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A Serotonin-Mediated Aversive Mechanism Influences Individually Variable Resilience Toacquire And Relapse To Cocaine-Seeking, Ying S. Chao

MUSC Theses and Dissertations

Although cocaine and other psychostimulants are highly rewarding, they also produce aversive effects that influence drug-seeking, and that are stronger in some individuals than others for poorly understood reasons. We now find in rats that cocaine depolarizes neurons of the rostromedial tegmental nucleus (RMTg), a major GABAergic afferent to midbrain dopamine neurons, and a driver of punishment learning. This activation is mediated by post-synaptic serotonin 2C receptors, and is particularly strong in subsets of animals having heightened aversive responses to cocaine, among whom it slows the initial acquisition of cocaine self-administration and reduced cue-induced relapse for cocaine after extinction. These …

Crispr-Krispr: A Method To Identify On-Target And Random Insertion Of Donor Dnas And Their Characterization In Knock-In Mice, Masayuki Tanaka, Keiko Yokoyama, Hideki Hayashi, Sanae Isaki, Kanae Kitatani, Ting Wang, Hisako Kawata, Hideyuki Matsuzawa, Channabasavaiah B. Gurumurthy, Hiromi Miura, Masato Ohtsuka

Journal Articles: Pharmacology & Experimental Neuroscience

CRISPR tools can generate knockout and knock-in animal models easily, but the models can contain off-target genomic lesions or random insertions of donor DNAs. Simpler methods to identify off-target lesions and random insertions, using tail or earpiece DNA, are unavailable. We develop CRISPR-KRISPR (CRISPR-Knock-ins and Random Inserts Searching PRotocol), a method to identify both off-target lesions and random insertions. CRISPR-KRISPR uses as little as 3.4 μg of genomic DNA; thus, it can be easily incorporated as an additional step to genotype founder animals for further breeding.

Ecdysoneless Overexpression Drives Mammary Tumorigenesis Through Upregulation Of C-Myc And Glucose Metabolism, Bhopal C. Mohapatra, Sameer Mirza, Aditya Bele, Channabasavaiah B. Gurumurthy, Mohsin Raza, Irfana Saleem, Matthew D. Storck, Aniruddha Sarkar, Sai Sundeep Kollala, Surendra K. Shukla, Siddesh Southekal, Kay-Uwe Wagner, Fang Qiu, Subodh M. Lele, Mansour A. Alsaleem, Emad A. Rakha, Chittibabu Guda, Pankaj K. Singh, Robert D. Cardiff, Hamid Band, Vimla Band

Journal Articles: Pharmacology & Experimental Neuroscience

Ecdysoneless (ECD) protein is essential for embryogenesis, cell-cycle progression, and cellular stress mitigation with an emerging role in mRNA biogenesis. We have previously shown that ECD protein as well as its mRNA are overexpressed in breast cancer and ECD overexpression predicts shorter survival in patients with breast cancer. However, the genetic evidence for an oncogenic role of ECD has not been established. Here, we generated transgenic mice with mammary epithelium-targeted overexpression of an inducible human ECD transgene (ECDTg). Significantly, ECDTg mice develop mammary hyperplasia, preneoplastic lesions, and heterogeneous tumors with occasional lung metastasis. ECDTg tumors exhibit epithelial to mesenchymal transition …

Biofilms: Formation, Research Models, Potential Targets, And Methods For Prevention And Treatment, Yajuan Su, Jaime T. Yrastorza, Mitchell Matis, Jenna Cusick, Siwei Zhao, Guangshun Wang, Jingwei Xie

Journal Articles: Pathology and Microbiology

Due to the continuous rise in biofilm-related infections, biofilms seriously threaten human health. The formation of biofilms makes conventional antibiotics ineffective and dampens immune clearance. Therefore, it is important to understand the mechanisms of biofilm formation and develop novel strategies to treat biofilms more effectively. This review article begins with an introduction to biofilm formation in various clinical scenarios and their corresponding therapy. Established biofilm models used in research are then summarized. The potential targets which may assist in the development of new strategies for combating biofilms are further discussed. The novel technologies developed recently for the prevention and treatment …

