Digital Commons Network^™

Development Of A Near-Infrared Fluorescent Daratumumab-Based Drug Conjugate For Targeted Imaging And Therapy In Preclinical Multiple Myeloma, Nicholas Cho

McKelvey School of Engineering Graduate Student Theses & Dissertations

Multiple myeloma (MM) is a debilitating hematologic malignancy of terminally differentiated plasma cells in the bone marrow (BM). Daratumumab (DARA) is an FDA-approved high-affinity monoclonal antibody targeting CD38 that has shown promising therapeutic efficacy in relapsed and refractory MM. Despite the well-established clinical efficacy of DARA, not all heavily pretreated patients respond to DARA monotherapy, and the majority of patients who initially respond eventually progress. This may be due to upregulation of pathways that inhibit DARA-mediated tumor killing. Antibody drug conjugates (ADCs) combine the highly targeted tumor antigen recognition of antibodies with cell killing properties of chemotherapy for effective internalization …

Metabolic Adaptation To The Chronic Loss Of Ca 2+ Signaling Induced By Ko Of Ip 3 Receptors Or The Mitochondrial Ca 2+ Uniporter, Michael P. Young, Zachary T Schug, David M. Booth, David I Yule, Katsuhiko Mikoshiba, György Hajnóczky, Suresh K Joseph

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Calcium signaling is essential for regulating many biological processes. Endoplasmic reticulum inositol trisphosphate receptors (IP3Rs) and the mitochondrial Ca2+ uniporter (MCU) are key proteins that regulate intracellular Ca2+ concentration. Mitochondrial Ca2+ accumulation activates Ca2+-sensitive dehydrogenases of the tricarboxylic acid (TCA) cycle that maintain the biosynthetic and bioenergetic needs of both normal and cancer cells. However, the interplay between calcium signaling and metabolism is not well understood. In this study, we used human cancer cell lines (HEK293 and HeLa) with stable KOs of all three IP3R isoforms (triple KO [TKO]) or MCU to examine metabolic and bioenergetic responses to the chronic …

Classifying Mosquito Presence And Genera Using Median And Interquartile Values From 26-Filter Wingbeat Acoustic Properties, Hernan S. Alar, Proceso L. Fernandez Jr

Department of Information Systems & Computer Science Faculty Publications

Mosquitoes are known to be one of the deadliest creatures in the world. There have been several studies that aim to identify mosquito presence and species using various techniques. The most common ones involve automatic identification of mosquito species from the sounds produced by flapping its wings. The development of these important concepts and technologies can help reduce the spread of mosquito-borne diseases. This paper presents a simple model based on mean and interquartile values that aim to solve the mosquito classification. Despite its simplicity, the proposed model significantly outperforms a Convolutional Neural Network (CNN) model in identifying the mosquito …

Stabilin Receptors Clear Lps And Control Systemic Inflammation, Fatima Cabral, Mustafa Al-Rahem, John Skaggs, Thushara A. Thomas, Naresh Kumar, Qian Wu, Paolo Fadda, Lianbo Yu, John M. Robinson, Jonghan Kim, Ekta Pandey, Xinghui Sun, Wael N. Jarjour, Murugesan V.S. Rajaram, Edward N. Harris, Latha P. Ganesan

Department of Biochemistry: Faculty Publications

Lipopolysaccharides (LPSs) cause lethal endotoxemia if not rapidly cleared from blood circulation. Liver sinusoidal endothelial cells (LSEC) systemically clear LPS by unknown mechanisms. We discovered that LPS clearance through LSEC involves endocytosis and lysosomal inactivation via Stabilin-1 and 2 (Stab1 and Stab2) but does not involve TLR4. Cytokine production was inversely related to clearance/endocytosis of LPS by LSEC. When exposed to LPS, Stabilin double knockout mice (Stab DK) and Stab1 KO, but not Stab2 KO, showed significantly enhanced systemic inflammatory cytokine production and early death compared with WT mice. Stab1 KO is not significantly different from Stab DK in circulatory …

Bioengineered Models To Study Microenvironmental Regulation Of Glioblastoma Metabolism, Joseph Chen, Hyunchul Lee, Philipp Schmitt, Caleb J. Choy, Donald M. Miller, Brian J. Williams, Elaine L. Bearer, Hermann B. Frieboes

