Digital Commons Network^™

Catalytic Enantioselective Birch–Heck Sequence For The Synthesis Of Phenanthridinone Derivatives With An All-Carbon Quaternary Stereocenter, Mary Elizabeth Sexton, William Paul Malachowski, Glenn P. A. Yap, Diana Rachii, Greg Feldman, Andrew T. Krasley, Zhilin Chen, My Anh Tran, Kalyn Wiley, Alexandra Matei, Samantha Petersen, Sabrina Tran Tien

Chemistry Faculty Research and Scholarship

Novel phenanthridinone analogues with an all-carbon quaternary stereocenter have been enantioselectively synthesized using the Birch–Heck sequence. Flat phenanthridinone structures have extensive bioactivity but consequently also suffer from poor therapeutic selectivity. The addition of a quaternary center to the phenanthridinone skeleton has the potential to generate more complex analogues with improved selectivity. Unfortunately, no general synthetic pathway to such derivatives exists. Herein we report a four-step process that transforms inexpensive benzoic acid into 22 different quaternary carbon-containing phenanthridinone analogues with a variety of substituents on all three rings: alkyl groups at the quaternary center; methyl, methoxymethyl, or para-methoxybenzyl on the …

At-Home Blood Collection And Stabilization In High Temperature Climates Using, Lauren G Brown, Amanda J Haack, Dakota S Kennedy, Karen N Adams, Jennifer E Stolarczuk, Meg G Takezawa, Erwin Berthier, Sanitta Thongpang, Fang Yun Lim, Damien Chaussabel, Mathieu Garand, Ashleigh B Theberge

Faculty Research 2022

Expanding whole blood sample collection for transcriptome analysis beyond traditional phlebotomy clinics will open new frontiers for remote immune research and telemedicine. Determining the stability of RNA in blood samples exposed to high ambient temperatures (>30°C) is necessary for deploying home-sampling in settings with elevated temperatures (e.g., studying physiological response to natural disasters that occur in warm locations or in the summer). Recently, we have developed

Development Of New Photochemical Tools For Applications In Cancer Research And Enzymatic Signaling, Nicholas Paul Toupin

Wayne State University Dissertations

Photochemical tools empower researchers to act with precision and control over the cellular environment through production of reactive oxygen species (ROS), targeted drug delivery, and modulation of enzymatic activity. These molecular tools use light as a readily available, biorthogonal reagent for initiating advantageous light-activated reactions. Using light in this way allows researchers to affect the cellular environment in specific ways with high spatio-temporal control. This dissertation focuses on expanding the applications of, mostly Ru(II)-based, photoactivated chemicals. Complexes of this type are able to act as photosensitizers in photodynamic therapy (PDT), as photocages (PCT), or as dual-action compounds capable of both …

The Imaginal Lexicon Of Aging Studies, William Ebenstein

Publications and Research

Aging Studies focuses on the cultural, humanistic and experiential aspects of aging insofar as these are already embedded in literature, art, language and everyday life. The lexicon of this multi-disciplinary field has evolved in response to shifts in cultural attitudes, social roles, and conceptual frameworks. At the same time, many age-related words have links to poetic imagery and ancient myths, and to evocative etymologies that conjure an historical context. The article looks at the ongoing and surprisingly difficult effort to find the right word to call an older person. It reviews the language and literary genres associated with longevity. And, …

The Law And Science Of Eyewitness Evidence, Thomas D. Albright, Brandon L. Garrett

Faculty Scholarship

Eyewitness evidence crucially depends on visual perception and memory, which are quite fallible. The potential inaccuracy of eyewitness memory has been long demonstrated in cases of wrongful conviction. Today, decades of scientific research on visual perception and memory have identified key causes of error and methods for improving eyewitness performance. Eyewitness evidence, where identification procedures themselves involve experiments, lends itself to scientific research as do few other areas in law. As a result, eyewitness evidence has become a testing ground for the use of science to inform the law. This Article examines how legal actors—state and federal courts, state lawmakers, …

