Digital Commons Network^™

The Analysis Of Commercially Available Kratom Products In Richmond, Virginia, James H. Fleming Jr

Master of Science in Forensic Science Directed Research Projects

Kratom is a novel psychoactive substance that has gained popularity within the past ten years. Originating from Southeast Asia, the leaves of the Mitragyna speciosa tree contain two principal alkaloids, mitragynine and 7-hydroxymitragynine, that play a key role in opioid-like effects. Twenty-nine kratom products were obtained from tobacco shops in the Richmond, Virginia area, including powders, teas, capsules, extracts, and a carbonated beverage. Samples were analyzed using Direct Analysis in Real Time-Mass Spectrometry (DART-MS) for kratom alkaloids, labeled ingredients, and other possible organic compounds. Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used to quantitate aluminum, arsenic, copper, iron, magnesium, nickel, …

Using A Non-Destructive Method To Estimate The Number Of Contributors And Predict Dna Yield In Touch Dna Mixtures, Christin Lee

Master of Science in Forensic Science Directed Research Projects

Touch DNA samples are low templates and often are mixtures, making them typically one of the most difficult types of evidence to interpret. Short tandem repeat analysis is a standard method to estimate the number of contributors and DNA yield for each contributor; however, there are some limitations. Applying a non-destructive pre-DNA analysis technique that provides information about the touch DNA may improve the casework process. Antibody probes to hormones such as testosterone and dihydrotestosterone (DHT), and proteins including cytokeratins (CK) inside epidermal cells, have been demonstrated to produce a personalized signature for human touch cell populations. Testosterone, DHT, and …

Development Of A Loop-Mediated Isothermal Amplification (Lamp) Assay For Detection Of Mirnas, Alisha B. Whelan

Master of Science in Forensic Science Directed Research Projects

Body fluid identification serves a vital role in contextualizing biological evidence received in forensic laboratories. While several fluids can be presumptively detected using immunological or catalytic tests, these tests are susceptible to false results and have limited sensitivity. True confirmatory testing is still somewhat limited in forensic laboratories. With the advancement of DNA typing technologies, laboratories can develop DNA profiles from trace amounts of sample, increasing the demand for sensitive and accurate body fluid detection. This project proposes using loop-mediated isothermal amplification (LAMP) as a method to rapidly detect microRNAs (miRNAs) that are differentially expressed in various body fluids with …

Optimization And Comparison Of Methods For Separation Of Spermatozoa From Superabsorbent Polymer-Containing Forensic Evidence, Hannah Wells

Master of Science in Forensic Science Directed Research Projects

Sexual assault is currently one of the most prevalent crimes that affects victims of all ages. Forensic DNA analysts often confirm proof of contact in sexual assault cases through the identification of spermatozoa and subsequent STR profiling. Diapers and other feminine hygiene products, such as maxi pads and ultrathin pads, are types of superabsorbent polymer-containing evidence that complicate the process of DNA analysis due to the trapping of the spermatozoa in the gel-like matrix. In this study, a comparison of methods was performed to determine which extraction technique produces the highest sperm cell and DNA yield and generates a usable …

Stability And Variation Within Donors Of Mirna Markers For Body Fluid Identification, Kelsey R. Price

Master of Science in Forensic Science Directed Research Projects

Traditional enzymatic tests for body fluid identification are prone to false negatives, false positives, and several body fluids do not have confirmatory or reliable presumptive tests. Therefore, molecular-based tests may be more reliable, such as messenger RNA (mRNA), microbial DNA, or microRNA (miRNA) assays.

miRNAs are small, noncoding RNAs whose main function in-vivo is the regulation of protein expression by selectively suppressing the translation of their corresponding mRNAs. They also lack a poly-A tail, and because of this, their small size, and their association with other molecules such as the RISC protein complex, they are more robust than other RNAs, …

Optimization Of A Microfluidic Device For Cell Capture Using Optical Trapping, Samantha C. Pagel

Master of Science in Forensic Science Directed Research Projects

Biological mixtures which result from evidence produced in sexual assault cases are one of the most common types of mixtures observed in forensic biology casework and have contributed to evidence backlogs across the country. In the forensic community, this has highlighted the need for a more efficient cell separation method at the beginning of the DNA analysis workflow than the currently accepted method. One promising novel front-end cell separation technique is optical trapping. In heterogeneous mixtures, optical tweezers have been demonstrated to manipulate and isolate cells of interest from other cell types.

