Digital Commons Network^™

Plastic In Agricultural Soils And Its Biological Impacts, Olivia Mckay

Master of Science in Environmental Sciences and Management Projects

Agricultural plastic mulch reduces weeds, yields higher crop quality and quantity, and increases soil temperature, but it can also become a soil pollutant. The impact of agricultural plastic contamination on soil microbial activity remains poorly documented. To better understand how plastic pollution influences soil microbial decomposers, we sampled three farms in Watsonville, CA. Each site is characterized by large amounts of plastic contamination in the form of polyethylene mulch and polyvinyl chloride dripline. The fields contain marked amounts of macro- (and presumably micro-) plastic fragments primarily derived from PVC dripline and polyethylene mulch. We haphazardly collected 6" deep soil samples …

Soil Disturbance In Sierra Nevada Montane Meadow Following Lodgepole Pine Removal, Nicholas Cary

Master of Science in Environmental Sciences and Management Projects

Climate change and other anthropogenic stressors are driving conifer encroachment into meadow habitat. Encroachment, if ignored, can revert meadows back into dense forested habitat, negating meadow’s ecologic services (Durak et al. 2014). This research attempts to measure soil and stream habitat disturbances in Rock Creek meadow, located with Collins Pine Company land in Plumas County, California after clear-cut removal of encroaching lodgepole pine with mechanical machinery. Soil bulk density, ground cover transect data, and stream habitat conditions were monitored before (July 2019) and after (June 2021) restoration to measure changes in soil compaction, stream temperature, and surface disturbance (rutting/ tracks). …

Overcoming Stress, Hunger, And Pain: Cocaineand Amphetamine-Regulated Transcript Peptide's Promise, Willis K. Samson, Daniela Salvemini, Gina L.C. Yosten

Biological Sciences Faculty Research & Creative Works

Cocaine- and amphetamine-regulated transcript encodes an eponymous peptide, CARTp, which exerts diverse pharmacologic actions in the central and peripheral nervous systems, as well as in several endocrine organs, including pancreas. Here we review those diverse actions, the physiological relevance of which had remained unestablished until recently. With the identification of a CARTp receptor, GPR160, the physiologic importance and therapeutic potential of CARTp or analogs are being revealed. Not only is the CARTp-GPR160 interaction essential for the circadian regulation of appetite and thirst but also for the transmission of nerve injury-induced pain. Molecular approaches now are uncovering additional physiologically relevant actions, …

Regulation Of Antigenic Variation By Trypanosoma Brucei Telomere Proteins Depends On Their Unique Dna Binding Activities, Bibo Li Ph.D., Yanxiang Zhao

Biological, Geological, and Environmental Faculty Publications

Trypanosoma brucei causes human African trypanosomiasis and regularly switches its major surface antigen, Variant Surface Glycoprotein (VSG), to evade the host immune response. Such antigenic variation is a key pathogenesis mechanism that enables T. brucei to establish long-term infections. VSG is expressed exclusively from subtelomere loci in a strictly monoallelic manner, and DNA recombination is an important VSG switching pathway. The integrity of telomere and subtelomere structure, maintained by multiple telomere proteins, is essential for T. brucei viability and for regulating the monoallelic VSG expression and VSG switching. Here we will focus on T. brucei TRF and RAP1, two telomere …

Investigations In The Cellular And Molecular Biology Of Human Airway Mucociliary Tissue, Vincent Manna

Graduate School of Biomedical Sciences Theses and Dissertations

Our laboratory has integrated the use of a human-derived, in vitro model of airway mucociliary tissue. We isolate human nasal epithelial cells (HNECs) from the nasal mucociliary tissue of donors with a small brush and expand the airway progenitor cells in culture. The HNECs are then seeded onto semi-permeable transwell inserts. The inserts are in contact with the media in the lower chamber but don’t contain media in the upper chamber therefore the cells are exposed to the air while drawing nutrients from the media below, this is called the Air-Liquid Interface (ALI). HNECs cultured at the ALI initiate a …

The Relationship Between Vitamin D Intake, Sun Exposure, Handgrip Strength And Hg Alc Levels In College-Age Adults, Janelle Melancon

