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Unbiased Automated Quantitation Of Ros Signals In Live Retinal Neurons Of Drosophila Using Fiji/Imagej, Prajakta Deshpande, Neha Gogia, Anuradha Venkatakrishnan Chimata, Amit Singh

Biology Faculty Publications

Numerous imaging modules are utilized to study changes that occur during cellular processes. Besides qualitative (immunohistochemical) or semiquantitative (Western blot) approaches, direct quantitation method(s) for detecting and analyzing signal intensities for disease(s) biomarkers are lacking. Thus, there is a need to develop method(s) to quantitate specific signals and eliminate noise during live tissue imaging. An increase in reactive oxygen species (ROS) such as superoxide (O2•-) radicals results in oxidative damage of biomolecules, which leads to oxidative stress. This can be detected by dihydroethidium staining in live tissue(s), which does not rely on fixation and helps prevent stress on tissues. However, …

Enhancing Our Understanding Of Plant Cell-To-Cell Interactions Using Single-Cell Omics, Sandra Thibivilliers, Marc Libault

Department of Agronomy and Horticulture: Faculty Publications

Plants are composed of cells that physically interact and constantly adapt to their environment. To reveal the contribution of each plant cells to the biology of the entire organism, their molecular, morphological, and physiological attributes must be quantified and analyzed in the context of the morphology of the plant organs. The emergence of single-cell/nucleus omics technologies now allows plant biologists to access different modalities of individual cells including their epigenome and transcriptome to reveal the unique molecular properties of each cell composing the plant and their dynamic regulation during cell differentiation and in response to their environment. In this manuscript, …

Tandem Duplicate Expression Patterns Are Conserved Between Maize Haplotypes Of The Α-Zein Gene Family, Preston Hurst, James C. Schnable, David R. Holding

Department of Agronomy and Horticulture: Faculty Publications

Tandem duplication gives rise to copy number variation and subsequent functional novelty among genes as well as diversity between individuals in a species. Functional novelty can result from either divergence in coding sequence or divergence in patterns of gene transcriptional regulation. Here, we investigate conservation and divergence of both gene sequence and gene regulation between the copies of the α-zein gene family in maize inbreds B73 and W22. We used RNA-seq data generated from developing, self-pollinated kernels at three developmental stages timed to coincide with early and peak zein expression. The reference genome annotations for B73 and W22 were modified …

Identifying Transfer Of Care Gaps: Electronic Health Record Capture Of Perioperative Handoff Communications, Sharon Giarrizzo-Wilson

Dissertations

Transitions in patient care are held together by interdisciplinary handoff communications intended to coordinate the patient's ongoing care requirements. Patients with complexity in care encumber the transfer of care process requiring a higher level of care coordination between the interdisciplinary team (Coleman, 2003; Naylor et al., 2004). While the literature is abundant on the characteristics and quality of handoff communications, it is limited on the requirements of what data is necessary for ongoing care following transfer communications (Galatzan & Carrington, 2018).

Bottom Current Control On Sediment Deposition Between The Iselin Bank And The Hillary Canyon (Antarctica) Since The Late Miocene: An Integrated Seismic- Oceanographic Approach, R. Conte, M. Rebesco, Santis L De, F. Colleoni, M. Bensi, A. Bergamasco, V. Kovacevic, J. Gales, F. Zgur, D. Accettella, Steur L De, L. Ursella, R. Mckay, S. Kim, R. G. Lucchi

School of Biological and Marine Sciences

No abstract provided.

