Digital Commons Network^™

Evolution Of The Repression Mechanisms In Circadian Clocks, Jonathan Tyler, Yining Lu, Jay Dunlap, Daniel B. Forger

Dartmouth Scholarship

Background: Circadian (daily) timekeeping is essential to the survival of many organisms. An integral part of all circadian timekeeping systems is negative feedback between an activator and repressor. However, the role of this feedback varies widely between lower and higher organisms. Results: Here, we study repression mechanisms in the cyanobacterial and eukaryotic clocks through mathematical modeling and systems analysis. We find a common mathematical model that describes the mechanism by which organisms generate rhythms; however, transcription’s role in this has diverged. In cyanobacteria, protein sequestration and phosphorylation generate and regulate rhythms while transcription regulation keeps proteins in proper stoichiometric balance. …

Establishing The Efficacy Of Non-Cellular Components Of Adipose-Derived Stromal Vascular Fraction In Promoting Angiogenesis., Daniel Benson

Electronic Theses and Dissertations

Microvascular disease is hallmarked by pathophysiological conditions such as endothelial senescence, intimal thickening which impairs vasodilation, and regression of the capillary beds causing tissue ischemia in the myocardium or in peripheral vascular networks. Adipose-derived stromal vascular fraction (SVF) has previously demonstrated the ability to revascularize tissue. Increasing evidence shows that regenerative cells elicit their therapeutic benefit by paracrine mechanisms, leaving open extracellular vesicles (EVs) as a potential crux of the cell therapy paradigm. To test this idea, three types of gelatin methacrylate hydrogels were employed: SVF gels, EV gels derived from SVF, and blank control gels, which were used in-vitro …

Computational Fluid Dynamics Modeling Of Hemodialysis In Patients With An Arteriovenous Fistula, Maximilian Roth

McKelvey School of Engineering Graduate Student Theses & Dissertations

With the advent of arteriovenous fistula (AVF) for use in hemodialysis, the anastomosis built for such use has become a central point of the study to understand the flow and wall shear stresses in such a system since very large wall shear stresses can lead to arterial/vein rupture. Considering the commonly used creation site of an anastomosis as connecting the radial artery to the cephalic vein, a model is created to calculate the wall shear stresses across various components of the system. The model depicts a connection of the specified vein and artery bridged together allowing the increase in blood …

Prevalence Of Non-Self Dna On Three Different Sebaceous Skin Locations, Jan Kirstine Zamora

Student Theses

This project serves to provide additional information on the prevalence of non-self DNA on the exposed skin locations specifically the inner upper arm, the nape area of the neck and the neck area just below the ear, where current literature is lacking. Skin tape lifts from these locations were collected from 15 male participants and 13 female participants once a week over a period of three weeks. Only single source profiles were obtained from six volunteers. Eight volunteers had only one mixture DNA profile in nine samples, while mixture DNA profiles for at least two collection occasions were observed in …

The Intersection Of Religion And Mental Health Help-Seeking: Themes Within Youth Experiencing Early Psychosis, Breanna Nichols

Student Theses

Little research has examined the intersection of religion and mental health among predominantly conservative communities – where religion tends to weigh heavily. It is known from the literature that religion and spirituality play a role in influencing treatment pathways and views towards mental health. The primary aim of the present study was to explore via secondary thematic analysis, the intersection of religion and mental health within a conservative Midwestern community of youth who are receiving treatment for early psychosis, with a secondary look at family dynamics. Seven participant transcripts were analyzed from the Narrative Enhancement and Cognitive Therapy-Young Adult (NECT-YA) …

Linking Homelessness In Secondary School To Postsecondary And Early Labor Market Outcomes In Maryland Using A Continuum Of Risk Framework, Dawnsha R. Mushongaa, Mathew C. Uretsky, Bess A. Rose, Angela K. Henneberger

School of Social Work Faculty Publications and Presentations

Homeless and low-income students share multiple overlapping risk experiences; however, some studies report little to no observed differences in outcomes between these students. From the cumulative risk perspective, homelessness is perched at the extreme edge of economic insecurity, suggesting that homeless students encounter additional hardships beyond poverty experiences alone. Using a continuum of risk framework, this study leveraged statewide administrative data from a 6th grade cohort of Maryland public school students (N = 52,610) to investigate the association between homelessness and educational and early labor market outcomes, above and beyond the associations linked to poverty. Findings provide support for the …

Landscape Genomics Of The Tussock Cottongrass (Eriophorum Vaginatum) And The Dwarf Birch (Betula Nana) In North Central Alaska, Elizabeth Stunz