Methamphetamine Induces The Release Of Proadhesive Extracellular Vesicles And Promotes Syncytia Formation: A Potential Role In Hiv-1 Neuropathogenesis, Subhash Chand, Catherine Demarino, Austin Gowen, Maria Cowen, Sarah Al-Sharif, Fatah Kashanchi, Sowmya V. Yelamanchili

Journal Articles: Anesthesiology

Despite the success of combinational antiretroviral therapy (cART), the high pervasiveness of human immunodeficiency virus-1 (HIV)-associated neurocognitive disorders (HAND) poses a significant challenge for society. Methamphetamine (meth) and related amphetamine compounds, which are potent psychostimulants, are among the most commonly used illicit drugs. Intriguingly, HIV-infected individuals who are meth users have a comparatively higher rate of neuropsychological impairment and exhibit a higher viral load in the brain than infected individuals who do not abuse meth. Effectively, all cell types secrete nano-sized lipid membrane vesicles, referred to as extracellular vesicles (EVs) that can function as intercellular communication to modulate the physiology …

Distinct Synaptic Vesicle Proteomic Signatures Associated With Pre- And Post-Natal Oxycodone-Exposure, Katherine E. Odegaard, Gabriel Gallegos, Sneh Koul, Victoria L. Schaal, Neetha N. Vellichirammal, Chittibabu Guda, Andrea P. Dutoit, Steven J. Lisco, Sowmya V. Yelamanchili, Gurudutt Pendyala

Journal Articles: Anesthesiology

The current opioid crisis, which has ravaged all segments of society, continues to pose a rising public health concern. Importantly, dependency on prescription opioids such as oxycodone (oxy) during and after pregnancy can significantly impact the overall brain development of the exposed offspring, especially at the synapse. A significant knowledge gap that remains is identifying distinct synaptic signatures associated with these exposed offspring. Accordingly, the overall goal of this current study was to identify distinct synaptic vesicle (SV) proteins as signatures for offspring exposed to oxy in utero (IUO) and postnatally (PNO). Using a preclinical animal model that imitates oxycodone …

Differential Methylation Patterns In Lean And Obese Non-Alcoholic Steatohepatitis-Associated Hepatocellular Carcinoma, Emma Hymel, Kurt W. Fisher, Evi A. Farazi

Journal Articles: Epidemiology

BACKGROUND: Nonalcoholic fatty liver disease affects about 24% of the world's population and may progress to nonalcoholic steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma (HCC). While more common in those that are obese, NASH-HCC can develop in lean individuals. The mechanisms by which HCC develops and the role of epigenetic changes in the context of obesity and normal weight are not well understood.

METHODS: In this study, we used previously generated mouse models of lean and obese HCC using a choline deficient/high trans-fat/fructose/cholesterol diet and a choline supplemented/high trans-fat/fructose/cholesterol diet, respectively, to evaluate methylation differences in HCC progression in lean versus …

Lecturas Contra Un Mundo Corrupto: La Buena Lectura (1879-1882), Andrea Castro

Decimonónica

En el poema “La estrella y la tempestad,” firmado por C. y V, acudimos a un diálogo entre una niña y su madre en un paisaje que—si bien invita a una lectura alegórica—la madre y la niña experimentan en sus cuerpos.¹ La tempestad despierta en ellas sentimientos de confusión, miedo, misterio y estremecimiento. Así, la fuerza de la naturaleza evoca el sentimiento de lo sublime, sentimiento que hacia el final del poema será contenido por el espacio de la casa, construido a través de ruidos y sentimientos familiares.²

El Príncipe Entre Los Leones: El Acontecimiento Darío En La Poesía Colombiana, David Ramírez

Decimonónica

Si Colombia fuera un animal, sería un león. Al menos eso pensaba Rubén Darío, para quien el león encarnaba el rasgo más característico de lo colombiano: la combinación de violencia y refinamiento, inteligencia y conservadurismo. La referencia más conocida a este respecto es el verso inicial de su soneto “Colombia,” uno de los poemas más declamados en la historia de ese país. “Colombia es una tierra de leones,” dice allí con grandilocuencia el poeta nicaragüense (Poesías completas 726). Variaciones de este verso, o incluso su repetición, son frecuentes siempre que escribe sobre autores, políticos o temas colombianos. “Cave …

