Digital Commons Network^™

Valorization Studies On Ice-Cream Wastewater And Whey Permeate, Maryam Enteshari

Electronic Theses and Dissertations

Ever growing demand for dairy products in many countries has led to advancements in the manufacture of such products. Nowadays, revolutionary trends for high-protein dairy foods and drinks products have brought about new challenges from processing requirements and manufacturing aspects. Using different membrane processing has enabled dairy manufacturers to produce different fraction of milk proteins such as micellar caseins, ultra-filtered (UF) milk proteins, whey proteins concentrate (WPC), and micellar casein concentrate (MCC) to meet the nutritional needs with variety of milk product options. However, the installation of new processing lines and establishing further manufacturing steps requires more consumption of water …

Utilizing Rhizospheric And Bacterial Endophytes For Use As Potential Bio-Fertilizers For Sustainable Agricultural Production, Vincent Peta

Electronic Theses and Dissertations

Brassica carinata crop production offers an exciting alternative approach to the energy crisis when looking at novel ways to power our motor vehicles. Bacterial endophytes, while residing in host-plant tissues, offer a wide array of plant growth promotion benefits such as nitrogen fixation to impart nitrogen (N) nutrition, phosphate (P) solubilization to make soil-bound phosphate more mobile in soil for plant uptake, can suppress pathogenic microbes such as fungus and produce plant growth hormones to offset stress incurred by the plant. Previous literature has shown that there is large interest in studying endophytes from economically important crops such as wheat, …

The Status Of Dakota Skipper (Hesperia Dacotae Skinner) In Eastern South Dakota And The Effects Of Land Management, Kendal Annette Davis

Electronic Theses and Dissertations

Hesperia dacotae (Skinner, 1911) (Insecta: Lepidoptera), also known as Dakota skipper, is a northern Great Plains species of butterfly associated with tall or mixed grass prairies. Its range once extended from southern Manitoba, southeastern Saskatchewan, North Dakota, South Dakota, Minnesota, to Iowa and Illinois. Now H. dacotae is only found in small isolated pockets in southern Manitoba, southeastern Saskatchewan, North Dakota, South Dakota, and Minnesota. The species is often observed on alkaline prairies with poor soil not desirable for cultivation. Thus, many of the historical H. dacotae are used for pasturing cattle or as hay prairie. The loss of habitat …

Epitope Mapping And Mechanisms Of Action Of Human Influenza Antibodies Derived From Transchromosomic Cattle, Rongyuan Gao

Electronic Theses and Dissertations

Influenza remains a global health risk and challenge. Currently, NA inhibitors are extensively used to treat influenza, but their efficacy is compromised by the emergence of drug resistant variants. Antibodies targeting influenza A virus surface glycoproteins are critical components of influenza therapeutic agents and may provide alternative strategies to the existing countermeasures. This study characterized both polyclonal and monoclonal antibodies produced from the transchromosomic (Tc) cattle platform. A polyclonal antibody, designated SAB-100, was generated from Tc cattle after immunized with H1N1,H3N2, and influenza B virus. The peptide-based Enzyme-linked immunosorbent assay shows that the polyclonal antibody recognizes three distinct epitopes, one …

Quantitative Imaging Of Cell-Level Auxin Cytokinin Outputs In Soybean And Model Plants, Paul Gaillard

Electronic Theses and Dissertations

Auxin and cytokinin are major plant hormones that orchestrate plant development. These hormones are involved in cell division and differentiation and their spatio‐temporal balance controls organ initiation and maturation. Even though independent transcriptional responses to these hormones have been characterized, their concomitant outputs have not been described. We developed methodology to determine auxin-cytokinin relative output (ACRO) ratios at cellular resolution. Hormone-specific fluorescent markers that localize to the nucleus were used to report transcriptional outputs of auxin and cytokinin. Multiphoton microscopy and image data processing were used to quantify their relative ratio at cellular level in three-dimensional tissue volumes. This strategy …

