Digital Commons Network^™

Harnessing Acoustic Streaming For Bioanalysis In Microfluidics, Xiaojun Li

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the past few decades, microfluidic technology has been developed rapidly in both fabrication methods and multifunctionality integrations, making it a powerful tool for a wider variety of biological applications. The device fabrication method has expanded from conventional polymer-based micro devices to 3D-printed micro devices, and allows multiple functions such as electric, optic, magnetic and acoustic to be integrated on a single platform. This dissertation focuses on method development of acoustic streaming-based microfluidics in bioanalysis such as immunoassay, enzyme kinetics and DNA fragmentation analysis. In this dissertation, a micromixer was developed based on acoustic streaming generated from sharp-edge structure vibration …

Effect Of Fecal Egg Count Estimated Breeding Value On Antibody Production In Katahdin Lambs, Kelsey L. Bentley

Graduate Theses, Dissertations, and Problem Reports (ETD)

With current pressures to seek out genetic solutions to combat antimicrobial resistance, genetic resistance to the gastrointestinal nematode Haemonchus contortus may reduce need for prophylactic antibiotic use. The Katahdin breed has effectively utilized post-weaning fecal egg count estimated breeding value (PFEC EBV) to reduce parasitism within their flocks, but there still remains significant opportunity for selection within breed. In a previous study, Katahdin sheep sired by low PFEC sires had greater survivability to weaning than lambs sired by high PFEC rams and preweaning was caused by other diseases than parasitism. Those data lead to the hypothesis that selection for PFEC …

“Sir, We Have The Honour Most Respectfully To Submit Our Humble Petition”: Voices In Ink And The Politics Of Petitions In Colonial Igboland, Nigeria, 1892-1960, Bright Chiazam Alozie

Graduate Theses, Dissertations, and Problem Reports (ETD)

With limited access to the traditional sources that historians typically use to document their work, it has become necessary to find new ways to explore the voices of local peoples who have been historically “denied a voice.” One way is through the study of petitions. Indeed, scholars of history have sparingly used petitions in some way while few have made systematic use of them. Even so, historians of colonial Africa have largely overlooked petitions in their studies, despite the important role that these sources played in negotiating African-European relations. This dissertation revolves around the hundreds of selected petitions, dubbed “voices …

Prioritization Framework For Advanced Systems Of Urban Roads In The Context Of Smart Cities, Jinwoong Lee

Graduate Theses, Dissertations, and Problem Reports (ETD)

The concept of a smart city has emerged as a solution to resolve various urban challenges by applying advanced systems for the functions of the city (e.g., environment, economy, infrastructure, governance, and people). Urban infrastructure with the use of modern technologies, such as Information Communication Technology and the Internet of Things, plays an essential role in enhancing the capability of the city to resolve the challenges. Particularly, urban roads supported by advanced systems with modern technologies serve as a principal bedrock to establish a smart city. Witnessing the benefits from intelligent urban roads has promoted city stakeholders to make investment …

Analysis On Combined Heat And Power, And Combined Heat And Power Hybrid Systems For Unconventional Drilling Operations, Diego G. Dranuta Ferrer

Graduate Theses, Dissertations, and Problem Reports (ETD)

The United States (U.S.) has experienced a natural gas “boom” due to the development of unconventional shale plays, but well development is energy intensive. Operations use electric drilling rigs typically powered by either three high-horsepower diesel engines (HHPDE) or three dedicated natural gas engines (DNGE) and associated generators. From a first law analysis, HHPDEs peak at 42% efficiency at full load, while DNGE peak at about 30%. Most of the fuel energy is lost as heat rejected by the exhaust and radiators. Concurrently, during cold seasons rigs utilize boilers to provide steam throughout the rig to prevent freezing and provide …

Employing Exoglycosidase And Transferase Enzymes In Capillary Nanogel Electrophoresis For The Determination Of N-Glycan Linkages And Enzyme Michaelis-Menten Constants., Lloyd Bwanali

Graduate Theses, Dissertations, and Problem Reports (ETD)

