Digital Commons Network^™

Clinical Outcomes And Bacterial Characteristics Of Carbapenem-Resistant Klebsiella Pneumoniae Complex Among Patients From Different Global Regions (Crackle-2): A Prospective, Multicentre, Cohort Study, Minggui Wang, Michelle Earley, Liang Chen, Blake M Hanson, Yunsong Yu, Zhengyin Liu, Soraya Salcedo, Eric Cober, Lanjuan Li, Souha S Kanj, Hainv Gao, Jose M Munita, Karen Ordoñez, Greg Weston, Michael J Satlin, Sandra L Valderrama-Beltrán, Kalisvar Marimuthu, Martin E Stryjewski, Lauren Komarow, Courtney Luterbach, Steve H Marshall, Susan D Rudin, Claudia Manca, David L Paterson, Jinnethe Reyes, Maria V Villegas, Scott Evans, Carol Hill, Rebekka Arias, Keri Baum, Bettina C Fries, Yohei Doi, Robin Patel, Barry N Kreiswirth, Robert A Bonomo, Henry F Chambers, Vance G Fowler, Cesar A Arias, David Van Duin, Multi-Drug Resistant Organism Network Investigators

Faculty, Staff and Student Publications

BACKGROUND: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a global threat. We therefore analysed the bacterial characteristics of CRKP infections and the clinical outcomes of patients with CRKP infections across different countries.

METHODS: In this prospective, multicentre, cohort study (CRACKLE-2), hospitalised patients with cultures positive for CRKP were recruited from 71 hospitals in Argentina, Australia, Chile, China, Colombia, Lebanon, Singapore, and the USA. The first culture positive for CRKP was included for each unique patient. Clinical data on post-hospitalisation death and readmission were collected from health records, and whole genome sequencing was done on all isolates. The primary outcome was a desirability …

Type 2 Diabetes Partitioned Polygenic Scores Associate With Disease Outcomes In 454,193 Individuals Across 13 Cohorts, Daniel Dicorpo, Jessica Leclair, Joanne B Cole, Chloé Sarnowski, Fariba Ahmadizar, Lawrence F Bielak, Anneke Blokstra, Erwin P Bottinger, Layal Chaker, Yii-Der I Chen, Ye Chen, Paul S De Vries, Tariq Faquih, Mohsen Ghanbari, Valborg Gudmundsdottir, Xiuqing Guo, Natalie R Hasbani, Dorina Ibi, M Arfan Ikram, Maryam Kavousi, Hampton L Leonard, Aaron Leong, Josep M Mercader, Alanna C Morrison, Girish N Nadkarni, Mike A Nalls, Raymond Noordam, Michael Preuss, Jennifer A Smith, Stella Trompet, Petra Vissink, Jie Yao, Wei Zhao, Eric Boerwinkle, Mark O Goodarzi, Vilmundur Gudnason, J Wouter Jukema, Sharon L R Kardia, Ruth J F Loos, Ching-Ti Liu, Alisa K Manning, Dennis Mook-Kanamori, James S Pankow, H Susan J Picavet, Naveed Sattar, Eleanor M Simonsick, W M Monique Verschuren, Ko Willems Van Dijk, Jose C Florez, Jerome I Rotter, James B Meigs, Josée Dupuis, Miriam S Udler

Faculty, Staff and Student Publications

OBJECTIVE: Type 2 diabetes (T2D) has heterogeneous patient clinical characteristics and outcomes. In previous work, we investigated the genetic basis of this heterogeneity by clustering 94 T2D genetic loci using their associations with 47 diabetes-related traits and identified five clusters, termed β-cell, proinsulin, obesity, lipodystrophy, and liver/lipid. The relationship between these clusters and individual-level metabolic disease outcomes has not been assessed.

RESEARCH DESIGN AND METHODS: Here we constructed individual-level partitioned polygenic scores (pPS) for these five clusters in 12 studies from the Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) consortium and the UK Biobank (n = 454,193) …

Sociotechnical Safeguards For Genomic Data Privacy, Ellen W. Clayton, Zhiyu Wan, Et Al.

