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Cannabidiol In The Horse: Pharmacokinetics And Effects Of A Pelleted Supplement On Reactivity And Movement, Anna L. Draeger

Murray State Theses and Dissertations

A multitude of claims exist regarding therapeutic benefits of cannabidiol (CBD) in human and animal medicine. Though supportive evidence of CBD as a nutraceutical option exists, lack of regulation means that product safety, consistency, and efficacy cannot be guaranteed. Trials for specific conditions and species are needed. The objective of this study was to evaluate CBD safety and use effects on reactivity and movement in the horse. Project 1 examined the bioavailability of a single 50 mg dose of an oil and pelleted CBD product. One of 2 Quarter Horse geldings received the oil product. The second received the pelleted …

Full-Range Space-Division Multiplexing Optical Coherence Tomography Angiography, Yongyang Huang, Jason Jerwick, Guoyan Liu, Chao Zhou

Open Access Publications

In this study, we demonstrated a full-range space-division multiplexing optical coherence tomography (FR-SDM-OCT) system. Utilizing the galvanometer-based phase modulation full-range technique, the total imaging range of FR-SDM-OCT can be extended to >20 mm in tissue, with a digitizer sampling rate of 500 MS/s and a laser sweeping rate of 100 kHz. Complex conjugate terms were suppressed in FR-SDM-OCT images with a measured rejection ratio of up to ∼46 dB at ∼1.4 mm depth and ∼30 dB at ∼19.4 mm depth. The feasibility of FR-SDM-OCT was validated by imaging Scotch tapes and human fingernails. Furthermore, we demonstrated the feasibility of FR-SDM-OCT …

Astrocyte Deletion Of Α2-Na/K Atpase Triggers Episodic Motor Paralysis In Mice Via A Metabolic Pathway, Sarah A. Smith, Xiaoying Chen, Lindsey M. Brier, Jonathan R. Bumstead, Nicholas R. Rensing, Alison E. Ringel, Haewon Shin, Anna Oldenborg, Jan R. Crowley, Annie R. Bice, Krikor Dikranian, Joseph E. Ippolito, Marcia C. Haigis, Thomas Papouin, Guoyan Zhao, Michael Wong, Joseph P. Culver, Azad Bonni

Open Access Publications

No abstract provided.

Harnessing Real-World Data To Inform Decision-Making: Multiple Sclerosis Partners Advancing Technology And Health Solutions (Ms Paths), Ellen M Mowry, Tammie L S Benzinger, Robert T Naismith, Et Al

Open Access Publications

No abstract provided.

A Mobile App Directory Of Occupational Therapists Who Provide Home Modifications: Development And Preliminary Usability Evaluation, An Thi Nguyen, Emily Kling Somerville, Sandra Martina Espín-Tello, Marian Keglovits, Susan Lynn Stark

Open Access Publications

BACKGROUND: Home modifications provided by occupational therapists (OTs) are effective in improving daily activity performance and reducing fall risk among community-dwelling older adults. However, the prevalence of home modification is low. One reason is the lack of a centralized database of OTs who provide home modifications.

OBJECTIVE: This study aimed to develop and test the usability of a mobile app directory of OTs who provide home modifications in the United States.

METHODS: In phase 1, a prototype was developed by identifying OTs who provide home modifications through keyword Web searches. Referral information was confirmed by phone or email. In phase …

Midkine Activation Of Cd8+ T Cells Establishes A Neuron–Immune–Cancer Axis Responsible For Low-Grade Glioma Growth, Xiaofan Guo, Yuan Pan, Min Xiong, Shilpa Sanapala, Corina Anastasaki, Olivia Cobb, Sonika Dahiya, David H. Gutmann

Open Access Publications

No abstract provided.

Human Brain Functional Network Organization Is Disrupted After Whole-Brain Radiation Therapy, Timothy J. Mitchell, Benjamin A. Seitzman, Nicholas Ballard, Steven E. Petersen, Joshua S. Shimony, Eric C. Leuthardt

Open Access Publications

No abstract provided.

Sensitivity Analysis Of Monte Carlo Model Of A Gantry-Mounted Passively Scattered Proton System, Milad Baradaran-Ghahfarokhi, Francisco Reynoso, Michael T Prusator, Baozhou Sun, Tianyu Zhao

Open Access Publications

PURPOSE: This study aimed to present guidance on the correlation between treatment nozzle and proton source parameters, and dose distribution of a passive double scattering compact proton therapy unit, known as Mevion S250.