For this research, a range of 5 to …

Impact Of 1,4-Dithiothreitol (Dtt) On Microbial Community Structures Associated With Human Semen Samples, Christian R. Blaise

Master of Science in Forensic Science Directed Research Projects

Body fluid identification (BFID) is one of the most important steps in forensic science as it provides important context to how DNA analysis should be performed on biological samples found at the scene of a crime. Many scientific methods on BFID have been developed over the years with respective benefits and flaws. One emerging method in recent years is microbial signature based BFID. This method uses the same DNA extract that is utilized for human DNA profile generation for microbial analysis. Although DNA is extracted from all body fluid samples without any additional steps, extraction of human DNA from semen …

A Quantifiler Trio-Based Hrm Mixture Screening Assay For The Quantstudio 6 Flex Qpcr Platform, Dayanara A. Torres

Master of Science in Forensic Science Directed Research Projects

At present, the forensic DNA workflow is not capable of providing information about the contributor status (single source vs. mixtures) of evidentiary samples prior to end-point analysis. This shortcoming can exacerbate the challenges inherent to mixtures and low-template DNA samples. Provided additional sample information earlier in the workflow, protocols could be implemented to mitigate these challenges. High-resolution melt (HRM) curve analysis is a technique used to detect genetic variation in DNA fragments and in the last decade has been evaluated for use in differentiation of samples by genotype and/or contributor status. To this end, a proof-of-concept HRM assay using the …

Classification And Differentiation Of Forensically Relevant Cell Populations Using Imaging Flow Cytometry, Arianna D. Decorte

Master of Science in Forensic Science Directed Research Projects

Forensic casework relies heavily on DNA profiles which may be time-consuming to generate and difficult to interpret when biological mixtures are present. Additionally, there are significant challenges in using sub-source data alone to answer the activity-level questions often most pertinent in criminal cases. Source level information can add critical probative value, however, methods that can provide information as to the source tissue of evidentiary cell populations are limited. There remains a need for new methods that can differentiate between and classify various cell populations, particularly for vaginal and epidermal tissue, since there are no conventional serological techniques specific to these …

Determination Of Cotinine Concentration In Urine By Liquid Chromatography Tandem Mass Spectrometry, Allison Beckett

Master of Science in Forensic Science Directed Research Projects

Tobacco use can cause lasting health conditions which can lead to death. Tobacco exposure comes from obvious sources like smoking or chewing tobacco, however these are not the only administration methods. Secondhand smoke exposure can affect even those who choose not to expose themselves to tobacco, which also opens them to the health issues that come along with tobacco use. Tobacco contains many different compounds, one of which is nicotine (Kataoka, 2009). Although nicotine is the most abundant compound in tobacco, it does not stay in the body for long periods of time. Nicotine metabolizes into cotinine which is more …

Assessing The Detectability Of Cannabinoid Analogs (Delta-8 Thc, Delta-10 Thc And Cbd) And Their Major Metabolites In Six Commercial Cannabinoid Urine Screening Kits, Ashley A. Pokhai

Master of Science in Forensic Science Directed Research Projects

Background:

There has been an exponential surge in the presence and use of cannabinoids since the federal legalization of hemp (Agricultural Improvement Act of 2018), which involves the legalization of medical use, adult recreational use, and decriminalization of cannabis in several states. This growth is not only attributed to ∆9-tetrahydrocannabinol (∆9-THC) and cannabidiol (CBD), the most abundant phytocannabinoid components of cannabis and hemp, respectively, but with many other emerging THC analogs. Structurally, these analogs are similar to ∆9-THC, yet very little information is available about their potency and even less information is available regarding their detectability using commercially available cannabinoid …

Facilitating Equitable Access And Retention For Underrepresented Students At The University Of Mary Washington, Michael K. Abelson, Alvin R. Bryant, Marra Hvozdovic

