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2022

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Articles 22831 - 22860 of 32057

Full-Text Articles in Entire DC Network

Chancellor's Cabinet Updates: 2022-01-25, Karen Carey, Maren Haavig, Kristen Handley, Michael Ciri, Pete Traxler, Lori Klein, Jackie Wilson, Carin Silkaitis, Ronalda Cadiente Brown, Keni Campbell, Lynne Johnson, Elise Tomlinson, Priscilla Schulte, Paul Kraft, Nathan Leigh Jan 2022

Chancellor's Cabinet Updates: 2022-01-25, Karen Carey, Maren Haavig, Kristen Handley, Michael Ciri, Pete Traxler, Lori Klein, Jackie Wilson, Carin Silkaitis, Ronalda Cadiente Brown, Keni Campbell, Lynne Johnson, Elise Tomlinson, Priscilla Schulte, Paul Kraft, Nathan Leigh

Chancellor's Cabinet Updates

No abstract provided.


How Capuchin Monkeys Use Their Semi-Prehensile Tails, Madhur Mangalam, Callum F. Ross, Patricia Izar, Elisabetta Visalberghi, Dorothy M. Fragaszy Jan 2022

How Capuchin Monkeys Use Their Semi-Prehensile Tails, Madhur Mangalam, Callum F. Ross, Patricia Izar, Elisabetta Visalberghi, Dorothy M. Fragaszy

Journal Articles

Among primates, prehensile/semi-prehensile tails have evolved independently in the families Atelidae and Cebidae of the infraorder Platyrrhini (Neotropical monkeys). They facilitate maintaining stability during locomotion on thin, flexible branches and while reaching for food on challenging substrates. How a prehensile/semi-prehensile tail is coordinated with the hind limbs to facilitate controlled, flexible adoption of postures remains unknown. In an experimental set-up in the wild, we induced capuchin monkeys to adopt a tail-assisted, head-down tripodal posture to reach for food, documenting from slow-motion video recordings (120 fps) both qualitative changes in the monkeys’ positional behaviour – the relative orientation of their limbs …


Life In The Lab, Christina Casinelli Jan 2022

Life In The Lab, Christina Casinelli

UNH Today Archive

No abstract provided.


Tisagenlecleucel Outcomes In Relapsed/Refractory Extramedullary All: A Pediatric Real World Car Consortium Report., Vanessa A. Fabrizio, Christine L. Phillips, Adam Lane, Christina Baggott, Snehit Prabhu, Emily Egeler, Sharon Mavroukakis, Holly L. Pacenta, Jenna Rossoff, Heather Stefanski, Julie-An An Talano, Amy Moskop, Steven P. Margossian, Michael R. Verneris, Douglas Myers, Nicole A. Karras, Patrick A. Brown, Muna Qayed, Michelle L. Hermiston, Prakash Satwani, Christa Krupski, Amy K. Keating, Rachel Wilcox, Cara A. Rabik, Vasant Chinnabhandar, Michael Kunicki, A Yasemin Goksenin, Kevin J. Curran, Crystal L. Mackall, Theodore W. Laetsch, Liora M. Schultz Jan 2022

Tisagenlecleucel Outcomes In Relapsed/Refractory Extramedullary All: A Pediatric Real World Car Consortium Report., Vanessa A. Fabrizio, Christine L. Phillips, Adam Lane, Christina Baggott, Snehit Prabhu, Emily Egeler, Sharon Mavroukakis, Holly L. Pacenta, Jenna Rossoff, Heather Stefanski, Julie-An An Talano, Amy Moskop, Steven P. Margossian, Michael R. Verneris, Douglas Myers, Nicole A. Karras, Patrick A. Brown, Muna Qayed, Michelle L. Hermiston, Prakash Satwani, Christa Krupski, Amy K. Keating, Rachel Wilcox, Cara A. Rabik, Vasant Chinnabhandar, Michael Kunicki, A Yasemin Goksenin, Kevin J. Curran, Crystal L. Mackall, Theodore W. Laetsch, Liora M. Schultz

Manuscripts, Articles, Book Chapters and Other Papers

Chimeric antigen receptor (CAR) T cells have transformed the therapeutic options for relapsed/refractory (R/R) B-cell acute lymphoblastic leukemia. Data for CAR therapy in extramedullary (EM) involvement are limited. Retrospective data were abstracted from the Pediatric Real World CAR Consortium (PRWCC) of 184 infused patients from 15 US institutions. Response (complete response) rate, overall survival (OS), relapse-free survival (RFS), and duration of B-cell aplasia (BCA) in patients referred for tisagenlecleucel with EM disease (both central nervous system (CNS)3 and non-CNS EM) were compared with bone marrow (BM) only. Patients with CNS disease were further stratified for comparison. Outcomes are reported on …


