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Tremblings, August 2021, Western Aspen Alliance

Tremblings

Knowing the WAA Through Connection

Paul C. Rogers

The Western Aspen Alliance (WAA) was founded in 2008 with a three-year start-up fund from Utah State University’s Quinney Foundation. Envisioned as a partnership between USU and the U.S. Forest Service’s research branch, the organization quickly attracted multiple partners in the form of state and federal agencies. Thus, a virtual organization was born with the primary function of bringing current aspen science to field practitioners. A secondary mission, to support active aspen research, involved facilitating interdisciplinary teams to address key issues. This basic structure, with science outreach overseen by a Steering Committee …

Crip Mothering: Representation Of Disability And Motherhood In Post-1980 American Films, Mohammed Ali H. Sumili

English Dissertations - Archive

Most scholarship on the topic of disability and motherhood tends to be of an empirical nature common to social, education, and psychology studies (Landsman 2008; Wilson and Cellio 2011, Filax and Taylor 2014). Despite the appearance of movies, novels and especially memoirs that deal with the subject, disability as it engages with motherhood has received scarce attention by feminist, motherhood, and disability scholarship (Prilleltensky 2004; Landsman 2008). Existing literature indicates a gap in studying the joint representation of mothers and disability. This study aims to address this gap by analysis of a selection of post-1980 American movies. Concentrating on the …

Twenty Years Of Land Use And The Impact Of Nitrate, E. Coli And Chlorophyll For Two Lakes In North Central Texas, Alicia Lee

Biology Theses - Archive

Clean and safe drinking water is a human right. Global, federal, and state agencies that monitor the source and quality of drinking water are limited in power and oversight. Historically, human groups have replaced wetland habitat with cropland, disregarding the abundance of resources. To mediate the loss, human constructed artificial wetlands aid in cleaning natural water ways. In chapter one, four floating wetland structures were installed on Lake Arlington and historical water quality data from 2001-2018 was analyzed. Historical data included six variables: Chlorophyll α, Dissolved Oxygen, Temperature, Specific Conductivity and Turbidity. The Mann Kendall Seasonality Trend test found that …

Determination Of The Kinetic Roles Of The Membrane In The Catalysis Of Rasgefs By Using An Engineered Model Protein, Hanh My Hoang

Chemistry & Biochemistry Dissertations - Archive

Ras guanine nucleotide exchange factors (RasGEFs), which include Son of Sevenless (SOS), Ras Guanine Nucleotide Releasing Protein (RasGRP), and Ras Guanine Nucleotide Releasing Factor (RasGRF), catalyze nucleotide exchange of Ras upon activating signals. Crystal structure analyses suggest that catalytic cavities of RasGEFs are not deep enough to uphold their substrates. Given that RasGEFs are all membrane binding proteins, a novel “membrane supplement hypothesis” is proposed that RasGEFs in cytoplasm are catalytically unformed; and RasGEFs on membrane are catalytically competent. The reason RasGEFs are catalytically unformed without the support of membrane is because the catalytic sites of RasGEFs are not sufficiently …

Studies On Enantioselective Desymmetrizing Alkyne Hydrosilylation, Yao Chung Chang

Chemistry & Biochemistry Theses - Archive

This research focuses on the design and synthesis of enantioselective desymmetrizing α-hydrosilylation of propargyl alcohols to provide α-hydroxy (E)-vinylsilanes. Transition metal-catalyzed, regio-and stereoselective intramolecular hydrosilylation of propargylic alcohols with an easily removable acetal directing group has been studied. This project thus far screened 35 chiral ligands with rhodium (I) to achieve enantioselective alkyne hydrosilylation. This strategy consists of sequential hydrosilylation of bis-propargyl formate with iridium catalyst and intramolecular alkyne hydrosilylation with chiral rhodium catalyst. To this end, a variety of chiral phosphine ligands has been tested, yet an enantiomeric excess of the α-hydroxy (E)-vinylsilanes has not been fruitful thus far, …

Chemical And Genetic Analysis Of The Mitochondrial Unfolded Protein Response, Mustafi Raisa Amin

Biology Dissertations - Archive

Mitochondria are essential for energy production, metabolic signaling, calcium homeostasis, and other roles. Consequently, mitochondrial dysfunction can result in cellular decline and the onset of disease. Cells use the mitochondrial unfolded protein response (UPRmt) to facilitate the recovery of damaged mitochondria to restore homeostasis. Recent studies have shown that the UPRmt plays a vital role in tumorigenesis and cancer survival. Therefore, UPRmt inhibition may be used as an anticancer strategy with great therapeutic results. Moreover, a known paradigm exists between mitochondrial stress signaling and the regulation of organismal lifespan. Since the UPRmt is activated during mitochondrial stress, it is believed …