Epidemiological Investigations Of Bighorn Sheep Respiratory Disease And Implications For Management, Brandi L. Felts

Electronic Theses and Dissertations

Infectious respiratory disease has long been identified as the cause of bighorn sheep (Ovis canadensis) historical declines and extirpations, and Mycoplasma ovipneumoniae (Movi) is the primary pathogen inducing disease and mortality. Population-level effects of pneumonia events range from mild to extirpation. Variable individual response to pathogen exposure emerges from dynamic interactions between competing evolutionary processes within the host and pathogen. Understanding impacts of this evolutionary warfare is essential to assessing long-term impacts of pathogen invasion and developing appropriate countermeasures to protect population health. Freeranging populations are faced with spillover infections from domestic sheep and goats as well as …

Applications Of Hydrodynamic Cavitation In Dairy Manufacturing: Process Development And Standardization, Jae Young Sim

Electronic Theses and Dissertations

The phenomenon of hydrodynamic cavitation involves the formation, growth, and subsequent collapse of bubbles when a given liquid experienced a reduction of pressure below its vapor pressure. The presence of cavitation limits the performance and the safe operation of many machinery and pumps. However, innovation in the design of the hydrodynamic cavitation devices has offered promising applications in the food and dairy industry. Upon collapse of the cavities, the fluid experiences significant mechanical effects (shear and turbulence) as well as instantaneously elevation of the fluid temperature. All these effects can be put to work for mixing, dispersion, particle size reduction, …

Development Of Cellulose Acetate-Based Scaffold For Bone Tissue Engineering Applications, Ruhit Sinha

Electronic Theses and Dissertations

Cellulose Acetate (CA) based biomaterials are being used as substrates for bone ingrowth applications due to their nontoxic and nonirritant nature coupled with optimum morphology and stiffness. Electrospinning with additives and/or post-treatment has emerged as a viable protocol to further improve mechanical properties of CA and expand its utility. Herein, we highlight the role of potassium chloride (KCl) in association with airdrying to enhance the elastic modulus and tensile strengths of CA fibers. Salt aggregation in between fibers is observed through Scanning Electron Microscopy (SEM), however, Fourier-transform infrared (FTIR) analysis signifies the interactions between K+ ions and acetyl groups. The …

Scavenger Hunt: The Search For Sulfur Mustard-Neutralizing Compound In 2-Chloroethyl Ethyl Sulfide (Cees) Treated Human Keratinocyte (Hacat) Epithelial Cells, Tanner Diemer

Electronic Theses and Dissertations

Sulfur mustard is a highly toxic and dangerous vesicant that has been utilized as a chemical warfare agent (CWA) since World War I. Despite its extensive history, an effective antidote to sulfur mustard exposure still does not exist. With detectable levels of unreacted sulfur mustard lasting days after initial contact, a window of opportunity exists to administer a “scavenger” to affected individual immediately and in the days following exposure in hopes of reducing harm by neutralizing unreacted sulfur mustard. For this strategy to be effectively implemented, it is essential to identify a candidate compound with excellent affinity towards sulfur mustard …

Small Rnas: Big Roles In White Mold Infecting Crop Plants, Achal Neupane

Electronic Theses and Dissertations

In eukaryotes, small RNAs (sRNAs) are key regulators of RNA silencing which is also known as RNA interference (RNAi). RNAi is an essential mechanism known in plants, animals and fungi that regulates various biological activities, including defense against foreign nucleic acids and viruses. Fungal pathogens, such as Sclerotinia sclerotiorum, severely limit crop production in all parts of the world. Sclerotinia sclerotiorum cause white mold infection, affecting all dicotyledonous plants including many economically important field crops, vegetables, and floriculture. There does not exist known host resistance, and only limited chemical control can be achieved. Given the growing cost and environmental impacts …

University Assessment Services, National Survey Of Student Engagement: College Of Arts And Sciences, Math And Natural Sciences, First-Year; 2020, Illinois State University

Student Success

National Survey of Student Engagement (NSSE) College of Arts and Sciences, Math and Natural Sciences, Report. Administered by University Assessment Services, Illinois State University, to First-Year students. 2020.