Eric Monat, Oral History Interview, 2022, Matt Jones Jan 2022

Eric Monat, Oral History Interview, 2022, Matt Jones

Oral Histories

In 2021, Eastern Michigan University Archives lecturer Matt Jones began documenting the story of Ypsilanti’s Human Rights Ordinance #1279 in an effort to explore the ways in which local queer activism has evolved multi-generationally in Ypsilanti. What began as a refusal of service by a local print shop to a small EMU student group quickly turned into a years-long battle over who was deserving of basic human rights. To the LGBTQ activists and community members documented here, they had always been present in the community: working, paying taxes, painting their houses, mowing their lawns, attending council meetings, and even serving …


Machine Learning For Predicting Risk Of Early Dropout In A Recovery Program For Opioid Use Disorder, Assaf Gottlieb, Andrea Yatsco, Christine Bakos-Block, James R Langabeer, Tiffany Champagne-Langabeer Jan 2022

Machine Learning For Predicting Risk Of Early Dropout In A Recovery Program For Opioid Use Disorder, Assaf Gottlieb, Andrea Yatsco, Christine Bakos-Block, James R Langabeer, Tiffany Champagne-Langabeer

Faculty, Staff and Student Publications

BACKGROUND: An increase in opioid use has led to an opioid crisis during the last decade, leading to declarations of a public health emergency. In response to this call, the Houston Emergency Opioid Engagement System (HEROES) was established and created an emergency access pathway into long-term recovery for individuals with an opioid use disorder. A major contributor to the success of the program is retention of the enrolled individuals in the program.

METHODS: We have identified an increase in dropout from the program after 90 and 120 days. Based on more than 700 program participants, we developed a machine learning …


The Importance Of Abolition Of The Carceral State For Native Survivors, Christina M. Schnalzer Jan 2022

The Importance Of Abolition Of The Carceral State For Native Survivors, Christina M. Schnalzer

American Indian Law Journal

No abstract provided.


Evaluation Of Bioactive Properties Of Lipophilic Fractions Of Edible And Non-Edible Parts Of Nasturtium Officinale (Watercress) In A Model Of Human Malignant Melanoma Cells, Sotiris Kyriakou, Venetia Tragkola, Heba Alghol, Ioannis Anestopoulos, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis Jan 2022

Evaluation Of Bioactive Properties Of Lipophilic Fractions Of Edible And Non-Edible Parts Of Nasturtium Officinale (Watercress) In A Model Of Human Malignant Melanoma Cells, Sotiris Kyriakou, Venetia Tragkola, Heba Alghol, Ioannis Anestopoulos, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Watercress is an enriched source of phenethyl isothiocyanate (PEITC), among other phytochemicals, with an antioxidant capacity. The aim of this study was to (i) chemically characterize and (ii) biologically evaluate the profile of the main health-promoting compounds contained in edible (i.e., mixture of leaves and lateral buds) and non-edible (i.e., stems) parts of watercress in an in vitro model of malignant melanoma consisting of human malignant melanoma (A375), non-melanoma (A431) and keratinocyte (HaCaT) cells. The extraction of the main constituents of watercress was performed by subjecting the freeze-dried edible and non-edible samples through different extraction protocols, whereas their concentration was …


Utility Of Blood As The Clinical Specimen For The Diagnosis Of Ocular Toxoplasmosis Using Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification And Real-Time Polymerase Chain Reaction Assays Based On Rep-529 Sequence And B1 Gene, Bahman Rahimi Esboei, Shirzad Fallahi, Mohammad Zarei, Bahram Kazemi, Mehdi Mohebali, Saeedeh Shojaee, Parisa Mousavi, Aref Teimouri, Raziyeh Mahmoudzadeh, Mirataollah Salabati, Hossein Keshavarz Valian Jan 2022

Utility Of Blood As The Clinical Specimen For The Diagnosis Of Ocular Toxoplasmosis Using Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification And Real-Time Polymerase Chain Reaction Assays Based On Rep-529 Sequence And B1 Gene, Bahman Rahimi Esboei, Shirzad Fallahi, Mohammad Zarei, Bahram Kazemi, Mehdi Mohebali, Saeedeh Shojaee, Parisa Mousavi, Aref Teimouri, Raziyeh Mahmoudzadeh, Mirataollah Salabati, Hossein Keshavarz Valian

Wills Eye Hospital Papers

Background: Ocular infection with Toxoplasma gondii is a major preventable cause of blindness, especially in young people. The aim of the present study was to assess detection rate of T. gondii DNA in blood samples of clinically diagnosed of ocular toxoplasmosis using uracil DNA glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) and real-time quantitative PCR (qPCR) based on REP-529 and B1.

