Digital Commons Network^™

Jib-04 Has Broad-Spectrum Antiviral Activity And Inhibits Sars-Cov-2 Replication And Coronavirus Pathogenesis, Juhee Son, Shimeng Huang, Qiru Zeng, Traci L Bricker, James Brett Case, Ruochen Zang, Zhuoming Liu, Tamarand L Darling, Jian Xu, Houda H Harastani, Maria Florencia Gomez Castro, Hinissan P Kohio, Gaopeng Hou, Paul W Rothlauf, Adam L Bailey, Steven L Brody, Sean P J Whelan, Michael S Diamond, Adrianus C M Boon, Siyuan Ding, Et Al

Open Access Publications

Pathogenic coronaviruses are a major threat to global public health. Here, using a recombinant reporter virus-based compound screening approach, we identified small-molecule inhibitors that potently block the replication of severe acute respiratory syndrome virus 2 (SARS-CoV-2). Among them, JIB-04 inhibited SARS-CoV-2 replication in Vero E6 cells with a 50% effective concentration of 695 nM, with a specificity index of greater than 1,000. JIB-04 showed

Racial Discrimination In Life Insurance, William G. Gale, Kyle D. Logue, Nora Cahill, Rachel Gu, Swati Joshi

Law & Economics Working Papers

We examine the historical and statistical relationship between race and life insurance. Life insurance can play a central role in households’ financial security. Race has played an important and changing role in the provision of life insurance in the U.S. from slave insurance before the Civil War, to “Scientific Racism” continuing into the 20th century, to policies that do not explicitly mention race in recent decades. In empirical work using new data, we confirm earlier work showing that Black individuals have higher life insurance coverage rates than white individuals, controlling for observable characteristics. We find no difference in the likelihood …

Geospatial Applications Of Cave Resource Data To Better Understand Epikarst And Unsaturated Zone Groundwater Flow Path Development, Sierra M. Heimel, Benjamin W. Tobin

Faculty, Staff, and Affiliated Publications--KGS

The unsaturated zone is a critical component of karstic groundwater systems and is shown to provide substantial storage capacities. Understanding the spatial patterns and controls on flow path activation is often a challenge. Previous research focused on remotely sensed data or inferential analyses to quantify these patterns. Here, we use two cave systems—one in Arizona, USA and a second in Kentucky, USA—to show the value of the cave survey and inventory data in the direct observation of speleogenesis and unsaturated zone processes. Using geospatial statistical analyses, we show that passage size varies with distance from some faults, indicating that these …

The Earth Biogenome Project 2020: Starting The Clock, Harris A. Lewin, Stephen Richards, Erez Lieberman Aiden, Miguel L. Allende, John M. Archibald, Katharine B. Baker, Bridget Baumgartner, Katherine Belov, Giorgio Bertorelle, Mark L. Blaxter, Jing Cai, Nicolette D. Caperello, Keith Carlson, Juan Carlos Castilla-Rubio, Shu-Miaw Chaw, Lei Chen, Anna K. Childers, Jonathan A. Coddington, Dalia A. Conde, Montserrat Corominas, Keith A. Crandall, Andrew J. Crawford, Federica Dipalma, Richard Durbin, Thankgod E. Ebenezer, Scott V. Edwards, Olivier Fedrigo, Paul Flicek, Giulio Formenti, Richard A. Gibbs, M. Thomas P. Gilbert, Melissa M. Goldstein, Jennifer Marshall Graves, Henry T. Greely, Igor V. Grigoriev, Kevin J. Hackett, Neil Hall, David Haussler, Kristofer M. Helgen, Carolyn J. Hogg, Sachiko Isobe, Kjetill Sigurd Jakobson, Axel Janke, Erich D. Jarvis, Warren E. Johnson, Steven J. M. Jones, Elinor K. Karlsson, Paul J. Kersey, Jin-Hyoung Kim, W. John Kress, Shigehiro Kuraku, Mara K. N. Lawniczak, James H. Leebens-Mack, Xueyan Li, Kerstin Lindblad-Toh, Xin Liu, Jose V. Lopez, Tomas Marques-Bonet, Sophie Mazard, Jonna A. K. Mazet, Camila J. Mazzoni, Eugene W. Myers, Rachel J. O'Neill, Sadye Paez, Hyun Park, Gene E. Robinson, Cristina Roquet, Oliver A. Ryder, Jamal S. M. Sabir, H. Bradley Shaffer, Timothy M. Shank, Jacob S. Sherkow, Pamela S. Soltis, Boping Tang, Leho Tedersoo, Marcela Uliano-Silva, Kun Wang, Xiaofeng Wei, Regina Wetzer, Julia L. Wilson, Xun Xu, Huanming Yang, Anne D. Yoder, Guojie Zhang

Biology Faculty Articles

No abstract provided.