Doctor of Education Capstones

Higher education institutions are facing increasing pressure to find new ways to attract, retain, and graduate the diverse populations of college students. As a result, colleges and universities need to adapt to the changing demographics of students who benefit from more sustained and engaged forms of support that are responsive to their specific social, cultural, and economic backgrounds. This sequential mixed methods study seeks to understand the ways in which the University of Mary Washington serves its underrepresented students in order to develop strategies to enhance the recruitment and retention of Black, Hispanic/Latinx, low-income, and first-generation college students. Building on …

Bloodshed, Baptism, Beer: Racial Capitalism And Settler Colonialism On The Medieval Baltic, Nicholas Salvato

Geography Honors Projects

Scholarly and popular usage of the term “racial capitalism” has increased exponentially over the past decade, but the validity and implications of its use remain hotly contested. The late Cedric Robinson is the undisputed popularizer of this phrase and is referenced widely by both the slogan’s detractors and proponents. Despite this, little work has been done to engage with the core of his argument about racial capitalism: that capitalism is inalienably racial due to the racialism of the medieval European societies that spawned it. Debates over Robinson’s ideas have thus disregarded the substance of his deployment of the phrase and …

Fasst: Fetal Alcohol Spectrum Support Training An Educational Program To Teach Foster And Adoptive Parents About Fetal Alcohol Spectrum Disorder, Carolyn Matthews

Browse all Theses and Dissertations

Fetal Alcohol Spectrum Disorder (FASD) is an umbrella term for a group of diagnoses that result from exposure to alcohol in utero. Alcohol creates a spectrum of impacts on the developing brain, contributing to lifelong physical, cognitive and behavioral implications. The prevalence of children with FASD in the foster care system is approximately 60 per 1000 children (Lange et al., 2013). Prevalence rates are underestimated due to children not being diagnosed or receiving an alternative diagnosis. This relates to various emotional, behavioral, social, and cognitive struggles for children and their families. To best serve this population and their families, it …

The Mechanisms Of Axon Initial Segment Alteration Due To Disrupted Glucose Metabolism: A Potential Link To Cognitive Impairment, Duc Van Minh Nguyen

Browse all Theses and Dissertations

Cognitive impairment is a serious global health issue due to its widely complicated pathologies that are linked to many diseases, including type 2 diabetes. Yet, detailed mechanisms of cognitive impairment associated with type 2 diabetes are unknown. Evidence suggests type 2 diabetes is associated with disrupted glucose metabolism, elevated methylglyoxal, and axon initial segment (AIS) shortening. Subtle changes of AIS could significantly contribute to neuronal dysfunction in neurological diseases (e.g., cognitive impairment). Utilizing in vitro dissociated mouse cortical cultures, we initially discovered elevated methylglyoxal shortens the AIS length of the immunofluorescent marker AnkyrinG (Griggs et al., 2021). This thesis further …

Effects Of Lipin1 Deficiency & Restoration In The Dystrophic Diaphragm, Alexandra Brown

Browse all Theses and Dissertations

Duchenne Muscular Dystrophy (DMD) is an X-linked recessive disorder that is characterized by severe and progressive muscle wasting (Venugopal & Pavlakis, 2021). This disease is caused by a mutation in the largest known human gene which encodes the protein, dystrophin (Gao & McNally, 2015). Dystrophin connects the inner cytoskeleton to the extracellular matrix and is critical for maintaining the structural stability of muscle cells during contraction (Venugopal & Pavlakis, 2021). Mutations to the dystrophin gene result in myocyte membrane instability, contributing to the structural deterioration of the muscle tissue (Venugopal & Pavlakis, 2021). Progressive muscle degeneration and the replacement of …

Analytical-Based Methods For Studying The Interaction Of Human Red Blood Cells With Noble Metal Nanoparticles, Praveen Kumar Alla

