Digital Commons Network^™

Investigating The Biochemical Properties Of A Novel Mutation, A194v, In Human Rad51, Briana Vollbeer

All Theses

DNA double-strand breaks (DSB) are one of the most serious DNA lesions because improper repair of a DSB can lead to loss of heterozygosity, aneuploidy, and cancer. One of the primary pathways to repair DSBs is homologous recombination (HR). HR resects the DNA around the DSB and then uses homologous DNA as a template to restore the broken sequence. RAD51 has a vital function in this pathway by forming a nucleoprotein filament on a resected end of the DSB. The nucleoprotein filament searches for homology within the homologous DNA. Once homology is located, strand invasion followed by strand exchange occurs. …

Exploring Visitor Perceptions Of Popular Culture Tourism In Sleepy Hollow, New York, Danielle Baker Kreh

All Theses

Popular culture tourism can present itself in many forms including travel based on popular literature, film, and television series. Popular culture tourism destinations exist from the assumption that tourists wish to visit destinations based on their popular culture connections. In fact, destinations may choose to emphasize their popular culture connections for the purpose of attracting more visitors. Such is the case with Sleepy Hollow, New York, which has many popular culture connections including the original short story “The Legend of Sleepy Hollow” by Washington Irving. Using the village of Sleepy Hollow as an example, the purpose of this study is …

Potassium Level Prediction Of Peach Tree Leaves Using A Hyperspectral Camera And Multivariate Analysis, Megan Io Ariadne Salimbangon Abenina

All Theses

The use of hyperspectral imaging is a valuable piece of technology used in precision agriculture. Hyperspectral imaging system could help farmers and researchers alike in analyzing tissue samples quicker compared to the current way of running a plant tissue analysis on the different nutrient levels that often takes time in generating results. The objective of this research was to measure the potassium level of peach trees using its leaves with a hyperspectral camera. The Senop HSC-2 was used to capture images of peach tree leaves during the fall of 2020 and 2021. The collected data were used to create a …

Using In Vitro, In Silico, And In-Classroom Techniques To Address The Gender Data Gap In Health Care, Kelsey Watts

All Dissertations

Cardiovascular diseases (CVDs) are the leading cause of death worldwide in males (XY) and females (XX). Prior to menopause, females have a relative protection against serious cardiac pathologies compared to age-matched males. This phenomenon is widely attributed to the ovarian hormone estrogen. Unfortunately, hormone replacement therapy to maintain estrogen levels in postmenopausal females has overall adverse effects, and it is not recommended for long-term use or as a preventative measure for eCVDs. A major driver of CVDs, specifically heart failure, is cardiac fibrosis: the continued buildup of scar tissue that reduces the heart’s ability to pump. There are currently no …

Isolation And Characterization Of Extracellular Vesicles From Various Biological Matrices Using Capillary-Channeled Polymer (C-Cp) Fiber Solid-Phase Extraction Spin-Down Tips, Kaylan Jackson

All Dissertations

A number of recent works have emphasized the need to isolate nanometer-scale analytes, like extracellular vesicles (EVs), from various biologically-relevant fluids. Exosomes are a subset of small EVs that range from 30-200 nm in diameter that serve as biomolecular snapshots of their cell of origin containing mother cell-specific DNA, miRNA, mRNA, and proteins. As critical components of intercellular communication, exosomes and other EVs play significant roles in many physiological and pathological processes. Diverse populations of these vesicles can be collected from biofluids, including blood, saliva, and urine, from cell culture conditioned media and primary cells, and even from plant …

An Intergenerational Study Of The Entrepreneurial Nature Of Agritourism Operators, Will Culler

All Dissertations

Economic and non-economic trends have left farm operators of all ages contemplating enterprise diversification strategies to create advantages and to ensure their farms' sustainability for future generations. One such strategy is agritourism, in which a visitor to a working farm or other agricultural setting interacts with the farm landscape or participates in an agricultural process for tourism or leisure purposes. This study aims to contribute to academics, researchers, extension educators, practitioners, and farm service providers who offer training and resources to better equip current and future agritourism operators. The study tested the general hypothesis that agritourism operators' entrepreneurial goals and …

Modeling Electrostatics In Molecular Biology And Its Relevance With Molecular Mechanisms Of Diseases, Mahesh Koirala

