Digital Commons Network^™

Deconvolving Genomic Regulatory Heterogeneity With Self-Reporting Transposons, Arnav Moudgil

Arts & Sciences Graduate Student Theses and Dissertations

A cell’s identity is a function of the genes expressed in that cell, which are in turn regulated by transcription factors. Over the last decade, single-cell RNA sequencing (RNA-seq) has emerged as a powerful class of techniques to characterize cellular diversity in heterogeneous tissues. These methods barcode transcripts by their cell-of-origin and assign them to specific genes. The resulting high-dimensional data are further processed to reveal clusters of cells sharing transcriptional states. Annotating these clusters, based on either known or discovered marker genes, offers a glimpse into the dynamic composition of an organ or biological process. While single-cell RNA-seq excels …

Material Difficulty And Individual Difference Factors Moderate The Eyewitness Confidence-Accuracy Relationship, Wenbo Lin

Arts & Sciences Graduate Student Theses and Dissertations

Recent studies have shown that eyewitness confidence is positively associated with high identification (ID) accuracy, but some eyewitness researchers have expressed concerns regarding the reliability of this confidence-accuracy relationship in applied settings (e.g., are there circumstances or moderator variables that make this relationship less reliable?). For the present study, we considered two types of moderator variables: material difficulty (i.e., the difficulty level associated with different sets of eyewitness stimuli) and individual difference factors such as face recognition ability. Experiment 1 examined whether these moderator variables significantly impair the confidence-accuracy relationship and Experiment 2 examined whether these same moderator variables equally …

Modifying The Aspect Survey To Support The Validity Of Student Perception Data From Different Active Learning Environments, Nicole Naibert, Erin E. Shortlidge, Jack Barbera

Chemistry Faculty Publications and Presentations

Measuring students’ perceptions of active learning activities may provide valuable insight into their engagement and subsequent performance outcomes. A recently published measure, the Assessing Student Engagement in Class Tool (ASPECT), was developed to assess student perceptions of various active learning environments. As such, we sought to use this measure in our courses to assess the students’ perceptions of different active learning environments. Initial results analyzed with confirmatory factor analysis (CFA) indicated that the ASPECT did not function as expected in our active learning environments. Therefore, before administration within an introductory biology course that incorporated two types of active learning strategies, …

Amazon Reviews Using Sentiment Analysis, Ammar Rashed Hamdallah

Theses

The intense competition to attract and maintain customers online is compelling businesses to implement novel strategies to enhance the customer experiences. It is becoming necessary for companies to examine customer reviews on online platforms such as Amazon to understand better how customers rate their products and services. The purpose of this study is to investigate how companies can conduct sentiment analysis based on Amazon reviews to gain more insights into customer experiences. The dataset selected for this capstone consists of customer reviews and ratings from consumer reviews of Amazon products. Amazon product reviews enable a business to gain insights on …

Quantum Field Theories, Topological Materials, And Topological Quantum Computing, Muhammad Ilyas

Dissertations and Theses

A quantum computer can perform exponentially faster than its classical counterpart. It works on the principle of superposition. But due to the decoherence effect, the superposition of a quantum state gets destroyed by the interaction with the environment. It is a real challenge to completely isolate a quantum system to make it free of decoherence. This problem can be circumvented by the use of topological quantum phases of matter. These phases have quasiparticles excitations called anyons. The anyons are charge-flux composites and show exotic fractional statistics. When the order of exchange matters, then the anyons are called non-Abelian anyons. Majorana …

Virology: Mutagenizing The Proteolytic Cleavage Site In The Major Capsid Protein In Ssv1, Thejanee Liyanaarachchi

University Honors Theses

SSV1, spindle-shaped virus 1, is an archaeal virus with a unique shape that belongs to the family of fuselloviruses and infects hyperthermophilic archaea that thrive at 80°C and pH 3. SSV1 has two capsid proteins, VP1 and VP3, that presumably produce the lemon-shaped virion structure characteristic of fuselloviruses. The VP1 amino acid sequence is highly conserved among SSVs. Here, we investigate the importance of the proteolytic cleavage site in position E66 in VP1 by changing its glutamic acid to the other 19 amino acids. We have developed a new protocol for creating SSV1 mutants through HiFi Assembly (NEB) to increase …

Psychological Assessment And Testing, Leslie A. Miller, Ruby Daniels

Marketing Faculty Publications (Archived)

No abstract provided.

On The Variability Of Flocculated Particle Characteristics In A Shallow Estuary, G Egan, G Chang, Aj Manning, Sg Monismith, Ob Fringer

School of Biological and Marine Sciences

No abstract provided.

