Digital Commons Network^™

Determinants Of Blood Acylcarnitine Concentrations In Healthy Individuals Of The European Prospective Investigation Into Cancer And Nutrition, Roland Wedekind, Joseph A. Rothwell, Vivian Viallon, Pekka Keski-Rahkonen, Julie A. Schmidt, Veronique Chajes, Vna Katzke, Theron Johnson, Maria Santucci De Magistris, Vittorio Krogh, Pilar Amiano, Carlotta Sacerdote, Daniel Redondo-Sánchez, José María Huerta, Anne Tjønneland, Pratik Pokharel, Paula Jakszyn, Rosario Tumino, Eva Ardanaz, Torkjel M. Sandanger, Anna Winkvist, Johan Hultdin, Matthias B. Schulze, Elisabete Weiderpass, Marc J. Gunter, Inge Huybrechts

Research outputs 2022 to 2026

Background & aims: Circulating levels of acylcarnitines (ACs) have been associated with the risk of various diseases such as cancer and type 2 diabetes. Diet and lifestyle factors have been shown to influence AC concentrations but a better understanding of their biological, lifestyle and metabolic determinants is needed. Methods: Circulating ACs were measured in blood by targeted (15 ACs) and untargeted metabolomics (50 ACs) in 7770 and 395 healthy participants of the European Prospective Investigation into Cancer and Nutrition (EPIC), respectively. Associations with biological and lifestyle characteristics, dietary patterns, self-reported intake of individual foods, estimated intake of carnitine and fatty …

Saliva Cortisol Diurnal Variation And Stress Responses In Term And Preterm Infants, David Q. Stoye, James P. Boardman, Clive Osmond, Gemma Sullivan, Gillian Lamb, Gill S. Black, Natalie Z. M. Homer, Nina Nelson, Elvar Theodorsson, Evalotte Mörelius, Rebecca M. Reynolds

Research outputs 2022 to 2026

Objective:

To determine if preterm birth is associated with adaptation of the hypothalamic–pituitary–adrenal (HPA) axis and whether HPA axis programming relates to the degree of prematurity (defined as extremely preterm birth at < 28 weeks or very preterm birth at 28–32 weeks gestation).

Design:

This study reports findings from a prospective birth cohort. Saliva cortisol concentrations were measured prevaccination and postvaccination, and in the morning and evening, at 4 months chronological age.

Setting:

Infants born at a single Scottish hospital.

Participants:

45 term-born, 42 very preterm and 16 extremely preterm infants.

Outcomes:

Cortisol stress response to vaccination (postvaccination minus prevaccination cortisol concentrations), diurnal slope (log-transformed morning minus log-transformed evening cortisol values) …

Evolution Of Bacillus Thuringiensis Maize Yields In South Africa, Courtney Fay Cooper

Graduate Theses and Dissertations

The economic and environmental benefits of genetically modified (GM) maize in South Africa have been well documented in previous literature. However, concerns about the longevity of these benefits, have been raised following reports of Busseola fusca developing resistance to Bacillus thuringiensis (Bt) maize in South Africa in 2006. This study uses empirical data to estimate the potential impact of insect resistance on yields and estimates the economic and food availability impacts of genetic deterioration of Bt maize. Using data from South African National Maize Cultivar trials from 1989-2018, yield gains from Bt are observed to peak for Bt maize in …

The Effectiveness Of A Career And Technical Education Program Of Study On Preparing Students For Their Chosen Career, Rigoberto Vasquez

Electronic Theses and Dissertations

Attending a 4-year university is not a goal for all high school students. Some students, as early as their first year in high school, have chosen another direction by enrolling in a career and technical education (CTE) program of study (POS). The purpose of the study was to conduct a qualitative case study documenting the outcomes of past graduates of a CTE POS located on a public comprehensive high school campus in California. The participation of former graduates to share their insight and experience from being high school age students to working adults provided the real-life ramifications of the effectiveness …

The Effect Of Increasing Intestinal Short‐Chain Fatty Acid Concentration On Gut Permeability And Liver Injury In The Context Of Liver Disease: A Systematic Review, Keith Pohl, Prebashan Moodley, Ashwin Dhanda