Detection Of Subclinical Rheumatic Heart Disease In Children Using A Deep Learning Algorithm On Digital Stethoscope: A Study Protocol, Fatima Ali, Babar Hasan, Huzaifa Ahmad, Zahra Hoodbhoy, Zainab Bhuriwala, Muhammad Hanif, Shahab U. Ansari, Devyani Chowdhury

Department of Paediatrics and Child Health

Introduction: Rheumatic heart diseases (RHDs) contribute significant morbidity and mortality globally. To reduce the burden of RHD, timely initiation of secondary prophylaxis is important. The objectives of this study are to determine the frequency of subclinical RHD and to train a deep learning (DL) algorithm using waveform data from the digital auscultatory stethoscope (DAS) in predicting subclinical RHD.
Methods and analysis: We aim to recruit 1700 children from a group of schools serving the underprivileged over a 12-month period in Karachi (Pakistan). All consenting students within the age of 5-15 years with no underlying congenital heart disease will be eligible …

Endophytic Beauveria Bassiana Increases Galling Of ‘Rutgers’ Tomato Roots With Meloidogyne Incognita, Shalini Yerukala, Ernest C. Bernard, Kimberly D. Gwinn, David M. Butler, Parwinder Grewal, Bonnie H. Ownley

School of Earth, Environmental, & Marine Sciences Faculty Publications

Beauveria bassiana is endophytic in many plant species and has been shown to protect host plants against insect pests and plant pathogens. However, less is known about its activity against plant-parasitic nematodes. In vitro and plant assays were conducted to determine the effect of B. bassiana 11-98 (Bb) on Meloidogyne incognita (root-knot nematode; RKN). Beauveria bassiana was confirmed as an endophyte in ‘Rutgers’ tomato and colonization patterns of Bb in ‘Rutgers’ (highly susceptible to RKN) were compared with those in ‘Mountain Spring’ (less susceptible to RKN). In greenhouse tests with ‘Rutgers’ at 30 and 60 days after treatment (DAT) with …

Contraception For Pcps 2021, Krys Foster, Md, Mph, Faafp

Department of Family & Community Medicine Presentations and Grand Rounds

Objectives:

• Review evidence-based, flexible and patient-centered approaches to contraceptive management in individuals with reproductive potential
• Discuss updates in contraceptive prescribing and management
• Highlight considerations for management of contraception in the time of COVID-19 and in special populations

Outline

• Introduction
• Approach to initiation of contraception
• Overview of methods of contraception
• Special Considerations
• Resources (Medical Eligibility and Special Practice Recommendations; Contraception App)
• Summary
• References

Urinary Minerals Excretion Among Primary Schoolchildren In Dubai—United Arab Emirates, Rola Al Ghali, Carla El-Mallah, Omar Obeid, Ola El-Saleh, Linda Smail, Dalia Haroun

All Works

INTRODUCTION: Urinary excretion of calcium (Ca), magnesium (Mg), phosphorus (P), iodine and fluoride is used to assess their statuses and/or the existence of metabolic abnormalities. In the United Arab Emirates (UAE), the urinary concentration of these minerals among children have not been documented. MATERIALS AND METHODS: A cross-sectional study, including 593 subjects (232 boys and 361 girls), was conducted among healthy 6 to 11-year-old Emirati children living in Dubai. Non-fasting morning urine samples and anthropometrical measurements were collected and analyzed. Results were expressed as per mg of creatinine (Cr). RESULTS: On average, estimated Cr excretion was 17.88±3.12 mg/kg/d. Mean urinary …

Immune Cell Residency In The Nasal Mucosa May Partially Explain Respiratory Disease Severity Across The Age Range., Konner Winkley, Dithi Banerjee, Todd Bradley, Boryana Koseva, Warren A. Cheung, Rangaraj Selvarangan, T Pastinen, Elin Grundberg

Manuscripts, Articles, Book Chapters and Other Papers

Previous studies focusing on the age disparity in COVID-19 severity have suggested that younger individuals mount a more robust innate immune response in the nasal mucosa after infection with SARS-CoV-2. However, it is unclear if this reflects increased immune activation or increased immune residence in the nasal mucosa. We hypothesized that immune residency in the nasal mucosa of healthy individuals may differ across the age range. We applied single-cell RNA-sequencing and measured the cellular composition and transcriptional profile of the nasal mucosa in 35 SARS-CoV-2 negative children and adults, ranging in age from 4 months to 65 years. We analyzed …

Molten-Salt-Assisted Annealing For Making Colloidal Znga2o4:Cr Nanocrystals With High Persistent Luminescence, Bhupendra B. Srivastava, Santosh K. Gupta, Swati Mohan, Yuanbing Mao

