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Mass Spectrometry Based Metabolomics And Lipidomics., Fang Yuan

Electronic Theses and Dissertations

Metabolomics and lipidomics studies are important to understand living organisms at the molecular level. In this dissertation, I conducted various untargeted and targeted metabolomics and lipidomics studies using mass spectrometry based analytical platforms. Firstly, I investigated the androgen depletion effects on fecal metabolites in a mouse model of systemic lupus erythematosus and metabolite biomarkers for colorectal cancer using two-dimensional gas and liquid chromatography-mass spectrometry. Secondly, I investigated the relationship between gut microbiota-mediated resistance and susceptibility to malaria and the production of short-chain fatty acids using gas chromatography-mass spectrometry. Thirdly, I developed a high-throughput liquid chromatography-mass spectrometry method to profile long-chain …

"When The Voices Of Children Are Heard": Evaluating The Biological Effects Of Socioeconomic Status On Children In Postmedieval London., Ashley R. Ezzo

Electronic Theses and Dissertations

As the most vulnerable members of society, children (and their treatment) may reveal important sociocultural and socioeconomic praxes throughout human history. During the Postmedieval period, children, especially low socioeconomic status (SES) individuals, participated in the workforce. By examining the demographic distributions and paleopathological profiles of juvenile skeletal remains from high SES (St. Bride’s Crypt and Chelsea Old Church) and low SES (St. Bride’s Lower and Crossbones) Postmedieval (1700-1850 CE) London cemeteries, we can better understand how SES impacted the biological health and mortality of children. While results indicated higher mortality risk associated with low SES circumstances, most differences in pathological …

Histone Deacetylases In Alcohol Associated Liver Disease., Srineil Bodduluri

Electronic Theses and Dissertations

Alcohol abuse is endemic worldwide and there is no FDA approved treatment for Alcohol-Associated Liver Disease (ALD). Many scholars have posited that targeting Histone Deacetylases (HDAC) may be therapeutic in ALD intervention. In this study, the changes in hepatic gene expression and immune phenotyping of mice undergoing chronic plus binge alcohol exposure was examined. The characterization of relative mRNA levels for all 18 HDAC were performed. Results showed decreased expression of the genes HDAC 1,7,9,10,11 and Sirtuin enzymes (SIRT) 3,4,5,7. Other pathways related to lipid metabolism as well as systemic inflammation and hepatic injury also exhibited significant changes. These altered …

Micelles And Lipid Nanoparticles: Catalysis And Biomedical Application., Faisal Ibrahim

Electronic Theses and Dissertations

RNA-based therapeutics is a rapidly expanding field due to its enormous potential for treatment of diseases through knockdown of genes or expression of therapeutic proteins. However, due to the overall negative-charge of an RNA molecule, RNA-based therapeutics must rely on delivery systems to overcome the various biological barriers for ultimate release of an RNA payload into the cytosol. Over the past three decades, the development of lipid-based RNA delivery systems, especially lipid nanoparticles (LNPs), have been comprehensively studied due to their unique properties. LNPs represent the most widely used delivery systems for RNA-based therapeutics, as evidenced by the clinical approvals …

Room Temperature Alkali Treatment Of Rice Straw For Enhanced Enzymatic Hydrolysis., Seth Mains

Electronic Theses and Dissertations

Due to the pollution of fossil fuels due to greenhouse emissions, researchers continue to search for alternative methods that allow for the clean production of energy, fuels, and polymer fibers. A method currently under investigation is the use of lignocellulosic biomass as a solution to current problems with energy and polymer fiber production. Through the process of pretreatment, possibilities for lignocellulosic biomass expand due to improved accessibility of the cellulose and hemicellulose which can be broken down via enzymatic hydrolysis into C5 and C6 sugars such as xylose and glucose. This study investigated the fermentation of the rice straw into …

Electrical Characterization Of Phytoplankton Suspensions Using Impedance Spectroscopy., Margaret R Jett

Electronic Theses and Dissertations

Phytoplankton are closely monitored because of their important environmental role, and the impacts they have on their local ecosystems. Some phytoplankton respond to stress by producing lipids, which can be harvested for biofuels and other chemicals. Microfluidic devices have been important in improving the portability and throughput of phytoplankton characterization methods, increasing their potential for in situ use. This study utilized a small sample of phytoplankton suspended in a stagnant medium and measured the impedance response using a lab-on-chip and impedance analyzer. The individual cell characteristics were derived from the impedance response of the group. The goal was to characterize …

