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2022

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Orthodontic Treatment To Improve Periodontal Defect In A Patient With Lower First Molar Extraction– A Case Report, Yu-Ling Cheng, Yuen-Yung Tsang, Chia-Yi Pan, Ming-Juean Su Aug 2022

Orthodontic Treatment To Improve Periodontal Defect In A Patient With Lower First Molar Extraction– A Case Report, Yu-Ling Cheng, Yuen-Yung Tsang, Chia-Yi Pan, Ming-Juean Su

Taiwanese Journal of Orthodontics

A 23 year-old male came with chief complaint of lower anterior crowding and protrusive lower lip. Dental examination revealed missing teeth 14, 24 and 34 and horizontally impacted 38 and 48. Poor prognosis of tooth 36 was found after evaluation. Anterior cross bite and open bite at his left side occlusion were also seen. In this case, removal of teeth 36 and 38 were selected instead of 44 extraction. Thus the timing and proper sequence of tooth extraction become critical. The biomechanics of tooth movement was designed to ensure that all teeth are in functional occlusion and the lower left …

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Relationship Between Orthodontic Treatment And Root Resorption – A Literature Review, Yu-Tnig Wu, Wen-Ken Tai, Hoi-Shing Luk, Chih-Chen Chou, Heng-Ming Mark Chang, Chia-Tze Kao Aug 2022

Relationship Between Orthodontic Treatment And Root Resorption – A Literature Review, Yu-Tnig Wu, Wen-Ken Tai, Hoi-Shing Luk, Chih-Chen Chou, Heng-Ming Mark Chang, Chia-Tze Kao

Taiwanese Journal of Orthodontics

Shorting of tooth or external root resorption that happen during or after completion of orthodontic treatment are troublesome. The root resorption could affect treatment outcome, unstable orthodontic tooth movement and even cause medical dispute. The etiologies of external root resorption could be systemic problems or local factors. The mechanism of external root resorption is still unclear and under investigation. This article reviewed the factors and possible causes on the orthodontic root resorption and discussed the possible role of light emitted diode (LED) biophotostimualtion effects to inhibit the root resorption. The information was provided for orthodontists in daily practice.

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The Prevalence And Patterns Of Tooth Agenesis Among Bilateral Cleft Lip And Palate Patients - A Retrospective Study, Li-Tyng Sun, Te-Ju Wu, Yu-Jen Chang, Jui-Pin Lai, Faye Huang, Wen-Ching Tsai, Tzu-Ying Chen, Kai-Shin Lee, Shiu-Shiung Lin Aug 2022

The Prevalence And Patterns Of Tooth Agenesis Among Bilateral Cleft Lip And Palate Patients - A Retrospective Study, Li-Tyng Sun, Te-Ju Wu, Yu-Jen Chang, Jui-Pin Lai, Faye Huang, Wen-Ching Tsai, Tzu-Ying Chen, Kai-Shin Lee, Shiu-Shiung Lin

Taiwanese Journal of Orthodontics

Objective: Tooth agenesis is quite common in cleft lip and palate patients. This study was aimed for retrospectively investigating the prevalence and patterns of tooth agenesis among bilateral cleft lip and cleft palate (CLCP) patients. Patients: The hospital data and x-ray films of bilateral CLCP patients no younger than 7 years old in the Craniofacial Center, Kaohsiung Chang Gung Memorial Hospital from June 1974 to September 2015 were selected. The enrolled list contained 188 patients (male: 124; female: 64). The mean age of our patient was 10.13 years (range: 7.0 ~ 23.6 years). Materials and methods: Patients’ dental records and …

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Treatment Of Severe Class Ii Division 1 Malocclusion With Maxillary First Molar Missing — Case Report, Ju-Chi Ho, Chien-Cheng Chen, Yi-Horng Chen Aug 2022

Treatment Of Severe Class Ii Division 1 Malocclusion With Maxillary First Molar Missing — Case Report, Ju-Chi Ho, Chien-Cheng Chen, Yi-Horng Chen

