Digital Commons Network^™

Manipulation Of Genome Integrity Signaling Axes Contributing To Prostate Cancer Aggressiveness, Frank Duan, Veronica Rodriguez-Bravo, Phd

Phase 1

Introduction: Prostate cancer (PC) is one of the leading causes of death in men, and despite current treatment options patients can develop aggressive disease that spreads to other parts of the body. Little research has focused to date on the effect of centrosome biology in the pathogenesis of PC.

Methods: Two metastatic PC cell lines, DU145 and 22Rv1, as well as highly aggressive derivatives generated in Dr. Rodriguez-Bravo lab (docetaxel-resistant; (DR) variants) were compared through various experiments to determine differences in centrosome proteins expression and functionality. All cells (DU145 /DR and 22Rv1 /DR) underwent electroporations with siRNAs to knockout …

Race And Implicit Bias In The Jeffmd Case-Based Learning Curriculum, Abigail Wetzel, Denine Crittendon, Mph, Rosemary Frasso, Phd, Andres Fernandez, Md, Susan Truong, Md, Traci Trice, Md

Phase 1

Purpose: Race is related to health disparities, not to genetic predispositions, but race is often presented to medical students as a biological variable. At Sidney Kimmel Medical College, the curriculum has never been reviewed to analyze how race is presented to students. This Scholarly Inquiry project aims to analyze how the case-based learning (CBL) narratives presented to medical students perpetuate or challenge implicit biases in medicine, especially in regard to race. This paper will describe the methods of the project, which can serve as a model for other institutions to review their own curricula.

Methods: Thirty-nine CBL cases from the …

Jefferson Digital Commons Quarterly Report: October-December 2019, Kelsey Duinkerken, Msi, James Copeland, Libt, Larissa Gordon, Mslis, Med, Ma

Jefferson Digital Commons Reports

This quarterly report includes:

• Articles
• Dean's Research Development Lunch Conference
• Dissertations
• Educational Materials
• From the Archives
• Grand Rounds and Lectures
• Journals and Newsletters
• Population Health Presentation Materials
• Posters
• Reports
• Symposiums
• What People are Saying About the Jefferson Digital Commons

Public Health Bachelor's Degree Academic Program Review, 2019, College Of Science And Mathematics, Wright State University

Academic Program Review Reports - All

This report reviews Wright State University's Public Health Bachelor's Degree program, specifically looking at the time from Fall 2015 to Fall 2019.

The Effect Of Advanced Glycation End Products (Ages) On Macrophages, And Their Implications For Prostate Cancer Progression, Callan C. Frye

MUSC Theses and Dissertations

Advanced glycation end products (AGEs) are sugar-protein adducts that may worsen cancer severity and may contribute to health disparity outcomes among prostate cancer (PCa) patients. AGEs are formed endogenously via a slow, nonenzymic reaction; however, AGEs are also formed in foods as a result of subjecting meats and sugars to high-heat conditions. AGEs are therefore prolific in the Western Pattern Diet (WPD), which has become increasingly common among industrialized nations. Previous work has identified dietary AGE as contributing to MYC-driven PCa progression, and the dependence of this process upon stromal receptor for advanced glycation end products (RAGE). This study therefore …

Productive Separations: Emergent Governance Of Reentry Labor, Shreya Subramani

Fordham Urban Law Journal

No abstract provided.

Karen Rothenberg: Prescient, Perceptive, Persistent And Passionate, Diane E. Hoffmann

Journal of Health Care Law and Policy

No abstract provided.

2020 Comencement Program, Morehead State University. Registrar's Office.

Morehead State Commencement Programs

2020 commencement programs of Morehead State University.

Administration Of Graduate Studies At Public Master’S Institutions In The United States, Brent Graves

Journal Articles

No abstract provided.