Master's Theses

Vitamin D is known for its importance to bone health and calcium metabolism and plays a role in insulin resistance. Recent research has recognized its role in insulin secretion, inflammatory response, and adipose tissue changes as possible reasons for its association with insulin resistance and cardiovascular disease. The CDC (2019) reported that 20% of adolescents are now living with prediabetes. Most individuals with prediabetes progress to type 2 diabetes within ten years. Because many studies have focused on adults of all ages, it is important to research young adults. This study aimed to determine prevention efforts; therefore, the younger adult …

The Role Of Notch1 And Notch3 In Adult Stem Cell Osteogenic Differentiation, John Bradley Cart

Master's Theses

Human adipose-derived stem cells (hASCs) have significant therapeutic potential due to their ability to self-renew, differentiate down multiple lineages, and modulate the immune system. In addition to these many benefits, hASCs boast a minimally invasive harvesting procedure, making them a readily available cell source for stem cell research and tissue regeneration (Ock, et al. 2016) (Abdi, et al. 2008). Despite their broad use, very little is known about the mechanisms that control cell fate.


One way to enhance our mechanistic understanding of differentiation is through the systematic examination of the signaling pathways. The Notch signaling pathway is a highly conserved, …

Exosense: A Novel Technology For Selective Purification Of Cd63+ Exosomes From Neural Cells, Kristen Hope Hutson Truelove

Master's Theses

Exosomes are extracellular, cell signaling microvesicles that contain unique genomic and proteomic signatures reflective of the host cell’s pathophysiological conditions. In recent years, the study of exosomes has increased tremendously because many have been recognized as molecular biomarkers with the potential to advance methods of disease diagnostics and therapeutics as well as contribute to physiological analyses of multiple organism types. With the promising potential that exosomes offer to the field of molecular biology, it is vital to establish an efficient and consistently reliable mechanism of exosome isolation from biological samples. Many isolation techniques currently available typically yield exosome samples with …

Imputation Of Missing Count Data Of Recreational Boat Retrievals From Remote Camera Surveys In The Perth Metropolitan Region, Western Australia, E. Afrifa-Yamoah, S.M Taylor, Cameron J. Desfosses, U. A. Mueller

Fisheries Research Reports

In Western Australia (WA), remote cameras have been used to monitor boating activity at boat ramps since 2006 to assist in ongoing recreational fishing surveys. These monitoring schemes are useful tools to validate boating activity (defined here as the number of retrievals) and to corroborate estimates of recreational fishing effort obtained from other surveys. This is because remote cameras (also referred to as digital cameras) can provide complete coverage (i.e. 24 hours, 365 days) of boating activities.

Elementary School Library Collections: A Content Analysis Of Science Trade Books, Sandra W. Watson, Sheila F. Baker

Reading Horizons: A Journal of Literacy and Language Arts

In this study, science trade books from the libraries of 10 elementary schools across the United States were evaluated using the modified Hunsader rubric for their overall quality pertaining to science content, literacy, and critical literacy criteria. Findings indicate that 62% of the books met the overall science content criterion, 99% met the overall literacy criterion, and 41% met the overall critical literacy criterion. The majority of science trade books in each school were life science books, and the majority of books across all schools were 18–23 years old, with many being much older. Implications and recommendations are provided.

Multi-Modal Data Fusion, Image Segmentation, And Object Identification Using Unsupervised Machine Learning: Conception, Validation, Applications, And A Basis For Multi-Modal Object Detection And Tracking, Nicholas Lahaye

Computational and Data Sciences (PhD) Dissertations

Remote sensing and instrumentation is constantly improving and increasing in capability. Included within this, is the increase in amount of different instrument types, with various combinations of spatial and spectral resolutions, pointing angles, and various other instrument-specific qualities. While the increase in instruments, and therefore datasets, is a boon for those aiming to study the complexities of the various Earth systems, it can also present a large number of new challenges. With this information in mind, our group has set our aims on combining datasets with different spatial and spectral resolutions in an effective and as-general-as-possible way, with as little …

Effects Of Trans-Acting Factors On The Translational Machinery In Yeast, Brandon M. Trainor

