Dysregulation Of Mir-10a Promotes Cancer Features In Cholangiocarcinoma, Matthieu Spriet

Theses & Dissertations

Cholangiocarcinoma is a primary liver cancer of the bile duct epithelium that exhibits microRNA-mediated control of tumor cell signaling. Strides toward new treatment rest on a better defining of cholangiocarcinoma tumor biology including the RNA-based layer of regulation. Additionally, there is a gap in knowledge on microRNA expression in human tissue. While there is RNA-seq data of microRNA expression in tissue, it does not differentiate between cell types, thus leaving unanswered questions about cell specific microRNA biology and expression.

Here, we identify miR-10a as an oncogenic microRNA acting through MAPK signaling. Using cholangiocarcinoma cell lines, we determined miR-10a is an …

Neuropilin-2 In Advanced Prostate Cancer And Its Targeting With Novel Small Molecule Inhibitors, Ridwan Islam

Theses & Dissertations

Despite the development of new therapies, clinical management of advanced metastatic castrationresistant prostate cancer (mCRPC) still remains challenging owing to the emergence of therapy resistance. Resistance to the therapies is driven by both AR and non-AR pathways. Non-AR pathway is characterized by a lineage switch that leads to the development of neuroendocrine-like (NE-like) prostate cancer (PCa), which has a fulminant clinical course due to the lack of effective treatment strategy. Therefore, novel molecular targets need to be identified to develop effective therapeutic regimen to improve the outcomes in advanced PCa patients including NE-like PCa. Recent investigation on advanced PCa patients …

Investigating The Role Of Sphingosine Kinases During Alphavirus Infection, Opeoluwa Oyewole

Theses & Dissertations

Several alphaviruses, critical human pathogens, have re-emerged in recent years. Alphaviruses are found on all continents and infect both invertebrates and vertebrates, including some aquatic animals. They are classified as arboviruses because hematophagous arthropods transmit them. Based on the symptoms they cause, alphaviruses are classified as either arthritogenic or encephalitic.

Rising global travel has led to outbreaks of infections in new regions, increasing the geographical ranges of alphaviruses and causing new infections in susceptible hosts. Currently, there are no approved prophylactic or therapeutic measures to prevent or treat alphavirus infections; prevention measures have focused on vector control. These measures are …

A Systematic Literature Review Of The Prevalence, Distribution, Exposure, And Human Health Risks Of Tire Microplastics And The Contribution Of Their Physicochemically Diverse Properties, Luke Glastad

Theses & Dissertations

The history of synthetic plastics dates back to their first uses in 1907, continues with their expansion through the middle of the century with a diverse array of new polymers, and are so omnipresent today that many label this age of Earth the Plasticene Era. Tire microplastics (TMPs) have been increasingly identified as one of the environment's most bountiful types of microplastics (MPs). TMPs mainly enter the environment as tire-wear particles (TWPs) and recycled tire crumb (RTC). TWPs, in particular, have a high degree of variability, which impacts their distribution, degradation, and risk for adverse human health effects. This review …

Documenting Difficult Cases: A Mixed Method Analysis, Thomas Daniel Knight

History Faculty Publications

This Special Issue of Genealogy examines the use of evidence, documentation, and methodology in family history and genealogical studies, and welcomes case studies that examine how to document individuals and relationships. A critical component of scholarly research focusing on the study of particular individuals or groups entails correctly identifying those individuals Historians, genealogists, historical demographers, and scholars in other disciplines sometimes undertake this sort of analysis. Often, research is uncomplicated if the research subject remained in a particular geographical area, or left a clear evidentiary trail, but what happens when historical documents do not clearly identify the research subject? Utilizing …

Warming Overcomes Dispersal-Limitation To Promote Non-Native Expansion In Lake Baikal, Larry L. Bowman Jr., Daniel J. Wieczynski, Lev Y. Yampolsky, David M. Post