Standardizing New Diagnostic Tests To Facilitate Rapid Responses To The Covid-19 Pandemic, Xiao Dong

Dissertations and Theses (Open Access)

In order to enhance the data interoperability, an expeditious and accurate standardization solution is highly desirable for naming rapidly emerging novel lab tests, and thus diminishes confusion in early responses to pandemic outbreaks. This is a preliminary study to explore the roles and implementation of medical informatics technology, especially natural language processing and ontology methods, in standardizing information about emerging lab tests during a pandemic, thereby facilitating rapid responses to the pandemic. The ultimate goal of this study is to develop an informatics framework for rapid standardization of lab testing names during a pandemic to better prepare for future public …

Intersection Of Redox Chemistry And Ubiquitylation: Post-Translational Modifications Required For Maintaining Cellular Homeostasis And Neuroprotection, Emma I. Kane, Kelly L. Waters, Donald E. Spratt

Chemistry

Neurodegeneration has been predominantly recognized as neuronal breakdown induced by the accumulation of aggregated and/or misfolded proteins and remains a preliminary factor in age-dependent disease. Recently, critical regulating molecular mechanisms and cellular pathways have been shown to induce neurodegeneration long before aggregate accumulation could occur. Although this opens the possibility of identifying biomarkers for early onset diagnosis, many of these pathways vary in their modes of dysfunction while presenting similar clinical phenotypes. With selectivity remaining difficult, it is promising that these neuroprotective pathways are regulated through the ubiquitin-proteasome system (UPS). This essential post-translational modification (PTM) involves the specific attachment of …

Genome-Wide Association Study And Genomic Selection For Yield And Related Traits In Soybean, Waltram Second Ravelombola, Jun Qin, Ainong Shi, Qijian Song, Jin Yuan, Fengmin Wang, Pengyin Chen, Long Yan, Yan Feng, Tiantian Zhao, Yaning Meng, Kexin Guan, Chunyan Yang, Mengchen Zhang

Horticulture Faculty Publications and Presentations

Soybean [Glycine max (L.) Merr.] is a crop of great interest worldwide. Exploring molecular approaches to increase yield genetic gain has been one of the main challenges for soybean breeders and geneticists. Agronomic traits such as maturity, plant height, and seed weight have been found to contribute to yield. In this study, a total of 250 soybean accessions were genotyped with 10,259 high-quality SNPs postulated from genotyping by sequencing (GBS) and evaluated for grain yield, maturity, plant height, and seed weight over three years. A genome-wide association study (GWAS) was performed using a Bayesian Information and …

Photoperiod Manipulation Reveals A Light-Driven Component To Daily Patterns Of Ventilation In Male C57bl/6j Mice, Aaron A. Jones, Lauren R. Nelson, Gabriella M. Marino, Nakia A. Chappelle, Deborah A.M. Joye, Deanna M. Arble

Biological Sciences Faculty Research and Publications

Obstructive sleep apnea is a common sleep disorder that increases risk for cardiovascular disease and mortality. The severity of sleep-disordered breathing in obstructive sleep apnea patients fluctuates with the seasons, opening the possibility that seasonal changes in light duration, or photoperiod, can influence mechanisms of breathing. Photoperiod can have profound effects on internal timekeeping and can reshape metabolic rhythms in mammals. While the daily rhythm in ventilation is largely shaped by the metabolic rate, less is known about whether ventilatory rhythms are altered in accordance with metabolism under different photoperiods. Here, we investigate the relationship between ventilation and metabolism under …

Methane Emission Treatment And Potential Upcycling Using Methylomicrobium Alcaliphilum, Jace Parkinson

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Anthropogenic methane emissions are harmful to the environment and can be difficult to treat. Use of M. alcaliphilum for bioremediation of methane looks to be one of the most promising methods of reducing the negative environmental impacts of methane emissions while simultaneously providing an economic incentive. Successful bioreactor design for M. alcaliphilum based methane treatment requires biokinetic constants and microbial growth information. Methods were established for determining biokinetic constants, including growth rate, biomass yield, specific methane consumption rate, and a correlation between methane oxidation and carbon dioxide production for M. alcaliphilum. Values determined for these constants were used to …

August 2021 Library News, University Libraries

Library News

No abstract provided.