Methods: One hundred and seventeen patients with clinically diagnosed ocular toxoplasmosis (OT) were participated in the study as well as 200 control patients. Peripheral blood samples were assessed using UDG-LAMP and qPCR techniques targeting REP-529 and B1.

Results: Detection limits of …


Morphological Phenotypes, Cell Division, And Gene Expression Of Escherichia Coli Under High Concentration Of Sodium Sulfate, Khanh Nguyen, Pradeep Kumar Jan 2022

Morphological Phenotypes, Cell Division, And Gene Expression Of Escherichia Coli Under High Concentration Of Sodium Sulfate, Khanh Nguyen, Pradeep Kumar

Physics Faculty Publications and Presentations

Sodium and sulfate ions are among the suggested abundant ions on Europa, a moon of Jupiter. In order to investigate the potential habitability of Europa, we study the effects of sodium sulfate (Na2SO4) on a non-halophilic bacterium by subjecting Escherichia coli (E. coli) to a wide range of Na2SO4 concentrations (0–1.0 m). We discover that, as the concentration of sodium sulfate increases, the biomass doubling time increases and the cell growth is completely inhibited at 1.0" role="presentation" style="box-sizing: border-box; max-height: none; display: inline; line-height: normal; font-size: 13.2px; text-align: left; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: …


Nischarin Deletion Reduces Oxidative Metabolism And Overall Atp: A Study Using A Novel NischΔ5‐6 Knockout Mouse Model, Tina H. Nguyen, Hassan Yousefi, Samuel C. Okpechi, Lothar Lauterboeck, Shengli Dong, Qinglin Yang, Suresh K. Alahari Jan 2022

Nischarin Deletion Reduces Oxidative Metabolism And Overall Atp: A Study Using A Novel NischΔ5‐6 Knockout Mouse Model, Tina H. Nguyen, Hassan Yousefi, Samuel C. Okpechi, Lothar Lauterboeck, Shengli Dong, Qinglin Yang, Suresh K. Alahari

School of Medicine Faculty Publications

Nischarin (Nisch) is a cytosolic scaffolding protein that harbors tumor‐suppressor‐like characteristics. Previous studies have shown that Nisch functions as a scaffolding protein and regulates multiple biological activities. In the current study, we prepared a complete Nisch knockout model, for the first time, by deletion of exons 5 and 6. This knockout model was confirmed by Qrt– PCR and Western blotting with products from mouse embryonic fibroblast (MEF) cells. Embryos and adult mice of knockouts are significantly smaller than their wild‐type counterparts. Deletion of Nisch enhanced cell migration, as demonstrated by wound type and transwell migration assays. Since the animals were …


Preconception Maternal Posttraumatic Stress And Child Negative Affectivity: Prospectively Evaluating The Intergenerational Impact Of Trauma, Danielle A. Swales, Elysia Poggi Davis, Nicole E. Mahrer, Christine M. Guardino, Madeleine Shalowitz, Sharon L. Ramey, Christine Dunkel Schetter Jan 2022

Preconception Maternal Posttraumatic Stress And Child Negative Affectivity: Prospectively Evaluating The Intergenerational Impact Of Trauma, Danielle A. Swales, Elysia Poggi Davis, Nicole E. Mahrer, Christine M. Guardino, Madeleine Shalowitz, Sharon L. Ramey, Christine Dunkel Schetter

Psychology: Faculty Scholarship

The developmental origins of psychopathology begin before birth and perhaps even prior to conception. Understanding the intergenerational transmission of psychopathological risk is critical to identify sensitive windows for prevention and early intervention. Prior research demonstrates that maternal trauma history, typically assessed retrospectively, has adverse consequences for child socioemotional development. However, very few prospective studies of preconception trauma exist, and the role of preconception symptoms of posttraumatic stress disorder (PTSD) remains unknown. The current study prospectively evaluates whether maternal preconception PTSD symptoms predict early childhood negative affectivity, a key dimension of temperament and predictor of later psychopathology. One hundred and eighteen …


Healthcare Self-Governance, Danika Watson Jan 2022

Healthcare Self-Governance, Danika Watson

American Indian Law Journal

No abstract provided.


