Digital Commons Network^™

Designing And Implementing A Novel Graduate Program To Develop Transdisciplinary Leaders In Urban Sustainability, Megan M. Wallen, Ingrid Guerra-Lopez, Louay Meroueh, Rayman Mohamed, Andrea Sankar, Pradeep Sopory, Ryan Watkins, Donna R. Kashian

Biological Sciences Faculty Research Publications

Urban settings, where >50% of the world's population resides, are increasingly faced with environmental challenges that threaten their sustainability. Aging infrastructure, water and air pollution, and increasing recognition of environmental injustices highlight the need for professionals to employ complex scientific reasoning across disciplines where they can effectively address the multifaceted issues of urban sustainability. Here we present an innovative model for preparing the next generation of public, private, and academic leaders to address complex problems in urban sustainability. Specifically, we outline the design and implementation of an integrated, adaptable graduate training program, with the goals of science leadership, curriculum relevancy, …

University Curriculum Committee Meeting Minutes - January 27, 2022, University Curriculum Committee

Faculty Senate Minutes

Minutes for the Governors State University Curriculum Committee Meeting held January 27, 2022.

A History Of Wright State University's Department Of Geological Science, Paul J. Wolfe

Earth and Environmental Sciences Faculty Publications

A history of Wright State University's disbanded and reintegrated Department of Geological Sciences written by department faculty member Paul J. Wolfe. Wolfe describes the development of the program, the faculty throughout the years, and the programs offered through the department.

Computational Genomics In The Era Of Precision Medicine: Applications To Variant Analysis And Gene Therapy, Yung-Chun Wang, Julie Choi, Shujuan Zhao, Mariam Khanfar, Kuangying Yang, Po-Ying Fu, Max Wrubel, Xiaobing Yu, Sheng Chih Jin, Et Al

Open Access Publications

Rapid methodological advances in statistical and computational genomics have enabled researchers to better identify and interpret both rare and common variants responsible for complex human diseases. As we continue to see an expansion of these advances in the field, it is now imperative for researchers to understand the resources and methodologies available for various data types and study designs. In this review, we provide an overview of recent methods for identifying rare and common variants and understanding their roles in disease etiology. Additionally, we discuss the strategy, challenge, and promise of gene therapy. As computational and statistical approaches continue to …

A Comprehensive Evaluation Of Wastewater Grit Characteristics And Its Application In The Design Of A Facility-Specific Grit Removal Solution, Tetiana Kolsovska

Theses and Dissertations

Tailored solutions for wastewater treatment plants (WWTPs) are becoming more prevalent for optimization of treatment process, based on the unique quality of the influent received by each facility. In-depth evaluation of the influent properties is necessary for design of the most appropriate treatment system. In this study, wastewater grit was evaluated in terms of its quantity, particle size distribution, total organic carbon (TOC) content, including fats, oils, and grease (FOG), and settling velocity of the particles, over the course of one year at the headworks, collection network, and through the treatment stages of the Atlantic County Utilities Authority (ACUA) WWTP, …

Kinetic Study Of The Anaerobic Digestion Of Recycled Paper Mill Effluent (Rpme) By Using A Novel Modified Anaerobic Hybrid Baffled (Mahb) Reactor, Siti Roshayu Hassan, Yung-Tse Hung, Irvan Dahlan, Hamidi Abdul Aziz

Civil and Environmental Engineering Faculty Publications

The process kinetics of an anaerobic digestion process for treating recycled paper mill effluent (RPME) was investigated. A laboratory-scale modified anaerobic hybrid baffled reactor (MAHB) was operated at hydraulic retention times of 1, 3, 5, and 7 days, and the results were analyzed for the kinetic models. A kinetic study was conducted by examining the phase kinetics of the anaerobic digestion process, which were divided into three main stages: hydrolysis kinetics, acetogenesis kinetics, and methane production kinetics. The study demonstrated that hydrolysis was the rate-limiting step. The applied Monod and Contois kinetic models showed satisfactory prediction with µmax values of …

Understanding Quality Of Life In Adolescents Living With Advanced Cancer, Cynthia J. Bell

Dissertations

The purpose of this study is to advance theoretical understanding of how an adolescent with incurable cancer prepares for end-of-life (EOL). A theoretical model was developed to link awareness, acceptance, and willingness to take action to EOL preparedness (knowledge about EOL, acknowledgement of grief and emotion, identification of the meaning of death and spirituality, and conceptualization of personal plan) and communication about EOL preferences and priorities; and to determine impact on quality of life (QOL).

