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Characterization Of Ovarian Tissue Culture And Investigation Of Embryonic Response To Temperature Stress Using A Shp2 Phosphatase Inhibitor In Ciona Intestinalis, Rose Jacobson

Open Access Dissertations

This dissertation is comprised of two parts, the first is cell culture. In this work I describe the successful primary culture of Ciona intestinalis ovary tissue. Improved cell proliferation in 3D Petri Dish® system over culture treated plastic is shown, as well as the efficacy of using C. intestinalis hemolymph as a media additive. The seeded ovarian cells assemble into multicellular aggregations, which mimic the morphology of in vivo ovaries. It is also important to understand the molecular pathways in which marine organisms handle stress and how these pathways change in high stress situations.

The second is the role of …

Fantasizing Reproduction: The Biologization Of The Desire For Progress In Victorian Literature, Xinqiang Chang

Open Access Dissertations

The preoccupation with reproduction in Victorian literature has, by and large, received little critical attention. This dissertation seeks to contribute to Victorian scholarship and examines the representations of reproduction in Victorian literature in relation to the contemporaneous desire for progress. Adopting a historiographical approach, this dissertation analyzes five Victorian novels: Charlotte Brontë’s Jane Eyre, George Eliot’s Middlemarch, George Du Maurier’s Trilby, Thomas Hardy’s Jude the Obscure, and Sarah Grand’s The Heavenly Twins. In this dissertation, I demonstrate the different ways in which these Victorian novelists explored, accommodated, and concretized the Victorian desire for and pursuit …

Social Justice And Evolutionary Biology: A Natural Fit, David I. Upegui

Open Access Dissertations

This dissertation is an extended prescription for the inclusion of social justice in high school biology (HSB) curricula. Chapter 1 is context: a review of social injustice (mainly racism) in the US, and the specious scientific justifications for it. Chapter 2 explores how three veteran teachers introduce social justice in HSB classes. A case-study, semi-constructed interview-based approach is used. Chapter 3, the culmination of these ideas, adduces a practical curriculum module that provides the theory, lessons and activities for HSB student exploration the inferior science used to sustain racial inequities. The dissertation thus reviews the moral imperatives for introducing social …

The Role Of Climate Change In The Decline Of Narragansett Bay Winter Flounder, Joseph A. Langan

Open Access Dissertations

Winter flounder (Pseudopleuronectes americanus) has historically supported productive commercial and recreational fisheries throughout its range in the northwest Atlantic. As a small flatfish that inhabits estuaries, bays, and the coastal zone, this species is vulnerable to shifting environmental conditions in many of the habitats critical to its life cycle. At the southern edge of the species range, the Southern New England/Mid-Atlantic Bight winter flounder stock entered a steep decline during the 1980s following a period of overfishing. Despite repeated reductions in harvest during the proceeding decades, the population has yet to recover. A decreasing trend in recruitment has …

Evaluating Natural Product Libraries With Emphasis On In Vitro Permeability Workflows, Riley D. Kirk

Open Access Dissertations

New active pharmaceutical ingredients (APIs) continue to be discovered from the natural world. These compounds can be further developed into a range of useful products such as drugs, supplements, or cosmeceuticals. To determine the biological target of the API, the new compounds are evaluated for their biological activity through a combination of in silico, in vitro, and in vivo analyses. These results will determine where the molecular target tissue is located for the API. However, identification of the target is only the beginning of pharmaceutical and cosmeceutical development. A common roadblock in the development of products is the evaluation and …

Convalescent Plasma Therapy For Covid-19: A Graphical Mosaic Of The Worldwide Evidence, Stephen A Klassen, Brenda J Grossman, Jeffrey P Henderson, Et Al.