A Large West Antarctic Ice Sheet Explains Early Neogene Sea-Level Amplitude, J. W. Marschalek, L. Zurli, F. Talarico, De Flierdt T Van, P. Vermeesch, A. Carter, F Beny, V. Bout-Roumazeilles, F. Sangiorgi, S. R. Hemming, L. F. Pérez, F. Colleoni, J. G. Prebble, Peer Te Van, M. Perotti, A. E. Shevenell, I Browne, Dk Kulhanek, R. Levy, D Harwood, N. B. Sullivan, S. R. Meyers, E. M. Griffith, C. D. Hillenbrand, E. Gasson, M. J. Siegert, B Keisling, K. J. Licht, G. Kuhn, Jp Dodd, C. Boshuis, Santis L De, Rm Mckay, Jeanine Ash, F Beny, Im Browne, Giuseppe Cortese, Santis L De, Jp Dodd, Oliver M. Esper, Jenny A. Gales, Dm Harwood, Saki Ishino, Ba Keisling, S Kim, S Kim, Dk Kulhanek, Jan Sverre Laberg, R. Mark Leckie, Rm Mckay, J Müller, Mo Patterson, Bw Romans, Oe Romero, F. Sangiorgi, O Seki, Ae Shevenell, Sm Singh, De Sousa Im Cordeiro, St Sugisaki, T Van De Flierdt, Peer Te Van, W Xiao, Z Xiong

School of Biological and Marine Sciences

Early to Middle Miocene sea-level oscillations of approximately 40–60 m estimated from far-field records are interpreted to reflect the loss of virtually all East Antarctic ice during peak warmth2. This contrasts with ice-sheet model experiments suggesting most terrestrial ice in East Antarctica was retained even during the warmest intervals of the Middle Miocene. Data and model outputs can be reconciled if a large West Antarctic Ice Sheet (WAIS) existed and expanded across most of the outer continental shelf during the Early Miocene, accounting for maximum ice-sheet volumes. Here we provide the earliest geological evidence proving large WAIS expansions occurred during …

Limits On Parameter Estimation Of Quantum Channels, Vishal Katariya

LSU Doctoral Dissertations

The aim of this thesis is to develop a theoretical framework to study parameter estimation of quantum channels. We begin by describing the classical task of parameter estimation that we build upon. In its most basic form, parameter estimation is the task of obtaining an estimate of an unknown parameter from some experimental data. This experimental data can be seen as a number of samples of a parameterized probability distribution. In general, the goal of such a task is to obtain an estimate of the unknown parameter while minimizing its error.

We study the task of estimating unknown parameters which …

Modeling Iterative Reconstruction And Displacement Field In The Large Scale Structure, Atsuhisa Ota, Hee Jong Seo, Shun Saito, Florian Beutler

Physics Faculty Research & Creative Works

The next generation of galaxy surveys like the Dark Energy Spectroscopic Instrument and Euclid will provide datasets orders of magnitude larger than anything available to date. Our ability to model nonlinear effects in late time matter perturbations will be a key to unlock the full potential of these datasets, and the area of initial condition reconstruction is attracting growing attention. Iterative reconstruction developed by Marcel Schmittfull et al. [Phys. Rev. D 96, 023505 (2017)PRVDAQ2470-001010.1103/PhysRevD.96.023505] is a technique designed to reconstruct the displacement field from the observed galaxy distribution. The nonlinear displacement field and initial linear density field are highly correlated. …

Ward Based Goal Directed Fluid Therapy (Gdft) In Acute Pancreatitis (Gap) Trial: A Feasibility Randomised Controlled Trial [Isrctn 36077283], F Froghi, F Soggiu, F Ricciardi, C Vindrola-Padros, L Floros, D Martin, H Filipe, M Varcada, K Gurusamy, S Bhattacharya, A Fanshawe, B Dolcea, P Mathur, B Davidson

Peninsula Medical School

Background: Goal-directed fluid therapy (GDFT) reduces complications in patients undergoing major general surgery. There are no reports of cardiac output evaluation being used to optimise the fluid administration for patients with acute pancreatitis (AP) in a general surgery ward. Method: 50 patients with AP were randomised to either ward-based GDFT (n=25) with intravenous (IV) fluids administered based on stroke volume optimisation protocol or standard care (SC) (n=25), but with blinded cardiac output evaluation, for 48-hours following hospital admission. Primary outcome was feasibility. Results: 50 of 116 eligible patients (43.1%) were recruited over 20 months demonstrating feasibility. 36 (72%) completed the …

P-P06 Ward Based Goal Directed Fluid Therapy (Gdft) In Acute Pancreatitis (Gap) Trial: A Feasibility Randomised Controlled Trial [Isrctn 36077283], F Froghi, F Soggiu, F Ricciardi, C Vindrola-Padros, L Floros, D Martin, H Filipe, M Varcada, K Gurusamy, S Bhattacharya, A Fanshawe, B Dolcea, P Mathur, B Davidson

Peninsula Medical School

No abstract provided.

