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Articles 31171 - 31200 of 31257
Full-Text Articles in Entire DC Network
Screening For Balance In Children And Adults In A Community Science Education Setting: Normative Data, Influence Of Age, Sex, And Body Mass Index, And Feasibility, Phyllis Friello, Nathan Silver, Haleh Sangi-Haghpeykar, Helen S Cohen
Screening For Balance In Children And Adults In A Community Science Education Setting: Normative Data, Influence Of Age, Sex, And Body Mass Index, And Feasibility, Phyllis Friello, Nathan Silver, Haleh Sangi-Haghpeykar, Helen S Cohen
Faculty, Staff and Students Publications
BACKGROUND: Screening standing and walking balance is useful for people suspected of having vestibular disorders, a variety of neurologic and musculoskeletal disorders, and for screening astronauts returning after exposure to microgravity. Visitors to a community science education center children and adults, aged 4 to 85, were tested on tandem walking with eyes closed and the modified Romberg test on compliant foam. They were then asked about their experience participating in research, many people for the first time.
METHODS: Subjects performed 10 steps of tandem walking with eyes closed, and three trials of the modified Romberg, or Clinical Test of Sensory …
Turning Data Into Better Mental Health: Past, Present, And Future, Nidal Moukaddam, Akane Sano, Ramiro Salas, Zakia Hammal, Ashutosh Sabharwal
Turning Data Into Better Mental Health: Past, Present, And Future, Nidal Moukaddam, Akane Sano, Ramiro Salas, Zakia Hammal, Ashutosh Sabharwal
Staff and Researcher Publications
In this mini-review, we discuss the fundamentals of using technology in mental health diagnosis and tracking. We highlight those principles using two clinical concepts: (1) cravings and relapse in the context of addictive disorders and (2) anhedonia in the context of depression. This manuscript is useful for both clinicians wanting to understand the scope of technology use in psychiatry and for computer scientists and engineers wishing to assess psychiatric frameworks useful for diagnosis and treatment. The increase in smartphone ownership and internet connectivity, as well as the accelerated development of wearable devices, have made the observation and analysis of human …
Prefrontal Glutamate Neurotransmission In Ptsd: A Novel Approach To Estimate Synaptic Strength In Vivo In Humans, Lynnette A Averill, Lihong Jiang, Prerana Purohit, Anastasia Coppoli, Christopher L Averill, Jeremy Roscoe, Benjamin Kelmendi, Henk M De Feyter, Robin A De Graaf, Ralitza Gueorguieva, Gerard Sanacora, John H Krystal, Douglas L Rothman, Graeme F Mason, Chadi G Abdallah
Prefrontal Glutamate Neurotransmission In Ptsd: A Novel Approach To Estimate Synaptic Strength In Vivo In Humans, Lynnette A Averill, Lihong Jiang, Prerana Purohit, Anastasia Coppoli, Christopher L Averill, Jeremy Roscoe, Benjamin Kelmendi, Henk M De Feyter, Robin A De Graaf, Ralitza Gueorguieva, Gerard Sanacora, John H Krystal, Douglas L Rothman, Graeme F Mason, Chadi G Abdallah
Staff and Researcher Publications
Background: Trauma and chronic stress are believed to induce and exacerbate psychopathology by disrupting glutamate synaptic strength. However, in vivo in human methods to estimate synaptic strength are limited. In this study, we established a novel putative biomarker of glutamatergic synaptic strength, termed energy-per-cycle (EPC). Then, we used EPC to investigate the role of prefrontal neurotransmission in trauma-related psychopathology.
Methods: Healthy controls (n = 18) and patients with posttraumatic stress (PTSD; n = 16) completed 13C-acetate magnetic resonance spectroscopy (MRS) scans to estimate prefrontal EPC, which is the ratio of neuronal energetic needs per glutamate neurotransmission cycle (VTCA/VCycle).