All Dissertations

Electrostatics plays an essential role in molecular biology. Modeling electrostatics in molecular biology is complicated due to the water phase, mobile ions, and irregularly shaped inhomogeneous biological macromolecules. This dissertation presents the popular DelPhi package that solves PBE and delivers the electrostatic potential distribution of biomolecules. We used the newly developed DelPhiForce steered Molecular Dynamics (DFMD) approach to model the binding of barstar to barnase and demonstrated that the first-principles method could also model the binding. This dissertation also reflects the use of existing computational approaches to model the effects of Single Amino Acid Variations (SAVs) to reveal molecular mechanisms …

Development Of Plasmonic And X-Ray Luminescence Nanoparticles For Bioimaging And Sensing Applications, Meenakshi Ranasinghe

All Dissertations

This dissertation discusses the development of plasmonic and X-ray luminescence nanoparticles (~100 nm) to use in bioimaging and sensing applications. The nanoparticles have interesting optical properties compared to their atomic levels and bulk materials. The optical properties of nanomaterials can be controlled by changing size, shape, crystal structure, etc. Also, they have a large surface area that can be functionalized with biomolecules. Therefore, the optical properties and biofunctionalized nanomaterials are useful in biomedical applications such as targeted drug delivery, bioimaging, and sensing. The overall theme is to use nanoparticles with interesting optical properties compared to their atomic levels and bulk …

Reading Toward Breath: A Poetic Ecology Of Creative Reading, Jessica Schad Manuel

All Dissertations

Reading is an act of perception that begins in wonder and leads to wisdom. It is not a response to writing but a response-ability we have to interact with the world around us and the phenomena before us. Reading with the body leads to wisdom, and when we participate in our existence by being in the world, we are reading. So what is reading? Reading is making. We make connections, and we form relationships. The act of reading is more than a cognitive process and even surpasses the phenomenological encounter.

My research describes the reader’s relationship to the text as …

Cover Cropping To Improve The Sustainability Of Agronomic Production Systems, Ricardo St Aime

All Dissertations

The adoption rate of cover cropping among farmers in the Southeast is low due to the lack of knowledge regarding the suitable cover crop species and their management practices and the concerns of resource depletion for the subsequent cash crop. To generate information addressing the above knowledge gap, the present research was conducted with the following objectives: (1) to determine the optimal planting method for winter cover crops in the upstate of SC and whether it is influenced by the seeding rates (Project-1); (2) to determine suitable winter cover crops for the region based on biomass production, water use efficiency …

Heat Stress Response And Excystation In Entamoeba Histolytica, Irem Bastuzel

All Dissertations

Entamoeba histolytica is a water- and food-borne intestinal protozoan parasite that causes amoebiasis and liver abscess and is responsible for symptomatic disease in approximately 100 million people each year leading to ~ 100,000 deaths. The most common disease transmission follows the oral-fecal route, but it can also be transmitted by mechanical vectors such as animals carrying the amoeba from contaminated sources to water systems. In rare cases, disease transmission has been recorded in some patients in which men-to-men sexual practices were preferred.

The life cycle of E. histolytica starts through ingestion of infectious cysts, which are non-dividing, quadri-nucleated structures surrounded …

Integration Of Techno-Economic Analysis (Tea) And Life Cycle Assessment (Lca) For Sustainable Process Design, Roksana Mahmud

All Dissertations

For sustainable design, technology developers need to consider not only technical and economic aspects but also potential environmental impacts while developing new technologies. Techno-economic analysis (TEA) evaluates the technical performance and economic feasibility of a technology. Life cycle assessment (LCA) evaluates the potential environmental impacts associated with a product system throughout its life cycle from raw material extraction to disposal. Generally, TEA and LCA are performed separately for technology assessment. Understanding of the trade-off between economic and environmental performances is crucial for sustainable process design, which is not fully available if TEA and LCA is performed separately. In contrast, integration …

Understanding Vascular Pathologies In Diabetes: Optimizing Tissue Engineered Blood Vessels Resistant To Diabetic Complications, Bethany Lefeber

All Dissertations

Diabetes Mellitus, a major risk factor for cardiovascular disease, currently affects 463 million people, or 9.3% of the world’s population – with numbers estimated to reach 578 million people by 2030. Hyperglycemia, dyslipidemia, oxidative stress, and inflammation, major manifestations of diabetes, together, significantly increase the risk of vascular tissue damage. Vascular damage is primarily caused by the interaction of cells and extracellular matrix (ECM) components with the elevated levels of glucose and lipids circulating through the blood and in the ECM. ECM proteins, such as elastin and collagen, are subject to glycation and crosslinking via advanced glycation end products (AGE) …