Juan De La Rosa O El Mestizo Huérfano Hispanizado Como Fachada De Modernidad, Mercedes Mayna-Medrano

Decimonónica

En el siglo XIX, surge el tropo del mestizo huérfano.¹ Esta figura condensa los deseos de las élites criollas y su intento por “resolver” el llamado problema indígena, especialmente en una Bolivia derrotada por Chile después de la Guerra del Pacífico (1879-1883).² El presente artículo tiene como objetivo central analizar la canónica novela fundacional boliviana Juan de la Rosa. Memorias del último soldado de la independencia (1885) del cochabambino Nataniel Aguirre. Mi hipótesis es que, a través del uso del modo expresivo melodramático, el autor pretende borrar la herencia indígena en los mestizos cochabambinos. Pero, a la …

Emotional Distress Recovery For Mishandling Of Human Remains: A Fifty State Survey, Christopher Ogolla

Faculty Scholarship

No abstract provided.

Psychological Impacts Of Covid-19 On Older Adults, Maria Coletta Angiolillo

Renée Crown University Honors Thesis Projects - All

No abstract provided.

Delineating Erasure: Architectural Representation As A Language For Thought, Shiori Green

Renée Crown University Honors Thesis Projects - All

No abstract provided.

Rational Design And In Vitro Screening Of Monomeric Peptides With Multi-Agonism To Simultaneously Treat Diabetes And Obesity, Lindy Melegari

Renée Crown University Honors Thesis Projects - All

No abstract provided.

Evolving Standards Of Irrelevancy?, Joanmarie Davoli

Faculty Scholarship

No abstract provided.

Ubiquitous Microplastics In The Upper Gastrointestinal Tracts Of Florida Coastal Seabirds, Jonathan J. Clark, Dorothy-Ellen A. Renegar, Dave Kerstetter

Marine & Environmental Sciences Faculty Articles

Plastic pollution is increasingly recognized as a global problem. In particular, plastic pieces <5 mm in size (‘microplastics’) are of interest due to their prevalence and association with harmful, persistent organic pollutants (POPs). Very little is known about the prevalence of microplastics in coastal birds. Yet, these water-associated birds are at a high risk of ingesting microplastics that accumulate near the water’s surface. This study describes the microplastics found in the proventriculus and ventriculus of four species of coastal birds regarding quantity, size, type (fiber or fragment), and color (light, mid, or dark). A total of 643 microplastic particles were identified, with 43 of the 44 study specimens containing microplastics (97.7% frequency). The ‘fiber’ type and the ‘mid’ color were the most common microplastics. There were no significant differences between species for particle sizes, but Brown Pelicans contained significantly more particles than the other three species. These results highlight the prevalence of plastic pollution in medium-sized seabirds, but more work is needed to determine microplastic patterns between taxa and foraging environments.

Annual Report Of The Town Officers Of The Town Of Jefferson, Nh For The Year Ending December 31, 2021., Jefferson Town Representatives

Jefferson, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

Think Again:An Original Reality Competition Series, Adam Sperling

Renée Crown University Honors Thesis Projects - All

No abstract provided.

Coral Reef Restoration Methods In The Caribbean And Florida Keys, Melissa Wagner

OUR Journal: ODU Undergraduate Research Journal

There has been a massive decline in coral population worldwide, but the Caribbean Sea has been particularly impacted. The Caribbean was once the home to millions of branching coral, Acropora, but has seen severe degradation in the past few decades due to climate change, pollution, coral disease, bleaching events, overfishing, coastal development and other disturbances. Coral reef restoration has since expanded rapidly with thousands of coral nurseries in the Caribbean alone and has become an important process in maintaining coral reefs in our oceans today. Corals help protect our shorelines, maintain biodiversity in our oceans, provide jobs for fisheries …