Structural Studies Of The Nucleolar Stages Of Ribosome Biogenesis In Yeast, Linamarie Miller

Student Theses and Dissertations

The ribosome is the RNA-protein machine responsible for the essential task of translating mRNA into proteins. Ribosomes are heterodimers made up of a small subunit (SSU, 40S) and a large subunit (LSU, 60S). At the interface of these subunits, the mRNA is decoded by the small subunit and peptide bond formation is catalyzed by the rRNA of the large subunit. The cell therefore requires the timely and accurate assembly of functional ribosomal subunits, a complex process termed ribosome biogenesis. In addition to the ribosomal RNAs and ribosomal proteins that make up the mature subunits, eukaryotic cells require over 200 trans-acting …

Clerodendrum Serratum Extract Attenuates Production Of Inflammatory Mediators Inovalbumin-Induced Asthma In Rats, Poonam Arora, Shahid Hamid Ansari, Lalit Mohan Nainwal

Turkish Journal of Chemistry

In the present study, ethanolic extract of Clerodendrum serratum roots was investigated for its potential to reverse some features of bronchial asthma in ovalbumin-induced murine model of asthma. Clerodendrum serratum commonly called bharangi, (family Solanaceae) is a well-known anti-allergic drug in Asian folk system of medicines. In the present work, pharmacological studies are done to provide scientific evidence for therapeutic potential of plant in allergic asthma. Asthma was induced in experimental rats with allergen suspension of ovalbumin and aluminum hydroxide followed by treatment with dexamethasone (2.5 mg/kg, po) or C. serratum root extract (0.53 and 5.3 mg/kg, b. w., po). …

Fluorometric Determination Of Ornidazole By Using Bsa Coated Copper Nanoclusters As A Novel Turn Off Sensor, Mehmetcan Bi̇lkay, Hayri̇ye Eda Şatana Kara

Turkish Journal of Chemistry

A fluorescent probe based on bovine serum albumin stabilized copper nanoclusters (BSA-CuNCs) was developed for the selective and sensitive determination of ornidazole (ORN). The nanoclusters were synthesized via a one-pot hydrothermal process in basic media. The synthesized and characterized BSA-CuNCs have less than 3 nm particle size and exhibited blue emission at 405 nm when excited at 325 nm. Synthesized and characterized nanoclusters were successfully applied as a turn-off fluorescent probe for the determination of ORN in pharmaceutical dosage forms. The quenching mechanism created an was inner filter effect (IFE). The method was linear in the concentration range of 0.52-13.56 …

Synthesis, Antioxidant Activity, Molecular Docking And Adme Studies Of Novel Pyrrolebenzimidazolederivatives, Fi̇kri̇ye Zengi̇n Karadayi, Rahman Başaran, Mehmet Murat Kişla, Bi̇nay Eke, Zeynep Alagöz

Turkish Journal of Chemistry

Several 5-(alkylsulfonyl)-1-substituted-2-(1H-pyrrol-2-yl)-1H-benzo[d]imidazole derivatives were synthesized and their antioxidant activities were investigated using lipid peroxidation (LPO) and 7-ethoxyresorufin O-deethylase (EROD) assays. Docking analysis with Human NAD[P]H-Quinone oxidoreductase 1 (NQO1) was also performed to gather thorough information about these compounds that have antioxidant activities. Moreover, their molecular descriptors and ADME properties were calculated using the SwissADME online program. As a result, most of our compounds possessed better affinity and created ample interactions with NQO1. The most potent compound 5j had LP inhibition value of 3.73 nmol/mg/min. Other compounds exhibited moderate activity on LP levels comparing to standard butylated hydroxy toluene (BHT). However, …

Synthesis, Characterization, And Antimicrobial Evaluation Of Polyvinylalcohol-Osthol Composite Films, Ishrat Gowsia, Feroz Mir, Javid Banday