Ecophysiology Of Toxic Bloom-Forming Cyanobacteria And Their Symbionts, Dominique J. Lockwood

Masters Theses, 2020-current

Currently, there is a pernicious microbe in the genus Microcystis that is putting the world’s freshwater resources at risk. A member of the phylum Cyanobacteria, otherwise referred to as blue-green algae, Microcystis forms massive algal blooms in lakes and has the potential to produce a toxin, which does not only harm humans, but also pets, livestock, and aquatic life. As recently as 2016, in the city of Toledo, Ohio, nearly 1 million residents went without clean drinking water for an entire weekend due to one of these Microcystis blooms. However, Microcystis does not only bloom in Ohio. In fact, …

Secondary Music Educators’ Perceptions Of And Relationships With Dominant Narratives Through Personal Philosophy, Pedagogy, And Practice, Kara Levchenko

Masters Theses, 2020-current

Recent calls for contemporary change in music education demand more than just an inclusive environment that prepares artistic citizens for lifelong music after public school. Our society has experienced overwhelming shifts since the introduction of music in the public schools in 1838. Yet, dominant narratives of secondary music ensemble education (SMEE) have been performed and expected as the norm since the establishment of large ensembles (e.g., bands, choirs, and orchestras), taking the stage to perform music of the traditional Western canon. The purpose of this explanatory sequential mixed methods study was to discover ways in which secondary music ensemble teachers …

New York Paid Family Leave: Private-Corporation Employer Perspectives In Western New York, Dr. Theresa M. Roma

Education Doctoral

The purpose of this descriptive, empirical, phenomenological qualitative study, using corporate social responsibility theory, was to gain insight on 15 private employers’ experience of mandated Paid Family Leave (PFL) policy within 17 counties in Western New York. Business leaders and human resource representatives were placed in focus groups, divided by industry. New York’s mandated PFL was implemented on January 1, 2018. The policy enhanced the federal Family Medical Leave Act (FMLA) by (a) adding a paid component, (b) expanding the definition of family, and (c) providing easier eligibility for employee utilization. The empirical research showed the positive and negative outcomes …

The Design And Rationale Of A Multicenter Real-World Trial: The Southeastern Collaboration To Improve Blood Pressure Control In The Us Black Belt – Addressing The Triple Threat, Tina O Findley, Hope Northrup

Faculty, Staff and Student Publications

The incidence of congenital heart defect (CHD) has increased over the past fifty years, partly attributed to routine fetal anatomical examination by sonography during obstetric care and improvements in ultrasound technology and technique. Fetal findings on ultrasound in addition to maternal biomarkers are the backbone of first- and second-trimester screening for common genetic conditions, namely aneuploidy. Since the introduction of non-invasive prenatal testing (NIPT) using next-generation sequencing to sequence cell-free fetal DNA, the detection rate of common trisomies as well as sex chromosomal aneuploidies have markedly increased. As the use of NIPT continues to broaden, the best means of incorporating …

Zero Sympathy: Unaccompanied Minors' Rights In The Us Immigration System, Mahrukh Ali

Indiana Journal of Global Legal Studies

This note analyzes the US Government's approach to unaccompanied minors and the webs they must navigate when they are apprehended by the US immigration system. More importantly, this note calls for reformative approaches to children's rights through acknowledging the differences between adults and children while simultaneously taking their vulnerability and autonomy into account. After explaining the migrant crisis along with its implications and examining the underlying reasons fostering this movement, this note discusses the legal options available for unaccompanied minors. It draws on the shortcomings of the immigration system as the system labels unaccompanied minors as dependent children, but also …

Twists & Swirls: Caregiving & Sexualization—Femininity Construct In Gendered Migration From Nigeria To Italy, Christiana Essie Sagay

Indiana Journal of Global Legal Studies

With globalization, interconnectivity, and the possibilities of increased income through wage differentials comes the desirability to migrate for labour. Contrary to early migration theories, women are increasingly migrating for labour independent of male breadwinners. Therefore, "temporary and permanent movements are globalizing, accelerating, diversifying and feminizing." The influence of gender on migration has become so convoluted that culturally and socially constructed roles of masculinity and femininity motivate and inspire migration trends among many women migrating from Nigeria to Italy, creating a sort of gendered social agency. This piece offers an analysis of how social contexts and gendered expectations shape the desires …

Subjective Age And Inflammation Risk In Midlife Adults: Findings From The Midlife In The United States (Midus) Studies, Andree Hartanto, Nadyanna M. Majeed, Wee Qin Ng, Colin Kai Ning Chai, Verity Yu Qing Lua