Pathology Research and Scholarship

Despite extensive research and aggressive therapies, glioblastoma (GBM) remains a central nervous system malignancy with poor prognosis. The varied histopathology of GBM suggests a landscape of differing microenvironments and clonal expansions, which may influence metabolism, driving tumor progression. Indeed, GBM metabolic plasticity in response to differing nutrient supply within these microenvironments has emerged as a key driver of aggressiveness. Additionally, emergent biophysical and biochemical interactions in the tumor microenvironment (TME) are offering new perspectives on GBM metabolism. Perivascular and hypoxic niches exert crucial roles in tumor maintenance and progression, facilitating metabolic relationships between stromal and tumor cells. Alterations in extracellular …

Response To Comments For 2020 Bpsou Uao Submittal: Revised Final Multi-Pathway Residential Metals Abatement Program Plan (Received January 21, 2021), Mike Mcanulty

Silver Bow Creek/Butte Area Superfund Site

No abstract provided.

Remote Sensing And Gis Integration For Amazon Rainforest Wildfires Applications, Cong Ma

USF Tampa Graduate Theses and Dissertations

Known as the “Lung of the world”, the Amazon rainforest produces more than 20% of the oxygen of the world, which was originally a carbon pool for mitigating climate change, but in recent years it has become a significant carbon emitter due to wildfires. In 2019, a large-scale fire occurred in the Amazon rainforest, causing serious damage to the ecosystem and to humans as well. Therefore, managing wildfires effectively has become an urgent task for fire authorities. This thesis tried to incorporate spatial analysis and spatial statistics approaches to study wildfire from two aspects, namely the temporal and spatial distribution …

Accumulation Of Legacy Fallout Radionuclides In Cryoconite On Isfallsglaciären (Arctic Sweden) And Their Downstream Spatial Distribution, Caroline C. Clason, Will H. Blake, Nick Selmes, Alex Taylor, Pascal Boeckx, Jessica Kitch, Stephanie C. Mills, Giovanni Baccolo, Geoffrey E. Millward

School of Geography, Earth and Environmental Sciences

Abstract. The release of legacy contaminants such as fallout radionuclides (FRNs) in response to glacier retreat is a process that has received relatively little attention to date, yet may have consequences as a source of secondary contamination as glaciers melt and down-waste in response to a warming climate. The presence of FRNs in glacier-fed catchments is poorly understood in comparison to other contaminants, yet there is now emerging evidence from multiple regions of the global cryosphere for substantially augmented FRN activities in cryoconite. Here we report concentrated FRNs in both cryoconite and proglacial sediments from the Isfallsglaciären catchment in Arctic …

Enhancing Student Learning Of Global Warming Through Reflective Writing, Liang Zeng, Guang Zeng

Physics & Astronomy Faculty Publications

The National Academy of Science has published studies showing strong scientific evidence that global warming is caused by human consumption of fossil fuels, yet recent surveys have shown young adults in the U.S. are disengaged or disagree with this fact. Accordingly, the United Nations Educational, Scientific, and Cultural Organization (UNESCO) published learning objectives to educate the world population on global warming and renewable energy by 2030. In this paper, we introduce a reflective writing activity physics educators can employ to foster a deeper understanding of global warming in introductory college physics and physical science courses, without overloading their teaching time.

Higher Coffee Consumption Is Associated With Slower Cognitive Decline And Less Cerebral Aβ-Amyloid Accumulation Over 126 Months: Data From The Australian Imaging, Biomarkers, And Lifestyle Study, Samantha L. Gardener, Stephanie R. Rainey-Smith, Victor L. Villemagne, Jurgen Fripp, Vincent Doré, Pierrick Bourgeat, Kevin Taddei, Christopher Fowler, Colin L. Masters, Paul Maruff, Christopher C. Rowe, David Ames, Ralph N. Martins, Aibl Investigators

Research outputs 2014 to 2021

Background:

Worldwide, coffee is one of the most popular beverages consumed. Several studies have suggested a protective role of coffee, including reduced risk of Alzheimer’s disease (AD). However, there is limited longitudinal data from cohorts of older adults reporting associations of coffee intake with cognitive decline, in distinct domains, and investigating the neuropathological mechanisms underpinning any such associations.