2020-Current year OA Pubs

Convalescent plasma has been used worldwide to treat patients hospitalized with coronavirus disease 2019 (COVID-19) and prevent disease progression. Despite global usage, uncertainty remains regarding plasma efficacy, as randomized controlled trials (RCTs) have provided divergent evidence regarding the survival benefit of convalescent plasma. Here, we argue that during a global health emergency, the mosaic of evidence originating from multiple levels of the epistemic hierarchy should inform contemporary policy and healthcare decisions. Indeed, worldwide matched-control studies have generally found convalescent plasma to improve COVID-19 patient survival, and RCTs have demonstrated a survival benefit when transfused early in the disease course but …

"A Code Red For Humanity": Judicial Relevance In A Time Of Climate Emergency, Margot Young

All Faculty Publications

The Washington Post calls it the “the biggest political story in the world, a grinding global crisis in public view.” The Globe and Mail’s coverage is more muted, but its Editorial Board notes about this crisis that “[t]wo things have since changed: urgency and ability. The danger is growing closer, but so is humanity’s capacity to avert disaster.” These statements reference the 9 August 2021 release by the Intergovernmental Panel on Climate Change (IPCC) of its Working Group I report, first instalment of the IPCC’s Sixth Assessment due out in 2022. The recent report, compiled by 234 authors based on …

Starting With Life: Murder Sentencing And Feminist Prison Abolitionist Praxis, Debra Parkes

All Faculty Publications

Advocates of decarcation often focus their critiques on imprisonment for non-violent offences. In this vein, current advocacy efforts to end mandatory sentences in Canada tend to carve out “serious violent offences” as not part of a reform agenda. In this chapter, Debra Parkes sketches out the contours of an argument for why feminists might not want to cede that ground, why anti-carceral feminism might involve centering our analysis on the most, rather than the least, serious crimes – starting with those who are serving life sentences for murder. Parkes identifies four non-exhaustive reasons for that focus. The first reason relates …

Embedded Property, Douglas C. Harris

All Faculty Publications

The institution of property arises in the tension between autonomy and community. It serves not simply to demarcate spaces of individual control and authority, but also to balance individual with collective interests. Private property and common property emphasize individual and collective interests, respectively, but the bifurcation may not be as stark as it appears. Condominium constructs separate titles to individual units, and these private interests are carefully mapped in a constituting plan that marks their boundaries. Democratic rights, usually conveyed in the form of shares in a condominium corporation, are the third element of ownership within condominium. The analysis reveals …

Revised Draft Final Butte Reclaimed Areas Maintenance And Monitoring (M&M) Plan In Accordance With Butte Reclamation Evaluation System (Bres), Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Draft Final Solid Media Management Program Plan, Pioneer Technical Services, Inc.

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Homotopic Functional Connectivity Disruptions In Glioma Patients Are Associated With Tumor Malignancy And Overall Survival, Andy G S Daniel, Carl D Hacker, John J Lee, Donna Dierker, Joseph B Humphries, Joshua S Shimony, Eric C Leuthardt

2020-Current year OA Pubs

BACKGROUND: Gliomas exhibit widespread bilateral functional connectivity (FC) alterations that may be associated with tumor grade. Limited studies have examined the connection-level mechanisms responsible for these effects. Given the typically strong FC observed between mirroring/homotopic brain regions in healthy subjects, we hypothesized that homotopic connectivity (HC) is altered in low-grade and high-grade glioma patients and the extent of disruption is associated with tumor grade and predictive of overall survival (OS) in a cohort of

METHODS: We used a mirrored FC-derived cortical parcellation to extract blood-oxygen-level-dependent (BOLD) signals and to quantify FC differences between homotopic pairs in normal-appearing brain in a …

Variations In Anterior Segment Vasculature Of The Eye And Their Implication In Strabismus Surgery And Other Surgical Procedures, Adam L. Lewis, Clayton Marolt, M. Scott Draper, Victoria Gordon, Kenneth S. Johnson, Joanna Rowe, Kanwalgeet Hans, Steven M. Silverstein, Anthony Olinger

Faculty and Staff Publications

Objective:

Anterior segment ischemia (ASI) is a serious complication of strabismus surgery and other ocular procedures. The purpose of this study is to evaluate variation of the long posterior artery (LPCA). These variations may predispose patients to anterior segment ischemia following strabismus surgery, and proper mapping may provide surgical “danger zones” for other invasive ocular procedures.

