Digital Commons Network^™

Plant Microbial Interactions In Wheat: Fusarium Head Blight And Arbuscular Mycorrhizal Fungi, Yaqoob Rashid Thurston

Electronic Theses and Dissertations

Plant microbial interactions consist of the many relationships between plants and microbes which involve studies that observe the biology and molecular genetics of pathological, symbiotic, and associative interactions. Worldwide studies involving these interactions are scarcely available in wheat (Triticum aestivum L.). In South Dakota (SD), wheat research is a major platform used to understand the nature and consequences of these interactions. Specifically, our research efforts here at South Dakota State University (SDSU) concentrate on two different, but valuable, interactions in wheat: the fungal pathogen that causes fusarium head blight (FHB) and the symbiotic interaction of arbuscular mycorrhizae fungi (AMF) with …

Analysis Of Coevolving Residues Of Xcdxcdx-Phd, A Distinct Type Of Phd-Finger, Shraddha Basu

Electronic Theses and Dissertations

Plant homeodomain (PHD), a Zinc-finger scaffold, is a conserved protein module in eukaryotes that typically recognizes unstructured histone tails, and thus, PHDs play a crucial role in chromatin signaling. The sequences of Zinc-fingers, in general, diversify during the course of evolution often giving rise to subtypes (e.g., RBR-RING (Dove 2017) or zf-CxxC subtypes (Long 2013) who typically contain specific sequence signatures. We recently discovered that PHD fingers also contain a distinct subtype, namely the xCDxCDx-PHD. xCDxCDx-PHD has a distinct composition of specific amino acids that coevolved (coevolving residues) in the course of evolution, and xCDxCDx-PHD also shows a unique mechanism …

Feeding Increasing Levels Of Reduced-Oil Distillers Dried Grains With Solubles From Two Ethanol Fermentation Methods Impacts Finishing Pig Growth Performance And Belly Quality, Austin Egolf

Electronic Theses and Dissertations

Reduced-oil distillers dried grains with solubles (DDGS) are an affordable source of energy in finishing pig diets, but differences in DDGS fermentation method on finishing pig growth performance and belly quality is relatively unknown. The objectives of this study were: 1) determine the influence of increasing dietary inclusion rate of hot fermentation (HF) and cold fermentation (CF) DDGS on growth performance, carcass characteristics and belly quality, and 2) compare the effect of HF and CF DDGS on finishing pig growth performance, carcass characteristics, and belly quality. Crossbred pigs (n = 200) were used in a randomized complete block design and …

Solvolytic Lignocellulosic Biomass Liquefaction, Mustafa Alluhaibi

Electronic Theses and Dissertations

Increasing energy demand, petroleum prices, global warming, and depleting fossil fuel resources are the main challenges faced by the human beings. Many scientists are searching for sustainable and alternative sources of fossil fuels as solutions to these challenges. Lignocellulosic biomass is one of the renewable and eco-friendly abundant sources that has been considered by both academia and industry sectors as a renewable source for bio-oil and chemicals. Direct liquefaction of biomass in the sub-/super-critical solvent has considered a practical method to convert lignocellulosic material into liquid fuel. However, undesirable properties such as poor stability, low energy value, and high acidity …

Evaluation Of Processing Variables To Increase The Solids Of Milk Protein Concentrates Prior To Drying, Achyut Mishra

Electronic Theses and Dissertations

It is a common practice in dairy industries to nanofilter milk protein concentrate (MPC) to increase the solids and further drying efficiency. Increasing protein and solids in MPC increases viscosity and resist feed-flow in membrane filtration. Previous research proved that increased temperature and hydrodynamic cavitation (HC) have significantly reduced the viscosity of MPC which can potentially increase the level of solids by reducing concentration polarization during nanofiltration (NF). We utilized plate and frame filtration (PF) as an alternate method to reduce the viscosity of MPC. In first objective, we studied the application of HC or elevated temperature (50˚C) or both …

Characterization Of Naturally Derived Polymer By Oxidative, Thermal, And Spectrometric Methods, Ranen Roy

Electronic Theses and Dissertations

Extensive use of fossil fuel for different purposes has resulted in significant depletion of it at an alarming rate. Therefore, a potential and sustainable alternative source are badly needed at this moment. Nowadays, lignocellulose biomass is appealing to much interest by the researchers because of its potential nature as a renewable carbon source. It is the most abundant renewable source which is composed of cellulose, hemicellulose, and lignin. Among them, lignin is considered the 2nd most abundantly natural polymer after cellulose which comprises 10-30 % of biomass depending on the source and the environment. Lignin is a three-dimensional cross-linked organic …