Genomic Structure And Diversity In Neotropical Herpetofauna: The Role Of Demographic History And Gene Flow, Danielle George Rivera

Biology Dissertations - Archive

The effects of genetic introgression on species boundaries and how they affect species’ integrity and persistence over evolutionary time have received increased attention. The increasing availability of genomic data has revealed contrasting patterns of gene flow across genomic regions, which impose challenges to inferences of evolutionary relationships and of patterns of genetic admixture across lineages. By characterizing patterns of variation across thousands of genomic loci in a widespread complex of true toads (Rhinella), we assess the true extent of genetic introgression across species thought to hybridize to extreme degrees based on natural history observations and multi-locus analyses. Comprehensive geographic sampling …

Genetic Innovation Through Gene Duplication In Drosophila, Seyedeh Mirsalehi

Biology Dissertations - Archive

Among several mechanisms, whole gene duplication is one of the major sources for the generation of new genes. A large portion of young genes that emerged through whole gene duplication in Drosophila are specifically expressed in the testes and fast evolving, i.e., their proteins are changing very fast between species under positive selection. In this dissertation, we have studied the rates of duplications and functions of newly duplicated genes in three different projects to understand the evolutionary pressures/drivers of this gene innovation. Chapter 1 is an introductory section. In Chapter 2, by using a comprehensive, detailed phylogenomic study, we examined …

Investigating The Microevolutionary Processes That Promote And Maintain Biodiversity And Trait Novelty, Thomas Joseph Firneno

Biology Dissertations - Archive

Gene flow, mutation, selection, and genetic drift influence patterns of speciation, adaptation, and biodiversity. In the age of genomics, we have been increasingly able to understand the interplay between these microevolutionary processes at the genomic level, and their link to the phenotype and the environment. This, in turn, has provided clarity to the patterns of diversity and biological innovation that these processes generate. My dissertation examines how these microevolutionary processes have influenced patterns of diversity and the evolution of a novel trait in true toads (Anura: Bufonidae). First, I explore what processes (gene flow, incomplete lineage sorting, poor taxonomic resolution) …

Sex Differences And Vascular Mechanisms Of Elevated Cardiovascular Disease Risk In Non-Hispanic Black Individuals, John David Akins

Kinesiology Dissertations - Archive

Cardiovascular diseases (CVD), including hypertension, disproportionately affect the non-Hispanic black (BL) population in the United States. Despite being an inherently multifactorial process, altered vascular function remains a critical hallmark in the development of CVD. Further, blunted vascular function is often observed in the BL population. Recent advancements have linked several observational and mechanistic findings to the reduced vascular function in BL individuals, though the breadth of functional disparities in this population, the mechanistic contributors to these differences across sexes, and possible interventions to improve vascular function remain unelucidated. Therefore, this dissertation aimed to expand our current understanding of the vascular …

Per- And Polyfluorinated Alkyl Substances (Pfas) Cycling Within Michigan: Connections Between Contaminated Sites, Landfills, And Wastewater Treatment Plants, Ross W. Helmer

Masters Theses

The disposal and treatment of Per- and Polyfluorinated Alkyl Substances (PFAS) contaminated solid and liquid wastes, and associated cycling within the natural and engineered environment is a complex topic. PFAS data on contaminated sites, landfill leachates, the influent, effluent, and biosolids from wastewater treatment plants (WWTPs) within Michigan were compiled from publicly available and private sources. Most of the datasets are based on a 28-analyte suite generated using a modified form of the US EPA 537 analytical method, with a subset of data from the 18-analyte ASTM D7979-19 method. Approximately 70% of the 171 contaminated sites in Michigan have maximum …

Full Opioid Agonists And Tramadol: Pharmacological And Clinical Considerations, Amber N. Edinoff, Leah A. Kaplan, Sami Khan, Murray Petersen, Emily Sauce, Christopher D. Causey, Elyse M. Cornett, Farnad Imani, Omid Moradi Moghadam, Adam M. Kaye, Alan David Kaye

School of Pharmacy Faculty Articles

Opioids are mu receptor agonists and have been an important part of pain treatment for thousands of years. In order to use these drugs appropriately and successfully in patients, whether to control pain, to treat opiate-induced side effects, or opiate withdrawal syndromes, a solid understanding of the pharmacology of such drugs is crucial. The most recognized full agonist opioids are heroin, morphine, codeine, oxycodone, meperidine, and fentanyl. Phenanthrenes refer to a naturally occurring plant-based compound that includes three or more fused rings. The opioids derived from the opium plant are phenanthrene derivatives, whereas most synthetic opioids are simpler molecules that …

