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2022

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Assessment During Covid-19: Quality Assurance Of An Online Open Book Formative Examination For Undergraduate Medical Students, Javeria Rehman, Rahila Ali Mazhar, Azam Afzal, Sara Shakil, Amber Shahmim Sultan, Romana Idress, Syeda Sadia Fatima Nov 2022

Assessment During Covid-19: Quality Assurance Of An Online Open Book Formative Examination For Undergraduate Medical Students, Javeria Rehman, Rahila Ali Mazhar, Azam Afzal, Sara Shakil, Amber Shahmim Sultan, Romana Idress, Syeda Sadia Fatima

Department of Pathology and Laboratory Medicine

Background: The spread of COVID-19 pandemic in early 2020 compelled all the educational activities, including medical education to be shifted from face-to-face interaction to a virtual platform. This shift provided opportunities for exploring online assessment modalities. One such assessment method is an online open book exam which is a unique concept in medical education of Pakistan. Limited information is available in literature regarding open book exam for the basic science subjects. Hence, the objective of the study was to determine the quality of the open book exam administered as a pilot project to the first-year medical students.
Methods: It was …


Relationship Between Individual Characteristics And Received Social Support Among People With Serious Mental Illness, Samantha Dicorte Nov 2022

Relationship Between Individual Characteristics And Received Social Support Among People With Serious Mental Illness, Samantha Dicorte

USF Tampa Graduate Theses and Dissertations

Past research on social support has mainly focused on perceived social support. Relatively less research has been done on received social support and how it can promote positive behavior outcomes. This study examines the importance of received social support, including background on social support, social support outcomes, and a theoretical foundation overview of the social stress model. In addition to a literature review, this study investigates the association between individual characteristics (i.e., diagnosis and substance abuse including alcohol, race/ethnicity, gender, age, and marital status) and different types of received social support (i.e., daily, mental health, and adherence support). Simple linear …


Leveraging Smart-Phone Cameras And Image Processing Techniques To Classify Mosquito Genus And Species, Sriram Chellappan, Pratool Bharti, Mona Minakshi, Willie Mcclinton, Jamshidbek Mirzakhalov Nov 2022

Leveraging Smart-Phone Cameras And Image Processing Techniques To Classify Mosquito Genus And Species, Sriram Chellappan, Pratool Bharti, Mona Minakshi, Willie Mcclinton, Jamshidbek Mirzakhalov

USF Patents

Identifying insect species integrates image processing, feature selection, unsupervised clustering, and a support vector machine (SVM) learning algorithm for classification. Results with a total of 101 mosquito specimens spread across nine different vector carrying species demonstrate high accuracy in species identification. When implemented as a smart-phone application, the latency and energy consumption were minimal. The currently manual process of species identification and recording can be sped up, while also minimizing the ensuing cognitive workload of personnel. Citizens at large can use the system in their own homes for self-awareness and share insect identification data with public health agencies.


Alteration In Tyrosine Phosphorylation Of Cardiac Proteome And Egfr Pathway Contribute To Hypertrophic Cardiomyopathy, Mingguo Xu, Kevin C. Bermea, Marzieh Ayati, Han Byeol Yang, Xiaomei Yang, Andres Medina, Zongming Fu, Amir Heravi, Xinyu Zhang, Genaro A. Ramirez-Correa Nov 2022

Alteration In Tyrosine Phosphorylation Of Cardiac Proteome And Egfr Pathway Contribute To Hypertrophic Cardiomyopathy, Mingguo Xu, Kevin C. Bermea, Marzieh Ayati, Han Byeol Yang, Xiaomei Yang, Andres Medina, Zongming Fu, Amir Heravi, Xinyu Zhang, Genaro A. Ramirez-Correa

School of Medicine Publications

Alterations of serine/threonine phosphorylation of the cardiac proteome are a hallmark of heart failure. However, the contribution of tyrosine phosphorylation (pTyr) to the pathogenesis of cardiac hypertrophy remains unclear. We use global mapping to discover and quantify site-specific pTyr in two cardiac hypertrophic mouse models, i.e., cardiac overexpression of ErbB2 (TgErbB2) and α myosin heavy chain R403Q (R403Q-αMyHC Tg), compared to control hearts. From this, there are significant phosphoproteomic alterations in TgErbB2 mice in right ventricular cardiomyopathy, hypertrophic cardiomyopathy (HCM), and dilated cardiomyopathy (DCM) pathways. On the other hand, R403Q-αMyHC Tg mice indicated that the EGFR1 pathway is central for …