University Assessment Services, National Survey Of Student Engagement: Mennonite College Of Nursing, Seniors; 2020, Illinois State University

Student Success

National Survey of Student Engagement (NSSE) Mennonite College of Nursing Report. Administered by University Assessment Services, Illinois State University, to Seniors. 2020.

Crispr/Cas9 Whole Genome Screens Reveal Novel Regulators Of Endocytic Processes In Macrophages, Jared Wollman

Electronic Theses and Dissertations

Macrophages maintain tissue homeostasis by identifying and eliminating threats within their tissue microenvironment. Pattern recognition receptors allow macrophages to recognize and internalize specific ligands while macropinocytosis allows the internalization of all extracellular solutes from their environment. Without pattern recognition receptors, pathogens may grow unchecked if they cannot be detected, and without macropinocytosis, macrophages struggle to detect and move around their environment. This thesis presents the results of CRISPR/Cas9 whole-genome screens used to identify the regulators of both endocytosis (Chapter 2) and macropinocytosis (Chapter 3). In Chapter 2, we report genes regulating dextran uptake in primary murine macrophages and reveal Mrc1-mediated …

Arthur C. Y. Yao (1906-2004): A Pioneer Chinese Professor At St. Mary's University School Of Law, Robert H. Hu

Faculty Articles

No abstract provided.

Winter 2020, University Of North Dakota Alumni Association

ALL: UND Alumni Review Master List

Winter 2020 issue of "The University of North Dakota Alumni Magazine." 52 pages

Volume 103, Number 4

Remember That Party Last Night? Examining The Effects Of Binge-Like Alcohol On Memory, Anna Thomas

Undergraduate Honors Thesis Collection

Alcohol is a widely available drug that is often abused. Studies have shown alcohol-induced memory loss in humans and animals; however, the large amount of alcohol often necessary to observe such loss and use of involuntary drinking paradigms in animals makes translation difficult. The current study was designed to look at voluntary binge-like drinking behavior and memory in rodents. We anticipated decreases in working memory function following consumption of binge-like alcohol in mice and expected a greater deficit in mice experiencing acute withdrawal during the memory task. The present study explored drinking behaviors in adult C57BL/6J mice (18 male, 18 …

What's In My Histological Dyes Anyway?, Ashley Post

Undergraduate Honors Thesis Collection

There are many different dyes that are used for biological research, but not much is commonly known about the origins of the dyes or how they are synthesized. Our dyes of interest were Coomassie Blue G and Rhodamine B. Coomassie Blue G is a blue colored dye used mainly for SDS-PAGE (gel electrophoresis) in biological research. Rhodamine B is used for its ability to fluoresce under ultraviolet light. Both of these dyes have been used for research purposes for many years despite researchers who use the dyes not having little background knowledge regarding the purity of the manufactured dyes. We …

Exploring The Functionality And Role Of The Interdomain Loop (Idl) Of Mouse Hepatitis Virus (Mhv) Protease Nsp5, Benjamin Nick

Undergraduate Honors Thesis Collection

Human coronaviruses are responsible for a number of physiological ailments and include illnesses such as the common cold, Middle East Respiratory Syndrome (MERS), Severe Acute Respiratory Syndrome (SARS), pneumonia, and COVID-19. While vaccines exist for a multitude of pathogenic viruses that exists today, human coronaviruses are not among those that possess a stable and efficacious vaccine available on the market. Attempts to make an inhibitor against coronaviruses have targeted the active site and substrate binding sites of a protease known as non-structural protein 5 (Nsp5), however these attempts have failed to date. Models of the structure of MHV Nsp5 show …