Browse all Theses and Dissertations

With the advent of nanotechnology, the potential applications of nanoscale gold in medicine have expanded substantially. Gold’s therapeutic and toxic properties have been widely studied; however, little is known regarding gold’s contribution to oxidative stress in red blood cells (RBCs). This is especially true for recently designed large (L) gold nanorods (GNRs), which possess unmatched theranostic capabilities. Here, we report on the effect of LGNRs at the level of cellular function, and particularly on ion transport and the glutathione (GSH) concentration in human (H) RBCs. Gold has been hypothesized to effectively react with RBCs sulfhydryl groups, thereby causing depletion of …

Drug Supervision, Jacob Schuman

Faculty Scholarship

Critics of harsh drug sentencing laws in the United States typically focus on long prison sentences. But the American criminal justice system also inflicts a significant volume of drug-related punishment through community supervision (probation, parole, and supervised release). Over one million people are under supervision due to a drug conviction, and drug activity is among the most common reasons for violations. In an age of “mass supervision,” community supervision is a major form of drug sentencing and drug policy.

In this Article, I analyze the federal system of supervised release as a form of drug policy. Congress created supervised release …

Decitizenizing Asian Pacific American Women, Shoba Sivaprasad Wadhia, Margaret Hu

Faculty Scholarship

The Page Act of 1875 excluded Asian women immigrants from entering the United States, presuming they were prostitutes. This presumption was tragically replicated in the 2021 Atlanta Massacre of six Asian and Asian American women, reinforcing the same harmful prejudices. This Article seeks to illuminate how the Atlanta Massacre is symbolic of larger forms of discrimination, including the harms of decitizenship. These harms include limited access to full citizenship rights due to legal barriers, restricted cultural and political power, and a lack of belonging. The Article concludes that these harms result from the structure of past and present immigration laws …

Marketing Authorization At The Fda: Paradigms And Alternatives, Adam I. Muchmore

Faculty Scholarship

In many critical industries, the Food and Drug Administration’s (FDA’s) marketing authorization decisions determine the range of products available in the United States. Because of the broad scope of the FDA’s marketing authorization responsibilities, the existing scholarship focuses on individual product categories, or small groups of product categories, regulated by the agency. This Article identifies how the existing literature has overlooked important connections between the FDA’s different marketing authorization programs. These connections suggest both explanations for existing programs and strategies for potential reforms.

The Article sets forth a two-level framework for analyzing the FDA’s marketing authorization role. At the first …

Building Services Engineering January/February 2022

Building Services Engineering

No abstract provided.

Triton Haze Analogs: The Role Of Carbon Monoxide In Haze Formation, Sarah E. Moran, Sarah M. Hörst, Chao He, Michael J. Radke, Joshua A. Sebree, Noam R. Izenberg, Véronique Vuitton, Laurène Flandinet, François Régis Orthous-Daunay, Cédric Wolters

Faculty Publications

Triton is the largest moon of the Neptune system and possesses a thin nitrogen atmosphere with trace amounts of carbon monoxide and methane, making it of similar composition to that of the dwarf planet Pluto. Like Pluto and Saturn's moon Titan, Triton has a haze layer thought to be composed of organics formed through photochemistry. Here, we perform atmospheric chamber experiments of 0.5% CO and 0.2% CH4 in N2 at 90 K and 1 mbar to generate Triton haze analogs. We then characterize the physical and chemical properties of these particles. We measure their production rate, their bulk composition with …

Over-Privileged: Legal Cannabis, Drug Offending & The Right To Family Integrity, Charisa Smith

South Dakota Law Review

No abstract provided.

A Or Z?: An Analysis Of Thom V. Barnett, Gabrielle Unruh

South Dakota Law Review

No abstract provided.

Exploring Specific Predictors Of Psychosis Onset Over A 2-Year Period: A Decision-Tree Model, Jone Bjornestad, Tore Tjora, Johannes H. Langeveld, Helen J. Stain, Inge Joa, Jan Olav Johannessen, Michelle Friedman-Yakoobian, Wenche Ten Velden Hegelstad

