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Activation Domains Can Decouple The Mean And Noise Of Gene Expression, Kaiser Loell, Yawei Wu, Max V Staller, Barak Cohen

2020-Current year OA Pubs

Regulatory mechanisms set a gene's average level of expression, but a gene's expression constantly fluctuates around that average. These stochastic fluctuations, or expression noise, play a role in cell-fate transitions, bet hedging in microbes, and the development of chemotherapeutic resistance in cancer. An outstanding question is what regulatory mechanisms contribute to noise. Here, we demonstrate that, for a fixed mean level of expression, strong activation domains (ADs) at low abundance produce high expression noise, while weak ADs at high abundance generate lower expression noise. We conclude that differences in noise can be explained by the interplay between a TF's nuclear …

Floc Size Distributions Of Cohesive Sediment In Homogeneous Isotropic Turbulence, M Yu, X Yu, S Balachandar, Aj Manning

School of Biological and Marine Sciences

Floc size distribution is one of the key parameters to characterize flocculating cohesive sediment. An Eulerian–Lagrangian framework has been implemented to study the flocculation dynamics of cohesive sediments in homogeneous isotropic turbulent flows. Fine cohesive sediment particles are modeled as the dispersed phase by the discrete element method, which tracks the motion of individual particles. An adhesive contact model with rolling friction is applied to simulate the particle–particle interactions. By varying the physicochemical properties (i.e., stickiness and stiffness) of the primary particles, the dependence of the mathematical form of the floc size distribution on sediment properties is investigated. At the …

Examining The Generalizability Of Research Findings From Archival Data, Andrew Delios, Elena Giulia Clemente, Tao Wu, Hongbin Tan, Yong Wang, Michael Gordon, Domenico Viganola, Z Chen, Anna Dreber, Magnus Johannesson, Thomas Pfeiffer, Eric Luis Uhlmann, Al-Aziz Am Abd, Ajay T. Abraham, Jais Trojan, Matus Adamkovic, Elena Agadullina, Jungsoo Ahn, Cinla Akinci, Handan Akkas, David Albrecht, Shilaan Alzahawi, Marcio Amaral-Baptista, Rahul Anand, Kevin Francis U. Ang, Frederik Anseel, John Jamir Benzon R. Aruta, Mujeeba Ashraf, Bradley J. Baker, Xueqi Bao

School of Psychology

This initiative examined systematically the extent to which a large set of archival research findings generalizes across contexts. We repeated the key analyses for 29 original strategic management effects in the same context (direct reproduction) as well as in 52 novel time periods and geographies; 45% of the reproductions returned results matching the original reports together with 55% of tests in different spans of years and 40% of tests in novel geographies. Some original findings were associated with multiple new tests. Reproducibility was the best predictor of generalizability—for the findings that proved directly reproducible, 84% emerged in other available time …

Genetic And Environmental Variation Impact Transferability Of Polygenic Risk Scores, Daniel S. Araújo, Heather E. Wheeler

Biology: Faculty Publications and Other Works

Even when polygenic risk scores (PRSs) are trained in African ancestral populations, Kamiza and colleagues showed that genetic and environmental variation within sub-Saharan African populations impacts prediction performance, highlighting the challenges of clinical implementation of PRSs for risk assessment.

Exchange Broadening Underlies The Enhancement Of Ide-Dependent Degradation Of Insulin By Anionic Membranes, Qiuchen Zheng, Bethany Lee, Micheal T. Kebede, Valerie A. Ivancic, Merc M. Kemeh, Henrique Lemos Brito, Donald E. Spratt, Noel D. Lazo

Chemistry

Insulin-degrading enzyme (IDE) is an evolutionarily conserved ubiquitous zinc metalloprotease implicated in the efficient degradation of insulin monomer. However, IDE also degrades monomers of amyloidogenic peptides associated with disease, complicating the development of IDE inhibitors. In this work, we investigated the effects of the lipid composition of membranes on the IDE-dependent degradation of insulin. Kinetic analysis based on chromatography and insulin's helical circular dichroic signal showed that the presence of anionic lipids in membranes enhances IDE's activity toward insulin. Using NMR spectroscopy, we discovered that exchange broadening underlies the enhancement of IDE's activity. These findings, together with the adverse effects …