Modulation Of Glucose Homeostasis By Nucleotide P2y2 Receptor And Biological Sex, Hailee Anne Marino

Graduate Theses/Dissertations

Recent insights into the pathological role of Nucleotide P2Y₂ receptor suggest P2Y₂R involvement in high fat diet-induced obesity and potentiates insulin resistance. However, these recent insights do not demonstrate how P2Y₂R modulates glucose homeostasis under physiological conditions. Further, it remains unknown how sex biological factors influence P2Y₂R receptor signaling in the regulation of glucose homeostasis. The research objective for the present study is to elucidate the novel roles of P2Y₂ in fasting blood glucose and glucose tolerance (basal insulin sensitivity) under resting conditions in males and females. We expected that under physiological …

Marker-Assisted Selection To Determine The Introgression Of Rpv-3 Mediated Downy Mildew Resistance In 'Chambourcin' X 'Caberenet Sauvignon' Grapevine Population, Kavya Sri Tummala

Graduate Theses/Dissertations

Downy mildew caused by the fungus, Plasmopara viticola in the grapes is one of the major diseases affecting crop yield throughout the world. Breeding grapes via conventional methods for the downy mildew resistance will take approximately 25-30 years to fully evaluate and release new cultivars. Because of the long breeding cycle in woody plant species like grape, developing DNA markers linked to genes or quantitative trait loci (QTLs) for horticultural traits can greatly accelerate the breeding process and allow a much more accurate selection of progeny. This study aims to determine the presence of resistance in the 267 F1 genotypes …

Investigation Of The Interface Reactions Of Ion Selective Electrode Membranes Using Chromatographic And Spectroscopic Analyses Of Erbium(Iii) Tetraphenylporphyrin, Alexis Rae Miller

Graduate Theses/Dissertations

Ion selective electrodes (ISEs) are analytical sensors that monitor the interactions between an ionophore within a polymeric membrane and ions in various solutions. The sensitivity and selectivity of ISEs is directly related to the chemical components. One type of ionopohore utilized in ISEs are metalloporphyrins. Metalloporphyrins have unique binding characteristics, which make them useful in sensors. The polymeric membranes that are synthesized and used in ISEs consist of a metalloporphyrin complex, polyvinyl chloride, and ortho-nitrophenyl octyl ether. The membrane unique to this project contains erbium (III) tetraphenylporphyrin as the metalloporphyrin ionophore. Various analytical techniques were applied in the investigation …

Investigation Into Bacterial Impairment Of Greene And Polk County Water Systems, John C. Kincaid

Graduate Theses/Dissertations

Bacterial impairment of water systems is a major issue facing mankind. Bacteria that are introduced into a system have the potential to cause harmful diseases to wildlife and humans. In Greene and Polk counties, many water systems have become bacterially impaired over the years. Despite this, little is known about the contamination of known harmful bacteria in this region. To address this issue, I investigated the presence of known human pathogens across water systems in these two counties, many of which have displayed high levels of E. coli and fecal indicator microorganisms over many years. I used a high-throughput sequencing …

Corbicula Fluminea Effects On Survival, Growth, And Drift Of Juvenile Lampsilis Siliquoidea In Laboratory Exposures, Allison Nicole Sieja

Graduate Theses/Dissertations

The Asian clam, Corbicula fluminea, is an invasive species that is abundant and often co-occurs with native freshwater mussels. Corbicula is widely suspected of having negative effects on native mussels, but few studies have empirically tested this hypothesis. I used laboratory experiments to evaluate how adult Corbicula affected the survivorship, growth, and drift of juvenile Lampsilis siliquoidea. Survival and growth of newly metamorphosed mussels were tested in downwelling flow-through chambers with glass-bead substrate. Treatments were control (no clams), small adult clams, or large adult clams. After 28 days, large clams slightly but significantly reduced the number of juveniles …

Spatial Variability Of Nonpoint Source Yields In Ozark Highlands Watersheds Under Historical And Recent Land Use Conditions, Tyler J. Pursley

Graduate Theses/Dissertations

Many of the environmental problems facing communities today stem from historical as well as present land use disturbances related to agriculture, urbanization, and resource extraction. It is important to evaluate a range of land use and soil effects on nonpoint source (NPS) pollution to fully understand land use-water quality relationships. The Ozark Highlands region has undergone significant phases of land use change throughout its settlement history and is actively developing today (2020). This study used nonpoint nitrogen (TN), phosphorus (TP), and sediment (TSS) yields predicted by the US EPA’s Spreadsheet Tool for Estimating Pollutant Loads (STEPL) model to evaluate human …