Dating Martian Mafic Crust; Microstructurally Constrained Baddeleyite Geochronology Of Enriched Shergottites Northwest Africa (Nwa) 7257, Nwa 8679 And Zagami, Lg Staddon, Jr Darling, Wh Schwarz, Nr Stephen, Et Al

School of Geography, Earth and Environmental Sciences

Baddeleyite (monoclinic; m-ZrO2) is a widespread accessory phase within shergottites. However, the effects of shock loading on baddeleyite U-Pb isotopic systematics, and therefore its reliability as a geochronometer within highly shocked lithologies, are less well constrained. To investigate the effects of shock metamorphism on baddeleyite U-Pb chronology, we have conducted high-resolution microstructural analysis and in-situ U-Pb isotopic measurements for baddeleyite within enriched basaltic shergottites Northwest Africa (NWA) 7257, NWA 8679 and Zagami. Electron backscatter diffraction (EBSD) analyses of baddeleyite reveal significant microstructural heterogeneity within individual thin sections, recording widespread partial to complete reversion from high-pressure (≥3.3 GPa) orthorhombic zirconia polymorphs. …

Antibody-Drug Conjugates Plus Janus Kinase Inhibitors Enable Mhc-Mismatched Allogeneic Hematopoietic Stem Cell Transplantation, Stephen P Persaud, Julie K Ritchey, Sena Kim, Sora Lim, Peter G Ruminski, Matthew L Cooper, Michael P Rettig, Jaebok Choi, John F Dipersio

Open Access Publications

Despite the curative potential of hematopoietic stem cell transplantation (HSCT), conditioning-associated toxicities preclude broader clinical application. Antibody-drug conjugates (ADCs) provide an attractive approach to HSCT conditioning that minimizes toxicity while retaining efficacy. Initial studies of ADC conditioning have largely focused on syngeneic HSCT. However, to treat acute leukemias or induce tolerance for solid organ transplantation, this approach must be expanded to allogeneic HSCT (allo-HSCT). Using murine allo-HSCT models, we show that pharmacologic Janus kinase 1/2 (JAK1/2) inhibition combined with CD45- or cKit-targeted ADCs enables robust multilineage alloengraftment. Strikingly, myeloid lineage donor chimerism exceeding 99% was achievable in fully MHC-mismatched HSCT …

Medicarpin Isolated From Radix Hedysari Ameliorates Brain Injury In A Murine Model Of Cerebral Ischemia, Chang-Ming Chern, Chung-Kuang Lu, Kuo-Tong Liou, Yea-Hwey Wang, Keng-Chang Tsai, Chia-Lin Chang, Chia-Che Chang, Yuh-Chiang Shen

Journal of Food and Drug Analysis

The development of effective post-stroke therapy is highly demanded. Medicarpin is a key active component of a famous Chinese herbal prescription used for post-stroke treatment in Taiwan; however, little is known about its biological effects and mechanisms of action. Herein, we implemented a murine model of cerebral ischemic/reperfusional injury-related stroke to elucidate medicarpin’s neuroprotective effect. In male ICR mice 24 h after stroke induction, treatment with medicarpin (0.5 and 1.0 mg/kg, i.v.) markedly enhanced the survival rates, improved moving distance and walking area coverage, reduced brain infarction, and preserved the blood-brain barrier, supporting medicarpin’s protective effect on stroke-induced injury. Immunohistochemistry …

Hyperspectral Imaging And Data Analytics For Civil Infrastructure Inspection, Genda Chen

INSPIRE Archived Webinars

In this 50-minute lecture, the fundamental concept of hyperspectral imaging is reviewed. Each hyperspectral image represents a narrow, contiguous wavelength range of an electromagnetic spectrum, which could be indicative of a chemical substance. All the images in the spectral range of a hyperspectral camera are combined to form a three-dimensional hyperspectral data cube with two spatial dimensions and one spectral dimension. A hyperspectral cube can be sampled in four ways: spatial scanning, spectral scanning, snapshot imaging, and spatio-spectral scanning. This presentation will be focused on spatial scanning from a line-scan camera. The feasibility of horizontal imaging from a synchronized hyperspectral …