Nonparty Jurisdiction, Aaron D. Simowitz, Linda J. Silberman

Faculty Articles

The Supreme Court's recent decisions on personal jurisdiction, including its 2021 decision in Ford Motor Co. v. Montana Eighth Judicial District Court, have all focused on the adjudication of plenary claims. In seven years, the Court has decided six major cases on personal jurisdiction in that context. However, these precedents also appear to guide lower courts in areas outside the traditional focus of personal jurisdiction doctrine but where personal jurisdiction is nonetheless necessary. For example, a court must have personal jurisdiction over a nonparty witness in order to compel the witness to testify or to produce documents. A court must …

Strict Scrutiny & The Black Body, Devon W. Carbado

Faculty Articles

When people in law think about strict scrutiny, often they are also thinking about equal protection law’s treatment of race. For more than four decades, scholars have vigorously challenged that legal regime. Yet none of that contestation has interrogated the social manifestation of strict scrutiny. This Article does that work. Its central claim is that Black people live under a social regime of strict scrutiny that treats the mere sight of Blackness as a suspect classification. This social regime trades on some of the same racial logics that underwrite the legal regime. Like its legal counterpart, the social version of …

Budget For The Fiscal Year Ending June 30, 2022, University Of Arkansas, Fayetteville

Budget for the Fiscal Year

No abstract provided.

Voluntary Control Of Breathing According To The Breathing Pattern During Listening To Music And Non-Contact Measurement Of Heart Rate And Respiration, Dibyajyoti Biswal

Theses and Dissertations--Biomedical Engineering

We investigated if listening to songs changes breathing pattern which changes autonomic responses such as heart rate (HR) and heart rate variability (HRV) or change in breathing pattern is a byproduct of listening to songs or change in breathing pattern as well as listening to songs causes changes in autonomic responses. Seven subjects (4 males and 3 females) participated in a pilot study where they listened to two types of songs and used a custom developed biofeedback program to control their breathing pattern to match the one recorded during listening to the songs.

Coherencies between EEG, breathing pattern and RR …

Escaping Modesto: George Lucas, Film Auteur, And The Alteration Of Movie History, Krister Persson

Undergraduate Honors Theses

This video essay analyzes the early filmography of George Lucas as a critique of auteur theory. Dissecting THX 1138 (1971), American Graffiti (1973), and Star Wars (1977) based on their autobiographic qualities, this short documentary investigates the advantages and disadvantages of viewing a complicated cinematic work through the lens of an individual author. Analysis focuses on Lucasfilm founder George Lucas and his reputation for controversially altering his works through special edition re-releases, calling into question the sanctity of theatrical releases and the confines of a film auteur. Does a filmmaker, particularly one whose films are drawn from personal experience, have …

Using Land And Water Conservation Fund Money To Protect Western Migration Corridors, Travis Brammer

Wyoming Law Review

No abstract provided.

Grizzlies, Wolves, And Law In The Greater Yellowstone Ecosystem: Wildlife Management Amidst Jusidictional Complexity And Tension, Robert Keiter

Wyoming Law Review

No abstract provided.

Re-Indigenizing Yellowstone, Kekek Jason Stark, Autumn L. Bernhardt, Monte Mills, Jason A. Robison

Wyoming Law Review

No abstract provided.

Annual Report: Nebraska Extension In Lancaster County, January 1, 2022 Through December 31, 2022

Lancaster County, Nebraska Extension: Reports

Lancaster County Extension information from 2022. Contents include:

1) About Extension - Lancaster County Extension mission statement, overview and impact reports;

2) Staff and Programs - staff directory and Extension Educator annual program summaries;

3) Financials - summaries of 2021-2022 budget expenditures and fiscal year 2022-2023 budget; and

4) Media - website and social media information, samples of our Nebline newsletter and local newspaper articles.

Science Methods For Elementary Teachers, Ron Browne, David Kimori

Science Methods for Elementary Teachers

Complete Digitized Text of Science Methods for Elementary Teachers by Ron Browne and David Kimori, Minnesota State University, Mankato, 2022.

Undergraduate Research Projects: Step-By-Step, Elizabeth J. Sandell

Undergraduate Research Projects: Step-by-Step

This book is in a workbook format, intended to complement any individual or group of undergraduate researchers in their social science investigations. The step-by-step approach provides understanding and experience with scholarly inquiry. Students discover content and practice skills related to scholarly inquiry and their academic subjects.