Standards Recommendations For The Earth Biogenome Project, Mara K. Lawniczak, Richard Durbin, Paul Flicek, Kerstin Lindblad-Toh, Xiaofeng Wei, John M. Archibald, William J. Baker, Katherine Belov, Mark L. Blaxter, Tomas Marques-Bonet, Anna K. Childers, Jonathan A. Coddington, Keith A. Crandall, Andrew J. Crawford, Robert P. Davey, Federica Di Palma, Qi Fang, Wilfried Haerty, Neil Hall, Katherine J. Hoff, Kerstin Howe, Erich D. Jarvis, Warren E. Johnson, Rebecca N. Johnson, Paul J. Kersey, Xin Liu, Jose V. Lopez, Eugene W. Myers, Olga Vinnere Pettersson, Adam M. Phillippy, Monica F. Poelchau, Kim D. Pruitt, Arang Rhie, Juan Carlos Castilla-Rubio, Sunil Kumar Sahu, Nicholas A. Salmon, Pamela S. Soltis, David Swarbreck, Françoise Thibaud-Nissen, Sibo Wang, Jill L. Wegrzyn, Guojie Zhang, He Zhang, Harris A. Lewin, Stephen Richards

Biology Faculty Articles

A global international initiative, such as the Earth BioGenome Project (EBP), requires both agreement and coordination on standards to ensure that the collective effort generates rapid progress toward its goals. To this end, the EBP initiated five technical standards committees comprising volunteer members from the global genomics scientific community: Sample Collection and Processing, Sequencing and Assembly, Annotation, Analysis, and IT and Informatics. The current versions of the resulting standards documents are available on the EBP website, with the recognition that opportunities, technologies, and challenges may improve or change in the future, requiring flexibility for the EBP to meet its goals. …

Why Sequence All Eukaryotes?, Mark Blaxter, John M. Archibald, Anna K. Childers, Jonathan A. Coddington, Keith A. Crandall, Federica Di Palma, Richard Durbin, Scott V. Edwards, Jennifer A.M. Graves, Kevin J. Hackett, Neil Hall, Erich D. Jarvis, Rebecca N. Johnson, Elinor K. Karlsson, W. John Kress, Shigehiro Kuraku, Mara K. N. Lawniczak, Kerstin Lindblad-Toh, Jose V. Lopez, Nancy A. Moran, Gene E. Robinson, Oliver A. Ryder, Beth Shapiro, Pamela S. Soltis, Tandy Warnow, Guojie Zhang, Harris A. Lewin

Biology Faculty Articles

Life on Earth has evolved from initial simplicity to the astounding complexity we experience today. Bacteria and archaea have largely excelled in metabolic diversification, but eukaryotes additionally display abundant morphological innovation. How have these innovations come about and what constraints are there on the origins of novelty and the continuing maintenance of biodiversity on Earth? The history of life and the code for the working parts of cells and systems are written in the genome. The Earth BioGenome Project has proposed that the genomes of all extant, named eukaryotes—about 2 million species—should be sequenced to high quality to produce a …

Ethical, Legal, And Social Issues In The Earth Biogenome Project, Jacob S. Sherkow, Katharine B. Barker, Irus Braverman, Robert Cook-Deegan, Richard Durbin, Carla L. Easter, Melissa M. Goldstein, Maui Hudson, W. John Kress, Harris A. Lewin, Debra J. H. Mathews, Catherine Mccarthy, Ann M. Mccartney, Manuela Da Silva, Andrew W. Torrance, Henry T. Greely

Journal Articles

The Earth BioGenome Project (EBP) is an audacious endeavor to obtain whole-genome sequences of representatives from all eukaryotic species on Earth. In addition to the project’s technical and organizational challenges, it also faces complicated ethical, legal, and social issues. This paper, from members of the EBP’s Ethical, Legal, and Social Issues (ELSI) Committee, catalogs these ELSI concerns arising from EBP. These include legal issues, such as sample collection and permitting; the applicability of international treaties, such as the Convention on Biological Diversity and the Nagoya Protocol; intellectual property; sample accessioning; and biosecurity and ethical issues, such as sampling from the …

Ucc Minutes January 18, 2022, Angela Brock

UCC Agendas/Minutes

No abstract provided.

