Digital Commons Network^™

Metabolic Control And Immune Barriers Of Hematopoietic Stem Cells, Hannah Pizzato

Arts & Sciences Graduate Student Theses and Dissertations

Hematopoietic stem cells (HSCs) have the unique ability to self-renew for life, to differentiate into mature blood lineages, and to readily engraft upon intravenous transplantation. As such, they are the only types of stem cells in routine clinical use. Understanding HSCs and hematopoietic development can provide many lessons for other types of stem cells as they near clinical utility. Through bone marrow transplantation, it was discovered that cells exist with regenerative potential. This led to the search to purify these cells and to determine the function of other hematopoietic cells. By isolating and transplanting cells expressing different combinations of surface …

Regulation Of Host-Microbe Interactions In Autoimmunity And Antiviral Immunity By Cytosolic Nucleic Acid Sensing And Interferon Signaling, Derek Jerome Platt

Arts & Sciences Graduate Student Theses and Dissertations

Cytosolic nucleic acid sensing and interferon (IFN) signaling are central to the host immune response to microbial pathogens. However, dysregulation of immunological pathways such as these can result in devastating autoimmune disease. In order to provide a robust immune response to pathogen without causing harm to self, the host immune system must engage in a delicate balancing act, interacting with microbes and determining whether they are commensal or pathogenic. The cGAS-STING pathway is a key regulator of host-microbe interactions by cytosolic nucleic sensing and IFN signaling. Loss of function in the cGAS-STING pathway leads to increased susceptibility to pathogenic threats, …

Computational Approaches For Screening Drugs For Bioactivation, Reactive Metabolite Formation, And Toxicity, Noah Flynn

Arts & Sciences Graduate Student Theses and Dissertations

Cytochrome P450 enzymes aid in the elimination of a preponderance of small molecule drugs, but can generate reactive metabolites that may adversely conjugate to protein and DNA, in a process known as bioactivation, and prompt adverse reaction, drug candidate attrition, or market withdrawal. Experimental assays are low-throughput and expensive to perform, so they are often reserved until later stages of the drug development pipeline when the drug candidate pools are already significantly narrowed. Reactive metabolites also elude in vivo detection, as they are transitory and generally do not circulate. In contrast, computational methods are high-throughput and cheap to screen millions …

Impact Of Care Practices On The Food Security And Nutritional Status Of Ethnic Minority Children With Work-Away Parents In Rural China, Yiqi Zhu

Arts & Sciences Graduate Student Theses and Dissertations

Background and significance: Zero Hunger as the second Sustainable Development Goal builds crucial foundation to achieve other sustainable development goals and is a key approach to the Social Work Grand Challenges. In China, there is a huge gap between in nutritional well-being between urban and rural children. Among all the rural Children, ethnic minority children often live in the poverty-stricken regions have parents work away in urban regions. Their nutritional well-being is understudied. Improve the well-being of ethnic minorities children with work-away parents and closing the nutrition gaps of the rural and urban become the key for China to further …

Rna Polymerase Binding Protein A (Rbpa) Regulation Of Mycobacteria Transcription And Sensitivity To Fidaxomicin, Jerome Prusa

Arts & Sciences Graduate Student Theses and Dissertations

Mycobacterium tuberculosis is the causative agent of the disease tuberculosis (TB) and remains one of the deadliest microorganisms on the planet. The effort to eradicate M. tuberculosis would benefit from the development of novel therapeutics, which requires a detailed understanding of M. tuberculosis physiology. Like all living organisms, M. tuberculosis gene expression requires transcription. Transcription in the phylum Actinobacteria, which includes mycobacteria, is unique because it includes RNA Polymerase Binding Protein A (RbpA) that is essential in both M. tuberculosis and the nonpathogenic model organism Mycobacterium smegmatis. RbpA increases the housekeeping A and housekeeping like B interactions with the RNA …

Exploring The Intrinsic And Extrinsic Factors That Regulate Breast Cancer Cell Dormancy, Qihao Ren

