักษาในศูนย์ฟอกเลือดด้วยเครื่องไตเทียม วิเคราะห์ข้อมูลโดยใช้สถิติ Linear regression เพื่อเปรียบเทียบค่าเฉลี่ยของตัวแปรและสถิติ Fisher’s exact test เพื่อเปรียบเทียบตัวแปรเชิงกลุ่มระหว่างกลุ่มทดลองและกลุ่มควบคุมที่ระดับความเชื่อมั่นร้อยละ 95 ใช้ระยะเวลาในการศึกษา 24 สัปดาห์ ผลการศึกษาจากการวิเคราะห์ข้อมูลแบบ Intention to treat พบว่าระดับฟอสเฟตในเลือดของกลุ่มทดลองลดลงมากกว่ากลุ่มควบคุมอย่างมีนัยสำคัญทางสถิติ (ผลต่างค่าเฉลี่ย = -1.03, 95% CI = -1.77, -0.29) พฤติกรรมการบริโภคอาหารที่มีส่วนประกอบของฟอสฟอรัสในกลุ่มทดลองดีกว่ากลุ่มควบคุม (ผลต่างค่าเฉลี่ย = 37.76, 95% CI = 23.54, 51.98) ระดับความพร้อมในการเปลี่ยนแปลงพฤติกรรม ความเชื่อมั่นในการรับประทานยาจับฟอสเฟตและพฤติกรรมการรับประทานยาจับฟอสเฟตอย่างต่อเนื่องของกลุ่มทดลองดีกว่ากลุ่มควบคุมอย่างมีนัยสำคัญทางสถิติ p-value < 0.001, 0.025 และ 0.001 ตามลำดับ แม้ว่าความรู้เกี่ยวกับการป้องกันและจัดการภาวะฟอสเฟตในเลือดสูงของทั้งสองกลุ่มไม่แตกต่างกัน (ผลต่างค่าเฉลี่ย = 3.86, 95% CI = -0.57, 8.29) จากผลของการศึกษาแสดงให้เห็นว่าโปรแกรม TMT สามารถนำมาประยุกต์ใช้เพื่อช่วยลดระดับฟอสเฟตในเลือดให้กับผู้ป่วยได้

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The Optimization And Expression Of Human Serum Albumin In Nicotiana Benthamiana, Ittichai Sujarittham Jan 2023

The Optimization And Expression Of Human Serum Albumin In Nicotiana Benthamiana, Ittichai Sujarittham

Chulalongkorn University Theses and Dissertations (Chula ETD)

Human serum albumin (HSA), primarily derived from human plasma, is limited by supply constraints and safety concerns due to potential contamination with blood-borne pathogens. Given the global shortage, hundreds of tons of HSA are required for clinical and cell culture applications. To address this, our study explored the production of recombinant HSA using Nicotiana benthamiana as an alternative production platform. The transient expression of recombinant HSA in plants was optimized by adjusting the harvesting days, plants aged and the OD600 of Agrobacterium. These several parameters can impact recombinant protein expression levels in plants. An Ni-NTA affinity chromatography method was used …

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Virtual Simulation Platform To Support Learning In Column Selection For Gas Chromatography, Lianrong Zhang Jan 2023

Virtual Simulation Platform To Support Learning In Column Selection For Gas Chromatography, Lianrong Zhang

Chulalongkorn University Theses and Dissertations (Chula ETD)

In this research, a virtual simulation platform was developed to support lecture-based teaching for gas chromatography (GC) coupled with a flame ionization (FID) detector. This platform utilizes a computerized three-dimensional (3D) model created using Blender, Unity, Visual Studio, and starter assets programs. It mimics laboratory and experimental learning to optimize GC conditions, including column types, column length, column diameter, gas flow rate, and sample injection for the separation of kerosene, derivatized milk, and perfume from sources such as aircraft, plants, and perfume samples. A spreadsheet-based simulation was used to simulate chromatograms of defined chemical compositions under various conditions. The retention …

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Design And Synthesis Of Quaternized Hyaluronic Acid-Quercetin-Carrageenan Nanocomplexes For Anticancer Drug Delivery, Ifra Aslam Jan 2023

Design And Synthesis Of Quaternized Hyaluronic Acid-Quercetin-Carrageenan Nanocomplexes For Anticancer Drug Delivery, Ifra Aslam

