Digital Commons Network^™

Dna-Encoded Chemistry Technology Yields Expedient Access To Sars-Cov-2m Pro Inhibitors, Srinivas Chamakuri, Shuo Lu, Melek Nihan Ucisik, Kurt M Bohren, Ying-Chu Chen, Huang-Chi Du, John C Faver, Ravikumar Jimmidi, Feng Li, Jian-Yuan Li, Pranavanand Nyshadham, Stephen S Palmer, Jeroen Pollet, Xuan Qin, Shannon E Ronca, Banumathi Sankaran, Kiran L Sharma, Zhi Tan, Leroy Versteeg, Zhifeng Yu, Martin M Matzuk, Timothy Palzkill, Damian W Young

Faculty, Staff and Students Publications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has killed more than 4 million humans globally, but there is no bona fide Food and Drug Administration–approved drug-like molecule to impede the COVID-19 pandemic. The sluggish pace of traditional therapeutic discovery is poorly suited to producing targeted treatments against rapidly evolving viruses. Here, we used an affinity-based screen of 4 billion DNA-encoded molecules en masse to identify a potent class of virus-specific inhibitors of the SARS-CoV-2 main protease (Mpro) without extensive and time-consuming medicinal chemistry. CDD-1714, the initial three-building-block screening hit (molecular weight [MW] = 542.5 g/mol), was a potent inhibitor (inhibition …

Dna-Encoded Chemistry Technology Yields Expedient Access To Sars-Cov-2m Pro Inhibitors, Srinivas Chamakuri, Shuo Lu, Melek Nihan Ucisik, Kurt M Bohren, Ying-Chu Chen, Huang-Chi Du, John C Faver, Ravikumar Jimmidi, Feng Li, Jian-Yuan Li, Pranavanand Nyshadham, Stephen S Palmer, Jeroen Pollet, Xuan Qin, Shannon E Ronca, Banumathi Sankaran, Kiran L Sharma, Zhi Tan, Leroy Versteeg, Zhifeng Yu, Martin M Matzuk, Timothy Palzkill, Damian W Young

Faculty, Staff and Students Publications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has killed more than 4 million humans globally, but there is no bona fide Food and Drug Administration–approved drug-like molecule to impede the COVID-19 pandemic. The sluggish pace of traditional therapeutic discovery is poorly suited to producing targeted treatments against rapidly evolving viruses. Here, we used an affinity-based screen of 4 billion DNA-encoded molecules en masse to identify a potent class of virus-specific inhibitors of the SARS-CoV-2 main protease (Mpro) without extensive and time-consuming medicinal chemistry. CDD-1714, the initial three-building-block screening hit (molecular weight [MW] = 542.5 g/mol), was a potent inhibitor (inhibition …

Four New Lithophilic Species Of Scorpiops Peters, 1861 (Scorpiones: Scorpiopidae) From Peninsular India, Shauri Sulakhe, Shubhankar Deshpande, Nikhil Dandekar, Anand Padhye, Deshabhushan Bastawade

Euscorpius

Four new lithophilic species of Scorpiops Peters, 1861 (Scorpiopidae) have been described from peninsular India using an integrated taxonomic approach. Three species Scorpiops vrushchik sp. n., S. nagphani sp. n. and S. neera sp. n. have been described from northern Western Ghats and S. lioneli sp. n. is described from Deccan Plateau. S. vrushchik sp. n. is closely related to S. tenuicauda and S. telbaila and differs by a raw genetic divergence of 9.6-9.8% from S. tenuicauda and 11% from S. telbaila. S. nagphani sp. n. is closely related to S. lioneli sp. n. and S. maharashtraensis and differs by …

The Onset Of Exercise-Associated Hyponatremia And Individual Differences In Inappropriate Arginine Vasopressin Excretion: A Review Of Proposed Mechanisms, Michelle Stehman, Stephen A. Maris

