Digital Commons Network^™

23-Valent Polysaccharide Vaccine (Ppsv23)-Targeted Serotype-Specific Identification Of Streptococcus Pneumoniae Using The Loop-Mediated Isothermal Amplification (Lamp) Method, Jiwon Lee, Youngbae Yoon, Eun Jin Kim, Donghyun Lee, Yeoungjun Baek, Chika Takano, Bin Chang, Takahiro Iijima, Paul E. Kilgore, Satoshi Hayakawa, Tomonori Hoshino, Dong Wook Kim, Mitsuko Seki

Department of Pharmacy Practice

Reports of invasive disease due to Streptococcus pneumoniae have declined since the introduction of pneumococcal conjugate vaccines (PCV7 and PCV13). The incidence of invasive diseases due to S. pneumoniae that are not addressed by the vaccines, however, has increased in children and adults, creating a global public health problem. Previously, we established the loop-mediated isothermal amplification (LAMP) method for a PCV13 serotype-specific assay. In the current study, we developed a rapid, simple, and cost-effective assay to detect serotypes in the 23-valent pneumococcal polysaccharide vaccine (PPSV23) using the LAMP method. In this study, LAMP primer sets for serotypes 2, 8, 9N, …

Regulation Of Kv11.1 Isoform Expression By Polyadenylate Binding Protein Nuclear 1, Matthew R. Stump, Rachel T. Nguyen, Rachel H. Drgastin, Delaney Search, Quiming Gong, Zhengfeng Zhou

Faculty Publications - Department of Biological & Molecular Science

The Kv11.1 voltage-gated potassium channel, encoded by the KCNH2 gene, conducts the rapidly activating delayed rectifier current in the heart. KCNH2 pre-mRNA undergoes alternative polyadenylation to generate two C-terminal Kv11.1 isoforms in the heart. Utilization of a poly(A) signal in exon 15 produces the full-length, functional Kv11.1a isoform, while intron 9 polyadenylation generates the C-terminally truncated, nonfunctional Kv11.1a-USO isoform. The relative expression of Kv11.1a and Kv11.1a-USO isoforms plays an important role in the regulation of Kv11.1 channel function. In this study, we tested the hypothesis that the RNA polyadenylate binding protein nuclear 1 (PABPN1) interacts with a unique 22 nt …

A High Affinity, Partial Antagonist Effect Of 3,4-Diaminopyridine Mediates Action Potential Broadening And Enhancement Of Transmitter Release At Nmjs, Kristine S Ojala, Scott P Ginebaugh, Man Wu, Evan W Miller, Gloria Ortiz, Manuel Covarrubias, Stephen D Meriney

Department of Neuroscience Faculty Papers

3,4-Diaminopyridine (3,4-DAP) increases transmitter release from neuromuscular junctions (NMJs), and low doses of 3,4-DAP (estimated to reach ∼1 μM in serum) are the Food and Drug Administration (FDA)-Approved treatment for neuro muscular weakness caused by Lambert-Eaton myasthenic syn drome. Canonically, 3,4-DAP is thought to block voltage-gated potassium (Kv) channels, resulting in prolongation of the pre synaptic action potential (AP). However, recent reports have shown that low millimolar concentrations of 3,4-DAP have an off-Target agonist effect on the Cav1 subtype ("L-Type") of voltage-gated calcium (Cav) channels and have speculated that this agonist effect might contribute to 3,4-DAP effects on transmitter release …

[(Wr)8Wkβa]-Doxorubicin Conjugate: A Delivery System To Overcome Multi-Drug Resistance Against Doxorubicin, Khalid Zoghebi, Hamidreza Montazeri Aliabadi, Rakesh Kumar Tiwari, Keykavous Parang