Arts & Sciences Graduate Student Theses and Dissertations

Breast cancer can recur in patients months to decades after initial diagnosis and treatment. There is mounting evidence that dormant breast disseminated tumor cells (DTCs) exist in distant organs, whose reactivation results in cancer recurrence. However, the mechanisms that control tumor cell dormancy remain poorly understood, making it difficult to predict which patients will recur and develop cancer recurrence. Unfortunately, the extreme rarity of dormant DTCs has been the major obstacle to their study. To overcome this challenge, we developed an efficient system to isolate and study rare dormant tumor cells from metastatic organs. Using this system and single cell …

Functional Optical Imaging Of The Developing Mouse Cortex In Health And Disease, Rachel Marlene Rahn

Arts & Sciences Graduate Student Theses and Dissertations

Neurodevelopmental disorders (NDDs) are a heterogeneous family of disorders characterized by the presence of abnormal developmental trajectories. Functional connectivity (FC) neuroimaging provides a minimally-invasive method by which to investigate the progression of these disorders and identify potential biomarkers of disease-related dysfunction for use in therapeutics’ development. My thesis work therefore took several approaches to the question of how the functional connectome reflects developmental change as well as system-wide perturbations from environmental or genetic factors. I explored the use of optical fluorescence and intrinsic signal imaging to characterize FC and stimulation-derived responses in a NDD model of perinatal exposure to selective …

The Origins & Functional Effects Of Postzygotic Mutations Throughout The Human Lifespan, Nicole Briana Rockweiler

Arts & Sciences Graduate Student Theses and Dissertations

Mosaicism is pervasive in humans and yet we understand little of its causes and functional consequences across the lifespan. To help solve these mysteries, we developed a suite of tools, called Lachesis, to analyze postzygotic mutations (PZMs). LachesisDetect is a novel and accurate method to detect PZMs with VAFs as low as 0.04% from bulk RNA-seq samples. LachesisMap is an innovative supervised method to reconstruct postzygotic mutation phylogenies from putative prenatal PZMs. We applied Lachesis to 17,382 samples derived from 948 donors across 54 diverse tissues and cell types from the NIH’s Genotype-Tissue Expression (GTEx) project to produce the most …

Storage Phosphor Proton Therapy Dosimetry, Jufri Setianegara

Arts & Sciences Graduate Student Theses and Dissertations

Proton beams have been increasingly utilized for radiation therapy purposes as they can confer unique radiotherapeutic advantages due to their highly targeted dose deposition behavior within a narrow Bragg peak volume. While this allows for the creation of highly conformal proton treatment plans using advanced treatment planning methods like intensity modulated proton therapy (IMPT), their inherently steep 3D spatial dose gradients make their clinical delivery challenging. As such, accurate and precise dose verification measurements are required to guarantee patient safety prior to actual treatment. Ideally, these measurements should be performed by a reusable, multi-dimensional dosimeter that is both water-equivalent and …

Utilization Patterns Of Community-Based Mental Health Services Among School-Going Adolescent Girls In Southwestern Uganda., William Byansi

Arts & Sciences Graduate Student Theses and Dissertations

ABSTRACT OF THE DISSERTATIONGlobally an estimated 20% of children and adolescents experience a disabling mental illness (Belfer, 2008). Mental health disorders are the second leading cause of disease burden and the eleventh leading cause in older adolescents (15-19 years). Yet, children and adolescents are over-represented in low-resourced countries and settings often characterized by violence, wars, diseases, physical and sexual abuse, all of which are associated with poor mental health functioning (Kieling et al., 2011; Naker, 2005). Moreover, in LMICs the treatment gap is about 93% of individuals without access to mental health services with virtually no coverage of evidence-based interventions …

Peripheral Nerve Macrophages And Their Implications In Neuroimmunity, Peter Leon Wang

Arts & Sciences Graduate Student Theses and Dissertations

Macrophages are innate immune cells that protect against pathogens and maintain tissue integrity. In vertebrates, macrophages reside in every tissue where they perform specific functions from early development through adulthood. While macrophages provide important functions across all tissues, a major focus in recent years has been the role of resident brain macrophages, known as microglia, in neurodegeneration. As microglia have been shown to affect brain development, homeostasis, and disease, they demonstrate how immune cells critically mediate neurological health and point to the broader significance of neuroimmune interactions, or the coordinated actions of the nervous and immune systems for maintaining tissue …