Understanding The Relationship Between Genetic Markers And Skeletal Remains: Implications For Forensic Anthropology And Phenotype-Genotype Studies, Kamar Afra, Bridget F.B. Algee-Hewitt, Michelle D. Hamilton

Human Biology Open Access Pre-Prints

Human identification techniques have been a leading tool to hold perpetrators accountable, give families closure, and reconstruct faces on skulls. This project is a pilot study to critically examine three disciplines that fall under the human identification umbrella: forensic anthropology, forensic genetics, and forensic art. Current facial research in genetics focuses on data from living individuals where specific single-nucleotide polymorphisms (SNPs) that influence specific regions of the face have been found. This study assesses the translation of these same regions to craniometric dimensions (inter-landmark distances) of the underlying skull itself. The goal of this project is to provide information regarding …

Form Of Supplemental Selenium Affects The Expression Of Mrna Transcripts Encoding Selenoproteins, And Proteins Regulating Cholesterol Uptake, In The Corpus Luteum Of Grazing Beef Cows, Sarah N. Carr, Benjamin R. Crites, Joy L. Pate, Camilla H. K. Hughes, James C. Matthews, Phillip J. Bridges

Animal and Food Sciences Faculty Publications

Selenium (Se)-deficient soils necessitate supplementation of this mineral to the diet of forage-grazing cattle. Functionally, Se is incorporated into selenoproteins, some of which function as important antioxidants. We have previously shown that the source of supplemental Se; inorganic (sodium selenite or sodium selenate; ISe), organic (selenomethionine or selenocysteine; OSe) or 1:1 mix of ISe and OSe (MIX), provided to Angus-cross cows affects concentrations of progesterone (P4) during the early luteal phase of the estrous cycle. In this study, we sought to investigate (1) the effect of form of Se on the expression of mRNA encoding selenoproteins in the corpus luteum …

The Coxsackievirus And Adenovirus Receptor Has A Short Half-Life In Epithelial Cells, Poornima Kotha Lakshmi Narayan, James M. Readler, Mahmoud S. Alghamri, Trisha L. Brockman, Ray Yan, Priyanka Sharma, Vladislav Snitsarev, Katherine J.D.A Excoffon, Abimbola O. Kolawole

Department of Biology Faculty Scholarship and Creative Works

The coxsackievirus and adenovirus receptor (CAR) is an essential cellular protein that is involved in cell adhesion, cell signaling, and viral infection. The 8-exon encoded isoform (CAREx8) resides at the apical surface of polarized epithelia, where it is accessible as a receptor for adenovirus entering the airway lumen. Given its pivotal role in viral infection, it is a target for antiviral strategies. To understand the regulation of CAREx8 and determine the feasibility of receptor down regulation, the half-life of total and apical localized CAREx8 was determined and correlated with adenovirus transduction. Total and apical CAREx8 has a relatively short half-life …

Reply To Comment: Controls On Turnover Of Marine Dissolved Organic Matter-Testing The Null Hypothesis Of Purely Concentration-Driven Uptake, Yuan Shen, Ronald Benner

Faculty Publications

Our recent bioassay experiments indicate that molecular properties are a primary control on the microbial utilization of dissolved organic matter in the ocean. This finding is questioned by Lennartz and Dittmar who modeled our experiments and concluded that our observations could be largely explained by concentration-driven uptake independent from molecular properties. We suggest the authors' models are deficient for establishing the relative roles of molecular properties and concentration-driven uptake. Our conclusion is consistent with earlier and recent experimental results and biogeochemical observations, supporting a unified theory with molecular properties as a more prominent control than concentration-driven uptake on marine organic …

