Digital Commons Network^™

Implementation Of Preemptive Dna Sequence-Based Pharmacogenomics Testing Across A Large Academic Medical Center: The Mayo-Baylor Right 10k Study, Liewei Wang, Steven E Scherer, Suzette J Bielinski, Donna M Muzny, Leila A Jones, John Logan Black, Ann M Moyer, Jyothsna Giri, Richard R Sharp, Eric T Matey, Jessica A Wright, Lance J Oyen, Wayne T Nicholson, Mathieu Wiepert, Terri Sullard, Timothy B Curry, Carolyn R Rohrer Vitek, Tammy M Mcallister, Jennifer L St Sauver, Pedro J Caraballo, Konstantinos N Lazaridis, Eric Venner, Xiang Qin, Jianhong Hu, Christie L Kovar, Viktoriya Korchina, Kimberly Walker, Harshavardhan Doddapaneni, Tsung-Jung Wu, Ritika Raj, Shawn Denson, Wen Liu, Gauthami Chandanavelli, Lan Zhang, Qiaoyan Wang, Divya Kalra, Mary Beth Karow, Kimberley J Harris, Hugues Sicotte, Sandra E Peterson, Amy E Barthel, Brenda E Moore, Jennifer M Skierka, Michelle L Kluge, Katrina E Kotzer, Karen Kloke, Jessica M Vander Pol, Heather Marker, Joseph A Sutton, Adrijana Kekic, Ashley Ebenhoh, Dennis M Bierle, Michael J Schuh, Christopher Grilli, Sara Erickson, Audrey Umbreit, Leah Ward, Sheena Crosby, Eric A Nelson, Sharon Levey, Michelle Elliott, Steve G Peters, Naveen Pereira, Mark Frye, Fadi Shamoun, Matthew P Goetz, Iftikhar J Kullo, Robert Wermers, Jan A Anderson, Christine M Formea, Razan M El Melik, John D Zeuli, Joseph R Herges, Carrie A Krieger, Robert W Hoel, Jodi L Taraba, Scott R St Thomas, Imad Absah, Matthew E Bernard, Stephanie R Fink, Andrea Gossard, Pamela L Grubbs, Therese M Jacobson, Paul Takahashi, Sharon C Zehe, Susan Buckles, Michelle Bumgardner, Colette Gallagher, Kelliann Fee-Schroeder, Nichole R Nicholas, Melody L Powers, Ahmed K Ragab, Darcy M Richardson, Anthony Stai, Jaymi Wilson, Joel E Pacyna, Janet E Olson, Erica J Sutton, Annika T Beck, Caroline Horrow, Krishna R Kalari, Nicholas B Larson, Hongfang Liu, Liwei Wang, Guilherme S Lopes, Bijan J Borah, Robert R Freimuth, Ye Zhu, Debra J Jacobson, Matthew A Hathcock, Sebastian M Armasu, Michaela E Mcgree, Ruoxiang Jiang, Tyler H Koep, Jason L Ross, Matthew G Hilden, Kathleen Bosse, Bronwyn Ramey, Isabelle Searcy, Eric Boerwinkle, Richard A Gibbs, Richard M Weinshilboum

Faculty, Staff and Student Publications

PURPOSE: The Mayo-Baylor RIGHT 10K Study enabled preemptive, sequence-based pharmacogenomics (PGx)-driven drug prescribing practices in routine clinical care within a large cohort. We also generated the tools and resources necessary for clinical PGx implementation and identified challenges that need to be overcome. Furthermore, we measured the frequency of both common genetic variation for which clinical guidelines already exist and rare variation that could be detected by DNA sequencing, rather than genotyping.

