Digital Commons Network^™

Multidimensional Single-Cell Analysis Identifies A Role For Cd2-Cd58 Interactions In Clinical Antitumor T Cell Responses, Gabrielle Romain, Paolo Strati, Ali Rezvan, Mohsen Fathi, Irfan N Bandey, Jay R T Adolacion, Darren Heeke, Ivan Liadi, Mario L Marques-Piubelli, Luisa M Solis, Ankit Mahendra, Francisco Vega, Laurence Jn Cooper, Harjeet Singh, Mike Mattie, Adrian Bot, Sattva S Neelapu, Navin Varadarajan

Faculty, Staff and Student Publications

The in vivo persistence of adoptively transferred T cells is predictive of antitumor response. Identifying functional properties of infused T cells that lead to in vivo persistence and tumor eradication has remained elusive. We profiled CD19-specific chimeric antigen receptor (CAR) T cells as the infusion products used to treat large B cell lymphomas using high-throughput single-cell technologies based on time-lapse imaging microscopy in nanowell grids (TIMING), which integrates killing, cytokine secretion, and transcriptional profiling. Our results show that the directional migration of CD19-specific CAR T cells is correlated with multifunctionality. We showed that CD2 on T cells is associated with …

The Microbiome In Gastrointestinal Cancers, Michael G White, Jennifer A Wargo

Faculty, Staff and Student Publications

The human microbiome has been recognized as increasingly important to health and disease. This is especially prescient in the development of various cancers, their progression, and the microbiome's modulation of various anticancer therapeutics. Mechanisms behind these interactions have been increasingly well described through modulation of the host immune system as well as induction of genetic changes and local inactivation of cancer therapeutics. Here, we review these associations for a variety of gastrointestinal malignancies as well as contemporary strategies proposed to leverage these associations to improve cancer treatment outcomes.

Integrated Imaging And Molecular Analysis To Decipher Tumor Microenvironment In The Era Of Immunotherapy, Jia Wu, Aaron T Mayer, Ruijiang Li

Faculty, Staff and Student Publications

Radiological imaging is an integral component of cancer care, including diagnosis, staging, and treatment response monitoring. It contains rich information about tumor phenotypes that are governed not only by cancer cellintrinsic biological processes but also by the tumor microenvironment, such as the composition and function of tumor-infiltrating immune cells. By analyzing the radiological scans using a quantitative radiomics approach, robust relations between specific imaging and molecular phenotypes can be established. Indeed, a number of studies have demonstrated the feasibility of radiogenomics for predicting intrinsic molecular subtypes and gene expression signatures in breast cancer based on MRI. In parallel, promising results …

Intermediary Role Of Lung Alveolar Type 1 Cells In Epithelial Repair Upon Sendai Virus Infection, Belinda J Hernandez, Margo P Cain, Anne M Lynch, Jose R Flores, Michael J Tuvim, Burton F Dickey, Jichao Chen

Faculty, Staff and Student Publications

The lung epithelium forms the first barrier against respiratory pathogens and noxious chemicals; however, little is known about how more than 90% of this barrier, made of AT1 (alveolar type 1) cells, responds to injury. Using the Sendai virus to model natural infection in mice, we find evidence that AT1 cells have an intermediary role by persisting in areas depleted of AT2 cells, upregulating IFN responsive genes, and receding from invading airway cells. Sendai virus infection mobilizes airway cells to form alveolar SOX2+ (Sry-box 2+) clusters without differentiating into AT1 or AT2 cells. Large AT2 cell-depleted areas remain covered by …

The Insulin Receptor Regulates The Persistence Of Mechanical Nociceptive Sensitization In Flies And Mice, Tatiana V Karpinets, Xiaogang Wu, Travis Solley, Molly B El Alam, Travis T Sims, Kyoko Yoshida-Court, Erica Lynn, Mustapha Ahmed-Kaddar, Greyson Biegert, Jingyan Yue, Xingzhi Song, Huandong Sun, Joseph F Petrosino, Melissa P Mezzari, Pablo Okhuysen, Patricia J Eifel, Anuja Jhingran, Lilie L Lin, Kathleen M Schmeler, Lois Ramondetta, Nadim Ajami, Robert R Jenq, Andrew Futreal, Jianhua Zhang, Ann H Klopp, Lauren E Colbert

Faculty, Staff and Student Publications

Early phase diabetes is often accompanied by pain sensitization. In Drosophila, the insulin receptor (InR) regulates the persistence of injury-induced thermal nociceptive sensitization. Whether Drosophila InR also regulates the persistence of mechanical nociceptive sensitization remains unclear. Mice with a sensory neuron deletion of the insulin receptor (Insr) show normal nociceptive baselines; however, it is uncertain whether deletion of Insr in nociceptive sensory neurons leads to persistent nociceptive hypersensitivity. In this study, we used fly and mouse nociceptive sensitization models to address these questions. In flies, InR mutants and larvae with sensory neuron-specific expression of RNAi …

Worldviews Transformed: How God And His People Can Change Worldview, S. Joseph Kidder, Katelyn Campbell Weakley

Faculty Publications

No abstract provided.