METHODS: All 24 beam options were modeled using the MCNPX MC code. The calculated physical dose for pristine peak, profiles, and spread out Bragg peak (SOBP) were benchmarked with the measured data. Track-averaged LET (LET

RESULTS: For the physical dose distribution, the MCNPX MC model matched measurements data for all the options to within 2 mm and 2% criterion. The Mevion S250 was found to have a …

Inducible Expression Of Wnt7b Promotes Bone Formation In Aged Mice And Enhances Fracture Healing, Deye Song, Guangxu He, Fangfang Song, Zhepeng Wang, Xiaochen Liu, Lele Liao, Jiangdong Ni, Matthew J. Silva, Fanxin Long

Open Access Publications

No abstract provided.

Heavy Metals Contaminating The Environment Of A Progressive Supranuclear Palsy Cluster Induce Tau Accumulation And Cell Death In Cultured Neurons, Carolina Alquezar, Jessica B. Felix, Elizabeth Mccandlish, Brian T. Buckley, Dominique Caparros-Lefebvre, Celeste M. Karch, Lawrence I. Golbe, Aimee W. Kao

Open Access Publications

No abstract provided.

Polycystin 2 Is Increased In Disease To Protect Against Stress-Induced Cell Death, Allison L. Brill, Tom T. Fischer, Jennifer M. Walters, Arnaud Marlier, Lorenzo R. Sewanan, Parker C. Wilson, Eric K. Johnson, Gilbert Moeckel, Lloyd G. Cantley, Stuart G. Campbell, Jeanne M. Nerbonne, Hee Jung Chung, Marie E. Robert, Barbara E. Ehrlich

Open Access Publications

No abstract provided.

Coronavirus Proteins As Ion Channels: Current And Potential Research, Conor Mcclenaghan, Alex Hanson, Sun-Joo Lee, Colin G Nichols

Open Access Publications

Coronavirus (CoV) outbreaks have recently emerged as a global public health threat due to their exceptional zoonotic potential - a feature arising from their ability to infect a diverse range of potential hosts combined with their high capacity for mutation and recombination. After Severe Acute Respiratory Syndrome (SARS) CoV-1 in 2003 and Middle East Respiratory Syndrome (MERS) CoV in 2012, with the current SARS-CoV-2 pandemic we are now in the midst of the third deadly international CoV outbreak in less than 20 years. Coronavirus outbreaks present a critical threat to global public health and an urgent necessity for therapeutic options. …

Chikungunya Virus Strains From Each Genetic Clade Bind Sulfated Glycosaminoglycans As Attachment Factors, Nicole Mcallister, Michael S. Diamond, Et Al

Open Access Publications

Chikungunya virus (CHIKV) is an arthritogenic alphavirus that causes debilitating musculoskeletal disease. CHIKV displays broad cell, tissue, and species tropism, which may correlate with the attachment factors and entry receptors used by the virus. Cell surface glycosaminoglycans (GAGs) have been identified as CHIKV attachment factors. However, the specific types of GAGs and potentially other glycans to which CHIKV binds and whether there are strain-specific differences in GAG binding are not fully understood. To identify the types of glycans bound by CHIKV, we conducted glycan microarray analyses and discovered that CHIKV preferentially binds GAGs. Microarray results also indicate that sulfate groups …

Antibodies Targeting Epitopes On The Cell-Surface Form Of Ns1 Protect Against Zika Virus Infection During Pregnancy, Alex W. Wessel, Nurgun Kose, Robin G. Bombardi, Vicky Roy, Warangkana Chantima, Juthathip Mongkolsapaya, Melissa A. Edeling, Christopher A. Nelson, Irene Bosch, Galit Alter, Gavin R. Screaton, David H. Fremont, James E. Crowe Jr, Michael S. Diamond

Open Access Publications

No abstract provided.

Tetracycline-Inactivating Enzymes From Environmental, Human Commensal, And Pathogenic Bacteria Cause Broad-Spectrum Tetracycline Resistance, Andrew J. Gasparrini, Jana L. Markley, Hirdesh Kumar, Bin Wang, Luting Fang, Sidra Irum, Chanez T. Symister, Meghan Wallace, Carey-Ann D. Burnham, Saadia Andleeb, Niraj H. Tolia, Timothy A. Wencewicz, Gautam Dantas

Open Access Publications

No abstract provided.

Federated Learning In Medicine: Facilitating Multi‑Institutional Collaborations Without Sharing Patient Data, Micah J. Sheller, Brandon Edwards, G. Anthony Reina, Jason Martin, Sarthak Pati, Aikaterini Kotrotsou, Mikhail Milchenko, Weilin Xu, Daniel Marcus, Rivka R. Colen, Spyridon Bakas

Open Access Publications

No abstract provided.

