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Carnitine Concentrations In Healthy And Septicaemia Suspected Neonatal Calves And Itsrelation To Passive Immunty, Erhan Gökçe, Mahmut Sözmen, Canan Gülmez, Kadi̇r Bozukluhan, Gürbüz Gökce, Emi̇ne Ataki̇şi̇, Mehmet Çi̇ti̇l, Hi̇dayet Meti̇n Erdoğan

Turkish Journal of Veterinary & Animal Sciences

This study was designed to determine carnitine concentrations in newborn calves with suspected septicaemia, healthy calves, and colostrum samples as well as to detect alterations in blood carnitine concentrations after colostrum intake. Carnitine concentrations were detected in both neonatal calves with suspected septicaemia (n = 195) and healthy calves (n =10) as well as in colostrum/milk samples from their dams (n = 20). The mean carnitine concentration on the 2nd day after colostrum intake (32.61 μmol/L) was significantly higher than that measured before colostrum intake (17.61 μmol/L) (P < 0.05). The striking result was significantly lower mean carnitine concentration detected in calves with suspected septicaemia (17.11 μmol/L), compared to healthy calves (24.92 μmol/L) (P < 0.001). Apart from a sudden increase on day 7, no significant alteration was observed in colostrum/milk carnitine concentrations throughout the postpartum period. The results indicated that colostrum contained carnitine, which passed into the bloodstream of the calf by passive colostral transfer, and that a dramatic reduction in blood carnitine concentrations of calves with suspected septicaemia existed. The results might be of help in dealing with septicaemia suspected neonatal calves through both measurement of blood carnitine and, in response, to supplement such cases with carnitine.

The Morphological And Morphometric Characteristics Of Alabadem Pigeons, Evren Erdem, Fatma Tüli̇n Özbaşer, Eser Kemal Gürcan, Mehmet İhsan Soysal

Turkish Journal of Veterinary & Animal Sciences

This study aimed to determine the morphological and morphometric characteristics of Alabadem pigeons, which are grown as ornamental pigeons in Edirne province, and to determine mutual traits with other indigenous pigeon breeds in Turkey by comparing with previous studies. Body weight (P < 0.001), head length (P < 0.01), head width (P < 0.05), beak length (P < 0.001), and tarsus diameter (P < 0.05) of age group III were higher than those of other age groups; however, the wingspan of age group II was greater than that of other age groups in Alabadem pigeon. Compared to other indigenous genotypes in Turkey, the Alabadem pigeon is a small-sized genotype. Alabadem pigeons were similar to Edremit butterfly pigeons and Thracian tumbler pigeons in terms of crested and blackeyed pigeons. On the other hand, the irregularly shaped mark (almond) on the head in Alabadem pigeons was similar to the wide-long irregular shaped mark on the neck of Edremit butterfly pigeons. Some basic plumage colors (black, yellow, and red) and intermediate colors (chickpea and scarlet) were identical to Alabadem pigeons and Thrace roller pigeons. Since Alabadem pigeons have many mutual characteristics with Edremit butterfly pigeons and Thracian roller pigeons, genetic studies may be recommended to determine the degree of relationship among these breeds.

Effects Of Honeybee (Apis Mellifera) Venom On Redox Balance, Biochemical Andhematological Profile In Diabetic Rats: A Preliminary Study, Bariş Denk, Abdurrahman Fati̇h Fi̇dan

Turkish Journal of Veterinary & Animal Sciences

The treatment of diseases with honeybee venom (apitoxin) is a branch of apitherapy, which has yet to find substantial scientific support. In this present study, we used apitoxin to investigate its effects on diabetes mellitus (DM) in rats. The use of apitoxin injection on single-dose streptozotocin (STZ)-treated rat model was selected, and the study took 28 days. Fasting blood glucose (FBG) was measured weekly. Biochemical and hematological parameters, oxidative stress markers, and insulin levels were measured from the blood samples provided on the last day of the study, and the DNA damage marker (8-OHdG) was measured from urine samples. We …

Effects Of Milk Replacer And Whole Milk Feeding On Rumen Development, Expression Ofgenes Related To Volatile Fatty Acid Absorption, And Rumen Bacteria In Calves, Ahmet Ceylan, Ali̇ Çalik, Burcu Eki̇m, Alev Gürol Bayraktaroğlu, Oğuz Çalişici, Doğukan Özen, Pinar Saçakli