ETSU Faculty Works

Non-native species and climate change pose serious threats to global biodiversity. However, the roles of climate, dispersal, and competition are difficult to disentangle in heterogeneous landscapes. We combine empirical data and theory to examine how these forces influence the spread of non-native species in Lake Baikal. We analyze the potential for Daphnia longispina to establish in Lake Baikal, potentially threatening an endemic, cryophillic copepod Epischurella baikalensis. We collected field samples to establish current community composition and compared them to model predictions informed by flow rates, present-day temperatures, and temperature projections. Our data and model agree that expansion is currently …

Does Shrub Encroachment Reduce Foraging Grass Abundance Through Plant-Plant Competition In Lesotho Mountain Rangelands?, Meredith Root-Bernstein, Colin Hoag

Anthropology: Faculty Publications

Shrub encroachment is understood to be an important problem facing rangeland ecosystems globally. The phenomenon is still poorly understood both in regard to its impacts (e.g., on diversity, productivity, and soil properties) and its causes. We study the impacts and causes of dwarf shrub encroachment in the highlands of Lesotho. There, shrubs have been described as indicators of generalized land degradation and soil erosion. Surprisingly, our findings show that grass abundance is not reduced by shrub abundance, but that forb abundance does decrease with shrub abundance. We suggest that not enough research has been done to examine the role of …

Deep Learning For Medical Imaging In A Biased Environment, Usman Mahmood

Theses

Deep learning (DL) based applications have successfully solved numerous problems in machine perception. In radiology, DL-based image analysis systems are rapidly evolving and show progress in guiding treatment decisions, diagnosing, localizing disease on medical images, and improving radiologists' workflow. However, many DL-based radiological systems fail to generalize when deployed in new hospital settings, and the causes of these failures are not always clear. Although significant effort continues to be invested in applying DL algorithms to radiological data, many open questions and issues that arise from incomplete datasets remain. To bridge the gap, we first review the current state of artificial …

Toddler Characteristics And Attention, Lani A. Taylor

Research Psychology Theses

Abstract

Individual differences have become increasingly relevant when addressing variability across child development. Literature bears evidence that these differences may impact a toddler’s ability to respond to Joint Attention (JA). JA refers to a communicative gaze and pointing between a child and another individual. JA has been recognized as being a critical milestone, to the formation and cultivation of shared awareness and attention of an event or object. JA is a skill that is critical to the development of future language acquisition (Vaughan Van Hecke et al., 2007). The aim of this study is to measure whether child characteristics, such …

Nonordered Dendritic Mesoporous Silica Nanoparticles As Promising Platforms For Advanced Methods Of Diagnosis And Therapies, S. Malekmohammadi, Riaz Ur Rehman Mohammed, H. Samadian, A. Zarebkohan, A. García-Fernández, G. R. Kokil, F. Sharifi, J. Esmaeili, M. Bhia, M. Razavi, M. Bodaghi, T. Kumeria, R. Martínez-Máñez

Department of Chemical and Biomolecular Engineering: Faculty Publications

Dendritic mesoporous silica nanoparticles (DMSNs) are a new generation of porous materials that have gained great attention compared to other mesoporous silicas due to attractive properties, including straightforward synthesis methods, modular surface chemistry, high surface area, tunable pore size, chemical inertness, particle size distribution, excellent biocompatibility, biodegradability, and high pore volume compared with conventional mesoporous materials. The last years have witnessed a blooming growth of the extensive utilization of DMSNs as an efficient platform in a broad spectrum of biomedical and industrial applications, such as catalysis, energy harvesting, biosensing, drug/gene delivery, imaging, theranostics, and tissue engineering. DMSNs are considered great …

The Child Care Crisis: Through The Social Ecological Lens, Jocelyne Saldana

Master's Projects and Capstones

Abstract

Objective - Working mothers face mental strain when returning to work due to the challenges of the current child care system. It is unclear how the instability of the child care sector impacts the mental health of working mothers.