Whole-Exome Analysis Of Adolescents With Low Vwf And Heavy Menstrual Bleeding Identifies Novel Genetic Associations, Brooke Sadler, Christina A Gurnett, Jorge Di Paola, Et Al Jan 2022

Whole-Exome Analysis Of Adolescents With Low Vwf And Heavy Menstrual Bleeding Identifies Novel Genetic Associations, Brooke Sadler, Christina A Gurnett, Jorge Di Paola, Et Al

Open Access Publications

Adolescents with low von Willebrand factor (VWF) levels and heavy menstrual bleeding (HMB) experience significant morbidity. There is a need to better characterize these patients genetically and improve our understanding of the pathophysiology of bleeding. We performed whole-exome sequencing on 86 postmenarchal patients diagnosed with low VWF levels (30-50 IU/dL) and HMB and compared them with 660 in-house controls. We compared the number of rare stop-gain/stop-loss and rare ClinVar "pathogenic" variants between cases and controls, as well as performed gene burden and gene-set burden analyses. We found an enrichment in cases of rare stop-gain/stop-loss variants in genes involved in bleeding …


Longitudinal Study After Sputnik V Vaccination Shows Durable Sars-Cov-2 Neutralizing Antibodies And Reduced Viral Variant Escape To Neutralization Over Time, María M Gonzalez Lopez Ledesma, Paul W Rothlauf, Zhuoming Liu, Louis-Marie Bloyet, Marjorie Cornejo Pontelli, Et Al Jan 2022

Longitudinal Study After Sputnik V Vaccination Shows Durable Sars-Cov-2 Neutralizing Antibodies And Reduced Viral Variant Escape To Neutralization Over Time, María M Gonzalez Lopez Ledesma, Paul W Rothlauf, Zhuoming Liu, Louis-Marie Bloyet, Marjorie Cornejo Pontelli, Et Al

Open Access Publications

Recent studies have shown a temporal increase in the neutralizing antibody potency and breadth to SARS-CoV-2 variants in coronavirus disease 2019 (COVID-19) convalescent individuals. Here, we examined longitudinal antibody responses and viral neutralizing capacity to the B.1 lineage virus (Wuhan related), to variants of concern (VOC; Alpha, Beta, Gamma, and Delta), and to a local variant of interest (VOI; Lambda) in volunteers receiving the Sputnik V vaccine in Argentina. Longitudinal serum samples (


Systematic Decomposition Of Sequence Determinants Governing Crispr/Cas9 Specificity, Rongjie Fu, Wei He, Jinzhuang Dou, Oscar D Villarreal, Ella Bedford, Helen Wang, Connie Hou, Liang Zhang, Yalong Wang, Dacheng Ma, Yiwen Chen, Xue Gao, Martin Depken, Han Xu Jan 2022

Systematic Decomposition Of Sequence Determinants Governing Crispr/Cas9 Specificity, Rongjie Fu, Wei He, Jinzhuang Dou, Oscar D Villarreal, Ella Bedford, Helen Wang, Connie Hou, Liang Zhang, Yalong Wang, Dacheng Ma, Yiwen Chen, Xue Gao, Martin Depken, Han Xu

Faculty, Staff and Student Publications

The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (gRNA) and the targeted DNA, yet the sequence-dependent rules underlying off-target effects are not fully understood. To systematically explore the sequence determinants governing CRISPR/Cas9 specificity, here we describe a dual-target system to measure the relative cleavage rate between off- and on-target sequences (off-on ratios) of 1902 gRNAs on 13,314 synthetic target sequences, and reveal a set of sequence rules involving 2 factors in off-targeting: 1) a guide-intrinsic mismatch tolerance (GMT) independent of the mismatch context; 2) an "epistasis-like" combinatorial effect of multiple mismatches, which are …


Inhibiting Mycobacterium Abscessus Cell Wall Synthesis: Using A Novel Diazabicyclooctane Β-Lactamase Inhibitor To Augment Β-Lactam Action, Khalid M Dousa, David C Nguyen, Sebastian G Kurz, Magdalena A Taracila, Christopher R Bethel, William Schinabeck, Barry N Kreiswirth, Sheldon T Brown, W Henry Boom, Richard S Hotchkiss, Kenneth E Remy, Frank J Jacono, Charles L Daley, Steven M Holland, Alita A Miller, Robert A Bonomo Jan 2022

Inhibiting Mycobacterium Abscessus Cell Wall Synthesis: Using A Novel Diazabicyclooctane Β-Lactamase Inhibitor To Augment Β-Lactam Action, Khalid M Dousa, David C Nguyen, Sebastian G Kurz, Magdalena A Taracila, Christopher R Bethel, William Schinabeck, Barry N Kreiswirth, Sheldon T Brown, W Henry Boom, Richard S Hotchkiss, Kenneth E Remy, Frank J Jacono, Charles L Daley, Steven M Holland, Alita A Miller, Robert A Bonomo