Form Of Supplemental Selenium Affects The Expression Of Mrna Transcripts Encoding Selenoproteins, And Proteins Regulating Cholesterol Uptake, In The Corpus Luteum Of Grazing Beef Cows, Sarah Carr, Benjamin R. Crites, Joy Pate, Camilla Hughes, James C. Matthews, Phillip J. Bridges

Animal and Food Sciences Graduate Research

Selenium (Se)-deficient soils necessitate supplementation of this mineral to the diet of forage-grazing cattle. Functionally, Se is incorporated into selenoproteins, some of which function as important antioxidants. We have previously shown that the source of supplemental Se; inorganic (sodium selenite or sodium selenate; ISe), organic (selenomethionine or selenocysteine; OSe) or 1:1 mix of ISe and OSe (MIX), provided to Angus-cross cows affects concentrations of progesterone (P4) during the early luteal phase of the estrous cycle. In this study, we sought to investigate (1) the effect of form of Se on the expression of mRNA encoding selenoproteins in the corpus luteum …

The Impact Of Led Lighting Spectra In A Plant Factory On The Growth, Physiological Traits And Essential Oil Content Of Lemon Balm (Melissa Officinalis), Hail Z. Rihan, Naofel Aljafer, Marwa Jbara, Lynn Mccallum, Sabine Lengger, Michael P. Fuller

School of Biological and Marine Sciences

With the recent development of LED lighting systems for plant cultivation, the use of vertical farming under controlled conditions is attracting increased attention. This study investigated the impact of a number of LED light spectra (red, blue, green and white) on the growth, development and essential oil content of lemon balm (Melissa officinalis), a herb and pharmaceutical plant species used across the world. White light and red-rich light spectra gave the best outputs in terms of impact on the growth and yield. For blue-rich spectra, the development and yield was lower despite having a significant impact on the photosynthesis activity, …

Gut Microbial Trimethylamine Is Elevated In Alcohol-Associated Hepatitis And Contributes To Ethanol-Induced Liver Injury In Mice, Robert N. Helsley, Tatsunori Miyata, Anagha Kadam, Venkateshwari Varadharajan, Naseer Sangwan, Emily C. Huang, Rakhee Banerjee, Amanda L. Brown, Kevin K. Fung, William J. Massey, Chase Neumann, Danny Orabi, Lucas J. Osborn, Rebecca C. Schugar, Megan R. Mcmullen, Annette Bellar, Kyle L. Poulsen, Adam Kim, Vai Pathak, Marko Mrdjen

Pediatrics Faculty Publications

There is mounting evidence that microbes residing in the human intestine contribute to diverse alcohol-associated liver diseases (ALD) including the most deadly form known as alcohol-associated hepatitis (AH). However, mechanisms by which gut microbes synergize with excessive alcohol intake to promote liver injury are poorly understood. Furthermore, whether drugs that selectively target gut microbial metabolism can improve ALD has never been tested. We used liquid chromatography tandem mass spectrometry to quantify the levels of microbe and host choline co-metabolites in healthy controls and AH patients, finding elevated levels of the microbial metabolite trimethylamine (TMA) in AH. In subsequent studies, we …

Human Microrna (Mir-20b-5p) Modulates Alzheimer’S Disease Pathways And Neuronal Function, And A Specific Polymorphism Close To The Mir20b Gene Influences Alzheimer’S Biomarkers, Ruizhi Wang, Nipun Chopra, Kwangsik Nho, Bryan Maloney, Alexander G. Obukhov, Peter T. Nelson, Scott E. Counts, Debomoy K. Lahiri

Sanders-Brown Center on Aging Faculty Publications

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with loss of cognitive, executive, and other mental functions, and is the most common form of age-related dementia. Amyloid-β peptide (Aβ) contributes to the etiology and progression of the disease. Aβ is derived from the amyloid-β precursor protein (APP). Multiple microRNA (miRNA) species are also implicated in AD. We report that human hsa-miR20b-5p (miR-20b), produced from the MIR20B gene on Chromosome X, may play complex roles in AD pathogenesis, including Aβ regulation. Specifically, miR-20b-5p miRNA levels were altered in association with disease progression in three regions of the human brain: temporal neocortex, …