Taiwanese Journal of Orthodontics

This case report describes a 22-year-old female with skeletal Class II and dental Class II division 1 malocclusion. The chief complaint is her protrusive upper front teeth. Clinical examination revealed a convex facial profile, retrognathic mandible, proclined upper and lower incisors with 11mm overjet, missing of upper left first molar, mild crowding in the lower anterior region and V-shaped arch forms. After orthodontic analysis and communication with the patient, the treatment plan was decided to orthodontic camouflage treatment by extracting the upper first premolars, moving upper second and third molars mesially at left side and maintaining upper second premolar to …

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Literature Review: The Effect Of Timing Of Loading And Osseointegration On Stability And Success Rate Of Orthodontic Mini-Screws, Hsiu-Ching Ko, Pi-Huei Liu, Chih-Liang Ho, Wen-Pei Wong, Hsiang-Hua Lai, Min-Chih Hung Aug 2022

Literature Review: The Effect Of Timing Of Loading And Osseointegration On Stability And Success Rate Of Orthodontic Mini-Screws, Hsiu-Ching Ko, Pi-Huei Liu, Chih-Liang Ho, Wen-Pei Wong, Hsiang-Hua Lai, Min-Chih Hung

Taiwanese Journal of Orthodontics

With the advances of dental implants, temporary skeletal anchorage devices have now become established orthodontic anchorage aids and launched a new era for clinical orthodontic therapy. Nowadays, orthodontic miniscrews are commonly used in daily practice and demand great stability and high predictability for therapeutic needs. In recent years, numerous publications have investigated multiple factors affecting success rates of orthodontic mini-screws. The aims of this review were to investigate the relationships between timing of loading and implant stability, and the impact of osseointegration on orthodontic mini-screws.

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Literature Review: Corticotomy-Assisted Orthodontic Treatment, Lei Kang, Shou-Hsin Kuang, Tzu-Ying Wu Aug 2022

Literature Review: Corticotomy-Assisted Orthodontic Treatment, Lei Kang, Shou-Hsin Kuang, Tzu-Ying Wu

Taiwanese Journal of Orthodontics

Corticotomy-assisted orthodontics (CAOT) can shorten orthodontic treatment time by increasing regional acceleratory phenomenon. The periodontal wound healing caused by corticotomy are similar to the periodontal tissue reaction induced by orthodontic force; if the two phenomena could be accumulated and both enhancing bone remodeling, orthodontic tooth movement should be accelerated theoretically. There are many different kinds of procedures and strategies in the clinical application of corticotomy-assisted orthodontics, but when the cost and benefit ratio for the patients are thoroughly considered, using minimal invasive corticotomy to assist orthodontic treatment may be more practical. As for the clinical efficacy of minimal invasive corticotomy, …

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An Investigation On Mechanical Properties Of Esthetic Orthodontic Archwires, Po-Yu Yang, Tzu-Hsin Lee Aug 2022

An Investigation On Mechanical Properties Of Esthetic Orthodontic Archwires, Po-Yu Yang, Tzu-Hsin Lee

Taiwanese Journal of Orthodontics

The main purpose of this study was to investigate the mechanical properties of Ni-Ti coated orthodontic archwires after surface modification. Universal test machine was used to measure the tensile strength of four groups of coated Ni-Ti wires and uncoated control group. Immersion test was applied for 21 days to discover whether the coating would peel off under friction and brushing after immersion. Student’s t-test was used to explore any difference between three groups of coated wires and uncoated conventional wires in mechanical properties. The results showed no statistical difference exists between coated Ni-Ti wires and conventional uncoated wires regarding to …

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Profile Angle Defines Soft Tissue Chin Position In Adults, Hui-Ling Chen, Yueh-Tse Lee, Yu-Ching Wang Aug 2022

Profile Angle Defines Soft Tissue Chin Position In Adults, Hui-Ling Chen, Yueh-Tse Lee, Yu-Ching Wang