Magnetically Recoverable Silica-Decorated Ferromagnetic-Nanoceria Nanocatalysts And Their Use With O- And N-Butyloxycarbonylation Reaction Via Solvent-Free Condition, Shripad M Patil, Runjhun Tandon, Nitin Tandon

Faculty, Staff and Student Publications

Silica-decorated ferrite nanoparticles, a new kind, coated with ceric ammonium nitrate (CAN), have been prepared successfully by simple coprecipitation techniques. Powder X-ray diffraction spectroscopy (PXRD), Fourier transform-infrared spectroscopy (FT-IR), field emission-scanning electron microscope (FE-SEM), wavelength-dispersive X-ray spectroscopy (WDX), energy-dispersive spectroscopy (EDS), inductive coupled plasma-optical emission spectroscopy (ICP-OES), and thermogravimetric analysis (TGA) techniques were used to characterize these nanoparticles. The catalysts are further studied for catalytic activity in solvent-free conditions. Importantly, these nanoparticles have been collected from the reaction mixture using an external magnet and recycled up to minimum of 15 cycles with no substantial loss of catalytic characteristics.

The Process Of Establishing A Blooming Chemistry Tool For Use In Undergraduate Chemistry Education And Research, Emryse Geye

Dissertations and Theses

While it is still common in college chemistry to assess student learning and skill with summative assessments, the CER community does not currently have a simple tool to determine and communicate whether an assessment is actually aligned with the outcomes of interest. In particular, as so-called evidence-based teaching practices and active learning strategies gain a foothold in college chemistry classrooms, the ability to communicate whether those (often labor-, cost-, and time-intensive) interventions are not only aligned with course outcomes, but also provide measurable benefit to students becomes more imperative. While college chemistry has made some strides in the area of …

Parochial Altruism And Political Ideology, Marilynn B. Brewer, Nancy R. Buchan, Orgul D. Ozturk, Gianluca Grimalda

Faculty Publications

Parochial altruism refers to the propensity to direct prosocial behavior toward members of one's own ingroup to a greater extent than toward those outside one's group. Both theory and empirical research suggest that parochialism may be linked to political ideology, with conservatives more likely than liberals to exhibit ingroup bias in altruistic behavior. The present study, conducted in the United States and Italy, tested this relationship in the context of the COVID-19 pandemic, assessing willingness to contribute money to charities at different levels of inclusiveness—local versus national versus international. Results indicated that conservatives contributed less money overall and were more …

The Link Between Intracellular Calcium Signaling And The Nitric Oxide-Dependent Release Of Chloride In Retinal Amacrine Cells, Li Zhong

LSU Doctoral Dissertations

Regulation of cytosolic Cl^- is an important determinant in synaptic function. Our lab has discovered that cytosolic Cl^- is regulated not only by transport across the plasma membrane but that Cl^- can also be released from internal Cl^- stores (Hoffpauir et al., 2006) in amacrine cells (ACs). Nitric oxide (NO) is a small molecule neurotransmitter that has been demonstrated to be involved in cytosolic Ca²⁺ elevations and Cl^- regulation via activation of the cystic fibrosis transmembrane regulator (CFTR) (Krishnan et al., 2017). Here, I test the hypothesis that intracellular Ca²⁺ signaling mediates the …

Effect Of Race On Prediction Of Brain Amyloidosis By Plasma Aβ42/Aβ40, Phosphorylated Tau, And Neurofilament Light, Suzanne E Schindler, Rachel L Henson, Yan Li, Benjamin Saef, Krista L Moulder, David Bradford, Anne M Fagan, Brian A Gordon, Tammie L S Benzinger, Joyce Balls-Berry, Randall J Bateman, Chengjie Xiong, John C Morris, Et Al

2020-Current year OA Pubs

BACKGROUND AND OBJECTIVES: To evaluate whether plasma biomarkers of amyloid (Aβ42/Aβ40), tau (p-tau181 and p-tau231), and neuroaxonal injury (neurofilament light chain [NfL]) detect brain amyloidosis consistently across racial groups.