Turkish Journal of Veterinary & Animal Sciences

This research examined the effects of liquid feeds on calf performance, rumen development, rumen cellulolytic bacteria count, and total bacteria diversity. Twenty Holstein bull calves were allocated to 2 groups either fed with milk replacer (MR) or whole milk. Calves fed milk tended to have higher (P = 0.058) average daily weight gain as compared to calves fed with MR during the entire experimental period. Starter mixture intake was not influenced by either milk or milk replacer feeding. Calves fed with milk had higher (P < 0.001) proliferative cells in the rumen papilla. The expressions of MCT1, MCT4, NHE1, NHE3, ACSS1, AceCS2, and ACSM1 genes, which are related to volatile fatty acid absorption, in the dorsal sac of the rumen were similar for calves fed either MR or whole milk. Fibrobacter succinogenes (P = 0.017) and Ruminococcus flavefaciens (P = 0.037) were significantly higher in the rumen of the milkfed calves. Moreover, denaturing gradient gel electrophoresis (DGGE)-derived dendrograms showed noticeable differences in ruminal microbial population between MR-fed and milk-fed calves. In conclusion, feeding calves with whole milk, which contains better quality protein and nutrients, instead of milk replacers, positively affects rumen development, cellulolytic bacteria numbers and total bacteria diversity.

Quality, Nutritional, And Sensory Attributes Of Naked Neck Chicken Eggs As Influenced Byproduction Systems And Dietary Regimens, Erum Bughio, Jibran Hussain, Athar Mahmud, Anjum Khalique, Ahmed Sultan Jatoi, Shahid Mehmood, Muhammad Usman, Sohail Ahmad

Turkish Journal of Veterinary & Animal Sciences

This study evaluated the quality, nutritional, and sensory attributes of Naked Neck chicken eggs when birds were subjected to different housing and nutritional regimens. Birds were maintained in two rearing systems (intensive & free-range) with five dietary treatments based on commercial feed (CF) and kitchen waste (KW), i.e. 100% CF, 75% CF + 25% KW, 50% CF + 50% KW, 25% CF + 75% KW, and 100% KW. A total of 500 eggs, comprised of ten per replicate (50 per treatment) were used. Higher (p ≤ 0.05) shell weight and thickness, shape index, egg volume, yolk index and weight, egg …

Associations Of Snps In Ghr Gene With Growth And Milk Yield Of Anatolian Buffaloes, Meti̇n Erdoğan, Mustafa Tekerli̇, Koray Çeli̇keloğlu, Özlem Hacan, Serdar Koçak, Zehra Bozkurt, Samet Çi̇nkaya, Mustafa Demi̇rtaş

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to investigate the associations between single nucleotide polymorphisms (SNP) in the growth hormone receptor (GHR) gene and growth and milk yields of Anatolian buffaloes. Growth records of genotyped 3012 Anatolian buffaloes and 467 lactations of 329 cows from them were used. The overall means of weights at birth, six and 12 months of ages and lactation milk yield were 29.377 ± 0.342 kg, 96.15 ± 1.48 kg, 165.54 ± 2.18 kg, and 976.3 ± 39.9 kg, respectively. Three mutations (g.31601787 G > A, g.31601784 G > A, and g.31601783 G > del) in GHR were detected. A least-squares …

Ruminal Kinetics Of Carbohydrates, Gas Production And Bromatology Of Sugarcane Silagewith High Levels Of Sodium Benzoate, Celso Heinzen Junior, Odimári Pricila Prado Calixto, Egon Henrique Horst, Leonardo Trolegi, Fernando Luiz Massaro Junior, Ivone Yurika Mizubuti, Mikael Neumann, Valter Harry Bumbieris Junior

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the chemical composition, ruminal kinetics, gas production and aerobic stability of sugarcane silage added with increasing levels of sodium benzoate up to 1% of the natural matter. After harvesting, the sugarcane was chopped and placed in experimental polyethylene silos for storage for 30 days. An increasing concentration of sodium benzoate in the silage promoted a linear decrease in the crude protein content, varying from 2.79% to 2.51%. The addition of sodium benzoate reduced the fiber concentration of the silage (P = 0.0015) and increased gas production of the fibrous and nonfibrous carbohydrates …