Vanderbilt Law School Faculty Publications

Recent developments in a variety of sectors, including health care, research and the direct-to-consumer industry, have led to a dramatic increase in the amount of genomic data that are collected, used and shared. This state of affairs raises new and challenging concerns for personal privacy, both legally and technically. This Review appraises existing and emerging threats to genomic data privacy and discusses how well current legal frameworks and technical safeguards mitigate these concerns. It concludes with a discussion of remaining and emerging challenges and illustrates possible solutions that can balance protecting privacy and realizing the benefits that result from the …

The Future Of Law And Neuroscience, Owen D. Jones

Vanderbilt Law School Faculty Publications

I was asked to speculate about where the field of Law and Neuroscience may be ten years from now. In that spirit (and while recognizing that the future rarely complies with our predictions) I attempt here some extrapolations. I first consider potential advances in the technologies for monitoring and manipulating brain states, the techniques for analyzing brain data, and the efforts to further integrate relevant fields. I then consider potential neurolaw developments relevant to: (1) detecting things law cares about; (2) individualizing developmental states and brain states; (3) evidence-based legal reforms; (4) legal decision-making; and (5) brain-brain interfaces.

Lytic Bacteriophages And Lactic Acid As Processing Aids Against Salmonella Spp. And Escherichia Coli O157:H7 On Marinated And Tenderized Pork Loins, Sherita Li

Master's Theses

Within the last decade, pork consumption has steadily increased and continues to be the most consumed meat globally. However, pathogenic bacterial strains resistant to antibiotics have also been increasingly found in pig farms, animals, and the environment. Bacterial food poisoning cases due to Salmonella spp. and Escherichia coli (E. coli) O157:H7 appear to be linked with a variety of pork products. The meat industry has recognized that research is needed to combat the multi-drug resistance in foodborne pathogens with alternative methods of control. This study evaluated the effects of both E. coli- and Salmonella-specific lytic bacteriophages and …

Nohwere, Peter A. Alces, Robert M. Sapolsky

William & Mary Law Review

Imagine the frustration of Samuel Butler’s protagonist, Higgs, with the strange society he encounters in Erewhon:

"Was there nothing which I could say to make them feel that the constitution of a person’s body was a thing over which he or she had had at any rate no initial control whatever, while the mind was a perfectly different thing, and capable of being created anew and directed according to the pleasure of its possessor? Could I never bring them to see that while habits of mind and character were entirely independent of initial mental force and early education, the body …

The Future Of Law And Neuroscience, Owen D. Jones

William & Mary Law Review

I was asked to speculate about where the field of Law and Neuroscience may be ten years from now. In that spirit (and while recognizing that the future rarely complies with our predictions) I attempt here some extrapolations. I first consider potential advances in the technologies for monitoring and manipulating brain states, the techniques for analyzing brain data, and the efforts to further integrate relevant fields. I then consider potential neurolaw developments relevant to: (1) detecting things law cares about; (2) individualizing developmental states and brain states; (3) evidence-based legal reforms; (4) legal decision-making; and (5) brain-brain interfaces.

A Safe Culture For Neuroscience, Bruce Waller

William & Mary Law Review

When examining the future impact of neuroscience on the law, the first step requires narrowing the scope of the inquiry: advances in neuroscience are exciting, but the beneficial or harmful effects of those advances will depend on the specific culture in which they occur. In some cultures—such as in Norway or Sweden—integrating advances in neuroscience into the criminal justice system is likely to enhance understanding and improve the treatment of offenders and potential offenders. In the neoliberal culture of the United States, advances are more likely to exacerbate the profound wrongs of the criminal justice system rather than ameliorate them. …