Focal Dynamic Thermal Imaging For Label‑Free High‑Resolution Characterization Of Materials And Tissue Heterogeneity, Christine M. O'Brien, Hongyu Meng, Leonid Shmuylovich, Julia Carpenter, Praneeth Gogineni, Haini Zhang, Kevin Bishop, Suman B. Mondal, Gail P. Sudlow, Cheryl Bethea, Clyde Bethea, Samuel Achilefu

Open Access Publications

No abstract provided.

Mapping Of The Language Network With Deep Learning, Patrick Luckett, John J Lee, Ki Yun Park, Donna Dierker, Andy G S Daniel, Benjamin A Seitzman, Carl D Hacker, Beau M Ances, Eric C Leuthardt, Abraham Z Snyder, Joshua S Shimony

Open Access Publications

No abstract provided.

Feasibility And Validity Of A Low-Cost Racing Simulator In Driving Assessment After Stroke, Jonathan Tiu, Annie C Harmon, James D Stowe, Amen Zwa, Marc Kinnear, Latch Dimitrov, Tina Nolte, David B Carr

Open Access Publications

There is a myriad of methodologies to assess driving performance after a stroke. These include psychometric tests, driving simulation, questionnaires, and/or road tests. Research-based driving simulators have emerged as a safe, convenient way to assess driving performance after a stroke. Such traditional research simulators are useful in recreating street traffic scenarios, but are often expensive, with limited physics models and graphics rendering. In contrast, racing simulators developed for motorsport professionals and enthusiasts offer high levels of realism, run on consumer-grade hardware, and can provide rich telemetric data. However, most offer limited simulation of traffic scenarios. This pilot study compares the …

Deployment Of Convalescent Plasma For The Prevention And Treatment Of Covid-19, Evan M Bloch, Brenda J Grossman, Jeffrey P Henderson, Et Al

Open Access Publications

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of coronavirus disease 2019 (COVID-19), has spurred a global health crisis. To date, there are no proven options for prophylaxis for those who have been exposed to SARS-CoV-2, nor therapy for those who develop COVID-19. Immune (i.e., "convalescent") plasma refers to plasma that is collected from individuals following resolution of infection and development of antibodies. Passive antibody administration through transfusion of convalescent plasma may offer the only short-term strategy for conferring immediate immunity to susceptible individuals. There are numerous examples in which convalescent plasma has been used successfully as postexposure prophylaxis …

Accuracy Of Electromyometrial Imaging Of Uterine Contractions In Clinical Environment, Hui Wang, Wenjie Wu, Michael Talcott, Robert C Mckinstry, Pamela K Woodard, George A Macones, Alan L Schwartz, Phillip Cuculich, Alison G Cahill, Yong Wang

Open Access Publications

Clinically, uterine contractions are monitored with tocodynamometers or intrauterine pressure catheters. In the research setting, electromyography (EMG), which detects electrical activity of the uterus from a few electrodes on the abdomen, is feasible, can provide more accurate data than these other methods, and may be useful for predicting preterm birth. However, EMG lacks sufficient spatial resolution and coverage to reveal where uterine contractions originate, how they propagate, and whether preterm contractions differ between women who do and do not progress to preterm delivery. To address those limitations, electromyometrial imaging (EMMI) was recently developed and validated to non-invasively assess three-dimensional (3D) …

On Complement, Memory, And Microglia, Robyn S. Klein

Open Access Publications

No abstract provided.

Feasibility And Safety Of Focused Ultrasound-Enabled Liquid Biopsy In The Brain Of A Porcine Model, Christopher Pham Pacia, Lifei Zhu, Yaoheng Yang, Yimei Yue, Arash Nazeri, H. Michael Gach, Michael R. Talcott, Eric C. Leuthardt, Hong Chen

Open Access Publications

No abstract provided.

Deep Learning Assessment Of Breast Terminal Duct Lobular Unit Involution: Towards Automated Prediction Of Breast Cancer Risk, Suzanne C. Wetstein, Ying Liu, Et Al.