Research Collection School of Social Sciences

Recent studies have suggested that subjective age—a subjective evaluation of one's own age—is a promising construct in gerontology that may contribute our understanding of risk for immune dysfunction. Nevertheless, studies documenting the association between subjective age and inflammatory biomarkers remain limited and provide mixed findings. In the present study, we revisited the relation between subjective age and systemic inflammation by utilizing a range of well-established inflammatory biomarkers (C-reactive protein, interleukin-6, fibrinogen, E-selectin, and intercellular adhesion molecule 1) through the collection of fasting blood samples before breakfast. In a large-scale dataset of midlife adults (N = 1800), we found some evidence …

Beyond The "Formidable Circle": Race And The Limits Of Democratic Inclusion In Tocqueville's Democracy In America, Christine Dunn Henderson

Research Collection School of Social Sciences

Despite his assertion that the first volume of Democracy in America (1835) would concentrate upon institutions, Tocqueville found himself finishing the draft manuscript in 1834 and unable to conclude his study without discussing race relations in the United States. In the end, he quickly penned a final chapter. That chapter—by far the book’s longest—offers “Some Considerations on the Present State and Probable Future of the Three Races That Inhabit the Territory of the United States.” Tocqueville begins the chapter by acknowledging that its subject “is American without being democratic” (DA, p. 516), and to the extent that it analyzes slavery …

The Effect Of Sub 5% Oxygen Tension On In Vitro Human Embryo Development, Bradley James Link

EVMS School of Health Professions Theses & Dissertations

Objective: To determine the impact of < 5% O₂ tension on the in vitro development of human pre-implantation embryos. It was hypothesized that the optimal O₂ tension would differ from the commonly utilized 5%, as shown by beneficial effects after the implementation of a more physiological O₂ tension such as 2% for either the partial or full duration of in vitro culture.

Aim: To conduct a randomized controlled study to determine the effect of O₂ tension during in vitro human embryo culture on the production of reactive oxygen species (ROS), apoptosis, gene expression and embryo development.

Background: The …

The Effects Of Prescribed Burning On Soil Water Infiltration Rates And Other Select Soil Physical And Chemical Properties In East Texas, Cassady Pennington Dunson, Brian P. Oswald, Kenneth Farrish, Tyson Hart

Electronic Theses and Dissertations

This study focused on whether prescribed burning affects soil physical and chemical properties, especially water infiltration, in Western Gulf Coast forests. Soil water infiltration rates were measured 1) pre-burn (before the fire), 2) post-burn (one month after the fire), and 3) at vegetation green-up (three months after the fire). Soil samples were also collected to determine the effects of prescribed burning on soil pH, bulk density, particle density, pore space, soil strength, O-horizon weight and depth (organic matter), water stable aggregates, and soil fertility. This project was conducted on two different burn intervals. The National Forests and Grasslands of Texas …

Evaluating The Contributions Of Recollection And Familiarity On Testing And Guessing Benefits In Recognition Memory, Kendal Smith

Dissertations

Completing an interpolated memory test or trying to guess non-studied information following study has yielded powerful memory benefits relative to restudy on a final memory test. Across repetitions of testing and guessing, participants may form an expectancy of an upcoming test type, and consequently, adjust their encoding of information in anticipation of the upcoming test. Research has shown that after several task repetitions, participants form an expectancy of the upcoming task type and will encode materials to match task constraints (Huff, Yates, and Balota, 2018). It is uncertain to what extent these expectancy processes aid in facilitating recollection of specific …

Design Of New Azole Molecules To Prevent Metal Leaching From Medical Implants, Ronan Mcateer

Masters

The aim of this project was to develop new coatings for titanium based implants, most particularly stents, incorporating heterocyclic functional groups in their structure that will have the potential to bind to a titanium surface and prevent or reduce metal corrosion. Many of the current coatings are chemically bound onto metallic surfaces, but this project looks at electropolymerising the coatings onto the surface of the Ti6Al4V stents. Hence, polymerising functional groups have been incorporated with azoles in the structure of the synthesised compounds. Additionally, surface characterisation and preliminary corrosion studies have been carried out on a series of coated TI6Al4V …

Effect Of Ultrasound Pressure On The Distribution Of Bovine Serum Albumin Delivered By Focused Ultrasound-Mediated Blood-Brain Barrier Opening In Cleared Mouse Brains, Yajie Liu