Elucidating The Clinical Spectrum And Molecular Basis Of Hyal2 Deficiency, James Fasham, Siying Lin, Promita Ghosh, Francesca Clementina Radio, Emily G Farrow, Isabelle Thiffault, Jennifer Kussman, Dihong Zhou, Rick Hemming, Kenneth Zahka, Barry A Chioza, Lettie E Rawlins, Olivia K Wenger, Adam C Gunning, Simone Pizzi, Roberta Onesimo, Giuseppe Zampino, Emily Barker, Natasha Osawa, Megan Christine Rodriguez, Teresa M Neuhann, Elaine H Zackai, Beth Keena, Jenina Capasso, Alex V Levin, Elizabeth Bhoj, Dong Li, Hakon Hakonarson, Ingrid M Wentzensen, Adam Jackson, Kate E Chandler, Zeynep H Coban-Akdemir, Jennifer E Posey, Siddharth Banka, James R Lupski, Sarah E Sheppard, Marco Tartaglia, Barbara Triggs-Raine, Andrew H Crosby, Emma L Baple

Faculty, Staff and Student Publications

PURPOSE: We previously defined biallelic HYAL2 variants causing a novel disorder in 2 families, involving orofacial clefting, facial dysmorphism, congenital heart disease, and ocular abnormalities, with Hyal2 knockout mice displaying similar phenotypes. In this study, we better define the phenotype and pathologic disease mechanism.

METHODS: Clinical and genomic investigations were undertaken alongside molecular studies, including immunoblotting and immunofluorescence analyses of variant/wild-type human HYAL2 expressed in mouse fibroblasts, and in silico modeling of putative pathogenic variants.

RESULTS: Ten newly identified individuals with this condition were investigated, and they were associated with 9 novel pathogenic variants. Clinical studies defined genotype-phenotype correlations and …

Post-Acquisition Hyperpolarized 29silicon Magnetic Resonance Image Processing For Visualization Of Colorectal Lesions Using A User-Friendly Graphical Interface, Caitlin V Mccowan, Duncan Salmon, Jingzhe Hu, Shivanand Pudakalakatti, Nicholas Whiting, Jennifer S Davis, Daniel D Carson, Niki M Zacharias, Pratip K Bhattacharya, Mary C Farach-Carson

Faculty, Staff and Student Publications

Medical imaging devices often use automated processing that creates and displays a self-normalized image. When improperly executed, normalization can misrepresent information or result in an inaccurate analysis. In the case of diagnostic imaging, a false positive in the absence of disease, or a negative finding when disease is present, can produce a detrimental experience for the patient and diminish their health prospects and prognosis. In many clinical settings, a medical technical specialist is trained to operate an imaging device without sufficient background information or understanding of the fundamental theory and processes involved in image creation and signal processing. Here, we …

A Privacy-Preserving National Clinical Data Warehouse: Architecture And Analysis, Md Raihan Mia, Abu Sayed Md Latiful Hoque, Shahidul Islam Khan, Sheikh Iqbal Ahamed

Computer Science Faculty Research and Publications

A centralized clinical data repository is essential for inspecting patients’ medical history, disease analysis, population-wide disease research, treatment decision support, and improving existing healthcare policies and services. Bangladesh, a rapidly developing country, poses several unusual challenges for developing such a centralized clinical data repository as the existing Electronic Health Records (EHR) are stored in unconnected, heterogeneous sources with no unique patient identifier and consistency. Data integration with secure record linkage, privacy preservation, quality control, and data standardization are the main challenges for developing a consistent and interoperable centralized clinical data repository. Based on the findings from our previous researches, we …

Comparison Of The Gad-7 And Impact Symptom Cluster Scores In Measuring Anxiety Among College Athletes, Robert Steven Cruz

Theses and Dissertations

Assessing the needs of mental health among college athletes is important given the prevalence rates, stigma, and complications that may arise after experiencing a concussion. Utilizing the General Anxiety Disorder- 7 (GAD-7) as an independent measure of anxiety at concussion baseline evaluation was explored among Division II collegiate athletes (n=568), which also included the Immediate Post-Concussion Assessment and Cognitive Testing (ImPACT) symptom clusters. ImPACT symptom clusters can be divided into four cluster (affective, cognitive, physical/somatic, and sleep) encompassing a 22-item self-report questionnaire. Simple linear regressions revealed that GAD-7 total score was significantly predicted by all four ImPACT symptom clusters. The …

Differences In List Learning Performance On The Moca Wordlist And Shepherd Verbal Learning Test In Cognitively Normal, Mci, And Ad Individuals, Gabrielle Montgomery Gavitt