Results: Patients …
Artificial Intelligence In The Pediatric Echocardiography Laboratory: Automation, Physiology, And Outcomes, Minh B Nguyen, Olivier Villemain, Mark K Friedberg, Lasse Lovstakken, Craig G Rusin, Luc Mertens
Artificial Intelligence In The Pediatric Echocardiography Laboratory: Automation, Physiology, And Outcomes, Minh B Nguyen, Olivier Villemain, Mark K Friedberg, Lasse Lovstakken, Craig G Rusin, Luc Mertens
Faculty, Staff and Students Publications
Artificial intelligence (AI) is frequently used in non-medical fields to assist with automation and decision-making. The potential for AI in pediatric cardiology, especially in the echocardiography laboratory, is very high. There are multiple tasks AI is designed to do that could improve the quality, interpretation, and clinical application of echocardiographic data at the level of the sonographer, echocardiographer, and clinician. In this state-of-the-art review, we highlight the pertinent literature on machine learning in echocardiography and discuss its applications in the pediatric echocardiography lab with a focus on automation of the pediatric echocardiogram and the use of echo data to better …
Perspectives On Determinism In Quantum Mechanics: Born, Bohm, And The 'Quantal Newtonian' Laws, Viraht Sahni
Perspectives On Determinism In Quantum Mechanics: Born, Bohm, And The 'Quantal Newtonian' Laws, Viraht Sahni
Publications and Research
Quantum mechanics has a deterministic Schrödinger equation for the wave function. The Göttingen-Copenhagen statistical interpretation is based on the Born Rule that interprets the wave function as a ‘probability amplitude’. A precept of this interpretation is the lack of determinism in quantum mechanics. The Bohm interpretation is that the wave function is a source of a field experienced by the electrons, thereby attributing determinism to quantum theory. In this paper we present a new perspective on such determinism. The ideas are based on the equations of motion or ‘Quantal Newtonian’ Laws obeyed by each electron. These Laws, derived from the …
Extractable Entanglement From A Euclidean Hourglass, Takanori Anegawa, Norihiro Iizuka, Daniel Kabat
Extractable Entanglement From A Euclidean Hourglass, Takanori Anegawa, Norihiro Iizuka, Daniel Kabat
Publications and Research
We previously proposed that entanglement across a planar surface can be obtained from the partition function on a Euclidean hourglass geometry. Here we extend the prescription to spherical entangling surfaces in conformal field theory. We use the prescription to evaluate log terms in the entropy of a conformal field theory in two dimensions, a conformally coupled scalar in four dimensions, and a Maxwell field in four dimensions. For Maxwell we reproduce the extractable entropy obtained by Soni and Trivedi. We take this as evidence that the hourglass prescription provides a Euclidean technique for evaluating extractable entropy in quantum field theory.
Superluminal Propagation On A Moving Braneworld, Brian Greene, Daniel Kabat, Janna Levin, Arjun S. Menon
Superluminal Propagation On A Moving Braneworld, Brian Greene, Daniel Kabat, Janna Levin, Arjun S. Menon
Publications and Research
We consider a braneworld scenario in the simplest setting, M4 × S1, with a four-dimensional (4D) Minkowski metric induced on the brane, and establish the possibility of superluminal propagation. If the brane is at rest, the 4D Lorentz symmetry of the brane is exact, but if the brane is in motion, it is broken globally by the compactification. By measuring bulk fields, an observer on the brane sees a slice through a higher-dimensional field profile, which carries an imprint of the extra dimensions even when the brane is at rest. If the brane is in motion, we …
Defining Entanglement Without Tensor Factoring: A Euclidean Hourglass Prescription, Takanori Anegawa, Norihiro Iizuka, Daniel Kabat
Defining Entanglement Without Tensor Factoring: A Euclidean Hourglass Prescription, Takanori Anegawa, Norihiro Iizuka, Daniel Kabat
Publications and Research
We consider entanglement across a planar boundary in flat space. Entanglement entropy is usually thought of as the von Neumann entropy of a reduced density matrix, but it can also be thought of as half the von Neumann entropy of a product of reduced density matrices on the left and right. The latter form allows a natural regulator in which two cones are smoothed into a Euclidean hourglass geometry. Since there is no need to tensor factor the Hilbert space, the regulated entropy is manifestly gauge invariant and has a manifest state-counting interpretation. We explore this prescription for scalar fields, …
Exploratory Programming In The Arts And Humanities [Book Review], Kelly Hammond
Exploratory Programming In The Arts And Humanities [Book Review], Kelly Hammond
Publications and Research
Exploratory Programming is a testament to what open-access can mean, especially in an e-learning environment. Used in full, it is a free course (that relies on free and open software) from a gifted MIT professor whose pedagogy is clear in structure and tone. He scaffolds, promotes predictive thinking, lauds collaborative learning, and urges readers to do not just to read. Used in part, it can be equally powerful.
