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Preparation Of Encapsulated Konjac Glucomannan-Based Fish Oil And Its Use In High Pressure Processed Goat Milk, Siriwan Suknicom

Chulalongkorn University Theses and Dissertations (Chula ETD)

This study was divided into 3 parts. The objective of first part aims to investigate the effect of konjac glucomannan (KGM) solution (0.02-0.5%, w/w) at different pH (3, 5 and 9) on the stability between 5% fish oil- skim milk with and without casein emulsion. The second part aims to study the effects of konjac glucomannan (KGM) solution (0.02-0.5%, w/w) at different pH (3-10) on the stability of 5%fish oil-milk emulsion. And the last part aims to study the effect of high-pressure (400, 500 and 600 MPa) on the improvement of the stability of 5%fish oil-milk emulsion. The results of …

Arsenic Removal From The Contaminated Soil By Bio-Based Washing Agent In A Combined Mechanical Agitation And Ultrasonication Process, Polwatte Arachchillaya Buddhika Prabath Abeyrathne

Chulalongkorn University Theses and Dissertations (Chula ETD)

A significant amount of arsenic (As) pollution has been found globally, with several health consequences. Soil washing is a cost-effective mix of chemical and physical procedures that have been used to remove heavy metals from polluted soil. This study investigated the possibility of using a lipopeptide biosurfactant from Bacillus subtilis GY19 as a washing agent to remove As from contaminated soil. To improve the effectiveness of biosurfactants, sodium carbonate and ethylenediaminetetraacetic acid (EDTA) were employed as builders to minimize the influence of exchangeable cations in the soil. The agricultural soil utilized in the study was spiked with As at an …

การพัฒนากระแสงานเชิงคอมพิวเตอร์เพื่อการระบุอาร์เอ็นเอที่เติมพอลิอะดีนีนในโพรคาริโอตจากข้อมูลลำดับเบสอาร์เอ็นเอที่เติมพอลิยูรีดีนด้วยนาโนพอร์โดยตรง, จิรโชติ กรัณยากรณ์

Chulalongkorn University Theses and Dissertations (Chula ETD)