Icc Agenda 1-18-22, Angela Brock

Individual Course Committee (ICC)

No abstract provided.

Approved Courses January 18, 2022, Angela Brock

Individual Course Committee (ICC)

No abstract provided.

The Biological Activity Of Mycosynthesized Silver Nanoparticles Against Some Pathogenic Bacteria, Rana Hikmet, Nehia Hussein

Journal of Applied Sciences and Nanotechnology

This study was carried out for the mycosynthesis of silver nanoparticles by Candida albicans supernatant.  All the isolates used in this study were taken from the patients who existed at Al-Elweya children's teaching hospital in Baghdad, Iraq. Mycosynthesized silver nanoparticles were characterized by color visualization, ultraviolet-visible (UV) spectroscopy, and Fourier transform infrared spectroscopy (FTIR). The UV-Vis spectroscopy examination has shown the highest absorbance (λmax) at the wavelength of 429 nanometers, which indicated the creation of silver nanoparticles. Furthermore, the results of the antibacterial potential of AgNO3 and AgNPs against Klebsiella pneumoniae and Staphylococcus haemolyticus bacteria showed the highest effect of …

Which Exercise Interventions Can Most Effectively Improve Reactive Balance In Older Adults? A Systematic Review And Network Meta-Analysis, Youngwook Kim, Michael N. Vakula, David A. E. Bolton, Christopher J. Dakin, Brennan J. Thompson, Timothy A. Slocum, Masaru Teramoto, Eadric Bressel

Kinesiology and Health Science Faculty Publications

Background: Reactive balance is the last line of defense to prevent a fall when the body loses stability, and beneficial effects of various exercise-based interventions on reactive balance in older adults have been reported. However, their pooled evidence on the relative effects has yet to be described. Objective: To review and evaluate the comparative effectiveness of various exercise-based interventions on reactive balance in older adults. Methods: Nine electronic databases and reference lists were searched from inception to August 2021. Eligibility criteria according to PICOS criteria were as follows: (1) population: older adults with the mean age of 65 years or …

Spotlight On Nociceptin/Orphanin Fq Receptor In The Treatment Of Pain, Amal El Daibani, Tao Che

Open Access Publications

No abstract provided.

Reevaluation Of The Role Of Lip-1 As An Erk/Mpk-1 Dual Specificity Phosphatase In The C. Elegans Germline, Debabrata Das, Jacob Seemann, David Greenstein, Tim Schedl, Swathi Arur

Open Access Publications

The fidelity of a signaling pathway depends on its tight regulation in space and time. Extracellular signal-regulated kinase (ERK) controls wide-ranging cellular processes to promote organismal development and tissue homeostasis. ERK activation depends on a reversible dual phosphorylation on the TEY motif in its active site by ERK kinase (MEK) and dephosphorylation by DUSPs (dual specificity phosphatases). LIP-1, a DUSP6/7 homolog, was proposed to function as an ERK (MPK-1) DUSP in the

Comparative Genomics Of Borderline Oxacillin-Resistant Staphylococcus Aureus Detected During A Pseudo-Outbreak Of Methicillin-Resistant S. Aureus In A Neonatal Intensive Care Unit, Sanjam S Sawhney, Eric M Ransom, Meghan A Wallace, Patrick J Reich, Gautam Dantas, Carey-Ann D Burnham

Open Access Publications

Active surveillance for methicillin-resistant Staphylococcus aureus (MRSA) is a component of our neonatal intensive care unit (NICU) infection prevention efforts. Recent atypical trends prompted review of 42 suspected MRSA isolates. Species identification was confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), and methicillin resistance was reevaluated by PBP2a lateral flow assay, cefoxitin/oxacillin susceptibility testing,