METHODS: Targeted oligonucleotide-capture sequencing of 77 pharmacogenes was performed using DNA from 10,077 consented Mayo Clinic Biobank volunteers. The resulting predicted drug response-related phenotypes for 13 genes, including CYP2D6 …

From Zero To Thrive: A Model Of Cross-System And Cross-Sector Relational Health To Promote Early Childhood Development Across The Child-Serving Ecosystem, Alison L. Miller, Sara F. Stein, Rebeccah Sokol, Rachel Varisco, Phoebe Trout, Megan M. Julian, Julie Ribaudo, Joshua B. Kay, Natasha V. Pilkauskas, Nicole Gardner-Neblett, Todd I. Herrenkohl, Kara Zivin, Maria Muzik, Katherine L. Rosenblum

Articles

Early relational health between caregivers and children is foundational for child health and well-being. Children and caregivers are also embedded within multiple systems and sectors, or a “child-serving ecosystem”, that shapes child development. Although the COVID-19 pandemic has made this embeddedness abundantly clear, systems remain siloed and lack coordination. Fostering relational health amongst layers of this ecosystem may be a way to systematically support young children and families who are facing adversity. We integrate theory, examples, and empirical findings to develop a conceptual model informed by infant mental health and public health frameworks that illustrates how relational health across the …

The Effects Of Remote Learning On Slife Students From Central America During The Covid-19 Pandemic, Sarah Katherine Richter

Doctoral Dissertations and Projects

The Covid pandemic caused changes in education of which we may never know or understand all its repercussions to the public education system. One group of vulnerable students, newcomers from Guatemala and Honduras with limited or interrupted formal education (SLIFE), were negatively affected. Due to the COVID-19 pandemic, one SLIFE program sent its students home in the spring of 2020 to quarantine and did not return to in-person learning again until a year later. The purpose of this quantitative, causal comparative study is to investigate the effects of remote learning during the Covid-19 pandemic on SLIFE students’ education while attending …

Standardization Of A Protocol To Propagate Gateway Vectors, Kayla Suisse

Undergraduate Honors Capstone Projects

Cloning is an important technique in functional genomics, particularly genetics and molecular biology. Cloned genes can be used to facilitate protein expression; this is an essential step in understanding gene function. In traditional cloning, the gene of interest is amplified and tagged with a restriction enzyme sequence and ligated to the digested open vector. However, the GATEWAY™ Cloning Technology provides a speedy and more efficient route to insert the gene of interest in the desired vector, known as Destination vectors or Expression vectors, using site-specific recombination sites and recombinase enzymes.¹ Once DNA segments from the gene of interest are …

Factors Affecting Quality Of Life And Asthma Control In Older Adults With Asthma., Karen Schuckmann

Electronic Theses and Dissertations

Introduction: Approximately 7.7 percent of adults over the age of 65 have asthma in the United States (U.S), further, the population of older adults in the U.S. will increase to 20% by 2030. Health Related Quality of Life is low for this age group and asthma is often misdiagnosed and undertreated creating a need for research to improve factors that influence Asthma Quality of Life. Aims: This study explored two aims. The first aim evaluates the relationship between medication numeracy and asthma self-management knowledge with asthma self-efficacy. The second aim evaluates medication numeracy, asthma self-management knowledge, asthma self-efficacy, and asthma …

Aggregatibacter Actinomycetemcomitans Exploits And Modulates The Immune Response By Human Neutrophils For Survival In The Anaerobic Environment., Hazel Ozuna

Electronic Theses and Dissertations

Aggregatibacter actinomycetemcomitans (Aa) is a Gram-negative facultative anaerobe and an opportunistic oral pathogen, strongly associated with localized periodontitis and other inflammatory diseases. Periodontitis is a chronic inflammation of the periodontium resulting from the inflammatory response of the host towards the dysbiotic microbial community present at the gingival crevice. The host immune response creates a hostile environment for microorganisms; therefore, it is important for Aa to be able to regulate the necessary genes to survive and thrive in such an environment. Aaexpresses several virulence factors such as a cytolethal distending toxin (Cdt), lipopolysaccharide (LPS) and leukotoxin A (LtxA), …