Open Access Theses & Dissertations

Global climate change has resulted in geographic range shifts of flora and fauna at a global scale. Extreme environments, like the Arctic, are seeing some of the most pronounced changes. This region covers 14% of the Earthâ??s land area, and while many arctic species are widespread, understanding ecotypic variation at the genomic level will be important for elucidating how range shifts will affect ecological processes. Increase in shrub cover is a major effect of ongoing climate change in arctic tundra ecosystems. The relative increases in abundance and cover of shrub species such as birch, willow, and alder (Betula, Salix, and …

Acute Effects Of Neuromuscular Electrical Stimulation On Blood Glucose And Energy Metabolism In Overweight/Obese Population, Ali Mossayebi

Open Access Theses & Dissertations

Background: In the perspective of preventing cardiovascular disease and promoting health, weight control and physical activity are the main concerns. Electrical stimulation (ES) is an alternate strategy to induce muscle contraction, using electrical impulses. However, effectiveness of ES induced muscle contraction to improve energy expenditure and glucose metabolism is not well examined.Purpose: To determine the acute effect of ES on whole body energy expenditure and body glucose metabolism in sedentary overweight or obese population Methods: Sedentary overweight/obese participants (n=8; age: 37.25 ± 6.09 years; BMI= 37.57 ± 2.43 kg/m2) were included in this study. All participants received bilateral quadriceps ES …

Lgd-4033 And Mk-677 Use Impacts Body Composition, Circulating Biomarkers, And Skeletal Muscle Androgenic Hormone And Receptor Content: A Case Report, Thomas D. Cardaci, Steven B. Machek, Dylan T. Wilburn, Jeffery L. Heileson, Dillon R. Harris, Harry P. Cintineo, Darryn S. Willoughby

Kinesiology Faculty Publications and Presentations

LGD-4033, a selective androgen receptor modulator, and MK-677, a growth hormone secretagogue, are being used increasingly amongst recreationally active demographics. However, limited data exist describing their effects on health- and androgen-related biomarkers. The purpose of this case study was to determine changes in body composition and biomarkers during and after continued co-administration of LGD-4033 and MK-677. We also aimed to examine muscular strength and intramuscular androgen-associated biomarkers relative to non-users. A 25-year-old male ingested LGD-4033 (10 mg) and MK-677 (15 mg) daily for 5 weeks. Blood and body composition metrics were obtained pre-, on- and post-cycle. One-repetition maximum leg and …

Effect Of Gut Microbiota On Colorectal Cancer Progression And Treatment, Glowi A. Alasiri

Saudi Medical Journal

Microbiota is a collection of bacteria, archaea, eukaryotes, bacteriophages, viruses, and fungi that cover human body surfaces and cavities. They characterize inside the body due to several factors such as diet, nutrition, xenobiotic substances, and microbial infections. Several studies have shown that gut microbiota can induce resistance against pathogens and regulate the immune system. In addition, their disruption is associated with several physiological and biochemical disorders, including inflammatory bowel disease (IBD), obesity, autoimmune diseases such as diabetes, hypertension, colon cancer, and cardiovascular disease. Colorectal cancer (CRC) is the third-deadliest cancer worldwide, accounting for approximately 900,000 deaths per year globally. Gut …

Clinical, Laboratory, And Chest Radiographic Characteristics Of Covid-19 Associated Severe Pediatric Pneumonia. A Retrospective Study, Ali A. Asseri, Saleh M. Al-Qahtani, Mohammed A. Algathradi, Ibrahim A. Alzaydani, Ahmed A. Al-Jarie, Ibrahim A. Al-Benhassan, Ibrahim A. Alhelali, Mona S. Alassiri, Ali A. Alrmelawi, Abdelwahid S. Ali

Saudi Medical Journal

Objectives: To evaluate the demographics, clinical presentation, laboratory data, chest radiographs, and outcomes of pediatric patients with critical coronavirus disease 2019 (COVID-19). Methods: This retrospective study included 34 children who were diagnosed with severe COVID-19 pneumonia between August 2020 and July 2021. Severe pneumonia was defined as fever, respiratory distress (tachypnea, chest retractions, and hypoxia [oxygen saturation <90% in room air]), and obvious infiltrations on chest radiography. Results: Ages of the patients ranged from newborns to 12 years old, with a median of 24 months (interquartile range: 12-72 months). Preschool-aged children were the most common age group (44%). Levels of inflammatory markers (C-reactive protein, ferritin, and procalcitonin) were elevated in most patients. A total of 13 patients developed severe acute respiratory distress syndrome (ARDS), while 4 developed multiorgan failure. Despite receiving supportive therapy, 2 (5.9%) patients died due to severe septic shock and multiorgan failure. One deceased patient was born prematurely at 30 weeks, while the other had chronic granulomatous disease. Conclusion: This study described a single-center cohort of pediatric patients with severe COVID-19 pneumonia. In this cohort, children with cardiopulmonary comorbidities and ARDS had a high mortality and long-term morbidity, as observed in other pediatric studies.