Graduate School of Biomedical Sciences Theses and Dissertations

Synthesis of proteins, or translation, is a complex biological process requiring the coordinated effort of numerous protein and RNA factors. Central to translation is the ribosome, a complex macromolecular complex consisting of both ribosomal RNA (rRNA) and ribosomal protein (r-protein). Ribosomes are essential and are one of the oldest and most abundant biomolecules across all forms of life. In addition to the ribosome, translation requires messenger RNA (mRNA), transfer-RNA conjugated to an amino acid (aa-tRNA), translation factors, and energy in the form of ATP and GTP. Translation universally occurs in four major stages, initiation, elongation, termination, and recycling, with initiation …

Bioavailable Silicon: Release Rate From Additives & Substrates, Mackenzie G. Dey, Jennifer Boldt, Bruce Bugbee

Hydroponics/Soilless Media

Liquid fertilization is often used to supply silicon (Si) (Boldt & Altland, 2021), but Si is minimally soluble. Media components that provide a steady release of Si are valuable.

There are many options for Si media amendments, but Si release rate is not well characterized.

Our objective was to quantify Si release from media components to achieve a steady-state Si release as mono-silicic acid (H₄SiO₄) over time.

Criteria For Selecting Female Superintendents In A Southern State, Melanie Soignier

Doctoral Dissertations

The purpose of this study was to ascertain which personal characteristics and professional skills differentiate female superintendents from school board presidents during the hiring process of a new superintendent. Input from practicing Louisiana public school superintendents and school board presidents was obtained in this quantitative study. This study’s significance was to identify personal characteristics and professional skills identified by current female superintendents to have been significant factors in their selection to the superintendency. Current research and literature reviewed and a reliable survey instrument obtained the desired information from the sample. The components of the survey were divided into two categories: …

Biopsychosocial Factors During The Perinatal Period: Risks, Preventative Factors, And Implications For Healthcare Professionals, Ashley J. Blount, Charmayne R. Adams, Ann L. Anderson-Berry, Corrine Hanson, Kara M. Schneider, Gurudutt Pendyala

Office of Health Promotion Scholarship

Women face risks to their wellbeing during the perinatal period of pregnancy. However, there is a dearth of information on perinatal risk factors within the biopsychosocial paradigm. Emphasis is often placed on biological components associated with pregnancy and women’s health. However, psychological and social determinants of health are integral during the perinatal period, and mental wellness is often a determinant for positive maternal and neonatal health outcomes. This article reviews risk factors of perinatal wellness (e.g., physical and nutritional concerns, trauma, discrimination, adverse childhood events) and highlights protective factors for women in their perinatal period. Healthcare professionals can support perinatal …

Muddying The Waters: The Need For More Clarity Under The Clean Water Act, Georgia D. Reid

Buffalo Environmental Law Journal

No abstract provided.

Anti-Fat Attitudes And Psychological Distress: The Role Of Psychological Flexibility Among Stigmatizers, Emily Robin Squyres

Doctoral Dissertations

As the population of people struggling with obesity has grown, so has the discrimination and stigmatization of obese individuals. Psychological flexibility has been found to partially mediate the relationship between stigmatizing attitudes and psychological distress for those who hold stigmatizing attitudes toward individuals struggling with mental illness, such that high stigmatizing attitudes about individuals with psychological disorders is associated with low psychological flexibility and low psychological flexibility is associated with high psychological distress. Currently there is no research in the extant literature regarding the relationship between anti-fat attitudes, psychological distress, and psychological flexibility. As such, the present study examined the …

The Age Presents Introduction To Geroscience Video Lecture Series., Alessandro Bitto, Mitchell B Lee, Cristal M Hill, Ron Korstanje, Matt Kaeberlein

Faculty Research 2021

The AGE Presents Introduction to Geroscience video lecture series is a collection of high-quality didactic video lectures and associated teaching materials focused on foundational topics in aging biology. The videos are made freely available on YouTube and are targeted toward an audience familiar with concepts learned in the first year of a college undergraduate biology/biomedical major. Members of the American Aging Association also receive the original lecture slides and lecture notes, with additional course materials to be developed in the future. We expect that these lectures will enhance understanding of geroscience among the general public while also providing tools that …