Unlocking The Pediatric Transcriptome: A Combinatorial Approach For Total Rna Analysis, William Schleif

All Dissertations

The transcriptome is an oft-studied target in the pursuit to better understand the functional elements of the human genome as part of genomic research. The transcriptome possesses its own unique set of challenges that hamper utilization of RNA-based discoveries and translation into actionable findings. Scientific progress thus far has expanded annotated reference genes for protein-coding and non-coding (nc) regulatory transcripts, yet much of the variation from splicing events or retention of other regions remain an open challenge, especially in children. This dissertation explores the current state of diagnostic and methodological barriers in front of the full unlocking of the transcriptome, …

A Qualitative Exploration Of The Role Older Siblings Play In The College-Going Experiences Of Younger First-Generation College Students, Gretchen Waugaman

All Dissertations

This study looked at the roles that older siblings who have been through the college process themselves play in the college-going experiences of younger first-generation college students. A qualitative study was conducted with 10 FGC students at a large public four-year university in the Southeast of the United States of America who had older siblings who were either currently attending college or had recently graduated from a four-year college or university themselves. For this study, I answered the following research question:

What role do older FGC student siblings play in the college-going experiences of younger siblings?

Data was collected using …

Tessellated Structural-Architectural Systems: Experimental Testing And Interdisciplinary Student Projects, Grace F. Crocker

All Dissertations

This dissertation discusses a new structural system called a Tessellated Structural-Architectural (TeSA) system. These TeSA systems utilize architecturally appealing tessellations in load-bearing structures comprised of interlocking tiles in a pattern. This dissertation focuses on the design, construction, and structural behavior of these TeSA systems, as well as their value as an interdisciplinary learning tool.

This dissertation has 4 research objectives: 1) Demonstrate the fabrication and construction of a precast reinforced concrete (RC) TeSA shear wall system; 2) Measure the structural performance of the RC TeSA shear wall system; 3) Compare simplified shear and flexural analysis methods for RC TeSA shear …

Microbial, Chemical, And Functional Components In Kefir, Natto, And Feed Ingredients, Dana Mccurdy

All Dissertations

Kefir grains were purchased from online, home kitchen vendors in the United States (n = 22), inoculated into UHT milk, and incubated at 25°C for 22 hours. Results indicated lactobacilli, lactococci and yeast in all samples. Six out of 11 samples contained coliform, and all samples contained pseudomonal and staphylococcal species. Using targeted genome sequencing (16S) of kefir grain samples 1, 2, and 3 revealed that lactobacilli were the predominant genera of bacteria. ITS sequencing revealed different fungal species in each grain. Results indicated potential presence of menaquinone-4 and menaquinone-7 by UPLC-MS/MS analysis.

The fermentation of whole and ground soybeans, …

Mortality Among Patients With Covid-19 And Different Interstitial Lung Disease Subtypes: A Multicenter Cohort Study., Joy Zhao, Brandon Metra, Gautam George, Jesse Roman, Joseph Mallon, Baskaran Sundaram, Michael Li, Ross Summer

Division of Pulmonary, Allergy, and Critical Care Medicine Faculty Papers

No abstract provided.

Gender Neutral Parenting: Raising A Generation Outside The Gender Binary, Toni Noelle Martinez

Graduate Theses and Dissertations

In the United States, the social and cultural reality remains organized around the gender binary. The binary legitimizes itself on the widely held belief that gender is determined by biology and, therefore, is “natural.” By exploring and firmly placing gender as a cultural construct, this thesis looks at the possibilities of fracturing the binary. Borrowing from Stephan Hirschauer (1994) and Judith Butler’s (2004), this thesis theorizes what a gender neutral world could look like and examines how Gender Neutral Parents contribute toward a gender revolution. Gender Neutral Parents, a community that is mostly found online, represent a small group that …

Operationalizing The Gift Of Love (Gol) In Interpersonal Reconstructive Therapy (Irt): An Examination Of The Role Of Meaning Reconstruction In Therapeutic Change., Eliza Stucker-Rozovsky