Toward Understanding Bacterial Ice Nucleation, Max Lukas, Ralph Schwidetzky, Rosemary J. Eufemio, Mischa Bonn, Konrad Meister

Faculty, Staff, and Students

Bacterial ice nucleators (INs) are among the most effective ice nucleators known and are relevant for freezing processes in agriculture, the atmosphere, and the biosphere. Their ability to facilitate ice formation is due to specialized ice-nucleating proteins (INPs) anchored to the outer bacterial cell membrane, enabling the crystallization of water at temperatures up to −2 °C. In this Perspective, we highlight the importance of functional aggregation of INPs for the exceptionally high ice nucleation activity of bacterial ice nucleators. We emphasize that the bacterial cell membrane, as well as environmental conditions, is crucial for a precise functional INP aggregation. Interdisciplinary …

Variation In Trophic Niches Of Fish In Lake Powell Prior To Full Colonization By Quagga Mussel (Dreissena Rostriformis Bugensis), Joshua A. Verde, Nathan R. St. Andre, Mark C. Belk

Western North American Naturalist

Invasive species can cause disruption of the trophic niche of resident species such that patterns of energy flow through ecological systems are altered. Quagga mussel Dreissena rostriformis bugensis Andrusov, 1897, a notorious invasive species, was introduced to Lake Powell (Utah and Arizona, USA) in 2012 and colonized the entire reservoir by 2017. To help understand potential effects of this invasive species on the trophic niche of fishes in Lake Powell, we characterized trophic niche of fishes and other organisms, as well as sources of variation in trophic niche, using stable isotope analysis of carbon and nitrogen prior to full colonization …

Genotype Frequency Of The Common Tlr4 Snps In A Kurdish Population: Global Reviews And Out-Of-African Migrations, Sherko Subhan Niranji, Sirwan M. Amin Al Jaf

Human Biology Open Access Pre-Prints

Toll-like receptors (TLRs) are cellular innate immune receptors that explore microbial molecules. For instance, TLR4 can sense bacterial lipopolysaccharides (LPS) inducing cytokines and anti-microbial peptides against the bacteria. It has been shown that single nucleotide polymorphisms (SNPs) in TLR4 are associated with diseases such as septic shock. Therefore, investigations of common SNPs may help to explain the pathogenesis of diseases and various innate immune responses against infections. This study investigates genotypic frequencies of two common TLR4 SNPs (Asp299Gly and Thr399Ile) in a Kurdish population using Restriction Length Fragment Polymorphisms (RFLP). Furthermore, the global frequencies of both TLR4 SNPs are reviewed …

Variation In American Bison (Bison Bison) Ecological Functionality Across Management Sectors: Implications For Conducting Conservation Assessments, Luke Rollie Rogers

Biology Theses, Dissertations, and Student Creative Activity

The American bison (Bison bison subspp.), once on the verge of extinction, now number in the hundreds of thousands. Despite an understanding of their numerical recovery, a comprehensive survey of bison herds and how their management varies between sectors has never been completed, and neither has a critical evaluation of the level of ecological recovery of the species. I surveyed bison managers from all major sectors, collecting extensive information about each bison situation. I identified significant proportional differences between management sectors among my survey questions using Freeman-Halton-Fisher exact tests of independence. I visualized and grouped individual herds based on their …

Measurement Of Binding Affinity And Receptor Activation Of A Novel Curcumin Analog, Cis-Trans Curcumin, At Adenosine Receptors A1, A2a, A2b, And A3, Luke James Hamilton

Biology Theses, Dissertations, and Student Creative Activity

Vanilloid phytochemicals (VPs), which contain the 4-hydroxy-3-methoxybenzyl group, have consistently been shown to alleviate pain. All four of the adenosine receptor (AR) subtypes mediate pain and have been targeted by pharmacologists to generate new therapeutics for pain. However, despite the fact that both VPs and ARs are connected to pain relief, only a few studies have described the interaction between VPs and ARs. Furthermore, the few studies that have been done have all been performed in vivo, with no assessment of binding affinity and receptor activation in vitro. In this study, photochemical methods were used to generate a novel isomer …