Taiwanese Journal of Orthodontics

Objectives: To identify the soft tissue chin position relative to the upper part of the face wherein the Frankfort horizontal (FH) plane intersects the nasal bridge and to define a specific measurement that might benefit different racial groups and their treatment goals. Materials and Methods: Forty-eight Taiwanese adults (23 males and 25 females), selected based on criteria consisting of proper occlusion, skeletal Class I pattern, lip competence in repose, and within the Esthetic (E) line confined standard, were subjected to standard soft tissue facial analyses. The results were used to compare with those of the White norm. Results: Analyses revealed …

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Literature Review: Low Level Laser Therapy In Orthodontics, Meng-Huan Tsai, Hsuan-Yu Shih, Hui-Chen Tsai, Hsuan-Yi Hsiao, Chung-Chen Jane Yao Aug 2022

Literature Review: Low Level Laser Therapy In Orthodontics, Meng-Huan Tsai, Hsuan-Yu Shih, Hui-Chen Tsai, Hsuan-Yi Hsiao, Chung-Chen Jane Yao

Taiwanese Journal of Orthodontics

Treatment duration is one of the major determinants for patients receiving orthodontic treatment. To avoid long treatment time, orthodontists have to design sound mechanics meeting the anchorage demands and delivering appropriate force system to the tooth and its supporting tissues. However, the more crucial factors related to the treatment efficiency may lie in the individual biological response which is still largely unknown, to the force system which orthodontists regularly delivered. Low level laser with specific power setting has been shown as a non-invasive adjunctive treatment without obvious side effect for facilitating orthodontic movement in some clinical studies. This article reviews …

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Optimizing Facial Esthetics In Ogs Patients, Meng-Huan Tsai, Shao-Chun Lu, Joung-Lin Liaw, Ming-Zen Su, Ya-Ting Wang, Hsin-Fu Frank Chang, Chung-Chen Jane Yao, Stella Ya-Hui Yang Aug 2022

Optimizing Facial Esthetics In Ogs Patients, Meng-Huan Tsai, Shao-Chun Lu, Joung-Lin Liaw, Ming-Zen Su, Ya-Ting Wang, Hsin-Fu Frank Chang, Chung-Chen Jane Yao, Stella Ya-Hui Yang

Taiwanese Journal of Orthodontics

At present, even if the orthognathic surgical procedure had been improved and the acceptance of surgery increased, the patients and dentists could not be satisfied with simply the recovery of masticatory function. Facial esthetics has become a marked issue. However, everything has its own limitation. There is no single treatment could fulfill all the facial esthetics requirements, not even the orthognathic surgery. If the orthognathic surgery could be combined with other cosmetic surgical procedure, the patients would be more likely to receive relatively compre-hensive treatment. First, this article reviewed the fundamental definition of facial esthetic in psychology. In addition, we …

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Correction Of Severe Class Ii Division 1 Malocclusion With Miniscrew Anchorage– A Case Report, Yuh-Jia Hsieh, Ellen Wen-Ching Ko, Yu-Fang Liao, Chiung-Shing Huang Aug 2022

Correction Of Severe Class Ii Division 1 Malocclusion With Miniscrew Anchorage– A Case Report, Yuh-Jia Hsieh, Ellen Wen-Ching Ko, Yu-Fang Liao, Chiung-Shing Huang

Taiwanese Journal of Orthodontics

A case report describes the camouflage treatment of an adult patient with skeletal Class II jaw base relationship and moderate crowding. A 28-year-old woman presented with chief complaints of protrusive lips and upper front teeth. The clinical examination showed a convex facial profile, retrognathic mandible, excessively proclined upper and lower incisors, large overbite and overjet and a Class II division 1 malocclusion. To correct the facial profile, lip protrusion, large overjet, space deficiency and approximately full Class II relationship, distal movement of the maxillary first molars using miniscrew anchorage along with extraction of the maxillary and mandibular first premolars was …