Etracellular Vesicles From Lactobacillus Rhamnosus Gg Protect Against Alcohol-Induced Liver Injury Through Suppression Of Intestinal Mir 194 And Subsequent Activation Of Fxr In Mice., Mengwei Jiang

Electronic Theses and Dissertations

Numerous studies have reported the efficacy of probiotics for alcohol-associated liver disease (ALD). These studies indicate a strong interest among the scientific and medical communities in identifying alternative or adjunctive approaches for ALD, for which there is no effective or widely accepted therapeutic option. However, in-depth molecular knowledge on how probiotics render their effects is lacking. Patients with ALD often have increased hepatic bile acids (BAs), which can be toxic and are an important causative factor in liver injury and hepatocyte death. BAs are released into the intestines and serve not only as a “detergent” to promote fat absorption, but …

Understanding Public Emergency Preparedness Before And During The Coronavirus Outbreak: Implications For Effective Public Health And Risk Communication., Raphael Adjetey Fumey

Electronic Theses and Dissertations

The COVID-19 pandemic has resulted in a dramatic loss of human lives globally and presents an unprecedented challenge to public health, the world of work, and our food systems. The social and economic disruption caused by the COVID-19 pandemic is devastating, putting several millions of people at risk of falling into extreme poverty, with over 500 million people being undernourished. It is evident from the data on COVID-19 cases, deaths, and hospitalizations that the United States is the worse hit country, which can be attributed to the lack of preparedness at the individual, environmental, and government levels. This study applied …

Coping Flexibility And Academic Resilience Among Low-Ses College Students., Benjamin J. Calebs

Electronic Theses and Dissertations

College students coming from a background of poverty may experience academic impairment due to their experiences of chronic economic adversity. However, despite the stressors associated with poverty and the potential deleterious consequences of this form of adversity, many low-socioeconomic status (low-SES) college students show high academic achievement. One predictor of resilient outcomes that has been studied outside of academic contexts is coping flexibility, the ability to use a range of different coping behaviors to meet the demands of different stressful situations. Coping flexibility has been found to be positively associated with psychological adjustment in a variety of populations, yet it …

Particulate Hexavalent Chromium Inhibits Homologous Recombination Repair By Targeting Rad51 Paralogs In Human Lung Fibroblasts., Aggie R. Williams

Electronic Theses and Dissertations

Hexavalent Chromium [Cr(VI)] is a known human lung carcinogen and general health hazard. The mechanism of carcinogenesis remains poorly understood, but chromosome instability (CIN) is the major theory in its carcinogenic mechanism. Homologous recombination (HR) repair is a DNA repair pathway that prevents CIN by repairing DNA double-strand breaks. RAD51, a key mediator protein of HR repair, along with the RAD51 paralogs (RAD51B, C, D, XRCC2, and 3) are required for HR repair. During HR, RAD51 loads and forms a helical nucleoprotein filament structure to promote DNA strand exchange and stimulate pairing activity of DNA. Cr(VI) exposures have been shown …

Di•As•Po•Ra: Displaced, Not Erased., Betty Álvarez

Electronic Theses and Dissertations

This thesis is based on my artistic endeavors and research founded in my culture, family, the history of the United States government’s intervention in El Salvador, and the current political climate Latin Americans face today in the United States. I work within multiple mediums to resurrect pieces of my culture that are being forgotten or left behind due to assimilation. I accomplish this through revisiting and reinterpreting traditions. I aim to bring awareness of the Latin experience in a racially divided United States through artistic expression and my own personal experiences. My works are made to feel whimsical and playful, …

Screen Time, Sleep, And Anxiety, Michael Edward Turner

Theses, Dissertations and Culminating Projects

Prior literature has found evidence for a connection between screen time and a variety of negative health outcomes. These negative health outcomes include symptoms of both generalized and social anxiety, as well as sleep issues involving poorer sleep quality and less sleep overall. There is also evidence that screen time has dramatically increased as a result of the COVID-19 global pandemic. The present research project investigated the relationship between smartphone screen time and anxiety, both generalized and social, as well as sleep duration and sleep quality. This research project breaks down screen time into four separate domains, social media, video …