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 2: Issue 1, Article 10, 2021. Exercise-associated hyponatremia (EAH) has been reported to develop during endurance events such as triathlons and marathons. As these events become more popular, the incidence of developing EAH also increases. The development of EAH is commonly associated with the overconsumption of hypotonic fluids such as water and tends to be more prevalent in females. There is also evidence to suggest the inappropriate secretion of arginine vasopressin (AVP) leading to water retention may predispose an individual for developing EAH, especially when coupled with the overconsumption of fluids. Recent research …

Science Mapping And Visualization Of Research Data Management (Rdm): Bibliometric And Scientometric Study, Nidhi Gupta Research Scholar, Surbhi Arora, Prof. Rupak Chakravarty

Library Philosophy and Practice (e-journal)

Research Data Management (RDM) is an ever-evolving phenomenon focused to augment and manage the research capital of an organization, especially in context with developing countries. This study aims at to understand and highlight the state of art of RDM literature between 1989 and 2021 using Web of Science Core Collection (WoSCC) database and VOSviewer software with a bibliometric or scientometric approach thereby highlighting the most influential authors, countries, journals, institutions, and to their co-authorship pattern, co- citation pattern, bibliographic coupling pattern, the co-occurrence of keywords pattern in the field of RDM.The data comprises of a total of 797 documents ,further …

Selection Of High Yielding And Anthracnose Resistant Stylosanthes For Brazil, India And China, N. White, S. Chakraborty, Celso D. Fernandes, M. J. Charchar, N. P. Melkania, C. R. Ramesh, B. Gangaiah, Guodao Liu, D. F. Cameron, J. W. Miles

IGC Proceedings (1977-2023)

Resistance to anthracnose, dry matter yield (DMY) and seed yield (SY) was assessed for germplasm and breeding lines of Stylosanthes in Brazil, India and China. Overall, Stylosanthes guianensis produced higher DMY than S. scabra, S. capitata and S. macrocephala at most sites in Brazil. Data from China suggest that there are high yielding anthracnose resistant S. guianensis lines that can reduce the reliance on CIAT 184. S. seabrana might also prove successful. S. seabrana in India produced the highest DMY and SY and it can form nodules with native Bradyrhizobium strains. Regional differences in resistance within accessions stress the …

Developmental Programming Of Dna Methylation And Gene Expression Patterns Is Associated With Extreme Cardiovascular Tolerance To Anoxia In The Common Snapping Turtle, Ilan Ruhr, Jacob Bierstedt, Turk Rhen, Debojyoti Das, Sunil Kumar Singh, Soleille Miller, Dane A. Crossley Ii, Gina L. J. Galli

Biology Faculty Publications

Background

Environmental fluctuation during embryonic and fetal development can permanently alter an organism’s morphology, physiology, and behaviour. This phenomenon, known as developmental plasticity, is particularly relevant to reptiles that develop in subterranean nests with variable oxygen tensions. Previous work has shown hypoxia permanently alters the cardiovascular system of snapping turtles and may improve cardiac anoxia tolerance later in life. The mechanisms driving this process are unknown but may involve epigenetic regulation of gene expression via DNA methylation. To test this hypothesis, we assessed in situ cardiac performance during 2 h of acute anoxia in juvenile turtles previously exposed to normoxia …

Pragmatism And Faith: A University Administrator’S Reading Of Reinhold Niebuhr, Jon Young

Government and History Faculty Working Papers

This paper examines university administration and theology based on the author’s growing appreciation for the writings of Christian theologian, Reinhold Niebuhr (1892-1971), while serving in the senior administrative roles of provost and chief of staff at Fayetteville State University in North Carolina. One of the leading Protestant theologians of the twentieth century, Niebuhr is perhaps best known as the writer of the “Serenity Prayer,” but his analyses of political and social events of the early and midtwentieth century influenced Martin Luther King, Jr., Jimmy Carter, and Barack Obama, among many others. Niebuhr’s writings enjoyed a resurgence of interest in the …