Peninsula Medical School

Background and AimThe gut barrier protects the liver through tight junctions, which are disrupted in liver disease either from dysbiosis, inflammation, or the effects of ingested compounds such as alcohol. Strengthening of the gut barrier may ameliorate liver injury of varying etiologies. Short chain fatty acids (SCFAs) have been shown to improve gut barrier function. This systematic review aims to synthesize all studies that have trialed SCFA supplementation as a therapy for liver disease.MethodsA systematic review assessing the impact of SCFA supplementation on liver injury and intestinal permeability was conducted. All forms of intervention that specifically increased intestinal SCFA concentration …

Application Of Microwave-Assisted Technology For Single-Pass Drying Of Rough Rice, Rebecca Mawusi Bruce

Graduate Theses and Dissertations

The 915 MHz industrial microwave is a novel technology that has the potential to achieve one-pass drying of rice within a short drying duration and reduce rice drying losses. However, processing recommendations for the commercialization of this technology for rice drying is lacking. The goal of this research is to provide processing conditions that are useful for the commercialization of the 915 MHz industrial microwave technology for rice drying. This was achieved by studying the impact of microwave specific energy and processing conditions such as harvest moisture content, milling duration, and aging on the physicochemical properties of a 915 MHz …

Applied Deep Learning: Case Studies In Computer Vision And Natural Language Processing, Md Reshad Ul Hoque

Electrical & Computer Engineering Theses & Dissertations

Deep learning has proved to be successful for many computer vision and natural language processing applications. In this dissertation, three studies have been conducted to show the efficacy of deep learning models for computer vision and natural language processing. In the first study, an efficient deep learning model was proposed for seagrass scar detection in multispectral images which produced robust, accurate scars mappings. In the second study, an arithmetic deep learning model was developed to fuse multi-spectral images collected at different times with different resolutions to generate high-resolution images for downstream tasks including change detection, object detection, and land cover …

Utilization Of Finite Element Analysis Techniques For Adolescent Idiopathic Scoliosis Surgical Planning, Michael A. Polanco

Mechanical & Aerospace Engineering Theses & Dissertations

Adolescent Idiopathic Scoliosis, a three-dimensional deformity of the thoracolumbar spine, affects approximately 1-3% of patients ages 10-18. Surgical correction and treatment of the spinal column is a costly and high-risk task that is consistently complicated by factors such as patient-specific spinal deformities, curve flexibility, and surgeon experience. The following dissertation utilizes finite element analysis to develop a cost-effective, building-block approach by which surgical procedures and kinematic evaluations may be investigated. All studies conducted are based off a volumetric, thoracolumbar finite element (FE) model developed from computer-aided design (CAD) anatomy whose components are kinematically validated with in-vitro data. Spinal ligament stiffness …

Exposure To Substance And Current Substance Among School-Going Adolescents In Timor-Leste, Abigail Esinam Adade, Kenneth Owusu Ansah, Nutifafa Eugene Yaw Dey, Francis Arthur-Holmes, Henry Ofori Duah, Pascal Agbadi

College of Nursing Faculty Research and Publications

Few studies have examined how exposure to substance influences adolescent’s use of substance in Timor-Leste. We assessed this relationship using nationally representative data from Timor-Leste to address this gap. Data was pulled from the 2015 Timor-Leste Global school-based student health survey. Data of students aged 13-17years (N = 3700) from class 7–11 across schools in Timor-Leste were analyzed for this study. Second-hand smoking exposure (AOR = 1.57 [1.31, 1.89] and parental tobacco use, AOR = 1.94 [1.54, 2.44]) was significantly related to in-school adolescent’s current use of substance after adjusting for covariates. Current substance use was also positively associated with …

Fetal Loss In Pregnant Rhesus Macaques Infected With High-Dose African-Lineage Zika Virus, Lauren E. Raasch, Kevin K. Noguchi, Nicole A. Fuhler, Et Al

2020-Current year OA Pubs

Countermeasures against Zika virus (ZIKV), including vaccines, are frequently tested in nonhuman primates (NHP). Macaque models are important for understanding how ZIKV infections impact human pregnancy due to similarities in placental development. The lack of consistent adverse pregnancy outcomes in ZIKV-affected pregnancies poses a challenge in macaque studies where group sizes are often small (4-8 animals). Studies in small animal models suggest that African-lineage Zika viruses can cause more frequent and severe fetal outcomes. No adverse outcomes were observed in macaques exposed to 1x104 PFU (low dose) of African-lineage ZIKV at gestational day (GD) 45. Here, we exposed eight pregnant …

Survivor Bond Models For Securitizing Longevity Risk, Priscilla Mansah Codjoe

Doctoral Dissertations

"Longevity risk is the risk that a reference population’s mortality rates deviate from what is projected from prior life tables. This is due to discoveries in biological sciences, improved public health measures, and nutrition, which have dramatically increased life expectancy. Longevity risk raises life insurers’ liability, increasing product costs and reserves. Securitization through longevity derivatives is a way of dealing with this risk.