Gene Expression Profiles Compared In Environmental And Malnutrition Enteropathy In Zambian Children And Adults, Paul Kelly, Beatrice Amadi, Kanta Chandwe, Ellen Besa, Kanekwa Zyambo, Mubanga Chama, Phillip I Tarr, Nurmohammad Shaikh, I. Malick Ndao, Chad Storer, Richard Head

Open Access Publications

BACKGROUND: Environmental enteropathy (EE) contributes to growth failure in millions of children worldwide, but its relationship to clinical malnutrition has not been elucidated. We used RNA sequencing to compare duodenal biopsies from adults and children with EE, and from children with severe acute malnutrition (SAM), to define key features of these malnutrition-related enteropathies.

METHODS: RNA was extracted and sequenced from biopsies of children with SAM in hospital (n=27), children with non-responsive stunting in the community (n=30), and adults living in the same community (n=37) using an identical sequencing and analysis pipeline. Two biopsies each were profiled and differentially expressed genes …

Clemson Commencement Program, August 2021, Clemson University

Clemson Commencement Programs

No abstract provided.

An Examination Of Chinese Undergraduates’ Contemplativity And Academic Stress, Ran Tao

Education (PhD) Dissertations

China has advanced considerably in many fields, which has led to fierce competition among its people. College students are no exception as this competition has brought tremendous academic stress that affects academic performance. Western scholars and researchers found contemplative practice (CP) was helpful to reduce students’ educational stress and improve their study performance. However, no literature was found regarding the potential impact of CP on undergraduates in China, nor was any literature discovered concerning potential differences between genders or academic emphases on students’ conceptions of CP and collegiate stress. The purpose of this study was to fill the gap by …

Empathy Among Nursing Undergraduates At A Chinese Private University, Yuanyuan Zhang

Education (PhD) Dissertations

Empathy is an indispensable professional quality in nursing. It correlates positively with the quality of caring, the nurse-patient relationship, patient satisfaction, and the outcomes of treatment. Understanding and cultivating empathy of nursing undergraduates has been one of the main focuses of nursing education. Taking into account the lack of quantitative research into the empathy of nursing undergraduates at China’s private colleges and the inconsistencies in the current literature in terms of assessments of the empathy levels of nursing undergraduates, this study used the Interpersonal Reactivity Index scale to examine the empathy levels of 644 nursing undergraduates at a private university …

Incorporation Of Glycyl-L-Histidyl-L-Lysine (Ghk) With Metalized Halloysites For Tissue Regeneration, Christopher Cameron Miller

Doctoral Dissertations

Human adipose stem cells (hASCs) provide a research tool for regenerative medicine in various capacities. There are major research needs for new therapies for bone-related diseases such as osteoporosis, osteomyelitis, and severe trauma. Human ASCs can self-renew for extended durations outside of the body and provide an ideal platform to study therapeutics for the formation of bone-specific modalities or wound healing applications.

Combining hASCs with nanomaterials is a growing area of research that requires additional studies. Halloysites (HNTs) are hollow aluminosilicate nanotubes with an exterior negative charge and a positively charged lumen. HNTs have demonstrated biocompatibility in cell and animal-based …

In Vivo And In Vitro Expression And Interaction Of The Mediator Kinase Module Subunits During Adipogenesis, Sree Lakshmi Venigalla

Doctoral Dissertations

Selective gene expression is crucial in maintaining the self-renewing and differential potential of stem cells. Especially differentiation process requires a constellation of proteins that regulate expression of genes necessary for differentiation towards a specific lineage. Adipogenesis is a process of formation of mature fat cells from precursor cells that is tightly regulated by a number of transcription factors and their cofactors. Dysregulation in the process often leads to metabolic disorders, which is common due to prevalence of obesity. Mediator is a large, evolutionarily conserved, multi-subunit protein complex that functions as a transcriptional coactivator that modulates gene expression by relaying signals …

College Of Natural Sciences Newsletter, August 2021, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 2, Issue 8.