Subjects:

A total of 100 preserved cadaveric eyeballs from University of Nebraska Medical Center (UNMC), Creighton University, and Kansas City University (KCU) were utilized for this study.

Methods:

LPCAs were identified, isolated, and counted prior to removal of the choroid. Two distances were measured …

Contracts On The Seabed, Christiana Ochoa

Articles by Maurer Faculty

Four million square kilometers of seabed within the sovereignty of Pacific Island nations are currently under contract for mineral exploration or exploitation. Over a million additional square kilometers of the non-sovereign seafloor are licensed for such use. Historically, these licenses have served to establish “squatters’ rights” in anticipation of a distant future when the industry would develop the machinery to exploit oceanic mineral wealth. That moment has arrived, with the first seafloor mining machines rolling off production lines in 2015-2016. Indeed, but for failed financing, the first seabed mine would now be operating in the territorial ocean waters of Papua …

Judicial Independence At Twilight, Charles G. Geyh

Articles by Maurer Faculty

Judicial independence is a fixture of American government, but its structure has never been fully understood. As long as the federal judiciary has survived episodic attacks with its independence intact, there has been no pressing need to know how or why. But a confluence of cyclical, sustained, and sudden developments now threatens the federal judiciary’s autonomy in arguably unprecedented ways and demands a more comprehensive analysis of judicial independence and its vulnerabilities. This article begins by reconceptualizing the structure of judicial independence in three tiers. At the apex is an ancient, Rule of Law Paradigm, which proceeds from the premise …

Proving Discrimination By The Text, Deborah Widiss

Articles by Maurer Faculty

Employment discrimination laws make the “simple but momentous” declaration that it is illegal to deny employment on the basis of race, sex, religion, or other key aspects of identity. But when employees who have been treated unfairly turn to the courts for relief, courts rarely assess whether their claims meet the statutory standard. Instead, they funnel the evidence through a convoluted body of judge-made law known as McDonnell Douglas burden shifting.

This Article lays bare fundamental inconsistencies between the statutes’ causal language, as definitively interpreted by the Supreme Court in recent cases, and the burden-shifting process. In Bostock v. Clayton …

The Architecture Of Judicial Ethics, Charles G. Geyh

Articles by Maurer Faculty

In 1999, Professor Stephen Burbank wrote an article entitled The Architecture of Judicial Independence. It is a foundational piece that gave structure to what was then an understudied field. At the heart of that article is a profound insight: stable and enduring judicial systems are the product of forces in constructive tension. Thus, in the context of judicial administration, Burbank conceptualized judicial independence with reference to judicial accountability, and characterized pressure points in the relationship between them as complementary, not contradictory; and in later work, he made a similar point about the interplay between the law and policy in judicial …

The Perils Of "Old" And "New" In Sentencing Reform, Jessica M. Eaglin

Articles by Maurer Faculty

This Essay turns attention from actuarial risk assessment tools as a reform to the inclination for a technical sentencing reform more broadly. When situated in the context of technical guidelines created to structure and regulate judicial discretion in the 1980s and beyond, the institutionalization of an actuarial risk assessment at sentencing is both an old and new idea. Both sentencing guidelines and actuarial risk assessments raise conceptual and empirical questions about sentencing law and policy. This Essay drills down on two conceptual issues—equality and selective incapacitation—to highlight that actuarial risk assessments as a reform raise recurring questions about sentencing, even …

Esg And Climate Change Blind Spots: Turning The Corner On Sec Disclosure, Cynthia A. Williams, Donna M. Nagy

Articles by Maurer Faculty

This article examines four areas in which the SEC, for more than a decade, resisted reform or impeded shareholders’ access to sought-after environmental, social, and governance (ESG) information. These areas are: (1) the SEC’s refusal to act on several rulemaking petitions submitted during the years 2009 to 2018, which called for expanded ESG disclosure; (2) the SEC’s grudging promulgation of rules concerning social disclosures as required by Congress in the Dodd-Frank Act of 2010; (3) the SEC’s 2020 revisions to SEC Rule 14a-8, which make the submission of shareholder proposals more difficult, thereby thwarting investor efforts to raise ESG concerns; …