Dissertations, 2020-current

Interpersonal Reconstructive Therapy (IRT) (Benjamin, 2003/2006) is an integrative, principles-based treatment approach and theory of psychopathology that conceptualizes the motivating factor that underlies personality psychopathology as the gift of love (GOL). That is, copy processes are learned and maintained to achieve psychic proximity to the important people and their internalized representations (IPIRs) that were the original teachers. Relinquishing this gift of love allows a person to re-orient their life towards uncovering and living by their own individually held meanings that will then define how they relate to themselves and their world. Spirituality and religion have historically helped individuals make meaning …

Dibutyl Amine Detection, Quantification, And Removal With Iron(Ii)Tetrasulfophthalocyanine (Fetspc), Kyle Bittner

Electronic Theses and Dissertations

Pesticide testing is of practical interest in various areas of today’s world due to their prevalence and toxicity. These areas include agriculture, environmental, chemical plants, and many others. Herbicides, like Roundup, contain secondary amines such as glyphosate. The ability to detect secondary amines could offer a method for glyphosate detection in agricultural and environmental samples. Secondary amines can interact with metal complexes through electron charge transfer. Such interactions can cause a change in the metal’s electron configuration and energy state and can be seen in the UV-Vis spectrum. Metal phthalocyanines (MPc’s) and other organometallic complexes have been of interest for …

Flora Of Doe Mountain Recreation Area, Johnson County, Tennessee, Benjamin Mccullough

Electronic Theses and Dissertations

A botanical inventory of Doe Mountain Recreation Area (DMRA) in northeastern Tennessee was conducted to help guide conservation-based management. A total of 484 species were found in DMRA, comprising 94 families, and 285 genera, 10 species listed in the state rare plant list, and 76 exotic species. Two species, Liatris virgata and Lycopodiella inundata, were new state records. Water in the Lycopodiella seep was an order of magnitude more acid than at other sites. An analysis of the wildland-urban interface showed that only 13% of the area was classified as uninhabited. The inventory-invasion index, introduced to quantify the relative …

Anti-Adipogenic Differentiation Effect Of Andrographolide On Human Bone Marrow Mesenchymal Stem Cells, Duangrat Tantikanlayaporn, Mintra Kaewkittikhun, Sirikul Manochantr, Pakpoom Kheolamai

Asian Medical Journal and Alternative Medicine

Introduction: Since Obesity has become a major public health concern, a strategy to prevent or reduce obesity is a priority. The inhibition of lipid droplet accumulation and adipogenesis process provides a target for treatment of obesity. Andrographolide (AP), the major bioactive phytoconstituent of Andrographis paniculate, processes diverse pharmacological properties including antioxidant and anti-inflammatory. However, the effects on adipogenic differentiation of hBM-MSCs were still unknown.

Objectives: We examined the effect of andrographolide (AP) on adipogenic differentiation of hBM-MSCs.

Methods: hBM-MSCs were cultured in adipogenic differentiation condition and treated withAndrographolide (1 - 10 µM) for 7 - 21 days. At the …

Inhibition Of Micrornas Promotes Osteogenic Differentiation Potential Of Mesenchymal Stem Cells Derived From Human Placenta, Ladda Meesuk, Jintamai Suwanprateeb, Pakpoom Kheolamai, Chairat Tantrawatpan, Duangrat Tantikanlayaporn, Sirikul Manochantr

Asian Medical Journal and Alternative Medicine

Introduction: Mesenchymal stem cells (MSCs) are multipotent stem cells that have a regenerative function. They can be classified into several cell types, including osteoblasts, adipocytes, and chondrocytes. MSCs can be isolated from the placenta with a non-invasive procedure. However, the osteogenic differentiation potential of MSCs derived from the placenta (PL-MSCs) is not very efficient. The investigation for improving the osteogenic differentiation potential of PL-MSCs is important for the application of PL-MSCs in the clinical field. MicroRNA, including miR-21, miR-27b, miR-29a, and let-7b, plays an important role as a post-transcriptional regulator in various processes. However, there is restricted data regarding the …

Financial Literacy And Consumer Well-Being: Examining The Role Of Gender Role Ideology, Lorena Garcia Ramon