METHODS: Individuals enrolled in studies of memory and aging who self-identified as African American (AA) were matched 1:1 to self-identified non-Hispanic White (NHW) individuals by age,

RESULTS: There were 76 matched pairs of AA and NHW participants (n = 152 total). For both AA and NHW groups, the median age was 68.4 years, 42% were

DISCUSSION: Models predicting brain amyloidosis using a high-performance plasma Aβ42/Aβ40 assay may provide an accurate and …

Sox9 Directs Divergent Epigenomic States In Brain Tumor Subtypes, Debosmita Sardar, Hsiao-Chi Chen, Amanda Reyes, Srinidhi Varadharajan, Antrix Jain, Carrie Mohila, Rachel Curry, Brittney Lozzi, Kavitha Rajendran, Alexis Cervantes, Kwanha Yu, Ali Jalali, Ganesh Rao, Stephen C Mack, Benjamin Deneen

Duncan NRI Faculty and Staff Publications

Epigenetic dysregulation is a universal feature of cancer that results in altered patterns of gene expression that drive malignancy. Brain tumors exhibit subtype-specific epigenetic alterations; however, the molecular mechanisms responsible for these diverse epigenetic states remain unclear. Here, we show that the developmental transcription factor Sox9 differentially regulates epigenomic states in high-grade glioma (HGG) and ependymoma (EPN). Using our autochthonous mouse models, we found that Sox9 suppresses HGG growth and expands associated H3K27ac states, while promoting ZFTA-RELA (ZR

Hippo-Taz Signaling Is The Master Regulator Of The Onset Of Triple-Negative Basal-Like Breast Cancers, Hirotoshi Soyama, Miki Nishio, Junji Otani, Toshiko Sakuma, Shintaro Takao, Shigeo Hara, Takaaki Masuda, Koshi Mimori, Shinya Toyokuni, John P Lydon, Kazuwa Nakao, Hiroshi Nishina, Takumi Fukumoto, Tomohiko Maehama, Akira Suzuki

Faculty, Staff and Students Publications

A universal oncogenic driver of basal-like breast cancer (BLBC) has resisted identification. We show that continuous transcriptional coactivator with PDZ-binding motif (TAZ) activation in precancerous murine luminal cells generates luminal cancers that later become BLBCs. Subsequent TP53 alteration, a feature of invasive human BLBCs, accelerates tumor progression. Because BLBC development is inhibited by TAZ inactivation in vivo, our work provides a sound rationale for targeting Hippo-TAZ signaling as therapy for human BLBC. Our mouse model of BLBC represents a powerful tool for evaluating such drugs.

Blood Chemistry And Biliverdin Differ According To Reproduction And Tourism In A Free-Living Lizard, Susannah French

Browse all Datasets

Changes in the physiological health of species are an essential indicator of changing conditions and environmental challenges. Reponses to environmental challenges can often induce stress, influence physiology, and change metabolism in organisms. Here we tested blood chemistry parameters indicative of stress and metabolic activity using an i-STAT point-of-care blood analyzer in seven populations of free-ranging rock iguanas exposed to varying levels of tourism and supplemental feeding. We found significant differences in blood chemistry (glucose, oxygen, carbon dioxide, hematocrit, hemoglobin, calcium, potassium, and biliverdin levels) among populations exposed to varying levels of tourism, and also some variation between sexes and reproductive …

Box 06, Folder 26 - “The Montessori Method Of Education Syllabus Of Lectures” (E.M.S.), Edwin Mortimer Standing

Lecture Outlines, 1962-ca.1963; n.d., Edwin Mortimer Standing, ca.1963; n.d.

No abstract provided.

Effect Of In-Situ Modification Of Poly-Dopamine On Workability And Frost Resistance Of Glass Fiber Reinforced Cement Materials, Pengfei Ma

International Conference on Durability of Concrete Structures

No abstract provided.