Bedding Application And Increasing Dosage Of Trenbolone Acetate And Estradiol In Implants For Beef Steers: Influence On Growth Performance, Carcass Characteristics, And Circulating Metabolite Responses, Dathan T. Smerchek

Electronic Theses and Dissertations

Three randomized complete block design feedlot experiments were conducted over the course of two years. Two experiments were conducted to investigate the effect of bedding use in confined beef steers. The third experiment evaluated the effects of implants containing increasing doses of trenbolone acetate (TBA) and estradiol benzoate (EB) in confined beef steers. Experiment 1 used Simmental × Angus steers (n = 240; initial body weight (BW) = 365 ± 22.5 kg). Experiment 2 used newly weaned Charolais × Red Angus steers (n = 162; initial BW = 278 ± 13.4 kg). Steers were allotted to 1 of 2 treatments: …

Evolving Into The Open: A Framework For Collaborative Design Of Renewable Assignments, Stacy Katz, Jennifer Van Allen

Publications and Research

Open pedagogy reconceives the notion of who creates knowledge and provides a pathway to empower students as creators. Leveraging open education resources in the classroom results in numerous benefits for students, including free access to knowledge, a participatory culture, and opportunities for innovation and creativity (Hegarty, 2015). Open pedagogical design often results in renewable course assignments, which empower students to create authentic resources that can be positioned to have greater impact through time, space, and gravity, particularly as they have longevity, reach, and value (Seraphin et al., 2018). In this chapter, we will highlight a collaborative partnership between library and …

Participatory Action Research In Schools: Unpacking The Lived Inequities Of High Stakes Testing, Tiffany A. Dejaynes, Tabatha Cortes, Israt Hoque

Publications and Research

Purpose – This paper aims to examine a school-based Youth Participatory Action Research (YPAR) project on educational inequity and high stakes testing.

Design/methodology/approach – A former high school teacher (currently a university professor) and two former students (currently research assistants and university students) take up a youth studies framework to collaboratively resee multimodal artifacts from a tenth-grade course in qualitative research.

Findings – Findings illustrate the power of finding allies in peers and educators; the transformative power of deep participation; and the longitudinal nature of social change and action. Thus, this research demonstrates that when students are positioned as researchers, …

Do The Anatomical And Physiological Properties Of A Muscle Determine Its Adaptive Response To Different Loading Protocols?, Brad J. Schoenfeld, Andrew D. Vigotsky, Jozo Grgic, Cody Haun, Bret Contreras, Kenneth Delcastillo, Aston Francis, Gilda Cote, Andrew Alto

Publications and Research

It has been proposed that superior muscle hypertrophy may be obtained by training muscles predominant in type I fibers with lighter loads and those predominant in type II fibers with heavier loads.

Purpose: To evaluate longitudinal changes in muscle strength and hypertrophy of the soleus (a predominantly slow-twitch muscle) and gastrocnemius (muscle with a similar composition of slow and fast-twitch fibers) when subjected to light (20–30 repetition maximum) and heavy (6–10 repetition maximum) load plantarflexion exercise.

Methods: The study employed a within-subject design whereby 26 untrained young men had their lower limbs randomized to perform plantarflexion with a low-load (LIGHT) …

Governance Of Arctic Shipping: Rethinking Risks, Human Impacts And Regulation, Aldo Chircop, Floris Goerlandt, Claudio Aporta, Ronald Pelot

OER Texts

This open access book is a result of the Dalhousie-led research project Safe Navigation and Environment Protection, supported by a grant from the Ocean Frontier Institute’s the Canada First Research Excellent Fund (CFREF). The book focuses on Arctic shipping and investigates how ocean change and anthropogenic impacts affect our understanding of risk, policy, management and regulation for safe navigation, environment protection, conflict management between ocean uses, and protection of Indigenous peoples’ interests. A rapidly changing Arctic as a result of climate change and ice loss is rendering the North more accessible, providing new opportunities while producing impacts on the Arctic. …

The Boy In The Bar Of Soap, Delaney Novak

The Laureate

No abstract provided.