Theses and Dissertations

Title: Differences in List Learning Performance on the MoCA wordlist and Shepherd Verbal Learning Test in Cognitively Normal, MCI, and AD Individuals Author: Gabrielle Montgomery Gavitt, M.S. Major Advisor: Anthony LoGalbo, Ph.D. Objective: The present study examines performance on the MoCA wordlist and SVLT wordlist and their association with the outcomes of healthy, MCI, and AD in a memory disorder clinic sample and community sample. Method: Archival data form 125 Health First Memory Disorder Clinic patients was utilized. Data from a community sample of geriatric individuals was also utilized. Participants were included if they were administered both the MoCA and …

Marital Satisfaction, Parental Stress, & Perceived Social Support Of Mothers Of Children With Autism Spectrum Disorders, Miriam Khiari Millan

Theses and Dissertations

The present study investigated the effects of both formal and informal social supports on mothers of children with autism spectrum disorder’s marital satisfaction and parental stress. To our knowledge, no other study has examined the relationship between various formal support types and marital satisfaction. This study aimed to fill this gap by examining the effects of both formal and informal social supports on mothers of children with ASD’s marital satisfaction and maternal stress. A sample of 151 mothers of children with ASD completed the online survey, assessing their use of formal and informal supports, their perceived effectiveness of said social …

Decitizenizing Asian Pacific American Women, Shoba Sivaprasad Wadhia, Margaret Hu

Faculty Publications

The Page Act of 1875 excluded Asian women immigrants from entering the United States, presuming they were prostitutes. This presumption was tragically replicated in the 2021 Atlanta Massacre of six Asian and Asian American women, reinforcing the same harmful prejudices. This Article seeks to illuminate how the Atlanta Massacre is symbolic of larger forms of discrimination, including the harms of decitizenship. These harms include limited access to full citizenship rights due to legal barriers, restricted cultural and political power, and a lack of belonging. The Article concludes that these harms result from the structure of past and present immigration laws …

Assessing The Causal Effects Of Adipokines On Uric Acid And Gout: A Two-Sample Mendelian Randomization Study, Ruyi Cong, Xiaoyu Zhang, Zihong Song, Shanshan Chen, Guanhua Liu, Yizhi Liu, Xiuyu Pang, Fang Dong, Weijia Xing, Youxin Wang, Xizhu Xu

Research outputs 2022 to 2026

Previous observational studies have highlighted associations between adipokines and hyperuricemia, as well as gout, but the causality and direction of these associations are not clear. Therefore, we attempted to assess whether there are causal effects of specific adipokines (such as adiponectin (ADP) and soluble leptin receptors (sOB-R)) on uric acid (UA) or gout in a two-sample Mendelian randomization (MR) analysis, based on summary statistics from large genome-wide association studies. The inverse-variance weighted (IVW) method was performed as the primary analysis. Sensitivity analyses (including MR-Egger regression, weighted median, penalized weighted median, and MR pleiotropy residual sum and outlier methods) were also …

Evaluation Of Pd-L1 Expression On Circulating Tumour Cells In Small-Cell Lung Cancer, Emmanuel Acheampong, Afaf Abed, Michael Morici, Isaacs Spencer, Aaron B. Beasley, Samantha Bowyer, Du Bois Asante, Chris Lomma, Weitao Lin, Michael Millward, Elin S. Gray

Research outputs 2022 to 2026

Background: Antibodies against the programmed death-1 (PD-1) receptor and its ligand (PD-L1) have been recently approved for small-cell lung cancer (SCLC) treatment. Circulating tumour cells (CTCs) have emerged as an appealing liquid biopsy candidate that could enhance treatment decision-making in systemic therapy for SCLC patients. Several current technologies enrich CTCs using specific surface epitopes, size, rigidity, or dielectric properties. However, they are hampered by the heterogeneity of the enriched cells from blood samples. Methods: We evaluated two CTC enrichment systems: EpCAM conjugated to magnetic beads and a microfluidic device (Parsortix, Angle plc). PD-L1 expression was evaluated on the isolated CTCs. …