Pharmacy Faculty Articles and Research

Doxorubicin (Dox) is an anthracycline chemotherapeutic agent used to treat breast, leukemia, and lymphoma malignancies. However, cardiotoxicity and inherent acquired resistance are major drawbacks, limiting its clinical application. We have previously shown that cyclic peptide [WR]₉ containing alternate tryptophan (W) and arginine (R) residues acts as an efficient molecular transporter. An amphiphilic cyclic peptide containing a lysine (K) residue and alternative W and R was conjugated through a free side chain amino group with Dox via a glutarate linker to afford [(WR)₈WKβA]-Dox conjugate. Antiproliferative assays were performed in different cancer cell lines using the conjugate and the …

Systematic Review And Meta-Analysis Of Breast Cancer Brain Metastasis And Primary Tumor Receptor Expression Discordance, Rupesh Kotecha, Raees Tonse, Muni Rubens, Michael Mcdermott, Yazmin Odia, Haley Appel, Minesh Mehta

All Publications

Background: Change in hormone receptor (estrogen [ER] and progesterone [PR]) and/or human epidermal growth factor receptor type 2 (HER2) status during the evolutionary course of metastatic breast cancer and the effect of tumor classification subtype switching remain understudied and underappreciated in brain metastasis patients.

Methods: Using preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines, a systematic review of series published prior to April 2020 obtained from the Medline database of biopsied or resected breast cancer brain metastasis (BCBM) was performed. Weighted random effects models were used to calculate pooled estimates.

Results: 15 full-text articles were included with receptor …

Using Critical Environmental Agency To Engage Teachers In Local Watersheds Through Water Quality Citizen Science, Lacey D. Huffling, Heather C. Scott

Middle Grades & Secondary Education: Faculty Publications

This qualitative study explores teachers’ critical environmental agency (CEA) through deepening content knowledge, engaging in identity development, developing a critical consciousness of place, and moving toward civic action. We explored the meanings secondary science teachers made of an on-going professional development (PD) situated in the Okefenokee Swamp (unique ecosystem that drains to Gulf of Mexico and Atlantic Ocean) and focused on local watershed citizen science monitoring and the global implications of all water being connected. Data analyses focused on how the nineteen teachers’ experiences and meanings were leveraged to develop CEA and the constraints that restricted their CEA development. Our …

Using Critical Environmental Agency To Engage Teachers In Local Watersheds Through Water Quality Citizen Science, Lacey D. Huffling, Heather Scott

Middle Grades & Secondary Education: Faculty Publications

This qualitative study explores teachers’ critical environmental agency (CEA) through deepening content knowledge, engaging in identity development, developing a critical consciousness of place, and moving toward civic action. We explored the meanings secondary science teachers made of an on-going professional development (PD) situated in the Okefenokee Swamp (unique ecosystem that drains to Gulf of Mexico and Atlantic Ocean) and focused on local watershed citizen science monitoring and the global implications of all water being connected. Data analyses focused on how the nineteen teachers’ experiences and meanings were leveraged to develop CEA and the constraints that restricted their CEA development. Our …

Cardiac Inflammation, Oxidative Stress, Nrf2 Expression, And Coagulation Events In Mice With Experimental Chronic Kidney Disease, Abderrahim Nemmar, Suhail Al-Salam, Sumaya Beegam, Nur Elena Zaaba, Javed Yasin, Naserddine Hamadi, Badreldin H. Ali

All Works

Chronic kidney disease (CKD) is known to be associated with cardiovascular dysfunction. Dietary adenine intake in mice is also known to induce CKD. However, in this experimental model, the mechanisms underlying the cardiotoxicity and coagulation disturbances are not fully understood. Here, we evaluated cardiac inflammation, oxidative stress, DNA damage, and coagulation events in mice with adenine (0.2% w/w in feed for 4 weeks)-induced CKD. Control mice were fed with normal chow for the same duration. Adenine increased water intake, urine output, relative kidney weight, the plasma concentrations of urea and creatinine, and the urinary concentrations of kidney injury molecule-1 and …