A New Molecular Approach To The Diagnosis Of Small Ruminant Morbillivirus Withevagreen Based Assay, Mustafa Emi̇n Öz, Emrah Torlak, Oğuzhan Avci, Müge Doğan, Ali̇ Tevfi̇k Uncu

Turkish Journal of Veterinary & Animal Sciences

The present work describes a new method that is anticipated to contribute to the global control and eradication strategies of small ruminant morbillivirus (SRMV), which negatively affects sheep and goat breeding in many countries where outbreaks emerge. Described assay was developed to detect SRMV by targeting the fusion glycoprotein gene. In the study, the EvaGreen based real-time RT-PCR method is compared with real-time RT-PCR based on TaqMan probe, which is recommended as the diagnostic test by World Organisation for Animal Health (OIE). Detection limit, normalized fluorescence values, and sensitivity of the methods were evaluated in the analysis. The lowest limit …

Effect Of Different Doses Of Hydrated C$_{60}$ Fullerene Nanoparticles On Ram Semen Duringcool Storage, Şeyma Özer Kaya, İbrahi̇m Hali̇l Güngör, Serap Dayan Ci̇nkara, Tutku Can Acisu, Recep Hakki Koca, Serkan Ali̇ Akarsu, Coşkun Can, Aslihan Çakir, İrfan Yilmaz, Muhammed Sami̇ Halici, Seyfetti̇n Gür, Mustafa Sönmez, Gaffari̇ Türk

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the effect of different doses of hydrated C$_{60}$ fullerene (C$_{60}$HyFn) nanoparticles on ram semen during cool storage at 5 °C. Semen samples were collected from 7 healthy Akkaraman rams by artificial vagina during nonbreeding season (April) 3 times for two weeks (3 replications), and samples having good initial sperm motility (> 70 %) and count (> 2 billion / mL) were pooled and diluted with tris-egg yolk based extender at 37 °C. Then, the diluted semen samples were divided into 15 aliquots control and 14 different C60HyFn groups (25 nM, 50 nM, …

Analysis Of Some Heavy Metals (Cd And Pb) In The Şanlıurfa Province Using Feral Pigeonblood Samples, Özlem Ütme, Füsun Temamoğullari

Turkish Journal of Veterinary & Animal Sciences

Birds serve as good biomonitors in determining environmental pollution. As for indicators of environmental pollution, we detected blood heavy metal levels in 32 female and 32 male local Feral pigeons in the central districts of Şanlıurfa, located in southeastern Turkey, in one month of each season. The samples were analyzed by coupled plasma-mass spectrometry. Lead (Pb) levels were measured as 233.1 ± 25.7 μg/kg for Haliliye, 101.2 ± 29.7 μg/kg for Karaköprü, 81.3 ± 22.7 μg/kg for Eyyübiye, and 48.1 ± 27.1 μg/ kg for Suruç, whereas cadmium (Cd) levels were measured as 30.6 ± 12.6 μg/kg for Haliliye, 35.7 …

Genetic Parameter Estimates Of Fear, Growth, And Carcass Characteristics In Japanese Quail, Doğan Nari̇nç, Bariş Aybars Genç

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to estimate the heritability values of tonic immobility duration, several growth traits, and their genetic relationships with some slaughter traits. Fearfulness reaction was examined using tonic immobility (TI) response at 5 and 6 weeks of age. All birds were slaughtered at 6 weeks of age to measure carcass yield (CY), and percentage of breast (BY), leg (LY), wing (WY), abdominal fat (AFY). Heritability estimates for the TI durations and body weights measured at 5 and 6 weeks of age were 0.16, 0.20, and 0.46, 0.44, respectively. Heritability estimates for growth curve parameters and inflection …

Effects Of Dietary Guar Meal With Or Without Beta-Mannanase On The Egg Yolk Fatty Acids,Cholesterol, And Some Blood Parameters Of Laying Hens, Süleyman Çalişlar

