Digital Commons Network^™

The Potential Role Of Mirnas In Sars-Cov-2 Infection Prognosis: An In-Silico Approach, Joseph Ruben Quinlan, Simone Anfossi, George A. Calin Md, Phd

Summer Experience 2021

No abstract provided.

Radiobiology Behind Dose Fractionation In Ewing Sarcoma, Rebeca J. Rodriguez, Bella S. Guerrouahen, David Flint, Keri L. Schadler

Summer Experience 2021

No abstract provided.

Oxidative Phosphorylation (Oxphos) Modulation Of Immune Response In Melanoma, Alice S. Liu, Renato Guerrieri, Michael A. Davies Md, Phd

Summer Experience 2021

No abstract provided.

Characterizing Infiltrating Monocytes In Anti-Pd-1/Ctla-4 Immunotherapy Resistant Nsclc Tumors, Karina Flores, Bertha Leticia Rodriguez, Jessica Marie Konen, Limo Chen, Don Gibbons

Summer Experience 2021

No abstract provided.

Molecular Adaptations Of Mouse Lung Endothelial Cells Exposed To Different Durations Of Laminar Shear Stress And Disturbed Flow, Viraj Nitin Govani, Riccardo Ballarò, Keri Schadler

Summer Experience 2021

No abstract provided.

Spatially Resolved Transcriptomics Identified Distinct Tumor-Stroma Crosstalk Networks Associated With Chemoresistance In Ovarian Cancer, Mansi Diwanji, Jianting Sheng, Ying Zhu, Jared Burks, Rita Siu Fee Cheng, Stephen T. Wong, Samuel C. Mok, Sammy Ferri-Borgogno

Summer Experience 2021

No abstract provided.

Fibroblast Growth Factor Receptor As A Potential Target For Eliminating Dormant Autophagic Ovarian Cancer Cells, Shafqat F. Ehsan, Janice M. Santiago-O’Farrill Ph.D, Zhen Lu Md, Robert C. Bast, Jr. Md

Summer Experience 2021

No abstract provided.

Investigating The Mechanisms Of Breast Cancer Metastatic Reactivation By Generating A New Dna Construct That Targets A Mediator Complex Subunit, Janice Oh, Seong-Yeon Bae Phd, Filippo G. Giancotti Md, Phd

Summer Experience 2021

No abstract provided.

Characterization Of Small Molecules That Degrade Yap1 Protein In Cancer, Sudili M. Fernando, Praveen Barrodia, Kunal Rai

Summer Experience 2021

No abstract provided.

Generation Of Anti-Müllerian Knockout In Chickens By Crispr/Cas9 Genome Editing To Study Sex Development, Selene Nanez, Rachel D. Mullen, Richard R. Behringer

Summer Experience 2021

No abstract provided.

Nudt5 Is A Critical Growth Regulator Of Triple-Negative Breast Cancer, Negar Koupaei, Jing Qian, William M. Tahaney, Cassandra Lien Moyer, Abhijit Mazumdar, Powel H. Brown

Summer Experience 2021

No abstract provided.

Re-Expression Of Diras3 In Kras Mutant Pancreatic And Ovarian Cancers Increases Sensitivity To Autophagy Inhibitors, Alexandre Rafael Schnell Ribeiro, Gamze B. Elcik, Zhen Lu Md, Robert C. Bast, Jr. Md

Summer Experience 2021

No abstract provided.

Insertion Of Wrmscarlet11 Into Cosa-1 Gene In C. Elegans Using Crispr, Jose Araujo, Kenneth Trimmer, Swathi Arur Phd

Summer Experience 2021

No abstract provided.

Kinetic Analysis Of Model Pet Redox Reporters Using Dynamic Bioluminescence Imaging With Biologically-Relevant Reactive Oxygen Species, Michael H. Li, Seth T. Gammon Ph.D, David Piwnica-Worms Md, Ph.D

Summer Experience 2021

No abstract provided.

Tracking Plasticity-Dependent Cell Cycle Effects, Christian Y. Beke Onana, Luigi Perelli, Giannicola Genovese

Summer Experience 2021

No abstract provided.

The 4t1 Cell Line Shows Increased Radiosensitivity When Treated With Atr Inhibitors, Ilsa Qureshi, Akanksha Acharya, Rishab Kolachina, David B. Flint, Scott J. Bright, Simona F. Shaitelman, Gabriel Sawakuchi

Summer Experience 2021

No abstract provided.

The Mitochondrial Genome Of The Spiny-Wristed Fiddler Crab, Leptuca Spinicarpa Rathbun 1900, Amber Finke, James Demastes Ph.D., J. Antonio Baeza, Carl Thurman Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

No abstract provided.

Evaluation Of Pre-Lysis Rinses To Improve Dna Yield And Purity, Joseph Correa, Emma Pellegrino, Marek K. Sliwinski Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

The widespread sampling of environmental DNA from soils has led to a fundamental shift in our understanding of Earth’s microbiome by identifying previously unknown microbes that have never been grown in the laboratory. Many of the published protocols for the extraction of environmental DNA differ based on the starting material. These differences include changes to buffer composition, detergents, and enzymatic digest. To remove this bias, we sought a standardized protocol for samples from Wind Cave National Park, which includes both above ground prairie soil and paleofill from the deepest depths of the cave system. Pre-rinsing samples with either of two …

Selecting For Durability In Bacillus Cereus/Anthracis Bacteriophages, Alexis J. Moore, Michael H. Walter Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

No abstract provided.