Page 1 Dean's Message
Page 2 Awards and Recognition
Page 3 Student Summer Research
Page 4 Media Coverage of CNS
Page 5 Wintrode Student Success Center
Page 6-7 Welcome to our new Faculty & Staff!
Page 8 One Day for State: Thursday, Sept. 2nd
Page 7 Open PRAIRIE Data; Fall Faculty Retreat 2021

Mechanical Properties Of The High Cholesterol-Containing Membrane: An Afm Study, Nawal K. Khadka, Raju Timsina, Erica L. Rowe, Matthew O'Dell, Laxman Mainali

Physics Faculty Publications and Presentations

Cholesterol (Chol) content in most cellular membranes does not exceed 50 mol%, only in the eye lens’s fiber cell plasma membrane, its content surpasses 50 mol%. At this high concentration, Chol induces the formation of pure cholesterol bilayer domains (CBDs), which coexist with the surrounding phospholipid-cholesterol domain (PCD). Here, we applied atomic force microscopy to study the mechanical properties of Chol/phosphatidylcholine membranes where the Chol content was increased from 0 to 75 mol%, relevant to eye lens membranes. The surface roughness of the membrane decreases with an increase of Chol content until it reaches 60 mol%, and roughness increases with …

A Migratory Sparrow Has Personality In Winter That Is Independent Of Other Traits, Theadora A. Block, Rachel Star, Daizaburo Shizuka, Alexis S. Chaine, Bruce E. Lyon

Papers in Behavior in Biological Sciences

Small birds in winter face trade-offs between predation risk and foraging, and alternate life-history strategies may arise from these trade-offs. Animal personality shows similarities with alternative life-history strategies, and using a life-history context to understand personality can provide valuable insights. Golden-crowned sparrows, Zonotrichia atricapilla, a small migratory bird, have a complex winter social system with high site-fidelity, long-term social associations between individuals and competition mediated by badges of status. We asked whether golden-crowned sparrows show personalities during winter, whether these personalities were consistent over 3 years and whether they correlated with social and morphological traits. We found that …

Cytokines: From Clinical Significance To Quantification, Chao Liu, Dewei Chu, Kourosh Kalantar-Zadeh, Jacob George, Howard A. Young, Guozhen Liu

United States Public Health Resources

Cytokines are critical mediators that oversee and regulate immune and inflammatory responses via complex networks and serve as biomarkers for many diseases. Quantification of cytokines has significant value in both clinical medicine and biology as the levels provide insights into physiological and pathological processes and can be used to aid diagnosis and treatment. Cytokines and their clinical significance are introduced from the perspective of their pro- and anti-inflammatory effects. Factors affecting cytokines quantification in biological fluids, native levels in different body fluids, sample processing and storage conditions, sensitivity to freeze-thaw, and soluble cytokine receptors are discussed. In addition, recent advances …

Mental Healthcare In Costa Rica: A Critical Perspective, Claire Elise Decelles

Theses and Graduate Projects

The country of Costa Rica often ranks high on happiness metrics and healthcare quality metrics. Mental healthcare in Costa Rica is often not discussed when reviewing those metrics. This paper attempts to explore mental healthcare in Costa Rica through a critical lens. Specifically, what factors affect the mental health of Costa Rican’s, if they experience mental health issues at the same rates as other areas of the world, what access is like, the prevalence of stigma surrounding mental illness, and how the COVID-19 pandemic has affected mental health. Through literature review and conversing with locals, it was found that mental …

A Literature Review: What Mental Health Challenges Are Experienced By First-Generation Latino Immigrant And Refugee Families Entering The U.S.?, Hunnaan M. Khan

Theses and Graduate Projects

It is believed that people leave their homeland and come to America for better opportunities, freedom, safety, or second chances at life. As of 2021, Latino’s account for Americas largest minority population however measures to support their communities medically and socially have fallen short. The downfalls of misunderstanding the traumas experienced in their homelands, accounting for premigration, during migration, and postmigration stressors, and systemic issues that fall short in providing culturally sensitive care creates room for mental health challenges to accumulate. Cases of traumas experienced in homelands, cultural implications, the controversies, and difficulties of being held in detention centers, challenges …