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Using Orthognathic Surgery And Prostheses To Improve Esthetics And Occlusion Of Skeletal Class Iii Patient, Hsiao-Ting Huang, Ya-Yi Chen, Po-Hsun Chen, Yu-Ray Chen, Yu-Hwa Pan, Ting-Ting Wu Aug 2022

Using Orthognathic Surgery And Prostheses To Improve Esthetics And Occlusion Of Skeletal Class Iii Patient, Hsiao-Ting Huang, Ya-Yi Chen, Po-Hsun Chen, Yu-Ray Chen, Yu-Hwa Pan, Ting-Ting Wu

Taiwanese Journal of Orthodontics

Patient with class III malocclusion and anterior crossbite combined with mandibular prognathism with deviation can be corrected through orthognathic surgery combined with conventional orthodontics. A proper diagnosis and careful treatment planning are the keys to success. This case report described a 30-yearold female, who complained of poor esthetics because of the square face and dark teeth. After a routine orthodontic examination, the diagnosis was skeletal class III malocclusion with mandibular prognathism and a deviated chin shifted to the right. The treatment plan was 2 jaw orthognathic surgery combined with genioplasty and gonioplasty to improve the deficient upper incisal show, protrusive …

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Knowing When To Fold'em: A Monte Carlo Exploration Of Card Shuffling And How Poker Players Can Gain An Advantage, David W. Merz, Peter B. Chi Aug 2022

Knowing When To Fold'em: A Monte Carlo Exploration Of Card Shuffling And How Poker Players Can Gain An Advantage, David W. Merz, Peter B. Chi

UNLV Gaming Research & Review Journal

This work demonstrates that the card shuffling procedure commonly performed in casino poker rooms is insufficient for randomizing a deck of cards. We explore this in the context of Texas Hold’em, which has established itself as the most popular form of poker worldwide over the past few decades. We show the degree to which the resulting distribution of the orderings of all 52 cards in the deck after shuffling is not uniform. Rather, any given card may be substantially more (or less) likely to show up as an important card in the subsequent hand. Additionally, we find that the shuffling …

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Computational Study On The Effectiveness Of Flavonoids From Marsilea Crenata C. Presl As Potent Sirt1 Activators And Nfκb Inhibitors, Sri Rahayu, Sasangka Prasetyawan, Sri Widyarti, Mochammad Fitri Atho’Illah, Gatot Ciptadi Aug 2022

Computational Study On The Effectiveness Of Flavonoids From Marsilea Crenata C. Presl As Potent Sirt1 Activators And Nfκb Inhibitors, Sri Rahayu, Sasangka Prasetyawan, Sri Widyarti, Mochammad Fitri Atho’Illah, Gatot Ciptadi

Karbala International Journal of Modern Science

Ovarian aging is a natural process in females, and it occurs due to an elevated ROS-induced inflammation caused by oxidative stress. SIRT-1 is a metabolic sensor that tightly regulates oxidative and inflammatory responses. However, this regulative function is antagonized by NFκB. Therefore, the objective of this study was to explore the pathways involved in aging and identify the flavonoid compounds from Marsilea crenata that might be useful as SIRT1 activators and NFκB inhibitors. The screening began with exploring the protein-protein interaction in the experimental process using BioGrid, and the role of the flavonoid was evaluated using STITCH. The interaction between …

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Molecular Characterization Of Esbls And Ampc Β-Lactamases In Bacteria Isolated From Currency Notes Circulating In Mosul City, Iraq, Mahmood Zeki Al-Hasso, Shakir Ghazi Gergees, Zahraa Khairialdeen Mohialdeen Aug 2022

Molecular Characterization Of Esbls And Ampc Β-Lactamases In Bacteria Isolated From Currency Notes Circulating In Mosul City, Iraq, Mahmood Zeki Al-Hasso, Shakir Ghazi Gergees, Zahraa Khairialdeen Mohialdeen