B Cell Receptor Isotypes Differentially Associate With Cell Signaling, Kinetics, And Outcome In Chronic Lymphocytic Leukemia, Andrea N Mazzarello, Eva Gentner-Göbel, Marcus Dühren-Von Minden, Tatyana N Tarasenko, Antonella Nicolò, Gerardo Ferrer, Stefano Vergani, Yun Liu, Davide Bagnara, Kanti R Rai, Jan A Burger, Peter J Mcguire, Palash C Maity, Hassan Jumaa, Nicholas Chiorazzi

Faculty, Staff and Student Publications

In chronic lymphocytic leukemia (CLL), the B cell receptor (BCR) plays a critical role in disease development and progression, as indicated by the therapeutic efficacy of drugs blocking BCR signaling. However, the mechanism(s) underlying BCR responsiveness are not completely defined. Selective engagement of membrane IgM or IgD on CLL cells, each coexpressed by more than 90% of cases, leads to distinct signaling events. Since both IgM and IgD carry the same antigen-binding domains, the divergent actions of the receptors are attributed to differences in immunoglobulin (Ig) structure or the outcome of signal transduction. We showed that IgM, not IgD, level …

Becoming With Pigs, Plants, And People: Examining The Formation Of Human-Animal Mutualisms In Neolithic Northeastern China, Ximena Lemoine

Arts & Sciences Graduate Student Theses and Dissertations

More than 10,000 years ago humans in multiple parts of Eurasia began to form increasingly more entangled and mutually dependent relationships with non-human animals. In some cases, the elaboration of these relationships would result in evolutionary change, resulting in biologically distinct or domesticated populations. The last several decades of research show that this process is ongoing and polymorphous, emerging out of sustained human-animal interactions that develop along historically contingent trajectories shaped by particular biological, environmental, and cultural contexts. Thus, understanding domestication as an evolutionary process necessitates investigation into species- and context-specific developmental trajectories of human-animal interaction. The pig (Sus scrofa) …

Characterizing Human Gut Bacterial Tir Domains In Vitro, In Gnotobiotic Mice And Malnourished Children, James Stephen Weagley

Arts & Sciences Graduate Student Theses and Dissertations

Worldwide, malnutrition (undernutrition) is the leading cause of death in children under 5. Studies of healthy members of birth cohorts from low- and middle-income countries have identified a program of gut microbial community development that is disrupted in children with severe acute malnutrition (SAM) and moderate acute malnutrition (MAM). This perturbed gut microbiome development has been linked to the pathogenesis of malnutrition. Repair of this gut microbial dysbiosis in children with MAM with microbiota-directed complementary foods (MDCFs) designed to increase the fitness and expressed beneficial functions of underrepresented bacterial community members restores healthy growth, including improvements in mediators of metabolic …

Phage-Encoded Cationic Antimicrobial Peptide Required For Lysis, Ashley Holt, Jesse Cahill, Jolene Ramsey, Cody Martin, Chandler O’Leary, Russell Moreland Iii, Lori T. Maddox, Thushara Galbadage, Riti Sharan, Preeti Sule

School of Medicine Publications

Abstract: Most phages of Gram-negative bacteria hosts encode spanins for disruption of the outer membrane, which is the last step in host lysis. However, bioinformatic analysis indicates that ∼15% of these phages lack a spanin gene, suggesting they have an alternate way of disrupting the outer membrane (OM). Here, we show that the T7-like coliphage phiKT causes an explosive cell lysis associated with spanin activity despite not encoding spanins. A putative lysis cassette cloned from the phiKT late gene region includes the hypothetical novel gene 28 located between the holin and endolysin genes and supports inducible lysis in Escherichia …

Rspo2 And Rankl Signal Through Lgr4 To Regulate Osteoclastic Premetastatic Niche Formation And Bone Metastasis, Zhiying Yue, Xin Niu, Zengjin Yuan, Qin Qin, Wenhao Jiang, Liang He, Jingduo Gao, Yi Ding, Yanxi Liu, Ziwei Xu, Zhenxi Li, Zhengfeng Yang, Rong Li, Xiwen Xue, Yankun Gao, Fei Yue, Xiang H-F Zhang, Guohong Hu, Yi Wang, Yi Li, Geng Chen, Stefan Siwko, Alison Gartland, Ning Wang, Jianru Xiao, Mingyao Liu, Jian Luo