Methods - The systematic review of literature was conducted to identify existing evidence to determine the influence access to quality, dependable, and affordable child care has on maternal mental health outcomes in working mothers. Multiple databases were used including PubMed and Scopus.

Results - Subsidized programs help reduce the cost of child care, however, income eligibility criteria makes it difficult …

Applications Of Population Pharmacokinetic Modeling In People Living With Hiv, Michelle M. Pham

Theses & Dissertations

Population pharmacokinetic modeling has become an important tool for the individualization of medicine in HIV care and clinical pharmacology in general. This dissertation explores many applications of population pharmacokinetic modeling to help identify drug exposure, drug-drug interactions, and sources of variability in populations living with HIV. Comprehensive HIV care involves simultaneous treatment of the disease and any co-infection and/or co-conditions.

The first objective was to identify potential drug-drug interactions affecting combination antiretroviral therapy (ART) consisting of efavirenz, tenofovir disoproxil fumarate and emtricitabine, from concomitant use of chemotherapy agents. Three models were developed with sparse sampling to characterize efavirenz, emtricitabine, and …

Current Challenges In Image-Guided Magnetic Hyperthermia Therapy For Liver Cancer, Anirudh Sharma, Erik Cressman, Anilchandra Attaluri, Dara L Kraitchman, Robert Ivkov

Faculty, Staff and Student Publications

For patients diagnosed with advanced and unresectable hepatocellular carcinoma (HCC), liver transplantation remains the best option to extend life. Challenges with organ supply often preclude liver transplantation, making palliative non-surgical options the default front-line treatments for many patients. Even with imaging guidance, success following treatment remains inconsistent and below expectations, so new approaches are needed. Imaging-guided thermal therapy interventions have emerged as attractive procedures that offer individualized tumor targeting with the potential for the selective targeting of tumor nodules without impairing liver function. Furthermore, imaging-guided thermal therapy with added standard-of-care chemotherapies targeted to the liver tumor can directly reduce the …

Ewing Sarcoma Transcriptome: Identification Of Foxn3 As A Novel Target Of Ews-Fli1, Natasha Kreiling

Theses & Dissertations

Ewing Sarcoma is an aggressive cancer characterized by a fusion gene formed from a chromosomal translocation between a RNA binding protein and a transcription factor. The most common fusion gene is EWS-FLI1 a potent oncogenic transcription factor responsible for initiating genome instability. EWS-FLI1 promotes tumor growth by dysregulating several genes and pathways. We have identified FOXN3, an important gene in cell cycle control and development, as being suppressed by EWS-FLI1. In the future, a better understanding of FOXN3 and its ability to act as a tumor suppressor in EWS will unravel novel mechanisms of EWS pathogenesis, potential biomarker and drug …

Youth Climate Change Activism: How Activism In Youth Can Be Encouraged To Help Combat Climate Change And Reduce Youth Health Impacts, Lorely M. Ruiz

Master's Projects and Capstones

While the effects of climate change are already affecting populations throughout the world, youth will have to deal with the full array of consequences due to the inaction of people in power and environmental regulation failures. Climate change is an issue that needs to be dealt with urgently due to the disproportionate burden it has on youth and future generations. A scoping literature review was conducted to analyze existing literature on climate change activism among youth throughout the globe. Research, practice, and policy recommendations were made to help address gaps in existing literature such as limited research on youth climate …

Visual Factors Associated With Physical Activity In Schoolchildren, Síofra Harrington, John Kearney Prof, Veronica O'Dwyer Dr

Articles

Clinical relevance Physical activity is an essential part of childhood physical and mental development. Recent research identified visual problems associated with a sedentary lifestyle in children in Ireland.

Background This study explored the association between visual function in children and their engagement with physical activities outside school.