Terrible Am I, Child?, Camille Arnett

Undergraduate Honors Thesis Collection

The modern period of intergenerational strife between the aging-out Baby Boomers and the Millennials who have come forth to replace them in an infrastructure that cannot support them is a struggle that carries with it unique psychological implications ripe for literary exploration. Understanding these conflicts in a profound way is an important challenge to take on, and one which can, in my belief, be best achieved through literature. My work, a draft of a novel entitled Terrible Am I, Child?, is a family drama which takes the symbolic generational divide and uses it as a framework for exploring issues of …

Paternalism And Autonomy In Transgender Healthcare, Charles Finley

Undergraduate Honors Thesis Collection

Transgender healthcare has made considerable advancements in the quality of patient care and gender confirmation surgical technique throughout the past several decades, which has led to a significant increase in patient satisfaction and overall physical and mental wellbeing. However, there are still several issues that remain to be addressed, many associated with excess paternalism by physicians and violations of patient autonomy. These problems can be mitigated by improving LGBTQ+ and transgender-related medical education for physicians, updating healthcare insurance coverage requirements to be more in line with current care standards, developing appropriate guidelines for lifelong primary care and geriatric care, and …

The Role Of Gender In The Perception Of Lipreading, Emma Richards

Undergraduate Honors Thesis Collection

The experiment examines the gender differences in lipreading ability. We tested 25 fluent English-speaking adults with normal hearing and vision. Each participant was asked to watch four videos testing their lipreading ability with matched and nonmatched gendered speakers. The participants were asked to transcribe 40 sentences, which were scored on the number of target words correct. It was found that participants who watch the videos with matched genders perceived more target words than in the mismatched gender videos. The results could explain previous inconsistencies in earlier research of whether ability to lipread is improved by the gender of the speaker.

Characterization Of Ph-Based Inactivation Of Respiratory Syncytial Virus (Rsv), Jenna Nosek

Undergraduate Honors Thesis Collection

Respiratory syncytial virus (RSV) is a negative-sense, single-strand RNA virus that affects the upper and lower respiratory system in humans. Currently, RSV is the leading cause of infant mortality worldwide, while also infecting target populations of the elderly and immunocompromised. Significant efforts towards vaccine development have been made due to limited therapeutic options, however, physical instabilities of RSV may hinder movement in vaccine development. We hypothesize that strain-specific differences in stability likely attribute to differences in RSV F protein, as proposed by previous studies about the intrinsically instability of RSV. A panel of recombinant RSV viruses were analyzed for strain-specific …

The Effect Of Natural Extracts On Border Cell And Centripetal Cell Migration In The Developing Drosophila Melanogaster Egg Chamber, Kirandeep Kaur

Undergraduate Honors Thesis Collection

Cancer is one of the leading causes of death in the world. Although targeted therapies that specifically inhibit pathways that are activated in cancer cells are becoming more common, often times the specific cancer-causing pathways are not known, or a treatment targeting that pathway has not been developed. In the absence of a targeted therapy, most common cancer treatments target proliferating cells, which can cause many unwanted adverse side effects. Therefore, researchers are testing whether natural extracts or dietary supplements could reduce the growth or metastasis of cancer cells without as many negative side effects. This study uses the Drosophila …

Language-Deprived Environments: Neonatal Intensive Care Units And Hearing Loss, Margaret Caesar

Undergraduate Honors Thesis Collection

Adequate language exposure is crucial for speech-language development in children. Previous research suggests that decreased language exposure leads to an increased risk for speech-language developmental delays. This study aims to explore the language exposure of two arguably language-deprived environments: Neonatal Intensive Care Units (NICU) and severe-to-profound hearing loss. This is a two-part study that examines caregiver-infant interactions through the analysis of infant-directed speech (IDS). In the first part of the study, researchers transcribed and analyzed pre-recorded Language Environment Analysis (LENA) audio recordings of five (5) infants with severe-to-profound hearing loss (6-months post cochlear implantation). These LENA recordings collected speech, language, …