Quark And Gluon Entanglement In The Proton On The Light Cone At Intermediate X, Adrian Dumitru, Eric Kolbusz
Quark And Gluon Entanglement In The Proton On The Light Cone At Intermediate X, Adrian Dumitru, Eric Kolbusz
Publications and Research
In QCD with $N_c$ colors the antisymmetric valence quark color space singlet state $\sim \epsilon_{i_1\cdots i_{N_c}} |i_1,\cdots, i_{N_c}\rangle$ of the proton corresponds to the reduced density matrix $\rho_{ij}=(1/N_c) \delta_{ij}$ for a single color degree of freedom. Its degenerate spectrum of eigenvalues, $\lambda_i=1/N_c$, the purity $\tr \rho^2 = 1/N_c$, and the von Neumann entropy $S_\mathrm{vN}=\log(N_c)$ all indicate maximal entanglement of color. On the other hand, for $N_c\to\infty$ the spatial wave function of the proton factorizes into valence quark wave functions determined by a mean field [E. Witten, Nucl. Phys. B160, 57 (1979)] where there is no entanglement of spatial degrees of …
Two-Loop Four-Fermion Scattering Amplitude In Qed, R. Bonciani, A. Broggio, S. Di Vita, A. Ferroglia, M. K. Mandal, P. Mastrolia, L. Mattiazzi, A. Primo, J. Ronca, U. Schubert, W. J. Torres Bobadilla, F. Tramontano
Two-Loop Four-Fermion Scattering Amplitude In Qed, R. Bonciani, A. Broggio, S. Di Vita, A. Ferroglia, M. K. Mandal, P. Mastrolia, L. Mattiazzi, A. Primo, J. Ronca, U. Schubert, W. J. Torres Bobadilla, F. Tramontano
Publications and Research
We present the first fully analytic evaluation of the transition amplitude for the scattering of a massless into a massive pair of fermions at the two-loop level in quantum electrodynamics. Our result is an essential ingredient for the determination of the electromagnetic coupling within scattering reactions, beyond the currently known accuracy, which has a crucial impact on the evaluation of the anomalous magnetic moment of the muon. It will allow, in particular, for a precise determination of the leading hadronic contribution to the (g − 2)μ in the MUonE experiment at CERN, and therefore can be used to …
Spin(7) Orientifolds And 2d N = (0, 1) Triality, Sebastían Franco, Alessandro Mininno, Ángel M. Uranga, Xingyang Yu
Spin(7) Orientifolds And 2d N = (0, 1) Triality, Sebastían Franco, Alessandro Mininno, Ángel M. Uranga, Xingyang Yu
Publications and Research
We present a new, geometric perspective on the recently proposed triality of 2d N = (0, 1) gauge theories, based on its engineering in terms of D1-branes probing Spin(7) orientifolds. In this context, triality translates into the fact that multiple gauge theories correspond to the same underlying orientifold. We show how Spin(7) orientifolds based on a particular involution, which we call the universal involution, give rise to precisely the original version of N = (0, 1) triality. Interestingly, our work also shows that the space of possibilities is significantly richer. Indeed, general Spin(7) orientifolds extend triality to theories that can …
Bft2: A General Class Of 2d N = (0, 2) Theories, 3-Manifolds And Toric Geometry, Sebastían Franco, Xingyang Yu
Bft2: A General Class Of 2d N = (0, 2) Theories, 3-Manifolds And Toric Geometry, Sebastían Franco, Xingyang Yu
Publications and Research
We introduce and initiate the study of a general class of 2d N = (0, 2) quiver gauge theories, defined in terms of certain 2-dimensional CW complexes on oriented 3- manifolds. We refer to this class of theories as BFT2’s. They are natural generalizations of Brane Brick Models, which capture the gauge theories on D1-branes probing toric Calabi-Yau 4-folds. The dynamics and triality of the gauge theories translate into simple transformations of the underlying CW complexes. We introduce various combinatorial tools for analyzing these theories and investigate their connections to toric Calabi-Yau manifolds, which arise as their …
2d N = (0, 1) Gauge Theories And Spin(7) Orientifolds, Sebastían Franco, Alessandro Mininno, Ángel M. Uranga, Xingyang Yu
2d N = (0, 1) Gauge Theories And Spin(7) Orientifolds, Sebastían Franco, Alessandro Mininno, Ángel M. Uranga, Xingyang Yu