Turkish Journal of Chemistry

Although poly-vinyl alcohol (PVA) has certain mechanical drawbacks such as a weak barrier, it has widely been used in food packaging over the last many years. To increase the suitability of PVA (C₂H₄O)n and render it ideal for food packaging, a diversity of studies have already been carried out. In the below-mentioned script, we, for the first time, report the use of natural product osthol in making a new composite with PVA for enhancing thermal, physicochemical, and antimicrobial properties. The significant aim of the report is the insertion of osthol (C₁₅H₁₆O_{3 …}

Encapsulation Of Halocadmate Anion Via Hydrogen Bonding: Synthesis And Characterization Of Bis(Diethylenetriamine)Cobalt(Iii) Complex Containing Hexabromocadmate Anion, Vinit Prakash, Ritu Bala, Amrinder Kaur, Daron E. Janzen

Turkish Journal of Chemistry

In this work, the encapsulation of [CdBr₆]^4- by six cations, [Co(dien)₂]³⁺ has been described with the help of single crystal X-ray structural study in the complex, mer-[Co(dien)₂]₂[CdBr₆]Br₂. The complex anion, [CdBr₆]^4- has been obtained through solution method while attempting to synthesize complex dianion, [CdBr₄]^2-. This newly synthesized complex has been initially characterized by elemental analyses and spectroscopic studies (IR, NMR and UV-Visible). IR and NMR studies have been used for the isomeric identification of [Co(dien)₂]^{3+ …}

Transcranial Magnetic Stimulation For Post-Traumatic Stress Disorder, Amber N. Edinoff, Tanner L. Hegefeld, Murray Petersen, James C. Patterson, Christopher Yossi, Jacob Slizewski, Ashley Osumi, Elyse M. Cornett, Adam M. Kaye, Jessica S. Kaye, Vijayakumar Javalkar, Omar Viswanath, Ivan Urits, Alan David Kaye

School of Pharmacy Faculty Articles

Post-traumatic stress disorder (PTSD) is a psychiatric disorder that causes significant functional impairment and is related to altered stress response and reinforced learned fear behavior. PTSD has been found to impact three functional networks in the brain: default mode, executive control, and salience. The executive control network includes the dorsolateral prefrontal cortex (DLPFC) and lateral PPC. The salience network involves the anterior cingulate cortex, anterior insula, and amygdala. This latter network has been found to have increased functional connectivity in PTSD. Transcranial Magnetic Stimulation (TMS) is a technique used in treating PTSD and involves stimulating specific portions of the brain …

A Nationally Representative Survey Assessing Restorative Sleep In Us Adults, Rebecca Robbins, Stuart F. Quan, Daniel Buysse, Matthew D. Weaver, Matthew P. Walker, Christopher L. Drake, Kristen Monten, Laura K. Barger, Shantha M. W. Rajaratnam, Thomas Roth, Charles A. Czeisler

Sleep Medicine Articles

Restorative sleep is a commonly used term but a poorly defined construct. Few studies have assessed restorative sleep in nationally representative samples. We convened a panel of 7 expert physicians and researchers to evaluate and enhance available measures of restorative sleep. We then developed the revised Restorative Sleep Questionnaire (REST-Q), which comprises 9 items assessing feelings resulting from the prior sleep episode, each with 5-point Likert response scales. Finally, we assessed the prevalence of high, somewhat, and low REST-Q scores in a nationally representative sample of US adults (n= 1,055) and examined the relationship of REST-Q scores with other sleep …

Analysis Of Hematocrit Levels In Walleye Sander Vitreus From Similar Waterbodies, Alyssa Hauser

Journal of Earth and Life Science

Walleye Sander vitreus are a popular fish among anglers and hold a prominent place in Minnesotan’s identity as their state fish. Because of this, multiple management practices are used to ensure the continued health of their populations within Minnesota waters. In this study, the process of analyzing hematocrit levels was used to determine if the analysis of hematological parameters could be a useful tool in detecting differences in population health. To evaluate this, blood samples were acquired from age-0 and age-1 fish from two similar lakes and run through a hematocrit centrifuge to separate platelets from plasma to read the …

Codis: Community Detection Via Distributed Seed-Set Expansion On Graph Streams, Austin Anderson