The Role Of Environmental Awareness In Preserving Domestic Water From Waste In Kufa District, Lamia Abdel Taha, Sara Abdel Taha, Sinai Abdel Taha Nov 2022

The Role Of Environmental Awareness In Preserving Domestic Water From Waste In Kufa District, Lamia Abdel Taha, Sara Abdel Taha, Sinai Abdel Taha

Uruk Journal of Human Sciences

The trends around the concept of water rationalization have evolved significantly and are no longer limited to the economic aspect and the physical, chemical and biological characterization of water properties, but include the social aspect that examines the human behavior of water use and the role of gender in providing water and reducing consumption and water waste.


That's A Wrap: Using Cultural Discourse Analysis To Explicate The Film Portrayal Of Communication Between Elderly Parents And Their Adult Child(Ren) In A Caregiving Relationship, Gerri A. Duran Nov 2022

That's A Wrap: Using Cultural Discourse Analysis To Explicate The Film Portrayal Of Communication Between Elderly Parents And Their Adult Child(Ren) In A Caregiving Relationship, Gerri A. Duran

Communication ETDs

There are burgeoning numbers of elderly and a significant number of their adult children provide them with some level of care. To date, there has been little research on elderly parents’ perspective as they communicate with their adult children about the care provided by their children. This dissertation addresses this gap by identifying the nature of the communication, as portrayed in film, which occurs between an elderly parent (EP) and their caregiving adult child (AC).

In addition to identifying the nature of communication between an EP and their AC, I examine the similarities/differences of their communication among the films. I …


The Dance Of Domesticity: How Gender Constructs Obscure Lived Experience At Museums, Marcy J. Botwick Nov 2022

The Dance Of Domesticity: How Gender Constructs Obscure Lived Experience At Museums, Marcy J. Botwick

Museum Studies Theses

My thesis focuses on Mary Shepard Greene Blumenschein and Ernest L. Blumenschein, married artists born in the late 1860s. Ernest Blumenschein was an important regional artist and member of the Taos Society of Artists (TSA). Paintings by Blumenschein and other TSA members promoted tourism in the Southwestern United States through annual exhibitions and their use in advertising the Atchison, Topeka and Santa Fe Railway (AT&SF). Mary Greene Blumenschein was an award-winning painter and illustrator whose work focused on images of women at the beginning of the twentieth century, however, she is now a secondary and obscure figure in art history. …


Status Seeking In Conspicuous Spectator Sports Consumption: A Self-Determination Theory Perspective, Yong Wang Nov 2022

Status Seeking In Conspicuous Spectator Sports Consumption: A Self-Determination Theory Perspective, Yong Wang

Health, Exercise, and Sports Sciences ETDs

According to self-determination theory, people are intrinsically and/or extrinsically motivated to self-regulate their behavior (Deci & Ryan, 2012). Sport fans watch games for intrinsic rewards such as having fun and supporting a team, which, from the identity fusion perspective (Swann Jr et al., 2009), derive from their visceral sense of oneness with a team. Although they can choose to watch games at home, many fans spend money attending expensive games (e.g., major sports) and publicly display their experience. A plethora of sport management literature has addressed the intrinsic motivation for attending games; however, less attention has been paid to the …


Rppa Space: An R Package For Normalization And Quantitation Of Reverse-Phase Protein Array Data, Huma Shehwana, Shwetha V Kumar, James M Melott, Mary A Rohrdanz, Chris Wakefield, Zhenlin Ju, Doris R Siwak, Yiling Lu, Bradley M Broom, John N Weinstein, Gordon B Mills, Rehan Akbani Nov 2022

Rppa Space: An R Package For Normalization And Quantitation Of Reverse-Phase Protein Array Data, Huma Shehwana, Shwetha V Kumar, James M Melott, Mary A Rohrdanz, Chris Wakefield, Zhenlin Ju, Doris R Siwak, Yiling Lu, Bradley M Broom, John N Weinstein, Gordon B Mills, Rehan Akbani

Faculty, Staff and Student Publications

SUMMARY: Reverse-Phase Protein Array (RPPA) is a robust high-throughput, cost-effective platform for quantitatively measuring proteins in biological specimens. However, converting raw RPPA data into normalized, analysis-ready data remains a challenging task. Here, we present the RPPA SPACE (RPPA Superposition Analysis and Concentration Evaluation) R package, a substantially improved successor to SuperCurve, to meet that challenge. SuperCurve has been used to normalize over 170 000 samples to date. RPPA SPACE allows exclusion of poor-quality samples from the normalization process to improve the quality of the remaining samples. It also features a novel quality-control metric, 'noise', that estimates the level of random …