McKelvey School of Engineering Graduate Student Theses & Dissertations

Most common diagnosis and therapeutic methods have low effectiveness when used on brain diseases. The key obstacle is that the blood-brain barrier (BBB) prevents most drugs from entering the brain. Some strategies have been developed to improve the efficiency of drug delivery crossing BBB. Among all these strategies, focused ultrasound-mediated BBB opening (FUS-BBB Opening) stands out since it is noninvasive and can be located to the target area. Detailed studies are required on the distribution of drugs delivered by FUS-BBB opening and the effects of FUS parameters on the distribution. This thesis proposes a pipeline involving tissue clearing and lightsheet …

Double-Blind Randomized Proof-Of-Concept Trial Of Canakinumab In Patients With Covid-19 Associated Cardiac Injury And Heightened Inflammation, Paul C Cremer, Calvin C Sheng, Debasis Sahoo, Siddharth Dugar, Robier Aguillon Prada, Tom Kai Ming Wang, Ossama K Abou Hassan, Jamie Hernandez-Montfort, David A Wolinsky, Daniel A Culver, Prabalini Rajendram, Abhijit Duggal, Danielle M Brennan, Katherine E Wolski, A Michael Lincoff, Steven E Nissen, Venu Menon

Faculty, Staff and Student Publications

AIMS: In coronavirus disease 2019 (COVID-19), myocardial injury is associated with systemic inflammation and higher mortality. Our aim was to perform a proof of concept trial with canakinumab, a monoclonal antibody to interleukin-1β, in patients with COVID-19, myocardial injury, and heightened inflammation.

METHODS AND RESULTS: This trial required hospitalization due to COVID-19, elevated troponin, and a C-reactive protein concentration more than 50 mg/L. The primary endpoint was time to clinical improvement at Day 14, defined as either an improvement of two points on a seven-category ordinal scale or discharge from the hospital. The secondary endpoint was mortality at Day 28. …

Psychedelic Drugs And Atheism: Debunking The Myths, Wayne Glausser

English Faculty publications

Two recent surveys of people who took psychedelic drugs and reported “God experience encounters”, along with successful clinical trials using psychedelic therapy for depression, have given rise to public misconceptions about psychedelics and atheism. Specifically, three inferences have been drawn: (1) that the psychedelic experience tends to dissolve atheist convictions; (2) that atheist convictions, once dissolved, are replaced with traditional monotheist beliefs; and (3) that atheism and depression somehow correlate as afflictions for which psychedelic drugs offer relief. This paper argues, based on analysis of the studies and trials along with relevant supplemental evidence, that each of these popular inferences …

Lipocalin 2 Promotes Inflammatory Breast Cancer Tumorigenesis And Skin Invasion, Emilly S. Villodre, Xiaoding Hu, Richard Larson, Pascal Finetti, Kristen Gomez, Wintana Balema, Shane R. Stecklein, Ginette Santiago-Sanchez, Savitri Krishnamurthy, Juhee Song

Health & Biomedical Sciences Faculty Publications

Inflammatory breast cancer (IBC) is an aggressive form of primary breast cancer characterized by rapid onset and high risk of metastasis and poor clinical outcomes. The biological basis for the aggressiveness of IBC is still not well understood and no IBC-specific targeted therapies exist. In this study, we report that lipocalin 2 (LCN2), a small secreted glycoprotein belonging to the lipocalin superfamily, is expressed at significantly higher levels in IBC vs non-IBC tumors, independently of molecular subtype. LCN2 levels were also significantly higher in IBC cell lines and in their culture media than in non-IBC cell lines. High expression was …

Cosm Diversity & Inclusion Newsletter, Georgia Southern University

College of Science & Mathematics: News & Publications (2012-2022)

Welcome

The Diversity & Inclusion newsletter is delivered monthly via email during the fall and spring semesters and is always available on the new COSM D&I webpage. Send contributions and suggestions to any of the COSM D&I Ambassadors listed at the bottom of this newsletter and on the webpage.

Environmental Dna (Edna) Surveys For The Smalltooth Sawfish, Pristis Pectinata, In The Chandeleur Islands, Louisiana, Emma M. Humphreys

Honors Theses

The Critically Endangered smalltooth sawfish, Pristis pectinata, was historically found throughout tropical and subtropical coastal waters of the Atlantic Ocean. As a result of mortalities in fisheries and habitat degradation, they became largely restricted to southwest Florida in the U.S. and the Bahamas by the 1980s. However, recent public encounter reports of sawfish in the Florida panhandle, Mississippi, and Louisiana suggest this species is occasionally present in northern Gulf of Mexico waters. Targeted species surveys are needed to improve our understanding of the occurrence and status of this species in these waters. This research used environmental DNA (eDNA) methods …