Exploring The Anticancer Mechanism Of Thienopyrazole Derivative Tpz-1 In Acute Myeloid Leukemia, Jessica Dyanne Hess

Open Access Theses & Dissertations

Anticancer drug discovery is a time and resource-consuming process for which exceedingly reliable and efficient modern approaches are needed. Phenotypic drug screenings can generate highly potent and innovative drug candidates; however, deconvolution of the drugâ??s target often presents significant barriers to drug development. To overcome this hurdle, we have originally combined in vitro and in silico analyses to uncover the molecular mechanism(s) driving the anticancer activity of the uniquely structured small molecule drug candidate, Tpz-1. Our study revealed that Tpz-1 is a multitargeted agent which induces the programmed death of HL-60 acute myeloid leukemia cells primarily through disruption of microtubule …

Implementation Of Structure From Motion (Sfm) Technology For Edcuational Lessons, Valeria Veronica Martinez

Open Access Theses & Dissertations

Education is the most important obligation the humanity race has in order to evolve and thrive in the modern world. There are various applications for educators to teach valuable lessons to students, and not all deliveries will be the same for every lesson and for every student. Lesson being taught, the lesson delivery from educator to student often changes every time the lesson is implemented, depending on the learning ability of the students. Students have the ability to learn in different ways, such as the visual, auditory, kinesthetic, reading and writing learners (Oxford 2003). As educators aware of the four …

Spectroscopic Study Of Bi5ti3feo15 Aurivillius Compound For Multifunctional Applications, Mariana Castellanos

Open Access Theses & Dissertations

No abstract provided.

Effects Of Eccentric Cycling On Blood Flow Patterns And Vascular Reactivity, Manuel Gomez

Open Access Theses & Dissertations

Decrease of blood flow to tissues, due to atherosclerotic plaque formation, produces mortality and morbidity outcomes. Vascular homeostasis depends in the direct interaction between blood flow and endothelial cells, known as endothelial sheer stress (ESS). In addition, turbulent flow, which can be measured by Reynolds number (Re), has been associated with decrease ESS. Eccentric exercise (ECC) has gained attention as a novel exercise modality that increases muscle performance without increasing metabolic demands; however, little is known about ESS during ECC. PURPOSE: To determine the effects of ECC cycling in blood flow patterns and vascular reactivity in young, healthy individuals. METHODS: …

Empowering The Neurodiverse Community Through Sex Education, Talya Mainzer

Capstone Projects and Master's Theses

The Without Walls (WOW) Program is a collaboration between Gateway Center of Monterey County and Pacific Grove Adult Education, serving neurodiverse adults between the ages of 18-55. Historically, people with disabilities have been excluded from comprehensive social-emotional and sexual health education which was reflected in the findings of a survey distributed to participants of the WOW program. Of the respondents, 71.4 percent reported never receiving sex education while 57.1 percent reported never receiving education regarding healthy relationships. This is problematic as people with disabilities are more likely to experience harm and are less likely to report it compared to their …

Inspiring Students In Careers In Science, Technology, Engineering, And Math (Stem) Using Science Fairs, Sydney Ann Guire

Capstone Projects and Master's Theses

Science fairs have many benefits when it comes to students’ academic success. They are an opportunity for them to explore, experiment, and find their own interests. Science fairs help provide students with experiences and pursue future careers in STEM. Given the importance in STEM, not all school districts host science fairs for their students. The purpose of this capstone project is to examine the benefits of science fairs on students through the use of literature review, teacher and school administrator interviews, and student surveys. The findings indicate that science fairs inspire students to create a love for science and continue …

Teaching Immigrant Parents About Mental Health, Ana Rodriguez

Capstone Projects and Master's Theses

Teaching immigrant parents about mental health awareness is vital for the Latino community because of the lack and high barriers for them to obtain resources on mental health. The immigrant parents have a background in English studies at the Watsonville adult school and Cabrillo College in Watsonville, California. The immigrant parents are from Mexico, and they are from different states in Mexico. Many immigrant parents have language barriers that prevent them from communicating and accessing mental health resources in the community to support them. Cultural competency can affect the misdiagnosis of mental health illnesses and education. Additionally, immigrant parents' status …