Transposon Mutagenesis Reveals Rbms3 Silencing As A Promoter Of Malignant Progression Of Brafv600e-Driven Lung Tumorigenesis, Aria Vaishnavi, Joseph Juan, Maebh Jacob, Christopher Stehn, Eric E Gardner, Michael T Scherzer, Sophia Schuman, J Edward Van Veen, Brandon Murphy, Christopher S Hackett, Adam J Dupuy, Steven A Chmura, Louise Van Der Weyden, Justin Y Newberg, Annie Liu, Karen Mann, Alistair G Rust, William A Weiss, Conan G Kinsey, David J Adams, Allie Grossmann, Michael B Mann, Martin Mcmahon Nov 2022

Transposon Mutagenesis Reveals Rbms3 Silencing As A Promoter Of Malignant Progression Of Brafv600e-Driven Lung Tumorigenesis, Aria Vaishnavi, Joseph Juan, Maebh Jacob, Christopher Stehn, Eric E Gardner, Michael T Scherzer, Sophia Schuman, J Edward Van Veen, Brandon Murphy, Christopher S Hackett, Adam J Dupuy, Steven A Chmura, Louise Van Der Weyden, Justin Y Newberg, Annie Liu, Karen Mann, Alistair G Rust, William A Weiss, Conan G Kinsey, David J Adams, Allie Grossmann, Michael B Mann, Martin Mcmahon

Faculty, Staff and Student Publications

Mutationally activated BRAF is detected in approximately 7% of human lung adenocarcinomas, with BRAFT1799A serving as a predictive biomarker for treatment of patients with FDA-approved inhibitors of BRAFV600E oncoprotein signaling. In genetically engineered mouse (GEM) models, expression of BRAFV600E in the lung epithelium initiates growth of benign lung tumors that, without additional genetic alterations, rarely progress to malignant lung adenocarcinoma. To identify genes that cooperate with BRAFV600E for malignant progression, we used Sleeping Beauty-mediated transposon mutagenesis, which dramatically accelerated the emergence of lethal lung cancers. Among the genes identified was Rbms3, which encodes an RNA-binding protein previously implicated as a …


Preclinical Evaluation Of Combination Nemtabrutinib And Venetoclax In Chronic Lymphocytic Leukemia, Elizabeth M Muhowski, Janani Ravikrishnan, Britten Gordon, Lianbo Yu, Shrilekha Misra, Brandi Walker, Sudharshan Eathiraj, Deepa Sampath, Kerry A Rogers, John C Byrd, Jennifer A Woyach Nov 2022

Preclinical Evaluation Of Combination Nemtabrutinib And Venetoclax In Chronic Lymphocytic Leukemia, Elizabeth M Muhowski, Janani Ravikrishnan, Britten Gordon, Lianbo Yu, Shrilekha Misra, Brandi Walker, Sudharshan Eathiraj, Deepa Sampath, Kerry A Rogers, John C Byrd, Jennifer A Woyach

Faculty, Staff and Student Publications

Inhibitors of B cell receptor (BCR) signaling such as the Bruton's tyrosine kinase (BTK) inhibitors are effective therapeutics for chronic lymphocytic leukemia (CLL). The first-in-class covalent BTK inhibitor, ibrutinib, produces durable responses in most CLL patients; however, complete responses are only observed in a minority of patients. B cell lymphoma 2 (BCL2), an anti-apoptotic protein that contributes to CLL cell survival, has also been investigated as a therapeutic target. The BCL2 inhibitor venetoclax is effective in patients with CLL and can produce undetectable minimal residual disease, allowing discontinuation of therapy. In combination, ibrutinib and venetoclax have shown preclinical synergy and …


Las Ciudades Del Deseo: Las Políticas De Género, Sexualidad Y Espacio Urbano En El Caribe Hispano, Elena Valdez Nov 2022

Las Ciudades Del Deseo: Las Políticas De Género, Sexualidad Y Espacio Urbano En El Caribe Hispano, Elena Valdez

Purdue Studies in Romance Literatures

Las ciudades del deseo explores the representations of gender, sexuality, and urban space in contemporary narratives from Cuba, the Dominican Republic, and Puerto Rico. By examining a corpus of novels published since 2000, this book shows how the changes in urban landscape create a new image of the city that destroys traditional gender roles and produces different discourses on sexuality. At moments of crisis in political agendas that took place between 1990 and 2000, queer subjects became spokespeople outlining new national projects on each island, while claiming space in the national imaginary. The nation is no longer built on blood …