Karbala International Journal of Modern Science

The Iraqi currency notes circulating in Mosul city were evaluated for the occurrence of ESBLs and AmpC b-lactamaseproducing bacteria. Four hundred and twenty-two Gram-positive and negative bacterial isolates with different antimicrobial resistance profiles were recovered from 250 samples collected during the period from April to July 2021, among which 150 isolates (35.5%) were multi-drug resistant (MDR). The study found that 16.4% and 14.8% of Gram negative isolates were positive for ESBLs and AmpC phenotypic detection tests, respectively. Interestingly, 6.6% of the isolates were simultaneously positive for both tests. Molecular characterization was carried out using PCR technique to determine the prevalent …

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Molecular Docking And Dynamics Simulation Studies To Predict Multiple Medicinal Plants’ Bioactive Compounds Interaction And Its Behavior On The Surface Of Denv-2 E Protein, Arief Hidayatullah, Wira Eka Putra, Muhaimin Rifa’I, Sustiprijatno Sustiprijatno, Diana Widiastuti, Muhammad Fikri Heikal, Hendra Susanto, Wa Ode Salma, Hilal Mulyadi Aug 2022

Molecular Docking And Dynamics Simulation Studies To Predict Multiple Medicinal Plants’ Bioactive Compounds Interaction And Its Behavior On The Surface Of Denv-2 E Protein, Arief Hidayatullah, Wira Eka Putra, Muhaimin Rifa’I, Sustiprijatno Sustiprijatno, Diana Widiastuti, Muhammad Fikri Heikal, Hendra Susanto, Wa Ode Salma, Hilal Mulyadi

Karbala International Journal of Modern Science

The envelope protein (E) is a fusion class II protein that is essential for DENV fusion. We use two active compounds derived from commonly used plants in Indonesia: galangin and kaempferide. We ran a docking and 1000 ps molecular dynamic analysis with normal physiological parameters. During the simulation, galangin and kaempferide binding sites fluctuated. But chloroquine has lesser ligand mobility, hence keeping contact with fusion loops, whereas both drugs lose contact with hydrophobic pockets. However, the two active compounds have a more stable ligand configuration. Less than 2 Å alterations were seen in the RMSF simulation of the protein E …

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Effects Of Spatially Heterogeneous Lakeside Development On Nearshore Biotic Communities In A Large, Deep, Oligotrophic Lake, Michael F. Meyer, Ted Ozersky, Kara H. Woo, Kirill Shchapov, Aaron W. E. Galloway, Julie B. Schram, Emma J. Rosi, Daniel D. Snow, Maxim A. Timofeyev, Dmitry Yu. Karnaukhov, Matthew R. Brousil, Stephanie E. Hampton Aug 2022

Effects Of Spatially Heterogeneous Lakeside Development On Nearshore Biotic Communities In A Large, Deep, Oligotrophic Lake, Michael F. Meyer, Ted Ozersky, Kara H. Woo, Kirill Shchapov, Aaron W. E. Galloway, Julie B. Schram, Emma J. Rosi, Daniel D. Snow, Maxim A. Timofeyev, Dmitry Yu. Karnaukhov, Matthew R. Brousil, Stephanie E. Hampton

School of Natural Resources: Faculty Publications

Sewage released from lakeside development can reshape ecological communities. Nearshore periphyton can rapidly assimilate sewage-associated nutrients, leading to increases of filamentous algal abundance, thus altering both food abundance and quality for grazers. In Lake Baikal, a large, ultra-oligotrophic, remote lake in Siberia, filamentous algal abundance has increased near lakeside developments, and localized sewage input is the suspected cause. These shifts are of particular interest in Lake Baikal, where endemic littoral biodiversity is high, lakeside settlements are mostly small, tourism is relatively high (~1.2 million visitors annually), and settlements are separated by large tracts of undisturbed shoreline, enabling investigation of heterogeneity …

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Alfalfa Establishment And Management In South Carolina, Liliane Silva, Michael Mrshall, Jeremy Greene, Matias Aguerre Aug 2022

Alfalfa Establishment And Management In South Carolina, Liliane Silva, Michael Mrshall, Jeremy Greene, Matias Aguerre

Livestock and Forages

Alfalfa is a perennial legume with high forage quality that can be used under grazing or hay management. This publication outlines establishment and management recommendations for alfalfa plantings in South Carolina.