As a post translational modification protein glycosylation plays a crucial role in protein signaling, binding, kinetics and folding. In disease diagnosis and prognosis, monitoring glycosylation has been identified as a biomarker. Sialylation and sialic acid linkage in N-glycans are markers of cancers including liver, pancreatic and kidney cancer. Quantification of sialic acid linkage is analytically challenging because of the diverse linkages and the presence of heterogenous branching. A capillary electrophoresis method is reported that integrates a unique combination of enzymes and lectins to modify sialylated asparagine-linked glycans (N-glycans) in real time in the capillary so that N-glycan structures containing α2–6-linked …

Soil Microbial Nitrogen-Transforming Genes Influenced By Tree Species And Fertilization In An Appalachian Hardwood Forest, Emma G. Fox-Fogle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many forest ecosystems have been receiving elevated nitrogen (N) deposition due to human activity. Increased ecosystem N threatens soil fertility, water and air quality, and modifies the soil microbially-mediated N cycle in complex ways. The current study investigates how the abundance of key functional N cycling genes within soil bacteria is altered by N deposition and further mediated by different dominant tree species at the Fernow Experimental Forest, WV. Soils were analyzed from two watersheds, where watershed 3 (WS 3) has been receiving experimental applications of N fertilizer that are 2x historic ambient N deposition and 5x the current ambient …

Energy Policy Dynamics In The European Parliament And In The Lithuanian Seimas, Vaida Lilionyte Manthos

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study is to apply punctuated equilibrium theory to better understand the development of energy policies in the European Parliament and in the Lithuanian Parliament – Seimas. In the first empirical chapter I analyze written questions that members of the European Parliament submitted to the EU Commission to study whether members of the European Parliament respond to punctuating events such as the Fukushima nuclear accident. I also study party groupings in the European Parliament to evaluate how punctuating events alter the policy behavior and priorities of these party groupings. In the second empirical chapter I analyze whether …

Determination Of Effectiveness Of Energy Management System In Buildings, Vivash Karki

Graduate Theses, Dissertations, and Problem Reports (ETD)

Building Energy Management Systems (BEMS) are computer-based systems that aid in managing, controlling, and monitoring the building technical services and energy consumption by equipment used in the building. The effectiveness of BEMS is dependent upon numerous factors, among which the operational characteristics of the building and the BEMS control parameters also play an essential role. This research develops a user-driven simulation tool where users can input the building parameters and BEMS controls to determine the effectiveness of their BEMS. The simulation tool gives the user the flexibility to understand the potential energy savings by employing specific BEMS control and help …

Utilizing The Coughing Rat Model Of Pertussis To Improve Vaccine Efficacy, Jesse M. Hall

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pertussis, also known as whooping cough, is a respiratory disease caused by the highly contagious, Gram-negative pathogen Bordetella pertussis (Bp). Infection occurs through inhalation of aerosolized droplets containing Bp, which then colonizes ciliated epithelial cells of the respiratory tract. Here, Bp expresses toxins and virulence factors that lead to leukocyte recruitment, paroxysmal cough, and impairment of host innate responses. Currently, in developed countries, acellular pertussis vaccines (aP; DTaP; Tdap) are used to prevent Bp infection and whooping cough disease. However, we currently realize that the aP vaccine efficacy quickly wanes resulting in a reemergence of pertussis. Recent …

Grouping Algorithms For Informative Array Testing In Disease Surveillance, David Sokolov

Graduate Theses, Dissertations, and Problem Reports (ETD)

In order to maintain normal operations and prevent unnecessary morbidity and mortality during times of disease outbreak, institutions find a need to conduct frequent and widespread testing of their constituents, often under significantly limited testing resource constraints. Faced with the challenge of how best to allo- cate these limited resources to maximum effect, institutions are increasingly turning to group (or “pooled”) testing, which involves testing strategically-chosen groups of patient samples rather than individual samples, producing significant testing resource savings under certain regimes of disease prevalence. While group test- ing can be conducted without any a priori knowledge of individual disease …

Effects Of 4-Methylcyclohexanemethanol On Stress Response Pathway Regulators, In Saccharomyces Cerevisiae, Suk Lan Ser

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hydrotropes are small molecules capable of inducing liquid-liquid phase separation by altering the solubility and conformation states of organic compounds that are increasingly becoming important in organizing chemical reactions and regulating complexes. They prevent protein aggregation causing these proteins to form condensates. Mediator, a highly conserved multi-subunit complex, plays an important role in transcription. Med15, a subunit found within the tail domain of the Mediator complex, works with stress-induced transcription factors and is regulated by many kinases, including CDKs and the AMP kinase, Snf1. Living cells respond by changing molecular and cellular pathways when they are exposed to stressful conditions. …