The Interaction And Progression Of Adolescent Substance Use And Co-Occurring Mental Health Problems And Social Support: A Latent Growth Modeling Approach, Jeremiah D. Simmons

Psychology ETDs

Background: Few studies have examined a reciprocal parallel process longitudinal trajectory of adolescent substance use and mental health problems simultaneously, and none have described this parallel process for adolescents receiving outpatient substance use treatment. No studies have examined how social support may account for deviations outside the overall trajectories for substance use or mental health problems. Method: Secondary data analysis examined a longitudinal parallel process latent growth model between mental health and substance use problems among adolescents who received outpatient substance use treatment. Results: Findings suggest that mental health and substance use problems are associated both initially and longitudinally, and …

Editorial: Roles Of Sleep Disruption And Circadian Rhythm Alterations On Neurodegeneration And Alzheimer's Disease, Marilyn J. Duncan, Sigrid C. Veasey, Phyllis Zee

Neuroscience Faculty Publications

No abstract provided.

Relationship Between Food Insecurity And Smoking Status Among Women Living With And At Risk For Hiv In The Usa: A Cohort Study, Lila A. Sheira, Edward A. Frongillo Jr., Judith Hahn, Kartika Palar, Elise D. Riley, Tracey E. Wilson, Adebola Adedimeji, Daniel Merenstein, Mardge Cohen, Eryka L. Wentz, Adaora A. Adimora, Ighovwerha Ofotokun, Lisa Metsch, Janet M. Turan, Phyllis C. Tien, Sheri D. Weiser

Faculty Publications

Objectives People living with HIV (PLHIV) in the USA, particularly women, have a higher prevalence of food insecurity than the general population. Cigarette smoking among PLHIV is common (42%), and PLHIV are 6–13 times more likely to die from lung cancer than AIDS related causes. This study sought to investigate the associations between food security status and smoking status and severity among a cohort of predominantly low-income women of colour living with and without HIV in the USA.

Design Women enrolled in an ongoing longitudinal cohort study from 2013 to 2015.

Setting Nine participating sites across the USA.

Participants 2553 …

Vol. 61, No. 03 (September 6, 2021)

Indiana Law Annotated

No abstract provided.

Crow's Nest, September 6, 2021, Crow's Nest Staff

Crow's Nest

The September 6, 2021, issue of the Crow's Nest.

Humes High School Herald, Memphis, 1925

Shelby County, Tennessee

Vol. 1, No. 1 of the "Humes High Herald", published by the students of Humes High School in Memphis, Tennessee, on April 8, 1925. L.C. Humes High School, at 659 N. Manassas Street, was open as a high school from the 1920s through 1967.

Apoε4 Lowers Energy Expenditure In Females And Impairs Glucose Oxidation By Increasing Flux Through Aerobic Glycolysis, Brandon C. Farmer, Holden C. Williams, Nicholas A. Devanney, Margaret A. Piron, Grant K. Nation, David J. Carter, Adeline E. Walsh, Rebika Khanal, Lyndsay E. A. Young, Jude C. Kluemper, Gabriela Hernandez, Elizabeth J. Allenger, Rachel Mooney, Lesley R. Golden, Cathryn T. Smith, J. Anthony Brandon, Vedant A. Gupta, Philip A. Kern, Matthew S. Gentry, Josh M. Morganti, Ramon C. Sun, Lance A. Johnson

Physiology Faculty Publications

BACKGROUND: Cerebral glucose hypometabolism is consistently observed in individuals with Alzheimer's disease (AD), as well as in young cognitively normal carriers of the Ε4 allele of Apolipoprotein E (APOE), the strongest genetic predictor of late-onset AD. While this clinical feature has been described for over two decades, the mechanism underlying these changes in cerebral glucose metabolism remains a critical knowledge gap in the field.