Crossroads Of Drug Abuse And Hiv Infection: Neurotoxicity And Cns Reservoir, Shilpa Sonti, Kratika Tyagi, Amit Pande, René Daniel, Adhikarimayum Lakhikumar Sharma, Mudit Tyagi

Center for Translational Medicine Faculty Papers

Drug abuse is a common comorbidity in people infected with HIV. HIV-infected individuals who abuse drugs are a key population who frequently experience suboptimal outcomes along the HIV continuum of care. A modest proportion of HIV-infected individuals develop HIV-associated neurocognitive issues, the severity of which further increases with drug abuse. Moreover, the tendency of the virus to go into latency in certain cellular reservoirs again complicates the elimination of HIV and HIV-associated illnesses. Antiretroviral therapy (ART) successfully decreased the overall viral load in infected people, yet it does not effectively eliminate the virus from all latent reservoirs. Although ART increased …

The Montclarion, January 27, 2022, The Montclarion

The Montclarion

Student Newspaper of Montclair State University

Implementation Of Epigenetic Variation In Sorghum Selection And Implications For Crop Resilience Breeding, Dikungwa Ketumile, Xiaodong Yang, Robersy Sanchez, Hardik Kundariya, John F. Rajewski, Ismail M. Dweikat, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Crop resilience and yield stability are complex traits essential for food security. Sorghum bicolor is an important grain crop that shows promise for its natural resilience to drought and potential for marginal land production. We have developed sorghum lines in the Tx430 genetic background suppressed for MSH1 expression as a means of inducing de novo epigenetic variation, and have used these materials to evaluate changes in plant growth vigor. Plant crossing and selection in two distinct environments revealed features of phenotypic plasticity derived from MSH1 manipulation. Introduction of an epigenetic variation to an isogenic sorghum population, in the absence of …

Effects Of Long-Term Fluoride Exposure Are Associated With Oxidative Biochemistry Impairment And Global Proteomic Modulation, But Not Genotoxicity, In Parotid Glands Of Mice, Giza Hellen Nonato Mirandai, Leidiane Alencar De Oliveira Lima, Leonardo Oliveira Bittencourt, Sa´Vio Monteiro Dos Santos, Michel Platini Caldas De Souza, Lygia Sega Nogueira, Edivaldo Herculano Corrêa De Oliveira, Marta Chagas Monteir, Aline Dionizio, Aline Lima Leite, Juliano Pelim Pessan, Juliano Pelim Pessan, Marı´Lia Afonso Rabelo Buzalaf, Rafael Rodrigues Limai

Department of Chemistry: Faculty Publications

Fluoride has become widely used in dentistry because of its effectiveness in caries control. However, evidence indicates that excessive intake interferes with the metabolic processes of different tissues. Thus, this study aimed to investigate the effects of long-term exposure to F on the parotid salivary gland of mice, from the analysis of oxidative, proteomic and genotoxic parameters.

The animals received deionized water containing 0, 10 or 50 mg/L of F, as sodium fluoride, for 60 days. After, parotid glands were collected for analysis of oxidative biochemistry, global proteomic profile, genotoxicity assessment and histopathological analyses.

The results revealed that exposure to …

Transcriptional Coactivators: Driving Force Of Plant Immunity, Muhammad Saad Shoaib Khan, Faisal Islam, Huan Chen, Ming Chang, Daowen Wang, Fengquan Liu, Zhengqing Fu, Jian Chen

Faculty Publications

Salicylic acid (SA) is a plant defense signal that mediates local and systemic immune responses against pathogen invasion. However, the underlying mechanism of SA-mediated defense is very complex due to the involvement of various positive and negative regulators to fine-tune its signaling in diverse pathosystems. Upon pathogen infections, elevated level of SA promotes massive transcriptional reprogramming in which Non-expresser of PR genes 1 (NPR1) acts as a central hub and transcriptional coactivator in defense responses. Recent findings show that Enhanced Disease Susceptibility 1 (EDS1) also functions as a transcriptional coactivator and stimulates the expression of PR1 in the presence of …