กระบวนการเติมพอลิอะดีนีนเป็นการดัดแปรอาร์เอ็นเอที่ปลาย 3' หลังการถอดรหัสหรือเรียกว่าหางพอลิอะดีนีน ซึ่งหางพอลิอะดีนีนมีหน้าที่ในการควบคุมเสถียรภาพของอาร์เอ็นเอและนำไปสู่การควบคุมการแสดงออกของยีน ปัจจุบันการศึกษาความยาวและหน้าที่ของหางพอลิอะดีนีนในอาร์เอ็นเอของโพรแคริโอตมีน้อยมาก เนื่องจากความยาวของหางพอลิอะดีนีนในอาร์เอ็นเอของโพรแคริโอตสั้นมาก และทำหน้าที่เป็นสัญญาณการย่อยสลายอาร์เอ็นเอ ส่งผลให้ตรวจพบได้ยาก ในการศึกษานี้ได้ทำการพัฒนาวิธีทางคอมพิวเตอร์เพื่อศึกษาหางพอลิอะดีนีนความยาวสั้นในอาร์เอ็นเอของโพรแคริโอต โดยใช้ข้อมูลอาร์เอ็นเอจากการหาลำดับเบสยุคที่สามด้วยอ็อกซ์ฟอร์ดนาโนพอร์ที่สามารถหาลำดับเบสอาร์เอ็นเอสายยาวได้โดยตรง โดยทำการปรับปรุงขั้นตอนการเตรียมไลบรารี่สำหรับการหาลำดับเบสโดยใช้เอนไซม์เพื่อสังเคราะห์พอลิยูริดินที่ปลาย 3' ของสายลำดับเบส เพื่ออนุรักษ์ความยาวหางพอลิอะดีนีนดั้งเดิมไว้ และใช้สำหรับการเชื่อมต่อกับพอลิอะดีนีนอะแดปเตอร์สำหรับการหาลำดับเบส วิธีนี้ไม่จำเป็นต้องเพิ่มปริมาณสารพันธุกรรม หรือถอดรหัสย้อนกลับ ที่อาจส่งผลต่อความยาวดั้งเดิมของหางพอลิอะดีนีน จากการศึกษาด้วยอาร์เอ็นเอสังเคราะห์ที่มีการกำหนดความยาวหางพอลิอะดีนีนไว้คงที่ พบว่าโปรแกรมสำหรับการประมาณความยาวหางพอลิอะดีนีน (nanopolish และ tailfindr) มีความคลาดเคลื่อนในการประมาณความยาวของหางพอลิอะดีนีนสายสั้น โดยมีค่าคลาดเคลื่อนสัมบูรณ์เฉลี่ยอยู่ในช่วง 0.33 ถึง 7.49 นิวคลีโอไทด์ สำหรับ nanopolish และ 6.38 ถึง 16.89 นิวคลีโอไทด์ สำหรับ tailfindr หลังจากการปรับปรุงข้อมูลด้วยการกำจัดสัญญาณไม่ปกติและใช้ข้อมูลจากการลำดับเบสเข้าช่วยพบว่าโปรแกรมสามารถหาความยาวของหางพอลิอะดีนีนสายสั้นได้ใกล้เคียงมากขึ้น โดยมีค่าคลาดเคลื่อนสัมบูรณ์เฉลี่ยลดลงอยู่ในช่วง 0.43 ถึง 7.28 นิวคลีโอไทด์ สำหรับ nanopolish และ 3.28 ถึง 5.96 นิวคลีโอไทด์ สำหรับ tailfindr และเมื่อนำวิธีการศึกษาดังกล่าวไปประยุกต์ในอาร์เอ็นเอของ Escherichia coli K-12 ได้ตรวจพบหางพอลิอะดีนีนในยีนซึ่งเคยมีรายงานจากงานวิจัยก่อนหน้า เช่น rpsO, rplQ และ ompA เป็นต้น นอกจากนี้ยังพบยีนที่มีความเป็นไปได้ที่จะมีหางพอลิอะดีนีน แต่ยังไม่มีรายงานมาก่อน เช่น apt และ ppa เป็นต้น การศึกษาความยาวหางพอลิอะดีนีนในโพรแคริโอตสามารถเชื่อมโยงกับการควบคุมการแสดงออกของยีนซึ่งจะช่วยพัฒนาองค์ความรู้ด้านการควบคุมการแสดงออกของยีนในแบคทีเรีย และสามารถนำไปประยุกต์ได้ในอนาคต

Development Of Fenofibrate/Cyclodextrin Complex Loaded Eudragit Nanoparticles For Ocular Delivery, Soe Yadanar Khin

Chulalongkorn University Theses and Dissertations (Chula ETD)

The purpose of this study was to develop Eudragit® -nanoparticles (NPs) eye drop formulations containing fenofibrate (FE) for diabetic retinopathy and age-related macular degeneration. Due to the limited aqueous solubility of FE, it may hamper the ocular bioavailability and results in low efficiency to treat such diseases. Hence, the increased solubility of FE was determined by complexation with cyclodextrins (CDs) and CD derivatives The solubility determination was evaluated by phase-solubility technique with heating method. Among CD tested, randomly methylated-b-CD (RMbCD) exhibited the best solubilizer with the highest complexation efficiency for FE. Moreover, the water-soluble polymer i.e., Soluplus® (SOL), poloxomer 407 …

Development Of Bio-Based Nanocomposites For Biosensor And Indicator Applications In Smart Food Packaging, Abdus Sobhan