Characterization Of Gangliosides And Fatty Acids In Extracellular Vesicles From Human Milk, Aliya Amin

Food Science (MS) Theses

Extracellular vesicles (EVs) are bioactive components of human milk that may impact functionality to regulate growth, cognitive development, metabolism, and immunity in newborns. The biogenesis of EVs and the mechanism by which EVs elicit biologic effects in vivo have not been characterized entirely. Gangliosides and fatty acids are integral in the formation, release, stability, and functionalities of EVs. Presumably, EV function is related to EV composition and lipids in EVs influence the bioavailability of EV cargo and downstream functions of EVs. The objective of this research was to characterize the fatty acid and ganglioside composition of EVs in human milk. …

Effects Of Breakfast Delivery Systems And School Poverty On School And Student Outcomes In Arkansas, Matthew Mellor

College of Education Dissertations

This study investigated the effects of the breakfast in the classroom model and school poverty on attendance, discipline referrals, and fourth-grade literacy scores. The sample was composed of data from Arkansas elementary schools. Overall, the three hypotheses had no significant interaction effect. The main effect of the breakfast delivery system was not significant for discipline referrals and fourth-grade reading but was significant for school attendance. However, the practical significance was negligible. In contrast, school-wide poverty level significantly affected the percentage of scores for fourth-grade literacy in the Ready and Exceeding categories as measured by the ACT Aspire Summative Literacy Assessment …

The Health In Mental Health: Exploring Individualized Holistic Health Practices As A Process To Transform Mental Health, Craig Craig Keaton

Social Work Dissertations - Archive

Mental health problems are on the rise, especially among college students. Problematically, the two most common approaches - pharmacotherapy and talk therapy - are either grounded in a limited and suspect evidence base or is not an approach that reaches most people in need of and wanting mental health support. There is, however, a growing body of evidence supporting a health-based approach to mental health, including the application of nature, exercise, nutrition, and lifestyle. As well, with an expansive definition of health, there are even more practices, such as art, writing, and social connection to support better health and mental …

Evaluating Headstarting As A Conservation Strategy For Anegada Rock Iguanas (Cyclura Pinguis), Kelly Allison Bradley

Biology Theses - Archive

In an effort to combat the current extinction crisis, conservation biologists have developed a diverse set of tools, including conservation physiology. This scientific discipline applies physiological concepts and tools to generate conservation solutions for a broad range of species. The Anegada Rock Iguana (Cyclura pinguis) is a Critically Endangered species native to Anegada, British Virgin Islands. Feral cats depredate hatchlings within months of emergence, drastically reducing recruitment and population growth. By the 1990s, the population had declined by 80%. A headstart program was initiated in 1997 to combat this threat and increase the population. Wild collected hatchlings are reared in …

Shared Decision-Making Decision Aid Intervention To Improve Diabetes Medication Adherence, David Baba

Nursing Dissertations - Archive

A quasi-experimental study was conducted to investigate the effectiveness of a shared-decision-making (SDM) intervention using a decision aid (DA) with medication adherence and social support of African American/Black adults with type 2 diabetes. Orem's self-care theory and self-care deficit theory were the guiding frameworks to conceptually define and explain medication adherence as a self-care demand and shared decision making and social support as conditioning factors for promoting medication adherence. The medication adherence and social support levels of 31 men and women with diabetes were measured before and three months after implementing the SDM intervention. Participants' hemoglobin A1c test results were …

An Investigation Of The Texas All-State Process, Texas Honor Orchestra Process With Regard To Title I And Students In Urban School Districts From 2010-2020, And The Effect On The Social Emotional Learning Of These Students, Lacy Mccoy

Music Theses - Archive

The Texas Music Educators Association annually recognizes the outstanding student musicians and selected school ensembles in the state through the All-State and Honor Orchestra processes. Students selected for the All-State Orchestras are from high schools from around the state. Ensembles chosen to be one of the Honor Orchestras come from both junior high and high schools. Selection for these ensembles comes after months of hard work on the part of both students and teachers, as well as a rigorous selection process. The purpose of this thesis was to investigate the equity of these processes as they pertain to Title I …