Novel Mechanisms Of Neuronal And Hormonal Regulation Of Large Intestinal Electrolyte Transport, Andrew John Nickerson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The mammalian colon plays a vital role in maintaining overall electrolyte and fluid homeostasis in the body, and it possesses the ability to either secrete or absorb water depending upon the needs of the organism. Secretion or absorption of electrolyte ions, such as Na⁺, Cl^- and K⁺, drives the movement of water across the colonic epithelium – the inner gut lining where all transport takes place. Aberrant absorptive and/or secretory activity within the colonic epithelium is central to the pathogenesis of many gastrointestinal diseases, such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), constipation …

Getting Connected: Exploring The Role Of Sustainability Labels In Tourism Marketing Communications, Elvira Kizilova

Graduate Theses, Dissertations, and Problem Reports (ETD)

While sustainability has become an important brand differentiator in many industries, current research suggests that tourism businesses rarely use sustainability labels in their communications, e.g., on their websites or other types of advertisements.

This dissertation examines the role of sustainability labels in increasing consumer preferences for sustainable travel destinations. Drawing on social exchange theory and anthropomorphism framework, we propose that sustainability labels increase consumer visit intentions, willingness to recommend a destination to someone else, and willingness to pay price premium for the sustainable offer. These effects are mediated by perception of goal congruence and connectedness with a destination. We further …

Exploring Early Signaling Associated With Complex Stressors, Julia A. Penatzer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Humans are constantly exposed to a vast number of stressors in our everyday lives from social interactions to physical activity to chemical exposures, to name a few. These stressors have significant impacts on an individual, from the cellular to whole organismal level. The body contains an intricate communication system that elicits a number of both biological and physiological responses as it attempts to maintain homeostasis. Observing these responses to stressors, can offer insight into a number of outcomes, which can be beneficial (increasing life longevity) or detrimental (leading to a disease state). By measuring specific biological adaptations at particular time …

Targeting Calcium Homeostasis For The Treatment Of Multiple Myeloma, Osama M. Elzamzamy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multiple myeloma (MM) is a hematological malignancy characterized by the neoplastic proliferation of the plasma cells. MM is a relatively rare cancer that accounts for about 1.8% of all cancers and is the second most common hematologic malignancies, and despite the advancement from untreatable to treatable malignancy, it is yet incurable. Calcium ions (Ca²⁺) play an important role as second messengers in regulating a plethora of physiological and pathological processes, hence cytoplasmic Ca²⁺ is tightly regulated with strict spatial and temporal control to initiate, maintain, and terminate appropriate signaling pathways and phenotypes including cellular proliferation, cell cycle …

Can Parent-Child Interaction Therapy Improve Core Features Of Autism? Findings From A Comparison Of Pcit For Children With And Without Autism Spectrum Disorder, Robin C. Han

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although there is a small yet growing body of evidence supporting Parent-Child Interaction Therapy (PCIT) as an effective treatment for disruptive behaviors among children with autism spectrum disorder (ASD; Scudder et al., 2019), further study is warranted, particularly with more robust methodology (e.g., larger sample sizes, comparison groups). Furthermore, preliminary studies have demonstrated improvements in symptoms of autism following the completion of PCIT, including improvements in frequency of child verbalizations (Hansen & Shillingsburg, 2016), caregiver report of social skills and social responsiveness (Zlomke et al., 2017), time spent in pretend toy play (Lieneman et al., 2019), and shared positive affect …

Decision-Making Involvement, Social Support For Healthy Behaviors, And Nutritional Adherence In Adolescents With Cystic Fibrosis, Jennifer Kelleher

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Adolescents with cystic fibrosis (awCF) exhibit suboptimal adherence to dietary and enzyme recommendations despite the fact that eating a high calorie diet with enzymes significantly improves lung functioning. Furthermore, during adolescence teens and their caregivers begin to negotiate treatment responsibility, shifting treatment tasks to the youth. As such, it is important to understand which aspects of the complex decision-making process (e.g., expressing an opinion, asking for information from parents/adults, making joint decisions with parents) relate to better dietary adherence in adolescents with CF and how parents can best support these healthy behaviors. Thus, this study aimed to identify how …