Electronic Theses and Dissertations

Smart food packaging based on biosensors has been attracting more and more interest to the industrial community because of the concerns of food quality and safety. A food packaging with biosensor has a scope to enable real-time monitoring of microbial breakdown products of packaged foods. Furthermore, one of the biggest challenges in implementing biosensor for smart packaging materials is the development of bio-sensing active materials that can leverage their electrical, thermal, biodegradable and other functional properties. In this regard, nanocellulose-based activated carbon (NAC) nanocomposite was developed using the activated carbon and nanocellulose gel using the casting method with their different …

Profiling Of Fda-Approved And Clinical Trial Drugs Revealed Shared Cytotoxicity And Collateral Sensitivity In Resistant (H69ar) And Non-Resistant (H69) Small Cell Lung Cancer Cells. (Drug Repurposing In Cancer Chemotherapy), Pius Reyderg Agyemang

Electronic Theses and Dissertations

Some cancers are capable of “spitting out” drugs being fed to them, metaphorically speaking, becoming resistant to what were previously effective chemotherapeutics. In small-cell lung cancer (SCLC), an overexpression of a membrane protein (MRP1) and its transport activity can lead to chemotherapy failure. However, this study showed that certain drugs are selectively cytotoxic (exhibit collateral sensitivity) to MRP1-overexpressed SCLC (H69AR) cells. In this study, three drugs (Erlotinib, Pyrimethamine, Fludarabine) were identified to exhibit a dose-dependent collateral sensitivity on H69AR with IC50 values of ~3.5 μM, ~2 μM, and ~20 μM respectively. Halting the transport activity of the MRP1 with 25 …

Free-Living Diazotrophs And The Nitrogen Cycle In Natural Grassland Revealed By Culture Dependent And Independent Approaches, Amrit Koirala

Electronic Theses and Dissertations

Biological nitrogen fixation contributes to half of the global supply of nitrogen to the biosphere. It is carried out by a diverse group of prokaryotes called diazotrophs via the nitrogenase enzyme. Nitrogen fixation research is focused on the narrow group of symbiotic diazotrophs, and the vast majority of free-living diazotrophs which contribute significantly to fixed nitrogen are yet to be explored. The goal of this research was to access phylogeny of diazotrophs considering the most up-to-date genomic information and apply that knowledge to understand the diversity of free-living diazotrophs in a natural grassland ecosystem, both by culture dependent and independent …

Detailed Evaluation Of Cancer Sequencing Pipelines In Different Microenvironments And Heterogeneity Levels, Batuhan Kisakol, Şahi̇n Sarihan, Mehmet Ari̇f Ergün, Mehmet Baysan

Turkish Journal of Biology

The importance of next generation sequencing (NGS) rises in cancer research as accessing this key technology becomes easier for researchers. The sequence data created by NGS technologies must be processed by various bioinformatics algorithms within a pipeline in order to convert raw data to meaningful information. Mapping and variant calling are the two main steps of these analysis pipelines, and many algorithms are available for these steps. Therefore, detailed benchmarking of these algorithms in different scenarios is crucial for the efficient utilization of sequencing technologies. In this study, we compared the performance of twelve pipelines (three mapping and four variant …

Targeting The Tumor Stroma: Integrative Analysis Reveal Gata2 And Toryaip1 As Novel Prognostic Targets In Breast And Ovarian Cancer, Ömer Faruk Erceylan, Ayşe Savaş, Esra Göv

Turkish Journal of Biology

Tumor stroma interaction is known to take a crucial role in cancer growth and progression. In the present study, it was performed gene expression analysis of stroma samples with ovarian and breast cancer through an integrative analysis framework to identify common critical biomolecules at multiomics levels. Gene expression datasets were statistically analyzed to identify common differentially expressed genes (DEGs) by comparing tumor stroma and normal stroma samples. The integrative analyses of DEGs indicated that there were 59 common core genes, which might be feasible to be potential marks for cancer stroma targeted strategies. Reporter molecules (i.e. receptor, transcription factors and …

Positive Regulation Of Tnfr1 Signaling Via Sh3 Recognition Motif, Fatma Ece Çopuroğlu, Fatma Zehra Hapi̇l Zevkli̇ler, Şükran Burçak Yoldaş, Osman Ni̇dai̇ Özeş