A Cathedral Not Made By Hands, Vincent J. Miller

Theology and Religious Studies Faculty Publications

In Laudato si’, Pope Francis offers a vision of moral responsibility rooted in awareness of the world around us. He points to St. Francis, who “looked with love” on all creatures, as a model. He writes of an “attitude of the heart, one which approaches life with serene attentiveness, which is capable of being fully present” to everyone and everything. And he also calls for an “intense dialogue” between religion and science, which has its own “gaze.” The H.J. Andrews Experimental Forest in Oregon, one of the world’s most studied ecosystems, offers an especially rich opportunity for such dialogue. Here …

The Parmesan Cheese Model System: Alleviation Of Browning Defect In Parmesan Cheese, Megan Huegli Aubrey

Electronic Theses and Dissertations

In cold temperature storage, Parmesan cheese can develop an undesirable browning defect. Certain pyrazine compounds converted from methylglyoxal have been identified and associated with the brown pigmentations in Parmesan cheese samples. This study focused on creating a cheese model system to study variables that caused browning and variables that helped reduce or alleviate browning. The cheese making steps for the cheese model system were monitored by measuring pH and TA and the final composition for % fat, % moisture, % salt and pH. Additionally, the cooling curve was studied to ensure all wheels of cheese made were cooled similarly. Cheese …

Characterization Of Ovine Ileal Epithelial And Myofibroblast Cell Lines And Their Role In Innate Immunity, Shaurav Bhattarai

Electronic Theses and Dissertations

Development of continuous cell lines has been an indispensable tool for studying disease pathogenesis at cellular and molecular level. Intestinal epithelial cells serve as a barrier between the luminal contents and internal tissue and face unique challenge in maintaining homeostasis. Any form of disruption in the uppermost intestinal epithelium exposes underlying intestinal subepithelial myofibroblast (ISEMF) cells to the luminal content, which results in the generation of immune responses from ISEMF cells. Our main goal was to establish and characterize intestinal epithelial and myofibroblast cell lines from a 3-day old sheep. ISEMF cells play significantly important role in wound healing, tissue …

Analysis Of Two Maize Mutations That Arose After Exposure To Radiation From A Plutonium-Beryllium Source And Developing Molecular Markers For Antimorph Aei1-5180, An Alternative To Aei To Increase Amylose Content In Maize Endosperm, Prameela Awale

Electronic Theses and Dissertations

This thesis consists of two independent projects dealing with two different aspects of maize. The first project is about the maize reproduction, where we try to uncover the candidate genes of two mutations that affect pollen viability and successful fertilization. The second project is about maize endosperm starch, where we developed molecular markers to characterize a mutant allele that knocks a major starch branching enzyme in maize.

Analysis of two maize mutations that arose after exposure to radiation from a plutonium-beryllium source
Maize is a monoecious and diclinous plant. Sexual reproduction takes place by the fusion of haploid male and …

Soil Physical And Hydrological Properties, And Greenhouse Gas Emissions Under Integrated Crop-Livestock Agroecosystems, Navdeep Singh

Electronic Theses and Dissertations

Cover crops (CCs) and grazing play a critical role in successful implementation of the integrated crop-livestock system (ICLS) because they can have a direct impact on soils and greenhouse gas emissions. The objectives of this study were to (i) evaluate the short-term impacts of CCs [grass dominated cover crops (GdC) and legume dominated cover crops (LdC)] and grazed CCs and corn (Zea mays L.) residue under oat (Avena sativa L.)–CC–corn rotation on soil physical and hydrological properties; (ii) quantify the architecture of soil pores using X-ray computed tomography (CT) for soils managed under long-term ICLS, native grazed pasture and corn-soybean …

Cover Crop Nutrient Cycling On Western South Dakota Croplands, Justin Brown

Electronic Theses and Dissertations

Cover crops (CC) are widely gaining attention and implementation by producers. Much of this is due to the positive influence that CCs can have. Increases in crop yield, biological diversity, water infiltration, and nutrient cycling have been observed as well as reductions in wind and water erosion. The semiarid climate of western South Dakota presents a challenge in the use of CCs with the limited rainfall received. Little research has been done in this area on the effects of CCs on cropland. Three studies were performed to evaluate CCs on nutrient cycling, forage production and crop yield. First, is an …

Tailoring The Grain Boundaries Of Wide-Bandgap Perovskite Solar Cells By Molecular Engineering, Khalid Emshadi