Publications and Research
We initiate the geometric engineering of 2d N = (0, 1) gauge theories on D1-branes probing singularities. To do so, we introduce a new class of backgrounds obtained as quotients of Calabi-Yau 4-folds by a combination of an anti-holomorphic involution leading to a Spin(7) cone and worldsheet parity. We refer to such constructions as Spin(7) orientifolds. Spin(7) orientifolds explicitly realize the perspective on 2d N = (0, 1) theories as real slices of N = (0, 2) ones. Remarkably, this projection is geometrically realized as Joyce’s construction of Spin(7) manifolds via quotients of Calabi-Yau 4-folds by antiholomorphic …
A Template Method To Measure The $T \Bar T$ Polarisation, J. A. Aguilar-Saavedra, M. C. N. Fiolhais, P. Martín-Ramiro, J. M. Moreno, António Onofre
A Template Method To Measure The $T \Bar T$ Polarisation, J. A. Aguilar-Saavedra, M. C. N. Fiolhais, P. Martín-Ramiro, J. M. Moreno, António Onofre
Publications and Research
We develop a template method for the measurement of the polarisation of $t \bar t$ pairs produced in hadron collisions. The method would allow to extract the individual fractions of $t_L \bar t_L$, $t_R \bar t_R$, $t_L \bar t_R$ and $t_R \bar t_L$ pairs with a fit to data, where L, R refer to the polarisation along any axis. These polarisation fractions have not been independently measured at present. Secondarily, the method also provides the net polarisation of t and $\bar t$, as well as their spin correlation for arbitrary axes.
Characterizing Changes In Extreme Ozone Levels Under 2050s Climate Conditions: An Extreme-Value Analysis In California, Bradley Wilson, Mariah Pope, Jeremy R. Porter, Edward Kearns, Evelyn Shu, Mark Bauer, Neil Freeman, Mike Amodeo, David Melecio-Vazquez, Ho Hsieh, Maggie Tarasovitch
Characterizing Changes In Extreme Ozone Levels Under 2050s Climate Conditions: An Extreme-Value Analysis In California, Bradley Wilson, Mariah Pope, Jeremy R. Porter, Edward Kearns, Evelyn Shu, Mark Bauer, Neil Freeman, Mike Amodeo, David Melecio-Vazquez, Ho Hsieh, Maggie Tarasovitch
Publications and Research
Ground-level ozone has a well established climate penalty associated with changing meteorological conditions under future climate change, but most existing research focuses exclusively on ozone-temperature relationships defined at specific locations. Using an extreme-value theory approach, we model relationships between temperature, humidity, and vapor pressure deficit and maximum daily 8 h ozone concentrations (MDA8) across California using a combination of ozone station network data and Community Multiscale Air Quality Modeling System (CMAQ) model output from 2008 to 2017. We use a spatial regression with time varying bias coefficients to fuse station observations and modeled output into a spatially explicit gridded ozone …
Strain‑Induced Quantum Hall Phenomena Of Excitons In Graphene, Oleg L. Berman, Roman Ya. Kezerashvili, Yurii E. Lozovik, Klaus G. Ziegler
Strain‑Induced Quantum Hall Phenomena Of Excitons In Graphene, Oleg L. Berman, Roman Ya. Kezerashvili, Yurii E. Lozovik, Klaus G. Ziegler
Publications and Research
We study direct and indirect pseudomagnetoexcitons, formed by an electron and a hole in the layers of gapped graphene under strain‑induced gauge pseudomagnetic field. Since the train‑induced pseudomagnetic field acts on electrons and holes the same way, it occurs that the properties of single pseudomagnetoexcitons, their collective effects and phase diagram are cardinally different from those of magnetoexcitons in a real magnetic field. We have derived wave functions and energy spectrum of direct in a monolayer and indirect pseudomagnetoexcitons in a double layer of gapped graphene. The quantum Hall effect for direct and indirect excitons was predicted in the monolayers …
Data From: Inventory Of Glaciers And Perennial Snowfields Of The Conterminous Usa, Andrew G. Fountain, Bryce Glenn, Christopher Mcneil