To enhance the pricing of life contingent products, we present an additive type mortality model in the style of the Lee-Carter. This model incorporates policyholder covariates. By using counting processes and martingale machinery, we obtain close form …

Preventing Cholesterol-Induced Perk (Protein Kinase Rna-Like Endoplasmic Reticulum Kinase) Signaling In Smooth Muscle Cells Blocks Atherosclerotic Plaque Formation, Abhijnan Chattopadhyay, Pujun Guan, Suravi Majumder, Kaveeta Kaw, Zhen Zhou, Chen Zhang, Siddharth K Prakash, Anita Kaw, L Maximillian Buja, Callie S Kwartler, Dianna M Milewicz

Faculty, Staff and Student Publications

BACKGROUND: Vascular smooth muscle cells (SMCs) undergo complex phenotypic modulation with atherosclerotic plaque formation in hyperlipidemic mice, which is characterized by de-differentiation and heterogeneous increases in the expression of macrophage, fibroblast, osteogenic, and stem cell markers. An increase of cellular cholesterol in SMCs triggers similar phenotypic changes in vitro with exposure to free cholesterol due to cholesterol entering the endoplasmic reticulum, triggering endoplasmic reticulum stress and activating Perk (protein kinase RNA-like endoplasmic reticulum kinase) signaling.

METHODS: We generated an SMC-specific

RESULTS: SMC-specific deletion of Perk reduces atherosclerotic plaque formation in male hyperlipidemic mice by 80%. Single-cell transcriptomic data identify 2 …

Teaching Dbt Skills To Daca Recipients And Their Families: Findings From An Echo Program, Frances Morales, Oscar F. Rojas Perez, Michelle Silva, Manuel Paris, Luz M. Garcini, Melanie M. Domenech Rodriguez, Alfonso Mercado

Psychological Science Faculty Publications

Deferred Action for Childhood Arrivals (DACA) offers temporary administrative relief from deportation for undocumented immigrant adolescents and young adults who were brought as children to the United States. Accordingly, DACA has contributed to creating a different landscape of opportunities for this group. However, DACA has been and continues to be highly contested in the national political climate. Threats to DACA give rise to considerable anxiety, fear, and distress among its recipients, who face significant barriers to accessing mental health care services. Thus, a group of psychologists partnered with a leading immigrant rights advocacy organization and formed a reciprocal collaboration to …

Progressive Resistance Training For Concomitant Increases In Muscle Strength And Bone Mineral Density In Older Adults: A Systematic Review And Meta-Analysis, Steven J. O’Bryan, Catherine Giuliano, Mary N. Woessner, Sara Vogrin, Cassandra Smith, Gustavo Duque, Itamar Levinger

Research outputs 2022 to 2026

Background:

Older adults experience considerable muscle and bone loss that are closely interconnected. The efficacy of progressive resistance training programs to concurrently reverse/slow the age-related decline in muscle strength and bone mineral density (BMD) in older adults remains unclear.

Objectives:

We aimed to quantify concomitant changes in lower-body muscle strength and BMD in older adults following a progressive resistance training program and to determine how these changes are influenced by mode (resistance only vs. combined resistance and weight-bearing exercises), frequency, volume, load, and program length.

Methods:

MEDLINE/PubMed and Embase databases were searched for articles published in English before 1 June, …

Genetic Determinants Of Metabolic Biomarkers And Their Associations With Cardiometabolic Traits In Hispanic/Latino Adolescents, Daeeun Kim, Anne E. Justice, Geetha Chittoor, Estela Blanco, Marcio Almeida, Juan M. Peralta, Donna M. Lehman, Joanne E. Curran, Anthony G. Comuzzie, Ravi Duggirala, John Blangero

School of Medicine Publications

Background: Metabolic regulation plays a significant role in energy homeostasis, and adolescence is a crucial life stage for the development of cardiometabolic disease (CMD). This study aims to investigate the genetic determinants of metabolic biomarkers-adiponectin, leptin, ghrelin, and orexin-and their associations with CMD risk factors.

