Digital Commons Network^™

Deciphering The Mechanism And Function Of Hsp100 Unfoldases From Protein Structure, Grace Lee, Rebecca S Kim, Sang Bum Lee, Sukyeong Lee, Francis T F Tsai

Faculty, Staff and Students Publications

Hsp100 chaperones, also known as Clp proteins, constitute a family of ring-forming ATPases that differ in 3D structure and cellular function from other stress-inducible molecular chaperones. While the vast majority of ATP-dependent molecular chaperones promote the folding of either the nascent chain or a newly imported polypeptide to reach its native conformation, Hsp100 chaperones harness metabolic energy to perform the reverse and facilitate the unfolding of a misfolded polypeptide or protein aggregate. It is now known that inside cells and organelles, different Hsp100 members are involved in rescuing stress-damaged proteins from a previously aggregated state or in recycling polypeptides marked …

Mitochondrial Autophagy In Ischemic Aged Livers, Jae-Sung Kim, William C Chapman, Yiing Lin

2020-Current year OA Pubs

Mitochondrial autophagy (mitophagy) is a central catabolic event for mitochondrial quality control. Defective or insufficient mitophagy, thus, can result in mitochondrial dysfunction, and ultimately cell death. There is a strong causal relationship between ischemia/reperfusion (I/R) injury and mitochondrial dysfunction following liver resection and transplantation. Compared to young patients, elderly patients poorly tolerate I/R injury. Accumulation of abnormal mitochondria after I/R is more prominent in aged livers than in young counterparts. This review highlights how altered autophagy is mechanistically involved in age-dependent hypersensitivity to reperfusion injury.

Detection Of Biomarkers For Filoviral Infection With A Silicon Photonic Resonator Platform, Krista Meserve, Abraham J Qavi, M Javad Aman, Hong Vu, Larry Zeitlin, John M Dye, Jeffrey W Froude, Daisy W Leung, Lan Yang, Frederick W Holtsberg, Gaya K Amarasinghe, Ryan C Bailey

2020-Current year OA Pubs

This protocol describes the use of silicon photonic microring resonator sensors for detection of Ebola virus (EBOV) and Sudan virus (SUDV) soluble glycoprotein (sGP). This protocol encompasses biosensor functionalization of silicon microring resonator chips, detection of protein biomarkers in sera, preparing calibration standards for analytical validation, and quantification of the results from these experiments. This protocol is readily adaptable toward other analytes, including cytokines, chemokines, nucleic acids, and viruses. For complete details on the use and execution of this protocol, please refer to Qavi et al. (2022).

An Fmri Protocol For Administering Liquid Incentives To Human Participants, Debbie M Yee, Jennifer L Crawford, Todd S Braver

2020-Current year OA Pubs

This protocol describes the materials and approaches for administering liquid incentives to human participants during fMRI scanning. We first describe preparation of the liquid solutions (e.g., neutral solution and saltwater) and liquid delivery setups. We then detail steps to connect the setups to the computer-controlled syringe pump in the MRI control room, followed by procedures for testing the syringe pump dispensing a liquid bolus during the task. Description of custom software and required adapters for implementing the liquid setup are included. For complete details on the use and execution of this protocol, please refer to Yee et al. (2021).

A Systematic Approach To Provide Feedback To Presenters At Virtual And Face-To-Face Professional Meetings, S Beth Bierer, Anna Cianciolo, Heeyoung Han, Janice Hanson

2020-Current year OA Pubs

INTRODUCTION: To promote their personal and professional growth, medical educators need practical, actionable feedback on their scholarship, as well as guidance for documenting their scholarship in educator portfolios. We offer a framework and resources to provide formative and summative feedback to faculty, administrators, and/or learners delivering an oral presentation at a face-to-face or virtual health professions education meeting.

METHODS: In 2014, the leadership of the Central Group on Educational Affairs (CGEA) meeting planning committee developed and piloted a process to provide individuals with formative and summative feedback on their oral CGEA research presentations at face-to-face meetings and create a transparent …

Mouse Kidney Nuclear Isolation And Library Preparation For Single-Cell Combinatorial Indexing Rna Sequencing, Haikuo Li, Benjamin D. Humphreys

2020-Current year OA Pubs

Single-cell combinatorial indexing RNA sequencing (sci-RNA-seq3) enables high-throughput single-nucleus transcriptomic profiling of multiple samples in one experiment. Here, we describe an optimized protocol of mouse kidney nuclei isolation and sci-RNA-seq3 library preparation. The use of a dounce tissue homogenizer enables nuclei extraction with high yield. Fixed nuclei are processed for sci-RNA-seq3, and self-loaded transposome Tn5 is used for tagmentation in library generation. The step-by-step protocol allows researchers to generate scalable single-cell transcriptomic data with common laboratory supplies at low cost. For complete details on the use and execution of this protocol, please refer to Li et al. (2022).

Sovereign Lands, Brent D. Chicken, Amanda J. Dick

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Wyoming, John R. Chadd

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

West Virginia, Andrew S. Graham

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Virginia, Zachary H. Barrett

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Utah, Alan M. White

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Texas, Melissa Munson

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Tennessee, Westley A. Ketron

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Pennsylvania, Nathaniel I. Holland, Jon C. Beckman, Benedict J. Kirchner

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Oklahoma, Matt Schlensker, Sandra Fraley

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Ohio, Tim Mckeen, Melissa Grimes

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

North Dakota, William J. Black

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

New York, Delainey Banks

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

New Mexico, Blake C. Jones

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Nevada, Kevin Hivick

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Nebraska, Sarah Trainer

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Montana, Amanda J. Dick

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Missouri, Douglas J. Crouse

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Michigan, M. Vafa Barissi, Delainey Banks

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Maryland, Tia L. Churchfield

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Louisiana, Andreah Riedel

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Kentucky, Gary Holland

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Kansas, Steven Rhodes, Sarah Trainer

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Illinois, Joseph Negaard

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.

Colorado, Diana S. Prulhiere, David R. Little

Oil and Gas, Natural Resources, and Energy Journal

No abstract provided.