Turkish Journal of Biology

TNF is a pleiotropic cytokine and shows its biological function by binding to its receptors called TNFR1 and TNFR2. While TNFR1 induces apoptosis by activation of caspase-8 via the "death domain", it also activates IKKα/β, MKK3/6, MKK4/7 by activation of TAK1. Although the TNFR1 signaling pathway is known by in large, it is not known how AKT and MAPKs p38, ERK1/2, and JNK1/2 are activated. The presence of a proline-rich PPAP region, (P448PAP451, a binding site for the SH3 domain-containing proteins) very close to the C-terminus promoted us to determine whether this region has any role in the TNFR1 signal …

Negative Regulation Of Diminutive Cancer Regulator Through Differentiation And Microrna Pathway Components In Drosophila Cells, Sumira Malik

Turkish Journal of Biology

Drosophila model is intensively studied for the development of cancer. The diminutive (dMyc), a homolog of the human MYC gene, is responsible for cell- apoptosis and its upregulation is responsible for determining the fate of cancerous growth in humans and Drosophila model. This work implores the requirement of dMyc and its expression as one of the major regulator of cancer with other proteins and repression of dMyc mRNA in Drosophila S2 cells. Here we report protein complex of Argonaute 1 (AGO1), Bag of marbles (Bam), and Brain tumor (Brat) proteins and not the individual factor of this complex repression of …

Compensatory Expression Regulation Of Highly Homologous Proteins Hnrnpa1 Andhnrnpa2, Yan Chang, Xiao Feng Lu, Jia Ying Qiu

Turkish Journal of Biology

Heterogeneous nuclear ribonucleoprotein (HNRNP) A1 and A2 are the most abundant HNRNPs with nearly identical functions, and play important roles in regulating gene expression at multiple levels (i.e. transcription, posttranscription, and translation). However, the expression and regulation mechanism of HNRNPA1 and A2 themselves remain unclear. In this study, the amino acid sequences of HNRNPA1 and HNRNPA2 were compared and found to have 78% and 86% homology in key functional domains. Transfection of HEK293 cells with small interfering RNA and overexpression vectors of HNRNPA1 and HNRNPA2 demonstrated that HNRNPA1 and HNRNPA2 paralogs regulate each other?s expression in a compensatory manner at …

Synthesis Of Intracellular And Extracellular Gold Nanoparticles With A Green Machine And Its Antifungal Activity, Nurbanu Gürsoy, Betül Yilmaz Öztürk, İlknur Dağ

Turkish Journal of Biology

Green synthesis method is being increasingly used in the development of safe, stable, and eco-friendly nanostructures with biological resources. In this study, extracellular and intracellular synthesis of gold nanoparticles (AuNPs) was carried out using green algae Chlorella sorokiniana Shihira & R.W. Fresh algae were isolated and identified from Musaözü Pond located in the province of Eskişehir and then extraction process were performed. Optimization studies were studied using pH value, metal salt concentration, and time parameters for extracellular synthesis and using only time parameter for intrasellular synthesis. Since more controlled and optimum conditions can be achieved in the production of AuNPs …

Il-1r1 Deficiency Impairs Liver Regeneration After 2/3 Partial Hepatectomy In Aged Mice, Deming Li, Ze Wang, Chunyan Zhang, Cunshuan Xu

Turkish Journal of Biology

Inflammation has a dual effect: it can protect the body and destroy tissue and cell as well. The purpose of this experiment was to determine the role of IL-1R1 in liver regeneration (LR) after partial hepatectomy (PH) in aged mice. The wild-type (WT, n = 36) and the IL-1R1 knockout (KO, n = 36) 24-month-old C57BL/6J mice underwent two-thirds PH; 33 WT mice underwent sham operation. Liver coefficient was calculated by liver/body weight. The mRNA and protein expressions of genes were evaluated by quantitative realtime polymerase chain reaction (qRT-PCR) and Western blotting methods, respectively. Compared with WT mice, liver coefficient …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Reconstitution Of Pol Ii (G) Responsive Form Of The Human Mediator Complex, Murat Alper Cevher