Institute Of Public Health Newsletter- Volume3, Issue2, Institute Of Public Health (Iph)

Institute of Public Health Newsletter (IPHNL) and Other Publications

Volume 3, Issue 2 (May August 2022)

Regulation And Redistribution With Lives In The Balance, Daniel Hemel

University of Chicago Law Review

A central question in law and economics is whether nontax legal rules should be designed solely to maximize efficiency or whether they also should account for concerns about the distribution of income. This question takes on particular importance in the context of cost-benefit analysis. Federal agencies apply cost-benefit analysis when writing regulations that generate multibillion-dollar impacts on the U.S. economy and profound effects on millions of Americans’ lives. In the past, agencies’ cost-benefit analyses typically have ignored the income-distributive consequences of those regulations. That may soon change: on his first day in office, President Joe Biden instructed his Office of …

Racial Discrimination From A Self-Determination Lens, Lindsey Shamp

Doctoral Dissertations

Despite significant advances toward equality, racism and discrimination have been a central feature of America’s culture. This inequality has been presented from the history of slavery to the present-day violence against unarmed Black men. Oppression and inequalities have led calls to action from the African American community and activist groups including the Black Lives Matter Movement. Many White individuals are unaware of the existing benefits of their Whiteness and are simultaneously unaware of the significant psychological, emotional, physical, and social consequences for African American individuals. While there are several established theories to address the psychological and social aspects of how …

Nlr Eats: A Nonprofit To Feed Hungry Kids, Sydney Eagan

Accounting Undergraduate Honors Theses

Organization Description: Dog-Town Eats is a not-for-profit organization set up in North Little Rock, Arkansas, for the purpose of providing nutritious meals to underprivileged children on the weekends. The organization will also strive to provide meals when children are not in school over the summer months.

Specific Concept: Dog-Town Eats would like to purchase a food truck as well as supplies to offer meals. The organization will progressively expand to the areas surrounding North Little Rock and then eventually the entire state of Arkansas.

Market Description: Children who do not have the means of finding and or providing themselves …

The Typical Toddler: Diet And Approaches To Picky Eating, Carissa Adams

Occupational Therapy Capstone Presentations

To address the contested definition of picky eating, a study was conducted to determine the typical toddler diet, therefore identifying when intervention may be warranted, and further defining picky eating. Out of the 252 responses to a cross-sectional survey, 34.9% of caregivers reported their child was a picky eater, whereas 65.1% were identified as non-picky eaters. The diets and quantity of foods consumed by these groups varied significantly. Picky eaters were less likely to eat foods of potentially polarizing sensory properties or textures that required advanced oral motor skills to consume. Children who were picky eaters consumed only 32 different …

The Role Of Map Kinase Phosphatase-5 In Cardiac Adaptations To Endurance Exercise, Jaime Alfredo Perales

Open Access Theses & Dissertations

Endurance Exercise is a form of exercise that provides many benefits to the human body. Primarily, the cardiac muscle endures many cardiac adaptations in response to Endurance Exercise. But the mechanisms that are involved within these adaptations remains to be fully understood. MAPK or mitogen-activated protein kinase is a well know signaling pathway that promotes many cardiac benefits such as cell proliferation, protein synthesis and cell differentiation. Although this pathway is activated due to its upstream kinase (MKKs), the research presented focuses on the relationship and mechanisms involved in mitogen kinase phosphatase (MKPs) specifically (MKP-5). The research presented hopes to …

Contribution Of A Higher Educational Institution Towards Advancing Sustainability In The West Texas Paso Del Norte Region, Anand Raj