Characterization Of Heavy Metal Bioremediation Pathways In Local Moss Species, Zachary Hanquier

Undergraduate Honors Thesis Collection

Heavy metals are found in natural water sources and are toxic to plants and animals. Metal removal remains a goal of environmental protection; one promising approach is to use plants that have been genetically modified to better remove metals from water. A known metabolic pathway to neutralize metals uses the Phytochelatin Synthase (PCS) enzyme to catalyze the production of phytochelatins. Our research characterizes the potential of Indiana moss species for heavy metal bioremediation as foundational research for future genetic modification. Mosses grow well in water and absorb metals through their entire surface area, and using locally collected moss species will …

The Characterization And Reactivity Of Tris(4-Methylpyrazolyl)Methane And Its Tungsten And Molybdenum Metal Complexes, Julia M. Pomeroy

Undergraduate Honors Thesis Collection

The scorpionate ligand tris(pyrazolyl)borate (Tp) has contributed to the understanding of coordination and organometallic chemistry and has proven to be useful in the synthesis of metalloprotein models. Studies focusing on the similar ligand, tris(pyrazolyl)methane (Tpm), have recently been made possible with improved synthetic techniques. Characterization of the Tpm ligand and its metal derivatives, [TpmW(CO)3L]+ and [TpmMo(CO)3L]+, unveiled unexpected activity in the 1H NMR chemical shift of the methane hydrogen after the coordination of a seventh ligand. The more electronegative the seventh ligand was, the more downfield the chemical shift appeared. Various analogs of Tpm have since been synthesized that substitute …

The Little Orange Book: Short Lessons In Excellent Teaching, The University Of Texas System Academy Of Distinguished Teachers, Art Brownlow, Beth Brunk-Chavez, Weston Rose

School of Music Faculty Publications

The Little Orange Book captures reflections, tips and commentaries on teaching and learning from the members of the University of Texas System Academy of Distinguished Teachers. Its many vignettes span a wide range of topics and teaching interests, from establishing a safe learning space to classroom silences, from curriculum development to modeling the best teachers, and from giving thanks to those teachers who came before us to leaving our own legacies. The Little Orange Book is the perfect text for first-time college instructors who are just getting started on their instructional careers, as well as longtime faculty who have many …

2020-2021 Common Data, Morehead State University. Institutional Research & Analysis.

Office of Institutional Research & Analysis Publication Archive

2020-2021 issue of the Common Data.

Cognitively Enhanced Humans As Both Warfighters And Weapons Of War, Carolyn Sharp

University of Florida Journal of Law & Public Policy

With the advent of neuromorphic computing, artificial intelligence and machine learning can now behave in a human-like manner by analyzing information and responding with subjective reasoning. And when this informed decision-making process is combined with brain-computer interfaces (BCIs), not only could a neuromorphic BCI take direction from the brain, but it could also observe its complexity and use that information to incorporate new knowledge and capabilities into the brain itself as it exploits the plasticity, or adaptability, of the brain. This combination of biological and technological intelligence could lead to the development and delivery of thoughts/outputs that are powerful enough …

Patterns Of Epiphytic Lichen Abundance On Aspen Stands In Proximity To Roads Of Varying Vehicular Traffic, D. Aragon, C. Castillo, J. Moffroid, G. Thomas

Aspen Bibliography

Although present in nearly any wild space with available moisture and on a wide variety of substrates, lichen, and its importance as a bioindicator for an ecosystem, is often overlooked. As air pollution becomes a greater concern for flora, fauna, and even humans, the story told by lichen growth becomes more useful as we try to make sense of the downstream effects of anthropogenic contributions to poor air quality. One such human-driven pollutant is the level of emissions that result from vehicular travel. The Jackson Hole area has experienced a large increase in vehicular traffic in the past five to …