How Competition Undermines Deterrence, Kayse Jansen

Graduate Theses/Dissertations

The re-emergence of great power competition has brought with it a U.S. government-wide initiative to reclaim and strengthen advantage and influence across all elements of national power. Competition is considered necessary to secure American interests and protect the existing liberal international order, as well as uphold deterrence by enhancing the nation’s ability to impose costs and deny benefits. This view, however, neglects a critical factor in deterrence: the cost of restraint, which reflects the acceptability of the status quo. Paradoxically, the more successful the nation is at “competition,” the less likely it may be in important deterrence situations. Successful diplomatic …

Physiological, Biochemical, And Metabolic Responses Of Wheat (Triticum Aestivum) Exposed To Perfluorooctanesulfonic Acid (Pfos), Polycarp Chidiebere Ofoegbu

Graduate Theses/Dissertations

The effects of perfluorooctanesulfonic acid (PFOS) on agricultural plants is a serious environmental concern that needs to be addressed. PFOS is a member of a class of organic chemical compounds known as per and polyfluoroalkyl substances (PFAS). These compounds are utility chemicals in household, commercial, and industrial items, but have become environmental contaminants of global concern. Full lifecycle and short-term exposure studies of wheat grown in soil amended with 0, 25, and 50 mg/kg PFOS were conducted to assess the impacts of PFOS on wheat. PFOS uptake analysis, physiological assays, elemental analysis, and metabolomics were carried out in the full …

Examining Health Inequity In Ancient Egypt, Samantha Rose Gonzalez

Graduate Theses/Dissertations

This thesis explores the history of medicine in ancient Egypt between the Middle and New Kingdoms, and offers a case study highlighting the use of religion and magic in healing and analyzing health inequity. I am interested in medical practices, treatments, diagnosis methods, and access to healthcare in the ancient world. I seek to bridge the gaps and help unify the knowledge surrounding ancient Egyptian medical practices and contribute to the studies in the history of medicine. I explore types of diseases that commonly affected the ancient Egyptians and how they integrated religion and magic into their understanding and treatment …

Nucleotide P2y₂ Receptor-Dependent Leukocyte-Endothelial Interaction, Spencer E. Thomas

Graduate Theses/Dissertations

Extracellular nucleotides (ATP, UTP) released from cells act on nucleotide receptors to promote vascular inflammation. Increased leukocyte-endothelial interaction is a hallmark of vascular inflammation. The nucleotide P2Y₂ receptor (P2Y₂R), activated by extracellular ATP≈UTP, plays a role in cardiovascular homeostasis and immune regulation. Moreover, accumulating evidence from studies in vitro and in vivo models have implicated the P2Y₂R in the inflammatory response significantly contributing to the progression and pathogenesis of asthma, atherosclerosis, sepsis, and ischemia. I hypothesized that P2Y₂R activation by UTP, an agonist of the receptor, increased leukocyte rolling and adhesion in the microvasculature from baseline. To test the hypothesis, …

Neuronal Migration In Developmental Hyperserotonmia: Assessment Of Vesicular Glutamate In The Raphe Nuclei, Trey M. Shupp

Graduate Theses/Dissertations

The neurotransmitter serotonin is involved in the early development of the central nervous system and the organization of neurons throughout the cerebral cortex and cerebellum. It is proposed that serotonin indirectly interacts with cells in the marginal zone of the cerebral cortex known as Cajal-Retizus (CR) cells. These cells secrete the extracellular matrix protein reelin, which is known for its role in neuronal organization and migration during early neural development. It has been observed that low levels of serotonin are associated with similarly low levels of reelin during development and have been reported to result in disorganization of neurons in …

Expression, Purification And Characterization Of The Human Cytochrome P450 Enzyme 4x1 (Cyp4x1) Using Substrate Binding Affinity, Metabolic Assays And Protein Crystallization, Olusegun Adeolu Idowu

Graduate Theses/Dissertations

The human cytochrome P450 enzyme 4X1 (CYP4X1) is among the “orphan” cytochrome P450 meaning there is much unknown about its structure, biological function, and regulation in literature study. Scientific work on this protein has primarily been on identifying its physiological functions and expression sites. A recent study found 2 substrates (arachidonic acid and anandamide) catalyzed by the enzyme. Expression in humans appears to be primarily in the brain, prostate, lungs, and neurovascular systems and research has linked the protein as a potential chemotherapy target in brain cancer. So far, only a few inhibitors have been identified. The goal …