Taxonomic Revision And Morphometric Analysis Of Selected Anthurium (Araceae) Species From Bolivia And Peru, Daniel M. Tarazona Ocana Nov 2022

Taxonomic Revision And Morphometric Analysis Of Selected Anthurium (Araceae) Species From Bolivia And Peru, Daniel M. Tarazona Ocana

Theses

The genus Anthurium (Araceae) consists of around 1,200 species distributed in the Neotropics, particularly in Central and South America. The montane forests in the eastern flank of the Andes, ranging from northern Ecuador to central Bolivia; hereafter, the East Andes Gradient region, are particularly rich in terms of species diversity, and include an understudied group of Anthurium species endemic to the region. Within this group of species, some taxa are difficult to distinguish from each other mainly due to the lack of identification keys, incomplete species descriptions and unknown synonyms. In this study, a combination of traditional taxonomic techniques and …


Enabling Nato’S Collective Defense: Critical Infrastructure Security And Resiliency (Nato Coe-Dat Handbook 1), Carol V. Evans, Chris Anderson, Malcom Baker, Ronald Bearse, Salih Biçakci, Steve Bieber, Sungbaek Cho, Adrian Dwyer, Geoffrey French, David Harell, Alessandro Lazari, Raymond Mey, Theresa Sabonis-Helf, Duane Verner Nov 2022

Enabling Nato’S Collective Defense: Critical Infrastructure Security And Resiliency (Nato Coe-Dat Handbook 1), Carol V. Evans, Chris Anderson, Malcom Baker, Ronald Bearse, Salih Biçakci, Steve Bieber, Sungbaek Cho, Adrian Dwyer, Geoffrey French, David Harell, Alessandro Lazari, Raymond Mey, Theresa Sabonis-Helf, Duane Verner

Books, Monographs & Collaborative Studies

In 2014 NATO’s Centre of Excellence-Defence Against Terrorism (COE-DAT) launched the inaugural course on “Critical Infrastructure Protection Against Terrorist Attacks.” As this course garnered increased attendance and interest, the core lecturer team felt the need to update the course in critical infrastructure (CI) taking into account the shift from an emphasis on “protection” of CI assets to “security and resiliency.” What was lacking in the fields of academe, emergency management, and the industry practitioner community was a handbook that leveraged the collective subject matter expertise of the core lecturer team, a handbook that could serve to educate government leaders, state …


Polyphenolics, Glucosinolates And Isothiocyanates Profiling Of Aerial Parts Of Nasturtium Officinale (Watercress), Sotiris Kyriakou, Kyriaki Michailidou, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis Nov 2022

Polyphenolics, Glucosinolates And Isothiocyanates Profiling Of Aerial Parts Of Nasturtium Officinale (Watercress), Sotiris Kyriakou, Kyriaki Michailidou, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

Nebraska Center for Virology: Faculty Publications

Watercress (Nasturtium officinale) is a rich source of secondary metabolites with disease-preventing and/or health-promoting properties. Herein, we have utilized extraction procedures to isolate fractions of polyphenols, glucosinolates and isothiocyanates to determine their identification, and quantification. In doing so, we have utilized reproducible analytical methodologies based on liquid chromatography with tandem mass spectrometry by either positive or negative ion mode. Due to the instability and volatility of isothiocyanates, we followed an ammonia derivatization protocol which converts them into respective ionizable thiourea derivatives. The analytes’ content distribution map was created on watercress flowers, leaves and stems. We have demonstrated that …


Polyphenolics, Glucosinolates And Isothiocyanates Profiling Of Aerial Parts Of Nasturtium Officinale (Watercress), Sotiris Kyriakou, Kyriaki Michailidou, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis Nov 2022

Polyphenolics, Glucosinolates And Isothiocyanates Profiling Of Aerial Parts Of Nasturtium Officinale (Watercress), Sotiris Kyriakou, Kyriaki Michailidou, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Watercress (Nasturtium officinale) is a rich source of secondary metabolites with disease-preventing and/or health-promoting properties. Herein, we have utilized extraction procedures to isolate fractions of polyphenols, glucosinolates and isothiocyanates to determine their identification, and quantification. In doing so, we have utilized reproducible analytical methodologies based on liquid chromatography with tandem mass spectrometry by either positive or negative ion mode. Due to the instability and volatility of isothiocyanates, we followed an ammonia derivatization protocol which converts them into respective ionizable thiourea derivatives. The analytes’ content distribution map was created on watercress flowers, leaves and stems. We have demonstrated that …