METHODS: Here, we undertook a multi-omic approach by combining single-cell RNA sequencing (scRNAseq) and stable isotope resolved metabolomics (SIRM) to define a metabolic rewiring across astrocytes, brain tissue, mice, and human subjects expressing APOE4.

RESULTS: Single-cell …

Systemic Copper Disorders Influence The Olfactory Function In Adult Rats: Roles Of Altered Adult Neurogenesis And Neurochemical Imbalance, Sherleen Xue-Fu Adamson, Wei Zheng, Zeynep Sena Agim, Sarah Du, Sheila Fleming, Jason Cannon

School of Health Sciences Faculty Publications

Disrupted systemic copper (Cu) homeostasis underlies neurodegenerative diseases with early symptoms including olfactory dysfunction. This study investigated the impact of Cu dyshomeostasis on olfactory function, adult neurogenesis, and neurochemical balance. Models of Cu deficiency (CuD) and Cu overload (CuO) were established by feeding adult rats with Cu-restricted diets plus ip. injection of a Cu chelator (ammonium tetrathiomolybdate) and excess Cu, respectively. CuD reduced Cu levels in the olfactory bulb (OB), subventricular zone (SVZ), rostral migratory stream (RMS), and striatum, while CuO increased Cu levels in these areas. The buried pellet test revealed both CuD and CuO prolonged the latency to …

The Concurrence Of Dna Methylation And Demethylation Is Associated With Transcription Regulation, Jiejun Shi, Jianfeng Xu, Yiling Elaine Chen, Jason Sheng Li, Ya Cui, Lanlan Shen, Jingyi Jessica Li, Wei Li

Children’s Nutrition Research Center Staff Publications

The mammalian DNA methylome is formed by two antagonizing processes, methylation by DNA methyltransferases (DNMT) and demethylation by ten-eleven translocation (TET) dioxygenases. Although the dynamics of either methylation or demethylation have been intensively studied in the past decade, the direct effects of their interaction on gene expression remain elusive. Here, we quantify the concurrence of DNA methylation and demethylation by the percentage of unmethylated CpGs within a partially methylated read from bisulfite sequencing. After verifying 'methylation concurrence' by its strong association with the co-localization of DNMT and TET enzymes, we observe that methylation concurrence is strongly correlated with gene expression. …

A Novel Multimodal Biometric Authentication System Using Machine Learning And Blockchain, R Brown, G Bendiab, S Shiaeles, B Ghita

School of Engineering, Computing and Mathematics

No abstract provided.

Editorial: Vitamin D Deficiency And Sufficiency In Reproduction And Bone Metabolism, Rehana Rehman, Faiza Alam, Mukhtiar Baig, Aysha Habib Khan, Naseer Ahmed

Department of Biological & Biomedical Sciences

No abstract provided.

Whole Genome Sequence Analysis Of Platelet Traits In The Nhlbi Trans-Omics For Precision Medicine Initiative, Amarise Little, Yao Hu, Quan Sun, Deepti Jain, Jai G. Broome, Ming-Huei Chen, Florian Thibord, Caitlin Mchugh, John Blangero, Joanne E. Curran

School of Medicine Publications

Platelets play a key role in thrombosis and hemostasis. Platelet count (PLT) and mean platelet volume (MPV) are highly heritable quantitative traits, with hundreds of genetic signals previously identified, mostly in European ancestry populations. We here utilize whole genome sequencing from NHLBI's Trans-Omics for Precision Medicine Initiative (TOPMed) in a large multi-ethnic sample to further explore common and rare variation contributing to PLT (n = 61 200) and MPV (n = 23 485). We identified and replicated secondary signals at MPL (rs532784633) and PECAM1 (rs73345162), both more common in African ancestry populations. We also observed rare variation in Mendelian platelet …

Being The Father Of A Preterm-Born Child: Contemporary Research And Recommendations For Nicu Staff, Franco Baldoni, Gina Ancora, Jos M. Latour

School of Nursing and Midwifery

No abstract provided.