Faculty, Staff and Students Publications

Therapeutics targeting osteoclasts are commonly used treatments for bone metastasis; however, whether and how osteoclasts regulate premetastatic niche and bone tropism are largely unknown. In this study, we report that osteoclast precursors (OPs) can function as a premetastatic niche component that facilitates breast cancer (BCa) bone metastasis at early stages. At the molecular level, unbiased GPCR ligand/agonist screening in BCa cells suggested that R-spondin 2 (RSPO2) and RANKL, through interaction with their receptor LGR4, promoted osteoclastic premetastatic niche formation and enhanced BCa bone metastasis. This was achieved by RSPO2/RANKL-LGR4 signal modulating the WNT inhibitor DKK1 through Gαq and β-catenin signaling. …

Effect Of P2y12 Inhibitors On Survival Free Of Organ Support Among Non-Critically Ill Hospitalized Patients With Covid-19: A Randomized Clinical Trial, Jeffrey S Berger, Lucy Z Kornblith, Michelle N Gong, Harmony R Reynolds, Mary Cushman, Yu Cheng, Bryan J Mcverry, Keri S Kim, Renato D Lopes, Bassel Atassi, Scott Berry, Grant Bochicchio, Murillo De Oliveira Antunes, Michael E Farkouh, Yonatan Greenstein, Erinn M Hade, Kristin Hudock, Robert Hyzy, Pooja Khatri, Andrei Kindzelski, Bridget-Anne Kirwan, Lisa Baumann Kreuziger, Patrick R Lawler, Eric Leifer, Jose Lopez-Sendon Moreno, Jose Lopez-Sendon, James F Luther, Lilia Nigro Maia, John Quigley, Robert Sherwin, Lana Wahid, Jennifer Wilson, Judith S Hochman, Matthew D Neal, Activ-4a Investigators

Faculty, Staff and Students Publications

IMPORTANCE: Platelets represent a potential therapeutic target for improved clinical outcomes in patients with COVID-19.

OBJECTIVE: To evaluate the benefits and risks of adding a P2Y12 inhibitor to anticoagulant therapy among non-critically ill patients hospitalized for COVID-19.

DESIGN, SETTING, AND PARTICIPANTS: An open-label, bayesian, adaptive randomized clinical trial including 562 non-critically ill patients hospitalized for COVID-19 was conducted between February 2021 and June 2021 at 60 hospitals in Brazil, Italy, Spain, and the US. The date of final 90-day follow-up was September 15, 2021.

INTERVENTIONS: Patients were randomized to a therapeutic dose of heparin plus a P2Y12 inhibitor (n = …

Changes In Crude Protein And Fiber Contents Of Small Grain Cereals For Forage Over Time, J. L. Zaragoza-Ramirez, N. Zayago-Ramos, P. A. Martinez-Hernandez

IGC Proceedings (1977-2023)

Oat, triticale, wheat and barley are small grain cereals used as forage in many temperate Mexican regions. The objective was to determine crude protein and van Soest fiber contents of these forages cut at 80, 96, 108, 121, 138 and 153 days after seeding. Cultivars used were: Chihuahua (OC) for oat; Arne (TA), Bicentenario (TB) and Siglo XXI (TS) for triticale; Saturno (WS) for wheat; and San Marcos (BSM) for barley. Experiment was under greenhouse conditions from November 2015 to May 2016. Crude protein (CP), neutral (NDF) and acid (ADF) detergents fibers were determined on the forage harvested at each …

Collection Priorities Of Pasture Genetic Resources In The Paniceae Native To South Africa, F. L. Müller, M. Trytsman, E. L. Masemola, M. I. Kgonothi, N. R. Mkhize, T. J. Tjelele

IGC Proceedings (1977-2023)

There is an urgent need for the prioritization and ex situ conservation of pasture grass species. Many pasture grasses are still in the process of domestication and grasslands in the tropical, subtropical, semi-arid and arid regions of the world are therefore a major source of genetic material for future forage improvement. Within South Africa, it was found that the Paniceae contains several species with high pasture value and that many populations of these species are found in water-limited environments. Therefore, it was proposed that a strategy be developed for the South African National Forage Genebank to prioritize efforts to collect …

Changes In Defoliation Patterns Of Plant Functional Groups Under Variable Herbage Allowance In Campos Grasslands, N. Caram, F. Casalás, Pablo Soca, M. Wallau, M. Cadenazzi, P. Boggiano