Master's Projects

Community detection has been and remains a very important topic in several fields. From marketing and social networking to biological studies, community detec- tion plays a key role in advancing research in many different fields. Research on this topic originally looked at classifying nodes into discrete communities, but eventually moved forward to placing nodes in multiple communities. Unfortunately, community detection has always been a time-inefficient process, and recent data sets have been simply to large to realistically process using traditional methods. Because of this, recent methods have turned to parallelism, but all these methods, while offering sig- nificant decrease in …

Prefrontal Glutamate Neurotransmission In Ptsd: A Novel Approach To Estimate Synaptic Strength In Vivo In Humans, Lynnette A Averill, Lihong Jiang, Prerana Purohit, Anastasia Coppoli, Christopher L Averill, Jeremy Roscoe, Benjamin Kelmendi, Henk M De Feyter, Robin A De Graaf, Ralitza Gueorguieva, Gerard Sanacora, John H Krystal, Douglas L Rothman, Graeme F Mason, Chadi G Abdallah

Staff and Researcher Publications

Background: Trauma and chronic stress are believed to induce and exacerbate psychopathology by disrupting glutamate synaptic strength. However, in vivo in human methods to estimate synaptic strength are limited. In this study, we established a novel putative biomarker of glutamatergic synaptic strength, termed energy-per-cycle (EPC). Then, we used EPC to investigate the role of prefrontal neurotransmission in trauma-related psychopathology.

Methods: Healthy controls (n = 18) and patients with posttraumatic stress (PTSD; n = 16) completed 13C-acetate magnetic resonance spectroscopy (MRS) scans to estimate prefrontal EPC, which is the ratio of neuronal energetic needs per glutamate neurotransmission cycle (VTCA/VCycle).

Results: Patients …

Expression And Purification Of A Cleavable Recombinant Fortilin From Escherichia Coli For Structure Activity Studies, Maranda S. Cantrell, Jackson D. Wall, Xinzhu Pu, Matthew Turner, Luke Woodbury, Owen M. Mcdougal, Lisa R. Warner

Chemistry and Biochemistry Faculty Publications and Presentations

Complications related to atherosclerosis account for approximately 1 in 4 deaths in the United States and treatment has focused on lowering serum LDL-cholesterol levels with statins. However, approximately 50% of those diagnosed with atherosclerosis have blood cholesterol levels within normal parameters. Human fortilin is an anti-apoptotic protein and a factor in macrophage-mediated atherosclerosis and is hypothesized to protect inflammatory macrophages from apoptosis, leading to subsequent cardiac pathogenesis. Fortilin is unique because it provides a novel drug target for atherosclerosis that goes beyond lowering cholesterol and utilization of a solution nuclear magnetic resonance (NMR) spectroscopy, structure-based drug discovery approach requires milligram …

A Novel Phase And Spectroscopic Imaging Technique To Evaluate Cellular Functions, Ting Chean Khoo

Legacy Theses & Dissertations (2009 - 2024)

This thesis presents the application of Raman spectroscopy, digital holographic microscope (DHM) and transport intensity equation (TIE) in imaging biological samples. Raman spectroscopy is a non-destructive technique that can provide chemical structure, concentration, temperature, and molecular interactions. On the other hand, DHM and TIE provide physical measurements such as height, width, area and volume.We applied Raman spectroscopy to study iron-bound transferrin (Tf) in intact human breast cancer cells. Iron is an essential element required for human life and is highly regulated in the body. As the exact mechanisms of iron-bound Tf in cells are not well known, we developed a …

The Photo-Transformation Of Free Methionine In The Presence Of Surrogate And Standard Isolate Dissolved Organic Matter Under Sunlit Irradiation, Benjamin J. Mohrhardt