Whole-Genome Sequencing Links Clostridium (Clostridioides) Difficile In A Single Hospital To Diverse Environmental Sources In The Community, Su-Chen Lim, Deirdre Collins, Korakrit Imwattana, Daniel R. Knight, Sicilia Perumalsamy, Natasza M.R. Hain-Saunders, Papanin Putsathit, David Speers, Thomas Riley

Research outputs 2022 to 2026

To investigate if Clostridium (Clostridioides) difficile infection (CDI), traditionally thought of as hospital-acquired, can be genomically linked to hospital or community environmental sources, and to define possible importation routes from the community to the hospital.

In 2019, C. difficile was isolated from 89/300 (29.7%) floor and 96/300 (32.0%) shoe sole samples at a tertiary hospital in Western Australia. Non-toxigenic C. difficile ribotype (RT) 010 predominated among floor (96.6%) and shoe sole (73.2%) isolates, while toxigenic RT 014/020 was most prevalent among contemporaneous clinical cases (33.0%) at the hospital. Whole-genome sequencing and high-resolution core genome single nucleotide polymorphism (cgSNP) …

Efficacy And Safety Of Acupuncture And Or Moxibustion For Managing Primary Dysmenorrhea: A Systematic Review And Meta-Analysis, Weiting Liu, Carol Chunfeng Wang, Khui Hung Lee, Xiaopeng Ma, Timothy Leen Kang

Research outputs 2022 to 2026

Acupuncture and moxibustion have been accepted as add-on options for primary dysmenorrhea (PD); however, the clinical evidence is still inadequate. We searched AMED, CENTRAL, EMBASE, PubMed, Web of Science, CBM, CNKI, VIP, Wangfang database, ANZCTR, ClinicalTrials.gov, and the WHO ICTRP, from their inception to February 2021. The pooled analysis of 13 RCTs with 675 participants for VAS showed that acupuncture and moxibustion were more effective in managing PD than the control group with the MD of −1.93 (95% CI [−2.80, −1.06] and −2.67 (95% CI [−4.96, −0.38]). With the CMSS, seven studies with 487 participants showed that these modalities were …

Bi-Allelic Letm1 Variants Perturb Mitochondrial Ion Homeostasis Leading To A Clinical Spectrum With Predominant Nervous System Involvement, Jdc Pereira Baptista

Peninsula Medical School

Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) encodes an inner mitochondrial membrane protein with an osmoregulatory function controlling mitochondrial volume and ion homeostasis. The putative association of LETM1 with a human disease was initially suggested in Wolf-Hirschhorn syndrome, a disorder that results from de novo monoallelic deletion of chromosome 4p16.3, a region encompassing LETM1. Utilizing exome sequencing and international gene-matching efforts, we have identified 18 affected individuals from 11 unrelated families harboring ultra-rare bi-allelic missense and loss-of-function LETM1 variants and clinical presentations highly suggestive of mitochondrial disease. These manifested as a spectrum of predominantly infantile-onset (14/18, 78%) and variably progressive …

Application Of A Single-Tank Sequencing Batch Reactor For Long-Term Zebrafish Care, Aranya Ponpornpisit, Malinee Jongjaroenjai, Naowarat Suthamnatpong, Surachet Burut-Archanai

The Thai Journal of Veterinary Medicine

Sequencing batch reactors are efficient wastewater treatment systems, however, they are not adapted to treat aquaria. We aimed to compare the efficacies of a modified single-tank sequencing batch reactor (SSBR) and a regular overflow filtration system. Zebrafish were maintained in an acrylic aquarium connected to an SSBR or in a tank connected to a regular overflow filtration system for 27 weeks. Water change or aeration using air stone was not performed. The water quality in both groups was within the normal range until 26 weeks, after which the nitrite and nitrate levels increased. The average temperature was, 29.46 ± 0.71°C …

Haplotype Variation Of Partial Sry Gene In Ongole Grade Bulls (Bos Indicus) Of Indonesia, Widya Pintaka Bayu Putra, Saiful Anwar, Slamet Diah Volkandari, Syahruddin Said