Preventing Sexual Violence: Alternatives To Worrying About Recidivism, Eric S. Janus

Faculty Scholarship

How can it be that in the era in which almost one million Americans are on sex offender registries—most of whom are publicly stigmatized on websites, banished from their homes, shunned from their jobs, prevented from uniting with their families and traveling internationally, forced into homelessness, all of which increases their risk for suicide, and shames their spouses and children, even if their offenses occurred long in the past—that the #MeToo movement would explode, revealing widespread sexual misconduct against women, by powerful men, protected by iconic institutions? How can we have had three decades of the most aggressive, “spare-no-expense” laws …

“Innocent Bystanders”: White Guilt And The Destruction Of Native Americans In Us Literature, 1824-1830, Noor Al-Attar

Theses and Dissertations--English

Stereotypes describing the Native Peoples as lacking in many attributes such as religion, civilization, self-control, and even family bonds originated in the early years of contact, popularized through captivity narratives, and used in nineteenth century writings to justify the “vanishing” of the Native people. My dissertation adds to the discussion of myth of the Vanishing American by focusing on overlooked representations of Native illness. Illness, a shared human experience, was preserved for white characters in white authors’ writings. Ailing Native peoples were either denied any stories narrating this experience of human vulnerability or were depicted as resorting to superstitious and …

Medical Utilization In The Context Of Culture: Analyzing The Concepts, Benefits And Drawbacks Of Sri Lankan Biomedical And Ayurvedic Healthcare, Miriah Rajaguru

All Graduate Theses, Dissertations, and Other Capstone Projects

Sri Lanka is an island inhabited by a culture more than 5,000 years old. The primary medicine utilized by this culture in precolonial times is known as Ayurveda. During colonization, Sri Lanka was introduced to biomedicine and forced to negate Ayurvedic medicine. Throughout the years, rather than abandoning their indigenous medical practice, Sri Lanka incorporated biomedicine and Ayurvedic medicine into their medically plural society. Today, Sri Lankans utilized both medicines for different ailments, concerns and conditions. Utilizing a variety of anthropological methods and theories, this study gathered qualitative information from 39 Sri Lankan informants. These informants were recruited to find …

Comparison Of Minnesota Teachers’ Sex Education Instructional Practices With Professional Preparation And Development, Lisa Brichacek

All Graduate Theses, Dissertations, and Other Capstone Projects

Nationally, many health teachers have not received health education training. Minnesota does not require sexual health training or sexual health professional development for health education teachers. School Health Profiles 2018 data was analyzed to determine who teaches sexual health education in Minnesota, what they teach, how they teach it, and their desired future professional development. Teachers who received pre-service health education training were compared with teachers who received other pre-service preparation. Additionally, teachers who received sexual health professional development were compared with teachers who did not receive sexual health professional development. Data analyses included crosstabs with Phi and Cramer’s V …

Fieldwork Educator Preparedness: A Study Examining The Effect Of An Online Learning Module On The Perceived Preparedness Levels Of Fieldwork Educators, Breanna Chycinski

Occupational Therapy Doctorate Capstone Projects

The purpose of this research project was to compare how the implementation of a learning module, titled The Fieldwork Educator Competency Module altered the perceived level of preparedness of occupational therapy (OT) practitioners related to their role as a fieldwork educator (FWEd) for Level II OT students. A quantitative, pre-experimental, one group pretest-posttest research design was used. All components of the research took place in an online, virtual setting. Participants were OT practitioners who were eligible to be a Level II FWEd. Results indicate a positive change for at least one participant for each question of the survey. The largest …

Salamander Stress And Duress: The Relationship Between Cort, Autotomy And Regeneration, And Exploratory Behaviour, Aaron M. Sullivan, Jacquelyn L. Lewis

Faculty Publications - Department of Biological & Molecular Science

Responses to stress are generally mediated through the production of glucocorticoids by the hypothalamic-pituitary-adrenal (or -interrenal) axis. The prolonged production of stress hormones can contribute to delayed wound healing and growth, but little is known about their influence on regeneration following tail autotomy, or exploratory behaviour in autotomized individuals. Here we examined the relationship between stress, re-generation, and exploratory behaviour in Allegheny Mountain dusky salamanders (Desmognathus ochrophaeus) by manipulating corticosterone (CORT) levels via cutaneous patch. First, we measured tail regeneration in salamanders with elevated CORT for 13 weeks after the induction of tail autotomy. Test subjects received a weekly patch …