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objective of this research is to synthesize a polyelectrolyte nano-complex for anticancer drug delivery, utilizing natural biopolymers with enhanced therapeutic capabilities, biodegradability, and low toxicity. Traditional anticancer drugs are often hydrophobic and pose significant challenges in delivery, as well as causing toxicity to normal cells. In this study, Plai extract, known for its anticancer properties, was employed into a novel polyelectrolyte complex carrier composed of quaternized hyaluronic acid-quercetin (Quat-HA-QT) with carrageenan. The design involved: (i) utilizing hyaluronic acid (HA) for its CD-44 receptor affinity on tumor cells, (ii) modifying HA with Quat-188 to impart a positive charge and incorporating …

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Scaffolds From Natural Polymers And Soy Protein For Cultured Meat Production, Utumporn Butkatanyoo Jan 2023

Scaffolds From Natural Polymers And Soy Protein For Cultured Meat Production, Utumporn Butkatanyoo

Chulalongkorn University Theses and Dissertations (Chula ETD)

To overcome the problems of livestock farming, cultured meat technologies have been developed. Cultured meat is an alternative way to produce meat by culturing cells in vitro using tissue engineering technology. The construction of cultured meat requires a scaffold to support cell proliferation and differentiation. Herein this research, polysaccharides namely high acyl gellan gum (HG) and konjac (KJ) together with a plant-based protein, soy protein isolate (SP) were used to develop scaffolds for cultured meat production. The composite scaffolds were prepared by freeze-drying and followed by ionic crosslinking. The composition of HG, KJ, and SP had a strong influence on …

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Heavy Metal Distribution Pattern And Potential Ecological Risk In Roadside Soils As A Function Of Highway Traffic, Wassamon Thongaiod Jan 2023

Heavy Metal Distribution Pattern And Potential Ecological Risk In Roadside Soils As A Function Of Highway Traffic, Wassamon Thongaiod

Chulalongkorn University Theses and Dissertations (Chula ETD)

Roadside soil contamination by heavy metals due to traffic pollution presents a significant environmental concern. Assessing concentration levels and evaluating ecological risks are crucial steps toward understanding and mitigating this issue. This study focuses on soil properties and the presence of arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), lead (Pb), and zinc (Zn) along two high-traffic highways in Thailand. The empirical geological background was determined by sampling the parent materials. Out of 40 roadside soil surface samples collected, the average levels of Cu, Pb, and Zn surpass background concentrations. Significant differences in heavy metal concentrations between the two highways …

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Development Of Novel Detection Methods For Food Safety Using State-Of-The-Art Technology Platforms, Prae Noppakuadrittidej Jan 2023

Development Of Novel Detection Methods For Food Safety Using State-Of-The-Art Technology Platforms, Prae Noppakuadrittidej

Chulalongkorn University Theses and Dissertations (Chula ETD)

Foodborne illness is a significant global public health concern, impacting both the economy and human health. These illnesses are caused by bacteria, viruses, parasites, toxins, and chemicals. This research developed two technology platforms for Bacillus cereus and tropane alkaloid compounds detections. First, traditional detection methods for B. cereus have limitations in long analysis time, and dependence on analysis and interpretation by skilled personnel. Therefore, this study introduces a robust detection method using peptide nucleic acids (PNAs) bearing with a prolyl-2-aminocyclopentanecarboxylic acid (ACPC) backbone as probes in a bead array method. PNAs with groEL, motB, and 16S rRNA sequences were covalently …

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Synthesis Of Mixed Metal High Entropy Oxyhalide For Photocatalytic Degradation Of Tetracycline, Chakgrid Noomak Jan 2023

Synthesis Of Mixed Metal High Entropy Oxyhalide For Photocatalytic Degradation Of Tetracycline, Chakgrid Noomak

Chulalongkorn University Theses and Dissertations (Chula ETD)

High entropy materials (HEMs) are new types of materials that contain five or more elements. HEMs have been applied in various applications. It has been proven that HEMs with multielement can provide good structural stability. However, the application of HEMs in the field of photocatalytic has rarely been reported. Therefore, it is interesting to construct HEMs to be used as photocatalysts. In this work, we designed and fabricated a mixed metal high entropy halide (SrxBa1-xBiO2Br), a novel photocatalyst that contains five elements in the structure, by incorporating metals (Sr and Ba) into bismuth oxybromide material (BiOBr) to enhance their stability …

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