Methods: We characterized the genetic determinants of the biomarkers among Hispanic/Latino adolescents of the Santiago Longitudinal Study (SLS) and identified the cumulative effects of genetic variants on adiponectin and leptin using biomarker polygenic risk scores (PRS). We further investigated the direct and indirect effect of the biomarker PRS on downstream body fat percent (BF%) and …

Strategies To Maximize Academic Integrity In Online Education, Glynis Bradfield, Ray Mcallister

Faculty Publications

No abstract provided.

A Review Of Heat Stress In Chickens. Part Ii: Insights Into Protein And Energy Utilization And Feeding, Jean-Remi Teyssier, Giorgio Brugaletta, Federico Sirri, Sami Dridi, Samuel J. Rochell

Poultry Science Faculty Publications and Presentations

With the growing global demand for animal protein and rising temperatures caused by climate change, heat stress (HS) is one of the main emerging environmental challenges for the poultry industry. Commercially-reared birds are particularly sensitive to hot temperatures, so adopting production systems that mitigate the adverse effects of HS on bird performance is essential and requires a holistic approach. Feeding and nutrition can play important roles in limiting the heat load on birds; therefore, this review aims to describe the effects of HS on feed intake (FI) and nutrient digestibility and to highlight feeding strategies and nutritional solutions to potentially …

Identifying Bone Matrix Impairments In A Mouse Model Of Neurofibromatosis Type 1 (Nf1) By Clinically Translatable Techniques, Rafay Ahmed, Sasidhar Uppuganti, Shrey Derasari, Joshua Meyer, Jacquelyn S Pennings, Florent Elefteriou, Jeffry S Nyman

Faculty, Staff and Students Publications

Three-to-four percent of children with neurofibromatosis type 1 (NF1) present with unilateral tibia bowing, fracture, and recalcitrant healing. Alkaline phosphatase enzyme therapy prevented poor bone mineralization and poor mechanical properties in mouse models of NF1 skeletal dysplasia; but transition to clinical trials is hampered by the lack of a technique that i) identifies NF1 patients at risk of tibia bowing and fracture making them eligible for trial enrollment and ii) monitors treatment effects on matrix characteristics related to bone strength. Therefore, we assessed the ability of matrix-sensitive techniques to provide characteristics that differentiate between cortical bone from mice characterized by …

A Telemedicine Alternative To The 6-Minute Walk Test Using Personal Activity Trackers In Liver Transplant Candidates, Andres Duarte-Rojo, Rebecca A Brown, Pamela M Bloomer, Marie Nunez Duarte, Rachel K Grubbs, Ruben Hernaez

Faculty, Staff and Students Publications

The COVID-19 pandemic has limited liver transplant (LT) candidates access to clinics. Telehealth methods to assess frailty are needed. We developed a method to estimate the step length of LT candidates, which would permit remotely obtaining the 6-min walk test (6MWT) distance with a personal activity tracker (PAT).

METHODS: 6MWT was performed while candidates wore a PAT. On first 21 subjects (stride cohort), the step length was measured and compared with calculated one (6MWT-distance/6MWT steps). On a second cohort (PAT-6MWT; n = 116), we collected the 6MWT step count and used multivariable models to generate formulas estimating step length. We …

Salivary Biomarker Evaluation Of Chronic Pancreatitis Patients Reveals Alterations In Human Proteins, Cytokines, Prostaglandin E2 Levels, And Bacterial Diversity, Richard T Waldron, Elaina K Jones, Vincent I Anani, Jolaine M Hines, Jing Zhao, Aurelia Lugea, Marcio A Diniz, Sungjin Kim, Aida Habtezion, Kristi L Hoffman, Joseph F Petrosino, William E Fisher, Liang Li, Ryan J Lennon, Ravinder Jit Singh, Santhi Swaroop Vege, Stephen J Pandol, Mark D Topazian

Faculty, Staff and Students Publications

OBJECTIVES: Chronic pancreatitis (CP) is a chronic fibroinflammatory condition of the pancreas difficult to diagnose in early stages. Novel biomarkers useful to facilitate early diagnosis or treatment responses may be found in biofluids. Although saliva can be easily and noninvasively collected from patients, useful salivary biomarkers from CP patients have not yet been identified.