Variant Allele Fraction Of Genomic Alterations In Circulating Tumor Dna (%Ctdna) Correlates With Suv, Amin Haghighat Jahromi, Matthew Zabel, Ryosuke Okamura, Carl K Hoh, Razelle Kurzrock

Open Access Publications

The relationship between higher variant allele fraction (VAF) of genomic alterations in circulating tumor DNA (%ctDNA), an indicator of poor outcome, and maximum standardized uptake value (SUV

Problem-Based Learning And Thinking Style Impact On Information Literacy Skill Improvement Among Islamic Education Department Students, A Gani, Siti Zulaikhah, Kamran Asat Irsyady, Ferry Muhammadsyah Siregar

Library Philosophy and Practice (e-journal)

The information literacy skill in Islamic Education is very important for students to achieve learning goals. Therefore, this research aims to analyze the impact of the problem-based learning model’s implementation and thinking styles on information literacy skill improvement among Islamic Education Department students. This is a quasi research with data collected through test instruments as well as questionnaires, and then analyzed descriptively and inferentially. Furthermore, the data processing results of information literacy skills and thinking styles were based on the SPSS v.25 programs using two-way analysis of variance with different cells. Based on the analysis of the differences in two …

Fostering Open Access Through Online Academic Publishing: The Role Of Academic Libraries In Nigeria, Peter Olubunmi Olubiyo, Victoria Yemi Fagbemi (Mrs)

Library Philosophy and Practice (e-journal)

The paper explore the meaning of open access and online academic publishing. In teaching and learning, open access provide wide range barrier free information to teacher and learner. The paper discussed the various open access journals, online academic publishing and prominent open access publishers. It explained the roles of open access through online academic publishing and the roles of academic libraries in promoting open access. The paper recommended among others that academic library resources should be digitized to allow for open accesses and faculty should publish online in the digital age for open access.

Issues Related To Use And Acceptance Of Teachers Regarding Institutional Repositories, Susmita Das, Mukut Sarmah

Library Philosophy and Practice (e-journal)

The Institutional Repository (IR) provides access to a vast collection of digital documents created and published by academic organisations. Institutional Repository (IR) services are provided to clients by major R& D institutions and a few academic institutes in India. Top scientists and researchers may use this institutional repository (IR) technology to submit their work, making it easier for the intended audience to obtain research papers in digital form. Institutional repositories (IRs) having gotten a lot of interest from academics from many fields and all around the world. They may have improved the public's perception of academics and related institutions by …

Does Landcare Result In Changes In Perceptions Of Livestock Keepers On Sustainable Use Of Natural Resources? An Example From Sterkspruit In South Africa, W. M. Goqwana, C. Machingura, Z. Mdlulwa, R. Mkhari, O. Mmolaeng, A. O. Selomane

IGC Proceedings (1977-2023)

No abstract provided.

Developing Tropical Forage Technologies With Farmers In China, Kexian Yi, Jun Tang, Huaxuan He, Hanlin Zhou, Wangliang Xia

IGC Proceedings (1977-2023)

No abstract provided.

Farmers' Awareness, Reaction And Livlihood To The Policy Of Returning Cultivated Land And Grazing Land To Grasses In Ningxia, China, Aitao Zhao

IGC Proceedings (1977-2023)

No abstract provided.

Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat tenderness is an important quality trait critical to consumer acceptance, and determines satisfaction, repeat purchase and willingness-to-pay premium prices. Recent advances in tenderness research from a variety of perspectives are presented. Our understanding of molecular factors influencing tenderization are discussed in relation to glycolysis, calcium release, protease activation, apoptosis and heat shock proteins, the use of proteomic analysis for monitoring changes, proteomic biomarkers and oxidative/nitrosative stress. Each of these structural, metabolic and molecular determinants of meat tenderness are then discussed in greater detail in relation to animal variation, postmortem influences, and changes during cooking, with a focus on recent …

Inhibition Of Aminoglycoside 6’-N-Acetyltransferase Type Ib [Aac(6′)-Ib]: Structure-Activity Relationship Of Substituted Pyrrolidine Pentamine Derivatives As Inhibitors, Kenneth Rocha, Jesus Magallon, Craig Reeves, Kimberly Phan, Peter Vu, Crista L. Oakley-Havens, Stella Kwan, Maria S. Ramirez, Travis Lavoi, Haley Donow, Prem Chapagain, Radleigh Santos, Clemencia Pinilla, Marc A. Giulianotti, Marcelo E. Tolmasky

Mathematics Faculty Articles

The aminoglycoside 6′-N-acetyltransferase type Ib [AAC(6′)-Ib] is a common cause of resistance to amikacin and other aminoglycosides in Gram-negatives. Utilization of mixture-based combinatorial libraries and application of the positional scanning strategy identified an inhibitor of AAC(6′)-Ib. This inhibitor’s chemical structure consists of a pyrrolidine pentamine scaffold substituted at four locations (R1, R3, R4, and R5). The substituents are two S-phenyl (R1 and R4), an S-hydroxymethyl (R3), and a 3-phenylbutyl (R5) groups. Another location, R2, does not have a substitution, but it is named because its stereochemistry was modified in some compounds utilized in this study. Structure-activity …

Space Opens Its Doors To Unexpected Players, One Of Them Paraguay, Macarena Carrizosa

Capstones

Paraguay successfully sent its first native satellite into orbit in 2021, proving how essential and accessible the industry has become, even for developing countries. This article looks at how knowledge and technology transfer programs combined with a lower cost of entry helped Paraguay reach its first major milestone in the space industry, having its own satellite.

Proceedings Of The 2021 Berry Summer Thesis Institute, University Of Dayton. University Honors Program

Proceedings of the Berry Summer Thesis Institute (2013-Present)

Thanks to a gift from the Berry Family Foundation and the Berry family, the University Honors Program launched the Berry Summer Thesis Institute in 2012. The institute introduces students in the University Honors Program to intensive research, scholarship opportunities and professional development. Each student pursues a 12-week summer thesis research project under the guidance of a UD faculty mentor. This contains the product of the students' research.

Comparative Analysis Of Longevity And Stress Resiliency Benefits Of Supplementation With High-Antioxidative Capacity Juices In Organisms That Are Representative Of Humans, Meghan Long

Honors Scholar Theses

Neurodegenerative disease presents itself in a complex array of illnesses that is increasingly affecting the geriatric population. There has not been research on a common cause since there are a culmination of reasons that are probable ranging from genetic to environmental factors. Antioxidants have been known to prevent and protect against the damage that is otherwise caused by reactive oxygen species (ROS). Thus, high-capacity antioxidant juices supplemented into the diet of animal models such as Caenorhabditis elegans, Drosophila melanogaster, rats, and humans will allow for the study of the possible benefits.

Although a conclusion and common causation on the usage …

In Vitro Antioxidant And Antihypertensive Activity Of Edible Insects Flours (Mealworm And Grasshopper) Fermented With Lactococcus Lactis Strains, Adilene Mendoza-Salazar, Lourdes Santiago-Lopez, Maria J. Torres-Llanez, Adrian Hernandez-Mendoza, Belinda Vallejo-Cordoba, Andrea M. Liceaga, Aaron E. Gonzalez-Cordova