Enhanced Immunoprecipitation Techniques For The Identification Of Rna-Binding Protein Partners: Igf2bp1 Interactions In Mammary Epithelial Cells, Saja A Fakhraldeen, Scott M Berry, David J Beebe, Avtar Roopra, Celia M Bisbach, Vladimir S Spiegelman, Natalie M Niemi, Caroline M Alexander

2020-Current year OA Pubs

RNA-binding proteins (RBPs) regulate the expression of large cohorts of RNA species to produce programmatic changes in cellular phenotypes. To describe the function of RBPs within a cell, it is key to identify their mRNA-binding partners. This is often done by crosslinking nucleic acids to RBPs, followed by chemical release of the nucleic acid fragments for analysis. However, this methodology is lengthy, which involves complex processing with attendant sample losses, thus large amounts of starting materials and prone to artifacts. To evaluate potential alternative technologies, we tested "exclusion-based" purification of immunoprecipitates (IFAST or SLIDE) and report here that these methods …

Pharmacological Chaperones For The Oxytocin Receptor Increase Oxytocin Responsiveness In Myometrial Cells, Manasi Malik, Yingye Fang, Monali Wakle-Prabagaran, Michelle Roh, Kevin Prifti, Antonina I Frolova, Princess I Imoukhuede, Sarah K England

2020-Current year OA Pubs

Oxytocin is a potent uterotonic agent administered to nearly all patients during childbirth in the United States. Inadequate oxytocin response can necessitate Cesarean delivery or lead to uterine atony and postpartum hemorrhage. Thus, it may be clinically useful to identify patients at risk for poor oxytocin response and develop strategies to sensitize the uterus to oxytocin. Previously, we showed that the V281M variant in the oxytocin receptor (OXTR) gene impairs OXTR trafficking to the cell surface, leading to a decreased oxytocin response in cells. Here, we sought to identify pharmacological chaperones that increased oxytocin response in cells expressing WT or …

Phylogenetic And Expression Studies Of Small Gtp-Binding Proteins In Solanum Lycopersicum Super Strain B, Hassan S. Al-Zahrani, Tarek A.A. Moussa, Hameed Alsamadany, Rehab M. Hafez, Michael P. Fuller

School of Biological and Marine Sciences

This investigation involved a comparative analysis of the small GTPase superfamily in S. lycopersicum super strain B compared to their analogues in leguminous and other non-leguminous species. The small GTPases superfamily members were recognized by tBLASTn searches. The sequences of amino acid were aligned using Clustal Omega and the analysis of phylogeny was performed with the MEGA7 package. Protein alignments were applied for all studied species. Three-dimensional models of RABA2, ROP9, and ROP10 from Solanum lycopersicum “Super strain B” were performed. The levels of mRNA of the Rab, Arf, Rop, and Ran subfamilies were detected in aerial tissues vs. roots. …

In Vivo Ps-Oct Needle Probe Scan Of Human Skeletal Muscle, Jeffrey M. Mcbride, Michael J. Hackmann, Sophia Nimphius, Barry Cense

Research outputs 2022 to 2026

Polarization-sensitive optical coherence tomography (PS-OCT) derived birefringence values effectively identify skeletal muscle structural disruption due to muscular dystrophy and exercise-related muscle damage in animal models in ex vivo tissue. The purpose of this investigation was to determine if a PS-OCT needle probe inserted into the leg of a human subject could accurately identify various anatomical structures with implications for use as a diagnostic tool for the determination of skeletal muscle pathology. A healthy middle-aged subject participated in this study. A custom-built PS-OCT system was interfaced with a side-viewing fiber-optic needle probe inserted into the subject's vastus lateralis muscle via a …

Alcohol Consumption And Cardiovascular Outcomes In Patients With Nonalcoholic Fatty Liver Disease: A Population-Based Cohort Study, Malik Janjua, Matthew Knuiman, Mark Divitini, Brendan Mcquillan, John K. Olynyk, Gary P. Jeffrey, Leon A. Adams