Study Design And Baseline Characteristics For The Reflect Gene Therapy Trial Ofm.11778g>A/Nd4-Lhon, Prem S. Subramanian, Nancy J. Newman, Mark Moster, An-Guor Wang, Patrick Yu-Wai-Man, Sean Donahue, Bart P. Leroy, Valerio Carelli, Valerie Biousse, Catherine Vignal-Clermont, Robert C. Sergott, Alfredo A. Sadun, Gema Rebolleda, Bart K. Chwalisz, Rudrani Banik, Fabienne Bazin, Eric Cox, Michel Roux, Magali Taiel, Jose-Alain Sahel Nov 2022

Study Design And Baseline Characteristics For The Reflect Gene Therapy Trial Ofm.11778g>A/Nd4-Lhon, Prem S. Subramanian, Nancy J. Newman, Mark Moster, An-Guor Wang, Patrick Yu-Wai-Man, Sean Donahue, Bart P. Leroy, Valerio Carelli, Valerie Biousse, Catherine Vignal-Clermont, Robert C. Sergott, Alfredo A. Sadun, Gema Rebolleda, Bart K. Chwalisz, Rudrani Banik, Fabienne Bazin, Eric Cox, Michel Roux, Magali Taiel, Jose-Alain Sahel

Wills Eye Hospital Papers

Objective REFLECT is the first randomised, double-masked, placebo-controlled multicentre phase 3 clinical trial that evaluated the efficacy and safety of bilateral intravitreal (IVT) injection of lenadogene nolparvovec in subjects with Leber hereditary optic neuropathy carrying the m.11778G>A mutation.

Methods and analysis A total of 98 subjects were enrolled with vision loss of ≤12 months. The subjects were randomised to one of two treatment arms with all subjects receiving an intravitreal (IVT) injection of lenadogene nolparvovec in their first affected eye and the second-affected eye randomised to receive IVT of either lenadogene nolparvovec or placebo.

Results The majority of subjects …


Serum Ctrp9 And High-Molecular Weight Adiponectin Are Associated With Ischemic Stroke., Yan-Qing Zhang, Yan-Wei Zhang, Jian-Li Dai, Chen Li, Wen-Qing Wang, Hai-Feng Zhang, Wayne Bond Lau, Xiao-Ming Wang, Xiao-Gang Liu, Rong Li Nov 2022

Serum Ctrp9 And High-Molecular Weight Adiponectin Are Associated With Ischemic Stroke., Yan-Qing Zhang, Yan-Wei Zhang, Jian-Li Dai, Chen Li, Wen-Qing Wang, Hai-Feng Zhang, Wayne Bond Lau, Xiao-Ming Wang, Xiao-Gang Liu, Rong Li

Department of Emergency Medicine Faculty Papers

BACKGROUND: C1q/TNF-related protein 9 (CTRP9) and adiponectin (APN) have beneficial metabolic regulatory and vasoprotective effects. This study explored alteration of CTRP9 and APN multimers during onset of ischemic stroke and development, to provide novel clinical and experimental basis for recognition and prevention of ischemic stroke.

METHODS: There were 269 patients with ischemic stroke and 182 control subjects included in this study. Serum levels of CTRP9 and APN multimers in different disease stages were measured.

RESULTS: Serum CTRP9, total APN (tAPN), and high-molecular weight (HMW) APN decreased gradually in stage I (acute stage, within 72 h of onset) of ischemic stroke …


The Prospector, November 15, 2022, Utep Student Publications Nov 2022

The Prospector, November 15, 2022, Utep Student Publications

The Prospector

Headline: Battle of I-10 at the Don Haskins


Fire History And Long-Term Carbon Accumulation In Hemi-Boreal Peatlands Companion Dataset, Dominic Uhelski, Evan Kane, Katherine Heckman, Rodney Chimner Nov 2022

Fire History And Long-Term Carbon Accumulation In Hemi-Boreal Peatlands Companion Dataset, Dominic Uhelski, Evan Kane, Katherine Heckman, Rodney Chimner

Michigan Tech Research Data

This dataset contains peat property data including location, depth, bulk density, organic matter content, and carbon content, infrared spectra, and radiocarbon dates. Peat cores were collected between 2011 and 2019. Analyses were performed between 2018 and 2021. Samples were collected from peatlands in Michigan, Wisconsin, and Minnesota for the purposes of reconstruction of fire history. The data is associated with a yet-to-be-published manuscript to be submitted to Ecosystems.