Dissertations, Master's Theses and Master's Reports

Sulfur (S)-containing amino acids are key sources of carbon, nitrogen, and sulfur involved in protein synthesis, protein function, and providing energy for microbial growth. Dissolved free and combined methionine is one of two S-containing amino acids incorporated into proteins and has been attributed to their stability and function. The oxidation of methionine has received considerable attention given its ubiquitous presence in most biological systems and has been associated with losses in protein function and pathological disorders. In natural waters, methionine is rapidly and selectively taken up by microorganisms to achieve cellular requirements of carbon, nitrogen, and sulfur. The abiotic transformation …

Design And Identification Of Organic Molecules As Agonists Of The Gabab Receptor And As Difluoro-C-Glycosylated Derivatives Of Flavonoids Using Molecular Modeling, Reem Alkhodier

Electronic Theses and Dissertations

The use of molecular modeling in pharmaceutical research is continuously growing. This growth is a result of the increasing number of experimental structures, the development of sophisticated modeling methods, and the improvement in computer performance by using GPUs and cloud computing. Nowadays, molecular modeling is routinely used in drug discovery to investigate biological processes and molecular properties. Herein, we use molecular modeling to investigate the behaviors of compounds with unique scaffolds.

Baclofen is the only marketed GABAB receptor agonist that is used for spasticity disorders, and it has shown promise as an anti-abuse agent. However, it has disadvantages that raise …

Stable Isotope Analysis Of A Platecarpus Tympaniticus (Squamata, Mosasauridae) With Actinocamax Sternbergi (Mollsuca, Belemnoidea) Reveals Possible Endothermic Thermoregulation, Mitchell Lukens

Master's Theses or Doctor of Nursing Practice

Mosasaurs, ancient marine reptiles, dominated the late Cretaceous oceans. However, their ecological success is a contentious topic. Were they ectothermic, like their modern relatives the varanid lizards? Or endothermic like extant marine mammals? Stable isotopes can reveal temperature and physiological variances within skeletons, but do not differentiate between body temperature and ambient environmental temperature. A rare mosasaur specimen from the Smoky Hill Chalk of a partial, articulated Platecarpus tympaniticus with stomach contents of belemnites provides a possible direct temperature contrast between predator and prey. The belemnites, related to modern coleoids, are identified as Actinocamax sternbergi. These animals possessed body …

Sustained Delivery Of Carbon Monoxide (Co) Via Organic Co Prodrugs: Pharmacokinetic Studies, Minjia Wang

Electronic Theses and Dissertations

Carbon monoxide (CO) is widely known as poisonous gas. Its role as a signaling molecule (gasotransmitter) with various therapeutic effects is less known. Recent years have seen a surge of interest in CO because of its demonstrated therapeutic effect in treating conditions such as inflammation, drug-induced toxicity, ischemia-reperfusion injury, and kidney injury, among others. In developing CO-based therapeutic agents, one key issue is the availability of pharmaceutically acceptable ways of delivering CO. During the past decade, there has been a large number of experiments in cell culture, animal models, and clinical trials using CO gas incubation/inhalation and heavy metal based …

Multivariate Statistical Modeling For Radio-Genomics Study, Tiantian Zeng

Theses and Dissertations--Statistics

Radiogenomics is a new direction in cancer research that focuses on the associations among radiomics, genomics and clinical outcome. Currently, the major challenge for Radiogenomics lies in the effective integration of genomics and imaging data for promising clinical outcome prediction. Herein, we propose a multivariate joint model that can integrate imaging and genomic data for better predicting the clinical outcome. Specifically, we jointly consider two multivariate group lasso models, one regresses imaging features on genomic features, and the other regresses patient’s clinical outcome on genomic features. An L1 penalty term is introduced for each variable, and weight in the penalty …

Development Of Zafirlukast Derivatives Active Against Porphyromonas Gingivalis, Kaitlind C. Howard

Theses and Dissertations--Pharmacy

Periodontal diseases are inflammatory diseases that can lead to damage of the soft tissue and bone supporting the teeth. Gingivitis is the reversible early stage of gum disease, which consist of gums that bleed when brushing or flossing teeth. When left untreated, the preventable but irreversible late stage of periodontal disease, periodontitis, can lead teeth to loosen or even fall out. Oral bacterial species, such as the Gram-negative anaerobic pathogen Porphyromonas gingivalis trigger these inflammatory diseases through oral dysbiosis. P. gingivalis is a keystone pathogen, meaning that the damage caused by P. gingivalis is not proportional to its abundance. Treatment …