Evaluating Volatile Organic Compound Emissions From Cross-Laminated Timber Bonded With A Soy-Based Adhesive, Michael Yauk, Jason Stenson, Micah T. Donor, Kevin Van Den Wymelenberg

Faculty Publications - Department of Biological & Molecular Science

Volatile organic compound (VOC) emissions from indoor sources are large determinants of the indoor air quality (IAQ) and occupant health. Cross-laminated timber (CLT) is a panelized engineered wood product often left exposed as an interior surface finish. As a certified structural building product, CLT is currently exempt from meeting VOC emission limits for composite wood products and confirming emissions through California Department of Public Health (CDPH) Standard Method testing. In this study, small chamber testing was conducted to evaluate VOC emissions from three laboratory-produced CLT samples: One bonded with a new soy-based cold-set adhesive; a second bonded with a commercially …

Rapid Determination Of Activation Energies For Gas-Phase Protein Unfolding And Dissociation In A Q-Im-Tof Mass Spectrometer, Micah T. Donor, Samantha O. Shepherd, James S. Prell

Faculty Publications - Department of Biological & Molecular Science

Ion mobility-mass spectrometry has emerged as a powerful tool for interrogating a wide variety of chemical systems. Collision-induced unfolding (CIU), typically performed in time-of-flight instruments, has been utilized to obtain valuable qualitative insight into protein structure and illuminate subtle differences between related species. CIU experiments can be performed relatively quickly, but unfolding energy information obtained from them has not yet been interpreted quantitatively. While several methods can determine quantitative dissociation energetics for small molecules, clusters, and peptides, these methods have rarely been applied to proteins, and never to study unfolding. Here, we present a method to rapidly determine activation energies …

Protonation Isomers Of Highly Charged Protein Ions Can Be Separated In Faims-Ms, J. Diana Zhang, Micah T. Donor, Amber D. Rolland, Michael G. Leeming, Huixin Wang, Adam J. Trevitt, K.M. Mohibul Kabir, James S. Prell, William A. Donald

Faculty Publications - Department of Biological & Molecular Science

High-field asymmetric waveform ion mobility spectrometry-mass spectrometry (FAIMS-MS) can resolve over an order of magnitude more conformers for a given protein ion than alternative methods. Such an expansion in separation space results, in part, from protein ions with masses of >29 kDa undergoing dipole alignment in the high electric field of FAIMS, and the resolution of ions that adopt pendular vs free rotor states. In this study, FAIMS-MS, collision-induced dissociation (CID), and travelling wave (TW) IMS-MS were used to investigate the pendular and free rotor states of protonated carbonic anhydrase II (CAII, 29 kDa). The electrospray ionization additive 1,2-butylene carbonate …

Increasing Collisional Activation Of Protein Complexes Using Smaller Aperture Source Sampling Cones On A Synapt Q-Im-Tof Instrument With A Stepwave Source, Jesse W. Wilson, Micah T. Donor, Samantha O. Shepherd, James S. Prell

Faculty Publications - Department of Biological & Molecular Science

Quadrupole-ion mobility-time-of-flight (Q-IM-TOF) mass spectrometers have revolutionized investigation of native biomolecular complexes. High pressures in the sources of these instruments aid transmission of protein complexes through damping of kinetic energy by collisional cooling. Since adducts are removed through collisional heating (declustering), excessive collisional cooling can prevent removal of non-specific adducts from protein ions, leading to inaccurate mass measurements, broad mass spectral peaks, and obfuscation of ligand binding. We show that reducing the source pressure using smaller aperture source sampling cones (SC) in a Waters Synapt G2-Si instrument increases protein ion heating by decreasing collisional cooling, providing a simple way to …