Turkish Journal of Biology

RNA polymerase II (Pol II) is a 12 subunit protein complex from yeast to human that is required for gene expression. Gdown1 containing Pol II [Pol II (G)] is a special form of Pol II that is catalytically inactive and heavily depends on the 30-subunit Mediator complex for its activator and basal dependent function in vitro. Here we report for the first time, the identification and the generation of a 15-subunit human Mediator complex via the novel multibac baculovirus expression system that is fully responsive to Pol II (G). Our results show complete recovery of Pol II (G) dependent transcription …

Polyhydroxyalkanoate Accumulation In Streptomyces Coelicolor Affected By Sco7613 Gene Region, Zeynep Demi̇r Öksüz, Tuğrul Doruk, Nevi̇n Yağci, Sedef Tunca Gedi̇k

Turkish Journal of Biology

Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, phaC gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome. BLAST search results of the GenBank database argued that SCO7613 could specify a putative polyhydroxyalkanoate synthase (PhaC) responsible for PHA biosynthesis. Deduced amino acid sequence of SCO7613 showed the presence of conserved lipase box like sequence, …

Lipase From New Isolate Bacillus Cereus Ata179: Optimization Of Production Conditions, Partial Purification, Characterization And Its Potential In The Detergent Industry, Eli̇f Demi̇rkan, Aynur Aybey Çeti̇nkaya, Maoulida Abdou

Turkish Journal of Biology

In this study, 341 Bacillus sp. strains were isolated from agricultural soils of Turkey. The potent extracellular lipase producer was selected. It was identified by 16S rRNA, named as Bacillus cereus ATA179. Optimal nutritional and physical parameters for lipase production were determined. Sucrose as the carbon source, (NH4)2HPO4 as the nitrogen source, CaCl2 as the metal ion were obtained. The best results of physical parameters were stated at 45°C, pH 7.0, shaking rate 50 rpm, inoculation amount 7% and inoculum age 24 h. ATA179 strain showed a 51% increase in enzyme production in the modified medium created by optimizing nutritional …

Transcriptome Profiling Of Stevia Rebaudiana Ms007 Revealed Genes Involved In Flower Development, Nurul Hidayah Samsulrizal, Khairul Shahyidi Khadzran, Tamil Sundram Meenakshi Sundram, Zarina Zainuddin, Siti Hajar Nor Shaarani, Nur Sabrina Ahmad Azmi, Sarahani Harun

Turkish Journal of Biology

Stevia rebaudiana is a medicinal plant recommended to diabetic or obese patients as an alternative sweetener owing to its low-calorie property. Previous studies have found that the stevioside level is highest at the time of flower bud formation and lowest at the time of preceding and following flower bud formation. Hence, this study aims to identify the genes involved in the flowering of local S. rebaudiana accession MS007 by investigating the transcriptomic data of two stages of growth, before flowering (BF) and after flowering (AF) that were deposited under accession number SRX6362785 and SRX6362784 at the NCBI SRA database. The …

Linkage Of Nanosecond Protein Motion With Enzymatic Methyl Transfer By Nicotinamide N-Methyltransferase, Yahui Jing, Yiting Cheng, Fangya Li, Yuping Li, Fan Liu, Jianyu Zhang

Turkish Journal of Biology

Nicotinamide N-methyltransferase (NNMT), a key cytoplasmic protein in the human body, is accountable to catalyze the nicotinamide (NCA) N1-methylation through S-adenosyl-L-methionine (SAM) as a methyl donor, which has been linked to many diseases. Although extensive studies have concerned about the biological aspect, the detailed mechanism study of the enzyme function, especially in the part of protein dynamics is lacking. Here, wild-type nicotinamide N-methyltransferase together with the mutation at position 20 with Y20F, Y20G, and free tryptophan were carried out to explore the connection between protein dynamics and catalysis using time-resolved fluorescence lifetimes. The results show that wild-type nicotinamide N-methyltransferase prefers …