The Thai Journal of Veterinary Medicine

Ongole grade cattle (Bos indicus) are Indonesian beef cattle that adapt well in a tropical climate. This study was aimed to observe the genetic diversity of partial Sex-determining of the region Y chromosome (SRY) gene (625 bp) in Ongole grade bulls (Bos indicus) of Indonesia. A total twenty-three (23) DNA samples of animal studies were collected from frozen sperm (straws) produced by the National Artificial Insemination Centers (NAICs) of Lembang (13 straws) and Singosari (10 straws). Research showed that a total of four (4) insertion mutations were detected in the intronic region with the position of 1831th, 1844th, 1850th and …

Environmental Enrichment And The Behavioral Effects On Captive Beluga Whales (Delphinapterus Leucas), Tsung-Hsien Li, Evonne Lim, I-Chun Chen, Chuen-Fu Lin, Shih-Chu Chen, Ming-An Tsai

The Thai Journal of Veterinary Medicine

Environmental enrichment methods are meant to encourage animals to display their complete ethogram while under human care in zoos and aquariums. In this study, we sought to characterize the effectiveness of environmental enrichment (involving the inclusion of toys in the living environment) on captive beluga whales in the National Museum of Marine Biology and Aquarium. Our results indicate that belugas with toys spend less time floating and swimming repetitively than do belugas without toys. The presence of toys was also shown to enhance social interactions among captive belugas. Overall, our findings indicate that the provision of toys can enrich the …

Regeneration Time: Ancient Wisdom For Planetary Wellbeing, Anne Poelina, Sandra Wooltorton, Mindy Blaise, Catrina Luz Aniere, Pierre Horwitz, Peta J. White, Stephen Muecke

Research outputs 2022 to 2026

In these regenerative times prompted by the Anthropocene, Aboriginal voices are situated to draw on ancient wisdom for local learning and to share information across the globe as ecological imperative for planetary wellbeing. In this paper, postqualitative research foregrounds the sentient nature of life as ancestral power and brings the vitality of co-becoming as our places into active engagement. It enables coloniality to surface and reveals how it sits in our places and lives, in plain sight but unnoticed because of its so-called common sense. Postqualitative research relates with ancient knowledges in foregrounding Country's animacy and presence, revealing the essence …

Esculin Hydrolysis Negative And Tcda-Only Producing Strains Of Clostridium (Clostridioides) Difficile From The Environment In Western Australia, Nirajmohan Shivaperumal, Daniel R. Knight, Korakrit Imwattana, Grace O. Androga, Barbara J. Chang, Thomas V. Riley

Research outputs 2022 to 2026

Background and Aims: Clostridium (Clostridiodes) difficile clade 3 ribotype (RT) 023 strains that fail to produce black colonies on bioMérieux ChromID agar have been reported, as well as variant strains of C. difficile that produce only toxin A. We have recently isolated strains of C. difficile from the environment in Western Australia (WA) with similar characteristics. The objective of this study was to characterize these strains. It was hypothesized that a putative β-glucosidase gene was lacking in these strains of C. difficile, including RT 023, leading to white colonies. Methods and Results: A total of 17 environmental isolates of C. …

Evaluation Of A Threonine Fermentation Product As A Digestible Threonine Source In Broilers, D. T. Lee, J. T. Lee, A. J. Ashworth, M. T. Kidd, A. Mauromostakos, S. J. Rochell

Poultry Science Faculty Publications and Presentations

In the present experiment, a Thr fermentation product (FP) (75% minimum digestible Thr) was evaluated as a replacement for crystalline L-Thr (98.5% minimum digestible Thr) as a source of digestible Thr and other nutrients to support broiler performance (0–48 d) and processing characteristics. Five phase-fed diets included 1) a positive control (PC) diet containing L-Met, L-Lys•HCl, and L-Thr, 2) a reduced protein control (RPC) diet with added L-Val, L-Ile, and L-Arg, 3) a negative control (NC) identical to the RPC except that cellulose was added at the expense of L-Thr, 4) a …

Meerkat Clan-Based Feature Selection In Random Forest Algorithm For Iot Intrusion Detection, Adil Yousef Hussein, Ahmed T.