METHODS: Here, we analyzed the proteome by quantitative proteomics, cytokine/chemokine levels by Luminex analysis, prostaglandin E2 (PGE2) levels by a mass spectrometry-based assay, and bacterial species diversity by 16S ribosomal ribonucleic acid sequencing in saliva samples from confirmed CP patients and healthy controls.

RESULTS: Our results …

Editorial: Helping Scientists To Communicate Well For All Considered: Strategic Science Communication In An Age Of Environmental And Health Crises, Brianne Suldovsky, Marcia Allison, Marina Joubert, Ingrid E. Lofgren, Scott Mcwilliams

Communication Faculty Publications and Presentations

From documenting evidence-based science communication training programs to examining issues of intersectionality and inclusivity in science communication, each of the 12 articles in this special issue offers a unique perspective on science communication, public engagement, and inclusivity. The case studies of training programs provide helpful lessons learned that have broad applicability. The descriptions of how social scientific and rhetorical approaches have been used to enhance inclusive science communication offer new insights into more effective science communication practices. Our hope is that, taken together, these articles will inspire improvements in our collective ability to more effectively and equitably apply scientific research …

Randomized Placebo-Controlled Trial Of Losartan For Pediatric Nafld, Miriam B Vos, Mark L Van Natta, Niviann M Blondet, Srinivasan Dasarathy, Mark Fishbein, Paula Hertel, Ajay K Jain, Saul J Karpen, Joel E Lavine, Saeed Mohammad, Laura A Miriel, Jean P Molleston, Marialena Mouzaki, Arun Sanyal, Emily P Sharkey, Jeffrey B Schwimmer, James Tonascia, Laura A Wilson, Stavra A Xanthakos, Nash Clinical Research Network

Faculty, Staff and Students Publications

BACKGROUND AND AIMS: To date, no pharmacotherapy exists for pediatric NAFLD. Losartan, an angiotensin II receptor blocker, has been proposed as a treatment due to its antifibrotic effects.

APPROACH AND RESULTS: The Nonalcoholic Steatohepatitis Clinical Research Network conducted a multicenter, double-masked, placebo-controlled, randomized clinical trial in children with histologically confirmed NAFLD at 10 sites (September 2018 to April 2020). Inclusion criteria were age 8-17 years, histologic NAFLD activity score ≥ 3, and serum alanine aminotransferase (ALT) ≥ 50 U/l. Children received 100 mg of losartan or placebo orally once daily for 24 weeks. The primary outcome was change in ALT …

How The “Blueprint” For Agriculture In Adventist Education Can Be Relevant In The 21st Century, Katherine Koudele

Faculty Publications

No abstract provided.

A Comprehensive Review On The Use Of Herbal Dietary Supplements In The Usa, Reasons For Their Use, And Review Of Potential Hepatotoxicity, Adnan Khan, Kashyap Chauhan, Heather Ross, Natali Salinas, John Magagna, Makala Wang, Patrick Zhu, Ryan Erwin, Dina Halegoua-De Marzio

Department of Medicine Faculty Papers

Herbal and dietary supplement (HDS) use has grown exponentially in the United States. Unfortunately, the incidence of HDS-related liver injury has proportionally increased. Despite the potential for certain HDSs to cause clinically significant liver injury, they are not regulated by the Food and Drug Administration. Recent efforts have been made to regulate HDSs but are far removed from the scrutiny of prescription medications. Scant literature exists on HDSs and their risks of causing liver injury. In this comprehensive review, we examine trends of HDS use in the United States and the pathophysiologic mechanisms of drug-induced liver injury (DILI) of certain …

The Effect Of Covid-19 Pandemic-Induced Stress On Reproductive-Age Women's Menstrual Cycle Regularity, Ruaa Abduljabbar Hamid, Zahra Noah Fathi, Raida Al-Wazzan