Data From: Inventory Of Glaciers And Perennial Snowfields Of The Conterminous Usa, Andrew G. Fountain, Bryce Glenn, Christopher Mcneil
Geology Faculty Datasets
This is an updated inventory of glaciers and perennial snowfields of the contiguous United States. All perennial snow and ice features greater than 0.01 km2 are included. Outlines are mostly derived from aerial imagery provided by the National Agricultural Imagery Program, US Department of Agriculture with some satellite imagery in places where aerial imagery was not suitable. Imagery dates range from 2013 to 2020. Most (70%) of the outlines were acquired from 2015 imagery.
Patterns Of Interdisciplinary Collaboration Resemble Biogeochemical Relationships In The Mcmurdo Dry Valleys, Antarctica: A Historical Social Network Analysis Of Science, 1907–2016, Stephen M. Chignell, Adrian Howkins, Poppie Gullett, Andrew G. Fountain
Patterns Of Interdisciplinary Collaboration Resemble Biogeochemical Relationships In The Mcmurdo Dry Valleys, Antarctica: A Historical Social Network Analysis Of Science, 1907–2016, Stephen M. Chignell, Adrian Howkins, Poppie Gullett, Andrew G. Fountain
Geology Faculty Publications and Presentations
Co-authorship networks can provide key insights into the production of scientific knowledge. This is particularly interesting in Antarctica, where most human activity relates to scientific research. Bibliometric studies of Antarctic science have provided a useful understanding of international and interdisciplinary collaboration, yet most research has focused on broad-scale analyses over recent time periods. Here, we take advantage of a ‘Goldilocks’ opportunity in the McMurdo Dry Valleys, an internationally important region of Antarctica and the largest ice-free region on the continent. The McMurdo Dry Valleys have attracted continuous and diverse scientific activity since 1958. It is a geographically confined region with …
A Meta-Analysis Of Studies Addressing The Impact Of Gbh On Human, Animal Health And The Environment, Kodjo Barnor
A Meta-Analysis Of Studies Addressing The Impact Of Gbh On Human, Animal Health And The Environment, Kodjo Barnor
Agribusiness and Applied Economics
The herbicide glyphosate has been tested and approved by both the FDA and the USDA, as evidenced by many published research papers, i.e., they are deemed safe for humans, animals, and the environment. However, evidence is mounting that glyphosate interferes with many metabolic processes in plants and animals, and glyphosate residues have been detected in both. The factors that influence the outcomes of previous scientific research on the potential adverse effects of GBH on human and animal health and the environment were investigated. Using DAGs and Granger causality tests, the study found that while private and public organizations were more …
Reservoir Recreation Analysis: The Effect Of Reservoir Management Agency On Recreation Participation, Mary Mena Boateng
Reservoir Recreation Analysis: The Effect Of Reservoir Management Agency On Recreation Participation, Mary Mena Boateng
Agribusiness and Applied Economics
Dams and reservoirs are created to provide flood control, water storage for irrigation and water supply, recreation, and hydroelectric generation. Most US reservoirs provide recreation in some form. However, there are concerns that certain reservoir/dam managers do a better job in providing recreation than others. Some agencies that manage land riparian to US reservoirs, such as the National Park Service (NPS), are dedicated to recreation development. Other agencies such as the Army Corps of Engineers (USACE) might not be interested in catering to the additional demands of recreators as a stakeholder group. Management actions such as boat access, waterfront land, …
Detecting The Presence Of Electronic Devices In Smart Homes Using Harmonic Radar, Beatrice Perez, Gregory Mazzaro, Timothy J. Pierson, David Kotz
Detecting The Presence Of Electronic Devices In Smart Homes Using Harmonic Radar, Beatrice Perez, Gregory Mazzaro, Timothy J. Pierson, David Kotz