Curating A Global Collection Of Fiddler Crabs For The American Museum Of Natural History, Nyc, Dee Dee Pitzen, Samantha Heyer, Amber Finke, Frank H. Barnwell Ph.D., Carl L. Thurman Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

A collection of approximately 1000 jars of preserved fiddler crabs was received from Dr. F.H. Barnwell, Ecology, Evolution and Behavior, University of Minnesota–Twin Cities. It contained jars of crabs from Africa, the Americas, and the Indo-Pacific. Specimens in each jar were sorted by species and gender and then counted. An Excel® spreadsheet recorded collecting data while Google Earth® determined the GPS of each recovery site. The collection was reduced to 569 jars containing 68 species representing seven genera. The entire collection consists of 7804 crabs with 5925 males and 1879 females. New labels with up-dated information were made for each …

Soil Organic Carbon Accumulation In Restored Native Prairies Over Time [Poster], Samrin Shahnaz, Kenneth J. Elgersma Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

With the recent focus on the causes and effects of climate change, the relationship between agriculture and climate change has become an important concern. Conventional farming maximizes crop production at the expense of ecosystem services like soil carbon storage. As the human population grows, it is vital to develop practices that balance crop production and ecosystem services.

We investigated organic carbon accumulation in restored prairie soil over the course of a decade. Our goal was to determine how organic carbon levels and soil bulk density changed over time, and how that change was influenced by species diversity and soil depth. …

Measuring The Microbial Limit Of Detection For A Modified Dna Extraction Protocol, Emma Pellegrino, Marek K. Sliwinski Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Typical kit-based DNA extraction techniques only isolate ~17% of the total starting DNA from a sample. When analyzing environments with few microbial cells, such as Wind Cave National Park, South Dakota, this approach is ineffective. In this study, we developed a modified DNA extraction protocol in order to increase the limit of detection. We conducted a dilution series using known amounts of Escherichia coli over a range of one million cells (1 x 10^6) down to ten cells. DNA was isolated from each of the aliquots using the modified protocol. We used polymerase chain reactions (PCRs) followed by ethidium bromide …

Identification Of Fungi And Their Secondary Metabolites From Wind Cave [Poster], Kyle J. Biscoglia, Kirk Manfredi Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Eighteen fungal samples were isolated from samples of water collected in Wind Cave, South Dakota. Ten of the samples underwent chemical extraction, a growth inhibition test, and were analyzed using proton nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectroscopy. All eighteen of the fungal samples were able to be identified to the genus level using the ITS region of their DNA, however confident identification of the species level was more difficult to attain just looking at one portion of the sequence.

Sustainable And Biodegradable Nanocellulose Composite Materials, Jacob Scheel, Tim Kidd Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Nanocellulose is made from a mixture of cellulose powder, and a variety of different materials and liquids. Unfortunately the cost to produce nanocellulose isn’t ideal, thanks to shrinkage. The goal of my research this year was to try to make nanocellulose more cost effective, by reduce shrinkage.

Comparison Of Expiring Cp-25 And 3-Year Old Cp-42 Plantings For Monarch Habitat Quality, Schuyler Hop, Laura L. Jackson Ph.D.

Summer Undergraduate Research Program (SURP) Symposium

Monarch butterflies have experienced a population decline of more than 80% in the past two decades, driven by the emergence of Roundup Ready beans and corn varieties. This development resulted in landowners spraying herbicides and killing all other plants in their field, including milkweeds, which is the genus of plants (Asclepias) that monarch caterpillars can only feed upon. Along with this development, an initiative to reintroduce native prairie ecosystems as part of the Conservation Reserve Program (CRP). In Iowa, over 80% of the land once was home to this prairie, now less than 0.1% of this rare ecosystem …

Plasmablastic Lymphoma Of The Kidney In A Previously Undiagnosed Hiv Patient, Amrita Valluri, Adarsh Sidda, Anisha Valluri, Gurusidda Manu, Niru Nahar

Marshall Journal of Medicine

We present the case of a 61-year-old male who presented with flank pain and dysuria. A biopsy and histopathological analysis of a mass found in the left kidney led to the diagnosis of plasmablastic lymphoma (PBL). The kidney is not a commonly reported site of presentation for PBL in the current literature.^1,2 As a part of routine workup, HIV testing was ordered resulting in positive preliminary and confirmatory results. The patient denied awareness of his HIV status. Chemotherapy with uricosuric agents and antiretroviral therapy was initiated, but the patient developed tumor lysis syndrome with phosphate nephropathy leading to discontinuation …

Pgx Of Chemotherapy- Induced Peripheral Neuropathy (Cipn): Cyp2d6 Genotyping And Validation, Maira M. Mulla, Vibhuti Srivastava Phd, Irene Newsham Phd

Summer Experience 2021

School of Health Professions

Examining Disparities In Incidence Of Colorectal Cancer By Race, Ethnicity, Sex, Stage, And Site, Andrea Joyce Malabay, Kristin Primm Mph, Phd, Shine Chang Phd

Summer Experience 2021

No abstract provided.

Great Lakes, Great Books Friendship Stories, Lynette Marten Suckow

Michigan Reading Journal

No abstract provided.

Glutaminase Inhibition Radiosensitizes Non-Small Cell Lung Cancer Cells To X-Rays And Protons, Rishab Kolachina, Scott J. Bright, David Flint, Mandira Manandhar, Broderick X. Turner, Mariam Ben Kacem, Philip Jones, Jeffrey Kovacs, Michael Soth, Simona F. Shaitelman, Gabriel O. Sawakuchi

Summer Experience 2021

No abstract provided.