Characterization Of Modern Ammunition And Background Profiles: A Novel Approach And Probabilistic Interpretation Of Inorganic Gunshot Residue, Korina Layli Menking-Hoggatt

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gunshot residue (GSR) can provide essential clues in gun-related investigations. The standard practice for GSR analysis uses SEM-EDS, with the capability for single particle elemental and morphological analysis. However, the method is time-consuming and based on categorical classification models without considering case circumstances. Therefore, complementary and more encompassing methods are needed to improve evidence interpretation of modern ammunition. This research aims to fill these demands by developing standard materials and alternative methods to characterize and interpret IGSR.

This study developed primer GSR (pGSR) standards from sixty discharged primers that were fully characterized by three techniques. The number of GSR particles, …

Is Care In The Air?: A Crisis Ethics Case Study Of American Airlines’ Communication During The Covid-19 Pandemic, Lily V. Hicks

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research proposes a qualitative case study of American Airlines’ communication during the first month of the COVID-19 global pandemic. Data from the airline’s press releases along with its tweets and a sample of organizational responses to those tweets are offered for proposed analysis using the lenses of Situational Crisis Communication Theory, the Applied Model of Care Considerations, and the body of crisis literature on audience coping and emotions during crises. This in-depth look at a unique communication phenomenon will be a fundamental step in examining how airline communication exhibits ethics or lack thereof and is related to people’s emotions …

Wild And Wonderful: How Both A Local And National Newspaper Framed West Virginia Leading Up To The 2016 Election, Emily Grace Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

During the 2016 Presidential election, journalists from all over the country flocked to West Virginia to try to understand the draw to then-candidate Donald Trump. There is a well-documented history of outsiders flooding the state and its surrounding Appalachian states to attempt to make sense of the current political situation, all while operating off of stereotypes and preconceived notions about the people of the Mountain State. This study aims to determine how stereotyping and the concept of framing or othering — when in-groups create out-groups — were used by a local West Virginia paper, as well as a national newspaper …

Does A Brief Mindfulness Induction Change Delay Discounting In Individuals With High Levels Of Obsessive-Compulsive Symptoms?, Cierra B. Edwards

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obsessive-compulsive disorder (OCD) is a mental illness characterized by obsessions and compulsions that cause significant distress and/or functional impairment. Delay discounting, the decline in value of a reward with the delay of its receipt, is linked to a range of maladaptive behaviors and mental disorders. Increased delay discounting is related to obsessive-compulsive disorder (OCD), reflecting that compulsive behaviors that aim to counteract obsessive thoughts are viewed as uncontrollable by the sufferer. We aimed to experimentally reduce delay discounting in individuals with high levels of obsessive-compulsive symptoms through a brief mindfulness induction. Eligible participants were randomly assigned to receive either a …

A Case Study Investigating Therapy Methods Used By Speech Language Pathologists Working With Children With Autism Spectrum Disorders, Julia Byers Van Volkenburg

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study was an in-depth examination of the experiences of five speech language pathologists (SLPs) working with children with Autism Spectrum Disorders (ASD) during language therapy sessions. This case study aimed to better understand the various therapy techniques being used by the SLPs through interviews and a reflective journal kept by the researcher. It also sought to examine the ways those therapy techniques may have included creative dramatics. Through thematic analysis, major and minor themes emerged from the interviews. The main theme of the study was the importance of individualizing therapy for students with ASD. Individualizing therapy was supported by …

A Short Film That Addresses Substance Use Disorders In Rural Communities And Strategies In Prevention And Treatment Of Addiction, Sarah R. Poteracki

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research paper and accompanying video (link to the short film is here: https://drive.google.com/drive/folders/1etDnUmEvkOds8J0EKnq1gnFO79-GggjY) offer insight into the current state of research collaboration surrounding substance use disorders in West Virginia. This professional project explores the importance of collaboration both in a research and clinical setting and in the social realm. The film was shown at the 2nd annual meeting for the Appalachian Node of the National Institute on Drug Abuse Clinical Trials Network on February 25, 2021. I interviewed 14 researchers and experts from a diverse range of disciplines who shared insights and strategies in the prevention and treatment of …