Methods Participants were 1,626 schoolchildren (728 aged 6–7 years, 898 old 12–13 years) in randomly selected schools in Ireland. Before data collection, parents/legal guardians of participants completed a standardised questionnaire reporting physical activity as no activity (mostly on screens), light activity (occasional walking/cycling), moderate activity (/week engaged in sports), or regular activity ( …

Agrichemicals (Nitrate And Atrazine) In Drinking Water And Adverse Health Outcomes In Children In Nebraska, Balkissa S. Ouattara

Theses & Dissertations

Objectives: This research was conducted to (1) determine the concentrations of atrazine, and nitrate, in Nebraska watersheds and counties; (2) calculate the incidence of pediatric cancers and prevalence of birth defects in Nebraska counties and watersheds, respectively; (3) assess the relationship between the contaminant levels and the incidence rate of pediatric cancers and birth defects prevalence.

Methods: Pediatric cancers and birth defects data were obtained from the Nebraska Department of Health and Human Services. Water quality data were collected and retrieved from the Water Quality Portal and the Nebraska Groundwater Quality Clearinghouse. Geospatial and statistical analyses were conducted at the …

Scratching The Surface: An Autoethnography To Begin To Understand And Address Illness Experience In Lice Infestation, Jennifer B. Gray

The Qualitative Report

Though pediculosis, more commonly known as lice, is extremely common and has nothing to do with hygiene, misconceptions persist. Lice, constructed as a highly contagious illness, is more of a nuisance, with most contagion resulting from head-to-head contact, and 3% resulting from environmental causes; still, the condition tends to be associated with negative behavior like uncleanliness and neglect. There is very little study of it in the U.S. save for some “no nit policy” studies and almost none on the psychological or communicative impact on those affected. Through the analysis of pediculosis in my children, I detail an autoethnography of …

Interventions To Support Teen Mothers In California And Reduce The Disparities Related To The Effects Of Teen Pregnancy, Shanelle Christine Sanchez

Master's Projects and Capstones

The US teen birth rate has slowly been declining; however, teenage pregnancy is still prevalent. In 2018 the US teen birth rate was 17.4 births per 1,000 girls aged 15-19 years, totaling 179,871 births (National Vital Statistic Report). California’s most recent birth rate was 13.6 births per 1,000 girls aged 15-19 years in 2020 (CDC, 2020). Few studies focus on teen mothers’ health, life needs, and outcomes as a result of adolescent pregnancy and subsequently, adolescent parenting. Additionally, few studies have looked at the cultural differences and views of adolescent pregnancy and parenting within the Hispanic population, which are greatly …

Reframing Culturally Competent Care To Improve Mental Health Outcomes Among African Americans, Jaelen Kennedy-Valdez

Master's Projects and Capstones

African Americans are continuously disadvantaged when it comes to interacting with mental health services which has been evident in the utilization of services, treatment engagement, and quality of services compared to their White counterparts (Alang, 2019). Many researchers and providers have called for culturally competent care and tailored treatment for African Americans to address barriers to access and quality of health care (Alang, 2019). In this paper, a systematic review will be conducted to assess several models that have been integrated in the U.S. healthcare settings and services. In addition, we will define the components of culturally competent care and …

Latinx Women Fighting Stress With Exercise, Joanna Sanchez

Master's Projects and Capstones

Previous research had identified that routine physical activity improves mental and physical health outcomes. This extensive literature review will focus on Latinx women who engage in routine physical active activities and are able to decrease stress. The literature will mention various stressors that Latinx women encounter in their way of living that prevent them from participating in routine physical activities. Peer-reviewed articles were examined to determine if physical activity buffer stress among Latinx women. Various literature databases used to extract articles from 2013 to 2022. Recommendations to assist the targeted population will use the principles of the Health Belief Model, …

Size Structuring Of Myctophids In The Northern Gulf Of Mexico In The Years Following The Deepwater Horizon Oil Spill, Adam Warren