Electronic Theses and Dissertations

Due to the attraction of fabricating highly efficient tandem solar cells, wide-bandgap perovskite solar cells have attracted substantial interest in recent years. However, polycrystalline perovskite thin-films show the existence of trap states at grain boundaries, which diminish the optoelectronic properties of the perovskite and thus remains a challenge. This research demonstrates a one-step solution-processing of the [MA_0.9Cs_0.1Pb(I_0.6Br_0.4)3] wide-bandgap perovskite using Phenylhydrazine Iodide with amino groups to successfully passivate the trap density within grain boundaries and increase the perovskite grain size. The reinforced morphology and grain boundaries treatment considerably enhanced the photovoltaic performance …

Enhanced Beta (Β) Glucan Beverages – Evaluation Of Satiety, Nutrition, And Shelf Stability, Beatrice Serwaa Manu

Electronic Theses and Dissertations

Oats and barley are unique cereals owing to their nutritional and health benefits. Soluble dietary fiber components, especially β-glucan found in both grains, have been associated with the reduction of cholesterol, normalization of blood sugar levels and suppression of hunger. These nutritional and therapeutic attributes are ideal for production of novel and more diversified food products from these cereals. β-glucan, however, imparts high viscosity in food systems particularly in high moisture foods, thus impairing their sensory characteristics. Reduced viscosity oat-barley beverages, using partial enzyme and acid hydrolysis, may fill a void in the market segment and yet serve as effective …

Developing And Evaluating Novel Methods To Estimate Bread Baking Quality Of Winter And Spring Wheat Cultivars, Bipin Rajpurohit

Electronic Theses and Dissertations

Bread loaf volume is a significant economic factor in the baking industry. Numerous flour and dough quality tests exist in estimating the functional traits of wheat. However, none of these tests accurately or fully predict bread loaf volume. The ultimate reliable method to determine the baking potential of wheat flours is a standard baking experiment which is time-consuming and effort-intensive. Therefore, there is a need to develop empirical methods to predict the baking potential of wheat flours that use small sample sizes and yet, are economical to implement in large throughput wheat breeding programs. A Vacuum Dough Expansion System (VDES) …

Antidiabetic Activity Of Cissus Rotundifolia Plant Growing In Saudi Arabia, Saad Ali Alshehri

Electronic Theses and Dissertations

Diabetes mellitus (DM) is a metabolic disease characterized by high levels of blood glucose resulting from defects in insulin production or action. A world-wide increase in the diabetes rate is projected to reach pandemic levels over the next 10-20 years. Current data shows that high numbers of the population in Saudi Arabia suffer from diabetes and prediabetes. Non-traditional treatments of diabetes from medicinal plants has shown potential antidiabetic activity. A considerable percentage of the population still prefer or use medicinal plants as a treatment or supplement to traditional medicine. Cissus rotundifolia (family Vitaceae) is a known plant in southwestern Saudi …

Effect Of Soy Lecithin Concentration On Formulating Dairy Emulsions Through Ultrasound Treatment, Collette Kernyuy Nyuydze

Electronic Theses and Dissertations

The ability of high intensity ultrasound to produce stable emulsions without the addition of surfactant was evaluated in a dairy-based formulation. The formulation consisted of protein (4.33 ± 0.05%, whey protein concentrates (WPC80), carbohydrates (21.52 ± 0.75%, sucrose and maltodextrin), oil (2.90 ± 0.05%, soybean oil), and surfactant (0-.05%). Pre-emulsions formulated with either 0, 0.025, and 0.05% of soy lecithin were treated for 5 min at an acoustic intensity of either 42.58 ± 2.98, 56.83 ± 3.01, or 70.48 ± 2.97 W cm^-2. The stability of the emulsions was evaluated through particle size, dynamic rheology, gel electrophoresis, and …

Implementing And Assessing The Use Of A New Strategy For Training Chemistry Graduate Teaching Assistants, Amanda Hyett

Electronic Theses and Dissertations

Graduate teaching assistants (GTAs) largely contribute to undergraduate education but are often underprepared for their role as educators. Most graduate students attend graduate school to perform research and are then asked to teach for the first time without sufficient pedagogy training. To assist in increasing the GTAs pedagogical knowledge, a scenario-based activity series was developed and implemented for first- and second-year GTAs. Developing scenario-based scenarios from actual laboratory events provided GTAs with the opportunity to practice prior to engaging in the laboratory classroom. The Teaching Assistant Intervention Activities (TAIAs) included topics such as interpersonal skills and behaviors, group process, working …