Observational Measurement Of Attachment In Toddlers With Disruptive Behavior Using The Strange Situation Procedure And Attachment Q-Set, Corey C. Lieneman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Child-caregiver attachment is an important factor in healthy child development and is often targeted by early intervention programs. To assess the efficacy of these interventions, attachment must be accurately measured across the toddler years in populations referred for treatment of externalizing behavior problems. The Strange Situation Procedure (SSP; Ainsworth et al., 1978) and Attachment Q-Set (AQS; Waters, 1987) are empirically-validated measures of child-caregiver attachment, each with unique strengths and weaknesses. Previous research has reached mixed conclusions on relations between the observer-report AQS and SSP, depending on sample and study characteristics, and a review of the literature did not produce any …

Minnesota Early Indicator And Response System Impact On Graduation Rates: An Analysis Of An Early Warning Intervention And Monitoring Systems In An Alternative Learning Center, Andrea Jean Thiner

Education

The purpose of this study was to examine the impact of the Minnesota Early Indicator Response System (MEIRS) on graduation rates, student attendance, behavior, and academic production in an alternative setting. The quantitative data was gathered using historical school and district stored data. Descriptive statistics used included frequencies, means, and standard deviations. Inferential statistics included independent sample t-tests to analyze the relationships of the differences for graduation rates prior to and during the implementation of MEIRS. Independent sample t-tests were used to analyze the differences for student data prior to MEIRS implementation and during the MEIRS implementation. Results of the …

Student Attitudes Towards Parent Involvement And Family Educational Rights And Privacy Act Of 1974, Michael Louis Paolini

Education

The purpose of this study was to explore students’ attitudes related to the Family Educational Rights and Privacy Act of 1974 (FERPA), their perceptions of parent over-involvement (POI), and the relationships thereof. A brief overview of privacy laws and FERPA are discussed. Legal cases that involve FERPA, past research of parent involvement and millennials are also explored. This study surveyed undergraduate students currently enrolled at a large public research university in the Upper Midwest (n = 537). The data analysis used descriptive and inferential statistics. Despite a reported lack of interest in FERPA, students generally reported an awareness and understanding …

Feminist Mentorship: Women Leading Men In Counselor Education, Erin Rachel Hagen

Education

This Interpretative Phenomenological Study was initiated to explore the lived experiences of mentorship pairs in counselor education doctoral studies. The research question guiding this study was: what are the qualities of the mentoring relationship between female, cisgender, feminist-oriented counselor education mentors and their male cisgender traditionally-oriented doctoral counselor education mentees? This pair was identified for exploration by noting a gap in mentorship literature that recognizes the unique relational qualities and mentorship outcomes with cross-gender pairs, and the specific influence of feminist orientation on male mentees and the mentorship process. Cross-gender mentorship is addressed in literature but dominantly refers to men …

Employee Perspectives Regarding Responses To Toxic Leadership In The Modern Workplace: A Q Methodological Study, Emily Marie Berg

Education

Currently, in the United States, four generations with four very different cultural norms are working in the workplace simultaneously. These four generations include Baby Boomers, Generation Xers, Millennials, and Generation Zers. The four generations working in the workplace at the same time may have different beliefs as to how they respond to toxic leaders. Consequently, some responses may promote toxic leadership to flourish, and other responses may suppress toxic leadership, including workplace bullying. The purpose of this study was to demonstrate the range of perceptions regarding employee responses to toxic leadership in the modern workplace. As a result, this research …

Professional Identity Development: Perceptions Of African American Female Counselor Educators' Success In Ph.D. Completion At Predominately White Institutions, Kelsey Symone Wilson

Education

Qualitative research is scant on success factors of professional identity development for female African American counselor educators, specifically those who graduated from CACREP-accredited counselor education and supervision programs at predominately White institutions (PWIs). The purpose of this qualitative study was to understand the lived experiences of female African American counselor educators who attended a CACREP-accredited counselor education and supervision program at a (PWI). This study focused on the phenomenon of success factors of professional identity development to doctoral degree completion. This study examined the lived experiences of 10 female African American counselor educators to gain a better understanding of how …