Research outputs 2022 to 2026

Low-level alcohol consumption is associated with reduced cardiovascular disease (CVD) in the general population. It is unclear whether this association is seen in patients with nonalcoholic fatty liver disease (NAFLD) who have an increased risk of CVD. We examined the association between alcohol consumption and CVD-related outcomes in subjects with NAFLD from a general population cohort. Subjects participating in the 1994-1995 Busselton Health survey underwent clinical and biochemical assessment. NAFLD was identified using the Fatty Liver Index of >60, and alcohol consumption quantified using a validated questionnaire. CVD hospitalizations and death during the ensuing 20 years were ascertained using the …

Development Of 1,2,4-Oxadiazole Antimicrobial Agents To Treat Enteric Pathogens Within The Gastrointestinal Tract, Noel P. Pitcher, Jitendra R. Harjani, Yichao Zhao, Jianwen Jin, Daniel Knight, Lucy Li, Papanin Putsathit, Thomas V. Riley, Glen P. Carter, Jonathan B. Baell

Research outputs 2022 to 2026

Colonization of the gastrointestinal (GI) tract with pathogenic bacteria is an important risk factor for the development of certain potentially severe and life-threatening healthcare-associated infections, yet efforts to develop effective decolonization agents have been largely unsuccessful thus far. Herein, we report modification of the 1,2,4-oxadiazole class of antimicrobial compounds with poorly permeable functional groups in order to target bacterial pathogens within the GI tract. We have identified that the quaternary ammonium functionality of analogue 26a results in complete impermeability in Caco-2 cell monolayers while retaining activity against GI pathogens Clostridioides difficile and multidrug-resistant (MDR) Enterococcus faecium. Low compound recovery levels …

High Definition Tdcs Effect On Postural Control In Healthy Individuals: Entropy Analysis Of A Crossover Clinical Trial, Diandra B. Favoretto, Eduardo Bergonzoni, Diego Carvalho Nascimento, Francisco Louzada, Tenysson W. Lemos, Rosangela A. Batistela, Renato Moraes, João P. Leite, Brunna P. Rimoli, Dylan J. Edwards, Taiza G.S. Edwards

Research outputs 2022 to 2026

Objective: Converging evidence supporting an effect of transcranial direct current stimulation (tDCS) on postural control and human verticality perception highlights this strategy as promising for post-stroke rehabilitation. We have previously demonstrated polarity-dependent effects of high-definition tDCS (HD-tDCS) on weight-bearing asymmetry. However, there is no investigation regarding the time-course of effects on postural control induced by HD-tDCS protocols. Thus, we performed a nonlinear time series analysis focusing on the entropy of the ground reaction force as a secondary investigation of our randomized, double-blind, placebo-controlled, crossover clinical trial. Materials and Methods: Twenty healthy right-handed young adults received the following conditions (random order, …

Travel Ban Effects On Sars-Cov-2 Transmission Lineages In The Uae As Inferred By Genomic Epidemiology, Andreas Henschel, Samuel F. Feng, Rifat A. Hamoudi, Gihan Daw Elbait, Ernesto Damiani, Fathimathuz Waasia, Guan K. Tay, Bassam H. Mahboub, Maimunah Hemayet Uddin, Juan Acuna, Eman Alefishat, Rabih Halwani, Herbert F. Jelinek, Farah Mustafa, Nawal Alkaabi, Habiba S. Alsafar

Research outputs 2022 to 2026

Global and local whole genome sequencing of SARS-CoV-2 enables the tracing of domestic and international transmissions. We sequenced Viral RNA from 37 sampled Covid-19 patients with RT-PCR-confirmed infections across the UAE and developed time-resolved phylogenies with 69 local and 3,894 global genome sequences. Furthermore, we investigated specific clades associated with the UAE cohort and, their global diversity, introduction events and inferred domestic and international virus transmissions between January and June 2020. The study comprehensively characterized the genomic aspects of the virus and its spread within the UAE and identified that the prevalence shift of the D614G mutation was due to …

Electro‐Driven Materials And Processes For Lithium Recovery—A Review, Anna Siekierka, Marek Bryjak, Amir Razmjou, Wojciech Kujawski, Aleksandar N. Nikoloski, Ludovic F. Dumée