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Modeling Tunable Fracture In Hydrogel Shell Structures For Biomedical Applications, Gang Zhang, Hai Qiu, Khalil I. Elkhodary, Shan Tang Aug 2022

Modeling Tunable Fracture In Hydrogel Shell Structures For Biomedical Applications, Gang Zhang, Hai Qiu, Khalil I. Elkhodary, Shan Tang

Faculty Journal Articles

No abstract provided.

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Ud Biologist Named Fellow In National Leadership Program For Stem Women, University Of Dayton Aug 2022

Ud Biologist Named Fellow In National Leadership Program For Stem Women, University Of Dayton

College of Arts and Sciences Newsroom

University of Dayton biologist Yvonne Sun was selected for Drexel University’s Executive Leadership in Academic Technology, Engineering and Science.

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The Contemporary Tax Journal Volume 11, No. 2 – Summer 2022 Aug 2022

The Contemporary Tax Journal Volume 11, No. 2 – Summer 2022

The Contemporary Tax Journal

No abstract provided.

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Understanding Across The Senses: Cross-Modal Studies Of Cognition In Cetaceans, Jason N. Bruck, Adam A. Pack Aug 2022

Understanding Across The Senses: Cross-Modal Studies Of Cognition In Cetaceans, Jason N. Bruck, Adam A. Pack

Faculty Publications

Cross-modal approaches to the study of sensory perception, social recognition, cognition, and mental representation have proved fruitful in humans as well as in a variety of other species including toothed whales in revealing equivalencies that suggest that different sensory stimuli associated with objects or individuals may effectively evoke mental representations that are, respectively, object based or individual based. Building on established findings of structural equivalence in the form of spontaneous recognition of complex shapes across the modalities of echolocation and vision and behavior favoring identity echoic–visual cross-modal relationships over associative echoic–visual cross-modal relationships, examinations of transitive inference equivalencies from initially …

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The Contemporary Tax Journal’S Interview With Ms. Myra Sutanto Shen, Dale Loepp Cpa Aug 2022

The Contemporary Tax Journal’S Interview With Ms. Myra Sutanto Shen, Dale Loepp Cpa

The Contemporary Tax Journal

No abstract provided.

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August 18-19, 2022, Ohio University Board Of Trustees Meeting Minutes, Ohio University Board Of Trustees Aug 2022

August 18-19, 2022, Ohio University Board Of Trustees Meeting Minutes, Ohio University Board Of Trustees

Board of Trustees Meeting Minutes

Meeting minutes document the activities of Ohio University's Board of Trustees.

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Examining Victim-Worthiness And The Court Systems In Massachusetts Domestic Abuse Cases, Natalie Johns-Suriel Aug 2022

Examining Victim-Worthiness And The Court Systems In Massachusetts Domestic Abuse Cases, Natalie Johns-Suriel

Honors Program Theses and Projects

This study investigates the relationship between victims of intimate partner violence and legal actors within the Commonwealth of Massachusetts. Qualitative interviews were conducted with nine participants, eight were advocates for victims and one was an attorney for victims at an advocacy center. Interviewees recall their experience aiding victims both in and out of the courtroom, noting differences depending on how the victim’s race, gender, or sexuality interact with social structures and legal actors. Narratives provided by advocates reveal a difference in treatment for victims of domestic abuse depending on their social categories, including race, gender, ethnicity, sexuality, etc. My analysis …

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Let’S Play! The Power Of Play As A Teaching Tool In The Elementary And Early Childhood Classroom, Jessica Moore Aug 2022

Let’S Play! The Power Of Play As A Teaching Tool In The Elementary And Early Childhood Classroom, Jessica Moore