Percutaneous Anterior Cruciate Ligament Repair With Needle Arthroscopy And Biological Augmentation, Matthew Daggett, Kyle Busch, Andrea Ferretti, Edoardo Monaco, Giorgio Bruni, Adnan Saithna

GME Publications

Recent advancements in orthopaedic devices have instilled a renewed interest in repair of the anterior cruciate ligament. Biological augmentation of the repair has also recently been investigated with the hopes of improving repair outcomes and improving biological healing. The advent of needle arthroscopy allows for potentially decreased recovery times and potentially reduced complication rates compared with traditional arthroscopy. The purpose of this article is to present a percutaneous technique to repair the anterior cruciate ligament with suture tape augmentation while also augmenting with the biological byproducts from the native effusion using needle arthroscopy.

A Bibliometric Analysis Of The Tea Quality Evaluation Using Artificial Intelligence, Amruta Bajirao Patil Research Scholar, Mrinal Rahul Bachute Ph.D Guide And Associate Professor

Library Philosophy and Practice (e-journal)

ABSTRACT: In this study, we have carried the bibliometric review of the “Tea quality evaluation using artificial intelligence”. Only the Scopus database is under consideration for this analysis. To coat all possible research approaches here we have generated the valid search queries which excludes irrelevant literature. The result analysis shows overall 602 useful papers are available on the tea quality evaluation out of which 12 papers are specifically on artificial taste perception of tea. This survey illustrates the emerging trend of quality evaluation and assurance (QEA) in tea industry and its importance. As the production of tea is huge, storage …

3d-Printed Microfluidic Droplet Generator With Hydrophilic And Hydrophobic Polymers, Chandler A. Warr, Hunter S. Hinnen, Saroya Avery, Rebecca J. Cate, Gregory P. Nordin, William G. Pitt

Faculty Publications

Droplet generation has been widely used in conventional two-dimensional (2D) microfluidic devices, and has recently begun to be explored for 3D-printed droplet generators. A major challenge for 3D-printed devices is preventing water-in-oil droplets from sticking to the interior surfaces of the droplet generator when the device is not made from hydrophobic materials. In this study, two approaches were investigated and shown to successfully form droplets in 3D-printed microfluidic devices. First, several printing resin candidates were tested to evaluate their suitability for droplet formation and material properties. We determined that a hexanediol diacrylate/lauryl acrylate (HDDA/LA) resin forms a solid polymer that …

3d-Printed Microfluidic Droplet Generator With Hydrophilic And Hydrophobic Polymers, Chandler A. Warr, Hunter S. Hinnen, Saroya Avery, Rebecca J. Cate, Gregory P. Nordin, William G. Pitt

Faculty Publications

Droplet generation has been widely used in conventional two-dimensional (2D) microfluidic devices, and has recently begun to be explored for 3D-printed droplet generators. A major challenge for 3D-printed devices is preventing water-in-oil droplets from sticking to the interior surfaces of the droplet generator when the device is not made from hydrophobic materials. In this study, two approaches were investigated and shown to successfully form droplets in 3D-printed microfluidic devices. First, several printing resin candidates were tested to evaluate their suitability for droplet formation and material properties. We determined that a hexanediol diacrylate/lauryl acrylate (HDDA/LA) resin forms a solid polymer that …

The Black Maternal Health Crisis: How To Right A Harrowing History Through Judicial And Legislative Reform, Melia Thompson-Dudiak

DePaul Journal for Social Justice

No abstract provided.

Weighted Blankets And Sleep Quality In Children With Autism Spectrum Disorders: A Single-Subject Design, Bryan M. Gee, Victoria Scharp, Amy Williams

The Open Journal of Occupational Therapy

Purpose: The purpose of the single-subject study was to explore the possible relationship between weighted blanket applications and sleep quality in children with autism spectrum disorder (ASD) and behavioral manifestations of sensory processing deficits.