Correlated Clusters Of Closed Reaction Centers During Induction Of Intact Cells Of Photosynthetic Bacteria, Péter Maróti, István A. Kovács, Mariann Kis, James L. Smart, Ferenc Iglói

Faculty Publications - Department of Biological & Molecular Science

Antenna systems serve to absorb light and to transmit excitation energy to the reaction center (RC) in photosynthetic organisms. As the emitted (bacterio)chlorophyll fluorescence competes with the photochemical utilization of the excitation, the measured fluorescence yield is informed by the migration of the excitation in the antenna. In this work, the fluorescence yield concomitant with the oxidized dimer (P+ ) of the RC were measured during light excitation (induction) and relaxation (in the dark) for whole cells of photosynthetic bacterium Rhodobacter sphaeroides lacking cytochrome c2 as natural electron donor to ­P+ (mutant cycA). The relationship between the fluorescence yield and …

Salt Marsh Management: Monitoring Threats And Implementing Climate Adaptation Initiatives, Danielle C. Perry

Open Access Dissertations

Salt marshes serve as important coastal resources both economically and environmentally and are particularly susceptible to the negative implications of climate change. As climate change impacts become more prevalent, it is important to understand how salt marshes will respond. Two climate change impacts on salt marshes are macroalgae inundation and accelerated relative sea-level rise (hereafter referred to as SLR). Due to warming temperatures and sea level rise, salt marshes can be inundated by macroalgae through tidal movement, and the impacts of this excess macroalgal accumulation are poorly understood. Sea level rise also has negative implications on salt marsh by leading …

Determining Impacts Of Iron Limitation On Diatom Chloroplast Metabolism And Physiology, Kristofer Gomes

Open Access Dissertations

Within the marine ecosystem, phytoplankton such as diatoms experience regions of nutrient limitation including macronutrients such as nitrogen, silica, and phosphate. In regions with high concentration of these nutrients but low chlorophyll a concentrations (HNLCs), phytoplankton are limited for micronutrients such as iron (Fe) an element that serves as a co-factor in proteins involved in cellular redox reactions. Of particular interest for diatoms is the Fe requirement of cellular processes localized to the chloroplast. These Fe requirements are in multiple metabolic pathways such as light reactions of photosynthesis that generate the necessary energy and reducing power necessary for anabolic processes …

Modulation Of Receptor Interacting Protein Kinase 1 (Ripk1) Signaling During Dengue Virus Infection, Darshika Jeewanthi Udawatte

Open Access Dissertations

Dengue virus (DENV) infection is the most prevalent arthropod-borne virus disease. There are estimated 100 million symptomatic cases of dengue disease per year worldwide. A lack of effective antiviral therapies or vaccines makes DENV infections a major global health concern. Therefore, understanding the viral and host factors contributing to the disease pathogenesis is critical. DENV has been shown to effectively counteract host innate immune mechanisms. In particular, DENV protease, NS3 has emerged as an inhibitor of host cellular signaling by targeting crucial host proteins. Here we identified RIPK1 as a novel target of DENV NS3. RIPK1 is a central mediator …

The Frequent Flyer’S Guide To Migration: How Songbird Metabolism And Oxidative Status Respond To Endurance Flight And Diet Quality, Kristen J. Demoranville

Open Access Dissertations

Migratory songbirds are among the best high-performance endurance athletes on the planet and during their annual peregrinations they are particularly vulnerable to oxidative damage because they maintain relatively high metabolic rates at rest, they are able to fly for long durations while operating at 9 times their resting metabolic rate, and they rely on fats to fuel this exercise which increases reactive oxygen species (ROS) formation. The concept of evolutionary economic design of physiological systems considers such phenotypic flexibility in metabolic and antioxidant responses adaptive in that it allows birds to match the capacity of key physiological systems to prevailing …

Design And Validation Of Smart Fabric Sensors Based On Semiconductive Polymer Composites, Arar Alkhader