Iraqi Journal of Computers, Communications, Control and Systems Engineering

Hackers can conduct more destructive cyber-attacks thanks to the rapid spread of Internet of Things (IoT) devices, posing significant security risks for users. Through a malicious process, the attacker intended to exhaust the capital of the target IoT network. Researchers and company owners are concerned about the reliability of IoT networks, which is taken into account because it has a significant impact on the delivery of facilities provided by IoT systems and the security of user groups. The intrusion prevention system ensures that the network is protected by detecting malicious activity. In this paper, the focus is on predicting attacks …

The Molecular Basis Of Diabetic Cardiomyopath, Ahmed Salaheldin Mustafa Ali Sultan

Dissertations

Diabetes mellitus (DM) is a major and worsening global health problem. Type 2 diabetes mellitus (T2DM) accounts for more than 90% of DM and the global epidemic of obesity largely explains the dramatic increase in the incidence and prevalence of T2DM over the past 20 years. Cardiovascular complications are the major cause of morbidity and mortality in diabetic patients. The electrocardiogram (ECG) of diabetic and obese patients is frequently disturbed. The aim of this project was to characterize and clarify the molecular basis of electro-mechanical dysfunction in the hearts of type 2 diabetic and type 2 diabetic/obese rats. Experiments were …

Temporal Lobe Epilepsy With Hippocampus Sclerosis: Prevalence, Etiology, And Treatment Options With Herbal Medicines, Muhammad Wasim, Mehr Musani, Hasan Salman Siddiqi, Saara Muddasir, Bushra Amin, Fazal Manzoor Arain

Pakistan Journal of Neurological Sciences (PJNS)

Background and objectives: Epilepsy is a neurological disorder in which the patient suffers with two or more unprovoked seizures. It is one of the most common neurological disorders worldwide, affecting more than 70 million people. Currently various modes of effective treatments are available, but still there are many cases that remain drug resistant. Hence, there is a need to explore herbal medications for the treatment of drug resistant epilepsy.

Methods: Recent reviews and research articles (2000-2022) from PubMed and Google Scholar have been explored.

Review: Based on etiology, there are six categories of epilepsy: Genetic, Structural, Metabolic, Immune, Infectious, and …

Predictors Of Early Seizures After First Acute Stroke, Malik Muhammad Adil, Sumaira Nabi, Maryam Khalil, Zeeshan Munawar, Nayab Aslam, Zaid Waqar

Pakistan Journal of Neurological Sciences (PJNS)

Background and objective: There is a lack of local data regarding the frequency and predictors of early seizures after stroke. The objective of this study was to determine the frequency of early seizures after stroke and identify the predictors which lead to them after first acute stroke.

Methods: This cross-sectional observational study was conducted in the Department of Neurology, Pakistan Institute of Medical Sciences, Islamabad from October 2021 to June 2022. A total of 310 consecutive eligible patients of acute stroke were recruited. Key inclusion criteria included any patient of >20 years of age with confirmed diagnosis of stroke on …

Characterization Of The Long Non-Coding Rna Proins In Human Pancreatic Β-Cell, Shareena Saeed Lootah

Dissertations

It is well established that the genome is pervasively transcribed. Only 2% of those transcripts are translated into proteins, and the remaining 98% are non-translated, generating the non-coding RNAs (ncRNAs) species. A close observation of these transcripts directed the scientific community to their increasing potential functional roles in the cells. Indeed, the literature exponentially unveils this notion, with many ncRNAs showing substantial and variable biological functions. The field is in its infancy; however, efforts are accelerating to discover and define this ocean of new species.

Long non-coding RNAs (lncRNAs) are sub-species of ncRNAs with an arbitrary size of more than …

Synchronous Distance Language Learning And Critical Visual Literacy Practices In Greek Primary Education, Marianthi Oikonomakou, Emmanouil Sofos, Argyro Kontogianni

Journal of Research Initiatives

Our research, focusing on critical literacy practices in education, demonstrates the outcome of a teaching scenario applied in 2021 in a primary school e-class learning environment with the aid of distant learning tools. Having taken for granted that: (a) covid-19 pandemic has initiated important changes regarding our understanding of language teaching and (b) the extend we expose ourselves to modern multimodal environments, our teaching intervention attempts at displaying how the use of visual grammar can contribute to the critical understanding and production of multimodal texts by junior pupils in the language teaching framework. In the light of the above, through …

Gothic Girlhood And Resistance: Confronting Ireland’S Neoliberal Containment Culture In Tana French’S The Secret Place, Mollie Kervick

Critical Inquiries Into Irish Studies

The Secret Place (2014) exposes a persistent Western cultural impulse to contain the emotions of teenage girls when they demonstrate control over their lives. In the Irish context, the dismissal of teenage girls is resonant of a containment culture in which controlling women’s bodies and minds has been essential to upholding heteropatriarchal ideals. Resistance to the novel’s unresolved supernatural elements by readers and critics and the lack of sustained academic scholarship also point to an unsettling complacency with the neoliberal impulse to contain female emotion and lived experience in post-Celtic Tiger Ireland.