Quality Of Life Analyses In Patients With Multiple Myeloma: Results From The Selinexor (Kpt-330) Treatment Of Refractory Myeloma (Storm) Phase 2b Study, Gabriel Tremblay, Ravi Vij, Et Al.

2020-Current year OA Pubs

BACKGROUND: Selinexor is an oral, selective nuclear export inhibitor. STORM was a phase 2b, single-arm, open-label, multicenter trial of selinexor with low dose dexamethasone in patients with penta-exposed relapsed/refractory multiple myeloma (RRMM) that met its primary endpoint, with overall response of 26% (95% confidence interval [CI], 19 to 35%). Health-related quality of life (HRQoL) was a secondary endpoint measured using the Functional Assessment of Cancer Therapy - Multiple Myeloma (FACT-MM). This study examines impact of selinexor treatment on HRQoL of patients treated in STORM and reports two approaches to calculate minimal clinically important differences for the FACT-MM.

METHODS: FACT-MM data …

Delineating Conservation Areas For Cohabiting Species: An Example Of Vernal Pond Conservation From Fort Stewart In Georgia, United States, Yicheng Wang, Hayri Önal, Sahan T. M. Dissanayake

Economics Faculty Publications and Presentations

Military installations are valuable in global biodiversity conservation as they secure representative ecosystems from land conversion and protect many threatened or endangered species. Selecting suitable areas for biodiversity conservation within military installations is a challenging problem as this must not impede military training activities. The issue gets more complicated when considering multiple cohabiting species in a metacommunity with species dependency. In this paper, we present an example for the conservation of two cohabiting species, Gopher Tortoise (GT) and Gopher Frog (GF), located within the boundaries of a military installation, Fort Stewart, Georgia, United States. The GF depends on both locations …

Adar And Hnrnpc Deficiency Synergize In Activating Endogenous Dsrna-Induced Type I Ifn Responses, Anna-Maria Herzner, Zia Khan, Eric L Van Nostrand, Sara Chan, Trinna Cuellar, Ronald Chen, Ximo Pechuan-Jorge, Laszlo Komuves, Margaret Solon, Zora Modrusan, Benjamin Haley, Gene W Yeo, Timothy W Behrens, Matthew L Albert

Faculty, Staff and Students Publications

Cytosolic double-stranded RNA (dsRNA) initiates type I IFN responses. Endogenous retroelements, notably Alu elements, constitute a source of dsRNA. Adenosine-to-inosine (A-to-I) editing by ADAR induces mismatches in dsRNA and prevents recognition by MDA5 and autoinflammation. To identify additional endogenous dsRNA checkpoints, we conducted a candidate screen in THP-1 monocytes and found that hnRNPC and ADAR deficiency resulted in synergistic induction of MDA5-dependent IFN responses. RNA-seq analysis demonstrated dysregulation of Alu-containing introns in hnRNPC-deficient cells via utilization of unmasked cryptic splice sites, including introns containing ADAR-dependent A-to-I editing clusters. These putative MDA5 ligands showed reduced editing in the absence of ADAR, …

Genetic Diversity In The Anthracnose Pathogen Infecting Stylosanthes In Brazil, India And China, P. Weeds, S. Chakraborty, Celso D. Fernandes, M. J. Charchar, C. R. Ramesh, Guodao Liu, S. Kelemu

IGC Proceedings (1977-2023)

This work aimed to determine the genetic diversity of Colletotrichum gloeosporioides infecting Stylosanthes spp. in Brazil, China and India. A total of 132 isolate originating from S. seabrana, S. macrocephala, S. capitata, S. scabra, and S. guianensis were used. Four major genetic groups were identified from an analysis of genetic diversity using selection-neutral DNA markers. Group 1 contained 20 isolates and this may represent a genotype that migrated from the center of diversity in Brazil and Colombia to Australia, Thailand and India. Group 2 consisted of 66 Brazilian isolates and group 3 had 19 isolates from …