Coping Strategies To Decrease Ptsd For Previously Incarcerated Veterans, Simone The

Capstone Projects and Master's Theses

The focal topic of this capstone project is the emotional well-being and improvement of formerly incarcerated veterans who were transitioning out of the California prison system. The median age of formerly incarcerated age in Washington state in 2018 was 48.3 years old (Drapela, 2018). A focus on emotional well-being for this demographic is needed due to the individuals being at a higher risk of suicide, homelessness, and mental illness (Spence, Crawford, & LePage, 2020). Previously incarcerated veterans often face mental illness from experiences of high stress and traumatic situations within prison, the military, and events from their childhood. To attempt …

Effects Of Sediment Addition On Macroinvertebrate Communities In New England Salt Marshes, Chloe Brownlie

Master's Theses and Capstones

Salt marsh invertebrates play a vital role in salt marsh ecosystems by aerating the marsh, cycling nutrients, and forming connections between trophic levels, but the impacts of large-scale natural and anthropogenic sediment addition on their community structure and species composition is poorly understood. During winter storm Grayson in January of 2018, ice rafting caused large areas of sediment to deposit on significant areas of the Great Marsh in Massachusetts. I hypothesized that sediment addition would influence the abundance and richness of invertebrates in the marsh, because they tend to take years to recover after a disturbance. Sediment cores were taken …

Investigating The Role Of Palmitoyl Acyltransferase 14 In Preventing Early Death In Arabidopsis Thaliana, Robert Elliott

Master's Theses and Capstones

Palmitoylation is a reversible post-translational modification that affects the location, activity, and stability of proteins. Arabidopsis thaliana contains 23 genes for palmitoyl acyltransferases (PATs), the enzymes that catalyze palmitoylation. Loss of PAT14 activity results in early senescence and diminished plant size when plants are grown on standard peat/perlite mixtures in pots and watered with a commercial fertilizer, but this phenotype is not observed when plants grown on agar plates containing a different nutrient solution. Here we show that neither the peat/perlite growth medium nor the commensal microbes present in the growth are the causative agents for phenotype development. However, the …

The Cowl - V. 87 N. 8 - December 1, 2022

The Cowl

The Cowl - student newspaper of Providence College. Vol. 87 No. 8 - December 1, 2022. 16 pages.

Predicting Enrollment In A Metropolitan University In Southeast Tennessee, Cynthia Williamson

Masters Theses and Doctoral Dissertations

Institutions of higher education are tasked with making decisions that will impact their students, faculty, staff, and other stakeholders. Many of these decisions are focused on varying aspects of budgeting, and an institution’s sustainability and reputation could be impacted by misallocations of resources. Some of this risk could potentially be eliminated if there was a strategic way to predict which data points have meaningful impact on student enrollment. This study used data collected at a metropolitan university in Southeast Tennessee for six fall semesters to develop enrollment prediction models for the institution. The focus of the study was to determine …

The Lack Of Natural Igm Increases Susceptibility And Impairs Anti-Vi Polysaccharide Igg Responses In A Mouse Model Of Typhoid, Akhil S. Alugupalli, Matthew P. Cravens, Justin A. Walker, Dania Gulandijany, Gregory S. Dickinson, Genevieve Lewis, Gudrun F. Debes, Dieter M. Schifferli, Andreas J. Bäumler, Kishore R. Alugupalli

Department of Microbiology and Immunology Faculty Papers

Circulating IgM present in the body prior to any apparent Ag exposure is referred to as natural IgM. Natural IgM provides protective immunity against a variety of pathogens. Salmonella enterica serovar Typhi (S. Typhi) is the causative agent of typhoid fever in humans. Because mice are not permissive to S. Typhi infection, we employed a murine model of typhoid using S. enterica serovar Typhimurium expressing the Vi polysaccharide (ViPS) of S. Typhi (S. Typhimurium strain RC60) to evaluate the role of natural IgM in pathogenesis. We found that natural mouse IgM binds to S. Typhi and S. Typhimurium. The severity …

Uncloaking Cell-Impermeant Gold Nanorods Via Tumor Microenvironmental Cathepsin B Facilitates Cancer Cell Penetration And Potent Radiosensitization, Subhiksha Raghuram, Yuri Mackeyev, Jessica Symons, Yasmin Zahra, Valeria Gonzalez, Krishnan K Mahadevan, Katherinne I Requejo, Anton Liopo, Paul Derry, Eugene Zubarev, Onur Sahin, Joseph Byung-Kyu Kim, Pankaj K Singh, Sang Hyun Cho, Sunil Krishnan