Influence Of Biological Sulfuric Acid On Corrosion Mechanism Of Alkali-Activated Cement Concrete, 季 韬 教授

International Conference on Durability of Concrete Structures

生物硫酸对碱活化水泥混凝土腐蚀机理的影响

丁方硕、韬骥、潘伟杰

福州大学土木工程系

抽象的

讨论了不同浓度的生物硫酸对碱活化水泥混凝土（AACC）腐蚀的影响，并以化学硫酸为参考。AACC的质量和抗压强度、孔溶液的pH曲线和Ca ²⁺对其释放进行了研究，并通过 ESEM-EDS 和 XRD 揭示了 AACC 的腐蚀机理。结果表明，在不同浓度的化学硫酸和生物硫酸作用下，AACC的腐蚀产物主要是石膏。随着硫酸浓度的增加，AACC的失重率增加，AACC的抗压强度降低。石膏阻碍化学硫酸进入混凝土。然而，石膏为细菌提供了生存环境，细菌很容易渗透到混凝土中，加速了AACC的破坏。与化学硫酸相比，生物硫酸增加了AACC的质量损失率和抗压强度。

参考

荣H，Wu，C.，Ma，G.，Zheng，X.，和Liu，Z，2021。污水环境下混凝土微生物腐蚀研究进展。中国陶瓷学会学报，49（5）：988-999。

Scholz, F., 1997。微生物影响的材料腐蚀：科学和工程方面。固态电化学杂志，1（2）：181-181。

谢毅，林，X.，吉，T.，2019。普通硅酸盐混凝土与碱活化混凝土在生物硫酸侵蚀下的耐腐蚀机理比较。建筑和建筑材料，228（20）：1-12。

Enhancing Science Instruction Through The Use Of Graphic Organizers, Ellen G. Humphrey

LC Journal of Special Education

Just as a secondary social studies class requires students to meet a demanding curriculum and have a good deal of background knowledge, so does a secondary science class. Since research supports the use of graphic organizers, the purpose of this study is to investigate the impact of graphic organizers on content–area learning for high school students in a resource room for biology class where the teacher uses effective teaching practices. The study will specifically focus on improving science vocabulary, comprehension, and storing and recalling information by enhancing science instruction.

The Heart's "Little Brain"

Thomas Jefferson University Research Magazine

Researchers develop the first ever 3D map of the heart’s nervous system, providing a foundation for understanding the complexities of heart health.

A Journey Of Adaptation

Thomas Jefferson University Research Magazine

Consider Haloferax volcanii, a single celled organism that thrives in extreme conditions such as the very salty Dead Sea. Scientists study it for clues about potential life on Mars and about how organisms may adapt to Earth’s changing climate. Much like the organism she studies, H. volcanii researcher Manuela Tripepi, PhD, is an expert at adapting to challenging situations.

War Is The Storm—Clausewitz, Chaos, And Complex War Studies, B. A. Friedman

Naval War College Review

Complexity theories indeed do apply to war, and Clausewitz’s theories were the first to grapple with them. War is nonlinear, but the parallels between war and complexity science, and between complexity science and Clausewitz, do not stop with nonlinearity. Clausewitz’s theory of war does not just allude to complexity; rather, complexity is at its very core.

Bursts Of Power

Thomas Jefferson University Research Magazine

Mitochondria fire oxidants to communicate with organelles nearby, a communication that could be important in various diseases.

Building The Research Enterprise Of 2028, Mark L. Tykocinski, Md

Thomas Jefferson University Research Magazine

This edition of Jefferson Research offers a tantalizing peek at the array of work done at Thomas Jefferson University: from how mitochondria communicate with nearby organelles, to the use of innovative textiles to reduce a building’s energy consumption; from how “digital readiness” can improve health equity, to space travel’s effects on the immune system. It also serves as a lens on the exciting path that our university's research community will pursue over the next five years, guided by our new strategic plan for the Jefferson Research Enterprise

Reanalysis Of Teruelius And Grosphus (Scorpiones: Buthidae) With Descriptions Of Two New Species, Graeme Lowe, František Kovařík

Euscorpius

No abstract provided.