Relationship Between Tree Density And Grass Dry Matter Yield In A Southern African Savanna, G. N. Smit

IGC Proceedings (1977-2023)

The investigation was carried out on a densely wooded area in the Mopani savanna of South Africa. Seven plots were subjected to different intensities of tree thinning, ranging from a totally cleared plot (0 %) to plots thinned to the equivalent of 10 %, 20 %, 35 %, 50 % and 75 % of the leaf biomass of a control plot (100 %). The grasses responded positively to the tree thinning in terms of total dry matter yield. The yields between tree canopies were generally of the same order or higher than under tree canopies, with the yields where trees …

Seasonal Patterns Of Growth And Senescence In Cynodon Spp. Cv Tifton 85 Grazed Swards, L. F. M. Pinto, L. G. Barioni, S. C. Da Silva

IGC Proceedings (1977-2023)

Growth and senescence are very important determinants of grassland productivity and correspond to key features to be considered for grazing management purposes. The present study was carried out at ESALQ, Piracicaba, S.P., Brazil and evaluated growth and senescence as a function of sward surface height. Mathematical models generated revealed that a similar pattern of response occurred as described in the literature for temperate grass species. However, a seasonal variation in response curves for growth and senescence was also observed and could be related to climatic events. Highest herbage accumulation rates were observed within the range of 15 to 20 cm …

Yields And Sward Characteristics Of Timothy Cultivars Under Grazing, H. T. Kunelius, G. H. Dürr, K. B. Mcrae, S. A. E. Fillmore

IGC Proceedings (1977-2023)

Seven timothy (Phleum pratense L.) cultivars were evaluated over three pasture seasons under rotational grazing to 7 or 11 cm post-grazing heights and regrowth periods of four to seven weeks. There was a trend for the pasture type timothy cultivars to outyield Champ timothy (check). Dry matter yields were lower for the 7 cm than 11 cm post-grazing height. Although the overall cultivar x post-grazing height interaction was not significant (P > 0.05), there was variability in grazing tolerance among timothy cultivars. Yield of Kahu was maintained and vegetative tiller density of Kahu increased over the experimental period under 7 …

A Technique For Assessing Seed Survival Of New Pasture Legumes Following Grazing By Sheep, Caroline T. De Koning, P. E. Beale, A. J. Duncan, S. Hughes, K. Haskard

IGC Proceedings (1977-2023)

A technique for assessing the fate of seed grazed by sheep in small field plots was examined using the pasture species Trifolium michelianum cv. Paradana, Medicago truncatula cv. Mogul, Trifolium spumosum and Trigonella balansae. Trifolium spumosum lost the greatest proportion of seed from dry pasture residues (88% seed eaten), whereas Trigonella balansae, Paradana and Mogul lost 61%, 55% and 53% respectively. However, Trifolium spumosum and Paradana had high levels of seed in the faeces (56.8 kg/ha and 65.2 kg/ha respectively), whereas Mogul and Trigonella balansae had low levels (10.3 kg/ha and 11.0 kg/ha respectively). Mogul, due to it's …

Prevalence And Population At Risk For Noise Induced Hearing Loss (Nihl) In Adolescent Students, Yusni Yusni, Ikbal Ikbal, Firdalena Meutia

Folia Medica Indonesiana

Noise-Induced Hearing Loss (NIHL) is a global health problem, often underestimated, and leads to permanent hearing loss. The prevalence of NIHL in adolescents has increased in line with the increasing use of cell phones. This study aimed to determine the prevalence of NIHL and analyze the population at risk (age, gender, and school) in adolescent students. The study design was a cross-sectional study. A total of 510 students consisting of female, n=288 and male, n=222; junior high school, n=203 and senior high school, n=307 were used as research subjects. This study was conducted on August-October 2015 at Darul Ihsan School, …