Open Access Theses & Dissertations

Sustainability comprises primarily three elements; environmental, social, and economic, which can be considered the keys to humanityâ??s survival and wellbeing. The pillars can alternately be given informal names: planet, people, and profit. Sustainability is achieved by effectively utilizing available resources through different sustainable development efforts. Generally, groups or entities come together to create sustainable impact in the three primary elements discussed here. City councils, businesses, and higher education institutions are just a few of the entities that engage in sustainability efforts. It is valuable to appraise the role played by a higher education institution in advancing sustainability in the context …

Genome-Wide Association Study And Genomic Prediction For Bacterial Wilt Resistance In Common Bean (Phaseolus Vulgaris) Core Collection, Bazgha Zia, Ainong Shi, Dotun Olaoye, Haizheng Xiong, Waltram Ravelombola, Paul Gepts, Howard F. Schwartz, Mark A. Brick, Kristen Otto, Barry Ogg, Senyu Chen

Horticulture Faculty Publications and Presentations

Common bean (Phaseolus vulgaris) is one of the major legume crops cultivated worldwide. Bacterial wilt (BW) of common bean (Curtobacterium flaccumfaciens pv. flaccumfaciens), being a seed-borne disease, has been a challenge in common bean producing regions. A genome-wide association study (GWAS) was conducted to identify SNP markers associated with BW resistance in the USDA common bean core collection. A total of 168 accessions were evaluated for resistance against three different isolates of BW. Our study identified a total of 14 single nucleotide polymorphism (SNP) markers associated with the resistance to BW isolates 528, 557, and 597 …

Understanding The Fate And Transport Of Per- And Polyfluoroalkyl Substances In Wastewater Systems: A New England Perspective, Sydney Kathleen Adams

Master's Theses and Capstones

This thesis explores the story of per- and polyfluoroalkyl substances (PFAS) in New England wastewater systems. PFAS compounds, known for their environmental persistence, toxic nature, and heavy integration into our daily lives, has recently become an infamous topic in environmental engineering due to the discovery of contaminated agricultural lands, water resources, and food systems. In response to these daunting discoveries, regulators have begun looking to reduce point source pollution of PFAS, including that of wastewater systems and facilities. As conduits of PFAS, wastewater systems funnel PFAS from industrial, commercial, and residential sources and emit them into the environment via aqueous …

Perceived Benefits Of Upskilling And Reskilling By Persons Who Are Among The Employed, Underemployed, And Unemployed, Rosalind Marie Evans

Electronic Theses and Dissertations

ABSTRACT The purpose of this research study is to explore the perceived influence, potential impact, and benefits that upskilling, and reskilling have on the employed, underemployed, and unemployed worker. In addition, this study seeks to explore whether the perceived benefits of upskilling and reskilling include moving above the poverty line and therefore, out of poverty in real and sustainable ways. This resulting new position and potential increase in pay could have the positive impact of moving them and their families above the poverty line. The results of this study indicate that participants perceive upskilling and reskilling to be an option …

Creation Care, Human Wellness, And Sustainability, Omoike Eric Aizenofe

Electronic Theses and Dissertations

In this thesis, I argue that creation care contributes to human wellness and to sustainable living on earth. Within contemporary contexts, humans are suffering spiritually, mentally, and emotionally. This distress impairs wellness and reduces peoples’ capacity to contribute to sustainable solutions and earth-positive impacts. Creation care entails conscious and intentional activities that are carried out for the health of the created world. Creation care contributes to human wellness, spiritually, mentally, and emotionally by offering spaces, environments, co-habitations, and interdependency among its components that support human wellness and sustainable living. Creation care helps fight spiritual, mental, and emotional distress so that …

Editorial: Fat Metabolism And Deposition In Poultry: Physiology, Genetics, Nutrition And Interdisciplinary Research, Volume I, Sami Dridi, Craig W. Maynard, Jie Wen, Elizabeth Gilbert

Poultry Science Faculty Publications and Presentations

Editorial on the Research Topic: Fat metabolism and deposition in Poultry: Physiology, Genetics, Nutrition and Interdisciplinary Research, Volume I