IGC Proceedings (1977-2023)

Several studies have evaluated separately forage production, botanical composition, leaf traits and animal performance. However, few of them have focused on defoliation patterns at the level of functional groups (FGs) under different and variable herbage allowance (HA), especially in natural, diverse grasslands. The objective was to evaluate the relationship between HA and leaf traits on defoliation patterns of mature beef cows in the autumn, winter and spring. We evaluated the grazing probability (GP), intensity of defoliation (ID), and leaf traits on 14 species that represent more than 80% of total dry matter of the pasture. The experiment at which we …

Examining Alternative Water Management Strategies To Support Rio Grande Silvery Minnow Conservation Within And Across Years, Timothy E. Walsworth, Phaedra Budy

Watershed Sciences Faculty Publications

Rio Grande Silvery Minnow (RGSM) are currently constrained to only 5% of their historic range, and their persistence is threatened by highly altered and impaired habitat conditions (Bestgen and Platania 1991). These habitat conditions have resulted from reduced spring and summer flows due to natural variability and anthropogenic water development and extraction, which have resulted in substantial geomorphic changes (Swanson et al. 2011). Successful conservation of this endangered species will require determination of how available flows can be managed to provide conditions supporting growth, reproduction, and survival of RGSM within and across a variety of water years. Previous research has …

The Utah Statesman, January 17, 2022, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

“Part Of Something Larger Than Myself”: Lessons Learned From A Multidisciplinary, Multicultural, And Multilingual International Research Team Of Academic Women, Kristina S. Brown, Tricia M. Farwell, Sara Bender, Alpha A. Martinez-Suarez, Stefani Boutelier, Agata A. Lambrechts, Iwona Leonowicz-Bukała, Pipiet Larasatie

All Faculty Scholarship for the College of the Sciences

Bringing our collective experiences of past collaborations through a virtual connection, we created an international research team of 16 multidiscipline, multicultural, and multilingual academic women called “COVID GAP” (Gendered Academic Productivity) to explore the ongoing challenges and effects of COVID-19. Identifying as insider researchers, we engaged in a two-phase, primarily qualitative research project to better understand the lived experiences of academics during the pandemic. Our past individual experiences with cooperative research informed our roles and responsibilities and how we organized and communicated. This article is a reflection of how COVID GAP has refined our collaborative process in response to an …

Ultrasound 96 Probe Device Protocol For Cancer Cell Treatment, Aisling Field, Brijesh K. Tiwari, James F. Curtin, Julie R M Mondala, Janith Wanigasekara

Articles

Ultrasound is a sound wave with frequencies ranging between 20 kHz and 20 MHz. Ultrasound is able to temporarily and repeatedly open the BBB safely and enhance chemotherapeutic delivery without adverse effects. This novel technique in drug delivery benefits from the powerful ability of ultrasound to produce cavitation activity. Cavitation is the generation and activity of gas-filled bubbles in a medium exposed to ultrasound. As the pressure wave passes through the media, gas bubbles expand at low pressure and contract at high pressure. This leads to oscillation which produces a circulating fluid flow known as microstreaming around the bubble with …

Detoxification Of Copper And Chromium Via Dark Hydrogen Fermentation Of Potato Waste By Clostridium Butyricum Strain 92, Vira Hovorukha, Olesia Havryliuk, Galina Gladka, Antonina Kalinichenko, Monika Sporek, Jan Stebila, Dmitri Mavrodi, Ruslan Mariychuk, Oleksandr Tashyrev

Faculty Publications

The accumulation of various types of waste containing both organic and inorganic metal-containing compounds is extremely hazardous for living organisms. The possibility of polymer degradation, biohydrogen synthesis, and metal detoxification via the dark fermentation of model potato waste was investigated. For this purpose, the strict anaerobic strain was isolated and identified as Clostridium butyricum. The high efficiency of dark hydrogen fermentation of potatoes with yield of hydrogen in 85.8 ± 15.3 L kg⁻¹ VS_potato was observed. The copperand chromium salts solutions were added to the culture fluid to obtain the concentrations of 50, 100, and 200 mg …

Crow's Nest, January 17, 2022, Crow's Nest Staff

Crow's Nest

The January 17, 2022, issue of the Crow's Nest.