All HCAS Student Capstones, Theses, and Dissertations

Body size is one of the main determinants of marine ecosystem structure and is correlated with many behavioral processes such as diel vertical migration (DVM). Myctophidae, a highly abundant, speciose, and globally distributed fish family, perform diel vertical migrations between the epipelagic zone at night and the mesopelagic zone during the day with vertical distributions varying with ontogeny, and therefore body length. Understanding how DVM contributes to an ecosystem’s structure is important to understanding ecosystem functioning, especially in response to anthropogenic impacts such as the Deepwater Horizon oil spill. The aim of this study was to investigate changes in myctophid …

Taxonomic Resolution Assessment And Biodiversity Evaluations For Deep-Sea Fish Assemblages In A Pelagic Ecosystem Of The Northern Gulf Of Mexico, Krista N. Scheuerman

All HCAS Student Capstones, Theses, and Dissertations

Knowledge of community assemblages and biodiversity is important for monitoring health and resilience in an ecosystem. Taxonomic and functional biodiversity of mesopelagic (200- 1000 m) fishes is extremely rich in the Gulf of Mexico. The aim of this study was to compare calculations of biodiversity and community structures at varying taxonomic resolutions for deep pelagic fishes to inform future decisions about deep-sea ecosystem monitoring. This study analyzed biodiversity and assemblage structure patterns from a biological inventory of deep-sea fishes collected with a large mesh, commercial-sized, high-speed rope trawl in the Gulf of Mexico between June 21^st and July 14 …

Effects Of Net Type On The Quantification Of Pelagic Shrimp And Krill In The Gulf Of Mexico, Eric J. Lee

All HCAS Student Capstones, Theses, and Dissertations

Trawls are an effective and widely used method for collecting micronekton because they can sample large volumes of sparsely distributed organisms, as well as allow for direct species identification of collected samples. However, net sampling methods are known to be highly variable in terms of design and catchability, and comparisons of deep-sea trawl data from two different types of nets over the same spatial and temporal scale are relatively rare. The current study is unique because it provides such an analysis for micronektonic crustaceans in the Gulf of Mexico (GOM) by comparing the trawling efficacy of a smaller 10 m …

Foraging Habitat Selection Of Shrubland Bird Community In Tropical Dry Forest, Anant Deshwal, Steven L. Stephenson, Pooja Panwar, Brett A. Degregorio, Ragupathy Kannan, John D. Willson

Biological Sciences Faculty Publications and Presentations

Habitat loss due to increasing anthropogenic disturbance is the major driver for bird population declines across the globe. Within the Eastern Ghats of India, shrubland bird communities are threatened by shrinking of suitable habitats due to increased anthropogenic disturbance and climate change. The development of an effective habitat management strategy is hampered by the absence of data for this bird community. To address this knowledge gap, we examined foraging sites for 14 shrubland bird species, including three declining species, in three study areas representing the shrubland type of forest community in the Eastern Ghats. We recorded microhabitat features within an …

Making The Rounds: Exploring The Role Of Circulating Tumor Dna (Ctdna) In Non-Small Cell Lung Cancer, Misty Dawn Shields, Kevin Chen, Giselle Dutcher, Ishika Patel, Bruna Pellini

2020-Current year OA Pubs

Advancements in the clinical practice of non-small cell lung cancer (NSCLC) are shifting treatment paradigms towards increasingly personalized approaches. Liquid biopsies using various circulating analytes provide minimally invasive methods of sampling the molecular content within tumor cells. Plasma-derived circulating tumor DNA (ctDNA), the tumor-derived component of cell-free DNA (cfDNA), is the most extensively studied analyte and has a growing list of applications in the clinical management of NSCLC. As an alternative to tumor genotyping, the assessment of oncogenic driver alterations by ctDNA has become an accepted companion diagnostic via both single-gene polymerase chain reactions (PCR) and next-generation sequencing (NGS) for …