Research outputs 2014 to 2021

Aim: The fluctuating symptoms of clinical high risk for psychosis hamper conversion prediction models. Exploring specific symptoms using machine-learning has proven fruitful in accommodating this challenge. The aim of this study is to explore specific predictors and generate atheoretical hypotheses of onset using a close-monitoring, machine-learning approach. Methods: Study participants, N = 96, mean age 16.55 years, male to female ratio 46:54%, were recruited from the Prevention of Psychosis Study in Rogaland, Norway. Participants were assessed using the Structured Interview for Psychosis Risk Syndromes (SIPS) at 13 separate assessment time points across 2 years, yielding 247 specific scores. A machine-learning …

Cancer And Quiescence: Investigating How The Dream Complex And Retinoblastoma Regulate The Cell Cycle, Lydia Rotman

Dissertations, Master's Theses and Master's Reports

An estimated 1.9 million people in the United States will be diagnosed with cancer in 2022. Cancer is characterized by uncontrolled cell growth, resulting from loss-of-function of key cell cycle regulatory proteins. The retinoblastoma protein (pRb) and p130, are two proteins that regulate cellular entry into the cell cycle. Both pRb and p130 repress expression of cell cycle genes, with pRb interacting with and suppressing E2F-DP transcriptional activators and p130 assembling in the DREAM transcriptional repressor complex. When normal cells receive signals to enter the cell cycle, cyclin and cyclin-dependent kinase (CDK) complexes phosphorylate pRb and p130, causing both to …

Assessing The Validity Of An Online Assessment Of Motor Learning, Alexandra Watral

Dissertations, Master's Theses and Master's Reports

To understand motor learning we must observe improvements in the performance of a motor behavior over time. Current laboratory approaches to measuring motor learning are not accessible to all populations, and this lack of accessibility limits the ability of researchers to gain information about developmental processes and medical conditions that impact motor control. To date, there are a handful of portable motor learning tools that use devices such as smartphones and tablets but very few fully remote options. We have created a web-based application to assess visuomotor adaptation, a gold standard approach to studying motor learning, in a remote setting. …

Investigating The Role Of Genomic Material Costs In Ecological, Evolutionary, And Invasion Dynamics Using The Solidago Gigantea (Giant Goldenrod) Polyploid Complex, Angela M. Walczyk

Dissertations, Master's Theses and Master's Reports

Polyploidy (whole genome duplication) is large-scale mutation that increases genome size and alters the genomic expression patterns, thus influencing morphological and physiological traits. Given that polyploids and progenitor diploids can be geographically segregated, and that polyploidy is prevalent in invasive plant taxa, polyploidy might affect the ability to tolerate and adapt to variable and/or novel environments. Responsiveness to soil nitrogen (N) and phosphorus (P) availability are of particular interest because 1) nucleic acids (DNA and RNA) require large amounts of N and P for their biosynthesis, potentially disadvantaging polyploids in nutrient-poor environments; and 2) biological invasions usually begin in urbanized …

Evaluation Of Production-Well Performance In Response To Formation Clogging And Rehabilitation For The Keweenaw Bay Tribal Fish Hatchery, Brock O. Howell

Dissertations, Master's Theses and Master's Reports

Temporally, the KBIC (Keweenaw Bay Indian Community) Hatchery wells have manifested general trends of declining specific capacity and higher energy consumption due to mineral precipitation in and around the production wells. Monitoring data, laboratory water analyses, and downhole video logging have confirmed that naturally occurring minerals are precipitating and clogging the screens and, likely, the surrounding formations. The reduction in fluid pressures in and around the well-screens from active pumping wells is typical but, ultimately alters the geochemical and physical conditions such that dissolved minerals precipitate on and around the well-screens, therefore, effectively clogging the well. The clogging from mineral …

Machine Learning-Driven Surrogate Models For Electrolytes, Tong Gao

Dissertations, Master's Theses and Master's Reports

We have developed a lattice Monte Carlo (MC) simulation based on the diffusion-limited aggregation model that accounts for the effect of the physical properties of ionic liquids (ILs) on lithium dendrite growth. Our simulations show that the size asymmetry between the cation and anion, the dielectric constant, and the volume fraction of ILs are critical factors to significantly suppress the dendrite growth, primarily due to substantial changes in electric-field screening. Specifically, the volume fraction of ILs has the optimal value for dendrite suppression. The present simulation method indicates potential challenges for the model extension to macroscopic systems. Therefore, we also …