Faculty, Staff and Student Publications

Major impediments to conveyance of intravenously administered drugs to tumors are biofouling, opsonization, and rapid clearance from the circulation by macrophages and reticuloendothelial phagocytes. Cloaking nanoparticles with stealth epilayers partly overcomes these hurdles but it also foils interactions with tumor cells. Here, we describe the synthesis, characterization, and validation of smart gold nanorods (GNRs) that spontaneously transform from inert passengers in the blood stream to active cell-penetrating nanoparticles within tumors to potently sensitize tumors to radiation therapy. Intrinsically cationic and cell-penetrating GNRs were shielded from phagocytosis with a cloaking polyethylene glycol epilayer containing an intervening cleavable peptide. In the absence …

Metabolic Changes Associated With Gestational Diabetes And Weight Loss, Marykate Smith

Master's Theses

Introduction: Gestational Diabetes Mellitus (GDM) is diabetes diagnosed for the first time during pregnancy and negatively affects the mother and her child. Research suggests a multidisciplinary lifestyle intervention may aid in the reduction of GDM and its complications. Further, the use of metabolomics is needed to understand the alterations that happen prior to GDM diagnosis to reduce its recurrence.

Objective: Metabolomics analysis was conducted at baseline and 16-weeks post-intervention for women enrolled in a pre-pregnancy lifestyle intervention study to observe metabolic changes associated with GDM occurrence and weight loss.

Methodology: Sixteen samples from the parent Gestational Diabetes Prevention trial were …

Dynamic Soil Properties In The Lower Rio Grande Valley: Understanding Responses Of Infiltration Rate, Bulk Density, Aggregate Stability To Different Tillage Practices, Temiloluwa Faith Awotoye

Theses and Dissertations

Dynamic Soil Properties (DSPs) are soil properties that change in response to both natural and human-caused interruptions and pressures such as agro-based and wildland management. These soil properties can transform for months or even years in response to the farm use and management procedures. This research project investigates the relationship between different dynamic soil physical properties and tillage practices in the Texas Hidalgo sandy clay loam benchmark soil series. Data analyses was done to compare the effect of tillage to these soil physical properties and build a discussion about relationships between on-farm practices to other aspects of farm viability. We …

Dynamic Soil Properties In The Lower Rio Grande Valley: Carbon And Nitrogen Responses To Different Tillage Practices, Luzyannet Ballesteros Gonzalez

Theses and Dissertations

There has been increased attention in utilizing agriculture for carbon sequestration. Soil carbon and nitrogen are two dynamic soil properties (DSPs) that are indicators of soil health and function and have overlapping cycles. These soil properties change frequently as a result of environmental conditions and agricultural management practices. This study focuses on the impact of tillage practices in unirrigated agricultural fields in Hidalgo and Willacy counties located in South Texas on Hidalgo Sandy Clay Loam; With a particular focus on Soil Total Carbon (TC), Soil Inorganic Carbon (SIC), Permanganate Oxidizable Carbon (POXC), Carbon and Nitrogen ratio (C:N), Soil Organic Matter …

Wildlife Road Mortality Patterns In South Texas And Survey Methodology Improvement, Bradley E. Beer

Theses and Dissertations

Mortalities of wildlife caused by collisions with vehicles along roads are increasing in prevalence, threatening the existence of various species and populations. A better understanding of how mortalities change in response to natural and anthropogenic variables and efficient methods of obtaining mortality data are essential to mitigating such mortalities. This thesis investigates several key elements to improving road mortality surveys in south Texas. First, it was found that road mortality survey counts did not change under a pandemic-related lockdown and that 2 mortality survey observers detect more mortalities than 1. Analysis of brown pelican groundings on Texas State Highway 48 …

The Molecular Evolution Of Insect Opsin Genes, Maria Jose Cardenas Muedano

Theses and Dissertations

Opsins are part of the superfamily of G-coupled receptor proteins and together with chromophores, are responsible for initiating the signal transduction cascade responsible for animal vision. Since opsins have a critical role on visual perception these genes are crucial for a myriad of adaptive traits and behaviors and should show diverse signs of natural selection at the molecular level. Understanding the evolution of opsins across the wide diversity of insect groups will allow us to better understand the evolutionary pressure on insect opsins and serve as a model for both other gene systems and other taxonomic groups of animals that …