System Dynamics Modeling For Traumatic Brain Injury: Mini-Review Of Applications, Erin S. Kenzie, Elle L. Parks, Nancy Carney, Wayne Wakeland

Complex Systems Faculty Publications and Presentations

Traumatic brain injury (TBI) is a highly complex phenomenon involving a cascade of disruptions across biomechanical, neurochemical, neurological, cognitive, emotional, and social systems. Researchers and clinicians urgently need a rigorous conceptualization of brain injury that encompasses nonlinear and mutually causal relations among the factors involved, as well as sources of individual variation in recovery trajectories. System dynamics, an approach from systems science, has been used for decades in fields such as management and ecology to model nonlinear feedback dynamics in complex systems. In this mini-review, we summarize some recent uses of this approach to better understand acute injury mechanisms, recovery …

Improved Sars-Cov-2 Sequencing Surveillance Allows The Identification Of New Variants And Signatures In Infected Patients, Antonio Grimaldi, Francesco Panariello, Patrizia Annunziata, Teresa Giuliano, Michela Daniele, Biancamaria Pierri, Chiara Colantuono, Marcello Salvi, Valentina Bouché, Anna Manfredi, Maria Concetta Cuomo, Denise Di Concilio, Claudia Tiberio, Mariano Fiorenza, Giuseppe Portella, Ilaria Cimmino, Antonio Sorrentino, Giovanna Fusco, Maria Rosaria Granata, Pellegrino Cerino, Antonio Limone, Luigi Atripaldi, Andrea Ballabio, Davide Cacchiarelli

Duncan NRI Faculty and Staff Publications

Background: Genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the only approach to rapidly monitor and tackle emerging variants of concern (VOC) of the COVID-19 pandemic. Such scrutiny is crucial to limit the spread of VOC that might escape the immune protection conferred by vaccination strategies or previous virus exposure. It is also becoming clear now that efficient genomic surveillance would require monitoring of the host gene expression to identify prognostic biomarkers of treatment efficacy and disease progression. Here we propose an integrative workflow to both generate thousands of SARS-CoV-2 genome sequences per week and analyze host …

Plasmonic Fluor-Enhanced Antigen Arrays For High-Throughput, Serological Studies Of Sars-Cov-2, Abraham J Qavi, Chao Wu, Matthew Lloyd, Mohammad Mahabub-Uz Zaman, Jingyi Luan, Claire Ballman, Daisy W Leung, Scott L Crick, Christopher W Farnsworth, Gaya K Amarasinghe

2020-Current year OA Pubs

Serological testing for acute infection or prior exposure is critical for patient management and coordination of public health decisions during outbreaks. Current methods have several limitations, including variable performance, relatively low analytical and clinical sensitivity, and poor detection due to antigenic drift. Serological methods for SARS-CoV-2 detection for the ongoing COVID-19 pandemic suffer from several of these limitations and serves as a reminder of the critical need for new technologies. Here, we describe the use of ultrabright fluorescent reagents, Plasmonic Fluors, coupled with antigen arrays that address a subset of these limitations. We demonstrate its application using patient samples in …

Addressing Racial Disparities In Maternal Health: The Case For An Equity Birth Plan, Alice Trye, Damali Campbell Oparaji, Natalie Roche, Lisa Gittens Williams

Journal of Health Disparities Research and Practice

Birth equity can be defined as “The assurance of the conditions of optimal births for all people with a willingness to address racial and social inequities in a sustained effort.” (Birth Equity |California Maternal Quality Care Collaborative, n.d.). Complex explanations exist for adverse maternal outcomes, but unequal health care, socioeconomic and racial inequalities pose risks to women and their babies (“Unequal Treatment: Confronting Racial and Ethnic Disparities in Health Care,” 2003). The data is clear: Maternal death rates have increased and are 3 to 4 times higher for black women than white women (Zaharatos et al., 2018). These …