Method: Two 4-year-old participants diagnosed with ASD who also experienced sleep disturbances took part in a single-subject design study. Objective sleep measures and caregiver surveys were tracked for a baseline period of 7 days followed by a 14-day weighted blanket intervention and a 7-day withdrawal phase.

Results: Caregiver reports and objective data were evaluated using visual analysis and the percentage of non-overlapping data …

Adolescents’ Daily Routines: Reliability And Validity Of The Day-Opp Questionnaire; An Exploratory Study, Yael Fogel, Hadar Gross, Moria Adler, Sara Rosenblum

The Open Journal of Occupational Therapy

Background: Maintaining a balanced and diverse daily routine is one way to ease adolescents’ transition to adulthood. The absence of tools that assess adolescents’ daily routines led to developing the Daily Activities for Youth Opportunity (DAY-Opp) Questionnaire. This research describes the development, reliability, and validity of DAY-Opp as a clinical assessment tool of adolescents’ frequency, independence, and satisfaction with daily activities.

Method: The sample of 117 typically developing adolescents (59 girls and 58 boys aged 11–19 years) divided into three age groups and completed the Hebrew version of the DAY-Opp. We statistically analyzed discriminant, concurrent, and predictive validity …

Synthesis And Evaluation Of Cellulose-Based, 1,2,3-Triazolium-Functionalized Polymerized Ionic Liquids: Thermal Transitions, Ionic Conductivities, And Morphological Properties, Rose J. Miller, Vanessa M. Smith, Stacey A. Love, Sarah M. Byron, David Salas-De La Cruz, Kevin M. Miller

Faculty & Staff Research and Creative Activity

A series of 1,2,3-triazolium-functionalized cellulose derivatives were prepared using an azide–alkyne “click” cyclization strategy, followed by quaternization. As cellulose represents an inexpensive and sustainable biomacromolecule, the ability to functionalize the backbone with an ionic liquid and generate conductive materials is of great interest. Here, three different counteranions ([Br], [OTf], and [NTf2]) were employed to explore the interplay between thermal, conductive, and morphological properties using a diverse set of techniques including nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray scattering, and dielectric relaxation spectroscopy (DRS). Functionalization of native cellulose resulted in …

Gait Simulation Of Snake Robot Based On Cpg Method, Gao Qin, Zhelong Wang, Weijian Hu, Lanying Zhao

Journal of System Simulation

Abstract: Biological snakes in nature have a variety of periodic motion patterns such as serpentine motion, linear motion and lateral motion. Gaits diversity has greatly improved the adaptability of natural snakes to complex environment. Biologists has proved that such rhythmic movements of vertebrate animals are generated by CPG (the central neural pattern generator). Special mechanical structure of a snake robot with high degree offreedom and locomotion characteristics of different gaits was considered to bulid a suitale CPG network model. Hopf oscillators were chosen as neuron models of a central pattern generator owing to their stable features. A snake robot prototype …

Medical School Watercooler Newsletter - January 15, 2021, Lindsey Lyle

Watercooler Newsletter

This is the January 15, 2021 edition of the Frederick P. Whiddon College of Medicine's newsletter - Watercooler.

Contents Include:

• Pathology faculty receive funding for breast cancer research
• Meet a Med Student: Taran Carrasco
• Mark your calendar: Grand rounds
• ‘We must all do our part’ to fulfill MLK’s vision
• Davies appointed associate dean for research at USA College of Medicine
• USA researchers use national grant to study underlying causes of lupus

January 14-15, 2021, Ohio University Board Of Trustees Meeting Minutes, Ohio University Board Of Trustees

Board of Trustees Meeting Minutes

Meeting minutes document the activities of Ohio University's Board of Trustees.