Open Access Dissertations

Smart Fabric Sensors (SFSs) have recently gained much interest as they are convenient as a long-term wearable as well as for their simplicity, lightweight, low manufacturing cost, high sensitivity, and flexibility. Semiconductive polymer composites SCPCs can be used as smart fabrics to create force or pressure sensors based on the piezoresistive effect, however, their wider application is limited due to their narrow sensing range, hysteresis and output drift issues as well as due to the lack of literature studying their voltage response related errors especially under high impact forces. In this Doctoral research, eight proposed designs of a smart fabric …

Function And Variability Of Metabolic Networks, Keith Dufault-Thompson

Open Access Dissertations

The study of bacterial growth has highlighted the importance of metabolism in how microorganisms have evolved and how they survive in different conditions. The introduction of next-generation sequencing methods has allowed for the study of metabolism in new ways by predicting metabolic phenotypes based on gene annotations. The development of GEnome-scale models (GEMs) of metabolism from these annotations has provided another method to investigate microbial metabolism. An overview of GEM development and simulation methods is provided in Appendix I.

In Manuscript I, a GEM was developed for the psychrotolerant, piezotolerant, deep-sea bacteria Shewanella piezotolerans WP3. Despite the broad differences in …

The Biogeography Of Antarctic Marine Diatoms And Their Response To Varied Iron Concentrations, Laura Z. Holland

Open Access Dissertations

Diatoms are a group of highly diverse photosynthetic eukaryotes that account for about 40% of oceanic primary production. In large areas of the ocean deemed high nitrate, low chlorophyll (HNLC) regions, dissolved macronutrient (nitrogen, phosphorus, silica) concentrations are high in the surface but diatom biomass is consistently low. The Southern Ocean (SO) is the largest of these environments, and when exogenous Fe is supplied to this region, large diatoms blooms and subsequent carbon export are promoted. As such, SO diatoms are major players in the global carbon cycle and were the focus of this dissertation. This work sought to determine …

Northern Plains Resource Council V. United States Army Corps Of Engineers, Liz M. Forster

Public Land & Resources Law Review

Environmental activist and indigenous rights groups have challenged the validity of the Keystone XL Pipeline since its initial approval in 2010. In April 2020, less than a month after crews broke ground, the opposing groups notched a major win when the United States District Court for the District of Montana revoked a key permit for the project on the grounds that the United States Army Corps of Engineers had inadequately assessed the pipeline’s impact on endangered species.

Constitutionalizing Nature's Law: Dignity And The Regulation Of Biotechnology In Switzerland, James Toomey

Elisabeth Haub School of Law Faculty Publications

The Swiss Constitution was amended by referendum in 1992 to include two unique provisions: Article 119, which imposes strict limits on genetic and reproductive technologies in humans in order to protect ‘human dignity’, and Article 120, which commits the Swiss federal government to limiting genetic technologies in non-human species on the basis of the ‘dignity of the creature’. This article analyzes the role of ‘dignity’ as a limit on biotechnologies in the Swiss constitutional order. It concludes that the understanding of dignity the constitution embraces codifies a contestable metaphysical theory of value at the constitutional level. Specifically, the Swiss constitutional …

Dehumanization 'Because Of Sex': The Multiaxial Approach To The Title Vii Rights Of Sexual Minorities, Shirley Lin

Elisabeth Haub School of Law Faculty Publications

Although Title VII prohibits discrimination against any employee “because of such individual’s . . . sex,” legal commentators have not yet accurately appraised Title VII’s trait and causation requirements embodied in that phrase. Since 2015, most courts assessing the sex discrimination claims of LGBT employees began to intentionally analyze “sex” as a trait using social-construction evidence, and evaluated separately whether the discriminatory motive caused the workplace harm. Responding to what this Article terms a “doctrinal correction” to causation within this groundswell of decisions, the Supreme Court recently issued an “expansive” and “sweeping” reformulation of but-for causation in Bostock v. Clayton …