Reading Datasets: Strategies For Interpreting The Politics Of Data Signification, Lindsay Poirier

Statistical and Data Sciences: Faculty Publications

All datasets emerge from and are enmeshed in power-laden semiotic systems. While emerging data ethics curriculum is supporting data science students in identifying data biases and their consequences, critical attention to the cultural histories and vested interests animating data semantics is needed to elucidate the assumptions and political commitments on which data rest, along with the externalities they produce. In this article, I introduce three modes of reading that can be engaged when studying datasets—a denotative reading (extrapolating the literal meaning of values in a dataset), a connotative reading (tracing the socio-political provenance of data semantics), and a deconstructive reading …

Improving Microbial Safety Of Food Products By Thermal And Non-Thermal Technology And Evaluate The Knowledge Of Antibiotic Resistant Issue Among Local Produce Growers, Wentao Jiang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Microbial contamination of food products is one of the main transmission routes of disease in the world today, which is responsible for about two-thirds of all food-borne disease outbreaks although the hygiene process was improved recently. Improving microbial safety and implementing a good food management system are important elements to reduce microbial contamination and improve food safety and security. To improve microbial safety, I conducted inactivation studies on food pathogens and further explored antibiotic resistant risks. Initial research evaluated the efficacy of commercial antimicrobials distribution by comparing electrostatic sprayer with conventional sanitization process. The antibiotics applied by electrostatic spraying achieved …

A Role For Endothelial Tissue-Nonspecific Alkaline Phosphatase In Blood-Tissue Barrier Health And Disease, Allison L. Brichacek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sepsis is a disorder that targets the microcirculation with significant effects at blood-tissue barriers such as the blood-brain barrier (BBB) and gut-vascular barrier (GVB). Intestinal barrier dysfunction is thought to be one of the most important contributors to multi-organ dysfunction in sepsis. As a common site of infection, the impaired gut allows the dissemination of bacteria, which exacerbates the disease pathophysiology, and can lead to long-term morbidity and mortality. The mechanisms that influence altered barrier permeability in the GVB in sepsis and other inflammatory disorders are not well understood. Tissue-nonspecific alkaline phosphatase (TNAP) enzyme activity, which has been shown to …

Determining The Effects Of Imidacloprid On Non-Target Soil Organisms In Hemlock Stands, Braley Burke

Graduate Theses, Dissertations, and Problem Reports (ETD)

The hemlock woolly adelgid (HWA), Adelges tsugae Annand (Hemiptera: Adelgidae), is an invasive insect that is causing mortality of eastern hemlock trees, Tsuga canadensis, and Carolina hemlock trees, Tsuga caroliniana, across the eastern United States. To protect these ecologically important tree species, a neonicotinoid insecticide, imidacloprid, is commonly used. Imidacloprid is an effective treatment and can remain effective against HWA for four to six years but long-term (≥ one year after application) non-target effects of imidacloprid on forest ecosystems are not well-studied. This study examined terrestrial non-target effects of imidacloprid in hemlock stands with different treatment histories to …

Janus Kinase 1 Drives Endoplasmic Reticulum Stress-Induced Transcriptional Reprogramming In Astrocytes, Savannah Graham Sims

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neurological and neurodegenerative diseases are heterogenous and devastating diseases with limited therapeutic options and no cures. The broad, long-term goal of this project was to elucidate therapeutic targets for neurodegenerative conditions that attenuate damaging inflammation while leaving the beneficial immune response intact and avoiding broad immunosuppression. Inflammation and the accumulation of misfolded proteins are associated with a wide variety of neurological diseases. Here, we have examined how the accumulation of misfolded proteins shapes inflammatory signaling in the glial cell population astrocytes. Astrocytes are the most populous cell in the central nervous system (CNS) and provide physical and trophic support to …

Remediation Of Spatial Skills In First Semester Calculus Using Haptics-Based Applications, Kristen L. Murphy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Spatial reasoning is required for many topics in undergraduate mathematics as well as other STEM fields. In calculus specifically, there are few interventions that assess students’ spatial skills and provide remediation. Haptic technology is a novel approach to spatial skills remediation due to its unfamiliarity to students and its flexibility with regard to models. The purpose of this dissertation is two-fold: first, to determine what level of spatial abilities students possess upon entering an undergraduate calculus course, and second, to determine whether haptic feedback will enhance an intervention for improving spatial skills.