Open Access Publications

Mycobacterium abscessus (


The Emergence Of Covid-19 Associated Mucormycosis: A Review Of Cases From 18 Countries, Martin Hoenigl, Danila Seidel, Agostinho Carvalho, Shivaprakash M. Rudramurthy, Amir Arastehfar, Jean-Pierre Gangneux, Nosheen Nasir, Alexandro Bonifaz, Javier Araiza, Nikolai Klimko Jan 2022

The Emergence Of Covid-19 Associated Mucormycosis: A Review Of Cases From 18 Countries, Martin Hoenigl, Danila Seidel, Agostinho Carvalho, Shivaprakash M. Rudramurthy, Amir Arastehfar, Jean-Pierre Gangneux, Nosheen Nasir, Alexandro Bonifaz, Javier Araiza, Nikolai Klimko

Department of Medicine

Reports of COVID-19-associated mucormycosis have been increasing in frequency since early 2021, particularly among patients with uncontrolled diabetes. Patients with diabetes and hyperglycaemia often have an inflammatory state that could be potentiated by the activation of antiviral immunity to SARS-CoV2, which might favour secondary infections. In this Review, we analysed 80 published and unpublished cases of COVID-19-associated mucormycosis. Uncontrolled diabetes, as well as systemic corticosteroid treatment, were present in most patients with COVID-19-associated mucormycosis, and rhino-orbital cerebral mucormycosis was the most frequent disease. Mortality was high at 49%, which was particularly due to patients with pulmonary or disseminated mucormycosis or …


Points Of Pride, Georgia Southern University Jan 2022

Points Of Pride, Georgia Southern University

Points of Pride (2013-2023)

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Efficacy And Safety Of Enasidenib And Azacitidine Combination In Patients With Idh2 Mutated Acute Myeloid Leukemia And Not Eligible For Intensive Chemotherapy, Sangeetha Venugopal, Koichi Takahashi, Naval Daver, Abhishek Maiti, Gautam Borthakur, Sanam Loghavi, Nicholas J Short, Maro Ohanian, Lucia Masarova, Ghayas Issa, Xuemei Wang, Bueso-Ramos Carlos, Musa Yilmaz, Tapan Kadia, Michael Andreeff, Farhad Ravandi, Marina Konopleva, Hagop M Kantarjian, Courtney D Dinardo Jan 2022

Efficacy And Safety Of Enasidenib And Azacitidine Combination In Patients With Idh2 Mutated Acute Myeloid Leukemia And Not Eligible For Intensive Chemotherapy, Sangeetha Venugopal, Koichi Takahashi, Naval Daver, Abhishek Maiti, Gautam Borthakur, Sanam Loghavi, Nicholas J Short, Maro Ohanian, Lucia Masarova, Ghayas Issa, Xuemei Wang, Bueso-Ramos Carlos, Musa Yilmaz, Tapan Kadia, Michael Andreeff, Farhad Ravandi, Marina Konopleva, Hagop M Kantarjian, Courtney D Dinardo

Faculty, Staff and Student Publications

Preclinically, enasidenib and azacitidine (ENA + AZA) synergistically enhance cell differentiation, and venetoclax (VEN), a small molecule Bcl2 inhibitor (i) is particularly effective in IDH2 mutated acute myeloid leukemia (IDH2mutAML). This open label phase II trial enrolled patients (pts) with documented IDH2mutAML. All patients received AZA 75 mg/m2/d x 7 d/cycle and ENA 100 mg QD continuously. Concomitant Bcl2i and FLT3i were allowed (NCT03683433).Twenty-six pts received ENA + AZA (median 68 years, range, 24-88); 7 newly diagnosed (ND) and 19 relapsed/refractory (R/R). In R/R AML patients, three had received prior ENA and none had received prior VEN. The …


Examining The Effect Of A Chitosan Biopolymer On Alkali-Activated Inorganic Material For Aqueous Pb(Ii) And Zn(Ii) Sorption, Sukanta K. Mondal, Chenglin Wu, Felix C. Nwadire, Ali A. Rownaghi, Aditya Kumar, Yusuf Adewuyi, Monday Uchenna Okoronkwo Jan 2022

Examining The Effect Of A Chitosan Biopolymer On Alkali-Activated Inorganic Material For Aqueous Pb(Ii) And Zn(Ii) Sorption, Sukanta K. Mondal, Chenglin Wu, Felix C. Nwadire, Ali A. Rownaghi, Aditya Kumar, Yusuf Adewuyi, Monday Uchenna Okoronkwo