Characteristics And Clinical Significance Of Cd163+/Cd206+M2 Mono-Macrophage In The Bladder Cancer Microenvironment, Xiangjie Kong, Ming Zhu, Zhirong Wang, Zhuoqun Xu, Jianfeng Shao

Turkish Journal of Biology

The tumor microenvironment may recruit monocytes, with a protumoral macrophage phenotype (M2) that plays an important role in solid tumor progression and metastasis. Therefore, it is necessary to understand the characteristics of these cells for cancer prevention and treatment. Bladder cancer tissue samples and paracarcinoma tissues samples were collected, and the expression of CD163+ cells in tumor tissues was observed. Then, we observed the expression of infiltrating CD45+CD14+CD163+ cell subset and analyzed the molecular expressions related to immunity and angiogenesis. C57/BL6 mice were inoculated subcutaneously, and dynamic changes of CD11b+F4/80+CD206+ mononuclear macrophages expression for tumor-bearing mice were detected. The results …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Investigation Of Neuro-Inflammatory Parameters In A Cuprizone Induced Mouse Model Of Multiple Sclerosis, Ti̇muçi̇n Avşar, Gökçe Erdem, Gökhan Terzi̇oğlu, Eda Tahi̇r Turanli

Turkish Journal of Biology

Cuprizone, copper chelator, treatment of mouse is a toxic model of multiple sclerosis (MS) in which oligodendrocyte death, demyelination and remyelination can be observed. Understanding T and B cell subset as well as their cytokines involved in MS pathogenesis still requires further scrutiny to better understand immune component of MS. The study presented here, aimed to evaluate relevant cytokines, lymphocytes, and gene expressions profiles during demyelination and remyelination in the cuprizone mouse model of MS. Eighty male C57BL/6J mice fed with 0.2% cuprizone for eight weeks. Cuprizone has been removed from the diet in the following eight weeks. Cuprizone treated …

Classification Of Bladder Cancer Cell Lines According To Regulon Activity, Aleyna Eray, Serap Erkek Özhan

Turkish Journal of Biology

Bladder cancer is one of the most frequent cancers and causes more than 150.000 deaths each year. During the last decade, several studies provided important aspects about genomic characterization, consensus subgroup definition, and transcriptional regulation of bladder cancer. Still, much more research needs to be done to characterize molecular signatures of this cancer in depth. At this point, the use of bladder cancer cell lines is quite useful for the identification and test of new signatures. In this study, we classified the bladder cancer cell lines according to the activities of regulons implicated in the regulation of primary bladder tumors. …

Fluid Shear Stress And Endothelial Cells Synergistically Promote Osteogenesis Of Mesenchymal Stem Cells Via Integrin Ss1-Fak-Erk1/2 Pathway, Mingli Jiang, Qihua Shen, Yi Zhou, Wenxia Ren, Miaomiao Chai, Yan Zhou, Wen-Song Tan

Turkish Journal of Biology

Prevascularization and mechanical stimulation have been reported as effective methods for the construction of functional bone tissue. However, their combined effects on osteogenic differentiation and its mechanism remain to be explored. Here, the effects of fluid shear stress (FSS) on osteogenic differentiation of rat bone-marrow-derived mesenchymal stem cells (BMSCs) when cocultured with human umbilical vein endothelial cells (HUVECs) were investigated, and underlying signaling mechanisms were further explored. FSS stimulation for 1-4 h/day increased alkaline phosphatase (ALP) activity and calcium deposition in coculture systems and promoted the proliferation of cocultured cells. FSS stimulation for 2 h/day was selected as the optimized …

A Cytoplasmic Escapee: Desmin Is Going Nuclear, Ecem Kural Mangit, Ni̇lüfer Düz, Pervi̇n Ruki̇ye Di̇nçer