Valorization Studies On Ice-Cream Wastewater And Whey Permeate, Maryam Enteshari

Electronic Theses and Dissertations

Ever growing demand for dairy products in many countries has led to advancements in the manufacture of such products. Nowadays, revolutionary trends for high-protein dairy foods and drinks products have brought about new challenges from processing requirements and manufacturing aspects. Using different membrane processing has enabled dairy manufacturers to produce different fraction of milk proteins such as micellar caseins, ultra-filtered (UF) milk proteins, whey proteins concentrate (WPC), and micellar casein concentrate (MCC) to meet the nutritional needs with variety of milk product options. However, the installation of new processing lines and establishing further manufacturing steps requires more consumption of water …

Utilizing Rhizospheric And Bacterial Endophytes For Use As Potential Bio-Fertilizers For Sustainable Agricultural Production, Vincent Peta

Electronic Theses and Dissertations

Brassica carinata crop production offers an exciting alternative approach to the energy crisis when looking at novel ways to power our motor vehicles. Bacterial endophytes, while residing in host-plant tissues, offer a wide array of plant growth promotion benefits such as nitrogen fixation to impart nitrogen (N) nutrition, phosphate (P) solubilization to make soil-bound phosphate more mobile in soil for plant uptake, can suppress pathogenic microbes such as fungus and produce plant growth hormones to offset stress incurred by the plant. Previous literature has shown that there is large interest in studying endophytes from economically important crops such as wheat, …

The Status Of Dakota Skipper (Hesperia Dacotae Skinner) In Eastern South Dakota And The Effects Of Land Management, Kendal Annette Davis

Electronic Theses and Dissertations

Hesperia dacotae (Skinner, 1911) (Insecta: Lepidoptera), also known as Dakota skipper, is a northern Great Plains species of butterfly associated with tall or mixed grass prairies. Its range once extended from southern Manitoba, southeastern Saskatchewan, North Dakota, South Dakota, Minnesota, to Iowa and Illinois. Now H. dacotae is only found in small isolated pockets in southern Manitoba, southeastern Saskatchewan, North Dakota, South Dakota, and Minnesota. The species is often observed on alkaline prairies with poor soil not desirable for cultivation. Thus, many of the historical H. dacotae are used for pasturing cattle or as hay prairie. The loss of habitat …

Epitope Mapping And Mechanisms Of Action Of Human Influenza Antibodies Derived From Transchromosomic Cattle, Rongyuan Gao

Electronic Theses and Dissertations

Influenza remains a global health risk and challenge. Currently, NA inhibitors are extensively used to treat influenza, but their efficacy is compromised by the emergence of drug resistant variants. Antibodies targeting influenza A virus surface glycoproteins are critical components of influenza therapeutic agents and may provide alternative strategies to the existing countermeasures. This study characterized both polyclonal and monoclonal antibodies produced from the transchromosomic (Tc) cattle platform. A polyclonal antibody, designated SAB-100, was generated from Tc cattle after immunized with H1N1,H3N2, and influenza B virus. The peptide-based Enzyme-linked immunosorbent assay shows that the polyclonal antibody recognizes three distinct epitopes, one …

Quantitative Imaging Of Cell-Level Auxin Cytokinin Outputs In Soybean And Model Plants, Paul Gaillard

Electronic Theses and Dissertations

Auxin and cytokinin are major plant hormones that orchestrate plant development. These hormones are involved in cell division and differentiation and their spatio‐temporal balance controls organ initiation and maturation. Even though independent transcriptional responses to these hormones have been characterized, their concomitant outputs have not been described. We developed methodology to determine auxin-cytokinin relative output (ACRO) ratios at cellular resolution. Hormone-specific fluorescent markers that localize to the nucleus were used to report transcriptional outputs of auxin and cytokinin. Multiphoton microscopy and image data processing were used to quantify their relative ratio at cellular level in three-dimensional tissue volumes. This strategy …

Epidemiological Investigations Of Bighorn Sheep Respiratory Disease And Implications For Management, Brandi L. Felts

Electronic Theses and Dissertations

Infectious respiratory disease has long been identified as the cause of bighorn sheep (Ovis canadensis) historical declines and extirpations, and Mycoplasma ovipneumoniae (Movi) is the primary pathogen inducing disease and mortality. Population-level effects of pneumonia events range from mild to extirpation. Variable individual response to pathogen exposure emerges from dynamic interactions between competing evolutionary processes within the host and pathogen. Understanding impacts of this evolutionary warfare is essential to assessing long-term impacts of pathogen invasion and developing appropriate countermeasures to protect population health. Freeranging populations are faced with spillover infections from domestic sheep and goats as well as …