A README file is included describing the contents of the dataset and all major spreadsheet files contain a Meta worksheet which describes each column of
data.


A Polyamine-Centric, Blood-Based Metabolite Panel Predictive Of Poor Response To Car-T Cell Therapy In Large B Cell Lymphoma, Johannes F Fahrmann, Neeraj Y Saini, Chang Chia-Chi, Ehsan Irajizad, Paolo Strati, Ranjit Nair, Luis E Fayad, Sairah Ahmed, Hun Ju Lee, Swaminathan Iyer, Raphael Steiner, Jody Vykoukal, Ranran Wu, Jennifer B Dennison, Loretta Nastoupil, Preetesh Jain, Michael Wang, Michael Green, Jason Westin, Viktoria Blumenberg, Marco Davila, Richard Champlin, Elizabeth J Shpall, Partow Kebriaei, Christopher R Flowers, Michael Jain, Robert Jenq, Christoph K Stein-Thoeringer, Marion Subklewe, Sattva S Neelapu, Sam Hanash Nov 2022

A Polyamine-Centric, Blood-Based Metabolite Panel Predictive Of Poor Response To Car-T Cell Therapy In Large B Cell Lymphoma, Johannes F Fahrmann, Neeraj Y Saini, Chang Chia-Chi, Ehsan Irajizad, Paolo Strati, Ranjit Nair, Luis E Fayad, Sairah Ahmed, Hun Ju Lee, Swaminathan Iyer, Raphael Steiner, Jody Vykoukal, Ranran Wu, Jennifer B Dennison, Loretta Nastoupil, Preetesh Jain, Michael Wang, Michael Green, Jason Westin, Viktoria Blumenberg, Marco Davila, Richard Champlin, Elizabeth J Shpall, Partow Kebriaei, Christopher R Flowers, Michael Jain, Robert Jenq, Christoph K Stein-Thoeringer, Marion Subklewe, Sattva S Neelapu, Sam Hanash

Faculty, Staff and Student Publications

Anti-CD19 chimeric antigen receptor (CAR) T cell therapy for relapsed or refractory (r/r) large B cell lymphoma (LBCL) results in durable response in only a subset of patients. MYC overexpression in LBCL tumors is associated with poor response to treatment. We tested whether an MYC-driven polyamine signature, as a liquid biopsy, is predictive of response to anti-CD19 CAR-T therapy in patients with r/r LBCL. Elevated plasma acetylated polyamines were associated with non-durable response. Concordantly, increased expression of spermidine synthase, a key enzyme that regulates levels of acetylated spermidine, was prognostic for survival in r/r LBCL. A broad metabolite screen identified …


Theoretical And Experimental Analyses Of Acoustofluidic Phenomena For Biosensing Applications, Pradipta Kr. Das Nov 2022

Theoretical And Experimental Analyses Of Acoustofluidic Phenomena For Biosensing Applications, Pradipta Kr. Das

USF Tampa Graduate Theses and Dissertations

Acoustofluidics is a well-proven tweezer serving various applications such as particle manipulation, cell trapping and separations, fluid mixing, and bio-sensing. In this thesis, we explored several aspects of acoustofluidics such as acoustic streaming, acoustothermal heating and acoustophoretic manipulations of micron sized objects, in the context of biosensing applications.

In Chapter 2, inner acoustic streaming for second-order fluids has been studied analytically by employing asymptotic expansions for thin Stokes layer and low acoustic Mach number. In addition, a multiple-time-scale approach has been adopted to separate the primary oscillatory flow and the steady acoustic streaming. The study considers two sample cases: (i) …


Cerebral Blood Flow And Cardiovascular Risk Effects On Resting Brain Regional Homogeneity, Bhim M Adhikari, L Elliot Hong, Zhiwei Zhao, Danny J J Wang, Paul M Thompson, Neda Jahanshad, Alyssa H Zhu, Stefan Holiga, Jessica A Turner, Theo G M Van Erp, Vince D Calhoun, Kathryn S Hatch, Heather Bruce, Stephanie M Hare, Joshua Chiappelli, Eric L Goldwaser, Mark D Kvarta, Yizhou Ma, Xiaoming Du, Thomas E Nichols, Alan R Shuldiner, Braxton D Mitchell, Juergen Dukart, Shuo Chen, Peter Kochunov Nov 2022