Book Reviews, Florida Historical Society

Florida Historical Quarterly

FIFTY FEET IN PARADISE: THE BOOMING OF FLORIDA, by David Nolan, reviewed by Jesse Earle Bowden; NEITHER DIES NOR SURRENDERS: A HISTORY OF THE REPUBLICAN PARTY IN FLORIDA, 1867-1970, by Peter D. Klingman, reviewed by David J. Ginzl; CATHOLICISM IN SOUTH FLORIDA, 1868-1968, by Michael J. McNally, reviewed by Joseph D. Cushman, Jr.; MURDER IN MIAMI: AN ANALYSIS OF HOMICIDE PATTERNS AND TRENDS IN DADE COUNTY (MIAMI) FLORIDA, 1917-1983, by William Wilbanks, reviewed by William Maples; ARCHAEOLOGICAL TREASURE: THE SEARCH FOR NUESTRA SENORA DE ATOCHA, by R. Duncan Mathewson, reviewed by Daniel Koski-Karell; LETTERS OF DELEGATES TO CONGRESS 1774-1789, VOLUME …

Book Reviews, Florida Historical Society

Florida Historical Quarterly

FLORIDA AND THE AMERICAN MOTION PICTURE INDUSTRY 1898-1980, by Richard Alan Nelson, reviewed by Edward D. C. Campbell, Jr.; SPANISH COLONIAL FRONTIER RESEARCH, compiled and edited by Henry F. Dobyns, reviewed by John W. Griffin; HOMEWARD BOUND, A HISTORY OF THE BAHAMA ISLANDS TO 1850, by Sandra Riley, BAHAMIAN LOYALISTS AND THEIR SLAVES, by Gail Saunders, reviewed by Daniel L. Schafer; SLAVERY AND FREEDOM IN THE AGE OF THE AMERICAN REVOLUTION, edited by Ira Berlin and Ronald Hoffman, reviewed by George C. Rogers, Jr.; BLACK LIBERATION ON CUMBERLAND ISLAND IN 1815, by Mary R. Bullard, reviewed by Kenneth Coleman; THE …

Military Duty In Antebellum Florida: The Experiences Of John Henry Winder, Arch Fredric Blakey

Florida Historical Quarterly

Two Catholic priests escorted and partially carried a dying man to the gallows at the Old Capital Prison in Washington, D.C. The condemned man was Heinrich Hartmann Wirz. Branded by his executioners as the “beast of Andersonville prison,” he was hanged on November 10, 1865. He had been found guilty of conspiring to murder Union prisoners and was executed as a war criminal, the only such casualty of the Civil War.

Francis's Metallic Lifeboats And The Third Seminole War, George E. Buker

Florida Historical Quarterly

Because Joseph Francis was a good, persistent salesman and General Thomas S. Jesup was knowledgeable about Florida and its Seminole Indians, the United States Army became interested in metallic lifeboats. By December 20, 1855, when Chief Billy Bowlegs’s warriors attacked Lieutenant George Hartsuff’s men in the Big Cypress Swamp, some of Francis’s metallic lifeboats already were in Florida waters. Thus, during the Third Seminole War, the army, for the first time, used metal boats in combat operations.

Book Reviews, Florida Historical Society

Florida Historical Quarterly

THE OLDEST CITY, edited by Jean Parker Waterbury, reviewed by Eugene Lyon; SPANISH ST. AUGUSTINE: THE ARCHAEOLOGY OF A COLONIAL CREOLE COMMUNITY, By Kathleen Deagan, reviewed by John S. Otto; FORT LAUDERDALE AND BROWARD COUNTY, by Stuart B. McIver, reviewed by Thelma Peters; EDGE OF WILDERNESS: A SETTLEMENT HISTORY OF MANATEE RIVER AND SARASOTA BAY, by Janet Snyder Matthews, reviewed by Steven F. Lawson; THE PLOT TO STEAL FLORIDA: JAMES MADISON’S PHONY WAR, by Joseph Burkholder Smith, reviewed by J. Leitch Wright, Jr.; DOGS OF THE CONQUEST, by John Grier Varner and Jeanette Johnson Varner, reviewed by Amy Turner Bushnell; …

General Joseph B. Wall And Lynch Law In Tampa, Robert P. Ingalls

Florida Historical Quarterly

On Monday morning, March 6, 1882, the news spread quickly through Tampa, a normally sleepy community of some 1,000 people. During the previous night a man had broken into the home of a prominent family living just outside town. After stealing several small items, the intruder had assaulted a young woman of the family. According to subsequent press reports, the attacker had fled before actually raping the victim, but clues to his identity, including a knife left at the scene, put Sheriff D. Isaac Craft and a posse on his trail the following morning. With Tampans gripped by feelings of …