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Biopolymers and alkali-activated materials have attracted a great deal of attention as adsorbents for the removal of heavy metal contaminants from aqueous solutions. Both materials are sustainable and feature unique properties, but biopolymers are relatively more expensive or difficult to prepare and exhibit low mechanical and surface properties, a narrow pH range, and thermal stability. In this study, hybrid adsorbents were prepared from both types of material, by alkali activation of low-cost fly ash precursors accompanied by incorporation of 0-2%mass chitosan biopolymer. Two types of alkaline activating solutions, NaOH and Na2SiO3, were employed to generate two sets of hybrid adsorbents …


Disruption Of Mecp2-Tcf20 Complex Underlies Distinct Neurodevelopmental Disorders, Jian Zhou, Hamdan Hamdan, Hari Krishna Yalamanchili, Kaifang Pang, Amy E Pohodich, Joanna Lopez, Yingyao Shao, Juan A Oses-Prieto, Lifang Li, Wonho Kim, Mark A Durham, Sameer S Bajikar, Donna J Palmer, Philip Ng, Michelle L Thompson, E Martina Bebin, Amelie J Müller, Alma Kuechler, Antje Kampmeier, Tobias B Haack, Alma L Burlingame, Zhandong Liu, Matthew N Rasband, Huda Y Zoghbi Jan 2022

Disruption Of Mecp2-Tcf20 Complex Underlies Distinct Neurodevelopmental Disorders, Jian Zhou, Hamdan Hamdan, Hari Krishna Yalamanchili, Kaifang Pang, Amy E Pohodich, Joanna Lopez, Yingyao Shao, Juan A Oses-Prieto, Lifang Li, Wonho Kim, Mark A Durham, Sameer S Bajikar, Donna J Palmer, Philip Ng, Michelle L Thompson, E Martina Bebin, Amelie J Müller, Alma Kuechler, Antje Kampmeier, Tobias B Haack, Alma L Burlingame, Zhandong Liu, Matthew N Rasband, Huda Y Zoghbi

Duncan NRI Faculty and Staff Publications

MeCP2 is associated with Rett syndrome (RTT), MECP2 duplication syndrome, and a number of conditions with isolated features of these diseases, including autism, intellectual disability, and motor dysfunction. MeCP2 is known to broadly bind methylated DNA, but the precise molecular mechanism driving disease pathogenesis remains to be determined. Using proximity-dependent biotinylation (BioID), we identified a transcription factor 20 (TCF20) complex that interacts with MeCP2 at the chromatin interface. Importantly, RTT-causing mutations in MECP2 disrupt this interaction. TCF20 and MeCP2 are highly coexpressed in neurons and coregulate the expression of key neuronal genes. Reducing Tcf20 partially rescued the behavioral deficits caused …


Characterization Of The Copd Alveolar Niche Using Single-Cell Rna Sequencing, Maor Sauler, John E Mcdonough, Taylor S Adams, Neeharika Kothapalli, Thomas Barnthaler, Rhiannon B Werder, Jonas C Schupp, Jessica Nouws, Matthew J Robertson, Cristian Coarfa, Tao Yang, Maurizio Chioccioli, Norihito Omote, Carlos Cosme, Sergio Poli, Ehab A Ayaub, Sarah G Chu, Klaus H Jensen, Jose L Gomez, Clemente J Britto, Micha Sam B Raredon, Laura E Niklason, Andrew A Wilson, Pascal N Timshel, Naftali Kaminski, Ivan O Rosas Jan 2022

Characterization Of The Copd Alveolar Niche Using Single-Cell Rna Sequencing, Maor Sauler, John E Mcdonough, Taylor S Adams, Neeharika Kothapalli, Thomas Barnthaler, Rhiannon B Werder, Jonas C Schupp, Jessica Nouws, Matthew J Robertson, Cristian Coarfa, Tao Yang, Maurizio Chioccioli, Norihito Omote, Carlos Cosme, Sergio Poli, Ehab A Ayaub, Sarah G Chu, Klaus H Jensen, Jose L Gomez, Clemente J Britto, Micha Sam B Raredon, Laura E Niklason, Andrew A Wilson, Pascal N Timshel, Naftali Kaminski, Ivan O Rosas

Faculty, Staff and Students Publications

Chronic obstructive pulmonary disease (COPD) is a leading cause of death worldwide, however our understanding of cell specific mechanisms underlying COPD pathobiology remains incomplete. Here, we analyze single-cell RNA sequencing profiles of explanted lung tissue from subjects with advanced COPD or control lungs, and we validate findings using single-cell RNA sequencing of lungs from mice exposed to 10 months of cigarette smoke, RNA sequencing of isolated human alveolar epithelial cells, functional in vitro models, and in situ hybridization and immunostaining of human lung tissue samples. We identify a subpopulation of alveolar epithelial type II cells with transcriptional evidence for aberrant …