Cerebral Blood Flow And Cardiovascular Risk Effects On Resting Brain Regional Homogeneity, Bhim M Adhikari, L Elliot Hong, Zhiwei Zhao, Danny J J Wang, Paul M Thompson, Neda Jahanshad, Alyssa H Zhu, Stefan Holiga, Jessica A Turner, Theo G M Van Erp, Vince D Calhoun, Kathryn S Hatch, Heather Bruce, Stephanie M Hare, Joshua Chiappelli, Eric L Goldwaser, Mark D Kvarta, Yizhou Ma, Xiaoming Du, Thomas E Nichols, Alan R Shuldiner, Braxton D Mitchell, Juergen Dukart, Shuo Chen, Peter Kochunov

Faculty, Staff and Student Publications

Regional homogeneity (ReHo) is a measure of local functional brain connectivity that has been reported to be altered in a wide range of neuropsychiatric disorders. Computed from brain resting-state functional MRI time series, ReHo is also sensitive to fluctuations in cerebral blood flow (CBF) that in turn may be influenced by cerebrovascular health. We accessed cerebrovascular health with Framingham cardiovascular risk score (FCVRS). We hypothesize that ReHo signal may be influenced by regional CBF; and that these associations can be summarized as FCVRS→CBF→ReHo. We used three independent samples to test this hypothesis. A test-retest sample of N = 30 healthy …


Lipid Surfaces And Glutamate Anions Enhance Formation Of Dynamic Biomolecular Condensates Containing Bacterial Cell Division Protein Ftsz And Its Dna-Bound Regulator Slma, Gianfranco Paccione, Miguel Á Robles-Ramos, Carlos Alfonso, Marta Sobrinos-Sanguino, William Margolin, Silvia Zorrilla, Begoña Monterroso, Germán Rivas Nov 2022

Lipid Surfaces And Glutamate Anions Enhance Formation Of Dynamic Biomolecular Condensates Containing Bacterial Cell Division Protein Ftsz And Its Dna-Bound Regulator Slma, Gianfranco Paccione, Miguel Á Robles-Ramos, Carlos Alfonso, Marta Sobrinos-Sanguino, William Margolin, Silvia Zorrilla, Begoña Monterroso, Germán Rivas

Faculty, Staff and Student Publications

Dynamic biomolecular condensates formed by liquid-liquid phase separation can regulate the spatial and temporal organization of proteins, thus modulating their functional activity in cells. Previous studies showed that the cell division protein FtsZ from Escherichia coli formed dynamic phase-separated condensates with nucleoprotein complexes containing the FtsZ spatial regulator SlmA under crowding conditions, with potential implications for condensate-mediated spatiotemporal control of FtsZ activity in cell division. In the present study, we assessed formation of these condensates in the presence of lipid surfaces and glutamate ions to better approximate the E. coli intracellular environment. We found that potassium glutamate substantially promoted the …


Integrated Multi-Trophic Aquaculture Mitigates The Effects Of Ocean Acidification: Seaweeds Raise System Ph And Improve Growth Of Juvenile Abalone, Scott L. Hamilton, Matthew S. Elliott, Maya S. Devries, Jason Adelaars, Maxwell D. Rintoul, Michael H. Graham Nov 2022

Integrated Multi-Trophic Aquaculture Mitigates The Effects Of Ocean Acidification: Seaweeds Raise System Ph And Improve Growth Of Juvenile Abalone, Scott L. Hamilton, Matthew S. Elliott, Maya S. Devries, Jason Adelaars, Maxwell D. Rintoul, Michael H. Graham

Faculty Research, Scholarly, and Creative Activity

Integrated multi-trophic aquaculture (IMTA) has the potential to enhance growth, reduce nutrient loads, and mitigate environmental conditions compared to traditional single-species culture techniques. The goal of this project was to develop a land-based system for the integrated culture of seaweeds and shellfish, to test the efficacy of integrated versus non-integrated designs, and to assess the potential for IMTA to mitigate the effects of climate change from ocean acidification on shellfish growth and physiology. We utilized the red abalone (Haliotis rufescens) and the red seaweed dulse (Devaleraea mollis) as our study species and designed integrated tanks at three different recirculation rates …


In Vitro Liquid Culture Of The Mollusc-Parasitic Nematode Phasmarhabditis (Rhabditida: Rhabditidae), A. Pieterse, S. Haukeland, V. Půža, J. L. Ross, A. P. Malan Nov 2022