Methods: The aim of the current study was to investigate the relationship between self-reported habitual coffee intake, and cognitive decline assessed using a comprehensive neuropsychological battery in 227 cognitively normal older adults from the Australian Imaging, Biomarkers, and Lifestyle (AIBL) study, over …

Achievements And Perspectives In The Breeding Of Temperate Grasses And Legumes, Kenneth H. Quesenberry, M. D. Casler

IGC Proceedings (1977-2023)

This paper will focus on a historical perspective on cool season forage production, plant breeding methods for cool season forages, major cool season forage selection criteria, some examples of significant achievements, and a future perspective. Topics similar to ours have been discussed at recent previous meeting of this Congress (Humphreys, 1997; Van Wijk et al., 1993); however, we will strive to avoid “plowing the same ground twice”. In an attempt to prevent duplication of content with other sections of this Congress, only limited attention will be given to genetic resource acquisition and conservation. Additionally, alfalfa (Medicago sativa L.), one …

Achievements And Perspectives In The Breeding Of Tropical Grasses And Legumes, John W. Miles

IGC Proceedings (1977-2023)

Pasture and forage plant breeding is complicated by the perennial nature of the plants, the diversity of environments in which improved cultivars will be used, and the complex criteria of merit involved, criteria that necessarily include some measure of impact on the efficiency of animal production. While pasture plant breeding in the temperate zone is a demonstrably productive activity, the record of success for the tropical species -- "success" measured by release and adoption of bred cultivars -- is less convincing, in spite of four decades of activity in numerous public sector breeding programs and a large published literature. The …

Tropical Forage Genetic Resources -- Will Any Be Left For Future Generations?, B. L. Maass, B. C. Pengelly

IGC Proceedings (1977-2023)

After very active years of pasture and forage research at major institutes, interest in tropical forage genetic resources has drastically declined. Apparently, the early phases of collecting and evaluation were much more valued than conserving and keeping the germplasm available for future generations. Accumulated data are not easily accessible and, therefore, the knowledge of tropical forage genetic resources is progressively being lost. This worldwide decrease in activity and loss of knowledge is due to declining resources. It is suggested that a global database on tropical forage genetic resources should be established and also that finances be made available to at …

Adaptability And Stability Of Alfalfa Cultivars, Ana C. Ruggieri, Alda L. G. Monteiro, R. Gualberto

IGC Proceedings (1977-2023)

Yield adaptability and stability of thirty five alfalfa genotypes (Medicago sativa L.) were evaluated at Sertãozinho, SP, Brazil, considering different environments (seasons of year) from 1996 to 1999. A randomized complete block design with three replications was used. Scott-Knott’s test was performed to compare genotypes averages. Adaptability and stability were determined by Eberhart and Russell's procedures. Two diverse groups of genotypes with mean dry matter yield were identified: Araucana, F686, MH4 e BR2 were more responsive under favorable environments, but SW 8210, Alto, Monarca SP, Victoria SP, Florida 77, P5888, MH15, BR1, BR3, SW 9210A presented broad adaptability and …

Agreement Of A Novel Vertical Jump System To Measure Vertical Jump Height: Brower Vertical Jump And Vertec Vertical Jump Systems, L Nathan Thomas, Parker Hill, Colton Fox, Justin R. Brown

Topics in Exercise Science and Kinesiology

Topics in Exercise Science and Kinesiology Volume 2: Issue 1, Article 12, 2021. Validity refers to the ability of a device to measure what it was intended to measure. Therefore, purpose of this study was to assess the validity and reliability of a novel vertical jump height tool designed by Brower Timing Systems (Salt Lake City, Ut). The Brower vertical jump system was compared to the Vertec jump tester. A convenience sample (n=67) of college students performed three maximum countermovement jumps, with the average score being recorded. Data was collected simultaneously for both devices. Results showed a strong and statistically …

Why Do People Click?! Uses And Gratifications Of Facebook-Zynga Games Among Egyptian Adults, Soha Eissa

Archived Theses and Dissertations

No abstract provided.

A Comparative Study On The Rhetorical Moves Of Abstracts In Published Research Articles And Egyptian Master's Theses In Applied Linguistics, Ahmed Awwaad Ibrahim Awwaad

Archived Theses and Dissertations

No abstract provided.

Humanizing The Colonial Other: The Engaged Reader In Shakespeare, Swift, Conrad, And Barghouti, Rasha Amr Ahmed Malek

Archived Theses and Dissertations

No abstract provided.

Women's Empowerment Made Visual: Digital Storytelling At Pathways Research Consortium, Laura Worcester

Archived Theses and Dissertations

No abstract provided.

Spaces Between: Identities Of Transnational People Expressed Through Their Art And Its Significance, Rebecca Malcolm

Archived Theses and Dissertations

No abstract provided.

Coming-Of-Age And Parallel Worlds In Philip Pullman's His Dark Materials, Reham Rabie

Archived Theses and Dissertations

No abstract provided.

First Person, Colby College, Lokesh Todi

Colby Magazine

No abstract provided.