The dissertation research was a mixed-methods study using …

Terrestrial Salamander And Ant Community Responses To Imidacloprid Application In Central Appalachian Eastern Hemlock Forests, Lenza E. Paul

Graduate Theses, Dissertations, and Problem Reports (ETD)

Imidacloprid is the most widely used insecticide in the world and has been found to impact non-target taxa in systems in which it is applied. It is used as the primary treatment method in the protection of eastern hemlocks (Tsuga canadensis) from impacts of hemlock woolly adelgid (HWA; Adelges tsugae), an invasive insect in the U.S. that is causing widespread mortality of hemlock trees. In this study, we investigated whether imidacloprid applied in eastern hemlock forests is impacting woodland salamander and ant communities.

In Chapter 1, I provide an overview of the ecological importance of eastern hemlocks …

Searching Harder, Localizing Better, Classifying Faster: Optimizing Fast Radio Burst Detection And Analysis, Kshitij Aggarwal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fast Radio Bursts (or FRBs) are millisecond-duration transients of extragalactic origin. They exhibit dispersion caused by propagation through an ionized medium, and quantified by Dispersion Measure (DM). Around 800 FRBs (24 repeaters) have been discovered; so far, 24 FRBs have been confidently associated with a host galaxy. In this thesis, we discuss multiple new FRB search and analysis techniques and the corresponding tools that enable us to search for FRBs harder, localize them better, and classify candidates faster.

We discuss five open-source software suites that can be used in FRB analysis. These suites are used to distinguish between FRBs and …

Response Of Forest Birds To Spotted Wing Drosophila (Drosophila Suzukii Matsumura), A Novel Invasive Fruit Pest, At Allegheny National Forest, Daniel P. Roche

Graduate Theses, Dissertations, and Problem Reports (ETD)

Non-native invasive insect pests can have dramatic impacts on native ecosystems, feeding on plant foliage, wood, or sap. Little is known, however, about how fruit-targeting NNIIPs may affect native ecosystems. Spotted wing Drosophila (Drosophila suzukii Matsumura, SWD) is a recently introduced invasive vinegar fly that parasitizes the fruits of many plant species in the United States. While its activity in agricultural systems is well-documented, little is known about its activity in forest ecosystems, despite growing evidence of its presence and parasitism of fruits there. Parasitism could reduce fruit attractiveness for vertebrate fruit consumers, including migratory birds. As such, this …

The Effect Of Bilingual Proficiency In Indian English On Bilabial Plosive, Taniya Chawla

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Bilingual speech production studies have highlighted that level of proficiency influences the acoustic-phonetic representation of phonemes in both languages (MacKay, Flege, Piske, & Schirru 2001; Zárate-Sández, 2015). The results for bilingual speech production reveal that proficient/early bilinguals produce distinct acoustic properties for the same phoneme in each language, whereas less proficient/late bilinguals produce acoustic properties for a phoneme that is closer to the native language (Flege et al., 2003; Fowler et al., 2008). Acoustic-phonetic studies for Hindi (L1) and Indian English (L2) for bilingual speakers have been understudied, and the level of proficiency has not been considered in Hindi …

Three Essays On Energy And Agricultural Price Analysis, Sara Farhangdoost

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation consists of three essays on energy and agricultural commodity price analysis: 1) Natural Gas Price Forecasting in a Changing World; 2) The Effect of EIA Storage Announcement on Natural Gas Returns: A Comprehensive Analysis; and 3) Forecasting the U.S. Season-Average Farm Price of Corn: Derivation of an Alternative Futures based Forecasting Model.

The first essay evaluates the performances of various individual and composite forecasting models when predicting natural gas prices in the United States. The empirical results show that forecast generated by the Energy Information Administration Short-Term Energy Outlook provides a more accurate price prediction at longer forecasting …