Effects Of Exercise On Physiologic And Psychologic Outcomes In Patients With End Stage Renal Disease On Hemodialysis: A Quasi-Experimental Study In Lebanon, Ghada Ballout Dunbar

Dissertations

End stage renal disease (ESRD) is a public health problem that is expected to continue to rise and has overwhelming effects on individuals' psychological, physical, and emotional wellbeing. Treatment options include hemodialysis (HD), which is a lifesaving procedure for patients suffering from ESRD where a dialysis machine and a dialyzer are used to clean and purify the blood as their kidneys are not functioning normally. This patient population will suffer from muscle atrophy and weakness due to inactive lifestyle and lack of physical activity. They also question their existence, are extremely stressed, and are afraid of death.

Threats To Internal Validity In Multiple-Baseline Design Variations, Timothy A. Slocum, Sarah E. Pinkelman, P. Raymond Joslyn, Beverly Nichols

Special Education and Rehabilitation Counseling Faculty Publications

Multiple baseline designs—both concurrent and nonconcurrent—are the predominant experimental design in modern applied behavior analytic research and are increasingly employed in other disciplines. In the past, there was significant controversy regarding the relative vigor of concurrent and nonconcurrent multiple baseline designs. The consensus in recent textbooks and methodological papers is that nonconcurrent designs are less rigorous than concurrent designs because of their presumed limited ability to address the threat of coincidental events (i.e., history). This skepticism of nonconcurrent designs stems from an emphasis on the importance of across-tier comparisons and relatively low importance placed on replicated within-tier comparisons for addressing …

Identification Of A Novel Glucuronyltransferase From Streptomyces Chromofuscus Atcc 49982 For Patural Product Glucuronidation, Jie Ren, Caleb Don Barton, Kathryn Eternity Sorenson, Jixun Zhan

Biological Engineering Faculty Publications

Glycosylation is an effective way to increase the polarity of natural products. UDP-glucuronyltransferases (UGTs) are commonly observed and extensively studied in phase II drug metabolism. However, UGTs in microorganisms are not well studied, which hampered the utilization of this type of enzyme in microbial glucuronidation of natural products. Screening of five actinomycete strains showed that Streptomyces chromofuscus ATCC 49982 can convert diverse plant polyphenols into more polar products, which were characterized as various glucuronides based on their spectral data. Analysis of the genome of this strain revealed a putative glucuronidation gene cluster that contains a UGT gene (gcaC) …

Importance Of Csf-Based Aβ Clearance With Age In Humans Increases With Declining Efficacy Of Blood-Brain Barrier/Proteolytic Pathways, Donald L Elbert, Bruce W Patterson, Brendan P Lucey, Tammie L S Benzinger, Randall J Bateman

Open Access Publications

The kinetics of amyloid beta turnover within human brain is still poorly understood. We previously found a dramatic decline in the turnover of Aβ peptides in normal aging. It was not known if brain interstitial fluid/cerebrospinal fluid (ISF/CSF) fluid exchange, CSF turnover, blood-brain barrier function or proteolysis were affected by aging or the presence of β amyloid plaques. Here, we describe a non-steady state physiological model developed to decouple CSF fluid transport from other processes. Kinetic parameters were estimated using: (1) MRI-derived brain volumes, (2) stable isotope labeling kinetics (SILK) of amyloid-β peptide (Aβ), and (3) lumbar CSF Aβ concentration …

Cytogenetics Of Black Fly Larvae (Diptera: Simuliidae) From The Logan River, Utah, The Type Locality Of Simulium Brevicercum, Gerald F. Shields

Western North American Naturalist

I analyzed 221 black fly larvae of the Simulium arcticum complex from the Logan River, Logan Canyon, Utah, for larval sexual dimorphism, chromosomal inversions, morphology of the centromere band of chromosome IIL, presence of B chromosomes, and frequency of the IS-1 autosomal polymorphism. All 2275 larvae collected at the Logan River were of the S. arcticum complex with one exception, which was S. canadense. I found a significant association between larval color and sex, but I did not find such an association between larval head patterns and sex. Nearly all chromosomally analyzed larvae had standard, noninverted IIL chromosomes in …