One Step At A Time: Robotics Lab Team Combines Talents In Quest For The Perfect Synthetic Tendon, Laura Meader

Colby Magazine

When Caitrin Eaton arrived at Colby a year ago, she named her new robotics lab C3PO. The name grabs students’ attention, but they’re drawn to the lab for another reason: Eaton’s cutting-edge research unites engineering and animal physiology to help robots walk more smoothly than Hollywood’s stiff-legged C-3PO robot.

Crash Course: Student Team Uses Statistical Modeling And Bigelow Partnership To Map Moose-Car "Hot Zones", Gerry Boyle, Max Slomiak

Colby Magazine

The project began in 2004 when Alex Jospe ’06, a Nordic skier who traveled Maine roads to meets, decided to use skills learned in a GIS class taught by Associate Professor of Environmental Studies Philip Nyhus. Jospe used data supplied by state transportation officials to map moose-collision hot zones. On a trip to Vermont, the map came in handy. “She came back all excited and said, ‘I saw a moose right where my map said I would,’” Nyhus recalled.

Visual Quality Integerated Cost Model For Developing Green Neighborhoods: An Optimization Apporoach, Eiman Yehia El-Banhawy

Archived Theses and Dissertations

No abstract provided.

Stimulating The Facial Nerve To Treat Ischemic Stroke: A Systematic Review, Turner S. Baker, Justin Robeny, Danna Cruz, Alexis Bruhat, Alfred-Marc Iloreta, Anthony Costa, Thomas James Oxley

Publications and Research

Acute ischemic stroke (AIS) is a common devastating disease that has increased yearly in absolute number of cases since 1990. While mechanical thrombectomy and tissue plasminogen activator (tPA) have proven to be effective treatments, their window-of-efficacy time is very short, leaving many patients with no viable treatment option. Over recent years there has been a growing interest in stimulating the facial nerves or ganglions to treat AIS. Pre-clinical studies have consistently demonstrated an increase in collateral blood flow (CBF) following ganglion stimulation, with positive indications in infarct size and neurological scores. Extensive human trials have focused on trans-oral electrical stimulation …

The Indiana Statesman, November 18, 2021, Indiana State University

Student Newspaper

Feds open civil rights case into Texas school district; Kyle Rittenhouse trial: Jurors continue deliberations; Convictions overturned in Malcolm X '65 case; A thousand dead manatees: Florida surpasses a grim milestone

(Non)Parallel Developmental Mechanisms In Vertebrate Appendage Reduction And Loss, Samantha Swank, Thomas Sanger, Yoel E. Stuart

Biology: Faculty Publications and Other Works

Appendages have been reduced or lost hundreds of times during vertebrate evolution. This phenotypic convergence may be underlain by shared or different molecular mechanisms in distantly related vertebrate clades. To investigate, we reviewed the developmental and evolutionary literature of appendage reduction and loss in more than a dozen vertebrate genera from fish to mammals. We found that appendage reduction and loss was nearly always driven by modified gene expression as opposed to changes in coding sequences. Moreover, expression of the same genes was repeatedly modified across vertebrate taxa. However, the specific mechanisms by which expression was modified were rarely shared. …

Towards A “Filipino” Video Game: Teaching Filipino Culture And Identity For Video Game Development, Christoffer Mitch C. Cerda

Filipino Faculty Publications

This paper uses the author’s experiences of teaching the Filipino module of a multidisciplinary video game development class as a case study in teaching Filipino culture and identity as an element of video game development. A preliminary definition of “Filipino video game” as having Filipino narratives and subject matter, made by Filipino video game developers, and catering to a Filipino audience, is proposed. The realities and limitations of video game development and the video game market in the Philippines is also discussed to show how the dominance of Western video game industry, in terms of the dominance of outsource work …

The Concentration Of Brain Homogenates With The Amicon Ultra Centrifugal Filters, Joshua Yang, Rachita K. Sumbria

Pharmacy Faculty Articles and Research

Accurately measuring the brain concentration of a neurotherapeutic is critical in determining its pharmacokinetic profile in vivo. Biologics are potential therapeutics for neurologic diseases and biologics fused to an antibody targeting a transcytosis receptor at the Blood-Brain Barrier, designated as antibody-biologic fusion proteins, are Blood-Brain Barrier penetrating neurotherapeutics. The use of sandwich immunosorbent assays to measure concentrations of antibody-biologic fusion proteins in brain homogenates has become increasingly popular. The raw brain homogenate contains many proteins and other macromolecules that can cause a matrix effect, potentially interfering with the limit of detection of such assays and reduce the overall sample …