Identifying Misconceptions About Evolution Relative To Science Curriculum Exposure At The Secondary Level, Rachel Carney

Graduate Research Papers

Evolution is the foundation of the biological sciences. Addressing misconceptions related to biological evolution ensures students have a solid understanding of evolution so that they can become scientifically literate members of society. While studies have identified misconceptions about evolution, there is much less research on the degree to which specific biology curricula address these misconceptions. The goal of this study is to determine how exposure to the SEPUP biology curriculum affects the misconceptions about evolutionary theory held by 10th grade students.

This study took place at a moderately-sized school located in the Midwest. Students respond with their agreeability to a …

W425-03c-23 - Student Organizations: Honor Societies: Beta Beta Beta, Student Organizations, Beta Beta Beta

University Archives Finding Aids

No abstract provided.

A Crew-Operated Data Recording System For Length-Based Stock Assessment Of Indonesia’S Deep Demersal Fisheries, Elle Wibisono, Peter Mous, Edwison Firmana, Austin T. Humphries

Fisheries, Animal and Veterinary Sciences Faculty Publications

Deep demersal fisheries in Indonesia yielded close to 90,000 metric tons of snapper and grouper in 2019, landed by a fleet of approximately 10,000 fishing boats. Prior to the present study, information on these multi-species, dispersed, small- to medium-scale fisheries was scarce, while reliable species-specific data on catch and effort were non-existent. This data-deficiency made stock assessments and design of harvest control rules impossible. We developed a new data collection method, the Crew Operated Data Recording System (CODRS), to collect verifiable species- and length-composition data from catches across all segments of the fleet. CODRS engaged crews of 579 fishing vessels …

The Columns, 1987 Spring

The Columns

Cover Story, "Tigers take Metro!"

MSU's Marvin Alexander (44) lofts an inside shot over Louisville's Mike Abram (34) as MSU teammate Rodney Douglas prepares to rebound in the Tigers' 75-52 victory in the 1987 Metro Conference Tournament championship game in Louisville in March. Over 18,000 watched as MSU, led by tournament Most Valuable Player Alexander, defeated the arch-rival Cardinals. Alexander and teammate Vincent Askew were both named to the All-Tournament team.

Also featured, "Executive Center Opens" by Laurie Finn

The newest building at Memphis State University - appearing from the outside as a large, four-story brick building and inside as …

Chemical Investigation Of Metabolites Produced By Marine Pseudoalteromonas Spp., Margaret Rosario

Open Access Dissertations

In natural environments, bacteria are surrounded by other organisms such as eukaryotic phytoplankton and other bacterial species. Specialized metabolites are encoded within bacterial genomes and are expected to play a large role in mediating interactions with other cells. Furthermore, chemical communication in bacterial communities is a key component to controlling group behaviors including virulence. Marine Pseudoalteromonas spp. Produce a plethora of specialized metabolites, suggesting a reliance on the biosynthesis, secretion, and response to chemical cues. This dissertation explores the prevalence and function of signaling molecules and specialized metabolites produced by two species of Pseudoalteromonas.

The first study, which is …

A Survey Of The Solitary Native Bees Of The Leach Botanical Garden, And Their Floral Associations, Joshua Page

Biology Undergraduate Honors Theses

Solitary native bees are known to be diverse, but are not well studied in Oregon. In order to identify the bee genera present in the Leach Botanical Garden, and their floral usage, a survey was conducted over 2020 and 2021. Biweekly sampling was used to collect bees on flowers, in order to obtain floral usage data. Bees were identified to genus with published taxonomic keys. In total, 19 genera of bees were found. Bee abundance fit a normal distribution, and peaked in July. Ceratina and Lasioglossum were a significant proportion of the population, and were present in the garden from …