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)

Persistent luminescent nanocrystals (PLNCs) in the sub-10 nm domain are considered to be the most fascinating inventions in lighting technology owing to their excellent performance in anti-counterfeiting, luminous paints, bioimaging, security applications, etc. Further improvement of persistent luminescence (PersL) intensity and lifetime is needed to achieve the desired success of PLNCs while keeping the uniform sub-10 nm size. In this work, the concept of molten salt confinement to thermally anneal as-synthesized ZnGa2O4:Cr3+ (ZGOC) colloidal NCs (CNCs) in a molten salt medium at 650 °C is introduced. This method led to significantly monodispersed and few agglomerated NCs with a much improved …

An End-To-End Cnn With Attentional Mechanism Applied To Raw Eeg In A Bci Classification Task, Elnaz Lashgari, Jordan Ott, Akima Connelly, Pierre Baldi, Uri Maoz

Psychology Faculty Articles and Research

Objective. Motor-imagery (MI) classification base on electroencephalography (EEG) has been long studied in neuroscience and more recently widely used in healthcare applications such as mobile assistive robots and neurorehabilitation. In particular, EEG-based motor-imagery classification methods that rely on convolutional neural networks (CNNs) have achieved relatively high classification accuracy. However, naively training CNNs to classify raw EEG data from all channels, especially for high-density EEG, is computationally demanding and requires huge training sets. It often also introduces many irrelevant input features, making it difficult for the CNN to extract the informative ones. This problem is compounded by a dearth of training …

Bibliometric Study Of Masters’ And Ph. D. Theses Accepted By Indian State Agricultural Universities (Saus) In 2018, Kailash Chandra Garg Dr, Sonia Bansal Ms

Library Philosophy and Practice (e-journal)

An analysis of 5,703 theses accepted by 54 Indian State Agricultural Universities in 2018 and indexed by Krishikosh, a digital repository of Indian Council of Agricultural Research (ICAR) indicates that male scholars produced more than half of the theses at different levels of education, i.e. M.Sc., M.V. Sc., M.B.A., M. Tech and Ph.D. However, at M. Tech level, female scholars produced more theses than their male counter parts. Highest number of theses was produced by Govind Ballabh Pant University of Agriculture and Technology (Pant Nagar) followed by Punjab Agriculture University (Ludhiana). Agronomy and soil science was the area emphasized by …

A Patient’S Narrative Of Engaging Hiv Care: Lessons Learned To Harness Resources And Improve Access To Care, David Lessard, Serge Vicente, Patrick Keeler, Bertrand Lebouché

Patient Experience Journal

In Canada and the USA, about 30% of people with HIV are uninsured or not covered by government-subsidized health insurance. This paper presents a patient’s narrative of his experience being diagnosed with HIV and accessing care in the midst of his process of immigrating to and studying in Canada. The narrative explores how Vincent Croft (pseudonym) has coped with the chronicity of the infection and its associated social stigma, and the temporary solutions he found to access treatment. Engaging with healthcare providers, researchers, and other people living with HIV has allowed Croft to share his experience, including the barriers he …

Students And Farmers Planning And Implementing Grass Based Agroecologic Dairy Systems As A Regular Statewide Program: The Development Of Participative Methodology Responsible For 622 Family Farm Projects, W. Murphy, A. Schmitt, F. Busnardo, J. Ross, L. Assing, A. Nacimento

IGC Proceedings (1977-2023)

No abstract provided.

Protecting Wildlife Habitat On Ranchettes, L. J. Kennedy, Ken Strom, Jacelyn Downey, Alison Lyon‐Holloran, Robert Petty

IGC Proceedings (1977-2023)

No abstract provided.

Strategies For Sustaining Multiple Ecosystem Services From Rangelands, Kris M. Havstad, J. R. Brown, J. E. Herrick, D. P. C. Peters, E. L. Fredrickson, Brandon T. Bestelmeyer, R. Pieper

IGC Proceedings (1977-2023)

No abstract provided.