Allogeneic Transplant And Car-T Therapy After Autologous Transplant Failure In Dlbcl: A Noncomparative Cohort Analysis, Mehdi Hamadani, Ajay K Gopal, Marcelo Pasquini, Soyoung Kim, Xianmiao Qiu, Sairah Ahmed, Aleksandr Lazaryan, Vijaya Raj Bhatt, Andrew Daly, Premal Lulla, Stefan Ciurea, Jordan Gauthier, Vaibhav Agrawal, Natalie S Grover, Lazaros Lekakis, Dipenkumar Modi, Parastoo B Dahi, Megan M Herr, P Connor Johnson, Hamza Hashmi, Peiman Hematti, Frederick L Locke Jan 2022

Allogeneic Transplant And Car-T Therapy After Autologous Transplant Failure In Dlbcl: A Noncomparative Cohort Analysis, Mehdi Hamadani, Ajay K Gopal, Marcelo Pasquini, Soyoung Kim, Xianmiao Qiu, Sairah Ahmed, Aleksandr Lazaryan, Vijaya Raj Bhatt, Andrew Daly, Premal Lulla, Stefan Ciurea, Jordan Gauthier, Vaibhav Agrawal, Natalie S Grover, Lazaros Lekakis, Dipenkumar Modi, Parastoo B Dahi, Megan M Herr, P Connor Johnson, Hamza Hashmi, Peiman Hematti, Frederick L Locke

Faculty, Staff and Students Publications

Allogeneic transplant (alloHCT) and chimeric antigen receptor modified (CAR)-T cell therapy are potentially cuarative options of diffuse large B-cell lymphoma (DLBCL) relapsing after an autologous (auto)HCT. Although the Center for International Blood and Marrow Transplant Research (CIBMTR) prognostic model can predict outcomes of alloHCT in DLBCL after autoHCT failure, corresponding models of CAR-T treatment in similar patient populations are not available. In this noncomparative registry analysis, we report outcomes of patients with DLBCL (≥18 years) undergoing a reduced intensity alloHCT or CAR-T therapy with axicabtagene ciloleucel during 2012 to 2019 after a prior auto-HCT failure and apply the CIBMTR prognostic …


Steroid-Sensitive Nephrotic Syndrome Candidate Gene Clvs1 Regulates Podocyte Oxidative Stress And Endocytosis, Brandon M. Lane, Andrew F. Malone, Et Al. Jan 2022

Steroid-Sensitive Nephrotic Syndrome Candidate Gene Clvs1 Regulates Podocyte Oxidative Stress And Endocytosis, Brandon M. Lane, Andrew F. Malone, Et Al.

2020-Current year OA Pubs

We performed next-generation sequencing in patients with familial steroid-sensitive nephrotic syndrome (SSNS) and identified a homozygous segregating variant (p.H310Y) in the gene encoding clavesin-1 (CLVS1) in a consanguineous family with 3 affected individuals. Knockdown of the clavesin gene in zebrafish (clvs2) produced edema phenotypes due to disruption of podocyte structure and loss of glomerular filtration barrier integrity that could be rescued by WT CLVS1 but not the p.H310Y variant. Analysis of cultured human podocytes with CRISPR/Cas9-mediated CLVS1 knockout or homozygous H310Y knockin revealed deficits in clathrin-mediated endocytosis and increased susceptibility to apoptosis that could be rescued with corticosteroid treatment, mimicking …


Forage Quality Of Cereal–Common Vetch At Different Age And Proportions, J. L. Zaragoza-Ramirez, P. A. Martinez-Hernandez, Ma. De L. Reyes-Medel Jan 2022

Forage Quality Of Cereal–Common Vetch At Different Age And Proportions, J. L. Zaragoza-Ramirez, P. A. Martinez-Hernandez, Ma. De L. Reyes-Medel

IGC Proceedings (1977-2023)

Cereal plant age at harvesting and the proportion of vetch in the harvested forage from cereal-common vetch mixtures might influence total forage quality. The objectives were to determine forage of a forage mixture cereal-vetch, from cereal harvested at two development stages and vetch at different proportion. Cereals were oats and triticale, cultivars: Chihuahua, Bicentenario and Siglo XXI, the last two were triticale; cereal development stages at harvest were: 50% flowering and hard grain. Vetch was harvested at two development stages: 100% flowering and pod formation, while vetch proportions in the forage mix were: 0, 0.25, 0.5, 0.75 and 1.0. Forage …