Turkish Journal of Veterinary & Animal Sciences

In this study, the effects of adding enzyme to the diets containing guar meal (GM) on the egg yolk fatty acids, cholesterol, and some blood plasma parameters of hens were examined. This study was carried out in the chicken coop of the Experimental Animals Ethics Committee, Faculty of Agriculture. This study constitutes the second phase of the study titled ?Effects of dietary guar meal with or without beta-mannanase on performance and egg quality traits in laying hens?, previously published. The experimental period lasted 126 days. Ninety-six Lohman Brown hens at the age of 56 weeks were and kept in individual …

Options For Low-Cost Manufacturing And Safer Cell Therapies, Melis Keceli

Master's Theses

The proposed work aims to overcome the economical and feasibility-related limitations of the chimeric antigen receptor therapies by developing an artificial cell signaling pathway whose design transforms K562 cells into in vivo living vectors to synthesize therapeutic proteins upon engaging diseased cells in the treatment of ovarian cancer. There are various advantages of using K562 cells throughout this process. First, Food and Drug Administration approves the reinfusion of K562 cells into patients’ bodies. Second, K562 cells are more affordable than T lymphocytes, and finally, these cells can be easily manipulated with any desired genetic material and can keep the expression …

Of The Wide-Eyed In Uncertain Times: The Education Of Girls During The Third Reich, Ingmar D. Clausing

Master's Theses

The aim of this thesis is to describe how both the state school system and the Hitler Youth educated girls during the Third Reich. Conventional theories have suggested a number of ideological suppositions regarding the overall curricular emphasis, but this study contends that such simplistic determinations do not capture the full spread of the educational experience. This thesis asks if girls in Nazi Germany were truly just indoctrinated or if schooling had legitimate academic purposes. Based on actual textbooks that were studied by girls for every subject area, at different grade levels, and in different types of schools, as well …

Associations Between Identity And Future Parenthood Among Lgbtq+ Asian/Pacific Islander Americans, Kay Simon

Theses and Dissertations--Psychology

In recent years, the number of lesbian, gay, bisexual, transgender, and queer (and additional identities; LGBTQ+) identified people and the number of Asian Pacific Islander Americans (APIA) have been increasing in the United States (Movement Advancement Project 2019; Newport, 2018). As the individuals in these demographic groups develop over their lifespan, many will become parents and form families, but little work is currently available on the experiences of LGBTQ+ APIA individuals and future parenthood. Here, I report findings on the perceptions and importance of future parenthood, identity development, identity integration, and microaggressions and/or discrimination (as they relate to racial-ethnic and …

Physiological Stress Responding During Negative Memory Recollection And The Habitual Use Of Psychological Coping Strategies, Kyle P. Rawn

Theses and Dissertations--Psychology

Coping represents an important process for stress: how effectively one copes dictates how a person experiences stress. However, research has not yet examined how a person’s long-term coping habits are related to their physiological stress responding during a particular situation. The current study addresses this through examination of skin conductance level (SCL) trajectories and coping tendencies (i.e. habitual use of coping strategies) during an autobiographical interview. 167 college students completed questionnaires assessing their demographics and their frequency of use for fourteen coping strategies. SCL was collected while participants recalled and discussed a negative family memory from their childhood. Multilevel modeling …

Pharmacokinetics And Reward-Related Behaviors Of Ethanol In Male And Female Japanese Quail (Coturnix Japonica), Shannon Eaton

Theses and Dissertations--Psychology

Ethanol is one of the most widely used and abused drugs. Problem use is associated with many different health problems and the economic burden is in the billions of dollars. Additionally, many people have difficulty controlling their ethanol consumption and about 5% of adults end up with an alcohol use disorder (AUD). Many people with an AUD often find themselves in a cycle of binge, remission, and relapse.

Following ethanol consumption ethanol enters the bloodstream from the small intestine where it gets distributed to peripheral tissues. Ethanol in the bloodstream is cleared from the system by the liver. The primary …

Parent School Involvement, Student School Engagement, And Academic Achievement In Children Adopted From Foster Care By Lesbian And Gay Parents, Cassandra P. Vazquez

Theses and Dissertations--Psychology

Sparse research has examined academic outcomes of children adopted from foster care by lesbian and gay (LG) parents. Children who have experienced foster care are at greater risk for negative outcomes, but investment in academic achievement could help buffer potential adversity. Parent and student engagement with school peaks in middle childhood, so this may be an important period for understanding processes that influence academic achievement. This study explores how LG parents (N = 57) of school-aged children (Mage = 10.56 years) adopted from foster care in the U.S. involve themselves in their child’s school, how this involvement may …