Book Reviews, Florida Historical Society

Florida Historical Quarterly

BECALMED IN THE MULLET LATITUDES, AL BURT’S FLORIDA, by Al Burt, reviewed by E. W. Carswell; FROM SCRATCH PADS AND DREAMS: A TEN YEAR HISTORY OF THE UNIVERSITY OF NORTH FLORIDA, by Daniel L. Schafer, reviewed by James P. Jones; THE PAPERS OF HENRY CLAY, VOLUME 7, SECRETARY OF STATE, JANUARY 1, 1828-MARCH 4, 1829, edited by Robert Seager II, reviewed by Edwin A. Miles; LIBERTY AND SLAVERY: SOUTHERN POLITICS TO 1860, by William J. Cooper, Jr., reviewed by F. N. Boney; THE RULING RACE, A HISTORY OF AMERICAN SLAVEHOLDERS, by James Oakes, reviewed by Julia Floyd Smith; BLACK SOUTHERNERS, …

From Orange To Green "Gold": The Roots Of The Asparagus Fern Industry In Florida, Robert D. Manning

Florida Historical Quarterly

Central Florida was a sparsely settled frontier at the end of Reconstruction. In the 1870s and 1880s, however, an influx of pioneers transformed this wilderness into a loose network of settlements. These early communities were linked by the steamships that navigated the St. Johns and Oklawaha rivers down through the chain-of-lakes— Lake Griffin, Haines Creek, Lake Eustis, Dead River, and Lake Harris.1 This river freight route, which ran from Jacksonville to Yalaha, soon became obsolete with the expansion of the railroad in the 1870s; regional development was no longer restricted by geographic proximity to navigable bodies of water. The increasing …

The Chicora Legend And Franco-Spanish Rivalry In La Florida, Paul E. Hoffman

Florida Historical Quarterly

With the first light of Monday, June 24, 1521, Pedro de Quexo and Francisco Gordillo discovered a new land which they named the Land of St. John the Baptist in honor of the saint whose feast day it was. Entering a river, later called the Jordan for the same reason, they established contact with a village or native group called “Chicora.“ Thus began the Chicora Legend, a legend that ultimately described the land of Chicora as a new Andalusia, a land abounding in timber, vines, native olive trees, Indians, pearls, and, at a distance inland, perhaps gold and silver. Flowing …

An Intrinsic Proof Of An Extension Of Itô’S Isometry For Anticipating Stochastic Integrals, Hui-Hsiung Kuo, Pujan Shrestha, Sudip Sinha

Journal of Stochastic Analysis

No abstract provided.

Partnerships And Collaboration: Working Together To Build And Achieve Disaster Recovery, Joseph Richmond, Cheryl Knight

Journal of Global Awareness

On May 22, 2011, an EF-5 tornado struck Joplin, Missouri, leaving behind 161 fatalities and $2.8 billion in economic impacts. This case study research design used in-depth semi-structured, one-on-one interviews and a qualitative design and analysis to examine the economic recovery following the disaster. It also formed the foundation for future research on the impact of interdisciplinary teams, specifically disaster emergency management and social work in disaster recovery.

Lessons Learned From Bp: Deepwater Horizon And The Transition To Renewables, Daniel Valle

Journal of Global Awareness

This paper analyzes the gradual transition of British Petroleum (BP), one of the world's largest oil and gas companies, into a renewable energy company focused on sustainability and the reduction of carbon emissions. BP's leadership and ethical practices are compared before and after the 2010 Deepwater Horizon disaster. The purpose of the comparison and the broader analysis of the transition is to identify how effective leadership can be used to transform a company with a suspect social responsibility record into a leader among its peers. Lessons learned from the disaster, and the subsequent transition conclude the research.

We Hold These Distruths To Be Self-Evident: How Legal History Could Save America From Itself, William Murphy

Journal of Global Awareness

This paper explores the threat of disinformation to American society. Focusing on social and cable news media as the primary disseminators of disinformation, it examines how the very design and nature of these two mediums promote and stimulate the intentional and viral spread of wholly inaccurate information as well as the significant and tangible negative consequences experienced across American society as a result. The paper subsequently proposes a legal solution to this dilemma in the form of a repeal of Section 230 paired with a reinstatement of the Fairness Doctrine. The background, history, and effect of each are thoroughly discussed, …