Kesmas

Intense stress resulting from major life events can affect women's menstrual cycle. The current Coronavirus Disease 2019 (COVID-19) pandemic, impacting various sectors, is considered a major form of stress. During May-November 2020, some women in Iraq have experienced menstrual disruptions, which can happen whether they get infected with the COVID-19 or simply deals with pandemic-induced stress. Thus, this study aimed to evaluate the effect of the COVID-19 pandemic-induced stress on menstrual cycle regularity of reproductive-age women. This study used an observational cross-sectional design conducted in Iraq from May to November 2020. A sample of 500 reproductive-age women filled out an …

Strengthening Causal Inference From Randomised Controlled Trials Of Complex Interventions, Jef L. Leroy, Edward A. Frongillo Jr. Ph.D., Bezawit E. Kase, Silvia Alonso, Mario Chen, Ian Dohoo, Lieven Huybregts, Suneetha Kadiyala, Naomi M. Saville

Faculty Publications

Researchers conducting randomised controlled trials (RCTs) of complex interventions face design and analytical challenges that are not fully addressed in existing guidelines. Further guidance is needed to help ensure that these trials of complex interventions are conducted to the highest scientific standards while maximising the evidence that can be extracted from each trial. The key challenge is how to manage the multiplicity of outcomes required for the trial while minimising false positive and false negative findings. To address this challenge, we formulate three principles to conduct RCTs: (1) outcomes chosen should be driven by the intent and programme theory of …

The Significance Of Trimester-Specific Thyroid Hormones Reference Intervals In Iraqi Pregnant Women, Hani Moslem Ahmad, Moayad Aziz Alabdaly, Khairaldeen Mohammed Sulaiman

Kesmas

In Iraq, there is no obvious considerable data regarding trimester-specific reference intervals of thyroid hormones despite documented multiple physiological hormonal changes during pregnancy. Thus, this study aimed to determine trimester-specific reference intervals for serum TSH, FT3, and FT4 and assess the incidence of thyroid function test misinterpretation and misdiagnosis among pregnant women using non-pregnant reference intervals. A thyroid function test was performed for 774 enrolled pregnant women. Reference intervals of TSH, FT3, and FT4 were ascertained in each pregnancy trimester. It was then compared to the adult non-pregnant values, and the incidence of misinterpretation was later calculated. TSH and FT4 …

The Context Of Male Midwives Among Rural Communities, Marlou Savella, Glicerio A. Savella

ASEAN Journal of Community Engagement

Midwifery is a profession that provides care for women, especially during pregnancy and childbirth. Despite the increasing number of men finishing a degree in midwifery, many people assume that only women can be midwives. Thus, this study aimed to identify the context of rural communities toward the practice of midwifery by men. Further, it explored the reasons for whether they will avail of the services of male midwives. The study was conducted on 14 women and four men residing in the rural areas of Ilocos Sur, Philippines. Respondents were accessed through purposive sampling based on the inclusion criteria: A researcher-made …

Comprehensive Efforts To Accelerate Non-Communicable Disease Services In The Era Of Covid-19 In Indonesia’S Suburban Area, Akmal Taher, Soehartati A. Gondhowiardjo, Adang Bachtiar, Sabarinah Prasetyo, Irene Pixelyne, Ficky Huang, Rima Sumayyah Ahmad

ASEAN Journal of Community Engagement

The COVID-19 crisis has disrupted non-communicable disease (NCD) services in primary health care, and NCD is one of the comorbidities that increase mortality in COVID-19 patients. Efforts to accelerate NCD services were carried out with the focus on two programs: the development of integrated polyclinic NCD services and the training of community health workers (CHW) at the Tanara Community Health Center (CHC) in Banten. The development of a continuous team-based service system from the Pos Pembinaan Terpadu (Posbindu)/integrated service post to NCD services at the CHC was carried out for six months to improve the quality of human resources and …

The Development Of Video Podcast As An Innovation In Online Nutrition Education And Its Engagement Data Analysis, Lini Anisfatus Sholihah

ASEAN Journal of Community Engagement

Podcasts have become more popular in Indonesia recently. Studies have shown that video podcasts, which incorporate education and entertainment aspects, are effective as a medium to study language and medical subjects among students and clinicians. This study aims to analyze video podcasts’ utilization in nutrition education and how they can engage viewers from the general community. This paper further aims to describe the development of a video podcast, NutriPodcast, as an innovative media to educate the community regarding nutrition topics. The number of audience views was calculated by utilizing the analytical feature on social media. The methodology to develop the …