Establishing Regenerative Pasture Systems Using Management Intensive Grazing In The Ozarks, Shelbi M. Mundy

Graduate Theses/Dissertations

Soil carbon and soil health are important topics relating to how climate change is impacting agriculture, and how agriculture can in turn impact climate change. The agriculture industry, particularly beef production, has a large opportunity to use conservation agriculture techniques, such as rotational grazing, to offset some of the industry’s impact on carbon emissions, erosion, water pollution, and other environmental issues. This study is the beginning of a long-term project exploring regenerative pasture systems in the Ozarks. The project takes place in a rotational grazing system with 12 paddocks. The objectives of this study are to characterize soil types by …

Improving Sight Reading Through Beginning Band Instruction, Joshua Earl Kohl

Masters Theses

Aural skills training needs to be included in the beginning band curriculum. Educators strive to help students reach their potential. At the same time, they continue to use method books that emphasize a visual approach to music education. To fully engage with music, students should not just see the music written on a page but should be able to hear it as well. Through aural skills training, such as audiation, students will be able to internalize and hear the music they see without the need for an audial stimulant. This study defines the current amount of aural training included in …

The Importance Of Sight-Singing To Ear-Training And Musicianship Of Neurodiverse Students, Courtney Marie Stinson

Masters Theses

This curriculum project aims to recognize the importance of teaching students music with ear-training. The goal is to identify the strengths and weaknesses of ear-training methods. This paper will review existing ear-training methods research. The literature will be reviewed regarding the biology of how listeners hear music, how geographic location influences elements of sound, how emotion influences communication, and why people like the music they like. The second chapter of this article discusses how singing on solfège creates stronger musicianship in all musicians as ear-training is essential for teaching music. Ear-training helps students recognize sounds and sonic events they have …

Domestic Chicken Diversity: Origin, Distribution, And Adaptation., Raman Akinyanju Lawal, O Hanotte

Faculty Research 2021

Chicken is the most numerous among the domesticated livestock species. Across cultures, religions, and societies, chicken is widely accepted with little or no taboo compared to other domestic animals. Its adaptability to diverse environmental conditions and demonstrated potential for breeding improvement provide a unique genetic resource for addressing the challenges of food security in a world impacted by climatic change and human population growth. Recent studies, shedding new knowledge on the chicken genomes, have helped reconstruct its past evolutionary history. Here, we review the literature concerning the origin, dispersion, and adaptation of domestic chicken. We highlight the role of human …

Diagnostics And Correction Of Batch Effects In Large-Scale Proteomic Studies: A Tutorial., Jelena Čuklina, Chloe H Lee, Evan G Williams, Tatjana Sajic, Ben C Collins, María Rodríguez Martínez, Varun S Sharma, Fabian Wendt, Sandra Goetze, Gregory R Keele, Bernd Wollscheid, Ruedi Aebersold, Patrick G A Pedrioli

Faculty Research 2021

Advancements in mass spectrometry-based proteomics have enabled experiments encompassing hundreds of samples. While these large sample sets deliver much-needed statistical power, handling them introduces technical variability known as batch effects. Here, we present a step-by-step protocol for the assessment, normalization, and batch correction of proteomic data. We review established methodologies from related fields and describe solutions specific to proteomic challenges, such as ion intensity drift and missing values in quantitative feature matrices. Finally, we compile a set of techniques that enable control of batch effect adjustment quality. We provide an R package, "proBatch", containing functions required for each step of …

Case 3847 – Simopithecus Oswaldi Andrews, 1916 (Currently Theropithecus Oswaldi; Mammalia, Primates, Cercopithecidae), Proposed Conservation By Reversal Of Precedence With Cynocephalus Atlanticus Thomas, 1884., Eric Delson, David M. Alba, Stephen R. Frost, Dagmawit Abebe Getahun, Christopher C. Gilbert

Publications and Research

The purpose of this application, under Articles 23.9.3 and 81.1 of the Code, is to conserve the usage of the species-group name Simopithecus oswaldi Andrews, 1916 by giving it precedence over its senior subjective synonym Cynocephalus atlanticus Thomas, 1884. Theropithecus is a common to dominant member of the extinct primate community across Africa after 4 million years ago (Jablonski & Frost, 2010) and often co-occurred with extinct humans (Hominini); fossils are also known rarely across Eurasia (Roberts et al., 2014). Most fossil samples are currently included in Theropithecus oswaldi (Andrews, 1916), which is often divided into chrono-geographic subspecies. Cynocephalus atlanticus …