Research outputs 2022 to 2026

The mass production of lithium‐ion batteries and lithium‐rich e‐products that are required for electric vehicles, energy storage devices, and cloud‐connected electronics is driving an unprecedented demand for lithium resources. Current lithium production technologies, in which extraction and purification are typically achieved by hydrometallurgical routes, possess strong environmental impact but are also energy‐intensive and require extensive operational capabilities. The emergence of selective membrane materials and associated electro‐processes offers an avenue to reduce these energy and cost penalties and create more sustainable lithium production approaches. In this review, lithium recovery technologies are discussed considering the origin of the lithium, which can be …

Students’ Engagement In Education As Sustainability: Implementing An Ethical Dilemma-Steam Teaching Model In Chemistry Learning, Yuli Rahmawati, Elisabeth Taylor, Peter Charles Taylor, Achmad Ridwan, Alin Mardiah

Research outputs 2022 to 2026

This paper reports the results of a study on the implementation of the Ethical Dilemma STEAM Teaching Model in secondary schools in Jakarta, Indonesia. This interdisciplinary curriculum approach employed ‘ethical dilemma story pedagogy’ in a STEAM education project designed to engage students in values-based chemistry learning. Drawing on the arts, specially written ethical dilemma stories posing real-world environmental problems engaged students in exploring their value systems. Students reflected on the pros and cons of ethical dilemmas related to the everyday use of artificial fertilizers, disposal of used cooking oil and detergent waste, and environmental pollution caused by plastic waste. The …

The Interactive Effects Of Temperature And Nutrients On A Spring Phytoplankton Community, Stephanie I. Anderson, Gayantonia Franzè, Joshua D. Kling, Paul Wilburn, Colin T. Kremer, Susanne Menden-Deuer, Elena Litchman, David A. Hutchins, Tatiana A. Rynearson

Graduate School of Oceanography Faculty Publications

A complex interplay of environmental variables impacts phytoplankton community composition and physiology. Temperature and nutrient availability are two principal factors driving phytoplankton growth and composition, but are often investigated independently and on individual species in the laboratory. To assess the individual and interactive effects of temperature and nutrient concentration on phytoplankton community composition and physiology, we altered both the thermal and nutrient conditions of a cold-adapted spring phytoplankton community in Narragansett Bay, Rhode Island, when surface temperature was 2.6°C and chlorophyll > 9 μg L−1. Water was incubated in triplicate at −0.5°C, 2.6°C, and 6°C for 10 d. At each temperature, …

Parent-Of-Origin Effects Propagate Through Networks To Shape Metabolic Traits, Juan F. Macias-Velasco, Celine L.St Pierre, Jessica P. Wayhart, Li Yin, Larry Spears, Mario A. Miranda, Caryn Carson, Katsuhiko Funai, James M. Cheverud, Clay F. Semenkovich, Heather A. Lawson

Biology: Faculty Publications and Other Works

Parent-of-origin effects are unexpectedly common in complex traits, including metabolic and neurological traits. Parent-of-origin effects can be modified by the environment, but the architecture of these gene-by-environmental effects on phenotypes remains to be unraveled. Previously, quantitative trait loci (QTL) showing context-specific parent-of-origin effects on metabolic traits were mapped in the F16 generation of an advanced intercross between LG/J and SM/J inbred mice. However, these QTL were not enriched for known imprinted genes, suggesting another mechanism is needed to explain these parent-of-origin effects phenomena. We propose that non-imprinted genes can generate complex parent-of-origin effects on metabolic traits through interactions with imprinted …

Conserving The American Man: Gender, Eugenics And Education In The Civilian Conservation Corps, Charles Tocci, Ann Marie Ryan

Education: School of Education Faculty Publications and Other Works

The Civilian Conservation Corps (CCC) was a novel United States federal education programme that enrolled nearly three million men during the 1930s and early 1940s. This public work relief programme provides a case study of the ways that masculine, eugenicist ideas concerning public education evolved from the Progressive Era through the Great Depression. This educational philosophy was espoused by a small group of men – some educators, some not – who sought to remedy what they saw as the failures of public schooling, namely its overly feminine nature. Through an analysis of their public writings and the images that were …