Production Of Tocotrienols In Seeds Of Cotton (Gossypium Hirsutum L.) Enhances Oxidative Stability And Offers Nutraceutical Potential, Shanmukh S. Salimath, Trevor B. Romsdahl, Anji Reddy Konda, Wei Zhang, Edgar B. Cahoon, Michael K. Dowd, Thomas C. Wedegaertner, Kater D. Hake, Kent D. Chapman

Center for Plant Science Innovation: Faculty Publications

Upland cotton (Gossypium hirsutum L.) is an economically important multi-purpose crop cultivated globally for fibre, seed oil and protein. Cottonseed oil also is naturally rich in vitamin E components (collectively known as tocochromanols), with a- and c-tocopherols comprising nearly all of the vitamin E components. By contrast, cottonseeds have little or no tocotrienols, tocochromanols with a wide range of health benefits. Here, we generated transgenic cotton lines expressing the barley (Hordeum vulgare) homogentisate geranylgeranyl transferase coding sequence under the control of the Brassica napus seed-specific promoter, napin. Transgenic cottonseeds had ~twofold to threefold increases in the accumulation of total vitamin …

Behavioral And Demographic Responses Of Mule Deer To Energy Development On Winter Range, Joseph M. Northrup, Charles R. Anderson Jr, Brian D. Gerber, George Wittemyer

Natural Resources Science Faculty Publications

Anthropogenic habitat modification is a major driver of global biodiversity loss. In North America, one of the primary sources of habitat modification over the last 2 decades has been exploration for and production of oil and natural gas (hydrocarbon development), which has led to demographic and behavioral impacts to numerous wildlife species. Developing effective measures to mitigate these impacts has become a critical task for wildlife managers and conservation practitioners. However, this task has been hindered by the difficulties involved in identifying and isolating factors driving population responses. Current research on responses of wildlife to development predominantly quantifies behavior, but …

Lessons From Rapa Nui (Easter Island, Chile) For Governance In Conditions Of Environmental Uncertainty, Carl P. Lipo, Pamela A. Mischen, Terry L. Hunt

Anthropology Datasets

No abstract provided.

Production Of Embryos And A Live Offspring Using Post Mortem Reproductive Material From Bison (Bison Bison Bison) Originating In Yellowstone National Park, Usa, Hayley M. Benham, Matthew P. Mccollum, Pauline Nol, Rebecca K. Frey, P. Ryan Clarke, Jack C. Rhyan, Jennifer P. Barfield

United States Department of Agriculture Wildlife Services: Staff Publications

Bison from Yellowstone National Park (YNP) have an important genetic history. As one of the few wild herds of bison with no evidence of cattle DNA introgression and a large enough population to maintain genetic diversity, they are considered a conservation priority for the species. Unfortunately, there is a high prevalence of the zoonotic disease brucellosis in the herd. Part of the management strategy for controlling the disease and herd size in YNP is to remove bison from the population during the winter migration out of the park. This interagency management cull provides an opportunity to collect a large number …

Green Roof Project Provides Classroom Materials For Faculty, Ohio University College Of Arts & Sciences

All Forum Articles

No abstract provided.

The Circadian Cryptochrome, Cry1, Is A Pro-Tumorigenic Factor That Rhythmically Modulates Dna Repair., Ayesha A Shafi, Chris M Mcnair, Jennifer J Mccann, Mohammed Alshalalfa, Anton Shostak, Tesa M Severson, Yanyun Zhu, Andre Bergman, Nicolas Gordon, Amy C Mandigo, Saswati N Chand, Peter Gallagher, Emanuela Dylgjeri, Talya S Laufer, Irina A Vasilevskaya, Matthew J Schiewer, Michael Brunner, Felix Y Feng, Wilbert Zwart, Karen E Knudsen