Theses and Dissertations

Financial literacy, or the ability to process economic information and make informed decisions about financial planning, wealth accumulation, debt, and pensions, has been linked to beneficial financial behaviors that support financial well-being. Despite these benefits, consumers tend to underperform in financial literacy tests with women generally faring worse than men. This dissertation draws from gender roles literature and Expectancy-Value Theory to examine how, why, and when traditional attitudes regarding societal gender roles (i.e., gender role ideology) hinder women’s financial literacy and willingness to acquire financial knowledge. Moreover, it examines how financial literacy and willingness to …

Gene Therapy For Fibrodysplasia Ossificans Progressiva: Feasibility And Obstacles, Elisabeth M W Eekhoff, Ruben D De Ruiter, Bernard J Smilde, Ton Schoenmaker, Teun J De Vries, Coen Netelenbos, Edward C Hsiao, Christiaan Scott, Nobuhiko Haga, Zvi Grunwald, Carmen L De Cunto, Maja Di Rocco, Patricia L R Delai, Robert J Diecidue, Vrisha Madhuri, Tae-Joon Cho, Rolf Morhart, Clive S Friedman, Michael Zasloff, Gerard Pals, Jae-Hyuck Shim, Guangping Gao, Frederick Kaplan, Robert J Pignolo, Dimitra Micha

Department of Anesthesiology Faculty Papers

Fibrodysplasia ossificans progressiva (FOP) is a rare and devastating genetic disease, in which soft connective tissue is converted into heterotopic bone through an endochondral ossification process. Patients succumb early as they gradually become trapped in a second skeleton of heterotopic bone. Although the underlying genetic defect is long known, the inherent complexity of the disease has hindered the discovery of effective preventions and treatments. New developments in the gene therapy field have motivated its consideration as an attractive therapeutic option for FOP. However, the immune system's role in FOP activation and the as-yet unknown primary causative cell, are crucial issues …

Pharmacological Interventions For The Treatment And Control Of Shivering In Adult Patients Undergoing Elective Surgery Under Regional Anaesthesia: A Systematic Review And Meta-Analysis, Malika Hameed, Naureen Akber Ali, Khalid Ahsan, Mohsin Nazir

Department of Anaesthesia

Intraoperative shivering is quite common after regional anaesthesia, which not only increases the total body oxygen requirement but also causes discomfort to the patients. The aim of this systematic review is to determine the effectiveness of pharmacological agents administered intra-operatively for treating shivering in adult patients who are undergoing elective surgery under regional (i.e., central neuraxial) anaesthesia so that an optimal choice of an agent can be recommended for clinical application. A literature search was carried out using PubMed, Cochrane Library, CINAHL databases, and hand searches to identify relevant studies. After literature screening and information extraction, a systematic review was …

Immune Checkpoint Inhibitors In Luminal Gastrointestinal Malignancies: Going Beyond Msi-H/Dmmr, Tmb And Pd-L1, Daniel S. Lefler, Adam E. Snook, Babar Bashir

Department of Medical Oncology Faculty Papers

In luminal gastrointestinal tumors, immune checkpoint inhibitors (ICIs) targeting PD-1, PD-L1 and CTLA-4 have been investigated in multiple settings. The indications for these drugs are primarily dependent on specific biomarkers that imply immunogenicity: overexpression of PD-L1, tumor mutational burden, loss of mismatch repair proteins (dMMR) and/or high microsatellite instability status. Although these markers can be both predictive and prognostic, there is variability in how they are measured and used to guide therapies. Moreover, the use of ICIs can be further refined with a better understanding of the tumor microenvironment and interactions with other available therapies. The purpose of this review …

Infection And Transmission Determinants Of Flea-Borne Rickettsioses, Hanna J. Laukaitis

Graduate Theses and Dissertations (2019 - present)

The genus Rickettsia is comprised of Gram-negative, obligate intracellular bacteria that are spread by hematophagous arthropods. Elucidating the factors conferring rickettsial virulence has perplexed investigators for decades, complicated by the lack of efficient genetic tools necessary to uncover rickettsial- and vector-specific factors contributing to persistence. The advent of transposon mutagenesis has enabled the field to make vast developments in uncovering novel rickettsial mechanisms utilized in various host backgrounds. Thus, the aim of this study was to generate Rickettsia felis transposon mutants and characterize novel phenotypes associated with genetic disruption in an arthropod background. Distribution of rickettsiae is reliant on the …

Genomic Analysis And Characterization Of Surface Properties Of Naphthalene Degrading Acinetobacter Isolates, Gunn Emilie Berge

Graduate Theses and Dissertations (2019 - present)

No abstract provided.