Total Flavonoid Content, Total Phenolic Content, Antimicrobial And Antioxidant Activities Of Acacia Rigidula Leaf Extracts, Pablo Arenaz

Theses and Dissertations

The growing incidence of multidrug resistant bacteria has prompted a need for alternatives to traditional antibiotics. The Fabaceae family including the Acacia species have been reported to contain secondary metabolites known to exhibit antibacterial and antioxidant activities. Specifically, A. ridigula was previously shown to be effective against a range of bacterial species. When extracting secondary metabolites from plants, the method and choice of solvent is important in order to efficiently extract a high yield. This study aims to: 1) determine whether Soxhlet or maceration is the more efficient extraction method; 2) determine whether acetone and ethanol will yield more bioactive …

Fast Multipole Methods For Wave And Charge Source Interactions In Layered Media And Deep Neural Network Algorithms For High-Dimensional Pdes, Wenzhong Zhang

Mathematics Theses and Dissertations

In this dissertation, we develop fast algorithms for large scale numerical computations, including the fast multipole method (FMM) in layered media, and the forward-backward stochastic differential equation (FBSDE) based deep neural network (DNN) algorithms for high-dimensional parabolic partial differential equations (PDEs), addressing the issues of real-world challenging computational problems in various computation scenarios.

We develop the FMM in layered media, by first studying analytical and numerical properties of the Green's functions in layered media for the 2-D and 3-D Helmholtz equation, the linearized Poisson--Boltzmann equation, the Laplace's equation, and the tensor Green's functions for the time-harmonic Maxwell's equations and the …

Science Daily Reports On Rothwell's Two New Genera Of Ancient Conifers From Upper Cretaceous, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

Biochemical Evaluation Of Drug Inhibition Of Abc Transporters, Maisa Correa De Oliveira

Biological Sciences Theses and Dissertations

Multidrug resistant (MDR) cancers often become resistant to pharmacologically and structurally diverse drugs by overexpressing members of the family of ATP Binding Cassette (ABC) transporters. These membrane pumps protect normal cells by actively exporting a large range of toxins out of the cells. Classic MDR is often correlated with the overexpression of three well-studied mammalian ABC transporters: P-glycoprotein (P-gp), Breast Cancer Resistant Protein (BCRP), and Multidrug Resistant Protein 1 (MRP1).

P-gp is the most studied ABC transporter, and the search for P-gp inhibitors as co-therapeutics to combat MDR has had little success after decades of studies. We previously used high-throughput …

The Zooarchaeological Dimension Of Preceramic Human-Environment Dynamics In The Highlands Of Southwestern Honduras, Alejandro J. Figueroa

Anthropology Theses and Dissertations

In this dissertation I evaluate different hypotheses regarding human-environment dynamics in the Mesoamerican neotropics during the Preceramic period (ca. 11000-7400 cal B.P.) by examining the largest extant faunal assemblage dated to this time. The Preceramic was characterized by major climatic and ecological changes following the end of the Pleistocene, including the extinction of megafauna and the expansion of tropical forests. This period ended with a series of behavioral adaptations suited to this transformed landscape such as increased territoriality, sedentism, agriculture, and domestication. Three hypotheses have been proposed to explain these dynamics: the Broad-Spectrum Revolution hypothesis suggests post-Pleistocene resource uncertainty and …

Graduate Students Awarded Amc21 Program Scholarship To Advance Biomedical Research, Staff

Browse All News

No abstract provided.