Open Access Publications

Terminal duct lobular unit (TDLU) involution is the regression of milk-producing structures in the breast. Women with less TDLU involution are more likely to develop breast cancer. A major bottleneck in studying TDLU involution in large cohort studies is the need for labor-intensive manual assessment of TDLUs. We developed a computational pathology solution to automatically capture TDLU involution measures. Whole slide images (WSIs) of benign breast biopsies were obtained from the Nurses' Health Study. A set of 92 WSIs was annotated for acini, TDLUs and adipose tissue to train deep convolutional neural network (CNN) models for detection of acini, and …

A Functional Role For Eicosanoid-Lysophospholipids In Activating Monocyte Signaling, Gao-Yuan Liu, Sung Ho Moon, Christopher M. Jenkins, Harold F. Sims, Shaoping Guan, Richard W. Gross

Open Access Publications

No abstract provided.

Identification And Characterization Of Novel Trpm1 Autoantibodies From Serum Of Patients With Melanoma-Associated Retinopathy, Juliette Varin, Margaret M. Reynolds, Nassima Bouzidi, Sarah Tick, Juliette Wohlschlegel, Ondine Becquart, Christelle Michiels, Olivier Dereure, Robert M. Duvoisin, Catherine W. Morgans, José-Alain Sahel, Quentin Samaran, Bernard Guillot, José S. Pulido, Isabelle Audo, Christina Zeitz

Open Access Publications

Melanoma-associated retinopathy (MAR) is a rare paraneoplastic retinal disorder usually occurring in the context of metastatic melanoma. Patients present with night blindness, photopsias and a constriction of the visual field. MAR is an auto-immune disorder characterized by the production of autoantibodies targeting retinal proteins, especially autoantibodies reacting to the cation channel TRPM1 produced in melanocytes and ON-bipolar cells. TRPM1 has at least three different isoforms which vary in the N-terminal region of the protein. In this study, we report the case of three new MAR patients presenting different anti-TRPM1 autoantibodies reacting to the three isoforms of TRPM1 with variable binding …

Tictimer Web: Software For Measuring Tic Suppression Remotely, Jonathan K Black, Jonathan M Koller, Kevin J. Black

Open Access Publications

Woods and Himle developed a standardized tic suppression paradigm (TSP) for the experimental setting, to quantify the effects of intentional tic suppression in Tourette syndrome. We previously provided a computer program to facilitate recording tic occurrence and to automate reward delivery during the several experimental conditions of the TSP. The present article describes a web-based program that performs the same functions. Implementing this program on the web allows research sessions to be performed remotely, in tandem with a video calling program. Relevant data for each session, such as the timing of tics and dispensed rewards, are stored in plain text …

Termini Restraining Of Small Membrane Proteins Enables Structure Determination At Near-Atomic Resolution, Shixuan Liu, Shuang Li, Yihu Yang, Weikai Li

Open Access Publications

Small membrane proteins are difficult targets for structural characterization. Here, we stabilize their folding by restraining their amino and carboxyl termini with associable protein entities, exemplified by the two halves of a superfolder GFP. The termini-restrained proteins are functional and show improved stability during overexpression and purification. The reassembled GFP provides a versatile scaffold for membrane protein crystallization, enables diffraction to atomic resolution, and facilitates crystal identification, phase determination, and density modification. This strategy gives rise to 14 new structures of five vertebrate proteins from distinct functional families, bringing a substantial expansion to the structural database of small membrane proteins. …

Fenofibrate Reduces The Severity Of Neuroretinopathy In A Type 2 Model Of Diabetes Without Inducing Peroxisome Proliferator-Activated Receptor Alpha-Dependent Retinal Gene Expression, Jennifer M Enright, Sheng Zhang, Christina Thebeau, Emily Siebert, Alexander Jin, Veda Gadiraju, Xiaodong Zhang, Shiming Chen, Clay F Semenkovich, Rithwick Rajagopal

Open Access Publications

Fenofibrate slows the progression of clinical diabetic retinopathy (DR), but its mechanism of action in the retina remains unclear. Fenofibrate is a known agonist of peroxisome proliferator-activated receptor alpha (PPARα), a transcription factor critical for regulating metabolism, inflammation and oxidative stress. Using a DR mouse model,

Cholesterol 25-Hydroxylase Suppresses Sars-Cov-2 Replication By Blocking Membrane Fusion, Ruochen Zang, James Brett Case, Xiucui Ma, Maria Florencia Gomez Castro, Zhuoming Liu, Qiru Zeng, Haiyan Zhao, Juhee Son, Paul W. Rothlauf, Gaopeng Hou, Michael D. Vahey, Kartik Mani, Daved H. Fremont, Abhinav Diwan, Michael S. Diamond, Sean P.J. Whelan, Siyuan Ding, Et Al.

Open Access Publications

No abstract provided.