Using Scratch Wound Assay To Study The Effect Of Soil Arsenic On Human Keratinocyte Cell Migration Due To Contact Exposure, Manas Warke, Laura De March, Srinivas Kannan, Madeline English, Rohan Sarkar, Rupali Datta, Smitha Rao

Michigan Tech Research Data

The scratch wound assay was performed on Human immortalized keratinocytes (HaCaT) cells to observe the effect on cell migration due to contact exposure to arsenic-contaminated Immokalee soil. The cell migration was observed through a microscope for 72 h. HaCaT cells were seeded in 48-well plate. On day 3, treatment media was added (n=8). The cells were treated with four concentrations of soil As (45, 225, 450, and 900 mg/kg) and two controls - Negative control (NC; Pure media) and control (C; 0 mg/kg soil As) for 72 h. A scratch was made using a pipette tip. The wound healing was …

Using Scratch Wound Assay To Study The Effect Of Soil Arsenic On Human Dermal Fibroblasts Cell Migration Due To Contact Exposure, Manas Warke, Laura De Marchi, Srinivas Kannan, Madeline English, Rohan Sarkar, Rupali Datta, Smitha Rao

Michigan Tech Research Data

The scratch wound assay was performed on Normal Human Primary Dermal Fibroblasts (HDFa) cells to observe the effect on cell migration due to contact exposure to arsenic-contaminated Immokalee soil. The cell migration was observed through a microscope for 72 h. HDFa cells were seeded in 48-well plate. On day 3, treatment media was added (n=8). The cells were treated with four concentrations of soil As (45, 225, 450, and 900 mg/kg) and two controls - Negative control (NC; Pure media) and control (C; 0 mg/kg soil As) for 72 h. A scratch was made using a pipette tip. The wound …

A Comparative Metabolomics Approach For Egyptian Mango Fruits Classification Based On Uv And Uplc/Ms And In Relation To Its Antioxidant Effect, Mohamed Farag, Amr Abdelwareth, Ahmed Zayed, Tarek Eissa

Faculty Journal Articles

No abstract provided.

Ud Researcher Leads Study Using Nanotechnology To Restore Damaged Stem Cells, Dave Larsen

College of Arts and Sciences Newsroom

A University of Dayton biomedical engineer is the lead author of a study detailing a new strategy to restore damaged umbilical cord stem cells.

In-Silico Analysis Of Interacting Pathways Through Kim-1 Protein Interaction In Diabetic Nephropathy, Fatima Abid, Z Rubab, Syeda Sadia Fatima, A Qureshi, Arfa Azhar, A Jafri

Department of Biological & Biomedical Sciences

Background: Human Kidney Injury Molecule-1, also known as HAVCR-1 (Hepatitis A virus cellular receptor 1), belongs to the cell-surface protein of immunoglobulin superfamily involved in the phagocytosis by acting as scavenger receptor epithelial cells. The study focused on pinpointing the mechanisms and genes that interact with KIM-1.
Methods: This in-silico study was done from March 2019 to December 2019. The Enrichment and protein-protein interaction (PPI) network carefully choose proteins. In addition, the diagramed gene data sets were accomplished using FunRich version 3.1.3. It was done to unveil the proteins that may affect the regulation of HAVCR1 or may be regulated …

Unravelling Inventorship, Toshiko Takenaka

Chicago-Kent Journal of Intellectual Property

Inventorship, who made an invention, is one of the most important concepts under the U.S. patent system. Incorrect inventorship determinations result in patent invalidity not only because U.S. Constitution requires granting patents to true inventors, but also first-inventor- to-file novelty inherited many aspects of first-to-invent novelty which depended on inventorship whether to include prior inventions as prior art. Correcting inventorship may result in sharing patent exclusivity with competitors, which forfeits profits necessary to recover expensive development costs. However, the standard to determine inventorship has been called muddy by judges and commentators because neither the Patent Act nor case law provide …

Ethanol-Induced Impacts On Nervous System Development And Dopamine Transporter Characterization In Schmidtea Mediterranea Planaria, Brennen Covely

Theses and Dissertations

Fetal alcohol spectrum disorders (FASD) are diagnosed in 2-5% of newborns, but the biology underlying FASD is poorly understood and challenging to study with existing model organisms. A new model for FASD research is Schmidtea mediterranea (Smed) planaria. Smed have a remarkable ability to regenerate their central nervous system (CNS), and possess a well-studied, simple genome. Previous studies have shown that ethanol exposure delays this regeneration, yet this relationship is not fully understood. Here, we show that alcohol exposure affects Smed in a dose-dependent manner, eliciting characteristic withdrawal-like behaviors and impacting cognitive function. Interestingly, prior exposure does not alter subsequent …