Turkish Journal of Biology

It has been a long time since researchers have focused on the cytoskeletal proteins' unconventional functions in the nucleus. Subcellular localization of a protein not only affects its functions but also determines the accessibility for cellular processes. Desmin is a muscle-specific, cytoplasmic intermediate filament protein, the cytoplasmic roles of which are defined. Yet, there is some evidence pointing out nuclear functions for desmin. In silico and wet lab analysis shows that desmin can enter and function in the nucleus. Furthermore, the candidate nuclear partners of desmin support the notion that desmin can serve as a transcriptional regulator inside the nucleus. …

Racism In Medicine And Science, Samantha Soriano

USF St. Petersburg campus Honors Program Theses (Undergraduate)

The history of racism and prejudice in the United States is not limited to segregation, slavery, and discrimination. Medical and scientific racism continue to disproportionately affect Black and Indigenous People of Color (BIPOC). BIPOC have been used for scientific advancement without full disclosure of potential harm or adequate compensation. Scientists and medical providers have dehumanized minority individuals by disrespecting their autonomy and targeting vulnerable populations. This thesis consists of a literature review that surrounds the history of medical and scientific exploitation of people of color, ethical guidelines surrounding research, eugenics, and how assumptions affect the quality of care that minority …

Advanced Raman Spectroscopy Detection Of Oxidative Damage In Nucleic Acid Bases: Probing Chemical Changes And Intermolecular Interactions In Guanosine At Ultralow Concentration, Francesca Ripanti, Claudia Fasolato, Flavia Mazzarda, Simonetta Palleschi, Marina Ceccarini, Chunchun Li, Margherita Bignami, Enrico Bodo, Steven E.J. Bell, Filomena Mazzei, Paolo Postorino

Bioelectrics Publications

DNA/RNA synthesis precursors are especially vulnerable to damage induced by reactive oxygen species occurring following oxidative stress. Guanosine triphosphates are the prevalent oxidized nucleotides, which can be misincorporated during replication, leading to mutations and cell death. Here, we present a novel method based on micro-Raman spectroscopy, combined with ab initio calculations, for the identification, detection, and quantification of oxidized nucleotides at low concentration. We also show that the Raman signature in the terahertz spectral range (<100 >cm(-1)) contains information on the intermolecular assembly of guanine in tetrads, which allows us to further boost the oxidative damage detection limit. Eventually, we …

Coping Skills, Emotional Intelligence, Attachment, And Resilience On Post-Traumatic Stress Disorder In Private Sector Emergency Medical Technicians, Brenda-Joyce Gonzales Orozco Markert-Green

Dissertations

Problem

Private sector Emergency Medical Technicians (EMTs) are first responders who respond to traumatic incident scenes along with firefighters and police officers. However, news agencies and the public do not acknowledge private sector EMTs with the same vigor or accolade as public sector EMTs, nor are the risks to life, health, and mental well-being recognized among this population. Thus, the research literature has been sparse regarding how private sector EMTs experience PTSD, and what unique risk factors and needs may be present.

Purpose Statement

The purpose of this study was to examine the relationship between PTSD symptoms and coping skills, …

An Exploratory Study Of Mathematics Anxiety In Caribbean Preservice Teachers, Franka Atwell-Chin Hong

Dissertations

The Problem

Correlational studies suggest that gender, attitudes to mathematics, mathematics performance, the number of college mathematics courses taken, and mathematics teacher efficacy beliefs may be related to mathematics anxiety among preservice teachers (Boyd et al., 2014; Gresham, 2018; Jaggernauth & Jameson-Charles, 2015). Exploratory studies of mathematics anxiety and teacher self-efficacy among elementary teachers exist in Trinidad and Tobago (Jaggernauth 2012; Jaggernauth & Jameson-Charles, 2015). However, the differences in samples, and weakness in methodology, may make it challenging to generalize the results to the preservice teacher population. In addition, no studies have found which variable or combination of variables is …