In Vitro Liquid Culture Of The Mollusc-Parasitic Nematode Phasmarhabditis (Rhabditida: Rhabditidae), A. Pieterse, S. Haukeland, V. Půža, J. L. Ross, A. P. Malan

All Peer-Reviewed Publications

The success of the mollusc-parasitic nematode, Phasmarhabditis hermaphrodita (Schneider) Andrássy (Rhabditida: Rhabditidae), as a biological control agent in Europe has led to worldwide interest in phasmarhabditids as biocontrol agents. In this study, the mass culture potential of three phasmarhabditids, namely Phasmarhabditis papillosa, Phasmarhabditis kenyaensis and Phasmarhabditis bohemica, was assessed. In addition, ten bacterial candidates, consisting of seven associated with slugs and three associated with entomopathogenic nematodes, were investigated. The bacteria were tested for their ability to cause mortality to Deroceras invadens, as well as to support nematode growth. Initial mortality studies demonstrated that Kluyvera, Aeromonas and Pseudomonas spp. (AP3) caused …


Science, Technology, Engineering, And Mathematics (Stem) Project-Based Learning (Pbl) Education: A New Mexico Case Study For Equity And Inclusion, Kimberly A. Scheerer Nov 2022

Science, Technology, Engineering, And Mathematics (Stem) Project-Based Learning (Pbl) Education: A New Mexico Case Study For Equity And Inclusion, Kimberly A. Scheerer

Teacher Education, Educational Leadership & Policy ETDs

This research addresses how student participation in Science, Technology, Engineering, and Mathematics (STEM) project-based learning (PBL) education activities encourages underrepresented minority student achievement in STEM career field trajectories. Seven New Mexico high school counselors and 12 STEM organization personnel were interviewed during this study. Their responses represent the nuanced professional voices where New Mexico public education intersects with STEM student interest and cultural influence.

For students, STEM PBL can foster deep integration across educational disciplines and enhance STEM career trajectory interest and readiness. STEM education converged with PBL methodologies has the ability to leverage community support while broadening student networks. …


Covid-19 And Stock Market Liquidity: International Evidence, Muhammad Umar, Ghulame Rubbaniy, Amjad Iqbal, Syed Kumail Abbas Rizvi, Yan Xu Nov 2022

Covid-19 And Stock Market Liquidity: International Evidence, Muhammad Umar, Ghulame Rubbaniy, Amjad Iqbal, Syed Kumail Abbas Rizvi, Yan Xu

All Works

This study analyzes the impact of Covid-19 on stock market liquidity of China and four worst hit countries by the pandemic. Using daily data for the stock market illiquidity spanning over July 1, 2019 to July 10, 2020 and the data for new cases and deaths over the period from December 31, 2019 to July 10, 2020, the results of our GARCH analysis show that liquidity in stock markets of all the sampled countries hit hard by the news of the Covid-19 outbreak. We find that for all sampled countries increase in illiquidity due to temporary shocks reverts to long …


A New Species Of Coelometopon Janssens From The Wild Coast Of South Africa (Coleoptera: Hydraenidae), D Bilton, Mc Mlambo Nov 2022

A New Species Of Coelometopon Janssens From The Wild Coast Of South Africa (Coleoptera: Hydraenidae), D Bilton, Mc Mlambo

School of Biological and Marine Sciences

Coelometopon riberae sp. nov. is described from Mkambati Nature Reserve, on the Wild Coast Region of the Eastern Cape Province, South Africa. Notes are provided to distinguish the new species from other members of this strictly madicolous genus.


Urban Nesting Biology, Population Viability And Ecology Of The Willow Flycatcher, Empidonax Traillii, Stephanie Alexandra Byrne Doorly Nov 2022

Urban Nesting Biology, Population Viability And Ecology Of The Willow Flycatcher, Empidonax Traillii, Stephanie Alexandra Byrne Doorly

Dissertations and Theses

Reproductive success, in any species, relies both on the habitat utilized to reproduce and on the successful reproductive output of individual animals within a population. Populations in turn rely on the reproductive fitness of the individual. Whether populations remain stable, grow, or decline ultimately relies on the combined reproductive output of the individuals within that population.

We studied the Willow Flycatcher (Empidonax traillii; henceforth WIFL), a well-studied Neartic-Neotropical migrant passerine whose breeding range extends from the east to west coast of North America. Across much of their range, and in particular in the western regions, breeding population are …