Altering The Distance: Using Construal Level Theory To Examine Conciliatory Policy Support, Brandon Reinkensmeyer

Theses and Dissertations--Psychology

Distancing—a cognitive reappraisal strategy—increases conciliatory policy support (i.e., policies redressing inequity) by reducing anger. Related but distinct research has used construal level theory (CLT), which explains the relationship between psychological distance and mental representations (e.g., events and attitudes), to explore intergroup relations. CLT demonstrates increased psychological distance induces abstract thinking, which leads to high-level construal (HLC) mindsets. HLC mindsets, like distancing, decrease political polarization and prejudice toward out-groups. As both distancing (an appraisal technique) and HLC mindsets (a potential outcome of appraisal techniques) may decrease political polarization and prejudice toward outgroups, across two experiments, the present work investigated whether …

The Effects Of Same-Sex Parental Relationships On Teenagers, Denise A. Daniels

Theses and Dissertations

Having same-sex parents is often linked with negative effects to the well-being of children into their adult years. The reported experiences from teenagers with same-sex parents and the stressors they experience are next to nonexistent. For this study, an interpretative phenomenological analysis was conducted to understand how teenagers age 13-16 make meaning of their same-sex parents’ relationship and the stressors they experience.

The theoretical framework for this study was based on the collaborative language system (CLS) of Anderson and Goolishian. The researcher investigated the perceptions of four teenaged participants regarding their relationships with their same-sex parents and how they coped …

Russo-Ukrainian Patterns Of Genocide In The Twentieth Century, Amos Fox

Journal of Strategic Security

Russo-Ukrainian relations in the 20th Century are dominated by genocide. Using Raphel Lemkin and Martin Shaw as a guide, one finds that Russo-Ukrainian relations during the 20th Century was a long period of genocidal action, linked by periods of punctuated genocides. These genocides included several political genocides that quelled Ukrainian nationalism and independence and kept it subjugated to Soviet Russia. Soviet Russia's genocide during the 20th Century was a carryover from Imperial Russia treatment of Ukraine, the arch of which carries over into today's relations between the two countries. Understanding this long period of genocide helps make sense of the …

Ua.Fa_Charles H. Mcnutt Papers Finding Aid

Special Collections Finding Aids

The collection is comprised of research materials, class lecture notes, documents related to archaeological projects, and historic materials related to the University of Memphis administration and student life. The collection details archaeological projects related to the Southeastern United States, and includes material related to Native American Graves Protection and Repatriation Act (NAGPRA). While most of the collection will focus on the career of Dr. McNutt, there are some materials related to his education in Anthropology.

Avian Biodiversity Of The Indian River Lagoon System, Florida, Richard L. Turner

Ocean Engineering and Marine Sciences Faculty Publications

Despite claims about high avian biodiversity in the Indian River Lagoon system, previous efforts to inventory the birds have been limited in geographic extent or in habitat. Compilation of a list of birds could rely on many resources, but ones of widespread availability, greatest historical record, and highest density of sites are two citizen-science projects: the Christmas Bird Count (CBC) and eBird. Lists of species were harvested from 17 CBC sites (‘‘circles’’) back to 1910 and from 432 eBird sites (‘‘hotspots’’) since 2002 within the boundaries of the Indian River Lagoon National Estuary Program (IRLNEP). In addition to lagoon waters, …

Biodiversity Of The Indian River Lagoon System: A Cautionary Tale From The Birds, Richard L. Turner

Ocean Engineering and Marine Sciences Faculty Publications

Prevailing dogma on comparative biodiversity of the Indian River Lagoon (IRL) stems mainly from one claim about ichthyofauna and two about avifauna. The extensive network of birdwatchers, clarity of bird taxonomy, long history of the Christmas Bird Count (CBC), and burgeoning database on ebird.org make birds excellent for geographic comparison. The 1985 claim that Merritt Island CBCs are often the ‘‘most speciose count’’ in the U.S. is unfounded. CBCs there in 1970–1985 never had the highest count, even compared to the nearby Cocoa site. For the 114th CBC (2013), it ranked 11th in Florida and 44th nationally. The 1989 claim …