Dartmouth Scholarship
Data about users is collected constantly by phones, cameras, Internet websites, and others. The advent of so-called ‘Smart Things' now enable ever-more sensitive data to be collected inside that most private of spaces: the home. The first step in helping users regain control of their information (inside their home) is to alert them to the presence of potentially unwanted electronics. In this paper, we present a system that could help homeowners (or home dwellers) find electronic devices in their living space. Specifically, we demonstrate the use of harmonic radars (sometimes called nonlinear junction detectors), which have also been used in …
Rangeland Forage Growth Prediction, Logistics, Energy, And Economics Analysis And Tool Development Using Open-Source Software, Subhashree Navaneetha Srinivasagan
Rangeland Forage Growth Prediction, Logistics, Energy, And Economics Analysis And Tool Development Using Open-Source Software, Subhashree Navaneetha Srinivasagan
Agribusiness and Biosystems Engineering
Forage availability was crucial for livestock production across the United States. Rangelands occupied vast areas 31 % of land and were the primary source of forage for livestock. However, extreme climatic conditions such as drought affect rangeland forage production and pose a serious threat to the rangeland enterprise. This increases the need to monitor forage in vast rangelands and adapt to other measures such as cultivating or buying forage to balance demand and supply. Despite this need, resources (studies and tools) on rangeland forage monitoring and existing forage production, handling, and economics were scattered and scarce. Therefore, a comprehensive systematic …
Design And Development Of An Automatic Steering System For Agricultural Towed Implements, Nadia Delavarpour
Design And Development Of An Automatic Steering System For Agricultural Towed Implements, Nadia Delavarpour
Agribusiness and Biosystems Engineering
While an auto steered tractor can improve the overall accuracy and efficiency of an operation, for operations that involve towing an implement, a significant portion of the efficiency reduction comes from uncontrolled motions of the towed implement. Therefore, there is a crucial need to study auto steering system for towed implement as well. In this study different requirements of an auto steering system for a towed implement were developed and studied. In this study the guiding performance of two local positioning sensors (Tactile and Ultrasonic sensors) under similar conditions were studied for reading different trajectories at different traveling speed. Furthermore, …
Evaluation Of The Relationship Between Size, Feeding Behavior, And Feed Efficency In Beef Cattle, Macie Mosher
Evaluation Of The Relationship Between Size, Feeding Behavior, And Feed Efficency In Beef Cattle, Macie Mosher
Animal Sciences
Feed efficiency in cow-calf operations can be influenced by multiple environmental and physiological factors. The current study examined the association between body size (weight, frame score, and volume), linear body measurements, and feeding behavior with different measures of feed efficiency in multiparous lactating beef cows. Prediction models for dry matter intake in cows using linear body measurements were also developed. Our findings indicate that body size measurements and feeding behavior may influence measures of efficiency. The inclusion of body measurements in prediction models of intake may serve as accurate and feasible methods for estimating intake of cows on farm. The …
Subito 2022, Portland State University. School Of Music & Theater
Subito 2022, Portland State University. School Of Music & Theater
Subito: Student Voices from Portland State University School of Music & Theater
Issue 5, Spring 2022
Women in electronic music, PSU's Queer Opera returns, faculty spotlight with School of Music & Theater director Bonnie Miksch, a Portland arts community survey and more.