Quantitative Soundscape Analysis To Understand Multidimensional Features, Dylan Charles Wilford, Jennifer L. Miksis-Olds, S. Bruce Martin, Daniel R. Howard, Kim Lowell, Anthony P. Lyons, Michael James Smith

Center for Coastal and Ocean Mapping

A methodology for the analysis of soundscapes was developed in an attempt to facilitate efficient and accurate soundscape comparisons across time and space. The methodology consists of a collection of traditional soundscape metrics, statistical measures, and acoustic indices that were selected to quantify several salient properties of marine soundscapes: amplitude, impulsiveness, periodicity, and uniformity. The metrics were calculated over approximately 30 h of semi-continuous passive acoustic data gathered in seven unique acoustic environments. The resultant metric values were compared to a priori descriptions and cross-examined statistically to determine which combination most effectively captured the characteristics of the representative soundscapes. The …

Gardner-Webb Begins New Bachelor Of Science Degree In Biochemistry, Office Of University Communications

Gardner-Webb NewsCenter Archive

Gardner-Webb University offers a new undergraduate degree in biochemistry, beginning this fall. The Bachelor of Science program meets the needs of students who plan to pursue graduate degrees for health-related fields or biomedical research. Students could also go directly into jobs with chemical or pharmaceutical companies.

The Inconspicuous Lives Of Dr. Susan Smith Mckinney Steward And Dr. Elizabeth Blackwell, Alexandra W. Bogdanovich

Theses and Dissertations

This paper examines trailblazing American female doctors of the nineteenth century in New York. Through the lives of Dr. Susan Smith Mckinney Steward, who is black, and Dr. Elizabeth Blackwell, who is white, this analysis tries to understand what motivated these women and how they succeeded in spite of the confines of women’s prescriptive role in nineteenth-century America.

The Role Of Hydrogen Bonding And Hydrogen Migration In Different Environments, Nassim Beiranvand

Chemistry Theses and Dissertations

Hydrogen bonding is one of the most important topics in chemistry and has been a subject of many studies for years, using both experimental techniques and computational methods. My dissertation focuses on mechanistic studies of various types of intermolecu-lar and intramolecular hydrogen bonding in di˙erent systems based on quantum chemical calculations. In my work, I investigated the interplay of ring puckering and hydrogen bonding in DNA building blocks; hydrogen bonding in natural and unnatural base pairs and the nature of dihydrogen bonding by applying the Ring Puckering Analysis and the Local Vibrational Mode Theory (LVM). An additional aim of my …

Synthesis Of P-Glycoprotein Inhibitor Compounds, Maha Aljowni

Chemistry Theses and Dissertations

SYNTHESIS OF P-GLYCOPROTEIN INHIBITOR COMPOUNDS Advisor: Professor Alexander R. Lippert Doctor of Philosophy conferred: August 4th, 2021 Dissertation completed: August 4th, 2021 A problem with cancer treatment is that many cancers develop resistance to chemotherapeutic agents, causing them to fail to accumulate in resistant cancer cells long enough to have any effect. This is due to the overexpression of a plasma membrane protein called P-glycoprotein (P-gp). Generally, the role of P-gp is to protect the cells from any toxins or foreign substances by pumping these toxins (including chemotherapeutic drugs) out of the cell. I am collaborating with the Wise-Vogel laboratory …

Application Of Artificial Intelligence And Machine Learning In Chemistry, Niraj Verma

Chemistry Theses and Dissertations

In the last four years, I have been exposed to various topics in scientific research under the supervision of Dr. Kraka in the CATCO group. Numerous involved chemistry projects were undertaken to gain an understanding of the basic laws of nature involving vibrational spectroscopy, molecular acidity, and catalysts based on transition metals for halogen chemistry. The insights from computational chemistry were then applied to model and predict various complicated problems in chemistry via artificial intelligence. With the help of classical artificial intelligence, the non-covalent interactions governing the properties of proteins and water properties were analyzed. Significant improvements were made in …

The Origin Of Ticks And The Genesis And Emergence Of Lyme Disease, Alan L. Gillen, Matthew Eakin

Faculty Publications and Presentations

Ticks are notorious for their capability of transmitting diseases that have debilitated humanity for thousands of years. They can confer lifelong chronic ailments via pathogenic bacterial species that they harbor inside themselves. These parasitic tick species, namely the Ixodes scapularis tick is responsible for hundreds of thousands of Lyme disease infections each year. This profound tick continues to produce the highest rate of zoonotic disease in the United States each year. Many may ask why these tick species are so infectious when ticks were originally designed by a benevolent God. Why would a good Creator choose to design a creature …