Department of Food Science Faculty Publications

The objective of the present study was to evaluate the potential antioxidant and angiotensin converting enzyme inhibition (ACEI) activity of edible insect flours fermented with Lactococcus lactis strains. For the fermentation, mealworm and grasshoppers flours were dissolved (0.5% w/v) in buffer solution (pH 7.0) and individually inoculated (3%) with Lactococcus lactis strains (NRRL B-50571, NRRL B-50572). The samples were incubated for 72 h at 30 ◦C, and the pH was recorded. The degree of hydrolysis (DH) and protein content were determined. The total polyphenol compounds, antioxidant activity (ABTS, DPPH, ORAC, and FRAP), and ACEI of the <3 kDa fractions were ana- lyzed. The pH of the fermented samples decreased to 3.5–3.9 (p < 0.05). The fermented grasshopper flour showed an increased DH (0.42%) and overall higher total polyphenol content (8.23 mg Gallic Acid Equivalent/mL). In general, the highest antioxidant activity was for the grasshopper fractions fermented for 24 h by Lactococcus lactis NRRL B-50572, which also showed 23.47% ACEI inhibition with an IC50 of 0.97 mg/mL. The peptide profile obtained increased after fermentation, being higher for the mealworm flour fermented sample. This study presents, for the first time, the use of specific strains of Lactococus lactis for fermenting edible insect-derived products in the production of bioactive compounds with potential antioxidant and antihypertensive activity.

Sars-Cov-2 Tests: Bridging The Gap Between Laboratory Sensors And Clinical Applications, Nikita Toropov, Eleanor Osborne, Lovleen Tina Joshi, James Davidson, Caitlin Morgan, Joseph Page, Justin Pepperell, Frank Vollmer

Peninsula Dental School

No abstract provided.

The Role Of Thyroid Stimulating Hormone In The Timing Of Thyroxin Supplement After Thyroid Operations, Basil Abdulrazzaq Saadallah Al-Khayyat, Berq J. Hadi Al-Yasseri

Muthanna Medical Journal

Thyroid Stimulating Hormone (TSH) levels can be measured accurately down to a very low serum concentration with an immunoassay. When the serum TSH level is in the normal range, measuring the T3 and T4 levels is redundant. The objective of this study is to study the relation of TSH levels postoperatively in thyroid surgeries with the timing for thyroxin treatment as a supplemental and suppressive therapy. A prospective cohort study was done on 84 patients underwent thyroid operations in Al-Yarmouk Teaching Hospital from March 2010 through November 2012. Patients underwent different thyroid operations (lobectomy, subtotal thyroidectomy and total thyroidectomy) for …

Citation Analysis Of Bldea’S Professional Institutes, Vijayapura: A Scientometric Study, Dr. Bachalapur M.M., Dr. Prasanna Kumara Bm, Dr. Jayaprakash G. Hugar

Library Philosophy and Practice (e-journal)

This paper aims to evaluate the research productivity and citations received by the BLDEA’s professional institutions established in Vijayapura, Karnataka State, India under the banner of BLDE Association's based on their publications and citations as reflected in the Scopus Citation Database for the period of ten years from 2011 to 2020. A total of 910 research publications and citations with all metadata have been downloaded and analyzed as per the objectives of the current study. These three professional institutions received four thousand two hundred forty-seven citations. The paper tries to provide all measurements to view the research output and citations …

A Timeline Of Discovery And Current Research On Primary Open-Angle Glaucoma And Emergence Of Potentially Permanent Treatment Solutions, Yonosuke Dejesus, Guadalupe Moreno Ceballos

Spectra Undergraduate Research Journal

This research examines the timeline of the discovery and research of Primary Open-Angle Glaucoma (POAG). By reviewing the literature on genetic and molecular mechanisms, we aim to emphasize a long-term treatment solution (iSTENT) to mitigate intraocular pressure (IOP) related to POAG etiology and disease progression. POAG is a multifactorial, autosomal dominant, adult-onset eye disease wherein the optic nerve cells become damaged due to a buildup of excess aqueous humor, resulting in increased IOP. Consequently, POAG leads to progressive loss of peripheral vision and is the leading cause of blindness in the US. Due to the multifactorial nature of glaucoma’s inheritance …

Grass‐Planting And Sheep‐Raising Skills Development In Rocky Desertification Mountain Area, Jiahai Wu, Qiong Mou, Chengbing Tang, Xiangyong Zhao, Daquan Zhang, Guonian Xie

IGC Proceedings (1977-2023)

No abstract provided.

Master Grazer: Educating Kentucky Livestock Producers, S. Ray Smith, Garry D. Lacefield

IGC Proceedings (1977-2023)

No abstract provided.