Crime On The Periphery: Tana French’S Criminal Geography, Deirdre Flynn

Critical Inquiries Into Irish Studies

This article will analyze how Tana French conceptualizes spatiality, focusing on her use of liminal spaces, edgelands and peripheries, as the settings for her crime scenes. Instead of more traditional Irish literary urban-rural binaries, French exploits the interface of both places, reflecting a contemporary post-industrial, post-Celtic Tiger Ireland. In particular, in In the Woods (2007) the untamed woodland behind the housing estate in Knocknaree becomes an interfacial zone between the rural and urban, past and present. In The Likeness (2009), Whitethorn House sits at the edge of the village geographically, politically, and historically. In French’s first two novels peripheral spaces …

Tana French: An Interview With Brian Cliff, Brian Cliff

Critical Inquiries Into Irish Studies

No abstract provided.

How Facial Features Convey Attention In Stationary Environments, Janelle Domantay, Brendan Morris

Spectra Undergraduate Research Journal

Awareness detection technologies have been gaining traction in a variety of enterprises; most often used for driver fatigue detection, recent research has shifted towards using computer vision technologies to analyze user attention in environments such as online classrooms. This paper aims to extend previous research on distraction detection by analyzing which visual features contribute most to predicting awareness and fatigue. We utilized the open-source facial analysis toolkit OpenFace in order to analyze visual data of subjects at varying levels of attentiveness. Then, using a Support-Vector Machine (SVM) we created several prediction models for user attention and identified the Histogram of …

The Utilization Of Crispr/Cas9 In Monogenic Disorders Authors, Shauna M. Mellor

Spectra Undergraduate Research Journal

This paper is a literature review of various scientific research papers, exploring the recent scientific advancement in the field of genetic engineering. The research presented is a foundational tool, building awareness on the implications of CRISPR/ Cas9 technology. CRISPR/ Cas9 was first discovered through the study of bacterial immune systems, fighting against viral infections. Manipulation of the Cas9 protein would eventually lead to target specific, gene-altering medicines for human organisms. CRISPR/ Cas 9 technology has begun to show promise as an effective treatment for certain monogenic disorders. Despite this, time is required before its efficacy as a proven genetic treatment …

Why Imre Lakatos’S Account Of Science Is Superior, Kyle J. Q. Catarata

Spectra Undergraduate Research Journal

An important question that has always been debated in philosophy of science concerns that of the best account of science. Ranging from multiple accounts, from Karl Popper, Thomas Kuhn, and Imre Lakatos, etc., an argument of how Lakatos’s Methodology of Scientific Research Programmes (MSRP) is the best account of science will be defended. In addition, the best account of scientific evidence and explanation – featuring philosophers from Lakatos, Peter Achinstein, Nancy Cartwright, and Philip Kitcher – is presented in support of Lakatos’s MSRP. Furthermore, using the scientific theory of continental drift, proposed by Alfred L. Wegener, will illustrate how Wegener’s …

Narrative Review Of Covid-19 Vaccine Hesitancy: Evidence In Late 2021, Ambree Papa Schoetker, Manoj Sharma

Spectra Undergraduate Research Journal

The coronavirus disease, COVID-19, has changed daily life dramatically since early 2020. Although COVID-19 vaccines are available in the United States, many express distrust in this primary prevention measure and doubt both the seriousness of COVID-19 and its associated morbidity and mortality. Vaccine hesitancy, also described as the reluctance or refusal of vaccines despite availability, exists on a continuum and was a known public health threat prior to the coronavirus pandemic. This narrative review examines studies related to the determinants of COVID-19 vaccine hesitancy among adults in the United States. Also explored are the factors related to COVID-19 vaccine risk …

2022 Undergraduate Research Symposium: Full Program, Honors College, Old Dominion University

Undergraduate Research Symposium

No abstract provided.

Brainless But Smart: Investigating Cognitive-Like Behaviors In The Acellular Slime Mold Physarum Polycephalum, Subash Kusum Ray

Dissertations

Evolutionary pressures to improve fitness, have enabled living systems to make adaptive decisions when faced with heterogeneous and changing environmental and physiological conditions. This dissertation investigated the mechanisms of how environmental and physiological factors affect the behaviors of non-neuronal organisms. The acellular slime mold Physarum polycephalum was used as the model organism, which is a macroscopic, unicellular organism, that self-organizes into a network of intersecting tubules. Without using neurons, P. polycephalum can solve labyrinth mazes, build efficient tubule networks, and make adaptive decisions when faced with complicated trade-offs, such as between food quality and risk, speed and accuracy, and exploration …