Honors Program Theses and Projects

What play-based learning looks like depends on the subject it is being used in. Overall, it involves hands-on activities and children learning through these activities. These activities can be used to teach/introduce the lesson, or they can be used as a reinforcement. In science, it might be experimenting with cardboard and cars to understand ramps. For math, it might be using manipulatives to learn about shapes. In social studies it could be putting on a puppet show, while in English it might be playing Ispy to find words that begin with the letter p. Three research questions were created to …

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The Haunting Of Wright Manor, Katherine Holzman Aug 2022

The Haunting Of Wright Manor, Katherine Holzman

Honors Program Theses and Projects

The Haunting of Wright Manor explores the fear of the unknown, and more specifically, the hereditary nature of evil. “The phrase ‘nature versus nurture’ was first coined in the mid-1800s by the English Victorian polymath Francis Galton in discussion about the influence of heredity and environment on social advancement”(Serpell 2013), writes Mick Serpell in the British Journal of Pain. Essentially, “Nature versus Nurture” boils down to a debate of which character traits are inherited and which are the result of external environmental factors. Wright Manor’s protagonist, Victoria Mariano, finds herself struggling with the aforementioned debate. At the age of 12, …

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“I’M Not Just Made For Men”: How Online Sex Workers Manage Misogyny, Sarah Dellner Aug 2022

“I’M Not Just Made For Men”: How Online Sex Workers Manage Misogyny, Sarah Dellner

Honors Program Theses and Projects

Online sex work, or erotic labor, refers to sexual services that are provided via the Internet for compensation. Views of sex work range from empowering and agentic for sex workers, rife with violence and victimization. Various frameworks (Jones, 2016 Vance, 1984; Weitzer, 2010) examine the balance between these extremes; but often lacks nuance. I examined female and non-binary online sex workers’ experiences with misogyny and how they cope with it. Zoom interviews were conducted with 15 participants ranging from 18 to 33 years old. Using an intersectional feminist lens and thematic analysis (Braun & Clarke, 2020), two overarching themes were …

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Establishing The Freestyle 293 Expression System As A Tool To Generate Recombinant Apolipoprotein E In Mammalian Cells, Michaela Cunningham Aug 2022

Establishing The Freestyle 293 Expression System As A Tool To Generate Recombinant Apolipoprotein E In Mammalian Cells, Michaela Cunningham

Honors Program Theses and Projects

Alzheimer’s disease (AD) is a devastating form of dementia with 5 million people suffering from the disease in the United States alone. Thus, there is a need to understand the cause of AD so that therapeutics can be developed to treat it. The greatest risk factor for AD is the APOE gene, which encodes the apolipoprotein Eprotein. Three predominant APOE alleles exist humans, which give rise to three isoforms, called apoE2, E3, and E4. Individuals with apoE4 are ten times more likely to develop AD. In contrast, the apoE2 isoform has a neuroprotective effect and is associated with a 2-fold …

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Characterization Of Exosomes And Their Role In Cellular Migration In Chemosensitive And Chemoresistant Ovarian Cancer Cells, Courtney Mceneaney Aug 2022

Characterization Of Exosomes And Their Role In Cellular Migration In Chemosensitive And Chemoresistant Ovarian Cancer Cells, Courtney Mceneaney

Honors Program Theses and Projects

Chemotherapy-resistant cells that remain after primary cancer treatment lead to recurrence and metastasis, resulting in a poor prognosis for patients. Chemoresistant cells were created by our collaborators using the human ovarian cancer cell line, OVCAR8, by selection of cells that survived serial culture with cisplatin, a platinum-based chemotherapeutic. The cisplatin-resistant cells (OVCAR8R) demonstrate a morphology unique from the original cisplatin-sensitive cells (OVCAR8) which could correlate with their difference in aggressiveness. My research goal is to isolate and characterize exosomes from the OVCAR8 and OVCAR8Rlines. Exosomes are small vesicles secreted from cells and contribute to the tumor microenvironment(TME). It has been …

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