Department of Cancer Biology Faculty Papers

Mechanisms regulating DNA repair processes remain incompletely defined. Here, the circadian factor CRY1, an evolutionally conserved transcriptional coregulator, is identified as a tumor specific regulator of DNA repair. Key findings demonstrate that CRY1 expression is androgen-responsive and associates with poor outcome in prostate cancer. Functional studies and first-in-field mapping of the CRY1 cistrome and transcriptome reveal that CRY1 regulates DNA repair and the G2/M transition. DNA damage stabilizes CRY1 in cancer (in vitro, in vivo, and human tumors ex vivo), which proves critical for efficient DNA repair. Further mechanistic investigation shows that stabilized CRY1 temporally regulates expression of genes required …

Lipid Metabolism As A Targetable Metabolic Vulnerability In Colorectal Cancer, Yekaterina Y. Zaytseva

Toxicology and Cancer Biology Faculty Publications

No abstract provided.

Immune-Based Mutation Classification Enables Neoantigen Prioritization And Immune Feature Discovery In Cancer Immunotherapy., Peng Bai, Yongzheng Li, Qiuping Zhou, Jiaqi Xia, Peng-Cheng Wei, Hexiang Deng, Min Wu, Sanny K Chan, John W Kappler, Yu Zhou, Eric Tran, Philippa Marrack, Lei Yin

Articles, Abstracts, and Reports

Genetic mutations lead to the production of mutated proteins from which peptides are presented to T cells as cancer neoantigens. Evidence suggests that T cells that target neoantigens are the main mediators of effective cancer immunotherapies. Although algorithms have been used to predict neoantigens, only a minority are immunogenic. The factors that influence neoantigen immunogenicity are not completely understood. Here, we classified human neoantigen/neopeptide data into three categories based on their TCR-pMHC binding events. We observed a conservative mutant orientation of the anchor residue from immunogenic neoantigens which we termed the "NP" rule. By integrating this rule with an existing …

Artistic Expressions Of Vegan Women With Disturbed Eating Behavior And Body Image Distress, Lee Ann Thill

Expressive Therapies Dissertations

This research explores the experience of women who are vegan, and have disturbed eating behaviors (DEB) and body image distress (BID). Four participants completed a series of three art-making sessions. Participants were invited to visually explore their experience as a vegan woman with DEB/BID. They made a mixed media collage with an emphasis on layering in each session. They engaged in discussion about their process, and the final art piece’s meaning. Between sessions, researcher response art pieces were created for each participant piece, with accompanying journal reflections to engage with the ideas they explored. All participant sessions were video and …

Women's Lived Experiences With Benevolent Sexism, Sarah Schwerdel Ma, Lmhc

Counseling and Psychology Dissertations

This dissertation sought to explore the ways in which adult women make meaning of experiences of benevolent sexism in their everyday lives. By highlighting the meaning-making process of women who have experienced benevolent sexism, this research provides an important perspective on what it is like to experience gender-based oppression. Understanding that covert forms of sexism have implications for women’s well-being is an essential aspect of social-justice oriented mental health counseling. This phenomenological study focused on the perspectives of twelve adult women who have experienced benevolent sexism. Data were collected through semi-structured individual interviews. Line by line data analysis of the …

The Barley Hvwrky6 Transcription Factor Is Required For Resistance Against Pyrenophora Teres F. Teres, Prabin Tamang, Jonathan K. Richards, Shyam Solanki, Gazala Ameen, Roshan Sharma Poudel, Priyanka Deka, Karl Effertz, Shaun J. Clare, Justin Hegstad, Achintya Bezbaruah, Xuehui Li, Richard D. Horsley, Timothy L. Friesen, Robert S. Brueggeman

Faculty Publications

Barley is an important cereal crop worldwide because of its use in the brewing and distilling industry. However, adequate supplies of quality malting barley are threatened by global climate change due to drought in some regions and excess precipitation in others, which facilitates epidemics caused by fungal pathogens. The disease net form net blotch caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres (Ptt) has emerged as a global threat to barley production and diverse populations of Ptt have shown a capacity to overcome deployed genetic resistances. The barley line CI5791 exhibits remarkably effective resistance to diverse Ptt isolates …