Il-27 Signaling In The Maintenance Of Intestinal Barrier Following Ethanol Intoxication And Burn Injury, Marisa Luck

Dissertations

Roughly half of burn patients that are hospitalized in the United States each year have detectable blood alcohol levels. Alcohol intoxication at the time of burn injury leads to worse outcomes, including longer hospital stays, increased rates of infection, and death. One of the severe consequences of intoxication and burn injury is the disruption of the gastrointestinal (GI) tract, which houses the largest reservoir of bacteria in the human body. Leakiness of the gut barrier can contribute to inflammation, infection, or sepsis from gut-derived microbes. Interleukin-27 (IL-27) is a cytokine that has shown some beneficial effects in other models of …

Comparative Studies On Biochemical And Pharmacological Profiles Of Unfractionated Heparins & Their Depolymerized Derivatives From Bovine, Ovine And Porcine Origins, Ahmed Kouta

Dissertations

Heparin and heparin related drugs have remained the anticoagulant of choice for medical and surgical indications. These drugs are comprised of poly-component sulfated chains of varying length and biophysical structure with direct and indirect pharmacological effects. Heparins produce their therapeutic effects by multiple mechanisms involving both the cellular and humoral targets. These drugs represent natural products which are isolated from mammalian tissue rich in mast cells. Currently used heparin and LMWHs are obtained from porcine mucosal tissue. Other animal sources including bovine and ovine tissues also provide additional sources for the manufacturing of heparins. While there may be microchemical and …

F Plasmids In Escherichia Coli Decrease Permissivity To Coliphage, Cesar Eber Montelongo Hernandez

Dissertations

The urinary tract contains a community of bacteria called the urinary microbiota (urobiota) thatmay be relevant to health; the genomic component of the urobiota is the urinary microbiome (urobiome). Urinary bacteria have been associated with both asymptomatic states and disease conditions, such as urinary tract infection (UTI), overactive bladder (OAB), and urge urinary incontinence (UUI). Some bacteria, such as E. coli, are considered urinary pathogens (uropathogens) but also can be commensals. Bacteriophage (phage) are ubiquitous in nature and likely shape bacterial populations in every niche; thus, phage may be one factor that modulates the urobiota. Phages have a specific host …

Equity In Secondary Schools: Building A Strong Sense Of Belonging For Students Of Color As A Foundation For Academic Success, Joaquin Stephenson

Dissertations

The problem of practice I am investigating are students who identify as Black and Latinx who are experiencing a statistically significant low sense of belonging at Riverview High School in comparison to their White and Asian counterparts, based on survey data for the past two years. Research has shown that students’ sense of belonging stem from four major factors (1) friendship nominations, (2) time on extracurricular activities, (3) bonding with teacher, and (4) perceived discrimination. By focusing on targeted programming, this study aims to determine the two solutions that can target low sense of belonging for Black and Latinx students. …

Emancipatory And Controlling: Examining The Negotiation Of Masculinity-Centered Practices By Women's And Gender Equity Center Practitioners, Ashley M. Brown

Dissertations

Initiatives to understand and transform masculinity have increasingly emerged in higher education, calling attention to the consequences of dominant, masculine gender expectations that permeate throughout college campuses. These masculinity-centered practices in higher education encompass formalized, departmental initiatives for students, faculty, and/or staff that examine topics related to masculinity through educational workshops, speaker events, professional development sessions, dialogue spaces, and other efforts. While it is often assumed that these practices support the advancement of gender equity, scholars have theorized the ways in which this work can also lead to depoliticized efforts that may, in fact, reify patriarchal and other systems of …

Peace Bodies: Women, Encampments, And The Struggle Against Nuclear Weapons During The Cold War, 1979-1992, Janette Clay

Dissertations

"Peace Bodies: Women, Encampments, and the Struggle against Nuclear Weapons during the Cold War, 1972-1992" examines the global 1980s women's peace camping movement. This study aims to explore and comprehend peace in new ways. It is specifically targeted to define peace campers' fundamental peace principles and to discover how they embodied them. This research interrogates the ways in which the peace camping movement influenced the political and cultural developments that led to nuclear de-escalation in the final years of the Cold War. The sources for this research include women's peace camp archival records, film footage and photographs, interviews, and oral …