Reconstructability Analysis: Discrete Multivariate Modeling, Martin Zwick
Reconstructability Analysis: Discrete Multivariate Modeling, Martin Zwick
Complex Systems Faculty Publications and Presentations
An introduction to Reconstructability Analysis for the Discrete Multivariate Modeling course and for other purposes.
Carli Creek Regional Water Quality Project: Assessing Water Quality Improvement At An Urban Stormwater Constructed Wetland, Christopher L. Desiderati
Carli Creek Regional Water Quality Project: Assessing Water Quality Improvement At An Urban Stormwater Constructed Wetland, Christopher L. Desiderati
Environmental Science and Management Professional Master's Project Reports
Stormwater management is an ongoing challenge in the United States and the world at-large. As state and municipal agencies grapple with conflicting interests like encouraging land development, complying with permits to control stormwater discharges, “urban stream syndrome” effects, and charges to steward natural resources for the long-term, some agencies may turn to constructed wetlands (CWs) as aesthetically pleasing and functional natural analogs for attenuating pollution delivered by stormwater runoff to rivers and streams. Constructed wetlands retain pollutants via common physical, physicochemical, and biological principles such as settling, adsorption, or plant and algae uptake. The efficacy of constructed wetlands for pollutant …
Synthetic Assembly Dna Cloning Of Multiplex Hextuple Luciferase Reporter Plasmids, Alejandro Sarrion-Perdigones, Yezabel Gonzalez, Koen J T Venken
Synthetic Assembly Dna Cloning Of Multiplex Hextuple Luciferase Reporter Plasmids, Alejandro Sarrion-Perdigones, Yezabel Gonzalez, Koen J T Venken
Faculty, Staff and Students Publications
Multiplex hextuple luciferase assaying allows monitoring the activity of five experimental pathways against one control at the same time. To perform multiplex hextuple luciferase assaying, six orthogonal luciferase reporter units are needed of which five are pathway-specific and one acts as a control for normalization. To ensure stoichiometric delivery of all six luciferase reporters in every transfected cell, synthetic assembly DNA cloning is used to stitch together all six luciferase reporter units into a single vector. Here, we provide a detailed three-step synthetic assembly DNA protocol to generate multiplex hextuple luciferase reporter plasmids for any five cellular signaling pathways of …
Current State Of The Art In Hypoplastic Left Heart Syndrome, Aditya K Birla, Sunita Brimmer, Walker D Short, Oluyinka O Olutoye, Jason A Shar, Suriya Lalwani, Philippe Sucosky, Anitha Parthiban, Sundeep G Keswani, Christopher A Caldarone, Ravi K Birla
Current State Of The Art In Hypoplastic Left Heart Syndrome, Aditya K Birla, Sunita Brimmer, Walker D Short, Oluyinka O Olutoye, Jason A Shar, Suriya Lalwani, Philippe Sucosky, Anitha Parthiban, Sundeep G Keswani, Christopher A Caldarone, Ravi K Birla
Faculty, Staff and Students Publications
Hypoplastic left heart syndrome (HLHS) is a complex congenital heart condition in which a neonate is born with an underdeveloped left ventricle and associated structures. Without palliative interventions, HLHS is fatal. Treatment typically includes medical management at the time of birth to maintain patency of the ductus arteriosus, followed by three palliative procedures: most commonly the Norwood procedure, bidirectional cavopulmonary shunt, and Fontan procedures. With recent advances in surgical management of HLHS patients, high survival rates are now obtained at tertiary treatment centers, though adverse neurodevelopmental outcomes remain a clinical challenge. While surgical management remains the standard of care for …