Forage Yield Of Oat On The Four Altitude Regions Of The Qinghai-Tibetan Plateau, H. B. Zhang, C. T. Liu, J. Q. Deng, X. L. Yang Jan 2022

Forage Yield Of Oat On The Four Altitude Regions Of The Qinghai-Tibetan Plateau, H. B. Zhang, C. T. Liu, J. Q. Deng, X. L. Yang

IGC Proceedings (1977-2023)

Forage oat (Avena sativa L.) for livestock in Qinghai-Tibet Plateau may be used biomass under widely different altitude conditions. To assess the effect of altitude on the biomass of oats, we conducted an experiment to explore the adaptability of forage oat Jiayan No. 2 in different altitudes for better agronomic traits and higher forage yield. Four regions included Hezheng county (2207 m a.s.l) and Tianzhu county (2407 m a.s.l), Gansu province, Haiyan county (3042 m a.s.l), Qinghai province and Hongyuan county (3460 m a.s.l), Sichuan province were selected, and agronomic data as well as dry matter yield were determined. …


Forage Production And Grazing Behaviour Of Beef Cattle In Agrosilvopastoral Systems In Brazil During The Dry Season, M. Pereira, P. Zawada, J. Werner, M. M. Macedo, R. G. Almeida, U. Dickhoefer Jan 2022

Forage Production And Grazing Behaviour Of Beef Cattle In Agrosilvopastoral Systems In Brazil During The Dry Season, M. Pereira, P. Zawada, J. Werner, M. M. Macedo, R. G. Almeida, U. Dickhoefer

IGC Proceedings (1977-2023)

We aimed at evaluating the microclimate, canopy structure, and grazing behaviour of cattle on Brachiaria sp. pastures within continuous pasture (CON), integrated crop-livestock (ICL), and crop-livestock-forestry (ICLF) systems in Brazilian dry season. Forage accumulation was monthly assessed in four paddocks per system from May to August 2019. To characterize the microclimate, air temperature and relative humidity were measured every hour and temperature-humidity index calculated. Grazing behaviour of 12 Nellore heifers per system (mean bodyweight 355 standard deviation±32.6 kg) was recorded by chewing sensors on nine continuous days (1 d adaptation + 8 d measurement) of three 15-d periods. Four animals …


Forage Accumulation And Quality Of Three Contrasting Ecotypes Of Tall Fescue (Schedonorus Arundinaceus) Managed Under Frequent Defoliation, J. Mattera, O. Scheneiter, I. Cattoni, E. Pacente, P. Barletta, O. Bertín Jan 2022

Forage Accumulation And Quality Of Three Contrasting Ecotypes Of Tall Fescue (Schedonorus Arundinaceus) Managed Under Frequent Defoliation, J. Mattera, O. Scheneiter, I. Cattoni, E. Pacente, P. Barletta, O. Bertín

IGC Proceedings (1977-2023)

In the past, tall fescue ecotypes were compared under intermediate management defoliation frequency, but now has station, Argentina (-33 ° 56 'S, -60 ° 33' W) in autumn 2016, on a typical Argiudoll soil. The treatments were cultivars representing three ecotypes of tall fescue: (i) - Mediterranean (cv. Flecha), (ii) - Intermediate (cv. Royal Q 100) and (iii) - Continental (cv. Lujan INTA). Each plot consisted of 7 rows 6.0 m long, 0.2 m apart (8.4m2 plot size) arranged in a randomized complete block design with 4 replicates. The first cut was done at the end of September when …


Benefits Of Cover Cropping Systems In Walnut Orchards As Sustainable Agricultural Practice, Ana Medic, Consuelo B. Baez Vega Jan 2022

Benefits Of Cover Cropping Systems In Walnut Orchards As Sustainable Agricultural Practice, Ana Medic, Consuelo B. Baez Vega

CSU Journal of Sustainability and Climate Change

In recent years walnut orchards implemented cover crops in between rows to improve soil’s quality, lessen soil’s erosion, increase organic matter, manage nutrient movement and availability, enhance water retention, and expand microbe, insect, and flora diversity. Commonly selected cover crops in California are from families Poaceae, Brassicaceae, and Fabaceae. Considerations should be made when choosing a particular cover crop mixture to enhance multiple benefits and improve sustainable practices in orchard settings. An experiment was conducted in a walnut orchard to compare functionality and benefits of three systems multi-crop, monocrop, and no vegetation cover crop system. The following components were evaluated: …